CN111837806A - Culture medium for cultivating oyster mushrooms - Google Patents

Culture medium for cultivating oyster mushrooms Download PDF

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Publication number
CN111837806A
CN111837806A CN202010681182.9A CN202010681182A CN111837806A CN 111837806 A CN111837806 A CN 111837806A CN 202010681182 A CN202010681182 A CN 202010681182A CN 111837806 A CN111837806 A CN 111837806A
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culture medium
nutrition bag
morchella
oyster mushroom
corncobs
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史红鸽
班新河
魏银初
李九英
许巧
王红梅
王梦扬
杨瑞晗
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Zhumadian academy of agricultural sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

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Abstract

The invention relates to a culture medium for cultivating oyster mushrooms. The culture medium comprises the following components in percentage by mass: 20-60% of morchella nutrition bag waste, 10-20% of cottonseed hulls, 17-47% of corncobs, 9-11% of wheat bran, 1-2% of lime and 1-2% of gypsum. According to the invention, the morchella nutrition bag waste, cottonseed hulls, corncobs, wheat bran, lime and gypsum are used as raw materials to prepare the culture medium, so that the contents of protein, amino acid and ash (beneficial inorganic components) of the oyster mushroom can be improved according to the experiments, the quality of oyster mushroom fruiting bodies is improved, and the nutritive value of the oyster mushroom is more prominent. In addition, the use of the morchella nutrition bag waste material also solves the problem of disposal of the morchella nutrition bag waste material, reduces the consumption of the original raw and auxiliary materials for oyster mushroom cultivation, and has good economic and social benefits.

