CN107896818A - A kind of cultural method of agaricus bisporus - Google Patents
A kind of cultural method of agaricus bisporus Download PDFInfo
- Publication number
- CN107896818A CN107896818A CN201711114617.6A CN201711114617A CN107896818A CN 107896818 A CN107896818 A CN 107896818A CN 201711114617 A CN201711114617 A CN 201711114617A CN 107896818 A CN107896818 A CN 107896818A
- Authority
- CN
- China
- Prior art keywords
- parts
- culture
- mushroom
- water
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The present invention relates to mushroom-cultivating field, specifically a kind of cultural method of sharp agaricus bisporus.The present invention passes through PERFORMANCE OF MODIFIED VERMICULITE, obtain expanded vermiculite, expanded vermiculite has very strong water imbibition, pass through the ability of the ion exchange of vermiculite, can be with loose earthing, beneficial to the formation of agaricus bisporus fruit body primordium, improve poor aeration because cause mycelia because of anoxic and vigor decline the problem of, strengthen water holding capacity, add polyglutamic acid, pass through both mixing, it is laid between mycelia and earthing, during mycelial growth to overburden layer, by water-soluble, thin film can be formed on mycelia top layer, it can allow nutrient in earthing, moisture content is contacted with each other and conveyed with mycelia, can very it is efficient improve nutrient dissolving, storage, conveying and absorption, improve agaricus bisporus cultivating rate.
Description
Technical field
The present invention relates to mushroom-cultivating field, specifically a kind of cultural method of sharp agaricus bisporus.
Background technology
Soil is the base camp of microorganism, in soil containing microorganism growth metabolism in laboratory conditions lacked it is special because
Son, therefore, by the method for Culture in situ, can be such that " microorganism that cannot be cultivated " can also grows in laboratory conditions.Training
Support and soil water-soluble extractive is added in base, it is possible to increase the expression quantity of bacterium and actinomyces known, and activate silence
Gene.However, influence of the soil extraction to fungi, has not been reported.
Agaricus bisporus is the edible mushroom for cultivating most extensive, output and consumption figure maximum in the world, and yield accounts for world's food
With the 3/4 of bacterium total output.Common optimum medium for stock spawn of Agaricus bisporus is potato dextrose agar（PDA）With add richness
PDA.Cultigen has solid spawn and liquid spawn, and solid spawn culture medium is often with grain such as wheat or small rice grain, liquid spawn
Culture medium is common to be had plus rich potato dextrose medium（Add rich PD）, sucrose peptone culture medium and malt extract culture
Base.When preparing agaricus bisporus strain using these culture mediums, mycelial growth is slow, long potential difference, strain production cycle length.
Cultivation of agaricus bisporus material windrow is used as thing stalk（Wheat straw, rice straw or corn stalk）And feces of livestock and poultry（Chicken manure, cow dung
Or horsehit）Formed Deng through fermentation reactor system, Common Cultivation kind is for primary raw material with cotton seed hulls, wheat, sawdust etc..Fermentation reactor system mistake
Journey often undergoes secondary fermentation.Cultivating bisporous mushroom can be inoculated with by the windrow of secondary fermentation.Cultivation of agaricus bisporus material windrow
Rich in cellulose, hemicellulose, lignin, nitrogenous compound and mineral element, and the enzyme of Microbe synthesis and antibiotic etc..
But these culture materials but also respectively have shortcoming, pure kernel culture：Nutrition is excessively abundant, careless slightly to pollute, pollution rate one
As more than 10%；Wheat easily rises brokenly in autoclaving, pollutes rate increase；Consume a large amount of grains.Saw-dust：Wood
It is poor to consider material moisture retention to be worth doing, after planting the easy desiccation of strain, lose sprouting ability；Mycelia vigor is poor, the easy aging of strain.Cotton seed
Shell strain：Cotton seed hull is prewetted more time-consuming, easily occurs prewetting insufficient, so as to cause sterilizing to be not thorough；Cotton seed hull needs
The fermentation of long period is wanted, if fermentation is not thorough, mycelia not material feeding or slow-growing easily occurs；Southern cotton seed hull is in short supply, valency
Lattice are high.
Therefore, research, using agaricus bisporus soil covering fruiting mechanism so that cultivation when not earthing or using other replacement materials
Material, the cultivation production to agaricus bisporus or other edible mushrooms is extremely important, also very urgent.
