CN112352626A - Preparation method of shiitake mushroom sticks - Google Patents
Preparation method of shiitake mushroom sticks Download PDFInfo
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- CN112352626A CN112352626A CN202011459224.0A CN202011459224A CN112352626A CN 112352626 A CN112352626 A CN 112352626A CN 202011459224 A CN202011459224 A CN 202011459224A CN 112352626 A CN112352626 A CN 112352626A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
- A01G18/55—Forming inoculation holes
Abstract
The invention relates to the technical field of edible mushroom production, in particular to a preparation method of a mushroom stick. The preparation method of the mushroom stick comprises the following steps of (1) culture material preparation, (2) mixing, (3) bagging and (4) sterilization. The mushroom stick solves the problem of environmental pollution caused by improper treatment of food processing leftovers, changes waste into valuable, has high economic benefit, does not add any harmful substance from the formula to the production process, is scientific and reasonable, has a simple preparation method, can provide sufficient and reasonable nutrients for the growth of mushrooms, can shorten the fungus growing period, and improves the yield.
Description
[ technical field ] A method for producing a semiconductor device
The invention belongs to the technical field of edible mushroom production, and particularly relates to a preparation method of a mushroom stick.
[ background of the invention ]
The edible fungi refers to large-scale fungi with large fruiting bodies and edible availability, and is commonly called mushroom. More than 350 kinds of edible fungi are known in China, wherein more of the edible fungi belong to the subphylum basidiomycotina, and the common edible fungi comprise: lentinus Edodes, straw mushroom, Agaricus campestris, Auricularia, Tremella, Hericium Erinaceus, caulis Bambusae in Taeniam, Tricholoma matsutake, Lentinus Edodes, Ganoderma, Cordyceps, truffle, herba Epimedii, and Boletus; a few belong to the subdivision ascomycotina, among which are: morchella, saddle fungus, truffle, etc.
In the prior art, the production of the mushroom is to adopt cottonseed hulls, mixed sawdust and other crop straws such as straws, wheat straws, corn stalks, bean stalks, corn cobs and the like, and to match livestock and poultry manure such as cow manure, horse manure, pig manure, chicken manure and the like as main raw materials to prepare a material of a mushroom stick, and then to sterilize the mushroom stick, inoculate strains, cultivate hyphae, manage fruiting and harvest the mushroom.
However, the existing shiitake stick cultivation has the following defects: 1. the culture medium is not sterilized in place, so that the growth of the cultivated edible fungi is influenced by thermophilic acid-resistant bacillus and other sundry fungi in the culture medium, the yield of the cultivated edible fungi is not high, and the economic benefit of the cultivated edible fungi cannot be achieved; 2. the method for sterilizing the fungus sticks is to treat the fungus sticks for 12 to 16 hours at 100 ℃, so that the time and the energy are consumed, and the situation of bag expansion and bag breakage can occur; 3. the mushroom sticks have insufficient nutrients, and the yield, the quality and the like of mushrooms are limited.
[ summary of the invention ]
In view of the above, the invention aims to provide a preparation method of a mushroom stick, the mushroom stick solves the problem of environmental pollution caused by improper treatment of food processing leftovers, changes waste into valuable, and has high economic benefit.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of a mushroom stick comprises the following steps:
(1) preparing a culture material: preparing a culture material for a fungus stick according to the following weight parts: 10-15 parts of wheat bran, 1-2 parts of peptide powder, 20-28 parts of sawdust, 3-5 parts of diethyl aminoethyl hexanoate, 1-2 parts of iodine powder, 6-12 parts of pomegranate seed residue, 2-3 parts of gypsum, 5-10 parts of shiitake yield increasing agent, 2-4 parts of compound sodium nitrophenolate, 1-2 parts of yeast and 1-3 parts of micro-capsule phycoerythrin;
(2) mixing materials: uniformly mixing the wheat bran, the peptide powder, the wood chips, the iodine powder, the pomegranate seed residue and the gypsum by adopting a mechanical stirring mode, then adding diethyl aminoethyl hexanoate, the shiitake yield increasing agent, the compound sodium nitrophenolate and the microcystin, fully and uniformly stirring, then mixing the materials, putting the materials into a sterilization tank, sterilizing for 1-2 hours at the temperature of 120-140 ℃ and the pressure of 1-2MPa, then reducing the pressure to 15-25 ℃, then adding yeast, uniformly mixing, fermenting for 3-5 days at the temperature of 40-45 ℃, taking out the fermented material, standing for 15-20 hours at the temperature of 65-70 ℃, and then adjusting the pH of the mixture to 7-8.5 to obtain a mixed matrix for later use;
(3) bagging: putting the mixed matrix into a plastic bag, and fastening the opening of the bag to prepare a material rod for later use;
(4) and (3) sterilization: and (3) coating disinfecting liquid on the surface of the material stick for disinfection, punching a hole at the position to be inoculated with the disinfecting liquid on the surface of the material stick, and inoculating strains into the hole to obtain the mushroom stick.
