CN113273432A - Inoculation method for edible mushroom culture - Google Patents

Inoculation method for edible mushroom culture Download PDF

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Publication number
CN113273432A
CN113273432A CN202110359370.4A CN202110359370A CN113273432A CN 113273432 A CN113273432 A CN 113273432A CN 202110359370 A CN202110359370 A CN 202110359370A CN 113273432 A CN113273432 A CN 113273432A
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China
Prior art keywords
inoculation
tent
account
edible mushroom
stack
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CN202110359370.4A
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Chinese (zh)
Inventor
刘昌稳
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Hunan Lvmu Agricultural Technology Co ltd
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Hunan Lvmu Agricultural Technology Co ltd
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Priority to CN202110359370.4A priority Critical patent/CN113273432A/en
Publication of CN113273432A publication Critical patent/CN113273432A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/50Inoculation of spawn

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses an inoculation method for edible mushroom culture, which comprises the following steps: s1, preparing a mosquito net type inoculation net by using an agricultural plastic film, and sealing each edge and each corner by using an electric iron for later use; s2, sealing the inoculation account; s3, transferring the sterilized bacteria stick into an inoculation account; s4, sterilizing the inoculation account; s5, the inoculation personnel enter the inoculation account to do work-division hole digging, inoculation and stack moving; s6, detaching the inoculation tent, and spawn running in the mushroom shed, which relates to the technical field of edible mushroom culture. The invention solves the problem that the inoculation is carried out by an inoculation box or an inoculation chamber commonly used in the production, wherein the inoculation box has too small space, is not beneficial to operation and directly influences the inoculation speed; the inoculation room is too large in space, so that the disinfection is difficult to be thorough, and the inoculated bacteria sticks are required to be transported to a culture room for culture, so that the problem of high strain pollution rate is solved.

