CN108456646A - A kind of Boydii Phellinus pore fungi and its application - Google Patents
A kind of Boydii Phellinus pore fungi and its application Download PDFInfo
- Publication number
- CN108456646A CN108456646A CN201810491265.4A CN201810491265A CN108456646A CN 108456646 A CN108456646 A CN 108456646A CN 201810491265 A CN201810491265 A CN 201810491265A CN 108456646 A CN108456646 A CN 108456646A
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- Prior art keywords
- phellinus
- boydii
- thousand
- culture medium
- pore fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of Boydii Phellinus pore fungi and its applications; the bacterial strain is on November 23rd, 2017 in the preservation of China General Microbiological center; preserving number is CGMCC No.14799; Boydii Phellinus pore fungi (Sanghuangporus baumii) using the present invention plants Phellinus; not only it is higher by mushroom rate; and Phellinus sporophore growth is good; disease and insect resistance is high; cultivation technique is simple and practicable; management is relatively easy; it is the improved seeds of quantization cultivation Phellinus fructification, fully meets the needs of market.
Description
Technical field
The invention belongs to biotechnologies, specifically, being related to a kind of Boydii Phellinus pore fungi and its application.
Background technology
Phellinus is a kind of edible and medicinal fungi, and it is usage history to have long in China, and modern study confirms Phellinus granulose energy
It enough relieves pain, lose the appetite, the distinctive symptom of cancers, the exploitation of Phellinus such as weight loss and tired burnout can not only be enriched
Medicinal material market and capture great difficult and complicated illness, moreover it is possible to promote local industries development and surplus rural labor force to obtain employment poverty alleviation, have
Great meaning and considerable foreground.But Phellinus bacterium yield is extremely limited, and wild Phellinus is endangered, currently, Phellinus is artificial
The sawdust that planting type is mostly crushed with broad leaf trees branch such as mulberry tree, willow, toothed oak trees is main culturing raw material, with greenhouse plastic bag cultivation ground
Go out based on Huang, the artificial cultivation difficulty of Phellinus is big, and cultivating rate is small, and survival rate is low.
In view of this special to propose the present invention.
Invention content
The technical problem to be solved in the present invention be to overcome the deficiencies of the prior art and provide a kind of Boydii Phellinus pore fungi and its
Using being not only higher by mushroom rate, but also Phellinus sporophore growth is good, disease and insect resistance is high.
In order to solve the above technical problems, the present invention is using the basic conception of technical solution：
A kind of thousand side of Ji of Boydii Phellinus pore fungi 1, Classification And Nomenclature be Sanghuangporus baumii, the bacterial strain in
On November 23rd, 2017, preserving number was CGMCC No.14799 in the preservation of China General Microbiological center.
Application of thousand side of Ji of Boydii Phellinus pore fungi 1 in Phellinus plantation, includes the following steps：Cultivate claim 1 institute
Thousand side of Ji of Phellinus bacterial strain stated 1 obtains Phellinus with plantation.
Further, the specific steps are：
Step S1, prepares culture medium, after weighing raw material in culture medium prescription ratio, is fitted into edible mushroom bag special or bottle,
Sealed bag or bottleneck sterilize, and slow cooling is to room temperature after sterilizing；
Step S2 is inoculated with bacterium germination, thousand side of Ji of Phellinus bacterial strain 1 is aseptically accessed after culture medium cooling, in 22
Culture, can cover with, obtain the culture medium of thousand Ji side 1 for 30-45 days in DEG C-28 DEG C of thermostatic chamber；
Step S3, section wood bacterium germination, takes broad leaf tree juggle, and juggle is packed into polypropylene plastics pocket, tightens sack and sterilizes, is cold
But, later aseptically, the culture medium of thousand Ji side 1 is added in polypropylene plastics pocket, tightens sack, cultivated 30-50 days
Later, segment wood cultivated bag is cut into thick 0.5-1 centimetre of thin mouth with the knife after disinfection；
Step S4 buries native fruiting, puts soil in order, and section wood is vertically buried in the earth, and inlet part accounts for 2/3-3/4, development
Temperature is 22 DEG C -28 DEG C, air humidity 90%, native stage casing wood water content 150% -180%, scattering illumination transmissivity 10%
Left and right, buds into Phellinus fructification after 1-2 months.
Further, in the step S1, the raw material group of culture medium becomes：Sawdust 78%, wheat bran 15%, analysis for soybean powder or jade
The moisture of rice flour 5%, lime 1%, gypsum 1%, culture medium is 65%.