The content of the invention
The technical problems to be solved by the invention：Slow, the poor water retention property for production of hybrid seeds speed in current water agaricus bisporus incubation,
Moisture is easy to run off providing a kind of cultural method of agaricus bisporus.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of cultural method of agaricus bisporus, the preparation method include the following steps：
（1）Agaricus bisporus mushroom is seeded in plating medium, is cultivated, White mushroom mycelia, picking activation button mushroom must be activated
Silk is seeded to level liquid bacterium culture medium culture, obtains first class inoculum, first class inoculum is seeded in solid pedigree seed culture medium and is trained
Support, after mycelia is covered with, all original seed mycelium inoculations of picking to solid are planted in culture medium Bag Material, an end closure, and culture, must plant
Bacterium mycelia, by plantation bacterium mycelium inoculation to second class inoculum fermentation medium culture, obtains button mushroom fermentation culture, by White mushroom
Bacterium fermentation culture, which is seeded in solid state cultivation kind culture medium, expands culture, obtains liquid button mushroom and cures culture；
（2）By vermiculite and water Hybrid Heating, vermiculite mixed solution is obtained, odium stearate, metatitanic acid four are added into vermiculite mixed solution
Isopropyl ester, stirring reaction, pulls vermiculite out, dry, obtains PERFORMANCE OF MODIFIED VERMICULITE, and polyglutamic acid is added into PERFORMANCE OF MODIFIED VERMICULITE and is uniformly mixed, powder
Broken sieving, obtains water conservation mixed-powder；
（3）Wheat straw is cultivated into decomposed material and piles material heap, heap overlying PVC plastic film, film ventilation is taken off every 24h, is passed through steam heating material
Heap, when material temperature rises to 50 ~ 53 DEG C, carries out turning, repeats turning 3 ~ 4 times, fermentation, obtains fermentation material heap, fermentation material is stowed
Enter polypropylene plastics pocket, liquid button mushroom curing culture is uniformly seeded to fermentation material heap surface, is cultivated；
（4）After culture, the material in polypropylene plastics is poured into plastic crate, first the material table in polypropylene plastics
Face uniformly spreads one layer of water conservation powder, repaves one layer of peat earthing, sprays water, and it is 70% peat earthing face is kept humidity, keeps sky
Gas relative humidity 80% ~ 90%, temperature control is at 15 ~ 20 DEG C, culture, up to agaricus bisporus.
The step（1）Middle plating medium：According to the mass fraction, 150 ~ 200 parts of potato is taken, 15 ~ 20 parts of sucrose,
KH2PO41 ~ 2 part, MgSO47H2O0.05 ~ 0.1 part, 15 ~ 20 parts of agar, 1000 parts of water, pH are natural；Level liquid Spawn incubation
Base：According to the mass fraction, brewer's wort 30m30 ~ 35 part, 8 ~ 10 parts of corn flour, KH are taken2PO40.8 ~ 1.2 part, MgSO40.3~0.5
Part, VB10.01 ~ 0.02 part, 1000 parts of water, pH6.5 ± 0.2；
Second class inoculum fermentation medium：According to the mass fraction, 18 ~ 20 parts of wheat bran is taken, 5 ~ 10 parts of corn flour, 1 ~ 3 part of peptone,
KH2PO40.6 ~ 0.8 part, MgSO40.3 ~ 0.5 part, VB10.01 ~ 0.02 part, 0.05 ~ 0.08 part of fresh food oil, 1000 parts of water,
pH6.5±0.2；Solid pedigree seed culture medium：According to the mass fraction, 900 ~ 1000 parts of wheat, 10 ~ 15 parts of excrement grass, lime 5 ~ 8 are taken
Part, 5 ~ 10 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2；Solid state cultivation kind culture medium：According to the mass fraction, wheat 800 ~ 900 is taken
Part, 80 ~ 90 parts of wheat bran, 7 ~ 10 parts of excrement grass, 10 ~ 12 parts of lime, 8 ~ 10 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2.
The step（1）Middle first class inoculum condition of culture is：23 ~ 25 DEG C, 5 ~ 6d of rotating speed 150r/min shaken cultivations；Poly- third
The specification of alkene cultigen bag is 12cm × l8cm × 0.005cm；The inoculum concentration for planting bacterium mycelia is that mass fraction is 5%；White mushroom
The condition of culture of bacterium fermentation culture is：Cultivation temperature is 23 ~ 25 DEG C, initial pH6.5,60 ~ 100r/min of rotating speed, ventilation quantity
1V/V/min, cultivates 5 ~ 6d；Button mushroom fermentation culture in mass ratio 1:10 are seeded to solid state cultivation kind culture；The double spores of liquid
Mushroom cures culture condition of culture as 23 ~ 25 DEG C of 5 ~ 7d of culture.
The step（2）Middle vermiculite and the mass ratio of deionized water are 1:8；In vermiculite mixed solution with odium stearate, titanium
The mass ratio of sour tetra-isopropyl is 9:2:3；The mass ratio of PERFORMANCE OF MODIFIED VERMICULITE and polyglutamic acid is 3:2.
The step（3）Middle wheat straw cultivates decomposed material：According to the mass fraction, 500 ~ 600 parts of wheat straw is taken, oxen and horses excrement 200 ~
250 parts, 50 ~ 70 parts of cake fertilizer, 3 ~ 5 parts of urea, 25 ~ 30 parts of gypsum, 10 ~ 15 parts of calcium superphosphate, 8 ~ 10 parts of lime, 1500 parts of water,
pH6.5±0.2。
The step（3）Middle fermentation condition is 50 ~ 53 DEG C, 13 ~ 15d of fermentation；By the inoculum concentration of mass fraction 10% by liquid
Button mushroom cures culture and is uniformly seeded to fermentation material heap surface, and condition of culture is to be cultivated 25 ~ 30 days at 20 ~ 25 DEG C.
The step（4）Middle water conservation layer thickness about 0.8 ~ 1cm, peat earthing layer thickness about 3 ~ 3.5cm.