Description

Culture medium for cultivating oyster mushrooms
Technical Field
The invention belongs to the field of oyster mushroom cultivation, and particularly relates to a culture medium for cultivating oyster mushrooms.
Background
The oyster mushroom is the edible mushroom variety with the widest distribution and the largest cultivation amount in China, has delicious meat quality and rich nutrition, and is rich in various amino acids necessary for human bodies. Under the increasingly more exquisite diet trend of health care, the edible mushroom industry has a situation of rapid development. The oyster mushroom becomes a necessary nutritional food on a dining table.
The oyster mushroom cultivation technology is simple and easy to implement, strong in variety adaptability and short in growth cycle, and is deeply favored by broad edible mushroom cultivators. The culture medium for cultivating oyster mushroom mainly comprises cottonseed hull, corncob and wheat bran, and the proportion of the cottonseed hull, the corncob and the wheat bran is respectively about 30%, 55% and 10%. In recent years, with the continuous expansion of the production scale of oyster mushrooms, the demand of raw and auxiliary materials is continuously increased, and the prices of main raw materials such as cottonseed hulls, corncobs and wheat bran are greatly increased. The production cost of cultivators is increased, the cultivation enthusiasm is influenced, and the development of the oyster mushroom cultivation industry is severely restricted, so that the development of a novel substrate has important significance for scientific and sustainable cultivation of oyster mushrooms.
Disclosure of Invention
The invention aims to provide a culture medium for cultivating oyster mushrooms, which reduces the consumption of original raw and auxiliary materials for cultivating oyster mushrooms and improves the nutritive value of the oyster mushrooms.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a culture medium for cultivating oyster mushrooms comprises the following components in percentage by mass: 20-60% of morchella nutrition bag waste, 10-20% of cottonseed hulls, 17-47% of corncobs, 9-11% of wheat bran, 1-2% of lime and 1-2% of gypsum.
The Morchella esculenta nutrition bag provides external nutrition during the cultivation process of Morchella esculenta. After the morchella strains are sown, when 60-80% of conidia are formed generally, placing a nutrition bag; when the wheat grains in the nutrition bag are utilized to be softened, the nutrition bag is picked out. The nutrition bag is used only once, and the average length of cultivated morchella every 667m2About 1000 bags (14cm multiplied by 28cm multiplied by 0.005cm) of exogenous nutrition bags are placed, the number of the nutrition bags used for cultivating the morchella every year is huge, and the generated nutrition bag waste is also increased rapidly.
According to the invention, the morchella nutrition bag waste, cottonseed hulls, corncobs, wheat bran, lime and gypsum are used as raw materials to prepare the culture medium, so that the contents of protein, amino acid and ash (beneficial inorganic components) of the oyster mushroom can be improved according to the experiments, the quality of oyster mushroom fruiting bodies is improved, and the nutritive value of the oyster mushroom is more prominent. In addition, the use of the morchella nutrition bag waste material also solves the problem of disposal of the morchella nutrition bag waste material, reduces the consumption of the original raw and auxiliary materials for oyster mushroom cultivation, and has good economic and social benefits.
In order to further optimize the growth and impurity resistance of the oyster mushroom, preferably, the culture medium consists of the following components in percentage by mass: 20-40% of morchella nutrition bag waste, 15-20% of cottonseed hulls, 32-47% of corncobs, 9-11% of wheat bran, 1-2% of lime and 1-2% of gypsum. In order to better balance the production cost and the biological efficiency of oyster mushroom cultivation, preferably, the culture medium consists of the following components in percentage by mass: 30-40% of morchella nutrition bag waste, 15-18% of cottonseed hulls, 32-40% of corncobs, 9-11% of wheat bran, 1-2% of lime and 1-2% of gypsum. More preferably, the culture medium for cultivating oyster mushrooms comprises the following components in percentage by mass: 40% of morchella nutrition bag waste, 15% of cottonseed hulls, 32% of corncobs, 10% of wheat bran, 2% of lime and 1% of gypsum.
Preferably, the base material of the morchella nutrition bag waste mainly comprises wheat grains, sawdust, corncobs, cotton hulls, bran and soil according to a weight ratio of 30: 20: 20: 10: 14: 4. the base material of the morchella nutrition bag waste material is the initial raw material of the morchella nutrition bag. The typical formula is as follows: 30% of wheat grains, 20% of sawdust, 20% of corncobs, 10% of cotton hulls, 14% of bran, 1% of gypsum powder, 1% of lime and 4% of soil.
The preparation method of the culture medium for cultivating the oyster mushrooms comprises the following steps: weighing the nutrition bag waste, the cottonseed hulls, the corncobs, the wheat bran, the lime and the gypsum according to the proportion, adding water for pre-wetting, and uniformly stirring.
Preferably, when water is added, the ratio of material to water is 1: 1.5. The prewetting time was 6 h. Under the condition, the water content of the culture material is about 65 percent, and the culture material is suitable for growth and development of oyster mushrooms. The humidity of the culture material is preferably that the culture material is tightly held by hands, and water drops are seen in finger joints without dripping.
Detailed Description
The following describes the practice of the present invention in detail with reference to specific examples.
First, a specific example of the culture medium for oyster Mushroom according to the present invention
Example 1
