CN104396564A - Method for substitute cultivation of favolus by utilizing plastic bags - Google Patents

Abstract

The invention discloses a method for the substitute cultivation of favolus by utilizing plastic bags. The method is characterized by including compost preparation, bagging and sterilization, inoculation and cultivation, fungus management, primordium inducement period management, fungus cultivation period management and picking. The culture medium adopted in the steps chooses cotton seed hulls and poplar sawdust as main materials and soybean flour, corn flour, broomcorn flour and calcium carbonate as auxiliary materials, and the plastic bag substitute cultivation method is used for cultivating the favolus. After the favolus is successfully acclimatized and cultivated by the unit, by screening different culture medium formulas and experimentally demonstrating a cultivation pattern, the object of cultivating the acclimatized favolus on a large scale is achieved, and the utilization of the culture medium and the cultivation method can realize the high-quality and high-efficiency cultivation of the favolus, high yield, good quality and high economic benefit.

Description

A kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation

Technical field

Patent of the present invention relates to a kind of fungus growing technique, especially a kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation.

Background technology

Large pore fungi, Polyporaceae macropore Pseudomonas fungi, is mainly distributed in the ground such as Hebei, Henan, Shanxi, Heilungkiang, Liaoning, Tibet, Zhejiang, Anhui, Hunan, Guangxi, Shaanxi, Gansu, Sichuan, Guizhou, Yunnan.

Macropore Pseudomonas in Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, macropore Pseudomonas; Fruit body is without stem or tool short handle, and list is raw or closely grow thickly, bacteria cover diameter 5 ~ 13cm, fan-shaped, kidney-shaped or semicircle, middle part is slightly recessed in shallow funnel-form, smooth surface, shallow khaki, to dirty white, seemingly has ring-type or ring grain, thin edge and slightly involute, bacterial context white, edible during children, meat is tender and crisp, delicious flavour, aging rear approximate cellulosic, tool special bacterium fragrance taste.This bacterium fungi polysaccharide, amino acid content are abundant, and its extract has to sarcoma S-180 and ehrlich carcinoma the effect of obviously drawing up; A kind of rare food, medicine dual-purpose bacterium of having Development volue.

Macropore bacterial classification Yuan Xi our company is wild separating obtained, and entrusted China Committee for Culture Collection of Microorganisms, the preservation of common micro-organisms center, preserving number is CGMCC No.3711, preservation date: on April 6th, 2010, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC).Although wild large pore fungi is tamed, its culture technique does not have maturation, and existing culture technique drawback is too many, and cause large pore fungi to yield poorly, quality is bad, and culture medium cost is high, and the economic benefit of cultivating large pore fungi is not fine.

Summary of the invention

For the problems of the prior art; the object of the invention is to provide a kind of method utilizing the large pore fungi of plastic bag cultivation; large pore fungi after domestication is arranged in plastic sack and cultivates by the method, in conjunction with special nature and the special incubation step of medium, achieves the large-scale planting of large pore fungi; and pass through the combination of this cultural method and composts or fertilisers of cultivating; large pore fungi grows fine, and output is high, and economic benefit is given prominence to; the large pore fungi of bacterium agricultural university large-scale planting can be realized, create larger economic worth.

In order to achieve the above object, technical scheme of the present invention is:

A kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation composts or fertilisers of cultivating: (1) selects materials: choose cotton seed hull, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hull 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, the choosing of major ingredient and auxiliary material be fresh, dry, without the material gone mouldy, (2) prepare: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixed is joined in the major ingredient mixed, again mix, obtained composts or fertilisers of cultivating, finally in compound, add water, stir, composts or fertilisers of cultivating is fully absorbed water, final water content reaches 60% ~ 65%, to notice in the process of this preparation that requirement is accomplished two even one fully (namely material and material evenly, expect with water evenly, absorb water abundant), b, pack sterilizing: select wide 17 ~ 18cm, long 35 ~ 36cm, thickness is at the polyethylene knuckle bag of 0.05 ~ 0.06cm, the often packed composts or fertilisers of cultivating 1250 ~ 1500g mixed, mechanical charging can be adopted, also artificial pack can be adopted, the pocket installed requires degree of tightness appropriateness, about 6cm more than sack, first in the middle of pocket, make a call to the dark hole of a 5cm with loop-carrier, sack film is inserted in hole, the loop-carrier of cap is with to enter pocket from hole interpolation long 16cm again, the upper cap of loop-carrier is compressed and sack of obturaging, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket loads in free flowing steam sterilization pot, sterilizing 8 ~ 12h under normal pressure 100 DEG C ~ 102 DEG C conditions, take the dish out of the pot after vexed 4 ~ 6h again, c, inoculated and cultured: (1) is inoculated: the pocket after being taken the dish out of the pot by step b is transported in clean cooling chamber in time, be cooled to less than 30 DEG C, in inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculation hook, bacterial classification is blended, extract the loop-carrier on pocket, accessed by bacterial classification in excellent hole, the cotton jam-pack after the sterilizing of aperture, bacterial classification will fill up excellent hole, the bottled bacterial classification of general 750ml, can about 25, splicing bag, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environmental temperature height, put 1 ~ 2 layer for every layer, bedstead interlayer will suitably stay a little space, be conducive to air circulation, suitable condition will be created in whole bacteria developing period, to promote mycelia robust growth, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should control at 26 DEG C ~ 27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia immerses vigorous period, because its respiration produces heat, in bag, temperature is often high than room temperature 2 DEG C ~ 4 DEG C, now room temperature is comparatively suitable with 23 DEG C ~ 24 DEG C, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote mycelia robust growth.Through 20 days, bag wall can see mycelia, 30 days, and mycelia can cover with pocket, d, management of producing mushroom: after mycelia covers with bag in step c, continue cultivation 15 ~ 20 days, move in fruiting canopy after making mycelia physiological ripening, code becomes bacterium wall to carry out management of producing mushroom, the management in large pore fungi fruiting stage obtains high-quality, high yield, the key of stable yields: (1) mushroom canopy arranges: before entering bag, first mushroom canopy (room) is interior and mushroom producing multi-layer shelf is cleaned out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up bacterium bag on the spot, in mushroom canopy, laterally or longitudinally wide 25cm ~ 30cm or 50cm ~ 60cm need be put with fragment of brick, the wall base of high 5cm ~ 10 cm, line-spacing 80cm ~ 100cm, use edible mushroom special sterilizing, insecticide, carry out a comprehensively killing, desinsection process, (2) arrange bag, flushing, sterilize, remove plug: by traverse outwards neat for bacterium sack on the wall base, height is advisable with 5 ~ 7 layers, then with high-pressure spray gun, the bacterium bag two ends water set is rinsed well, ventilate 4 ~ 6h, then use sprayer to bacterium bag, booth inwall, ceiling and space atomized spray 1:300 Methylpartricin Sodium Laurylsulfate doubly or the bleaching powder aqueous solution, airtight sterilization 2h ~ 4h, finally removes tampon, the hole of a charge level meeting self-assembling formation diameter 2cm, e, urge flower bud period management: control mushroom temperature of shed at 16 DEG C ~ 22 DEG C, relative air humidity controls 85% ~ 95%, gives the scattered light of 800 lx ~ 1000 lx, adds forced ventilation, keep air fresh, by CO ₂concentration controls below 0.1%, and after 7 days, the former base of warty can be formed in hole, f, educate mushroom period management: after the former base of warty is formed, controlled by relative air humidity 85% ~ 90%, to aerial, wall, the light atomized water spraying in ground, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day, temperature controls at 16 DEG C ~ 20 DEG C, scattered light is at 500 lx ~ 1000 lx, add forced ventilation, gas concentration lwevel controls below 0.1%, g, to gather: large pore fungi buddings and can gather for latter 10 days, when mushroom lid not yet launches by open and flat, spore, is Harvesting Date, and large pore fungi can fruiting 2 ~ 3 tide, the first damp mushroom adds forced ventilation after gathering, stop water spray 4 ~ 5 days, mycelia is rehabilitated, and then recovers management of producing mushroom, the second damp mushroom of can gathering within 3 weeks, require when adopting mushroom that adopting mushroom personnel cuts flat nail, soap clean both hands, dress mushroom cleaning container is clean, and the Methylpartricin Sodium Laurylsulfate aqueous solution sterilization with 0.1% or liquid soap are cleaned, dried.

Further, gathering in step, when specifically gathering, pinching cap base portion with hand, to break down gently or with lancinating down.