Compared with other methods, advantageous effects are the present invention：
（1）Liquid spawn cell age is short, it is active it is big, sprout fast, penetration range is big after accessing compost, and germination point increases, can be very
It is colonized in short time in media surface so as to reduce pollution, while also accelerates mycelium and cover with cultivating bag speed, is shortened
The bacterium germination time and the negative effect that upper strata mycelia aging is brought is avoided, the liquid spawn of fermented and cultured is accessed into solid culture
Base, to obtain solid material cultigen, then accesses compost, and original seed can control kind of a time significantly, accelerates production of hybrid seeds speed, reduces system
Kind efficiency；
（2）The present invention obtains expanded vermiculite by PERFORMANCE OF MODIFIED VERMICULITE, and expanded vermiculite has very strong water imbibition, by vermiculite from
The ability that son exchanges, beneficial to the formation of agaricus bisporus fruit body primordium, can improve poor aeration because so that mycelia with loose earthing
The problem of vigor declines because of anoxic, strengthens water holding capacity, adds polyglutamic acid, by both mixing, is laid on mycelia with covering
Between soil, during mycelial growth to overburden layer, by water-soluble, thin film can be formed on mycelia top layer, can allow and cover
Nutrient, moisture content and mycelia in soil are contacted with each other and conveyed, can very efficient dissolving, storage, conveying and the suction for improving nutrient
Receive, improve agaricus bisporus cultivating rate.
Embodiment
Bacterial strain：Agaricus bisporus, is preserved in Hua Zhong Agriculture University's Culture Collection.
Plating medium：According to the mass fraction, 150 ~ 200 parts of potato, 15 ~ 20 parts of sucrose, KH are taken2PO41 ~ 2 part,
MgSO4·7H2O0.05 ~ 0.1 part, 15 ~ 20 parts of agar, 1000 parts of water, pH are natural.
Level liquid bacterium culture medium：According to the mass fraction, brewer's wort 30m30 ~ 35 part are taken, 8 ~ 10 parts of corn flour,
KH2PO40.8 ~ 1.2 part, MgSO40.3 ~ 0.5 part, VB10.01 ~ 0.02 part, 1000 parts of water, pH6.5 ± 0.2.
Second class inoculum fermentation medium：According to the mass fraction, 18 ~ 20 parts of wheat bran, 5 ~ 10 parts of corn flour, peptone 1 ~ 3 are taken
Part, KH2PO40.6 ~ 0.8 part, MgSO40.3 ~ 0.5 part, VB10.01 ~ 0.02 part, 0.05 ~ 0.08 part of fresh food oil, water 1000
Part, pH6.5 ± 0.2.
Solid pedigree seed culture medium：According to the mass fraction, 900 ~ 1000 parts of wheat, 10 ~ 15 parts of excrement grass, 5 ~ 8 parts of lime, stone are taken
5 ~ 10 parts of cream, 1500 parts of water, pH6.5 ± 0.2.
Solid state cultivation kind culture medium：According to the mass fraction, 800 ~ 900 parts of wheat is taken, 80 ~ 90 parts of wheat bran, 7 ~ 10 parts of excrement grass,
10 ~ 12 parts of lime, 8 ~ 10 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2.
Wheat straw cultivates decomposed material：According to the mass fraction, 500 ~ 600 parts of wheat straw, 200 ~ 250 parts of oxen and horses excrement, cake fertilizer 50 ~ 70 are taken
Part, 3 ~ 5 parts of urea, 25 ~ 30 parts of gypsum, 10 ~ 15 parts of calcium superphosphate, 8 ~ 10 parts of lime, 1500 parts of water, pH6.5 ± 0.2.
A kind of cultural method of agaricus bisporus, includes the following steps：
（1）Agaricus bisporus mushroom is seeded in plating medium, 23 ~ 25 DEG C of 5 ~ 7d of culture, must activate White mushroom mycelia, picking is lived
Change White mushroom mycelia and be seeded to level liquid bacterium culture medium culture, 23 ~ 25 DEG C, rotating speed 150r/min 5 ~ 6d of shaken cultivation, obtain
First class inoculum, first class inoculum is seeded in solid pedigree seed culture medium and is cultivated, after mycelia is covered with, all original seed mycelium inoculations of picking
To be planted to solid in culture medium Bag Material, the specification of polypropylene cultigen bag is 12cm × l8cm × 0.005cm, an end closure, 23 ~
25 DEG C of 5 ~ 7d of culture, must plant bacterium mycelia, will plant bacterium mycelia and are seeded to second class inoculum hair by the inoculum concentration that mass fraction is 5%
Ferment culture medium, cultivation temperature is 23 ~ 25 DEG C, initial pH6.5,60 ~ 100r/min of rotating speed, ventilation quantity 1V/V/min, cultivates 5 ~ 6d,
Button mushroom fermentation culture is obtained, by button mushroom fermentation culture in mass ratio 1:10 are seeded in solid state cultivation kind culture medium
Expand culture, 23 ~ 25 DEG C of 5 ~ 7d of culture, obtain liquid button mushroom and cure culture；
（2）Take vermiculite in mass ratio 1:8 with water Hybrid Heating, obtain vermiculite mixed solution, into vermiculite mixed solution in mass ratio
9:2:3 add odium stearate, tetraisopropyl titanate is reacted as modifying agent, stirring, pull vermiculite out, dry, obtain PERFORMANCE OF MODIFIED VERMICULITE, to
In mass ratio 3 in PERFORMANCE OF MODIFIED VERMICULITE:2 addition polyglutamic acids are uniformly mixed, and be crushed 500 mesh sieves, are obtained water conservation mixed-powder；
（3）Wheat straw is cultivated into decomposed material and piles material heap, heap overlying PVC plastic film, film ventilation is taken off every 24h, is passed through steam heating material
Heap, when material temperature rises to 50 ~ 53 DEG C, carries out turning, repeats turning 3 ~ 4 times, and ferment 13 ~ 15d, obtains fermentation material heap, will ferment
Expect that heap loads polypropylene plastics pocket, be uniformly inoculated with liquid button mushroom curing culture by the inoculum concentration of mass fraction 10%
To fermentation material heap surface, tightening sack makes strain combine closely with the bed of material, is cultivated 25 ~ 30 days at 20 ~ 25 DEG C, obtains button mushroom bacterium
Silk；
（4）The polypropylene plastics pocket for covering with button mushroom mycelia is taken off bag to be placed in plastic crate, is first uniformly spread in hyphal surface
One layer of water conservation powder, thickness about 0.8 ~ 1cm, repaves one layer of peat earthing, thickness about 3 ~ 3.5cm, water spray, protects peat earthing face
Moistening is held, keeps relative air humidity 80% ~ 90%, temperature control is at 15 ~ 20 DEG C, you can turns out agaricus bisporus.
