CN105009944A - Fast inoculation method for mushroom stick - Google Patents
Fast inoculation method for mushroom stick Download PDFInfo
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- CN105009944A CN105009944A CN201510481057.2A CN201510481057A CN105009944A CN 105009944 A CN105009944 A CN 105009944A CN 201510481057 A CN201510481057 A CN 201510481057A CN 105009944 A CN105009944 A CN 105009944A
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- inoculation
- bacterium rod
- account
- buttress
- plastic foil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a fast inoculation method for a mushroom stick. The fast inoculation method includes the following steps that firstly, greenhouse treating and hearth outlet stacking are conducted, wherein the ground of a greenhouse is cleaned and tidied, a layer of river sand is spread on the ground, bactericide is sprayed, and a peripheral greenhouse film is sealed; secondly, an inoculation tent is erected, wherein the mushroom stick is naturally cooled, meanwhile, the inoculation tent is erected to cover a mushroom stick stack, and smoke disinfection is conducted on the interior of the inoculation tent; thirdly, mushroom stick inoculation is conducted, wherein holes are punched in the mushroom stick, aged mycoderma on a strain is removed, the strain is broken off into small pieces to be plugged into the holes, and the mushroom stick stack obtained after strain inoculation and the surface are covered by a mulching film for static cultivation. The fast inoculation method is high in inoculation speed, simple, practical, low in cost and capable of achieving large-scale intensive production.
Description
Technical field
The present invention relates to a kind of inoculation method, particularly the quick inoculation method of a kind of xianggu stick.
Background technology
In Edible Fungi, inoculation is a procedure of most critical, is directly connected to the success or failure of production.Inoculation be also edible Mycota people generally acknowledge one production link that most work consuming is time-consuming, worry.The several methods such as current xianggu stick in all parts of the country inoculation mainly contains inoculating hood, superclean bench, transfer room, the indoor account formula of canopy are inoculated fast.According to edible fungi of china association statistics, the mushroom gross yield of China in 2012 reaches 6,350,000 tons.Each bacterium rod produces about 1.2 jin, mushroom, approximately needs inoculation 10,583,330,000 rod.The mushroom production of China is annual all in increase.At present, the Lentnus edodes of China is to intensive, commercial running future development, the mainly enterprise's transfer room operating desk inoculation of Edible Fungi on a large scale, if publication number is the Chinese patent of CN104145717A, this vaccination ways, investment is large, and conditional request is high, single inoculation efficiency is lower, is unfavorable for extensively promoting and intensive manufacture.In addition, the intensive Lentnus edodes in small towns, it is nonstandard that the indoor account formula of the canopy of employing inoculates operation fast, and pollution rate is high, causes cost to increase.
Summary of the invention
In order to solve the aforementioned problems in the prior, the invention provides the quick inoculation method of a kind of xianggu stick, comprising the following steps: the quick inoculation method of a kind of xianggu stick, comprises the following steps:
(1) canopy room processes and goes out stove piling
First carry out cleaning to canopy room floor and arrange, and spreading one deck river sand on the ground, spraying fungicide on river sand, canopy film is sealed; Then spreading on the river sand needing code bacterium rod buttress, it is indoor that canopy sent into by the bacterium rod that will complete disinfecting action, and pile up form multiple bacterium rod buttress on mulch film;
(2) inoculation account is built
Bacterium rod cools naturally, builds inoculation account to cover bacterium rod buttress and to carry out smog sterilization to inoculation account inside simultaneously;
(3) bacterium rod inoculation
When bacterium rod temperature is down to below 35 DEG C to the sterilization of bacterium rod, then to punch on each bacterium rod cave, remove the old mycoderma on bacterial classification, breaking into fritter again fills in hole, inoculate the bacterium rod piling capping plastic foil static gas wave refrigerator of bacterial classification, control temperature in bacterium rod buttress, when bacterial classification grows to preliminary dimension, turn over buttress and Jing Zimaduo.
As preferably, the sterilization in above-mentioned steps (2) in inoculation account, lights sterilizing fumicants produce smog, and airtight 1.5 hours ~ 3 hours, then give off poisonous gas.
As preferably, described inoculation account adopts thickness to be that the plastic foil of 10 is made, and uses support bar jack-up when depressed area appears in described inoculation account top.
