CN105016909A - Oyster mushroom culture medium utilizing Pleurotus eryngii waste and method for cultivating oyster mushrooms with culture medium - Google Patents

Oyster mushroom culture medium utilizing Pleurotus eryngii waste and method for cultivating oyster mushrooms with culture medium Download PDF

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Publication number
CN105016909A
CN105016909A CN201510515769.1A CN201510515769A CN105016909A CN 105016909 A CN105016909 A CN 105016909A CN 201510515769 A CN201510515769 A CN 201510515769A CN 105016909 A CN105016909 A CN 105016909A
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Prior art keywords
pleurotus eryngii
culture medium
eryngii waste
waste material
mushroom
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CN201510515769.1A
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Inventor
陈翠翠
巩玉辉
宋芳芳
何洪胜
司昌雷
安志勇
隋海周
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QINGDAO HSLN AGRICULTURAL TECHNOLOGY Co Ltd
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QINGDAO HSLN AGRICULTURAL TECHNOLOGY Co Ltd
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Abstract

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to an oyster mushroom culture medium utilizing Pleurotus eryngii waste and a method for cultivating oyster mushrooms with the culture medium. The oyster mushroom culture medium utilizing the Pleurotus eryngii waste comprises raw materials in percentage by mass as follows: 20%-25% of cotton seed hulls, 0%-30% of corn cobs, 30%-60% of the Pleurotus eryngii waste, 10%-15% of wheat bran, 1%-2% of gypsum and 1%-3% of lime. According to the oyster mushroom culture medium utilizing the Pleurotus eryngii waste and the method for cultivating the oyster mushrooms with the culture medium, the oyster mushrooms are cultivated with the Pleurotus eryngii waste, the imbalance between supply and demand of the cotton seed hulls is eliminated, the production cost is reduced, most importantly, the use value of the Pleurotus eryngii waste is increased, the problem of environmental pollution caused by the Pleurotus eryngii waste is solved, the Chinese recycling ecological agriculture concept is met, and a new way is provided for sustainable development of Pleurotus eryngii.