The culture medium for cultivating oyster mushrooms of the embodiment comprises the following components in percentage by mass: 20% of morchella nutrition bag waste, 20% of cottonseed hulls, 47% of corncobs, 10% of wheat bran, 2% of lime and 1% of gypsum.
The preparation method of the culture medium for cultivating oyster mushroom in this example is as follows:
1) selecting pollution-free and mildewed nutrition bag waste, removing the bags, exposing on cement land, and drying in the sun for later use. Selecting dry, pollution-free and mildewed cottonseed hulls, corncobs and bran.
2) Weighing the raw materials according to the formula, adding water according to the ratio of 1:1.5, pre-wetting for 6 hours, uniformly stirring by using a stirrer, wherein the specification of a fungus bag is 22cm multiplied by 45cm multiplied by 0.0035cm, each bag is wetted by 2.5kg, and sterilizing at normal pressure.
Example 2
The culture medium for cultivating oyster mushrooms of the embodiment comprises the following components in percentage by mass: 40% of morchella nutrition bag waste, 15% of cottonseed hulls, 32% of corncobs, 10% of wheat bran, 2% of lime and 1% of gypsum.
Example 3
The culture medium for cultivating oyster mushrooms of the embodiment comprises the following components in percentage by mass: 60% of morchella nutrition bag waste, 10% of cottonseed hulls, 17% of corncobs, 10% of wheat bran, 2% of lime and 1% of gypsum.
Examples 2 and 3 methods for preparing the culture medium for oyster mushroom cultivation refer to example 1.
Second, test example
2.1 test materials
Test strains: resident research No. 1 was deposited in the laboratory with emphasis on edible fungus research in the city of the Statema shop.
The base material formula of the morchella nutrition bag is as follows: 30% of wheat grains, 20% of sawdust, 20% of corncobs, 10% of cotton hulls, 14% of bran, 1% of gypsum powder, 1% of lime, 4% of soil and natural pH. And collecting the morchella nutrition bag waste according to the conventional process.
2.2 description of the formulation
The numbers of the culture materials of the examples 1 to 3 are formula 1, formula 2 and formula 3, the compositions of formula 4, formula 5 and CK are shown in table 1 in detail, and the culture materials are prepared under the same conditions except that the raw material proportions are different.
Table 1 description of the formulations
Figure BDA0002585895750000031
2.3 spawn running and fruiting management
And (3) inoculating the two ends of the culture bag after cooling, carrying out spawn running culture on the inoculated fungus bag at the room temperature of 20-28 ℃, and carrying out wall type bag arrangement on the fungus bag, wherein the relative air humidity is 70-80%. Regularly checking the temperature of the fungus bags, trying to cool and turning over the bags when the temperature of the bags reaches 30 ℃, and regularly checking the pollution condition of the fungus bags. After the hypha grows full, the bag mouth is sleeved with a ring for fruiting. And carrying out unified fruiting management under the condition of the double-arch greenhouse. The temperature is 20-25 ℃, the relative humidity of air is 85-95%, light is scattered below 200lx, and the color of the sporocarp is bright under good ventilation conditions. Collected when the fruiting body is ripe for six minutes and seven minutes. Each treatment was 50 bags, 3 replicates.
Test example 1 oyster Mushroom hypha growth test
The results of the hypha growth test for Pleurotus Ostreatus with different formulations are shown in Table 2.
TABLE 2 Effect of different formulations on the growth of Pleurotus Ostreatus hyphae
Figure BDA0002585895750000032
Figure BDA0002585895750000041
Note: "+ + + + +" indicates the vigorous growth of hyphae; "+ + + +" indicates that the hyphae are more vigorous; "+ +" indicates general hyphal growth.
As can be seen from Table 2, different compost formulas have a large influence on the growth of hyphae. When the content of the cottonseed hulls and the corncobs in the formula is higher, the hyphae grow fast due to rich nutrition of the culture material, and the hyphae are pure white and dense. The growth speed of hypha is in negative correlation with the addition amount of the nutrition bag waste, when the nutrition bag waste is excessively added, the hypha is relatively thin and weak due to insufficient nutrition supply, the hypha grows slowly, and the hypha is slowly decomposed due to the fact that the nutrition bag waste contains macromolecular organic matters such as cellulose and hemicellulose. As can be seen from the table, the amount of morchella esculenta waste added is inversely related to the growth vigor and hypha speed.
Test example 2 Pleurotus ostreatus resistance test
Taking yellow blight as an index for inspecting the disease resistance of oyster mushroom varieties, referring to a method for calculating the susceptibility rate of plants to bacterial diseases, and taking 90 fungus bags in a test group as a calculation sample. The infection of each 1 fungus bag fruiting body is 1, the incidence rate is 1% -5% high resistance, the incidence rate is 5% -10% high resistance, the incidence rate is more than 10% and less than 20% high resistance, and the incidence rate is more than 20% high resistance. The infection of green mould, trichoderma, walnut meat shaped bacterium and neurospora in the fungus growing period is closely related to the management mode, the cultivation mode and the environmental factors. The results of statistics on the anti-contamination performance of resident No. 1 in the fungus growing period are shown in Table 3.
TABLE 3 results of different formulations on the disease and impurity resistance of Pleurotus Ostreatus
Figure BDA0002585895750000042