Further, if adopt green house to cultivate large pore fungi, can divide spring, autumn two season cultivation, the spring plants can at bag in 2 ~ March, 4 ~ June fruiting; Autumn plants can at bag in August, 9 ~ October fruiting.

The culture technique of large pore fungi is current and immature, this large pore fungi is after my unit domesticating and cultivating success, by the experiment and demonstration of different culture media recipe determination and cultivation mode, explore and utilized cotton seed hull, sawdust for major ingredient, the High-yield Formula that analysis for soybean powder, corn flour, sorghum flour etc. are auxiliary material and utilize the ripe cultivation method of plastic sack, furthermore achieved that the object that the large pore fungi after domestication can be cultivated in enormous quantities, utilize this medium and cultural method, the cultivation of large pore fungi can be made high-quality and efficient, output is high, quality better, economic benefit is superior.

Embodiment

Below in conjunction with embodiment, the present invention is further described.

Embodiment one:

A kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation composts or fertilisers of cultivating: (1) selects materials: choose cotton seed hull, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hull 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, the choosing of major ingredient and auxiliary material be fresh, dry, without the material gone mouldy, (2) prepare: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixed is joined in the major ingredient mixed, again mix, obtained composts or fertilisers of cultivating, finally in compound, add water, stir, composts or fertilisers of cultivating is fully absorbed water, final water content reaches 60% ~ 65%, to notice in the process of this preparation that requirement is accomplished two even one fully (namely material and material evenly, expect with water evenly, absorb water abundant), b, pack sterilizing: select wide 17 ~ 18cm, long 35 ~ 36cm, thickness is at the polyethylene knuckle bag of 0.005 ~ 0.006cm, the often packed composts or fertilisers of cultivating 1250 ~ 1500g mixed, mechanical charging can be adopted, also artificial pack can be adopted, the pocket installed requires degree of tightness appropriateness, about 6cm more than sack, first in the middle of pocket, make a call to the dark hole of a 5cm with loop-carrier, sack film is inserted in hole, the loop-carrier of cap is with to enter pocket from hole interpolation long 16cm again, the upper cap of loop-carrier is compressed and sack of obturaging, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket loads in free flowing steam sterilization pot, sterilizing 8 ~ 12h under normal pressure 100 DEG C ~ 102 DEG C conditions, take the dish out of the pot after vexed 4 ~ 6h again, c, inoculated and cultured: (1) is inoculated: the pocket after being taken the dish out of the pot by step b is transported in clean cooling chamber in time, be cooled to less than 30 DEG C, in inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculation hook, bacterial classification is blended, extract the loop-carrier on pocket, accessed by bacterial classification in excellent hole, the cotton jam-pack after the sterilizing of aperture, bacterial classification will fill up excellent hole, the bottled bacterial classification of general 750ml, can about 25, splicing bag, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environmental temperature height, put 1 ~ 2 layer for every layer, bedstead