The Lentinus edodes stick can be used for cultivating edible fungi (Lentinus edodes), and is prepared by stacking Lentinus edodes stick with length and width not more than 2m and stack height not more than 1.2 m, sealing and compacting the stacked inoculated cultivation bag with plastic cloth, and culturing fungi in backlight.
In the invention, the shiitake fungus stick is further composed of the following raw materials in parts by weight: 12 parts of wheat bran, 2 parts of peptide powder, 24 parts of sawdust, 4 parts of diethyl aminoethyl hexanoate, 2 parts of iodine powder, 9 parts of pomegranate seed residue, 2 parts of gypsum, 7 parts of mushroom yield increasing element, 3 parts of compound sodium nitrophenolate, 2 parts of yeast and 2 parts of microcystin.
In the invention, the disinfectant is further composed of the following raw materials in parts by weight: 8-12 parts of ethanol, 3-5 parts of micracacin, 1-2 parts of fungistatin, 0.5-1.5 parts of active agent, 3-5 parts of hymexazol and 20-30 parts of water.
In the invention, further, the pomegranate seed residue is obtained by the following method: a. crushing pomegranate seeds, adding guanidine salt ionic liquid into the crushed pomegranate seeds, stirring for 40-45 minutes, then adding water with the volume 2-4 times that of the material obtained after stirring, stirring for 40-50 minutes again, centrifuging, collecting precipitate, drying the precipitate in vacuum to obtain pomegranate seed powder, adding water with the mass 3-5 times that of the pomegranate seed powder, uniformly mixing, adding cellulase into the mixture, and performing enzymolysis for 6-8 hours to obtain the pomegranate seed residue.
In the invention, further, the conditions of the cellulase hydrolysis are as follows: the pH value is 6-6.5, and the temperature is 55-65 ℃.
In the invention, furthermore, the rotation speed of the two stirring treatments is 85-100r/min, and the temperature of the stirring treatment is 100-105 ℃.
The invention has at least the following beneficial effects:
1. the mushroom stick solves the problem of environmental pollution caused by improper treatment of food processing leftovers, changes waste into valuable, has high economic benefit, does not add any harmful substance from the formula to the production process, is scientific and reasonable, has a simple preparation method, can provide sufficient and reasonable nutrients for the growth of mushrooms, can shorten the fungus growing period, and improves the yield.
2. In the edible fungus cultivation raw material, the wheat bran can provide sufficient protein, the pomegranate seed residue is obtained by hydrolysis modification treatment, the pomegranate seed residue is regenerated by adopting the ionic liquid, then hydrolyzing with cellulase, wherein the pomegranate seed residue mainly contains cellulose, reducing sugar and other components, the dissolved cellulose solution can be precipitated after adding water to realize cellulose regeneration, lignin can be removed in the regeneration process, the inhibition effect on the subsequent enzymatic hydrolysis process is reduced, meanwhile, the regeneration process can lead partial hydrogen bonds in cellulose molecules to be broken, the degree of polymerization and the degree of crystallinity to be reduced, the surface morphology of the cellulose is changed, the surface area is increased, and the contact area with enzyme is increased, the utilization of pomegranate seed residues as the culture medium reduces the consumption of the raw materials of the culture medium for providing a carbon source in the traditional process, and saves the preparation cost of the mushroom sticks; the microcystin is rich in active oxygen, the low-concentration active oxygen in the edible fungi can conduct multiple response reactions in the cell signal transduction path of the edible fungi as a second messenger, the compound sodium nitrophenolate can quickly permeate into the edible fungi after being contacted with the edible fungi to promote the protoplasm flow of the cells and improve the cell activity, the microcystin and the compound sodium nitrophenolate are used in combination, the microcystin can also quickly permeate into the edible fungi under the driving of the compound sodium nitrophenolate, in addition, the diethyl aminoethyl hexanoate can improve the activities of peroxidase and nitrate reductase of the edible fungi to promote the development of the edible fungi and adjust the balance of nutrients in the bodies, the added wood chips are crude fiber biomass, the insoluble macromolecular compounds contained in the cultivation raw materials are decomposed into simple soluble substances by mycelia, the nutrients absorbed and utilized by the edible fungi can be effectively improved, and the wood chips have loose porous performance and can increase the content of organic matters, promoting the formation and transformation of humus and granular groups, improving the water retention performance and fertility of the cultivation base material, and uniformly dispersing the fertilizer efficiency.
