CN103688749A - Open type inoculation method for cultivating mushroom with substitute material - Google Patents
Open type inoculation method for cultivating mushroom with substitute material Download PDFInfo
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- CN103688749A CN103688749A CN201310680535.3A CN201310680535A CN103688749A CN 103688749 A CN103688749 A CN 103688749A CN 201310680535 A CN201310680535 A CN 201310680535A CN 103688749 A CN103688749 A CN 103688749A
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Abstract
The invention belongs to the field of edible mushroom technologies, and relates to a cultivation method of a mushroom, in particular to an open type inoculation method in a substitute-material mushroom production process. The open type inoculation method specifically comprises the following steps of putting a compost for cultivating the mushroom into a plastic bag, sterilizing and cooling, sealing a strain bag, a mushroom strain and an inoculation tool together, igniting by an aerial fog disinfector and sterilizing, and inoculating in an open environment by utilizing disinfectant used for inoculation of the mushroom, wherein the disinfectant used for inoculation of the mushroom is formed by 95% ethyl alcohol solution, 50% carbendazol and trichloroisocyanuric acid according to a certain proportion. The open type inoculation method of cultivating the mushroom with the substitute material is capable of completing inoculation of the mushroom without occupying more sterilizing rooms, and has the advantages of simplicity in operation and high inoculation efficiency; the disinfectant used for inoculation of the mushroom is green, environmental-friendly and harmless to a human body.
Description
Technical field
The invention belongs to edible mushroom technical field, relate to the cultivating method of mushroom, specifically a kind of open inoculation method in generation material Lentnus edodes process.
Background technology
Mushroom, has another name called mushroom, fragrant letter, fragrant bacterium, dried mushroom, fragrant wild rice, is the fruit body of Pleurotaceae plant mushroom.Mushroom is second-biggest-in-the-world edible mushroom, and one of Ye Shi China special product, at the title of among the people have " mountain delicacy ".Mushroom is a kind of fungi being grown on timber.Delicious flavour, the fragrance people that oozes, nutritious, have " plant queen " good reputation.Mushroom is also rich in Cobastab group, iron, potassium, provitamin D etc.
Traditional method for cultivating mushroom is to adopt section wood or log to carry out outdoor manual dibbling's cultivation, and the woods wasting of resources is large, and the production cycle is subject to Effect of Natural Disaster, yields poorly, and owing to being subject to the restriction in trees, area, season, development speed is very slow.
The tame process of edible mushroom, be exactly in fact create artificially mushroom mycelium and fruit body development favourable, and process to other varied bacteria growing hostile environment conditions.Mushroom substituting stuff cultivation comes from this reason, and substituting stuff cultivation, for development Lentnus edodes, has been opened up a new way, is developed rapidly.So-called substituting stuff cultivation, utilizing exactly various agriculture and forestry accessory substances is primary raw material, adds appropriate auxiliary material, makes medium, replaces traditional planting material (log, Duan Mu) to produce various edible mushrooms.
Although in generation, expects that Lentnus edodes technology has developed for many years; and comparatively universal; but the technology that really can accomplish scale production is still immature at present; living contaminants, misshapen mushroom, fruiting are irregular etc., and phenomenon often occurs; have a strong impact on the quality of mushroom; in the cultivation production process of mushroom; living contaminants is the sixty-four dollar question facing; therefore; the sterilizing of mushroom culture medium requires very strict, and the inoculation after sterilizing requires to operate under aseptic condition especially, and such one is to have increased to become to produce cost; the 2nd, inoculation efficiency is reduced, the raising of restriction mushroom production.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of generation to expect the inoculation method of cultivating champignon, the method is without inoculating in gnotobasis, and inoculation efficiency is high, and the bactericidal agent environmental protection of using, harmless to health.
