CN102311279A - A kind of is culture bag major ingredient edible fungus culturing production technology with the residue of soya - Google Patents
A kind of is culture bag major ingredient edible fungus culturing production technology with the residue of soya Download PDFInfo
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- CN102311279A CN102311279A CN2010102140179A CN201010214017A CN102311279A CN 102311279 A CN102311279 A CN 102311279A CN 2010102140179 A CN2010102140179 A CN 2010102140179A CN 201010214017 A CN201010214017 A CN 201010214017A CN 102311279 A CN102311279 A CN 102311279A
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of is culture bag major ingredient edible fungus culturing production technology with the residue of soya, belongs to the technical field of waste resource regenerated using, and providing a kind of is culture bag major ingredient edible fungus culturing production technology with the residue of soya.This techniques make use soy sauce slag carries out Edible Fungi for the culture bag major ingredient.Its main process: at first the slant strains of preserving is inoculated on the PDA substratum that contains 20% soy sauce slag (moisture 78%); Cultivate 6d for 26 ℃, insert by certain inoculum size then and carry out the cultivation of shaking table liquid seeds, 158rpm in the liquid nutrient medium that contains the soy sauce slag; 26 ℃; 4d inserts in the solidified soy sauce slag for cultivating Bag Material proper temperature cultivating and producing fruit body of edible fungi as producing the inoculum size of bacterial classification with 10%.
Description
Technical field:
The invention belongs to the technical field of waste resource regenerated using, particularly residue of soya waste resource regenerated using is specifically related to utilize the residue of soya waste resource to be main raw material edible mushrooms culture technique.
Background technology:
Residue of soya is a big integral part of brewageing waste residue, is meant through hot-water soak sauce unstrained spirits, drenches remaining residue after the soy sauce.Wherein crude protein (butt) is 25-27%, and full nitrogen (butt) is 4.0-4.4%, and thick starch (butt) is 5.2%, moisture 80%.China has the residue of soya more than 2,700,000 tons every year approximately.The residue of soya water cut is big, and protein contnt is high, and it is rotten very easily to go mouldy, and gives off a peculiar smell; Therefore the enterprise of ordinary production soy sauce must handle every day at once, otherwise environmental pollution is extremely serious, particularly summer; Handle untimelyly, can produce strong pungent smell, cause that fly maggot grows.Be also to contain a large amount of crude fat and salinity in the brewing residue of soya of protein raw materials with the soybean, it directly as the farming fertilizer or the feed of breeding fish, can be made the soil salinization, makes fish dead, have a strong impact on water quality, environmental protection again.For a long time, receive the restriction of economy, science and technology, many areas all are used as residue of soya as industrial refuse and are carried out landfill disposal and cause waste.Along with the intensified competition in market, the class of soy sauce product is increasingly high, but the profit of enterprise is reduction trend.High-grade product much is to be protein raw materials with the soybean, produces the higher sub product of value capable of using.If can realize recycling to it, will bring fair margin of profit for enterprise.Simultaneously, the exploitation residue of soya is realized the recycling of resource, can effectively prevent resource loss, avoids environmental pollution, meets current country and greatly develops recycling economy, the poolicy orientation of building a conservation-minded society.This shows, residue of soya is researched and developed, not only can utilize its abundant effective constituent, or the demand of enterprise and social development.People once attempted it directly as feed.But the residue of soya animal palatability that does not have to handle is poor, and wherein salt content is too high, feeds for a long time or once feeds amount too much, very easily causes salt poisoning, and the lighter's feed falls sharply, and loses weight, and is heavy then animal dead often has the report that causes animal dead because of residue of soya.And be to contain a large amount of soya-bean oil in the residue of soya of raw material with the soybean; And unsaturated fatty acid content is high in the soya-bean oil, in depositing process, very easily is oxidized into lipoperoxide, and this is the material that a kind of character is active, oxidisability is strong; Many unsaturated fatty acidss on the microbial film are very responsive to its attack; And in case reaction starts, will go on the chain reaction mode, cause the generation of a large amount of lipoperoxides.These superoxide are fragmented into the aldehydes molecules of different sizes, and pair cell produces very strong toxicity, biomembranous structure is changed, and then influence its function, for example make flowability decline, permeability changes, the film transportation of film disorderly or the like.Therefore, only have the small portion residue of soya to undersell as feed at present, major part is not utilized and is wasted.
