CN108753624A - A method of preparing degradable package material using Schizophyllum commune for Mycelium - Google Patents
A method of preparing degradable package material using Schizophyllum commune for Mycelium Download PDFInfo
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- CN108753624A CN108753624A CN201810351459.4A CN201810351459A CN108753624A CN 108753624 A CN108753624 A CN 108753624A CN 201810351459 A CN201810351459 A CN 201810351459A CN 108753624 A CN108753624 A CN 108753624A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/466—Bio- or photodegradable packaging materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D2565/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D2565/38—Packaging materials of special type or form
- B65D2565/381—Details of packaging materials of special type or form
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Abstract
The present invention relates to a kind of methods preparing degradable package material using Schizophyllum commune for Mycelium, including step：S101：Compost prepares：By maize straw, wheat stalk, apple sawdust, wheat shell, cotton seed hulls, lime, gypsum is uniformly mixed；Water mixing, sterilizing are added into compost；S102：Strain preculture：Schizophyllum commune liquid spawn is inoculated in the bacterium bag being cooled to room temperature, lid culture is covered；S103：Mold filling culture：In the packaged die that aseptically material for covering with Schizophyllum commune for Mycelium filling has been sterilized, continue to cultivate after being pressed and sealed；S104：Sample drying：After Schizophyllum commune for Mycelium is tied again in the material in mold, sample is taken out out of mold and is dried to obtain product.For the present invention using crop material as primary raw material, apple sawdust, wheat shell is auxiliary material, using Schizophyllum commune for Mycelium as the environment-friendly package material of binder, you can solves the problems, such as existing packaging non-degradable, can also preferably handle crop material and fruit tree.
Description
Technical field
The invention belongs to field of packaging material more particularly to a kind of utilization Schizophyllum commune for Mycelium to prepare degradable package
The method of material.
Background technology
With white pollution increasingly aggravate and natural resources it is increasingly in short supply, degradable, green, environment-friendly package material is got over
Paid attention to by consumers in general to get over.Currently, the correlative study of biomass full degradable product has become the heat studied both at home and abroad
Point.
China is large agricultural country, and corn actual production in 2016 is 2.2463 hundred million tons.It is changed according to corn yield and stalk amount
Calculation relationship：Stalk amount=1.2 × corn yield.China's stalk in 2016 is about 2.6955 hundred million tons.China's maize straw is handled at present
Biogas is mainly made, domestic animal foodstuff is done, makes plant ash returning to the field etc..But using small scale and also a large amount of stalks are incinerated,
Pollution is generated, the wasting of resources is also resulted in.The annual output of China's wheat stalk is about 1.8 hundred million tons or so, is gone out in some areas
The phenomenon that now huddling, disorderly abandon, or even burning, wheat stalk is treated as problem.In fact, wheat stalk is not waste,
But a kind of renewable resource of preciousness.
Apple is the big kind of yield first in all kinds of fruit in China, and China is maximum apple production state in the world.
In these years, China's apple production stablize increase, the whole city's cultivated area of the apple of Yantai in 2015 up to 2,720,000 mu, fruit-bearing forest branch
Pruning rate also adds whole year by year, and discarded fruit-bearing forest branch should what course to follow as a problem.
There are many macro fungis in nature, using in extensive range.Many has been exploited for industrial fermentation and agriculture
Industry produces.Schizophyllum commune（Schizophyllum commune Fr. ）It is a kind of edible medicinal macro fungi, is widely distributed in all over the world,
It is widely used in food industry, medical and health, biochemistry etc..Schizophyllum commune includes mycelium and fructification two parts group
At fructification edible value is high, and matter is tender delicious, has special strong fragrance, mild-natured, has strengthening by means of tonics, sedation, is
High-grade edible and medical fungi.The bacterium mycelium contains more strongly active cellulase, and can generate malic acid, and when mycelia submerged fermentation can
Generate a large amount of organic acids, can also generate regulator heteroauxin, using its mycelium make packaging material there is not yet.