Description

A kind ofly utilize the oyster mushroom culture medium of Pleurotus eryngii waste material and the method with its cultivating white mushroom
Technical field
The invention belongs to fungus growing technique field.More specifically, the present invention relates to and a kind ofly utilize the oyster mushroom culture medium of Pleurotus eryngii waste material and the method with its cultivating white mushroom.
Background technology
Flat mushroom, because of its delicious flavour, nutritious and have good food therapy effect, can improve human metabolism, build up health, the effect such as regulating plant neural function, is loved by the people.Along with the development of China's mushroom industry, raw material such as the prices such as cotton seed hulls, corn cob, wheat bran, rice bran in order to culturing edible fungus all improve constantly, to cotton seed hulls in 2015 to reach 2000 yuan/ton.And the price of edible fungus lags far behind the rise of cost of material, the benefit of peasant's culturing edible fungus reduces greatly.In addition, planting almond abalone mushroom process is only 40-45 days, only goes out a damp mushroom, and also have nutritive substance not greatly to be converted, to absorb in Pleurotus eryngii waste material, Pleurotus eryngii mycelia contains a large amount of protein simultaneously, and nutritive substance enriches.If by Pleurotus eryngii waste material directly process can cause environmental pollution, therefore, how to improve the utility value of Pleurotus eryngii waste material, solve the problem of environmental pollution that it causes, Sustainable development for Pleurotus eryngii improves a new approach, becomes the problem that edible mushrooms industry needs research and development.
Summary of the invention
The present invention is intended to utilize Pleurotus eryngii waste material to replace part cotton seed hulls to prepare oyster mushroom culture medium, and a kind of method of its cultivating white mushroom is provided, realize the recycling capable of circulation of organic waste materials, reduce production cost, increase yield, improve the utility value of Pleurotus eryngii waste material, the problem of environmental pollution that the imbalance between supply and demand of solution cotton seed hulls and Pleurotus eryngii waste material cause, to meet the theory of China's ecological agriculture capable of circulation.
The present invention is achieved through the following technical solutions.
Utilize an oyster mushroom culture medium for Pleurotus eryngii waste material, it is made up of the raw material of following mass percent: cotton seed hulls 20-25%, corn cob 0-30%, Pleurotus eryngii waste material 30-60%, wheat bran 10-15%, gypsum 1-2%, lime 1-3%.
By a method for described substratum cultivating white mushroom, it comprises the steps:
(1) Pleurotus eryngii waste material is pulverized in advance, dry for subsequent use;
(2) take the cotton seed hulls of formula ratio, corn cob and the Pleurotus eryngii waste material after pre-treatment to prewet;
(3) add the wheat bran of formula ratio, lime and gypsum, add suitable quantity of water after stirring in compound to control moisture at 60-65%, continue to stir;
(4) pack, seal;
(5) sterilising treatment is carried out;
(6), after end subject to sterilization, it is cooled;
(7) inoculate when temperature is down to room temperature;
(8) inoculate the complete culturing room that puts into cultivate, bacterium pending is terminated, and bacterium bag is moved into fruiting booth and carries out management of producing mushroom, can gather when flat mushroom grows to 7-8 maturation.
The bacterium bag that described Pleurotus eryngii waste material adopts the same day, fresh nothing is gone mouldy or bacterium bottle.
In described step (2), in advance 24-48h carries out water pouring, turning prewets, and the time of prewetting is 24h, keeps prewetting moisture 65%.
First stir with one-level stirrer after adding suitable quantity of water in described step (3), then enter secondary blender continuation stirring.
Send into spiral sack packer by travelling belt in described step (4) to pack, edible fungus bag adopts polyethylene or polypropylene plastics pocket, when it is polyethylene plastic bag, in described step (5), sterilizing parameter is: atmospheric steam sterilizing, sterilising temp is 100-105 DEG C, sterilization time is 10-12h, and the stewing time of putting is 6-8h; When it is polypropylene plastics pocket, in described step (5), sterilizing parameter is: high pressure steam sterilization, and sterilising temp is 121 DEG C-126 DEG C, and sterilization time is 2-4h, and the stewing time of putting is 6-8h.
