CN105009929A - Grifold frondosa cultivation production technology with salvia miltiorrhiza dregs as main materials of cultivation bag - Google Patents
Grifold frondosa cultivation production technology with salvia miltiorrhiza dregs as main materials of cultivation bag Download PDFInfo
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Abstract
The present invention relates to the technical field of waste resources recycling and provides a grifold frondosa cultivation production technology with salvia miltiorrhiza dregs as main materials of a cultivation bag. Aimed at the waste and environmental pollution problems existent in the processing of reusable resources, namely the salvia miltiorrhiza dregs, at present, a method for preparing cultivation materials of grifold frondosa by using the salvia miltiorrhiza dregs as the main materials is provided. The method can not only effectively comprehensively utilize waste salvia miltiorrhiza dregs and alleviate environmental pollution, but also shorten seed producing period while alleviating environmental pollution by using a liquid strain acclimated by the salvia miltiorrhiza dregs. During the process for cultivating the grifold frondosa by taking the salvia miltiorrhiza dregs as the main materials, mycelia grow uniformly and vigorously, the cultivation period is short, mushrooms emerge early, tide turns quickly, and the nutritional value of the produced grifold frondosa is equal to that of grifold frondosa produced with trees, cottonseed hulls and the like as main materials. A cultivation food material contain more true proteins and activated polysaccharides, and the content of coarse fibers is greatly reduced compared to that in a conventional food material, so that the food material can be directly used as a feed additive or an organic fertilizer.
Description
Technical field
The invention belongs to the technical field of waste resource regeneration, particularly the regeneration of red sage root water extraction dregs of a decoction waste resource, be specifically related to utilize red sage root dregs of a decoction waste resource for primary raw material grifola frondosus cultivation production technology.
Background technology
The red sage root (Salvia miltiorrhizaBge.) is the herbaceos perennial that Lamiaceae Salvia belongs to, and salviamiltiorrhizabung is the dry root and rhizome of the plant red sage root.The functional component of the current utilization in salviamiltiorrhizabung is water-soluble danshinolic acid class and fat-soluble tanshinone.Water-soluble danshinolic acid class, mainly based on tanshin polyphenolic acid B, also comprises salviandic acid A, C, D, E, F, G, protocatechualdehyde, protocatechuic acid etc.Tanshin polyphenolic acid B has very strong antioxidation, experiment in vivo and vitro proves, tanshin polyphenolic acid B energy scavenging activated oxygen, anti-lipid peroxidation react, and its action intensity, higher than vitamin C, vitamin E, mannitol, is one of natural products that known at present antioxidation is the strongest.Its main pharmacological has cardiovascu-lar effects, anti-infectious function, antitumor action, to effects such as neurocyte protection effect, sex hormone sample activity.China's red sage root Chinese patent drug preparation is normal adopts hot water extraction's red sage root, and its water-soluble extractives and other drug are mixed and made into red sage root Chinese patent drug, are used for the treatment of the treatment of the disease such as coronary heart diseases and angina pectoris, skin disease.Therefore after having a large amount of red sage root decoctings to extract in the process of producing, solid slag remains.Because water content is comparatively large, its moisture, up to more than 80%, very easily addles, and its taste is smelly different, even more serious in summer, is difficult to store.Or mishandling, very easily to environment and going mouldy, cause producing medicine and plant environment pollutes.But containing outside a large amount of red sage root fat-soluble active ingredient dihydrotanshinone, Cryptotanshinone, Tanshinone I and Tanshinone I I A etc. in the red sage root water extraction dregs of a decoction.Its content is respectively at 0.7mgg
-1, 1.3mgg
-1, 3.4mgg
-1, 1.7mgg
-1above.These materials have stronger anti-oxidant, anti-hepatic fibrosis, anti-inflammation, neuroprotective cell, cell death inducing and the pharmacological action such as antitumor, and treating scytitis, coronary heart diseases and angina pectoris etc. is clinically widely used; Except these liposoluble active compositions, also containing a large amount of celluloses in the red sage root dregs of a decoction, lignin and a small amount of protein, starch etc. can utilize nutrient component.Therefore, the red sage root dregs of a decoction are also a kind of renewable resourcess with higher application prospect.At present, the red sage root dregs of a decoction generally transport plant area by production unit, take stacking, landfill, burning disposal, wherein stack for principal mode.Owing to being generally deposited in field, mountain area, outlying area place, because of rainwater shower, cause stacking place ambient contamination more, especially even more serious on the impact of water quality.These traditional methods of disposal not only cost high, easily bring pollution, and also result in a large amount of wastings of resources.If can realize recycling to it, fair margin of profit will be brought for enterprise.Meanwhile, the exploitation red sage root dregs of a decoction realize the recycling of resource, effectively can prevent resource loss, avoid environmental pollution, meet current national Developing Circulation Economy, the poolicy orientation of building a conservation-minded society.