As preferably, in above-mentioned steps (1), described bacterium rod selects polyethylene from high pressure process bag cylinder to be cultivation bag, dresses up material and forms described bacterium rod.
As preferably, the length of described bacterium rod is 41cm ~ 45cm, and girth is 30cm, and the hole on each described bacterium rod is 2 ~ 4, the dark 3cm ~ 5cm in described hole, diameter 2cm ~ 3cm, and the hole depth is consistent and be uniformly distributed.
As preferably, in above-mentioned steps (1), 1:200 is formulated in mass ratio by 50% carbendazol wettable powder and water for described bactericide, and the sprinkling consumption of bactericide is 100g/m
2~ 300g/m
2.
As preferably, in above-mentioned steps (1), the thickness of the described river sand that ground is spread is 1cm ~ 4cm.
As preferably, described sterilizing fumicants is sodium dichloro cyanurate.
As preferably, the building method of described inoculation account is: according to quantity and the size cutting plastic foil of bacterium rod buttress, and the length of plastic foil is greater than length and the height sum of inoculation account, and the width of plastic foil is greater than width and the height sum of inoculation account; Then four stress points are chosen, tether on four stress points is also bonded to the top of canopy room, and then correspondence is chosen stress point and is bonded to the top of canopy room by rope between adjacent two summits, the plastic foil held up by stress point forms the end face of inoculation account, the plastic foil that hangs down forms the side of inoculation account, and the height that the height of the plastic foil of side is greater than inoculation account is with sealing inoculation account under enabling to fall to ground plastic foil and being embedded in mulch film.
As preferably, when piling, pile up 4-6 in each inoculation account and pile up neatly bacterium rod, every height of embankment 1.3m ~ 1.5m, often piles up neatly long 8m-10m, and the width of bacterium rod buttress is the length of bacterium rod; Inoculation account height is 1.9m ~ 2.1m; Bacterium rod buttress distance inoculation account 15cm ~ 25cm.
Compared with prior art; beneficial effect of the present invention is: what the quick inoculation method of xianggu stick of the present invention adopted is canopy indoor account formula semi open model inoculation technique; can in rural area during intensive large-scale production; utilize simple facilities and equipment; division of labor formula operates; reach effect of inoculation good fast; simple and practical; pollution rate is low; the high needs meeting extensive intensive manufacture of bacterial classification survival rate, and compared with the inoculation of batch production, invest little; inoculation speed is fast, has saved production cost.
Accompanying drawing explanation
Fig. 1 is the piling schematic diagram in the quick inoculation method of a kind of xianggu stick that the present invention relates to;
Fig. 2 is the preparation method schematic diagram of the inoculation account in the quick inoculation method of a kind of xianggu stick that the present invention relates to;
Fig. 3 is the structural representation of the bacterium rod in the quick inoculation method of a kind of xianggu stick that the present invention relates to.
Embodiment
Describe in further detail embodiments of the invention below in conjunction with the drawings and specific embodiments, following content relates to the specific embodiment of the quick inoculation method of a kind of xianggu stick.The quick inoculation method of this xianggu stick comprises the following steps:
(1) canopy room processes and goes out stove piling
First carry out cleaning to canopy room 1 (see Fig. 1) ground and arrange, require canopy room 1 fully dryness in the sun, ground is clean, the river sand of one deck 1cm ~ 4 thickness is spread on ground, river sand has the comparatively large and uniform feature of sand particle size, and impure less, and has good moisture absorption and desiccation, the miscellaneous bacteria on ground can be isolated, preventing pollution bacterium rod 2 (structure of bacterium rod 2 refers to Fig. 3).Certainly, river sand also can replace by other husky classes, as long as ensure moisture absorption, drying and the effect every bacterium.The thickness of river sand depending on the surface state of canopy room 1, such as, can select 1cm, 2cm, 3cm, 3.3cm etc.
Then, spraying fungicide on river sand again, to kill except the miscellaneous bacteria in river sand, bactericide can be selected multiple, as Methylpartricin Sodium Laurylsulfate or the active ingredient carbendazol wettable powder that is 50% adds water formulated etc., as select active ingredient be 50% carbendazol wettable powder as bactericide time, need carbendazol wettable powder dilute with water 200 times, namely solute is the carbendazol wettable powder of 50%, and solvent is water, and the mass ratio of solute and solvent is 1:200.The sprinkling consumption of bactericide, can at 100g/m depending on the clean-up performance of river sand
2~ 300g/m
2select in scope, as 100g/m
2, 200g/m
2, 300g/m
2etc.; The canopy film of surrounding is sealed.