Description

Inoculation method for edible mushroom culture
Technical Field
The invention relates to the technical field of edible fungus culture, in particular to an inoculation method for edible fungus culture.
Background
In the production of edible fungi, the inoculation method and the inoculation place are directly related to the success or failure of the strain production.
In the prior art, inoculation is commonly carried out by an inoculation box or an inoculation chamber in production, wherein the inoculation box has too small space, is not beneficial to operation and directly influences the inoculation speed; the inoculation room is too large in space, so that the disinfection is difficult to be thorough, and the inoculated bacteria sticks are required to be transported to a culture room for culture, so that the strain pollution rate is high. Therefore, we invented an inoculation method for edible mushroom culture.
Disclosure of Invention
In order to solve the problem that the inoculation is carried out by an inoculation box or an inoculation chamber commonly used in the production, wherein the space of the inoculation box is too small, the operation is not facilitated, and the inoculation speed is directly influenced; the inoculation room is too large in space, so that the disinfection is difficult to be thorough, the inoculated fungus sticks are required to be transported to a culture room for culture, and the strain pollution rate is high.
In order to achieve the purpose, the invention adopts the following technical scheme: an inoculation method for edible fungus culture comprises the following steps:
s1, preparing a mosquito net type inoculation net by using an agricultural plastic film, and sealing each edge and each corner by using an electric iron for later use;
s2, sealing the inoculation account;
s3, transferring the sterilized bacteria stick into an inoculation account;
s4, sterilizing the inoculation account;
s5, the inoculation personnel enter the inoculation account to do work-division hole digging, inoculation and stack moving;
and S6, detaching the inoculation tent and spawn running in a mushroom shed.
Preferably, in S1, the agricultural plastic has a thin thickness specification of 0.5mm, the inoculation tent occupies an area of 10-12 square meters, and the height is 2.8-3.2 m.
Preferably, in S2, the ground of the inoculation tent is cleaned, a little lime powder is scattered on the ground, 4 top corners of the inoculation tent are respectively hung on the top frame of the mushroom shed by ropes, so that the height of the inoculation tent is 2.5-2.7m, the rest is folded inwards, and the same plastic film is laid on the ground in the inoculation tent to form a relatively closed space.
Preferably, in the S3, 4000-4080 fungus sticks are placed, stacked in 5 rows, and the folded corners of the inoculation tent are compacted by the fungus sticks.
Preferably, in the step S4, 6 aerosol disinfection boxes are placed for sterilization and fumigation for 2h, and one side of the inoculation tent is lifted and deflated for 2 h.
Preferably, in S5, the vaccinator wears sterilized work clothes, hats, masks, gloves and shoe covers, and each work category is operated by a different person.
Preferably, in the step S5, the hole is punched, and 3-4 holes with proper depth are punched on the fungus stick by using a sharp iron stick; inoculating, breaking the strains into blocks with proper sizes, and filling the blocks into the holes; moving the stack, moving the inoculated fungus sticks to the side, placing the inoculation point upwards in a 'well' shape, sleeving thin plastic bags outside 100 inoculated fungus sticks, tying openings, stacking the inoculated fungus sticks in 5 rows to form the stack, finally covering the stack with an agricultural plastic film with the width of 3m, and pressing the periphery and the upper surface of the stack by the fungus sticks sleeved with bags outside.
Compared with the prior art, the invention has the following beneficial effects: according to the invention, the space of the inoculation tent is beneficial to operation, and after inoculation, the inoculation tent is directly stacked, coated and spawn running, so that the step of bagging or hole sealing is omitted, and the inoculation speed is very high; the agricultural plastic film inoculation tent has good sealing performance and thorough disinfection, so that the rate of finished products of the inoculation method can reach 95%; the agricultural plastic film is used for covering and stacking the fungi on the spot, so that the phenomenon that the newly-inoculated fungi block is loosened due to the touch in the processes of bagging or sealing and moving, the field planting is influenced, and the damage and pollution of the fungi bag are reduced.
Drawings
The invention is described in further detail below with reference to the following figures and detailed description:
FIG. 1 is a schematic diagram of the process of the present invention.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Please refer to fig. 1. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions under which the present invention can be implemented, so that the present invention has no technical significance, and any structural modification, ratio relationship change, or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention. In addition, the terms "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for clarity of description, and are not intended to limit the scope of the present invention, and the relative relationship between the terms and the terms is not to be construed as a scope of the present invention.
The invention provides a technical scheme that: an inoculation method for edible fungus culture comprises the following steps:
s1, preparing a mosquito net type inoculation net by using an agricultural plastic film, and sealing each edge and each corner by using an electric iron for later use;
s2, sealing the inoculation account;
s3, transferring the sterilized bacteria stick into an inoculation account;
s4, sterilizing the inoculation account;
s5, the inoculation personnel enter the inoculation account to do work-division hole digging, inoculation and stack moving;
and S6, detaching the inoculation tent and spawn running in a mushroom shed.
In the S1, the agricultural plastic has the specification of thin thickness of 0.5mm, the occupied area of the inoculation tent is 10-12 square meters, and the height is 2.8-3.2 m.
In the S2, the ground of the inoculation tent is cleaned, a little lime powder is scattered on the ground, 4 top corners of the inoculation tent are respectively hung on a top frame of a mushroom shed by ropes, so that the height of the inoculation tent is 2.5-2.7m, the rest top corners are folded inwards, the same plastic film is laid on the ground in the inoculation tent, and a relatively closed space is formed.
In the S3, 4000-.
And in the S4, 6 aerosol disinfection boxes are placed for sterilization and fumigation for 2h, and one side of the inoculation tent is lifted to deflate for 2 h.
In the step S5, the vaccinator wears sterilized work clothes, hats, masks, gloves, and shoe covers, and each work category is operated by a different person.
In the step S5, punching holes, namely, punching 3-4 holes with proper depth on the fungus stick by using an iron stick with one tip; inoculating, breaking the strains into blocks with proper sizes, and filling the blocks into the holes; moving the stack, moving the inoculated fungus sticks to the side, placing the inoculation point upwards in a 'well' shape, sleeving thin plastic bags outside 100 inoculated fungus sticks, tying openings, stacking the inoculated fungus sticks in 5 rows to form the stack, finally covering the stack with an agricultural plastic film with the width of 3m, and pressing the periphery and the upper surface of the stack by the fungus sticks sleeved with bags outside.
When in use, the space of the inoculation tent is beneficial to operation, and after inoculation, the inoculation is directly stacked, coated and spawn running, so that the step of bagging or hole sealing is omitted, and the inoculation speed is high; the number of people is 6, 1 person makes a hole, 3 people inoculate, 2 people move the buttress, can finish 4000 fungus sticks in 3-4h, if the inoculation personnel is many still can raise the efficiency, and the inoculation hood can only connect about 25 fungus sticks per hour.
The agricultural plastic film inoculation tent has good sealing performance and thorough disinfection, so that the rate of finished products of the inoculation method can reach 95%; the agricultural plastic film is used for covering and stacking the fungi on the spot, so that the phenomenon that the newly-inoculated fungi block is loosened due to the touch in the processes of bagging or sealing and moving, the field planting is influenced, and the damage and pollution of the fungi bag are reduced.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (7)