Grifola frondosus is a kind of medicine, food dual-purpose gill fungus bacterium.Fruit body endoplasm, handle is short in coralliform branch, and meat is tender and crisp tasty and refreshing; There is high health-care effect and medical value.According to reported in literature, it has the effect suppressing hypertension and obesity; Owing to being rich in iron, copper and Vitamin C, it can prevent anaemia, scurvy, leucoderma, prevents the generation of arteriosclerosis and cerebral thrombus; Its selenium and chromium content higher, have protection liver, pancreas, prevention cirrhosis and the effect of diabetes; Se content is high to be made it also have to prevent and treat Keshan disease, Kaschin-Beck disease and some cardiopathic function; Its double calcic and vitamin D, both coordinate, and effectively can prevent and treat rickets; The favourable brain development of higher Zn content, maintenance oxyopia, promote wound healing; The vitamin E of high-load and selenium coordinate, and make it anti-ageing, strengthen memory and sensitivity.Grifola frondosus or noticeable anticarcinogen source, on the one hand, higher Se content has the effect resisting cancer, especially contained grifolan, based on beta glucan, wherein active anticancer is the strongest, the β-(1 of band 6 side chains, 3)-glucan accounts for sizable proportion, and its effect has stronger anti-cancer ability than the lentinan appeared on the market, coriolan etc.; Be again fabulous immunomodulator, therefore, grifola frondosus is a kind of very valuable medicine-food two-purpose fungi simultaneously.But artificial cultivation biological transformation ratio is lower at present, still aobvious rare on market at home, price is higher.
The object of the invention is to utilize the red sage root water extraction dregs of a decoction for Main Cultivation bag raw material, cultivate and produce medicine-food two-purpose fungi grifola frondosus and the technology adapted with it is provided.Achieve the recycling of the red sage root dregs of a decoction, avoid the environmental pollution brought in conventional process, turn waste into wealth.For the recycling of the red sage root dregs of a decoction opens up a new way.
Summary of the invention
The object of the invention is the problem of waste and the environmental pollution existed for the process of this reproducible utilization resource of the current red sage root dregs of a decoction, the method utilizing the red sage root dregs of a decoction to prepare the planting material of grifola frondosus for primary raw material is provided, the method effectively can not only fully utilize the discarded red sage root dregs of a decoction, reduce environmental pollution, and adopt the liquid spawn of red sage root dregs of a decoction domestication, decreasing pollution shortens the production of hybrid seeds cycle simultaneously, grifola frondosus is in the cultivation of major ingredient at the red sage root dregs of a decoction, mycelial growth is evenly vigorous, cultivation cycle is short, fruiting early, change of tide is fast, the grifola frondosus nutritive value produced reach with trees, the grifola frondosus that cotton seed hulls etc. obtain as main material production is equal to.Containing a large amount of mycoprotein, active polysaccharide in cultivation foodstuff after grifola frondosus of gathering.Greatly reduce compared with the foodstuff that crude fiber content and traditional cotton seed hulls, straw, weed tree sawdust etc. are primary raw material, Production of Livestock and Poultry can be used for as feed addictive and also can be used as the cultivation of organic manure for famous and precious plant.
Concrete scheme of the present invention is as follows:
Grifola frondosus of the present invention (Grifola frondosa5.00694) liquid spawn makes and the Cultivation use red sage root dregs of a decoction are the residue that after hot water extraction, remaining fresh nothing is gone mouldy, wherein moisture 75-85%, full nitrogen (butt) is 2.5-3.5%, and Thick many candies (butt) is 2.1-3.5%.