For realizing above-mentioned technical purpose, technical scheme of the present invention is:
Mushroom inoculation thimerosal, the ethanol by 95%, 50% carbendazim and strong chlorine oil form, and the proportioning of 95% ethanol, 50% carbendazim and strong chlorine oil is 500 ± 20ml:30 ± 5g:20 ± 2g.
Further, described mushroom inoculation thimerosal, the proportioning of 95% ethanol, 50% carbendazim and strong chlorine oil is 500ml:30g:20g.
In generation, is expected the inoculation method of cultivating champignon, comprises the step of carrying out as follows:
(1) pack the composts or fertilisers of cultivating of mushroom culture into plastic sack, tighten sack, must cultivation bag be inoculated after sterilizing is cooling, with Polypropylence Sheet will described in cultivation bag to be inoculated, mushroom strain and inoculating tool together seal, then according to the aerial fog disinfectant of every cube of 20 ± 2g, light sterilizing at least 5 hours;
(2) get 95% ethanol, 50% carbendazim and strong chlorine oil, the ethanol 500 ± 20ml:50% carbendazim 30 ± 5g according to 95%: the proportioning preparation thimerosal of strong chlorine oil 20 ± 2g is stand-by;
(3) after the middle aerial fog disinfectant sterilizing of step (1), open one end of described Polypropylence Sheet, cultivation bag to be inoculated described in taking-up, in cultivation bag appearance to be inoculated, coat the thimerosal sterilization of preparing in step (2), get the inoculating tool after sterilizing in step (1), in cultivation bag appearance to be inoculated, coated the position punching of thimerosal, bacterial classification is inoculated in hand-hole and must be inoculated cultivation bag, to inoculate cultivation bag stacking, length and width are no more than 2 meters, height of embankment is no more than 1.2 meters, then with Polypropylence Sheet, the inoculation cultivation bag of stacking is sealed to compacting, carries out bacteria backlight.
Further, described inoculation method, in described step (1), described aerial fog disinfectant is lighted sterilizing 5 hours.
Further, described inoculation method, in described step (3), the thimerosal sterilization of preparing in cultivation bag appearance to be inoculated is coated step (2) according to the width of 6 centimeters of every row, 3-5 the hole of punching, position of having coated thimerosal in cultivation bag appearance to be inoculated, bore dia is 1.5 ± 0.1cm.
Further, described inoculation method in described step (3), is inoculated bacterial classification in hand-hole under the condition of 5-20 ℃.
Beneficial effect of the present invention: generation of the present invention is expected the open inoculation method of cultivating champignon, the method is without completing the inoculation of mushroom by a lot of desinfection chamber of cost, and simple to operate, inoculation efficiency is high; Method of the present invention, thimerosal environmental protection is used in the mushroom inoculation of use, harmless to health.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearer, below the preferred embodiments of the present invention are described in detail.
Aerial fog disinfectant of the present invention is the disinfectant that is exclusively used in edible mushroom, as the explanation referring in http://web.zgny.com.cn/User_Doc/451/Products/6983/2011549335385 914511.htm.
Carbendazim has another name called carbendazol, carbendazol.Carbendazim is a kind of broad-spectrum germicide, and the disease that various crop is caused by fungi (as imperfect fungus, many sac fungis) has control efficiency.Can be used for foliar spray, seed treatment and soil treatment etc.In the present invention, 50% carbendazim is 50% carbendazol wettable powder, and its properties of product can be the introduction in http://www.docin.com/p-32157886.html referring to network address.
Strong chlorine oil is called TCCA (Trichloroisocyanuric acid), claim again TCCA, fish peace etc., it is efficient oxidation type Biocidal algae-killing agent, product is white crystalline powder, fusing point 225-230 ℃, fusing point adds thermolysis above, is slightly soluble in water, solvability in water (25 ℃ time) is 1.2g/100g water, is soluble in acetone and aqueous slkali.After dissolving in water, be hydrolyzed to hypochlorous acid and the acid of cyanogen urea, non-secondary pollution, is a kind of efficient, safe sterilizing and bleaching agent.