Inoculation adopts one end to transplant the inoculation method of bar in described step (7), and carries out the inoculating process of front cover with flat mushroom solid spawn, and vaccination ways also can adopt conventional flat mushroom inoculating process;
In described step (8), culturing room's temperature controls at 20-26 DEG C, and humidity is 60-70%, and incubation time is 20-30 days, till mycelia covers with bacterium bag, the humidity of flat mushroom management of producing mushroom remains on 85-90%, and gas concentration lwevel controls at below 3000ppm, and illumination is 200-500lux.
The beneficial effect that the present invention compared with prior art has is:
(1) cultivating method of the present invention often can wrap and save flat mushroom production cost 0.8-1.2 unit, and the simple advantage that also can be applicable to scale operation of this preparation method.
(2) the present invention utilizes Pleurotus eryngii waste material cultivating white mushroom, not only solve the imbalance between supply and demand of cotton seed hulls, reduce production cost, most importantly improve the utility value of Pleurotus eryngii waste material, solve the problem of environmental pollution that Pleurotus eryngii waste material causes, meet the theory of China's ecological agriculture capable of circulation, the Sustainable development for Pleurotus eryngii improves a new approach.
Embodiment
Embodiment 1
Implement place: bacterium job contract factory of Qingdao Hua Shenglvneng agricultural science and technology company limited and 508 farm A2 photovoltaic facility plastic greenhouses.
Utilize an oyster mushroom culture medium for Pleurotus eryngii waste material, it comprises the component of following weight:
Cotton seed hulls 25%, Pleurotus eryngii waste material 60%, wheat bran 13%, gypsum 1%, lime 1%.
By a method for described substratum cultivating white mushroom, it comprises the following steps:
(1) by formula in Pleurotus eryngii waste material pulverize in advance, dry for subsequent use;
(2) taking the cotton seed hulls of formula ratio, corn cob and the Pleurotus eryngii waste material after pre-treatment adopts water method of drenching to prewet, and the time of prewetting is 24h;
(3) add the wheat bran of formula ratio, lime and gypsum, stir with stirrer, add substratum siccative and weigh the water of 1.2 times in compound, ensure that moisture is at about 60-65%, uses 12m 3one-level stirrer stirs 40min, stirs, and then enters secondary blender and stirs;
(4) send into sack packer by travelling belt, adopt spiral sack packer to pack, edible fungus bag adopts the polyethylene edible fungus bag of 22cm*40cm*5C, and work loading height is 23cm, and sealing sealing machine is shut;
(5) sterilization basket put into by the bacterium bag packed, and puts 5 for every basket, puts into sterilizing dolly, enter normal-pressure sterilizing pot, and sterilization time is 12h, and sterilising temp is 100 DEG C, boils in a covered pot over a slow fire and puts time 6h;
(6), after sterilizing terminates, put into cooling room and cool;
(7) inoculate when bacterium bag is cooled to 25 DEG C, adopt the inoculation method of a bar of transplanting;
(8) inoculate the complete culturing room that puts into cultivate, culturing room temperature 20-25 DEG C, humidity is 60-70%, cultivate about 20 days mycelia and cover with bacterium bag, the bacterium bag sending out full bacterium is moved into 508 farm A2 photovoltaic facility plastic greenhouses and carries out added fruiting, humidity remains on 85-90%, and gas concentration lwevel controls at below 3000ppm, illumination is 200-500lux, treats that flat mushroom grows to 7-8 maturation and can gather.
In the present embodiment, oyster mushroom culture medium cost control is at 0.71 yuan/bag, and total cost controls at 1.2-1.4 unit/bag, and the flat mushroom biological transformation ratio of cultivating can reach 144.5%, and having mycelia, to send out bacterium fast, grow strong, output is high, turn ultrafast feature.
Embodiment 2
Implement place: bacterium job contract factory of Qingdao Hua Shenglvneng agricultural science and technology company limited and 508 farm A2 photovoltaic facility plastic greenhouses.
Utilize an oyster mushroom culture medium for Pleurotus eryngii waste material, it comprises the component of following weight:
Cotton seed hulls 20%, corn cob 35%, Pleurotus eryngii waste material 30%, wheat bran 13%, gypsum 1%, lime 1%.
By a method for described substratum cultivating white mushroom, it comprises the following steps:
(1) by formula in Pleurotus eryngii waste material pulverize in advance, dry for subsequent use;