First grifola frondosus of the present invention (Grifola frondosa5.00694) liquid spawn carries out 25 DEG C and cultivates 7-10d on test tube slant before making inoculation, cultivate the slant medium of use for PDA medium is (containing sucrose 2%, agar 2%), then from cultivating ripe inclined-plane with in lower two ferfas blocks access level liquid seed culture medium (300mL triangular flask loading amount 40mL) of inoculation shovel shovel, about 0.5cm × 0.5cm size, liquid culture based formulas is the fresh salvia miltiorrhiza dregs of a decoction 20%, corn flour 1%, glucose 1%, peptone 0.5%, magnesium sulfate 0.02%, initial pH6.0-7.0.Postvaccinal liquid culture shaking flask is placed on 25-28 DEG C, in the constant temperature oscillator of 160r/min, cultivates 5-6d.After the domestication of 1-2d in red sage root dregs of a decoction level liquid seed culture medium is cultivated, the quantity of the bacterium ball in medium increases gradually, and bacterium ball is tiny grain of rice shape, and 5-6d rear stage liquid seed culture medium becomes clarification gradually, thickness, level liquid seed is cultivates maturation.Again by 10% access secondary liquid seed culture medium of inoculum concentration, secondary seed medium formula is the fresh salvia miltiorrhiza dregs of a decoction 30%, corn flour 1%, glucose 1%, rice straw powder 0.5%, magnesium sulfate 0.02%, pH6.0-7.0.Initial pH is 6-7, and postvaccinal liquid culture shaking flask is placed on 25-28 DEG C, cultivates 4d and disappear to the fibrous material in medium in the constant temperature oscillator of 160r/min, only the surplus bacterium ball liquid having thickness.
Of the present invention with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivation is get to cultivate ripe liquid grifola frondosus (Grifola frondosa5.00694) seed and access sterilizing and be cooled in the solid state cultivation bag of about 30 DEG C in superclean bench, in solid state cultivation bag, culture medium prescription is fresh salvia miltiorrhiza dregs of a decoction 80%-85%, wheat bran 15%-19%, glucose 1%, gypsum 1%, initial pH controls as 7.0-7.5, inoculum concentration controls 15%, i.e. 100 grams of solid culture medium access 15ml liquid spawns.Postvaccinal cultivation bag is placed on 25-28 DEG C, cultivate in the dark room of humidity about 70%, treat that mycelia is covered with, mycelium differentiation kink forms fruit body primordium, the projection of adularescent or pitchy can be seen, now want fully printing opacity ventilation at once, create day and night temperature, keep relative air humidity 90-95%.Can spray thin water in media surface, keep charge level not ponding, along with the overstriking of growing tall of stem, cap is grown up, and now can spray 3-4 water every day, until gather.
Containing more protein and abundant active polysaccharide in cultivation foodstuff after fruiting is gathered of the present invention, wherein crude protein content is about 30% (in butt), true protein content accounts for more than 90% of crude protein content, greatly reduce compared with the foodstuff that crude fiber content and traditional cotton seed hulls, straw, weed tree sawdust etc. are primary raw material, Production of Livestock and Poultry can be used for as feed addictive and also can be used as the cultivation of organic manure for famous and precious plant.
Embodiment
Embodiment 1
1, grifola frondosus (Grifola frondosa5.00694) liquid seeds preparation
(1) level liquid seed culture based formulas: the fresh salvia miltiorrhiza dregs of a decoction 20%, corn flour 1%, glucose 1%, peptone 0.5%, magnesium sulfate 0.02%, pH6.0-7.0;
(2) secondary liquid seed culture based formulas: the fresh salvia miltiorrhiza dregs of a decoction 30%, corn flour 1%, glucose 1%, rice straw powder 0.5%, magnesium sulfate 0.02%, pH6.0-7.0.
(3) sterilizing methods charging latter 121 DEG C, be cooled to after 0.1MP sterilizing sterilizing in 30 minutes 30 DEG C for subsequent use.