China is Edible Fungi big country, and about 1,400 ten thousand tons of edible mushrooms YO has accounted for more than 70% of world's gross output, exports goods and earns foreign currency 1,200,000,000 dollars last year.It is bigger to the raw materials requirement amount to cultivate edible mushrooms, and main raw material is some agricultural byproducts and trees etc. at present.To save a lot of resources if utilize residue of soya to be used for culturing edible fungus, the protection environment, significant to the human kind sustainable development strategy.
Summary of the invention:
The objective of the invention is to handle waste and the environmental pollution problems that exists to this reproducible utilization resource of present residue of soya, provide and utilize residue of soya to prepare the method for the cultivating materials of edible mushrooms for main raw material, this method can not only effectively fully utilize discarded residue of soya; Reduce environmental pollution, and adopt the liquid spawn of residue of soya domestication, reduce to pollute and shortened the production of hybrid seeds cycle simultaneously; Edible mushrooms is in the cultivation process of major ingredient at residue of soya; Mycelial growth is evenly vigorous, and culture cycle is short, and fruiting early; Change of tide is fast, and the edible fungi nutrition of producing is worth the edible mushrooms that reaches with obtaining as main material production with trees, cotton seed hulls etc. and is equal to.Gather and contain a large amount of true albumen, active polysaccharide in the cultivation foodstuff behind the edible mushrooms.Crude fiber content and traditional cotton seed hulls, straw, weed tree sawdust etc. are compared greatly for the foodstuff of main raw material and are reduced, and can be used as fodder additives and are used for Production of Livestock and Poultry and also can be used as the cultivation that organic fertilizer is used for famous and precious plant.
Concrete scheme of the present invention is following:
Edible fungi liquid strain according to the invention is made and cultivation major ingredient residue of soya is to drench the residue that remaining fresh nothing is gone mouldy after the soy sauce; Wherein crude protein (butt) is 20-27%, and full nitrogen (butt) is 3.5-4.5%, and thick starch (butt) is 5-7%; Moisture 70-80%, salt content 1.5-3%.
Edible fungi liquid strain according to the invention at first carries out 25 ℃ of cultivation 7-10d before making inoculation on the test tube slant, cultivate the slant medium that uses and be PDA substratum (containing sucrose 2%, agar 2%)+20% soy sauce slag; From cultivate sophisticated inclined-plane, insert in the level liquid seed culture medium (300mL triangular flask loading amount 40mL) with two ferfas pieces under the inoculation shovel shovel then, the bacterium piece size is as the criterion with inoculation shovel size, is generally 0.5cm * 0.5cm size; The liquid culture based formulas is that (edible mushrooms all can grow in residue of soya concentration 0-40% range of concentrations fresh residue of soya 20%; The best residue of soya concentration of level liquid seed culture medium is about 20%), Semen Maydis powder 1%, glucose 1%; Wheat bran 1%; Straw powder 1%, sal epsom 0.02%, pH 5.5-5.8.Postvaccinal liquid culture is shaken bottle be placed on 25-28 ℃, cultivate 5-6d in the constant temperature oscillator of 158r/min.After the acclimation shaking culture through 1-2d in residue of soya level liquid seed culture medium; The quantity of the bacterium ball in the substratum increases gradually; The bacterium ball is tiny grain of rice shape, along with the propelling of incubation time, and the level liquid seed culture medium clarification that becomes gradually behind the 5-6d; Thickness, level liquid seed are cultivates maturation.Insert in the secondary liquid seed culture medium by 10% of inoculum size, the secondary seed medium prescription is fresh residue of soya 35%, Semen Maydis powder 1% again; Glucose 1%, wheat bran 1%, straw powder 1%; Sal epsom 0.02%, pH 5.5-5.8 shakes bottle with postvaccinal liquid culture and is placed on 25-28 ℃; Cultivate 4d in the constant temperature oscillator of 158r/min and disappear to the fibrous material in the substratum, only remaining has heavy-gravity bacterium ball liquid.