As can be seen from the table, with the increase of the morchella waste, the contamination rate of the test strains in the spawn running period and the fruiting period is increased along with the increase of the morchella waste, and the contamination rate is in positive correlation with the contamination rate. In the test formula, the highest bacteria-contaminated rate of the fruiting period can be 15.5%, the highest bacteria-contaminated rate of the fruiting period can be 20.5%, the average bacteria-contaminated rate of the fruiting period is 6.9%, the average bacteria-contaminated rate of the fruiting period is 9.4%, and the bacteria-contaminated rate is high. The reason is that the morchella esculenta waste contains a large amount of organic matters, and various microorganisms are easy to breed in the stacking process, so that the waste has large base quantity and various types of mixed bacteria. Even if sterilized at high temperature, some mixed bacteria with higher temperature resistance exist.
As can be seen from the table, the formulas 4 and 5 have different degrees of yellow blight in the fruiting period, because when morchella esculenta waste materials are added excessively in the formulas, the nutrition of culture materials is insufficient, and excessive cellulose and hemicellulose substances exist at the same time, so that hyphae in the fruiting period are sparse and delicate, the overall resistance of the hyphae is weakened, the pollution rate in the fruiting period is increased, and the yellow blight is easily infected.
Test example 3 biological efficiency test of oyster Mushroom
The biological efficiency was calculated from the total fruiting weight of the first 3 rounds of the statistical test, and the results are shown in table 4.
TABLE 4 Effect of different formulations on the biological efficiency of Pleurotus Ostreatus
Figure BDA0002585895750000051
As can be seen from the table, the addition amount of the morchella nutrition bag waste and the biological efficiency of the test strain are in a negative correlation, and the moisture transfer time of the test strain is increased along with the addition of the morchella waste and is in a positive correlation. The fruiting tide of the test strains is reduced along with the addition of the morchella nutrition bag waste, and the biological efficiency is finally reduced. The biological efficiency of the formula 1 is the highest and reaches 75.5 percent, and the biological efficiency of the formula 2 is 74.2 percent, the biological efficiency of the formula 5 is only 38.9 percent at the lowest, and the difference between the highest biological efficiency and the lowest biological efficiency is 36.6 percent, so that the difference is obvious.
The toadstool nutrition bag waste is added into the oyster mushroom cultivation material, and the biological efficiency of the test strains is greatly influenced. The biological efficiency of the test strains is gradually reduced along with the increase of the proportion of the morchella nutrition bag waste in the cultivation raw materials. According to analysis, the morchella nutrition bag waste contains substances such as lignin and cellulose, a part of nutrition is absorbed by morchella mycelium, other nutrition components are low, and after 3 times of mushrooms are harvested, the phenomenon of insufficient nutrition occurs, so that the later-stage fruiting yield has a certain difference with the contrast.
Test example 5 comparison of nutritional value
Taking the formula of the example 2 as an example, a comparative experiment with a CK group is carried out to examine the influence of the formula of the example on the nutritive value of the oyster mushroom. Oven drying fruiting body, and measuring the content of nutrient components. Amino acid content was determined according to GB 5009.124-2016. Protein content was determined according to GB 5009.5-2016. Fat content was determined according to GB 5009.6-2016. Ash was determined according to GB 5009.4-2016. The results are shown in Table 5.
TABLE 5 nutritional ingredient content (g/100g) of Pleurotus Ostreatus fruiting body with different formulas
Figure BDA0002585895750000061
The oyster mushroom is rich in protein and amino acid, has low fat content, and is an excellent nutritional health food. The oyster mushroom contains not only a large amount of organic substances but also abundant inorganic components which maintain the normal physiological functions of human bodies and have very important functions in the aspect of forming human tissues, and the inorganic substances remained after the dried edible mushroom sporocarp is burnt at high temperature (500-600 ℃) are called as ash.
As can be seen from the table: the test formula has the advantages that the contents of protein, amino acid and ash in the oyster mushroom fruiting bodies are higher than those of a control group, and the content of fat is slightly lower than that of the control group. Namely, the formula of the embodiment is higher in nutritive value than CK group in all aspects, and is a oyster mushroom variety which has higher nutritive value and better meets the requirement of human health.
The following conclusions can be drawn from the above test examples:
(1) with the combination of the test examples 1-4, in the spawn running period, because the morchella nutrition bag waste contains part of granular substances such as wheat grains, corncobs and the like, the oxygen introduction amount in the fungus bag is better, and the growth of hypha is facilitated. The morchella nutrition bag waste can be added into the oyster mushroom cultivation material in a proper amount. However, when the addition amount is large, the contamination rate of the oyster mushroom is easy to increase, and the biological efficiency is reduced.
(2) By combining local conditions, although the biological efficiency of the formula 2 is slightly lower than that of the formula 1, the formula 2 can obviously reduce the use of raw materials for cultivating the cottonseed hulls and the corn cobs, the cultivation cost is obviously lower than that of the formula 1 through comprehensive analysis, and the formula 2 can be selected as an optimal popularization formula. The experimental formula not only can effectively reduce the production cost of cultivators, but also can solve the problem of environmental pollution caused by the morchella nutrition bag waste, and the quality of the oyster mushroom fruiting bodies is improved comprehensively, so that the experimental formula is expected to have good economic and social benefits.