3. The invention changes the traditional sterilization mode, and has short sterilization time and good effect by carrying out high-temperature and high-pressure sterilization in the sterilization tank, then decompression cooling is carried out, yeast is added for fermentation, the fermented fungus stick material is kept at medium temperature for a period of time, the nutrients of the fungus stick can be excited, the growth environment of the fungus stick is adjusted to a state which is most beneficial to the fungus growing culture of the fungus stick in the later period, after preparing the fungus stick, punching a hole at the position of the outer surface of the material stick to be inoculated, which is coated with the disinfectant, inoculating the fungus strain into the hole, avoids the pollution of mixed fungi, has good inoculation effect, can effectively inhibit the normal growth of pathogenic fungi mycelium or directly kill pathogenic fungi by compounding and adding hymexazol in the disinfectant, can promote the growth of edible fungi, the permeability of the mushroom culture medium is extremely high, so that the mushroom culture medium is guaranteed to have a healthy culture environment at the first time, and the survival rate of the mushroom is improved; the mushroom stick is an open type inoculation method, inoculation of mushrooms can be completed without a sterile room which is quite expensive, the operation is simple, and the inoculation efficiency is high.
[ detailed description ] embodiments
The following examples may help one skilled in the art to more fully understand the present invention, but are not intended to limit the invention in any way.
Example 1:
the embodiment provides a preparation method of a shiitake mushroom stick, which comprises the following steps:
(1) preparing a culture material: preparing a culture material for a fungus stick according to the following weight parts: 10 parts of wheat bran, 1 part of peptide powder, 20 parts of sawdust, 3 parts of diethyl aminoethyl hexanoate, 6 parts of iodine powder, 6 parts of pomegranate seed residue, 2 parts of gypsum, 5 parts of shiitake yield increasing element, 2 parts of compound sodium nitrophenolate, 1 part of yeast and 1 part of microcystin; the pomegranate seed residue is obtained by the following method: a. crushing pomegranate seeds, adding guanidine salt ionic liquid into the crushed pomegranate seeds, stirring for 40 minutes, then adding water with the volume 2 times that of the material obtained after stirring, stirring for 40 minutes again, centrifuging to collect precipitate, drying the precipitate in vacuum to obtain pomegranate seed powder, adding water with the mass 3 times that of the pomegranate seed powder into the pomegranate seed powder, uniformly mixing, and adding cellulase into the mixture for enzymolysis for 6 hours to obtain pomegranate seed residues; the conditions of the cellulase hydrolysis are as follows: the pH value is 6, and the temperature is 55 ℃; the rotating speed of the two stirring treatments is 85r/min, and the temperature of the stirring treatment is 100 ℃;
(2) mixing materials: uniformly mixing the wheat bran, the peptide powder, the wood chips, the iodine powder, the pomegranate seed residues and the gypsum in a mechanical stirring manner, then adding diethyl aminoethyl hexanoate, the shiitake yield increasing agent, the compound sodium nitrophenolate and the microcystin, fully stirring, then mixing the materials, putting the materials into a sterilization tank, sterilizing for 1 hour at the temperature of 120 ℃ and the pressure of 1MPa, then reducing the pressure to 15 ℃, then adding yeast, uniformly mixing, fermenting for 3 days at the temperature of 40 ℃, taking out the fermented material, standing for 15 hours at the temperature of 65 ℃, and then adjusting the pH value of the mixture to 7 to obtain a mixed matrix for later use;
(3) bagging: putting the mixed matrix into a plastic bag, and fastening the opening of the bag to prepare a material rod for later use;
(4) and (3) sterilization: coating disinfecting liquid on the surface of the material stick for disinfection, punching a hole at the position of the material stick to be inoculated, which is coated with the disinfecting liquid, and inoculating strains into the hole to obtain the mushroom stick; the disinfectant consists of the following raw materials in parts by weight: 8 parts of ethanol, 3 parts of micracacin, 1 part of fungistatin, 0.5 part of active agent, 3 parts of hymexazol and 20 parts of water.