(2) taking the cotton seed hulls of formula ratio, corn cob and the Pleurotus eryngii waste material after pre-treatment adopts water method of drenching to prewet, and the time of prewetting is 24h;
(3) add the wheat bran of formula ratio, lime and gypsum, stir with stirrer, add substratum siccative and weigh the water of 1.2 times in compound, ensure that moisture is at about 60-65%, uses 12m 3one-level stirrer stirs 40min, stirs, and then enters secondary blender and stirs;
(4) send into sack packer by travelling belt, adopt spiral sack packer to pack, edible fungus bag adopts the polyethylene edible fungus bag of 22cm*40cm*5C, and work loading height is 23cm, and sealing sealing machine is shut;
(5) sterilization basket put into by the bacterium bag packed, and puts 5 for every basket, puts into sterilizing dolly, enter normal-pressure sterilizing pot, and sterilization time is 12h, and sterilising temp is 100 DEG C, boils in a covered pot over a slow fire and puts time 6h;
(6), after sterilizing terminates, put into cooling room and cool;
(7) inoculate when bacterium bag is cooled to 25 DEG C, adopt the inoculation method of a bar of transplanting;
(8) inoculate the complete culturing room that puts into cultivate, culturing room temperature 20-25 DEG C, humidity is 60-70%, cultivate about 20 days mycelia and cover with bacterium bag, the bacterium bag sending out full bacterium is moved into 508 farm A2 photovoltaic facility plastic greenhouses and carries out added fruiting, humidity remains on 85-90%, and gas concentration lwevel controls at below 3000ppm, illumination is 200-500lux, treats that flat mushroom grows to 7-8 maturation and can gather.
In the present embodiment, oyster mushroom culture medium cost control is at 0.77 yuan/bag, and total cost controls at 1.3-1.5 unit/bag, and the flat mushroom biological transformation ratio of cultivating can reach 135.4%, and having mycelia, to send out bacterium fast, grow strong, output is high, turn ultrafast feature.
Embodiment 3
Implement place: bacterium job contract factory of Qingdao Hua Shenglvneng agricultural science and technology company limited and 508 farm A2 photovoltaic facility plastic greenhouses.
Utilize an oyster mushroom culture medium for Pleurotus eryngii waste material, it comprises the component of following weight:
Cotton seed hulls 20%, corn cob 20%, Pleurotus eryngii waste material 45%, wheat bran 13%, gypsum 1%, lime 1%.
The described preparation method utilizing the oyster mushroom culture medium of Pleurotus eryngii waste material, it comprises the following steps:
(1) by formula in Pleurotus eryngii waste material pulverize in advance, dry for subsequent use;
(2) taking the cotton seed hulls of formula ratio, corn cob and the Pleurotus eryngii waste material after pre-treatment adopts water method of drenching to prewet, and the time of prewetting is 24h;
(3) add the wheat bran of formula ratio, lime and gypsum, stir with stirrer, add substratum siccative and weigh the water of 1.2 times in compound, ensure that moisture is at about 60-65%, uses 12m 3one-level stirrer stirs 40min, stirs, and then enters secondary blender and stirs;
(4) send into sack packer by travelling belt, adopt spiral sack packer to pack, edible fungus bag adopts the polyethylene edible fungus bag of 22cm*40cm*5C, and work loading height is 23cm, and sealing sealing machine is shut;
(5) sterilization basket put into by the bacterium bag packed, and puts 5 for every basket, puts into sterilizing dolly, enter normal-pressure sterilizing pot, and sterilization time is 12h, and sterilising temp is 100 DEG C, boils in a covered pot over a slow fire and puts time 6h;
(6), after sterilizing terminates, put into cooling room and cool;
(7) inoculate when bacterium bag is cooled to 25 DEG C, adopt the inoculation method of a bar of transplanting;
(8) inoculate the complete culturing room that puts into cultivate, culturing room temperature 20-25 DEG C, humidity is 60-70%, cultivate about 20 days mycelia and cover with bacterium bag, the bacterium bag sending out full bacterium is moved into 508 farm A2 photovoltaic facility plastic greenhouses and carries out added fruiting, humidity remains on 85-90%, and gas concentration lwevel controls at below 3000ppm, illumination is 200-500lux, treats that flat mushroom grows to 7-8 maturation and can gather.
In the present embodiment, oyster mushroom culture medium cost control is at 0.72 yuan/bag, and total cost controls at 1.23-1.43 unit/bag, and the flat mushroom biological transformation ratio of cultivating can reach 140.6%, and having mycelia, to send out bacterium fast, grow strong, output is high, turn ultrafast feature.