Claims (5)

1. A culture medium for cultivating oyster mushrooms is characterized by comprising the following components in percentage by mass: 20-60% of morchella nutrition bag waste, 10-20% of cottonseed hulls, 17-47% of corncobs, 9-11% of wheat bran, 1-2% of lime and 1-2% of gypsum.
2. The culture medium for cultivating oyster mushroom according to claim 1, comprising the following components by mass percent: 20-40% of morchella nutrition bag waste, 15-20% of cottonseed hulls, 32-47% of corncobs, 9-11% of wheat bran, 1-2% of lime and 1-2% of gypsum.
3. The culture medium for cultivating oyster mushroom according to claim 1, comprising the following components by mass percent: 30-40% of morchella nutrition bag waste, 15-18% of cottonseed hulls, 32-40% of corncobs, 9-11% of wheat bran, 1-2% of lime and 1-2% of gypsum.
4. The culture medium for cultivating oyster mushroom according to claim 3, comprising the following components by mass percent: 40% of morchella nutrition bag waste, 15% of cottonseed hulls, 32% of corncobs, 10% of wheat bran, 2% of lime and 1% of gypsum.
5. The culture medium for cultivating oyster mushroom according to any one of claims 1 to 4, wherein the base material of the morchella nutrition bag waste is mainly composed of wheat grains, wood chips, corncobs, cotton hulls, bran, soil in a weight ratio of 30: 20: 20: 10: 14: 4.
CN202010681182.9A 2020-07-14 2020-07-15 Culture medium for cultivating oyster mushrooms Pending CN111837806A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101302127A (en) * 2008-06-18 2008-11-12 李志明 Special fertilizer for M edible fungus and preparing method thereof
CN103030468A (en) * 2013-01-08 2013-04-10 北京农业生物技术研究中心 Oyster mushroom culture medium and oyster mushroom culture method using same
CN105016909A (en) * 2015-08-20 2015-11-04 青岛华盛绿能农业科技有限公司 Oyster mushroom culture medium utilizing Pleurotus eryngii waste and method for cultivating oyster mushrooms with culture medium
CN105060990A (en) * 2015-08-20 2015-11-18 青岛华盛绿能农业科技有限公司 Oyster mushroom culture medium using mushroom waste and method for cultivating oyster mushrooms through oyster mushroom culture medium
CN105175108A (en) * 2015-08-20 2015-12-23 青岛华盛绿能农业科技有限公司 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus
CN106396807A (en) * 2016-08-31 2017-02-15 天津市宝坤农业科技有限公司 Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101302127A (en) * 2008-06-18 2008-11-12 李志明 Special fertilizer for M edible fungus and preparing method thereof
CN103030468A (en) * 2013-01-08 2013-04-10 北京农业生物技术研究中心 Oyster mushroom culture medium and oyster mushroom culture method using same
CN105016909A (en) * 2015-08-20 2015-11-04 青岛华盛绿能农业科技有限公司 Oyster mushroom culture medium utilizing Pleurotus eryngii waste and method for cultivating oyster mushrooms with culture medium
CN105060990A (en) * 2015-08-20 2015-11-18 青岛华盛绿能农业科技有限公司 Oyster mushroom culture medium using mushroom waste and method for cultivating oyster mushrooms through oyster mushroom culture medium
CN105175108A (en) * 2015-08-20 2015-12-23 青岛华盛绿能农业科技有限公司 Pleurotus ostreatus culture medium and method using same to culture pleurotus ostreatus
CN106396807A (en) * 2016-08-31 2017-02-15 天津市宝坤农业科技有限公司 Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi

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