According to the invention is that culture bag major ingredient edible fungus culturing process is to get to cultivate sophisticated liquid edible bacterial classification and in Bechtop, insert and sterilized and be cooled in the solid state cultivation bag about 30 ℃ with the residue of soya; Culture medium prescription is fresh residue of soya 80%, straw powder 13%, Semen Maydis powder 3%, wheat bran 3%, rice bran 1% in the solid state cultivation bag; PH 5.5-5.8; Inoculum size is controlled at 10%, and promptly 100 gram solid mediums insert the 10ml liquid spawn.Postvaccinal culture bag is placed on 25-28 ℃; Cultivate in the dark chamber of humidity about 65%, treat that mycelia is covered with, mycelium differentiation kink forms fruit body primordium; Can see the projection of adularescent, yellow or chocolate; Want fully printing opacity ventilation at once this moment, create day and night temperature, keep relative air humidity 90-95%.Can spray thin water in media surface, keep not ponding of charge level, along with the overstriking of growing tall of stem, cap is grown up, and can spray water every day this moment 3-4 time, up to gathering.
According to the invention is that culture bag major ingredient edible fungus culturing process is cultivated and management parameters is (1) temperature with the residue of soya: hypha of Pleurotus ostreatus growth optimum temperuture is 25 ℃-28 ℃, and it is 15 ℃-20 ℃ that sporophore forms optimum temperuture.(2) humidity: the vegetative stage atmospheric moisture is 70% to be advisable, and is main with dark, strong mycelial growth.(3) if local mycelia does not sprout, available aseptic inoculation shovel is played the ventilation of several holes, also can spray the alcohol mixeding liquid of 10% sugar and 20%, impels mycelium germination.
Contain more protein and abundant active polysaccharide in the cultivation foodstuff after fruiting is gathered according to the invention; Wherein crude protein content is about 30% (in butt); True protein content accounts for more than 90% of crude protein content; Crude fiber content and traditional cotton seed hulls, straw, weed tree sawdust etc. are compared greatly for the foodstuff of main raw material and are reduced, and can be used as fodder additives and are used for Production of Livestock and Poultry and also can be used as the cultivation that organic fertilizer is used for famous and precious plant.
Embodiment:
Embodiment 1 (is example with the mushroom cultivation)
1, liquid seeds preparation
(1) level liquid seed culture based formulas: fresh residue of soya 20%, Semen Maydis powder 1%, glucose 1%, wheat bran 1%, straw powder 1%, sal epsom 0.02%, pH 5.5-5.8.
(2) secondary liquid seed culture medium prescription: fresh residue of soya 35%, Semen Maydis powder 1%, glucose 1%, wheat bran 1%, straw powder 1%, sal epsom 0.02%, pH 6.0-7.0.
(3) sterilising method is feeded back 121 ℃, and 0.1MP sterilization sterilization in 30 minutes postcooling to 30 is ℃ subsequent use.
(4) inoculation method level liquid seed inserts slant strains 2-3 piece (every be as the criterion with inoculation shovel size, be generally 0.5cm * 0.5cm size), operates on the aseptic technique platform and carries out.The secondary liquid seeds has been cultivated sophisticated level liquid seed by 10% inoculum size inoculation
(5) cultivation is shaken bottle with postvaccinal liquid culture and is placed on 25-28 ℃, cultivates 5-6d in the constant temperature oscillator of 158r/min.After the acclimation shaking culture through 1-2d in residue of soya level liquid seed culture medium; The quantity of the bacterium ball in the substratum increases gradually; The bacterium ball is tiny grain of rice shape; Along with the propelling of incubation time, become gradually clarification, thickness of level liquid seed culture medium behind the 5-6d, this moment the level liquid seed be cultivate ripe.In the 10% access secondary liquid seed culture medium by inoculum size, postvaccinal liquid culture is shaken bottle be placed on 25-28 ℃ again, cultivate 4d in the constant temperature oscillator of 158r/min and disappear to the fibrous material in the substratum, only remaining has heavy-gravity bacterium ball liquid.
2, with the residue of soya be culture bag major ingredient edible fungus culturing process
(1) fill a prescription fresh residue of soya 80%, straw powder 13%, Semen Maydis powder 3%, wheat bran 3%, rice bran 1% of culture material, pH 5.5-5.8.
(2) spice takes by weighing culture material according to the above ratio, is adjusted to suitable water cut.Notice that moisture can not once add, add in batches, and mix evenly.