Embodiment 1
Bacterial strain：Agaricus bisporus, is preserved in Hua Zhong Agriculture University's Culture Collection.
Plating medium：According to the mass fraction, 150 parts of potato, 15 parts of sucrose, KH are taken2PO41 part, MgSO4
7H2O0.05 parts, 15 parts of agar, 1000 parts of water, pH natures.
Level liquid bacterium culture medium：According to the mass fraction, 30m30 parts of brewer's wort, 8 parts of corn flour, KH are taken2PO40.8 part,
MgSO40.3 part, VB10.01 part, 1000 parts of water, pH6.5 ± 0.2.
Second class inoculum fermentation medium：According to the mass fraction, 18 parts of wheat bran is taken, 5 parts of corn flour, 1 part of peptone,
KH2PO40.6 part, MgSO40.3 part, VB10.01 part, 0.05 part of fresh food oil, 1000 parts of water, pH6.5 ± 0.2.
Solid pedigree seed culture medium：According to the mass fraction, 900 parts of wheat, 10 parts of excrement grass, 5 parts of lime, 5 parts of gypsum, water are taken
1500 parts, pH6.5 ± 0.2.
Solid state cultivation kind culture medium：According to the mass fraction, 800 parts of wheat is taken, 80 parts of wheat bran, excrement is 7 parts careless, 10 parts of lime,
8 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2.
Wheat straw cultivates decomposed material：According to the mass fraction, 500 parts of wheat straw is taken, 200 parts of oxen and horses excrement, 50 parts of cake fertilizer, 3 parts of urea,
25 parts of gypsum, 10 parts of calcium superphosphate, 8 parts of lime, 1500 parts of water, pH6.5 ± 0.2.
A kind of cultural method of agaricus bisporus, includes the following steps：
（1）Agaricus bisporus mushroom is seeded in plating medium, 23 DEG C of culture 5d must activate White mushroom mycelia, and picking activation is double
Spore mushroom silk is seeded to level liquid bacterium culture medium culture, and 23 DEG C, rotating speed 150r/min shaken cultivation 5d, obtain first class inoculum,
First class inoculum is seeded in solid pedigree seed culture medium and is cultivated, after mycelia is covered with, all original seed mycelium inoculations of picking to solid are planted
In kind culture medium Bag Material, the specification of polypropylene cultigen bag is 12cm × l8cm × 0.005cm, and an end closure, 23 DEG C are cultivated 5d,
Bacterium mycelia must be planted, bacterium mycelia will be planted and be seeded to second class inoculum fermentation medium by the inoculum concentration that mass fraction is 5%, cultivated
Temperature is 23 DEG C, initial pH6.5, rotating speed 60r/min, ventilation quantity 1V/V/min, cultivates 5d, obtains button mushroom fermentation culture,
By button mushroom fermentation culture in mass ratio 1:10 are seeded to expansion culture in solid state cultivation kind culture medium, and 23 DEG C are cultivated 5d,
Obtain liquid button mushroom and cure culture；
（2）Take vermiculite in mass ratio 1:8 with water Hybrid Heating, obtain vermiculite mixed solution, into vermiculite mixed solution in mass ratio
9:2:3 add odium stearate, tetraisopropyl titanate is reacted as modifying agent, stirring, pull vermiculite out, dry, obtain PERFORMANCE OF MODIFIED VERMICULITE, to
In mass ratio 3 in PERFORMANCE OF MODIFIED VERMICULITE:2 addition polyglutamic acids are uniformly mixed, and be crushed 500 mesh sieves, are obtained water conservation mixed-powder；
（3）Wheat straw is cultivated into decomposed material and piles material heap, heap overlying PVC plastic film, film ventilation is taken off every 24h, is passed through steam heating material
Heap, when material temperature rises to 50 DEG C, carries out turning, repeats turning 3 times, and ferment 13d, obtains fermentation material heap, fermentation material heap is loaded
Polypropylene plastics pocket, fermentation material is uniformly seeded to by the inoculum concentration of mass fraction 10% by liquid button mushroom curing culture
Heap surface, tightening sack makes strain combine closely with the bed of material, is cultivated 25 days at 20 DEG C, obtains button mushroom mycelia；
（4）After culture, the material in polypropylene plastics is poured into plastic crate, first the material table in polypropylene plastics
Face uniformly spreads one layer of water conservation powder, and thickness about 0.8cm, repaves one layer of peat earthing, thickness about 3cm, water spray, makes peat earthing face
Moistening is kept, it is 70% peat earthing face is kept humidity, keeps relative air humidity 80%, and temperature control is at 15 DEG C, culture,
Up to agaricus bisporus.
Embodiment 2
Bacterial strain：Agaricus bisporus, is preserved in Hua Zhong Agriculture University's Culture Collection.
Plating medium：According to the mass fraction, 170 parts of potato, 17 parts of sucrose, KH are taken2PO41 part, MgSO4
7H2O0.07 parts, 17 parts of agar, 1000 parts of water, pH natures.