Before piling, first the thick mulch film in river sand upper berth on the ground of bacterium rod buttress 3 is piled up in plan, mulch film is formed bacterium rod 2 piling regions, the bacterium rod 2 completing disinfecting action is sent into by designing requirement while hot in the piling region of canopy room 1 that (the present embodiment is described to inoculate 5000 bacterium rods 2, the specification of bacterium rod 2 will hereafter be introduced), and on mulch film, pile up into multiple bacterium rod buttress 3 on request, particularly, bacterium rod 2 can divide 4-6 arrangement to be set to 4-6 bacterium rod buttress 3, each bacterium rod buttress 3 high 1.3m ~ 1.5m, height is to facilitate operative to be advisable, be equivalent to each bacterium rod buttress 3 and put about 15 layers of bacterium rod 2, each bacterium rod buttress 3 is about 8m-10m.Become 4 discharge of bacteria rod buttress 3 for 5000 bacterium rods 2 yards, in canopy room 1, piling floor map is see Fig. 1, and the width in FIG according to canopy room 1 piles up 4 discharge of bacteria rod buttress 3, often discharges the bacterium rod 2 of equal number, the distance L between two rows of both sides
1about 0.15m can be set to, the distance L on outermost two rows and corresponding booth border
2at least 0.5m can be set to, the distance L between middle two rows
3about 2m (this distance to facilitate follow-up turn over buttress) can be set to.Wherein, when selecting bacterium rod 2, the polyethylene from high pressure process bag cylinder selecting girth 30cm × long 60cm × thick 50 μm is cultivation bag, and dress up material and form bacterium rod 2, the column length 43cm ± 2cm of bacterium rod 2, weight in wet base is about 2.2kg ± 0.1kg.
(2) inoculation account is built
Naturally cool after bacterium rod 2 pilings, and detect the temperature of bacterium rod 2 in real time, (temperature that bacterium rod 2 declines is depending on external environment can to start to prepare inoculation when the temperature of bacterium rod 2 is down to below 35 DEG C, if but the too high inoculation of the temperature of bacterium rod 2, bacterial classification not easily survives, if but bacterium rod 2 decline temperature controlled too low, the time then needed is longer, cause miscellaneous bacteria to be grown, contaminated bacteria rod 2, affect the cultivation of follow-up bacterial classification; While bacterium rod 2 Temperature fall, make inoculation account, wherein inoculate account and adopt thickness to be that the plastic foil 4 of 10 is made, according to quantity and the size cutting plastic foil of bacterium rod buttress 3, the length L of the plastic foil after cutting
4the length L of inoculation account should be greater than
5with height sum, the width W of plastic foil
1the width W of inoculation account should be greater than
2with height sum.Such as, when piling up four discharge of bacteria rod buttress 3 as shown in Figure 1 in canopy room 1, (length of bacterium rod buttress 3 is 8 meters, and the width of bacterium rod buttress 3 is 0.5 meter, the distance L of middle two bacterium rod buttress 3
3distance be 2m, the distance L of two bacterium in both sides rod buttress 3
1for 0.15m) time, m plastic foil adopts the plastic foil of normal width, and its width is 10m, and the length of cutting increases 5m again, i.e. L on bacterium rod buttress 3 length
4for 13m, after having measured size, carry out cutting; Fig. 2 shows the plastic foil for making after the cutting of inoculation account, as shown in Figure 2, choose six impetus A, B, C, D, E and F on the plastic film, and be bonded to the top of canopy room 1 at six impetus place tether respectively, and ensure that in inoculation account, space keeps being highly 1.9m ~ 2.1m, as 2m (this height is that conveniently operator can to stand erectly physical task in inoculation account), in the present embodiment, inoculate the length L of account
5for bacterium rod buttress length 8m adds 15cm ~ 25cm, the width W of inoculation account
2for 5m, namely bacterium rod buttress 3 and should inoculate the distance retaining 15cm ~ 25cm between account.Choosing of stress point quantity determines according to the size of inoculation account, and in order to ensure that inoculation account can be risen by support, answering minimum is six.The plastic foil that held up by stress point forms the end face of inoculation account, and the plastic foil hung down forms the side of inoculation account, and the height that the height of the plastic foil of side is greater than inoculation account is with sealing inoculation account under enabling to fall to ground plastic foil and being embedded in mulch film.Side falls to ground plastic foil and also can push down with bacterium rod 2 and obturage, and the surrounding of inoculation account and booth border are at least at a distance of 0.5m, and inoculation account covers in inoculation the previous day; In addition, the many long support bar (not shown)s for about 2.2m are prepared in inoculation account, support bar top towel cotton wraps (preventing from poking plastic foil), when inoculating when depressed area appears in the top of account for supporting the depressed area at inoculation account top during inoculation.