Patent of invention at present（CN106633990A）Using maize straw as the fungi based biomass packaging material of major ingredient and its
Preparation method, patent of invention（CN106633989A）Using bagasse as the fungi based biomass packaging material of major ingredient and its preparation side
Method, patent of invention（CN106633990A）It is by fungi based biomass packaging material of major ingredient and preparation method thereof of maize straw
Row patent is all solely using a kind of material as major ingredient, and base-material requires relatively complicated, and not to be related to fire-resistance resistance to for only excellent mechanical property
It is aqueous；Patent of invention (CN104025909A) it is a kind of inoculation edible fungus species culture degradable package material method, have compared with
Good fire line, but without the advantage in weight.
Invention content
In order to solve the above technical problem, the present invention provides a kind of degradable package material is prepared using Schizophyllum commune for Mycelium
Method, the present invention is one kind based on agricultural crop straw, and apple sawdust, the Schizophyllum commune for Mycelium supplemented by wheat shell prepares packaging
The technology of material.
The present invention is achieved through the following technical solutions, and the present invention, which provides a kind of prepared using Schizophyllum commune for Mycelium, to drop
The method for solving packaging material, includes the following steps：
S101：Compost prepares：By maize straw, wheat stalk, apple sawdust, wheat shell, cotton seed hulls, lime, gypsum mixing
Uniformly；1.25 ~ 1.50 times of water of the dry weight that adds materials into compost, mixing, water content in regulation culture raw material to 55%-
60%, it is then charged into sterilizing bag sterilizing；
S102：Strain preculture：Schizophyllum commune liquid spawn is inoculated in the bacterium bag being cooled to room temperature, lid culture is covered；
S103：Mold filling culture：The spectacle case that aseptically material for covering with Schizophyllum commune for Mycelium filling has been sterilized is die-filling
In tool, continue to cultivate after being pressed and sealed；
S104：Sample drying：After Schizophyllum commune for Mycelium is tied again in the material in mold, sample is taken out of mold
Go out the dry water content to sample and be less than 20%, you can obtains degradable package material.
The present invention is with plant fiber（Wheat shell, wheat stalk, maize straw etc.）And apple branch is primary raw material, system
It is standby a kind of novel based on the mycelial degradable package material of macro fungi；Raw material extensive, preparation process with raw material sources
Simply, of low cost, the advantages that Life cycle is pollution-free, belong to environmental type packaging material, and can make full use of
Agricultural straw resource has wide promotion prospect.
Schizophyllum commune is a kind of edible medicinal macro fungi, is widely distributed in all over the world, in food industry, medical and health, bioid
Learn etc. is widely used.Schizophyllum commune includes mycelium and fructification two parts composition, and fructification edible value is high, the tender taste of matter
U.S. has special strong fragrance, mild-natured, has strengthening by means of tonics, sedation, for high-grade edible and medical fungi.The bacterium mycelium contains
More strongly active cellulase, and can generate malic acid, when mycelia submerged fermentation, can generate a large amount of organic acids, can also generate growth-promoting
Plain heteroauxin, using its mycelium make packaging material there is not yet.
Preferably, in S101, material is added and is calculated by weight as：20~25 parts of maize straws, 20~25 portions of wheats
Stalk, 10~15 parts of apple sawdusts, 5~10 parts of wheat shells, 5~10 parts of cotton seed hulls, 1~3 part of lime, 1~2 part of gypsum.
Preferably, in S101, be added material be calculated by weight as 25 parts of maize straws, 25 parts of wheat stalks, 15 parts
Apple sawdust, 10 parts of wheat shells, 10 parts of cotton seed hulls, 3 parts of lime, 2 parts of gypsum.
Preferably, in S101, sterilizing bag in autoclaving equipment in sterilizing 2 hours.
Preferably, in S102, condition of culture is：It is 20 DEG C -25 DEG C of temperature, envionmental humidity 60%, draughty
It is cultivated -15 days 10 days in dark constant incubator, until covering with mycelium on material.
Preferably, in S103, condition of culture is：20 DEG C of temperature, humidity 60% cultivate 5 in dark constant incubator
It.
Preferably, S104：Sample takes out dry under the conditions of 60 DEG C out of mold.