Claims (8)

1. utilize an oyster mushroom culture medium for Pleurotus eryngii waste material, it is characterized in that, be made up of the raw material of following mass percent: cotton seed hulls 20-25%, corn cob 0-30%, Pleurotus eryngii waste material 30-60%, wheat bran 10-15%, gypsum 1-2%, lime 1-3%.
2., by a method for substratum cultivating white mushroom described in claim 1, it is characterized in that, comprise the steps:
(1) Pleurotus eryngii waste material is pulverized in advance, dry for subsequent use;
(2) take the cotton seed hulls of formula ratio, corn cob and the Pleurotus eryngii waste material after pre-treatment to prewet;
(3) add the wheat bran of formula ratio, lime and gypsum, add suitable quantity of water after stirring in compound to control moisture at 60-65%, continue to stir;
(4) pack, seal;
(5) sterilising treatment is carried out;
(6), after end subject to sterilization, it is cooled;
(7) inoculate when temperature is down to room temperature;
(8) inoculate the complete culturing room that puts into cultivate, bacterium pending is terminated, and bacterium bag is moved into fruiting booth and carries out management of producing mushroom, can gather when flat mushroom grows to 7-8 maturation.
3. the method for cultivating white mushroom according to claim 2, is characterized in that, the bacterium bag that described Pleurotus eryngii waste material adopts the same day, fresh nothing is gone mouldy or bacterium bottle.
4. the method for cultivating white mushroom according to claim 2, is characterized in that, in described step (2), 24-48h carries out water pouring in advance, turning prewets, and the time of prewetting is 24h, keeps prewetting moisture 65%.
5. the method for cultivating white mushroom according to claim 2, is characterized in that, first with one-level stirrer stirs after adding suitable quantity of water in described step (3), then enters secondary blender and continue stirring.
6. the method for cultivating white mushroom according to claim 2, it is characterized in that, send into spiral sack packer by travelling belt in described step (4) to pack, edible fungus bag adopts polyethylene or polypropylene plastics pocket, when it is polyethylene plastic bag, in described step (5), sterilizing parameter is: atmospheric steam sterilizing, and sterilising temp is 100-105 DEG C, sterilization time is 10-12h, and the stewing time of putting is 6-8h; When it is polypropylene plastics pocket, in described step (5), sterilizing parameter is: high pressure steam sterilization, and sterilising temp is 121 DEG C-126 DEG C, and sterilization time is 2-4h, and the stewing time of putting is 6-8h.
7. the method for cultivating white mushroom according to claim 2, is characterized in that, in described step (7), inoculation adopts one end to transplant the inoculation method of bar, and carries out the inoculating process of front cover with flat mushroom solid spawn.
8. the method for cultivating white mushroom according to claim 2, it is characterized in that, in described step (8), culturing room's temperature controls at 20-26 DEG C, humidity is 60-70%, incubation time is 20-30 days, and till mycelia covers with bacterium bag, the humidity of flat mushroom management of producing mushroom remains on 85-90%, gas concentration lwevel controls at below 3000ppm, and illumination is 200-500lux.
CN201510515769.1A 2015-08-20 2015-08-20 Oyster mushroom culture medium utilizing Pleurotus eryngii waste and method for cultivating oyster mushrooms with culture medium Pending CN105016909A (en)

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Cited By (6)

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Publication number Priority date Publication date Assignee Title
CN107750813A (en) * 2017-10-25 2018-03-06 广西龙州北部湾现代农业有限公司 A kind of implantation methods of pleurotus eryngii
CN108782013A (en) * 2018-05-25 2018-11-13 江苏淮香食用菌有限公司 A kind of Pleurotus eryngii culture medium and preparation method thereof
CN109699393A (en) * 2019-03-05 2019-05-03 承德兴春和专用设备制造有限公司 The production technology of edible mushroom small mushroom
CN111149623A (en) * 2020-03-06 2020-05-15 绵阳佐伊精耕科技有限公司 Method for producing morchella cultivars in large scale
CN111837806A (en) * 2020-07-14 2020-10-30 驻马店市农业科学院 Culture medium for cultivating oyster mushrooms
CN112931038A (en) * 2021-02-25 2021-06-11 杨健云 Edible mushroom cultivation method

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107750813A (en) * 2017-10-25 2018-03-06 广西龙州北部湾现代农业有限公司 A kind of implantation methods of pleurotus eryngii
CN108782013A (en) * 2018-05-25 2018-11-13 江苏淮香食用菌有限公司 A kind of Pleurotus eryngii culture medium and preparation method thereof
CN109699393A (en) * 2019-03-05 2019-05-03 承德兴春和专用设备制造有限公司 The production technology of edible mushroom small mushroom
CN111149623A (en) * 2020-03-06 2020-05-15 绵阳佐伊精耕科技有限公司 Method for producing morchella cultivars in large scale
CN111837806A (en) * 2020-07-14 2020-10-30 驻马店市农业科学院 Culture medium for cultivating oyster mushrooms
CN112931038A (en) * 2021-02-25 2021-06-11 杨健云 Edible mushroom cultivation method

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