interlayer will suitably stay a little space, be conducive to air circulation, suitable condition will be created in whole bacteria developing period, to promote mycelia robust growth, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should control at 26 DEG C ~ 27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia immerses vigorous period, because its respiration produces heat, in bag, temperature is often high than room temperature 2 DEG C ~ 4 DEG C, now room temperature is comparatively suitable with 23 DEG C ~ 24 DEG C, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote mycelia robust growth.Through 20 days, bag wall can see mycelia, 30 days, and mycelia can cover with pocket, d, management of producing mushroom: after mycelia covers with bag in step c, continue cultivation 15 ~ 20 days, move in fruiting canopy after making mycelia physiological ripening, code becomes bacterium wall to carry out management of producing mushroom, the management in large pore fungi fruiting stage obtains high-quality, high yield, the key of stable yields: (1) mushroom canopy arranges: before entering bag, first mushroom canopy (room) is interior and mushroom producing multi-layer shelf is cleaned out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up bacterium bag on the spot, in mushroom canopy, laterally or longitudinally wide 25cm ~ 30cm or 50cm ~ 60cm need be put with fragment of brick, the wall base of high 5cm ~ 10 cm, line-spacing 80cm ~ 100cm, use edible mushroom special sterilizing, insecticide, carry out a comprehensively killing, desinsection process, (2) arrange bag, flushing, sterilize, remove plug: by traverse outwards neat for bacterium sack on the wall base, height is advisable with 5 ~ 7 layers, then with high-pressure spray gun, the bacterium bag two ends water set is rinsed well, ventilate 4 ~ 6h, then use sprayer to bacterium bag, booth inwall, ceiling and space atomized spray 1:300 Methylpartricin Sodium Laurylsulfate doubly or the bleaching powder aqueous solution, airtight sterilization 2h ~ 4h, finally removes tampon, the hole of a charge level meeting self-assembling formation diameter 2cm, e, urge flower bud period management: control mushroom temperature of shed at 16 DEG C ~ 22 DEG C, relative air humidity controls 85% ~ 95%, gives the scattered light of 800 lx ~ 1000 lx, adds forced ventilation, keep air fresh, by CO ₂concentration controls below 0.1%, and after 7 days, the former base of warty can be formed in hole, f, educate mushroom period management: after the former base of warty is formed, controlled by relative air humidity 85% ~ 90%, to aerial, wall, the light atomized water spraying in ground, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day, temperature controls at 16 DEG C ~ 20 DEG C, scattered light is at 500 lx ~ 1000 lx, add forced ventilation, gas concentration lwevel controls below 0.1%, g, to gather: large pore fungi buddings and can gather for latter 10 days, when mushroom lid not yet launches by open and flat, spore, is Harvesting Date, and large pore fungi can fruiting 2 ~ 3 tide, the first damp mushroom adds forced ventilation after gathering, stop water spray 4 ~ 5 days, mycelia is rehabilitated, and then recovers management of producing mushroom, the second damp mushroom of can gathering within 3 weeks, require when adopting mushroom that adopting mushroom personnel cuts flat nail, soap clean both hands, dress mushroom cleaning container is clean, and the Methylpartricin Sodium Laurylsulfate aqueous solution sterilization with 0.1% or liquid soap are cleaned, dried.