Level liquid bacterium culture medium：According to the mass fraction, 30m33 parts of brewer's wort, 9 parts of corn flour, KH are taken2PO41.0 parts,
MgSO40.4 part, VB10.01 part, 1000 parts of water, pH6.5 ± 0.2.
Second class inoculum fermentation medium：According to the mass fraction, 19 parts of wheat bran is taken, 7 parts of corn flour, 1 part of peptone,
KH2PO40.7 part, MgSO40.4 part, VB10.01 part, 0.06 part of fresh food oil, 1000 parts of water, pH6.5 ± 0.2.
Solid pedigree seed culture medium：According to the mass fraction, 950 parts of wheat, 13 parts of excrement grass, 6 parts of lime, 7 parts of gypsum, water are taken
1500 parts, pH6.5 ± 0.2.
Solid state cultivation kind culture medium：According to the mass fraction, 850 parts of wheat is taken, 85 parts of wheat bran, excrement is 8 parts careless, 11 parts of lime,
9 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2.
Wheat straw cultivates decomposed material：According to the mass fraction, 550 parts of wheat straw is taken, 225 parts of oxen and horses excrement, 60 parts of cake fertilizer, 4 parts of urea,
27 parts of gypsum, 13 parts of calcium superphosphate, 9 parts of lime, 1500 parts of water, pH6.5 ± 0.2.
A kind of cultural method of agaricus bisporus, includes the following steps：
（1）Agaricus bisporus mushroom is seeded in plating medium, 24 DEG C of culture 6d must activate White mushroom mycelia, and picking activation is double
Spore mushroom silk is seeded to level liquid bacterium culture medium culture, and 24 DEG C, rotating speed 150r/min shaken cultivation 5d, obtain first class inoculum,
First class inoculum is seeded in solid pedigree seed culture medium and is cultivated, after mycelia is covered with, all original seed mycelium inoculations of picking to solid are planted
In kind culture medium Bag Material, the specification of polypropylene cultigen bag is 12cm × l8cm × 0.005cm, and an end closure, 23 ~ 25 DEG C are cultivated
6d, must plant bacterium mycelia, will plant bacterium mycelia and be seeded to second class inoculum fermentation medium by the inoculum concentration that mass fraction is 5%, train
Foster temperature is 24 DEG C, initial pH6.5, rotating speed 80r/min, ventilation quantity 1V/V/min, cultivates 5d, obtains button mushroom fermented and cultured
Liquid, by button mushroom fermentation culture in mass ratio 1:10 are seeded to expansion culture in solid state cultivation kind culture medium, 24 DEG C of cultures
6d, obtains liquid button mushroom and cures culture；
（2）Take vermiculite in mass ratio 1:8 with water Hybrid Heating, obtain vermiculite mixed solution, into vermiculite mixed solution in mass ratio
9:2:3 add odium stearate, tetraisopropyl titanate is reacted as modifying agent, stirring, pull vermiculite out, dry, obtain PERFORMANCE OF MODIFIED VERMICULITE, to
In mass ratio 3 in PERFORMANCE OF MODIFIED VERMICULITE:2 addition polyglutamic acids are uniformly mixed, and be crushed 500 mesh sieves, are obtained water conservation mixed-powder；
（3）Wheat straw is cultivated into decomposed material and piles material heap, heap overlying PVC plastic film, film ventilation is taken off every 24h, is passed through steam heating material
Heap, when material temperature rises to 52 DEG C, carries out turning, repeats turning 3 times, and ferment 14d, obtains fermentation material heap, fermentation material heap is loaded
Polypropylene plastics pocket, fermentation material is uniformly seeded to by the inoculum concentration of mass fraction 10% by liquid button mushroom curing culture
Heap surface, tightening sack makes strain combine closely with the bed of material, is cultivated 27 days at 24 DEG C, obtains button mushroom mycelia；
（4）After culture, the material in polypropylene plastics is poured into plastic crate, first the material table in polypropylene plastics
Face uniformly spreads one layer of water conservation powder, and thickness about 0.9cm, repaves one layer of peat earthing, thickness about 3.2cm, water spray, makes peat earthing
Face keeps moistening, and it is 70% peat earthing face is kept humidity, keeps relative air humidity 85%, temperature control is at 17 DEG C, training
Support, up to agaricus bisporus.
Embodiment 3
Bacterial strain：Agaricus bisporus, is preserved in Hua Zhong Agriculture University's Culture Collection.
Plating medium：According to the mass fraction, 200 parts of potato, 20 parts of sucrose, KH are taken2PO42 parts, MgSO4
7H2O0.1 parts, 20 parts of agar, 1000 parts of water, pH natures.
Level liquid bacterium culture medium：According to the mass fraction, 30m35 parts of brewer's wort, 10 parts of corn flour, KH are taken2PO41.2
Part, MgSO40.5 part, VB10.02 part, 1000 parts of water, pH6.5 ± 0.2.
Second class inoculum fermentation medium：According to the mass fraction, 20 parts of wheat bran is taken, 10 parts of corn flour, 3 parts of peptone,
KH2PO40.8 part, MgSO40.5 part, VB10.02 part, 0.08 part of fresh food oil, 1000 parts of water, pH6.5 ± 0.2.
Solid pedigree seed culture medium：According to the mass fraction, 1000 parts of wheat, 15 parts of excrement grass, 8 parts of lime, 10 parts of gypsum, water are taken
1500 parts, pH6.5 ± 0.2.