(3) adjust pH makes the pH value at 5.5-5.8.Alkali is regulated with Hydrocerol A partially; Meta-acid is regulated with lime powder.Mode with spraying adds, and turns while spraying.
(4) after charging, tying material are mixed, rapid packing.With the acrylic plastering wound packages material of 23cm * 55cm, limit rim compacting treats that culture material installs, and with the flat charge level of hand, is expecting central punch with the punching rod again.The two ends of bag can add that tampon seals or tie with the rope reflexed with neck ring.Pocket requires the tight middle pine in two, along the tight inwall pine of bag wall.
(5) after the sterilization material installs, should in time sterilize, prevent that matrix acid from becoming.High pressure steam sterilization, sterilization 1-2h, temperature is about 121 ℃.
(6) after the cooling sterilization finishes, take out pocket and place transfer room.Be cooled to about 30 ℃.
(7) 100 milliliters of syringes are got in inoculation; Remove syringe needle and change an internal diameter 1-2 millimeter, the stainless steel of long 100-200 millimeter or aluminum pipe are processed a bacterial classification inoculation device, clean inoculator and bind up with gauze the dress plastics bag before using; Through the high-temperature sterilization postcooling; Extracting liquid spawn can inoculate, and can open the inoculation of bag or bottleneck, also can directly syringe needle be inserted (earlier with 75% alcohol or iodine disinfection ingress) injection inoculation in seed bottle (bag) sealing film; Can paste surgical adhesive after the inoculation and seal, also can dry lime seal.Inoculum size is 10%.
(8) send out the bacterium cultivation postvaccinal culture bag is placed on 25-28 ℃; Cultivate in the dark chamber of humidity about 65%, treat that mycelia is covered with, mycelium differentiation kink forms fruit body primordium; Can see the projection of adularescent, yellow or chocolate; Want fully printing opacity ventilation at once this moment, create day and night temperature, keep relative air humidity 90-95%.Can spray thin water in media surface, keep not ponding of charge level, along with the overstriking of growing tall of stem, cap is grown up, and can spray water every day this moment 3-4 time, up to gathering.
Cultivate and management parameters:
A, temperature: hypha of Pleurotus ostreatus growth optimum temperuture is 25 ℃-28 ℃, and it is 15 ℃-20 ℃ that sporophore forms optimum temperuture.
B, humidity: the vegetative stage atmospheric moisture is 70% to be advisable, and is main with dark, strong mycelial growth.
C, if local mycelia does not sprout, available aseptic inoculation shovel is played the ventilation of several holes, also can spray 10% sugared and 20% alcohol mixeding liquid, impels mycelium germination.
(9) cultivate foodstuff after oven dry, can be directly as fodder additives or as organic fertilizer.
Claims (6)
1. one kind is culture bag major ingredient edible fungus culturing production technology with the residue of soya; Be primarily characterized in that with this reproducible utilization resource of residue of soya be the main raw material culturing edible fungus; Can effectively fully utilize discarded residue of soya, reduce environmental pollution, adopt the liquid spawn of residue of soya domestication simultaneously; Shortened the production of hybrid seeds cycle; Edible mushrooms is that mycelial growth is evenly vigorous in the cultivation process of major ingredient at residue of soya, and the edible fungi nutrition value of producing reaches and is equal to edible mushroomss that production obtains as major ingredient such as trees, cotton seed hullss.Cultivate foodstuff and contain abundant true albumen, active polysaccharide etc., robust fibre and traditional foodstuff ratio content mutually significantly reduce, can be directly as fodder additives or organic fertilizer use.
2. according to claim 1 a kind of be culture bag major ingredient edible fungus culturing production technology with the residue of soya; It is characterized in that it is fresh residue of soya 20% that said edible fungi liquid strain is made the level liquid seed culture medium, Semen Maydis powder 1%, glucose 1%; Wheat bran 1%; Straw powder 1%, sal epsom 0.02%, pH 5.5-5.8; The secondary liquid seed culture medium is fresh residue of soya 35%, Semen Maydis powder 1%, glucose 1%, wheat bran 1%, straw powder 1%, sal epsom 0.02%, pH 5.5-5.8.