Embodiment is for the inoculation method of material cultivating champignon
Mushroom has lignin, cellulosic ability in the timber of decomposition, can be glucose, amino acid etc. by its decomposition and inversion, the nutriment that directly absorbs and utilize as hyphal cell.
Charge ratio preparation cultivation composts or fertilisers of cultivating according to weed tree sawdust 50%, milled powders of cornstalk 27%, wheat bran 20%, gypsum 2%, sugar 1%, then adds water-wet, to composts or fertilisers of cultivating water content, is that 50-60% is advisable.Select long 45-50cm, polyacrylic film that diameter is 5cm is made bacterium cylinder, and the composts or fertilisers of cultivating preparing is packed in plastic sack, tightens sack, the high temperature of 130-150 ℃ then, pressure 1.5-2ks/c, sterilizing 6 hours.
Mushroom inoculation thimerosal, the ethanol by 95%, 50% carbendazim and strong chlorine oil form, and the proportioning of 95% ethanol, 50% carbendazim and strong chlorine oil is 500 ± 20ml:30 ± 5g:20 ± 2g, prepares on request thimerosal standby.
Plastics bacterium cylinder by sterilizing after 6 hours takes out, must cultivation bag be inoculated after cooling, with Polypropylence Sheet will described in cultivation bag to be inoculated, mushroom strain and inoculating tool together seal, then according to the aerial fog disinfectant of every cube of 20 ± 2g, light sterilizing at least 5 hours, after aerial fog disinfectant sterilizing, open one end of described Polypropylence Sheet, cultivation bag to be inoculated described in taking-up, in cultivation bag appearance to be inoculated, coating above-mentioned prepared thimerosal carries out disinfection, according to the width of 6 centimeters of every row, be coated with thimerosal, then get the inoculating tool after sterilizing, in cultivation bag appearance to be inoculated, coated 3-5 the hole of punching, position of thimerosal, bore dia is 1.5 ± 0.1cm, under 16 ℃ of conditions, bacterial classification is inoculated in hand-hole and must be inoculated cultivation bag, to inoculate cultivation bag stacking, length and width are no more than 2 meters, height of embankment is no more than 1.2 meters, then with Polypropylence Sheet, the inoculation cultivation bag of stacking is sealed to compacting, carry out bacteria backlight, bacteria backlight after 25 days turning check living contaminants situation and carry out frame " well " font bacteria and process.
The result of inoculating by the method shows, within 25 days, there is not the situation of living contaminants in bacteria backlight, and mushroom mycelium length is good and fast, about 25 days, can send out purseful material, be the 8-10 of tradition inoculation efficiency in desinfection chamber doubly, and by the method, inoculation personnel can not be subject to the hardship of poisoning.
Finally explanation is, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (6)
1. mushroom inoculation thimerosal, is characterized in that, the ethanol by 95%, 50% carbendazim and strong chlorine oil form, and the proportioning of 95% ethanol, 50% carbendazim and strong chlorine oil is 500 ± 20ml:30 ± 5g:20 ± 2g.
2. mushroom inoculation thimerosal according to claim 1, is characterized in that, the proportioning of described 95% ethanol, 50% carbendazim and strong chlorine oil is 500ml:30g:20g.