Solid state cultivation kind culture medium：According to the mass fraction, 900 parts of wheat is taken, 90 parts of wheat bran, excrement is 10 parts careless, 12 parts of lime,
10 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2.
Wheat straw cultivates decomposed material：According to the mass fraction, 600 parts of wheat straw is taken, 250 parts of oxen and horses excrement, 70 parts of cake fertilizer, 5 parts of urea,
30 parts of gypsum, 15 parts of calcium superphosphate, 10 parts of lime, 1500 parts of water, pH6.5 ± 0.2.
A kind of cultural method of agaricus bisporus, includes the following steps：
（1）Agaricus bisporus mushroom is seeded in plating medium, 25 DEG C of culture 7d must activate White mushroom mycelia, and picking activation is double
Spore mushroom silk is seeded to level liquid bacterium culture medium culture, and 25 DEG C, rotating speed 150r/min shaken cultivation 6d, obtain first class inoculum,
First class inoculum is seeded in solid pedigree seed culture medium and is cultivated, after mycelia is covered with, all original seed mycelium inoculations of picking to solid are planted
In kind culture medium Bag Material, the specification of polypropylene cultigen bag is 12cm × l8cm × 0.005cm, and an end closure, 25 DEG C are cultivated 7d,
Bacterium mycelia must be planted, bacterium mycelia will be planted and be seeded to second class inoculum fermentation medium by the inoculum concentration that mass fraction is 5%, cultivated
Temperature is 25 DEG C, initial pH6.5, rotating speed 100r/min, ventilation quantity 1V/V/min, cultivates 6d, obtains button mushroom fermentation culture,
By button mushroom fermentation culture in mass ratio 1:10 are seeded to expansion culture in solid state cultivation kind culture medium, and 25 DEG C are cultivated 7d,
Obtain liquid button mushroom and cure culture；
（2）Take vermiculite in mass ratio 1:8 with water Hybrid Heating, obtain vermiculite mixed solution, into vermiculite mixed solution in mass ratio
9:2:3 add odium stearate, tetraisopropyl titanate is reacted as modifying agent, stirring, pull vermiculite out, dry, obtain PERFORMANCE OF MODIFIED VERMICULITE, to
In mass ratio 3 in PERFORMANCE OF MODIFIED VERMICULITE:2 addition polyglutamic acids are uniformly mixed, and be crushed 500 mesh sieves, are obtained water conservation mixed-powder；
（3）Wheat straw is cultivated into decomposed material and piles material heap, heap overlying PVC plastic film, film ventilation is taken off every 24h, is passed through steam heating material
Heap, when material temperature rises to 53 DEG C, carries out turning, repeats turning 4 times, and ferment 15d, obtains fermentation material heap, fermentation material heap is loaded
Polypropylene plastics pocket, fermentation material is uniformly seeded to by the inoculum concentration of mass fraction 10% by liquid button mushroom curing culture
Heap surface, tightening sack makes strain combine closely with the bed of material, is cultivated 30 days at 25 DEG C, obtains button mushroom mycelia；
（4）After culture, the material in polypropylene plastics is poured into plastic crate, first the material table in polypropylene plastics
Face uniformly spreads one layer of water conservation powder, and thickness about 1cm, repaves one layer of peat earthing, thickness about 3.5cm, water spray, makes peat earthing face
Moistening is kept, it is 70% peat earthing face is kept humidity, keeps relative air humidity 90%, and temperature control is at 20 DEG C, culture,
Up to agaricus bisporus.
Comparative example：The agaricus bisporus of Xi'an bio tech ltd production
Method：The agaricus bisporus prepared by the embodiment and comparative example of equivalent is taken, the agaricus bisporus mycelia of equivalent is placed in one,
According to GB12530 detection mycelial growth rates and hyphal diameter.
The specific detection case of agaricus bisporus such as table 1
Table 1
From the foregoing, it will be observed that the agaricus bisporus culture medium prepared by the present invention can be effectively facilitated the fast-growth of agaricus bisporus mycelia,
And obtained mycelium growth vigor is good, diameter is big, and yield is high, is a kind of safety and efficient agaricus bisporus culture medium, is worthy to be popularized
And use.
Claims (7)
1. a kind of cultural method of agaricus bisporus, it is characterised in that the preparation method includes the following steps：
（1）Agaricus bisporus mushroom is seeded in plating medium, is cultivated, White mushroom mycelia, picking activation button mushroom must be activated
Silk is seeded to level liquid bacterium culture medium culture, obtains first class inoculum, first class inoculum is seeded in solid pedigree seed culture medium and is trained
Support, after mycelia is covered with, all original seed mycelium inoculations of picking to solid are planted in culture medium Bag Material, an end closure, and culture, must plant
Bacterium mycelia, by plantation bacterium mycelium inoculation to second class inoculum fermentation medium culture, obtains button mushroom fermentation culture, by White mushroom
Bacterium fermentation culture, which is seeded in solid state cultivation kind culture medium, expands culture, obtains liquid button mushroom and cures culture；
（2）By vermiculite and water Hybrid Heating, vermiculite mixed solution is obtained, odium stearate, metatitanic acid four are added into vermiculite mixed solution
Isopropyl ester, stirring reaction, pulls vermiculite out, dry, obtains PERFORMANCE OF MODIFIED VERMICULITE, and polyglutamic acid is added into PERFORMANCE OF MODIFIED VERMICULITE and is uniformly mixed, powder
Broken sieving, obtains water conservation mixed-powder；
（3）Wheat straw is cultivated into decomposed material and piles material heap, heap overlying PVC plastic film, film ventilation is taken off every 24h, is passed through steam heating material
Heap, when material temperature rises to 50 ~ 53 DEG C, carries out turning, repeats turning 3 ~ 4 times, fermentation, obtains fermentation material heap, fermentation material is stowed
Enter polypropylene plastics pocket, liquid button mushroom curing culture is uniformly seeded to fermentation material heap surface, is cultivated；
（4）After culture, the material in polypropylene plastics is poured into plastic crate, first the material table in polypropylene plastics
Face uniformly spreads one layer of water conservation powder, repaves one layer of peat earthing, sprays water, and it is 70% peat earthing face is kept humidity, keeps sky
Gas relative humidity 80% ~ 90%, temperature control is at 15 ~ 20 DEG C, culture, up to agaricus bisporus.