Beneficial effects of the present invention are：
1）The present invention produces one kind using crop material as primary raw material, and apple sawdust, wheat shell is auxiliary material, with schizophyllum commune bacterium
Filament is the environment-friendly package material of binder, you can solves the problems, such as existing packaging non-degradable, can also preferably handle work
Object stalk and fruit tree.
2）The raw material of the present invention is with raw material sources are extensive, preparation process is simple, of low cost, Life cycle is pollution-free
The advantages that, belong to environmental type packaging material, and agricultural straw resource can be made full use of, before there is wide popularization
Scape.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is further described with reference to specific embodiment.
Embodiment 1, a method of degradable package material being prepared using Schizophyllum commune for Mycelium, is included the following steps：
S101：Compost prepares：By 25 parts of maize straws, 25 parts of wheat stalks, 15 parts of apple sawdusts, 10 parts of wheat shells, 10 parts
Cotton seed hulls, 3 parts of lime, 2 parts of gypsum are uniformly mixed；1.25 times of the dry weight that adds materials into compost ~ 1.50 times of water, mixing,
Water content in regulation culture raw material is then charged into sterilizing bag to 55%-60%, in sterilizing 2 hours in autoclaving equipment.
S102：Strain preculture：Schizophyllum commune liquid spawn is inoculated in the bacterium bag being cooled to room temperature, lid is covered, is put
It is placed in 20 DEG C -25 DEG C of temperature, envionmental humidity 60% or so, draughty dark constant incubator and cultivates 10 days -15
It, until covering with mycelium on material.
S103：Mold filling culture：The material for covering with Schizophyllum commune for Mycelium is aseptically inserted to the spectacle case sterilized
In die-filling tool, it is positioned over 20 DEG C of temperature after being pressed and sealed, humidity 60% is cultivated 5 days in dark constant incubator.
S104：Sample drying：After Schizophyllum commune for Mycelium is tied again in the material in mold, by sample from mold
Interior taking-up, which is dried under the conditions of 60 DEG C to the water content of sample, is less than 20%, you can obtains with plant fiber（Wheat shell, Wheat Straw
Stalk, maize straw etc.）And the fungi based biomass packaging material that apple branch is primary raw material.
Embodiment 2, a method of degradable package material being prepared using Schizophyllum commune for Mycelium, is included the following steps：
S101：Compost prepares：By 20 parts of maize straws, 20 parts of wheat stalks, 10 parts of apple sawdusts, 5 parts of wheat shells, 5 portions of cottons
Seed shell, 1 part of lime, 1 part of gypsum are uniformly mixed；1.25 times of the dry weight that adds materials into compost ~ 1.50 times of water, mixing are adjusted
Water content in control culture raw material is then charged into sterilizing bag, in sterilizing 2 hours in autoclaving equipment to 55%-60%.
S102：Strain preculture：Schizophyllum commune liquid spawn is inoculated in the bacterium bag being cooled to room temperature, lid is covered, is put
It is placed in 20 DEG C -25 DEG C of temperature, envionmental humidity 60% or so, draughty dark constant incubator and cultivates 10 days -15
It, until covering with mycelium on material.
S103：Mold filling culture：The material for covering with Schizophyllum commune for Mycelium is aseptically inserted to the spectacle case sterilized
In die-filling tool, it is positioned over 20 DEG C of temperature after being pressed and sealed, humidity 60% is cultivated 5 days in dark constant incubator.
S104：Sample drying：After Schizophyllum commune for Mycelium is tied again in the material in mold, by sample from mold
Interior taking-up, which is dried under the conditions of 60 DEG C to the water content of sample, is less than 20%, you can obtains with plant fiber（Wheat shell, Wheat Straw
Stalk, maize straw etc.）And the fungi based biomass packaging material that apple branch is primary raw material.
Embodiment 3, a method of degradable package material being prepared using Schizophyllum commune for Mycelium, is included the following steps：
S101：Compost prepares：By 22 parts of maize straws, 22 parts of wheat stalks, 12 parts of apple sawdusts, 8 parts of wheat shells, 8 portions of cottons
Seed shell, 2 parts of lime, 2 parts of gypsum are uniformly mixed；1.25 times of the dry weight that adds materials into compost ~ 1.50 times of water, mixing are adjusted
Water content in control culture raw material is then charged into sterilizing bag, in sterilizing 2 hours in autoclaving equipment to 55%-60%.