Example 2:
(1) preparing a culture material: preparing a culture material for a fungus stick according to the following weight parts: 12 parts of wheat bran, 2 parts of peptide powder, 24 parts of sawdust, 4 parts of diethyl aminoethyl hexanoate, 2 parts of iodine powder, 9 parts of pomegranate seed residue, 2 parts of gypsum, 7 parts of shiitake yield increasing element, 3 parts of compound sodium nitrophenolate, 2 parts of yeast and 2 parts of microcystin; the pomegranate seed residue is obtained by the following method: a. crushing pomegranate seeds, adding guanidine salt ionic liquid into the crushed pomegranate seeds, stirring for 42 minutes, then adding water with the volume 3 times that of the material obtained after stirring, stirring for 45 minutes again, centrifuging to collect precipitate, drying the precipitate in vacuum to obtain pomegranate seed powder, adding water with the mass 4 times that of the pomegranate seed powder into the pomegranate seed powder, uniformly mixing, and adding cellulase into the mixture for enzymolysis for 7 hours to obtain pomegranate seed residues; the conditions of the cellulase hydrolysis are as follows: the pH value is 6.2, and the temperature is 60 ℃; the rotating speed of the two times of stirring treatment is 92r/min, and the temperature of the stirring treatment is 103 ℃;
(2) mixing materials: uniformly mixing the wheat bran, the peptide powder, the wood chips, the iodine powder, the pomegranate seed residue and the gypsum by adopting a mechanical stirring mode, then adding diethyl aminoethyl hexanoate, the shiitake yield increasing agent, the compound sodium nitrophenolate and the microcystin, fully stirring, then mixing the materials, putting the materials into a sterilization tank, sterilizing for 1.5 hours at the temperature of 130 ℃ and the pressure of 1.5MPa, then reducing the pressure to 20 ℃, then adding yeast, uniformly mixing, fermenting for 4 days at the temperature of 42 ℃, taking out the fermented material, standing for 17 hours at the temperature of 67 ℃, and then adjusting the pH of the mixture to 7.8 to obtain a mixed matrix for later use;
(3) bagging: putting the mixed matrix into a plastic bag, and fastening the opening of the bag to prepare a material rod for later use;
(4) and (3) sterilization: coating disinfecting liquid on the surface of the material stick for disinfection, punching a hole at the position of the material stick to be inoculated, which is coated with the disinfecting liquid, and inoculating strains into the hole to obtain the mushroom stick; the disinfectant consists of the following raw materials in parts by weight: 10 parts of ethanol, 4 parts of micracacin, 1 part of fungistatin, 1 part of active agent, 4 parts of hymexazol and 25 parts of water.
Example 3:
(1) preparing a culture material: preparing a culture material for a fungus stick according to the following weight parts: 15 parts of wheat bran, 2 parts of peptide powder, 28 parts of sawdust, 5 parts of diethyl aminoethyl hexanoate, 2 parts of iodine powder, 12 parts of pomegranate seed residue, 3 parts of gypsum, 10 parts of shiitake yield increasing element, 4 parts of compound sodium nitrophenolate, 2 parts of yeast and 3 parts of microcystin; the pomegranate seed residue is obtained by the following method: a. crushing pomegranate seeds, adding guanidine salt ionic liquid into the crushed pomegranate seeds, stirring for 45 minutes, then adding water with the volume 4 times that of the material obtained after stirring, stirring for 50 minutes again, centrifuging to collect precipitate, drying the precipitate in vacuum to obtain pomegranate seed powder, adding water with the mass 5 times that of the pomegranate seed powder into the pomegranate seed powder, uniformly mixing, and adding cellulase into the mixture for enzymolysis for 8 hours to obtain pomegranate seed residues; the conditions of the cellulase hydrolysis are as follows: the pH value is 6.5, and the temperature is 65 ℃; the rotating speed of the two stirring treatments is 100r/min, and the temperature of the stirring treatment is 105 ℃;
(2) mixing materials: uniformly mixing the wheat bran, the peptide powder, the wood chips, the iodine powder, the pomegranate seed residues and the gypsum by adopting a mechanical stirring mode, then adding diethyl aminoethyl hexanoate, the shiitake yield increasing agent, the compound sodium nitrophenolate and the microcystin, fully stirring, then mixing the materials, putting the materials into a sterilization tank, sterilizing for 2 hours at the temperature of 140 ℃ and the pressure of 2MPa, then reducing the pressure to 25 ℃, then adding yeast, uniformly mixing, fermenting for 5 days at the temperature of 45 ℃, taking out the fermented material, standing for 20 hours at the temperature of 70 ℃, and then adjusting the pH of the mixture to 8.5 to obtain a mixed matrix for later use;
(3) bagging: putting the mixed matrix into a plastic bag, and fastening the opening of the bag to prepare a material rod for later use;
(4) and (3) sterilization: coating disinfecting liquid on the surface of the material stick for disinfection, punching a hole at the position of the material stick to be inoculated, which is coated with the disinfecting liquid, and inoculating strains into the hole to obtain the mushroom stick; the disinfectant consists of the following raw materials in parts by weight: 12 parts of ethanol, 5 parts of micracacin, 2 parts of fungistatin, 1.5 parts of active agent, 5 parts of hymexazol and 30 parts of water.