2. the cultural method of Dual Mushroom mushroom according to claim 1, it is characterised in that the step（1）Middle plate culture
Base：According to the mass fraction, 150 ~ 200 parts of potato, 15 ~ 20 parts of sucrose, KH are taken2PO41 ~ 2 part, MgSO47H2O0.05~0.1
Part, 15 ~ 20 parts of agar, 1000 parts of water, pH natures；Level liquid bacterium culture medium：According to the mass fraction, take brewer's wort 30m30 ~
35 parts, 8 ~ 10 parts of corn flour, KH2PO40.8 ~ 1.2 part, MgSO40.3 ~ 0.5 part, VB10.01 ~ 0.02 part, 1000 parts of water, pH6.5
±0.2；
Second class inoculum fermentation medium：According to the mass fraction, 18 ~ 20 parts of wheat bran is taken, 5 ~ 10 parts of corn flour, 1 ~ 3 part of peptone,
KH2PO40.6 ~ 0.8 part, MgSO40.3 ~ 0.5 part, VB10.01 ~ 0.02 part, 0.05 ~ 0.08 part of fresh food oil, 1000 parts of water,
pH6.5±0.2；Solid pedigree seed culture medium：According to the mass fraction, 900 ~ 1000 parts of wheat, 10 ~ 15 parts of excrement grass, lime 5 ~ 8 are taken
Part, 5 ~ 10 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2；Solid state cultivation kind culture medium：According to the mass fraction, wheat 800 ~ 900 is taken
Part, 80 ~ 90 parts of wheat bran, 7 ~ 10 parts of excrement grass, 10 ~ 12 parts of lime, 8 ~ 10 parts of gypsum, 1500 parts of water, pH6.5 ± 0.2.
3. the cultural method of Dual Mushroom mushroom according to claim 1, it is characterised in that the step（1）Middle first class inoculum
Condition of culture is：23 ~ 25 DEG C, 5 ~ 6d of rotating speed 150r/min shaken cultivations；The specification of polypropylene cultigen bag is 12cm × l8cm
×0.005cm；The inoculum concentration for planting bacterium mycelia is that mass fraction is 5%；The condition of culture of button mushroom fermentation culture is：Training
Foster temperature is 23 ~ 25 DEG C, initial pH6.5,60 ~ 100r/min of rotating speed, ventilation quantity 1V/V/min, cultivates 5 ~ 6d；Button mushroom is sent out
Ferment nutrient solution in mass ratio 1:10 are seeded to solid state cultivation kind culture；Liquid button mushroom cure culture condition of culture for 23 ~
25 DEG C of 5 ~ 7d of culture.
4. the cultural method of Dual Mushroom mushroom according to claim 1, it is characterised in that the step（2）Middle vermiculite is with going
The mass ratio of ionized water is 1:8；With the mass ratio of odium stearate, tetraisopropyl titanate it is 9 in vermiculite mixed solution:2:3；It is modified
The mass ratio of vermiculite and polyglutamic acid is 3:2.
5. the cultural method of Dual Mushroom mushroom according to claim 1, it is characterised in that the step（3）Middle wheat straw cultivation
Decomposed material：According to the mass fraction, 500 ~ 600 parts of wheat straw, 200 ~ 250 parts of oxen and horses excrement, 50 ~ 70 parts of cake fertilizer, 3 ~ 5 parts of urea, stone are taken
25 ~ 30 parts of cream, 10 ~ 15 parts of calcium superphosphate, 8 ~ 10 parts of lime, 1500 parts of water, pH6.5 ± 0.2.
6. the cultural method of Dual Mushroom mushroom according to claim 1, it is characterised in that the step（3）Middle fermentation condition
For 50 ~ 53 DEG C, 13 ~ 15d of fermentation；Liquid button mushroom curing culture is uniformly inoculated with by the inoculum concentration of mass fraction 10%
To fermentation material heap surface, condition of culture is to be cultivated 25 ~ 30 days at 20 ~ 25 DEG C.