3. described according to claim 1 and claim 2 is culture bag major ingredient edible fungus culturing production technology with the residue of soya; It is characterized in that the edible fungi liquid strain making; The edible mushrooms slant strains is tamed 1-2d in residue of soya level liquid seed culture medium; Culture temperature 25-28 ℃, shaking speed 158r/min, it is ripe to continue to be cultured to behind the 5-6d level liquid seed culture.The secondary liquid seeds is cultivated, inoculum size 10%, and culture temperature 25-28 ℃, shaking speed 158r/min cultivates 4d and disappears to the fibrous material in the substratum, and only remaining has heavy-gravity bacterium ball liquid, and liquid spawn is ripe.
4. according to claim 1 a kind of be culture bag major ingredient edible fungus culturing production technology with the residue of soya, plant formulation is fresh residue of soya 80%, straw powder 13%, Semen Maydis powder 3%, wheat bran 3%, rice bran 1%, pH 5.5-5.8.
According to claim 1,2,3 and 4 described a kind of be culture bag major ingredient edible fungus culturing production technology with the residue of soya; Be primarily characterized in that the edible fungus culturing process is cultivated and management parameters is (1) temperature: the mycelial growth optimum temperuture is 25 ℃-28 ℃, and it is 15 ℃-20 ℃ that sporophore forms optimum temperuture.(2) humidity: the vegetative stage atmospheric moisture is 70% to be advisable, and is main with dark, favourable mycelial growth.(3) if local mycelia does not sprout, available aseptic inoculation shovel is played the ventilation of several holes, also can spray the alcohol mixeding liquid of 10% sugar and 20%, impels mycelium germination.
According to claim 1,2,3,4 and 5 described a kind of be culture bag major ingredient edible fungus culturing production technology with the residue of soya; Be primarily characterized in that crude protein content is about 30% (in butt) in the cultivation foodstuff after fruiting is gathered; True protein content accounts for more than 90% of crude protein content; Crude fiber content and traditional cotton seed hulls, straw, weed tree sawdust etc. are compared greatly for the foodstuff of main raw material and are reduced, and can be used as fodder additives and are used for Production of Livestock and Poultry and also can be used as the cultivation that organic fertilizer is used for famous and precious plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010214017.9A CN102311279B (en) | 2010-06-30 | 2010-06-30 | Production technique for cultivating edible funguses by taking soy sauce residues as major ingredient of cultivation bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104193484A (en) * | 2014-07-24 | 2014-12-10 | 安徽天都灵芝制品公司 | Straw mushroom culture medium containing sauce residue and preparation method of straw mushroom culture medium |
| CN104326841A (en) * | 2014-11-24 | 2015-02-04 | 王惠莹 | Pleurotus ostreatus culture medium and preparation method thereof |
| CN105009929A (en) * | 2014-04-21 | 2015-11-04 | 浙江科技学院 | Grifold frondosa cultivation production technology with salvia miltiorrhiza dregs as main materials of cultivation bag |
| CN106957852A (en) * | 2017-03-29 | 2017-07-18 | 上海市农业科学院 | A kind of asparagus method for transformation using the grain of rice as culture matrix |
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2010
- 2010-06-30 CN CN201010214017.9A patent/CN102311279B/en not_active Expired - Fee Related
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| 徐生俊等: "酿造废糟渣替代金针菇常规栽培料试验", 《上海调味品》 * |
| 戴德慧等: "酱渣食用菌发酵生产功能性饲料的研究", 《浙江农业科学》 * |
| 暴增海等: "《食用菌栽培学》", 31 January 2010 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009929A (en) * | 2014-04-21 | 2015-11-04 | 浙江科技学院 | Grifold frondosa cultivation production technology with salvia miltiorrhiza dregs as main materials of cultivation bag |
| CN104193484A (en) * | 2014-07-24 | 2014-12-10 | 安徽天都灵芝制品公司 | Straw mushroom culture medium containing sauce residue and preparation method of straw mushroom culture medium |
| CN104326841A (en) * | 2014-11-24 | 2015-02-04 | 王惠莹 | Pleurotus ostreatus culture medium and preparation method thereof |
| CN106957852A (en) * | 2017-03-29 | 2017-07-18 | 上海市农业科学院 | A kind of asparagus method for transformation using the grain of rice as culture matrix |
| CN106957852B (en) * | 2017-03-29 | 2020-11-06 | 上海市农业科学院 | Flammulina velutipes transformation method using rice grains as culture medium |
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| CN102311279B (en) | 2015-06-17 |
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