Effect verification
To illustrate the utility value of the present application, the applicant conducted the following tests, respectively:
a first group: the compound sodium nitrophenolate is removed, and the other modes are strictly carried out according to the embodiment 2;
second group: the diethyl aminoethyl hexanoate was removed and the procedure was otherwise exactly as in example 2;
third group: the microcystin is removed and the other way is strictly performed according to the example 2;
and a fourth group: the step of preparing the pomegranate seed residue is removed, and the other modes are strictly carried out according to the embodiment 2;
and a fifth group: the disinfectant is removed, and the other way is strictly carried out according to the example 2;
a sixth group: the hymexazol in the disinfectant is removed, and the other modes are strictly carried out according to the embodiment 2;
a seventh group: the disinfectant is prepared by mixing ethanol, carbendazim and chloroquine according to the mass ratio of 5:2:1, and the other modes are strictly carried out according to the embodiment 2;
and an eighth group: the sterilization mode is changed to: sterilizing at 100 deg.C for 12-16 hr, otherwise strictly according to example 2;
ninth group: the step of reducing the temperature under reduced pressure after sterilization is removed, the temperature is directly and naturally reduced to the required temperature (20 ℃), and the other modes are strictly carried out according to the embodiment 2;
the tenth group: the step of reducing the pressure and the temperature after sterilization is removed, the operation of adding the yeast for fermentation is directly carried out, and the other modes are strictly carried out according to the embodiment 2;
eleventh group: yeast and yeast fermentation are removed, and other modes are strictly carried out according to the embodiment 2;
a twelfth group: the sterilization mode is changed to: sterilizing at 80 ℃ for 4 hours, and performing other modes strictly according to the embodiment 2;
group thirteen: the mushroom stick of embodiment 2 of this application.
Each of the thirteen groups comprises 30 bags of mushroom sticks, the mushroom sticks are long sticks with the size of 15cm x 55cm x 0.005cm, strains with the mass of 0.6% of the mushroom sticks are inoculated after the mushroom sticks are obtained, then the mushroom sticks are stacked, the length and the width are not more than 2 meters, the stack height is not more than 1.2 meters, then the stacked inoculation cultivation bags are sealed and compacted by plastic cloth, and the backlight cultivation is carried out.
Comparing the cultivation effect of the mushroom sticks obtained by the different methods, the recorded data are shown in table 1:
TABLE 1 contrast of fruiting effect of mushroom stick
According to the table, the mushroom stick can provide sufficient and reasonable nutrients for the growth of mushrooms, shorten the spawn running period, improve the yield and the quality, and has obvious advantages compared with other groups.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (6)
1. A preparation method of a mushroom stick is characterized by comprising the following steps:
(1) preparing a culture material: preparing a culture material for a fungus stick according to the following weight parts: 10-15 parts of wheat bran, 1-2 parts of peptide powder, 20-28 parts of sawdust, 3-5 parts of diethyl aminoethyl hexanoate, 1-2 parts of iodine powder, 6-12 parts of pomegranate seed residue, 2-3 parts of gypsum, 5-10 parts of shiitake yield increasing agent, 2-4 parts of compound sodium nitrophenolate, 1-2 parts of yeast and 1-3 parts of micro-capsule phycoerythrin;
(2) mixing materials: uniformly mixing the wheat bran, the peptide powder, the wood chips, the iodine powder, the pomegranate seed residue and the gypsum by adopting a mechanical stirring mode, then adding diethyl aminoethyl hexanoate, the shiitake yield increasing agent, the compound sodium nitrophenolate and the microcystin, fully and uniformly stirring, then mixing the materials, placing the materials into a sterilization tank, sterilizing for 1-2 hours at the temperature of 120-140 ℃ and the pressure of 1-2MPa, then reducing the pressure to 15-25 ℃, then adding yeast, uniformly mixing, fermenting for 3-5 days at the temperature of 40-45 ℃, taking out the fermented material, placing the fermented material at the temperature of 65-70 ℃ for standing for 15-20 hours, and then adjusting the pH value of the mixture to 7-8.5 to obtain a mixed matrix for later use;
(3) bagging: putting the mixed matrix into a plastic bag, and fastening the opening of the bag to prepare a material rod for later use;
(4) and (3) sterilization: and (3) coating disinfecting liquid on the surface of the material stick for disinfection, punching a hole at the position to be inoculated with the disinfecting liquid on the surface of the material stick, and inoculating strains into the hole to obtain the mushroom stick.