Embodiment two:

A kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation composts or fertilisers of cultivating: (1) selects materials: choose cotton seed hull, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hull 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, the choosing of major ingredient and auxiliary material be fresh, dry, without the material gone mouldy, (2) prepare: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixed is joined in the major ingredient mixed, again mix, obtained composts or fertilisers of cultivating, finally in compound, add water, stir, composts or fertilisers of cultivating is fully absorbed water, final water content reaches 65%, to notice in the process of this preparation that requirement is accomplished two even one fully (namely material and material evenly, expect with water evenly, absorb water abundant), b, pack sterilizing: select wide 17 ~ 18cm, long 35 ~ 36cm, the thick polyethylene knuckle bag at 0.005 ~ 0.006cm of bag, the often packed composts or fertilisers of cultivating 1500g mixed, mechanical charging can be adopted, also artificial pack can be adopted, the pocket installed requires degree of tightness appropriateness, about 6cm more than sack, first in the middle of pocket, make a call to the dark hole of a 5cm with loop-carrier, sack film is inserted in hole, the loop-carrier of cap is with to enter pocket from hole interpolation long 16cm again, the upper cap of loop-carrier is compressed and sack of obturaging, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket loads in free flowing steam sterilization pot, sterilizing 10h under normal pressure 100 DEG C of conditions, take the dish out of the pot after vexed 45h again, c, inoculated and cultured: (1) is inoculated: the pocket after being taken the dish out of the pot by step b is transported in clean cooling chamber in time, be cooled to less than 30 DEG C, in inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculation hook, bacterial classification is blended, extract the loop-carrier on pocket, accessed by bacterial classification in excellent hole, the cotton jam-pack after the sterilizing of aperture, bacterial classification will fill up excellent hole, the bottled bacterial classification of general 750ml, can about 25, splicing bag, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environmental temperature height, put 1 ~ 2 layer for every layer, bedstead interlayer will suitably stay a little space, be conducive to air circulation, suitable condition will be created in whole bacteria developing period, to promote mycelia robust growth, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should control at 26 DEG C ~ 27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia immerses vigorous period, because its respiration produces heat, in bag, temperature is often high than room temperature 2 DEG C ~ 4 DEG C, now room temperature is comparatively suitable with 23 DEG C ~ 24 DEG C, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote mycelia robust growth.Through 20 days, bag wall can see mycelia, 30 days, and mycelia can cover with pocket, d, management of producing mushroom: after mycelia covers with bag in step c, continue cultivation 18 days, move in fruiting canopy after making mycelia physiological ripening, code becomes bacterium wall to carry out management of producing mushroom, the management in large pore fungi fruiting stage obtains high-quality, high yield, the key of stable yields: (1) mushroom canopy arranges: before entering bag, first mushroom canopy (room) is interior and mushroom producing multi-layer shelf is cleaned out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up bacterium bag on the spot, in mushroom canopy, laterally or longitudinally wide 50cm ~ 60cm need be put with fragment of brick, the wall base of high 5cm ~ 10 cm, line-spacing 80cm ~ 100cm, use edible mushroom special sterilizing, insecticide, carry out a comprehensively killing, desinsection process, (2) arrange bag, flushing, sterilize, remove plug: by traverse outwards neat for bacterium sack on the wall base, height is advisable with 5 ~ 7 layers, then with high-pressure spray gun, the bacterium bag two ends water set is rinsed well, ventilation 5h, then use sprayer to bacterium bag, booth inwall, ceiling and space atomized spray 1:300 Methylpartricin Sodium Laurylsulfate doubly or the bleaching powder aqueous solution, airtight sterilization 3h, finally removes tampon, the hole of a charge level meeting self-assembling formation diameter 2cm, e, urge flower bud period management: control mushroom temperature of shed at 16 DEG C ~ 22 DEG C, relative air humidity controls 85% ~ 95%, gives the scattered light of 800 lx ~ 1000 lx, adds forced ventilation, keep air fresh, by CO ₂concentration controls below 0.1%, and after 7 days, the former base of warty can be formed in hole, f, educate mushroom period management: after the former base of warty is formed, controlled by relative air humidity 85% ~ 90%, to aerial, wall, the light atomized water spraying in ground, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day, temperature controls at 16 DEG C ~ 20 DEG C, scattered light is at 500 lx ~ 1000 lx, add forced ventilation, gas concentration lwevel controls below 0.1%, g, to gather: large pore fungi buddings and can gather for latter 10 days, when mushroom lid not yet launches by open and flat, spore, is Harvesting Date, and large pore fungi can fruiting 2 ~ 3 tide, the first damp mushroom adds forced ventilation after gathering, stop water spray 4 ~ 5 days, mycelia is rehabilitated, and then recovers management of producing mushroom, the second damp mushroom of can gathering within 3 weeks, require when adopting mushroom that adopting mushroom personnel cuts flat nail, soap clean both hands, dress mushroom cleaning container is clean, and the Methylpartricin Sodium Laurylsulfate aqueous solution sterilization with 0.1% or liquid soap are cleaned, dried.