(4) (every block is as the criterion to inoculate shovel size inoculation method level liquid seed access grifola frondosus (Grifola frondosa5.00694) slant strains 2-3 block, be generally 0.5cm × 0.5cm size), operate on aseptic operating platform and carry out.Secondary liquid seed has cultivated ripe level liquid seed by the inoculum concentration inoculation of 15%
(5) postvaccinal liquid culture shaking flask is placed on 25-28 DEG C by cultivation, cultivates 5-6d in the constant temperature oscillator of 160r/min.After the domestication of 1-2d in red sage root dregs of a decoction level liquid seed culture medium is cultivated, the quantity of the bacterium ball in medium increases gradually, bacterium ball is tiny grain of rice shape, and 5-6d rear stage liquid seed culture medium becomes clarification, thickness gradually, and now level liquid seed is and cultivates maturation.Again by 10% access secondary liquid seed culture medium of inoculum concentration, postvaccinal liquid culture shaking flask is placed on 25-28 DEG C, cultivates 4d in the constant temperature oscillator of 160r/min and disappear to the fibrous material in medium, only the surplus bacterium ball liquid having thickness.
2, with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivation
(1) culture material formula fresh salvia miltiorrhiza dregs of a decoction 80%-85%, wheat bran 15%-19%, glucose 1%, gypsum 1%, initial pH controls as 7.0-7.5
(2) spice takes composts or fertilisers of cultivating according to the above ratio, is adjusted to suitable water content.Notice that moisture can not once add, add in batches, and mix evenly.
(3) adjust pH makes pH value at 7.0-7.5.Meta-alkali, regulates with citric acid; Meta-acid, regulates with pulverized limestone.Add in the mode of spraying, spray limit, limit turns.
(4), after charging, tying material are mixed, rapid packing.With the polypropylene plastics wound packages material of 23cm × 55cm, limit rim compacting, treats that composts or fertilisers of cultivating installs, with the flat charge level of hand, then with perforation rod in material central punch.The two ends of bag can add tampon sealing with neck ring or tie with rope reflexed.Pocket requires that two is loose in the middle of tight, along the tight inwall pine of bag wall.
(5) after sterilizing material installs, should sterilizing in time, prevent matrix acid from becoming.High pressure steam sterilization, sterilizing 1-2h, temperature is about 121 DEG C.
(6), after cooling sterilizing terminates, take out pocket and be placed in transfer room.Be cooled to about 30 DEG C.
(7) 100 milliliters of syringes are got in inoculation, remove syringe needle and change an internal diameter 1-2 millimeter, a strain inoculation device made by the stainless steel of long 100-200 millimeter or aluminum pipe, clean inoculator before using and bind up with gauze dress plastic sack, cool after high-temperature sterilization, extracting liquid bacterial classification can be inoculated, bag or bottleneck inoculation can be opened, also directly syringe needle can be inserted (first with 75% alcohol or iodine disinfection import department) injection inoculation in seed bottle (bag) sealing film, adhesive plaster sealing can be pasted after inoculation, also can dry lime sealing.Inoculum concentration is 15%.
(8) send out bacterium cultivation and postvaccinal culture bag is placed on 25-28 DEG C, cultivate in the dark room of humidity about 70%, treat that mycelia is covered with, mycelium differentiation kink forms fruit body primordium, the projection of adularescent or pitchy can be seen, now want fully printing opacity ventilation at once, create day and night temperature, keep relative air humidity 90-95%.Can spray thin water in media surface, keep charge level not ponding, along with the overstriking of growing tall of stem, cap is grown up, and now can spray 3-4 water every day, until gather.
Cultivate and management parameters:
A, temperature: grifola frondosus (Grifola frondosa5.00694) mycelial growth optimum temperature is 25 DEG C-28 DEG C, and fruit-body formation optimum temperature is 20 DEG C-22 DEG C.
B, humidity: vegetative stage air humidity is 70% to be advisable, and based on dark, strong mycelial growth.
If mycelia does not sprout in C local, the ventilation of several hole played by available aseptic inoculation shovel, also can spray the alcohol mixeding liquid of 10% sugar and 20%, impel mycelium germination.
(9) after cultivating foodstuff drying, can directly as feed addictive or as organic manure.