After inoculation account is built, select bacterial classification, calculate the consumption (every kilogram of bacterial classification connects 50 bacterium rods 2) of bacterial classification, keep accounts after washing with the potassium permanganate liquids of 3000 times interior for subsequent use, prepare card punch, blade, alcohol-pickled towelette, refuse bag, 75% alcohol, plastic basin, a volume broadly film (10m is loose) etc., in inoculation first 4 hours, carry out disinfection in inoculation account, the present embodiment adopts lights the mode that sterilizing fumicants produces smog sterilization, sterilizing fumicants can adopt sodium dichloro cyanurate, for the size inoculating account in the embodiment of the present invention, need to use sodium dichloro cyanurate 25 box that specification is 40g/ box, then light the sterilization of this bacterium fumicants generation smog (can be placed in canister and light, as ironware), account will be inoculated airtight 1.5 hours ~ 3 hours, as 2.5 hours, then bed-curtain side (side away from doorway, canopy room) is opened, give off poisonous gas, laggard inoculation account inoculation in about 1 hour-1.5 hours.
(3) bacterium rod inoculation
Operating personnel wear clean clothing and enter canopy room 1, here preferably special clothing is equipped with, and operating personnel should wear masks, emgloves, shoe cover, beginning to be seeded, 1 people first soaks 75% wipes of alcohol bacterium rod 2 sterilization with towel, as shown in Figure 3, each bacterium rod 2 punches cave 5 is 2 ~ 4, the dark 3cm ~ 5cm in hole 5, and diameter is 2cm ~ 3cm, require that the consistent hole 5 of the depth is evenly distributed, the length of the bacterium rod 2 shown in the present embodiment is 45cm, and girth is 30cm, and hole 5 is 3, the dark 5cm in hole 5, diameter is 2.5cm.3 holes 5 are arranged in a row, and a hole 5 is wherein positioned at the middle part of bacterium rod 2, and the spacing in adjacent two holes 5 is 17.5cm, and two holes 5 being positioned at both sides are 5cm apart from the distance at the two ends of bacterium rod 2.
1 people processes bacterial classification and removes old mycoderma outside bacterial classification, to process in time as found that there is pollution, bacterial classification is broken into fritter and is filled in hole 5 by 3 people, bacterial classification is moderate by the size of breaking, make not stay gap in hole 5,2 people remove bacterium rod 2 pilings, arrive taking over bacterium rod 2 buttress of planting after one's death, more than operation forms pipelining, until bacterium rod 2 has all been inoculated; Finally, bacterium rod buttress 3 surface mulch film capping static gas wave refrigerator, detects the temperature in bacterium rod buttress 3 with simple glass column temperature meter, if temperature anomaly raises, checks pollution condition in time.Wherein, when to grow to diameter be 5cm-10cm to bacterial classification, buttress can be turned over and pick up assorted, Jing Zimaduo.
Representative embodiment of the present invention obtains detailed description.These detailed descriptions only give those skilled in the art's further detailed content, for the preferred aspect carried out an invention, and can not limit scope of the present invention.Only have the right requirement for determining protection scope of the present invention.Therefore, the characteristic sum step in aforementioned detailed description in conjunction with dispensable for implementing the present invention in the broadest scope, and alternatively only instruction is provided to the representative embodiment of special detailed description of the present invention.In addition, in order to obtain of the present invention being attached with by embodiment, the various different feature providing instruction in the description combines by various ways, but these modes are not exemplified out especially.