S102：Strain preculture：Schizophyllum commune liquid spawn is inoculated in the bacterium bag being cooled to room temperature, lid is covered, is put
It is placed in 20 DEG C -25 DEG C of temperature, envionmental humidity 60% or so, draughty dark constant incubator and cultivates 10 days -15
It, until covering with mycelium on material.
S103：Mold filling culture：The material for covering with Schizophyllum commune for Mycelium is aseptically inserted to the spectacle case sterilized
In die-filling tool, it is positioned over 20 DEG C of temperature after being pressed and sealed, humidity 60% is cultivated 5 days in dark constant incubator.
S104：Sample drying：After Schizophyllum commune for Mycelium is tied again in the material in mold, by sample from mold
Interior taking-up, which is dried under the conditions of 60 DEG C to the water content of sample, is less than 20%, you can obtains with plant fiber（Wheat shell, Wheat Straw
Stalk, maize straw etc.）And the fungi based biomass packaging material that apple branch is primary raw material.
Certainly, above description is also not limited to the example above, technical characteristic of the present invention without description can by or
It is realized using the prior art, details are not described herein；It is not to this that above example, which is only used to illustrate the technical scheme of the present invention,
The limitation of invention, describes the invention in detail with reference to preferred embodiment, and those skilled in the art should
Understand, the variations, modifications, additions or substitutions that those skilled in the art are made in the essential scope of the present invention
Without departure from spirit of the invention, it should also belong to the claims of the present invention.
Claims (7)
1. a kind of method preparing degradable package material using Schizophyllum commune for Mycelium, it is characterised in that：Include the following steps：
S101：Compost prepares：By maize straw, wheat stalk, apple sawdust, wheat shell, cotton seed hulls, lime, gypsum mixing
Uniformly；1.25 ~ 1.50 times of water of the dry weight that adds materials into compost, mixing, water content in regulation culture raw material to 55%-
60%, it is then charged into sterilizing bag sterilizing；
S102：Strain preculture：Schizophyllum commune liquid spawn is inoculated in the bacterium bag being cooled to room temperature, lid culture is covered；
S103：Mold filling culture：The spectacle case that aseptically material for covering with Schizophyllum commune for Mycelium filling has been sterilized is die-filling
In tool, continue to cultivate after being pressed and sealed；
S104：Sample drying：After Schizophyllum commune for Mycelium is tied again in the material in mold, sample is taken out of mold
Go out the dry water content to sample and be less than 20%, you can obtains degradable package material.
2. a kind of method preparing degradable package material using Schizophyllum commune for Mycelium according to claim 1, feature
It is：In S101, material is added and is calculated by weight as：20~25 parts of maize straws, 20~25 parts of wheat stalks, 10~15 parts
Apple sawdust, 5~10 parts of wheat shells, 5~10 parts of cotton seed hulls, 1~3 part of lime, 1~2 part of gypsum.
3. a kind of method preparing degradable package material using Schizophyllum commune for Mycelium according to claim 2, feature
It is：In S101, material is added and is calculated by weight as 25 parts of maize straws, 25 parts of wheat stalks, 15 parts of apple sawdusts, 10 parts
Wheat shell, 10 parts of cotton seed hulls, 3 parts of lime, 2 parts of gypsum.
4. a kind of method preparing degradable package material using Schizophyllum commune for Mycelium according to claim 1, feature
It is：In S101, sterilizing bag in autoclaving equipment in sterilizing 2 hours.
5. a kind of method preparing degradable package material using Schizophyllum commune for Mycelium according to claim 1, feature
It is：In S102, condition of culture is：20 DEG C -25 DEG C of temperature, envionmental humidity 60%, draughty dark constant temperature incubation
It is cultivated -15 days 10 days in case, until covering with mycelium on material.