Further, in the step S1, the temperature of sterilizing is 100 DEG C -105 DEG C, and the time of sterilizing is 8-12 hours.
Further, in the step S3, the condition of sterilizing is：High pressure steam sterilization maintains under 0.15MPa pressure
1.5-2 hours, or maintained 10-22 hours at 100 DEG C-105 DEG C.
After adopting the above technical scheme, the present invention has the advantages that compared with prior art.
The present invention provides a kind of Boydii Phellinus pore fungi, and the bacterial strain is on November 23rd, 2017 at China General Microbiological center
Preservation, preserving number are CGMCC No.14799, Boydii Phellinus pore fungi (Sanghuangporus baumii) kind using the present invention
Phellinus is planted, is not only higher by mushroom rate, but also Phellinus sporophore growth is good, disease and insect resistance is high, and cultivation technique is simple and practicable, management
Relatively easily, it is the improved seeds of quantization cultivation Phellinus fructification, fully meets the needs of market.
Specific implementation mode
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below to the technical side in embodiment
Case is clearly and completely described, and the following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..If not
It refers in particular to, all parts, percentage are unit of weight.
Embodiment 1
A kind of thousand side of Ji of Boydii Phellinus pore fungi 1, Classification And Nomenclature be Sanghuangporus baumii, the bacterial strain in
On November 23rd, 2017, preserving number was CGMCC No.14799 in the preservation of China General Microbiological center.
The strain culturing：(1) culture medium is prepared：Sawdust 78%, wheat bran 15%, analysis for soybean powder (corn flour) 5%, lime 1%,
Gypsum 1%, water content 65%, pH are natural；(2) (bottle) is packed：Thousand strain growths of Ji side 1 are aerobic strong, and bag (bottle) mouth is slightly larger,
It will prepare and the compost mixed well of water is added to be packed into, and sack is tightened and (covered tightly) be maintained 8-12 hours at 100 DEG C -105 DEG C, go out
Cooling rate needs slow at the end of bacterium, prevents bag (bottle) from rupturing；(3) it is inoculated with bacterium germination：Thousand are aseptically accessed after cooling
The bacterial strain of Ji side 1 can cover with for 30-45 days in culture in 22 DEG C-28 DEG C of thermostatic chambers.
Embodiment 2
Application of thousand side of Ji of Boydii Phellinus pore fungi 1 in Phellinus plantation, using the Boydii Phellinus hole in above-described embodiment 1
Bacterium (Sanghuangporus baumii) is used as strain, specific steps：
Step S1, prepares culture medium, weighs raw material in culture medium prescription ratio, Cultivar culture medium includes following weight ratio
Raw material：Sawdust 78%, wheat bran 15%, analysis for soybean powder (corn flour) 5%, lime 1%, gypsum 1%, water content 65%, pH are natural；
Thousand strain growths of Ji side 1 are aerobic strong, and bag (bottle) mouth is slightly larger, the compost for preparing and adding water to mix well are packed into, by sack
It tightens and (covers tightly) and maintained 8-12 hours at 100 DEG C -105 DEG C, cooling rate needs slow at the end of sterilizing, prevents bag (bottle) broken
It splits.
Step S2 is inoculated with bacterium germination, thousand side of Ji of Phellinus bacterial strain 1 is aseptically accessed after culture medium cooling, in 22
Culture, can cover with, obtain the culture medium of thousand Ji side 1 for 30-45 days in DEG C-28 DEG C of thermostatic chamber.
Step S3, section wood bacterium germination, the trees under taking broad leaf tree (mulberry tree, willow, birch, toothed oak tree, robur) to fell and branch
It is cut into 15-45 centimetres of juggle, juggle is packed into polypropylene plastics pocket, tightens sack；Then high pressure steam sterilization,
It maintains 1.5-2 hours under 0.15MPa pressure, or is maintained 10-22 hours at 100 DEG C-105 DEG C, after sterilizing, taking-up is placed on
Clean room cooling is aseptically packed into the culture medium of thousand Ji side 1 in sack, tightens sack, cultural hypha 30-
After 50 days, segment wood cultivated bag is cut into thick 0.5-1 centimetre of thin mouth with the knife after disinfection.
Step S4 buries native fruiting, buries the good soil of native prefinishing, section wood is separated, and is spaced 8-10 centimetres, is then hung down
It is directly buried in the earth, inlet part accounts for 2/3-3/4, and thousand developmental temperatures of Ji side 1 are 22 DEG C-28 DEG C, air humidity 90%, soil
Stage casing wood water content 150%-180%, scattering illumination transmissivity 10% or so, went out in 1-2 months or so the bacterial strains of thousand Ji side 1
Mushroom buds into Phellinus fructification.