7. the cultural method of Dual Mushroom mushroom according to claim 1, it is characterised in that the step（4）Middle water conservation powder
Layer thickness about 0.8 ~ 1cm, peat earthing layer thickness about 3 ~ 3.5cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711114617.6A CN107896818A (en) | 2017-11-13 | 2017-11-13 | A kind of cultural method of agaricus bisporus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711114617.6A CN107896818A (en) | 2017-11-13 | 2017-11-13 | A kind of cultural method of agaricus bisporus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107896818A true CN107896818A (en) | 2018-04-13 |
Family
ID=61845017
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711114617.6A Pending CN107896818A (en) | 2017-11-13 | 2017-11-13 | A kind of cultural method of agaricus bisporus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107896818A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109076883A (en) * | 2018-08-28 | 2018-12-25 | 重庆市药物种植研究所 | A kind of liquid spawn-solid state cultivation kind cultural method of the rotten edible mushroom of grass |
| CN109566261A (en) * | 2018-12-10 | 2019-04-05 | 南京财经大学 | A method of promoting agaricus bisporus growth and preservation quality |
| CN112042470A (en) * | 2020-09-15 | 2020-12-08 | 贵州土老磨农业发展有限公司 | Leavening agent suitable for edible fungi and method for fermenting edible fungi by utilizing leavening agent |
| CN112703966A (en) * | 2020-12-25 | 2021-04-27 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for efficiently cultivating oospore oudemansiella mucida by using water-retaining material |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284750A (en) * | 2008-05-23 | 2008-10-15 | 贾恩茂 | Culture medium for cultivating agaricus bisporus and cultivating process thereof |
| CN103155784A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Cultural method for brown mushroom cultispecies |
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN103843585A (en) * | 2014-03-17 | 2014-06-11 | 周桃英 | High-yield agaricus bisporus cultivation method |
| CN105567572A (en) * | 2016-01-04 | 2016-05-11 | 山东省科创食用菌产业技术研究院 | Preparation technology of agaricus bisporus strain |
| CN106673811A (en) * | 2016-12-11 | 2017-05-17 | 唐林元 | Preparation method of soil-loosening gas-permeable edible mushrooms water-retaining agent |
-
2017
- 2017-11-13 CN CN201711114617.6A patent/CN107896818A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284750A (en) * | 2008-05-23 | 2008-10-15 | 贾恩茂 | Culture medium for cultivating agaricus bisporus and cultivating process thereof |
| CN103155784A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Cultural method for brown mushroom cultispecies |
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN103843585A (en) * | 2014-03-17 | 2014-06-11 | 周桃英 | High-yield agaricus bisporus cultivation method |
| CN105567572A (en) * | 2016-01-04 | 2016-05-11 | 山东省科创食用菌产业技术研究院 | Preparation technology of agaricus bisporus strain |
| CN106673811A (en) * | 2016-12-11 | 2017-05-17 | 唐林元 | Preparation method of soil-loosening gas-permeable edible mushrooms water-retaining agent |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109076883A (en) * | 2018-08-28 | 2018-12-25 | 重庆市药物种植研究所 | A kind of liquid spawn-solid state cultivation kind cultural method of the rotten edible mushroom of grass |
| CN109566261A (en) * | 2018-12-10 | 2019-04-05 | 南京财经大学 | A method of promoting agaricus bisporus growth and preservation quality |
| CN112042470A (en) * | 2020-09-15 | 2020-12-08 | 贵州土老磨农业发展有限公司 | Leavening agent suitable for edible fungi and method for fermenting edible fungi by utilizing leavening agent |
| CN112703966A (en) * | 2020-12-25 | 2021-04-27 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for efficiently cultivating oospore oudemansiella mucida by using water-retaining material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103190330B (en) | Soilless biogas slurry cultivation method of water spinach | |
| CN101697695B (en) | Method for cultivating edible straw rotting fungus by using water hyacinth as nutrient medium | |
| CN102138441B (en) | Cultivation material composition for producing high-quality agaricus blazei and manure-free rice straw raw material cultivation method | |
| CN101085981B (en) | Edible mushroom liquid strain solidifying processing method | |
| CN104206286B (en) | Scaled cattle farms cow dung cascaded utilization of energy combines method of disposal | |
| CN106234034A (en) | A kind of Agaricus Bisporus industrialization breeding method | |
| CN107896818A (en) | A kind of cultural method of agaricus bisporus | |
| CN103503696B (en) | Method for culturing straw mushrooms with integrate corncob raw materials as substrate | |
| CN103172421A (en) | Treatment method of fruit/vegetable waste | |
| CN107306671A (en) | Pleurotus eryngii waste material prepares the compost and cultural method of White mushroom | |
| CN104641942A (en) | Method for cultivating oyster mushroom on mulberry twigs | |
| CN112369276A (en) | Culture medium for cultivating stropharia rugoso-annulata by using pleurotus eryngii dregs as well as preparation method and application of culture medium | |
| CN105724055B (en) | A method of improving agaricus bisporus yield using needle mushroom dreg | |
| CN106673760A (en) | Method for industrializedly cultivating agaricus bisporus by utilizing wood rotting fungus dreg | |
| CN108029503A (en) | The preparation method of virus-free potato primary stock special bio organic substrate | |
| CN109392605B (en) | Method for cultivating agaricus bisporus by using eucalyptus bark | |
| CN107926481B (en) | Pure straw cultivation method for straw mushrooms | |
| CN208821351U (en) | A kind of dictyophora culture medium | |
| CN108689743A (en) | Batch production agaricus bisporus production technology | |
| Savoie et al. | Biomethane digestate from horse manure, a new waste usable in compost for growing the button mushroom, Agaricus bisporus | |
| CN104496601A (en) | Method for preparing organic humus soil | |
| CN109169167A (en) | One kind manufacturing forestry seedling medium and preparation method thereof by raw material of mushroom bran | |
| CN114868597B (en) | Planting method for interplanting edible mushrooms in tea garden | |
| CN101531545A (en) | Method for producing pleurotus ostreatus by utilizing biogas residue | |
| CN104725115A (en) | Preparation method of flammulina velutipes cultivation material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180413 |
|
| RJ01 | Rejection of invention patent application after publication |