3. in generation, is expected the open inoculation method of cultivating champignon, it is characterized in that, comprises the step of carrying out as follows:
(1) pack the composts or fertilisers of cultivating of mushroom culture into plastic sack, tighten sack, must cultivation bag be inoculated after sterilizing is cooling, with Polypropylence Sheet will described in cultivation bag to be inoculated, mushroom strain and inoculating tool together seal, then according to the aerial fog disinfectant of every cube of 20 ± 2g, light sterilizing at least 5 hours;
(2) get 95% ethanol, 50% carbendazim and strong chlorine oil, the ethanol 500 ± 20ml:50% carbendazim 30 ± 5g according to 95%: the proportioning preparation thimerosal of strong chlorine oil 20 ± 2g is stand-by;
(3) after the middle aerial fog disinfectant sterilizing of step (1), open one end of described Polypropylence Sheet, cultivation bag to be inoculated described in taking-up, in cultivation bag appearance to be inoculated, coat the thimerosal sterilization of preparing in step (2), get the inoculating tool after sterilizing in step (1), in cultivation bag appearance to be inoculated, coated the position punching of thimerosal, bacterial classification is inoculated in hand-hole and must be inoculated cultivation bag, to inoculate cultivation bag stacking, length and width are no more than 2 meters, height of embankment is no more than 1.2 meters, then with Polypropylence Sheet, the inoculation cultivation bag of stacking is sealed to compacting, carries out bacteria backlight.
4. in generation according to claim 3, is expected the open inoculation method of cultivating champignon, it is characterized in that, in described step (1), described aerial fog disinfectant is lighted sterilizing 5 hours.
5. in generation according to claim 3, is expected the open inoculation method of cultivating champignon, it is characterized in that, in described step (3), the thimerosal sterilization of preparing in cultivation bag appearance to be inoculated is coated step (2) according to the width of 6 centimeters of every row, 3-5 the hole of punching, position of having coated thimerosal in cultivation bag appearance to be inoculated, bore dia is 1.5 ± 0.1cm.
6. in generation according to claim 3, is expected the open inoculation method of cultivating champignon, it is characterized in that, in described step (3), bacterial classification is inoculated in hand-hole under the condition of 5-20 ℃.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009944A (en) * | 2015-08-03 | 2015-11-04 | 廊坊市森淼春食用菌有限公司 | Fast inoculation method for mushroom stick |
| CN106134755A (en) * | 2015-03-20 | 2016-11-23 | 陕西宁陕县秦南菌业科技有限公司 | A kind of open edible inoculation method |
| CN112352626A (en) * | 2020-12-11 | 2021-02-12 | 广西禾美生态农业股份有限公司 | Preparation method of shiitake mushroom sticks |
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| US3335521A (en) * | 1964-08-08 | 1967-08-15 | Sohm Elmar | Method for cultivation of champignons |
| CN101580424A (en) * | 2008-05-18 | 2009-11-18 | 李畅 | Preparation method of mushroom culture material |
| CN103168616A (en) * | 2012-10-29 | 2013-06-26 | 上海雪榕生物科技股份有限公司 | Technology of reducing pollution of cultivation bottle |
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| US3335521A (en) * | 1964-08-08 | 1967-08-15 | Sohm Elmar | Method for cultivation of champignons |
| CN101580424A (en) * | 2008-05-18 | 2009-11-18 | 李畅 | Preparation method of mushroom culture material |
| CN103168616A (en) * | 2012-10-29 | 2013-06-26 | 上海雪榕生物科技股份有限公司 | Technology of reducing pollution of cultivation bottle |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134755A (en) * | 2015-03-20 | 2016-11-23 | 陕西宁陕县秦南菌业科技有限公司 | A kind of open edible inoculation method |
| CN105009944A (en) * | 2015-08-03 | 2015-11-04 | 廊坊市森淼春食用菌有限公司 | Fast inoculation method for mushroom stick |
| CN112352626A (en) * | 2020-12-11 | 2021-02-12 | 广西禾美生态农业股份有限公司 | Preparation method of shiitake mushroom sticks |
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Effective date of registration: 20190428 Address after: 750002 Room 1004, Building 15, Wanhe City, Washington, 561 Ning'an Avenue, Jinfeng District, Yinchuan City, Ningxia Hui Autonomous Region Patentee after: Ningxia Qingyuan modern agricultural science and Technology Co., Ltd. Address before: 405803 No. 125 Xinjie, Wenfeng Town, Wuxi County, Chongqing Patentee before: Chongqing Fuxinyang Edible Fungus Co.,Ltd. |
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