The above is only presently preferred embodiments of the present invention, is not intended to limit the present invention in any form, though
So the present invention has been disclosed as a preferred embodiment, and however, it is not intended to limit the invention, any technology people for being familiar with this patent
Member without departing from the scope of the present invention, when the technology contents using above-mentioned prompt make it is a little change or be modified to
The equivalent embodiment of equivalent variations, it is right according to the technical essence of the invention as long as being the content without departing from technical solution of the present invention
Any simple modification, equivalent change and modification made by above example, in the range of still falling within the present invention program.
Claims (6)
1. a kind of thousand side of Ji of Boydii Phellinus pore fungi 1, which is characterized in that its Classification And Nomenclature is Sanghuangporus baumii,
For the bacterial strain on November 23rd, 2017 in the preservation of China General Microbiological center, preserving number is CGMCC No.14799.
2. application of thousand side of Ji of Boydii Phellinus pore fungi 1 as described in claim 1 in Phellinus plantation, which is characterized in that including
Following steps：It cultivates thousand side of Ji of Boydii Phellinus pore fungi 1 described in claim 1 and Phellinus is obtained with plantation.
3. application of thousand side of Ji of Boydii Phellinus pore fungi 1 according to claim 2 in Phellinus plantation, which is characterized in that
The specific steps are：
Step S1, prepares culture medium, after weighing raw material in culture medium prescription ratio, is fitted into edible mushroom bag special or bottle, closing
Bag or bottleneck sterilize, and slow cooling is to room temperature after sterilizing；
Step S2 is inoculated with bacterium germination, thousand side of Ji of Phellinus bacterial strain 1 is aseptically accessed after culture medium cooling, in 22 DEG C -28
DEG C thermostatic chamber in culture, can cover within 30-45 days, obtain the culture medium of thousand Ji side 1；
Step S3, section wood bacterium germination, takes broad leaf tree juggle, and juggle is packed into polypropylene plastics pocket, tightens sack sterilizing, cooling, it
Afterwards aseptically, the culture medium of thousand Ji side 1 is added in polypropylene plastics pocket, tightens sack, after cultivating 30-50 days,
Segment wood cultivated bag is cut into thick 0.5-1 centimetre of thin mouth with the knife after disinfection；
Step S4 buries native fruiting, puts soil in order, section wood is vertically buried in the earth, inlet part accounts for 2/3-3/4, developmental temperature
It is 22 DEG C -28 DEG C, air humidity 90%, native stage casing wood water content 150% -180%, 10% left side of scattering illumination transmissivity
The right side buds into Phellinus fructification after 1-2 months.
4. application of thousand side of Ji of Boydii Phellinus pore fungi 1 according to claim 3 in Phellinus plantation, which is characterized in that
In the step S1, the raw material group of culture medium becomes：Sawdust 78%, wheat bran 15%, analysis for soybean powder or corn flour 5%, lime 1%,
The moisture of gypsum 1%, culture medium is 65%.
5. application of thousand side of Ji of Boydii Phellinus pore fungi 1 according to claim 3 in Phellinus plantation, which is characterized in that
In the step S1, the temperature of sterilizing is 100 DEG C -105 DEG C, and the time of sterilizing is 8-12 hours.
6. application of thousand side of Ji of Boydii Phellinus pore fungi 1 according to claim 3 in Phellinus plantation, which is characterized in that
In the step S3, the condition of sterilizing is：High pressure steam sterilization maintains 1.5-2 hours under 0.15MPa pressure, or 100
DEG C -105 DEG C maintain 10-22 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810491265.4A CN108456646A (en) | 2018-05-21 | 2018-05-21 | A kind of Boydii Phellinus pore fungi and its application |
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| CN201810491265.4A CN108456646A (en) | 2018-05-21 | 2018-05-21 | A kind of Boydii Phellinus pore fungi and its application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109294923A (en) * | 2018-09-07 | 2019-02-01 | 山东省科学院生物研究所 | A kind of biosensor based on immobilization Phellinus thallus |
| CN116240114A (en) * | 2023-01-31 | 2023-06-09 | 山东蓬勃生物科技有限公司 | Phellinus linteus YX2, extract and application thereof |
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Cited By (4)
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| CN116240114B (en) * | 2023-01-31 | 2023-11-03 | 山东蓬勃生物科技有限公司 | Phellinus linteus YX2, extract and application thereof |
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Application publication date: 20180828 |