1. An inoculation method for edible fungus culture is characterized by comprising the following steps:
s1, preparing a mosquito net type inoculation net by using an agricultural plastic film, and sealing each edge and each corner by using an electric iron for later use;
s2, sealing the inoculation account;
s3, transferring the sterilized bacteria stick into an inoculation account;
s4, sterilizing the inoculation account;
s5, the inoculation personnel enter the inoculation account to do work-division hole digging, inoculation and stack moving;
and S6, detaching the inoculation tent and spawn running in a mushroom shed.
2. An inoculation method for edible mushroom culture according to claim 1, characterized in that: in the S1, the agricultural plastic has the specification of thin thickness of 0.5mm, the occupied area of the inoculation tent is 10-12 square meters, and the height is 2.8-3.2 m.
3. An inoculation method for edible mushroom culture according to claim 1, characterized in that: in the S2, the ground of the inoculation tent is cleaned, a little lime powder is scattered on the ground, 4 top corners of the inoculation tent are respectively hung on a top frame of a mushroom shed by ropes, so that the height of the inoculation tent is 2.5-2.7m, the rest top corners are folded inwards, the same plastic film is laid on the ground in the inoculation tent, and a relatively closed space is formed.
4. An inoculation method for edible mushroom culture according to claim 1, characterized in that: in the S3, 4000-.
5. An inoculation method for edible mushroom culture according to claim 1, characterized in that: and in the S4, 6 aerosol disinfection boxes are placed for sterilization and fumigation for 2h, and one side of the inoculation tent is lifted to deflate for 2 h.
6. An inoculation method for edible mushroom culture according to claim 1, characterized in that: in the step S5, the vaccinator wears sterilized work clothes, hats, masks, gloves, and shoe covers, and each work category is operated by a different person.
7. An inoculation method for edible mushroom culture according to claim 1, characterized in that: in the step S5, punching holes, namely, punching 3-4 holes with proper depth on the fungus stick by using an iron stick with one tip; inoculating, breaking the strains into blocks with proper sizes, and filling the blocks into the holes; moving the stack, moving the inoculated fungus sticks to the side, placing the inoculation point upwards in a 'well' shape, sleeving thin plastic bags outside 100 inoculated fungus sticks, tying openings, stacking the inoculated fungus sticks in 5 rows to form the stack, finally covering the stack with an agricultural plastic film with the width of 3m, and pressing the periphery and the upper surface of the stack by the fungus sticks sleeved with bags outside.
CN202110359370.4A 2021-04-02 2021-04-02 Inoculation method for edible mushroom culture Pending CN113273432A (en)

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CN202110359370.4A CN113273432A (en) 2021-04-02 2021-04-02 Inoculation method for edible mushroom culture

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Application Number Priority Date Filing Date Title
CN202110359370.4A CN113273432A (en) 2021-04-02 2021-04-02 Inoculation method for edible mushroom culture

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CN113273432A true CN113273432A (en) 2021-08-20

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1494914A (en) * 1973-11-02 1977-12-14 Licencia Talalmanyokat Process for cultivating edible fungi
CN101258816A (en) * 2008-04-25 2008-09-10 赵建荣 Edible mushroom cultivation bar high-efficiency inoculating method
CN105009944A (en) * 2015-08-03 2015-11-04 廊坊市森淼春食用菌有限公司 Fast inoculation method for mushroom stick

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1494914A (en) * 1973-11-02 1977-12-14 Licencia Talalmanyokat Process for cultivating edible fungi
CN101258816A (en) * 2008-04-25 2008-09-10 赵建荣 Edible mushroom cultivation bar high-efficiency inoculating method
CN105009944A (en) * 2015-08-03 2015-11-04 廊坊市森淼春食用菌有限公司 Fast inoculation method for mushroom stick

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
江家余;: "袋栽香菇"蚊帐"式塑料棚接种方法", 新农村, no. 01, pages 13 *
马立芝;吴智艳;侯桂森;: "食用菌规模化生产高效接种技术", 江苏农业科学, no. 05, pages 130 - 131 *

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