Claims (10)
1. the quick inoculation method of xianggu stick, comprises the following steps:
(1) canopy room processes and goes out stove piling
First carry out cleaning to canopy room floor and arrange, and spreading one deck river sand on the ground, spraying fungicide on river sand, canopy film is sealed; Then spreading on the river sand needing code bacterium rod buttress, it is indoor that canopy sent into by the bacterium rod that will complete disinfecting action, and pile up form multiple bacterium rod buttress on mulch film;
(2) inoculation account is built
Bacterium rod cools naturally, builds inoculation account to cover bacterium rod buttress and to carry out smog sterilization to inoculation account inside simultaneously;
(3) bacterium rod inoculation
When bacterium rod temperature is down to below 35 DEG C to the sterilization of bacterium rod, then to punch on each bacterium rod cave, remove the old mycoderma on bacterial classification, breaking into fritter again fills in hole, inoculate the bacterium rod piling capping plastic foil static gas wave refrigerator of bacterial classification, control temperature in bacterium rod buttress, when bacterial classification grows to preliminary dimension, turn over buttress and Jing Zimaduo.
2. the quick inoculation method of xianggu stick according to claim 1, is characterized in that: the sterilization in above-mentioned steps (2) in inoculation account, lights sterilizing fumicants produce smog, and airtight 1.5 hours ~ 3 hours, then give off poisonous gas.
3. the quick inoculation method of xianggu stick according to claim 2, is characterized in that: described inoculation account employing thickness is that the plastic foil of 10 is made, and uses support bar jack-up when depressed area appears in described inoculation account top.
4. the quick inoculation method of the xianggu stick according to any one of claim 1-3, is characterized in that: in above-mentioned steps (1), and described bacterium rod selects polyethylene from high pressure process bag cylinder to be cultivation bag, dresses up material and forms described bacterium rod.
5. according to the quick inoculation method of xianggu stick that claim 4 is stated, it is characterized in that: the length of described bacterium rod is 41cm ~ 45cm, and girth is 30cm, hole on each described bacterium rod is 2 ~ 4, dark 3cm ~ the 5cm in described hole, diameter 2cm ~ 3cm, the hole depth is consistent and be uniformly distributed.
6. the quick inoculation method of xianggu stick according to claim 1, it is characterized in that: in above-mentioned steps (1), 1:200 is formulated in mass ratio by 50% carbendazol wettable powder and water for described bactericide, and the sprinkling consumption of bactericide is 100g/m
2~ 300g/m
2.
7. the quick inoculation method of xianggu stick according to claim 6, is characterized in that: in above-mentioned steps (1), and the thickness of the described river sand that ground is spread is 1cm ~ 4cm.
8. the quick inoculation method of xianggu stick according to claim 2, is characterized in that: described sterilizing fumicants is sodium dichloro cyanurate.
9. the quick inoculation method of xianggu stick according to claim 3, it is characterized in that: the building method of described inoculation account is: according to quantity and the size cutting plastic foil of bacterium rod buttress, the length of plastic foil is greater than length and the height sum of inoculation account, and the width of plastic foil is greater than width and the height sum of inoculation account; Then four stress points are chosen, tether on four stress points is also bonded to the top of canopy room, and then correspondence is chosen stress point and is bonded to the top of canopy room by rope between adjacent two summits, the plastic foil held up by stress point forms the end face of inoculation account, the plastic foil that hangs down forms the side of inoculation account, and the height that the height of the plastic foil of side is greater than inoculation account is with sealing inoculation account under enabling to fall to ground plastic foil and being embedded in mulch film.
10. the quick inoculation method of xianggu stick according to claim 1, is characterized in that: when piling, and pile up 4-6 in each inoculation account and pile up neatly bacterium rod, every height of embankment 1.3m ~ 1.5m, often piles up neatly long 8m-10m, and the width of bacterium rod buttress is the length of bacterium rod; Inoculation account height is 1.9m ~ 2.1m; Bacterium rod buttress distance inoculation account 15cm ~ 25cm.
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|---|---|---|---|
| CN201510481057.2A CN105009944A (en) | 2015-08-03 | 2015-08-03 | Fast inoculation method for mushroom stick |
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|---|---|---|---|
| CN201510481057.2A CN105009944A (en) | 2015-08-03 | 2015-08-03 | Fast inoculation method for mushroom stick |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113273432A (en) * | 2021-04-02 | 2021-08-20 | 湖南省绿牧农业科技有限公司 | Inoculation method for edible mushroom culture |
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Application publication date: 20151104 |
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