2. The preparation method of the shiitake mushroom stick according to claim 1, wherein the shiitake mushroom stick is prepared from the following raw materials in parts by weight: 12 parts of wheat bran, 2 parts of peptide powder, 24 parts of sawdust, 4 parts of diethyl aminoethyl hexanoate, 2 parts of iodine powder, 9 parts of pomegranate seed residue, 2 parts of gypsum, 7 parts of mushroom yield increasing element, 3 parts of compound sodium nitrophenolate, 2 parts of yeast and 2 parts of microcystin.
3. The preparation method of the shiitake mushroom stick as claimed in claim 1, wherein the disinfectant is prepared from the following raw materials in parts by weight: 8-12 parts of ethanol, 3-5 parts of micracacin, 1-2 parts of fungistatin, 0.5-1.5 parts of active agent, 3-5 parts of hymexazol and 20-30 parts of water.
4. The preparation method of the shiitake mushroom stick according to claim 1, wherein the pomegranate seed residue is obtained by the following method: a. crushing pomegranate seeds, adding guanidine salt ionic liquid into the crushed pomegranate seeds, stirring for 40-45 minutes, then adding water with the volume 2-4 times that of the material obtained after stirring, stirring for 40-50 minutes again, centrifuging, collecting precipitate, drying the precipitate in vacuum to obtain pomegranate seed powder, adding water with the mass 3-5 times that of the pomegranate seed powder, uniformly mixing, adding cellulase into the mixture, and performing enzymolysis for 6-8 hours to obtain the pomegranate seed residue.
5. The method for preparing mushroom sticks according to claim 4, wherein the cellulose enzymolysis conditions are as follows: the pH value is 6-6.5, and the temperature is 55-65 ℃.
6. The method for preparing mushroom stick as claimed in claim 4, wherein the rotation speed of the two stirring processes is 85-100r/min, and the temperature of the stirring process is 100-105 ℃.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688749A (en) * | 2013-12-12 | 2014-04-02 | 重庆市福鑫洋食用菌有限公司 | Open type inoculation method for cultivating mushroom with substitute material |
| CN104945169A (en) * | 2015-07-15 | 2015-09-30 | 龚灿锋 | Compound fertilizer for crops |
| CN104956918A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Method for preparing mushroom sticks |
| CN108293877A (en) * | 2018-02-11 | 2018-07-20 | 平南县德湖种养农民专业合作社 | A kind of Monstera deliciosa seed tissue culture medium |
| CN111357568A (en) * | 2020-04-28 | 2020-07-03 | 广西壮族自治区农业科学院 | Edible fungus cultivation base material and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103688749A (en) * | 2013-12-12 | 2014-04-02 | 重庆市福鑫洋食用菌有限公司 | Open type inoculation method for cultivating mushroom with substitute material |
| CN104956918A (en) * | 2015-07-08 | 2015-10-07 | 翔天农业开发集团股份有限公司 | Method for preparing mushroom sticks |
| CN104945169A (en) * | 2015-07-15 | 2015-09-30 | 龚灿锋 | Compound fertilizer for crops |
| CN108293877A (en) * | 2018-02-11 | 2018-07-20 | 平南县德湖种养农民专业合作社 | A kind of Monstera deliciosa seed tissue culture medium |
| CN111357568A (en) * | 2020-04-28 | 2020-07-03 | 广西壮族自治区农业科学院 | Edible fungus cultivation base material and preparation method thereof |
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Application publication date: 20210212 |