Claims (6)
1. one kind with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivates production technology, be primarily characterized in that with this reproducible utilization resource of the red sage root dregs of a decoction as primary raw material culturing edible fungus grifola frondosus (Grifola frondosa5.00694), effectively can fully utilize the discarded red sage root dregs of a decoction, reduce environmental pollution, adopt the liquid spawn of red sage root dregs of a decoction domestication simultaneously, shorten the production of hybrid seeds cycle, grifola frondosus (Grifola frondosa5.00694) is in the cultivation of major ingredient at the red sage root dregs of a decoction, mycelial growth is evenly vigorous, the grifola frondosus nutritive value produced reach with trees, cotton seed hullss etc. are produced as major ingredient the grifola frondosus obtained and are equal to.Cultivate foodstuff and contain abundant true albumen, active polysaccharide etc., raw fiber content compared with traditional foodstuff greatly reduces, and the cultivation waste material after grifola frondosus of gathering can directly use as feed addictive or organic manure.
2. one according to claim 1 with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivates production technology, it is characterized in that described grifola frondosus (Grifola frondosa5.00694) liquid spawn makes level liquid seed culture medium is the fresh salvia miltiorrhiza dregs of a decoction 20%, corn flour 1%, glucose 1%, peptone 0.5%, magnesium sulfate 0.02%, pH6.0-7.0; Secondary liquid seed culture medium is the fresh salvia miltiorrhiza dregs of a decoction 30%, corn flour 1%, glucose 1%, rice straw powder 0.5%, magnesium sulfate 0.02%, pH6.0-7.0.
3. according to claim 1 and according to claim 2 with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivates production technology, it is characterized in that grifola frondosus (Grifola frondosa5.00694) liquid spawn makes, grifola frondosus (Grifola frondosa5.00694) slant strains tames 1-2d in red sage root dregs of a decoction level liquid seed culture medium, cultivation temperature 25-28 DEG C, shaking speed 160r/min, continues to be cultured to 5-6d rear stage liquid seeds and cultivates ripe.Secondary liquid seed culture, inoculum concentration 10%, cultivation temperature 25-28 DEG C, shaking speed 160r/min, cultivate 4d and disappear to the fibrous material in medium, only the surplus bacterium ball liquid having thickness, and liquid spawn is ripe.
4. one according to claim 1 with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivates production technology, plant formulation is fresh salvia miltiorrhiza dregs of a decoction 80%-85%, wheat bran 15%-19%, glucose 1%, gypsum 1%, pH7-7.5.
5. the one according to claim 1,2,3 and 4 with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivates production technology, be primarily characterized in that grifola frondosus (Grifola frondosa5.00694) cultivation is cultivated and management parameters is (1) temperature: mycelial growth optimum temperature is 25 DEG C-28 DEG C, and fruit-body formation optimum temperature is 20 DEG C-22 DEG C.(2) humidity: vegetative stage air humidity is 70% to be advisable, and based on dark, strong mycelial growth.(3) ventilate 1-2 time every day, if local mycelia does not sprout, the ventilation of several hole played by available aseptic inoculation shovel, also can spray the alcohol mixeding liquid of 10% sugar and 20%, impel mycelium germination.
6. according to claim 1, 2, 3, one described in 4 and 5 with the red sage root dregs of a decoction for cultivation bag major ingredient grifola frondosus (Grifola frondosa5.00694) cultivates production technology, be primarily characterized in that in the cultivation waste material after fruiting is gathered, crude protein content is about 12% (in butt), true protein content accounts for more than 80% of crude protein content, soluble polysaccharide content is more than 15%, crude fiber content and traditional cotton seed hulls, straw, the foodstuff that weed tree sawdust etc. are primary raw material is compared and is greatly reduced, Production of Livestock and Poultry can be used for as feed addictive and also can be used as the cultivation of organic manure for famous and precious plant.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106631247A (en) * | 2016-11-16 | 2017-05-10 | 株洲千金药业股份有限公司 | Gynecological thousand golden prescription decoction dreg organic fertilizer for pinellia ternate cultivation and preparation method thereof |
| CN108299045A (en) * | 2017-09-14 | 2018-07-20 | 西北师范大学 | A kind of culture medium for cultivating schizophyllum commune |
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| CN100432212C (en) * | 2006-05-15 | 2008-11-12 | 徐泽平 | Grifola frondosa strain, culture method and application thereof |
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Application publication date: 20151104 |