6. a kind of method preparing degradable package material using Schizophyllum commune for Mycelium according to claim 1, feature
It is：In S103, condition of culture is：20 DEG C of temperature, humidity 60% are cultivated 5 days in dark constant incubator.
7. a kind of method preparing degradable package material using Schizophyllum commune for Mycelium according to claim 1, feature
It is：S104：Sample takes out dry under the conditions of 60 DEG C out of mold.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934760A (en) * | 2018-07-11 | 2018-12-07 | 鲁东大学 | A method of degradable package material is prepared using Schizophyllum commune for Mycelium |
| CN109897394A (en) * | 2019-01-22 | 2019-06-18 | 鲁东大学 | A method of degradable package material is prepared using Schizophyllum commune for Mycelium |
| CN110655801A (en) * | 2019-11-18 | 2020-01-07 | 常熟理工学院 | Bio-based composite material and preparation method thereof |
| CN112679978A (en) * | 2020-12-24 | 2021-04-20 | 李红霞 | Preparation method of antibacterial biomass packaging material |
| CN113016595A (en) * | 2019-12-25 | 2021-06-25 | 深圳市南理工技术转移中心有限公司 | Degradable planting block for promoting crop growth for water planting and manufacturing method thereof |
| CN114196554A (en) * | 2022-01-27 | 2022-03-18 | 山东农业大学 | Schizophyllum commune and application thereof in degradation of waste branches of orchard |
| WO2023123737A1 (en) * | 2021-12-31 | 2023-07-06 | 深圳市泽青源科技开发服务有限公司 | Mycelium packaging material having anti-counterfeiting function and method for using same |
| US11920126B2 (en) | 2018-03-28 | 2024-03-05 | Ecovative Design Llc | Bio-manufacturing process |
| US11932584B2 (en) | 2006-12-15 | 2024-03-19 | Ecovative Design Llc | Method of forming a mycological product |
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| CN105440719A (en) * | 2015-12-15 | 2016-03-30 | 郭锐 | Method for preparing buffer material from plant straws |
| CA2894888A1 (en) * | 2015-06-22 | 2016-12-22 | Leah A. Perry | Myco-active biodegradable garbage bag |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CA2894888A1 (en) * | 2015-06-22 | 2016-12-22 | Leah A. Perry | Myco-active biodegradable garbage bag |
| CN105440719A (en) * | 2015-12-15 | 2016-03-30 | 郭锐 | Method for preparing buffer material from plant straws |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11932584B2 (en) | 2006-12-15 | 2024-03-19 | Ecovative Design Llc | Method of forming a mycological product |
| US11920126B2 (en) | 2018-03-28 | 2024-03-05 | Ecovative Design Llc | Bio-manufacturing process |
| CN108934760A (en) * | 2018-07-11 | 2018-12-07 | 鲁东大学 | A method of degradable package material is prepared using Schizophyllum commune for Mycelium |
| CN109897394A (en) * | 2019-01-22 | 2019-06-18 | 鲁东大学 | A method of degradable package material is prepared using Schizophyllum commune for Mycelium |
| CN110655801A (en) * | 2019-11-18 | 2020-01-07 | 常熟理工学院 | Bio-based composite material and preparation method thereof |
| CN113016595A (en) * | 2019-12-25 | 2021-06-25 | 深圳市南理工技术转移中心有限公司 | Degradable planting block for promoting crop growth for water planting and manufacturing method thereof |
| CN113016595B (en) * | 2019-12-25 | 2022-08-09 | 深圳市南理工技术转移中心有限公司 | Degradable planting block for promoting crop growth for water planting and manufacturing method thereof |
| CN112679978A (en) * | 2020-12-24 | 2021-04-20 | 李红霞 | Preparation method of antibacterial biomass packaging material |
| WO2023123737A1 (en) * | 2021-12-31 | 2023-07-06 | 深圳市泽青源科技开发服务有限公司 | Mycelium packaging material having anti-counterfeiting function and method for using same |
| CN114196554A (en) * | 2022-01-27 | 2022-03-18 | 山东农业大学 | Schizophyllum commune and application thereof in degradation of waste branches of orchard |
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Application publication date: 20181106 |
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