Be understandable that, above about specific descriptions of the present invention, the technical scheme described by the embodiment of the present invention is only not limited to for illustration of the present invention, those of ordinary skill in the art is to be understood that, still can modify to the present invention or equivalent replacement, to reach identical technique effect; Needs are used, all within protection scope of the present invention as long as meet.

Claims (5)

1. one kind utilizes the method for the large pore fungi of plastic sack substituting stuff cultivation, it is characterized in that comprising the steps: a, preparation composts or fertilisers of cultivating: (1) selects materials: choose cotton seed hull, willow sawdust as major ingredient, analysis for soybean powder, corn flour, sorghum flour, calcium carbonate, as auxiliary material, wherein, calculate according to percentage by weight, cotton seed hull 45%, willow sawdust 40%, analysis for soybean powder 6%, corn flour 5%, sorghum flour 3%, calcium carbonate 1%, (2) prepare: first the cotton seed hulls in major ingredient and willow sawdust are mixed, again the analysis for soybean powder in auxiliary material, corn flour, sorghum flour, calcium carbonate are mixed, then the auxiliary material mixed is joined in the major ingredient mixed, again mix, obtained composts or fertilisers of cultivating, finally adds water, stirs in compound, composts or fertilisers of cultivating is fully absorbed water, and final water content reaches 60% ~ 65%, b, pack sterilizing: select wide 17 ~ 18cm, long 35 ~ 36cm, thickness is at the polyethylene knuckle bag of 0.005 ~ 0.006cm, the often packed composts or fertilisers of cultivating 1250 ~ 1500g mixed, the pocket installed requires degree of tightness appropriateness, about 6cm more than sack, first in the middle of pocket, make a call to the dark hole of a 5cm with loop-carrier, sack film is inserted in hole, the loop-carrier of cap is with to enter pocket from hole interpolation long 16cm again, the upper cap of loop-carrier is compressed and sack of obturaging, then sack downwards or be sidelong in iron sterilizing Turnround basket, whole basket loads in free flowing steam sterilization pot, sterilizing 8 ~ 12h under normal pressure 100 DEG C ~ 102 DEG C conditions, take the dish out of the pot after vexed 4 ~ 6h again, c, inoculated and cultured: (1) is inoculated: the pocket after being taken the dish out of the pot by step b is transported in clean cooling chamber in time, be cooled to less than 30 DEG C, in inoculating hood or indoor, first bacterial classification surface aging mycoderma is removed by sterile working, with inoculation hook, bacterial classification is blended, extract the loop-carrier on pocket, bacterial classification is accessed in excellent hole, the cotton jam-pack after the sterilizing of aperture, (2) cultivate: postvaccinal bacterium bag is emitted on multi-stage bedstead or culturing rack, layered putting, depending on environmental temperature height, put 1 ~ 2 layer for every layer, bedstead interlayer will suitably stay a little space, be conducive to air circulation, in postvaccinal 10 days, mycelia is in sprouting setting date, room temperature should control at 26 DEG C ~ 27 DEG C, impel bacterial classification field planting as early as possible, after 10 days, mycelia immerses vigorous period, because its respiration produces heat, in bag, temperature is often high than room temperature 2 DEG C ~ 4 DEG C, now room temperature is comparatively suitable with 23 DEG C ~ 24 DEG C, this one-phase, culturing room's temperature is not lower than 20 DEG C or higher than 27 DEG C, and strengthen room ventilation amount, promote mycelia robust growth,

Through 20 days, bag wall can see mycelia, 30 days, and mycelia can cover with pocket, d, management of producing mushroom: after mycelia covers with bag in step c, continue cultivation 15 ~ 20 days, move in fruiting canopy after making mycelia physiological ripening, code becomes bacterium wall to carry out management of producing mushroom: (1) mushroom canopy arranges: before entering bag, first mushroom canopy (room) is interior and mushroom producing multi-layer shelf is cleaned out, the fresh pulverized limestone of one deck is evenly spread on ground, if pile up bacterium bag on the spot, in mushroom canopy, laterally or longitudinally wide 25cm ~ 30cm or 50cm ~ 60cm need be put with fragment of brick, the wall base of high 5cm ~ 10 cm, line-spacing 80cm ~ 100cm, use edible mushroom special sterilizing, insecticide, carry out a comprehensively killing, desinsection process, (2) arrange bag, flushing, sterilize, remove plug: by traverse outwards neat for bacterium sack on the wall base, height is advisable with 5 ~ 7 layers, then with high-pressure spray gun, the bacterium bag two ends water set is rinsed well, ventilate 4 ~ 6h, then use sprayer to bacterium bag, booth inwall, ceiling and space atomized spray 1:300 Methylpartricin Sodium Laurylsulfate doubly or the bleaching powder aqueous solution, airtight sterilization 2h ~ 4h, finally removes tampon, the hole of a charge level meeting self-assembling formation diameter 2cm, e, urge flower bud period management: control mushroom temperature of shed at 16 DEG C ~ 22 DEG C, relative air humidity controls 85% ~ 95%, gives the scattered light of 800 lx ~ 1000 lx, adds forced ventilation, keep air fresh, by CO ₂concentration controls below 0.1%, and after 7 days, the former base of warty can be formed in hole, f, educate mushroom period management: after the former base of warty is formed, controlled by relative air humidity 85% ~ 90%, to aerial, wall, the light atomized water spraying in ground, cloudy day many by fine day less, the water spray principle regulation and control that stop of rainy day, temperature controls at 16 DEG C ~ 20 DEG C, scattered light is at 500 lx ~ 1000 lx, add forced ventilation, gas concentration lwevel controls below 0.1%, g, to gather: large pore fungi buddings and can gather for latter 10 days, when mushroom lid not yet launches by open and flat, spore, for Harvesting Date, and large pore fungi can fruiting 2 ~ 3 tide, the first damp mushroom adds forced ventilation after gathering, stop water spray 4 ~ 5 days, mycelia is rehabilitated, and then recovers management of producing mushroom, the second damp mushroom of can gathering within 3 weeks.

2. a kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation according to claim 1, is characterized in that: gathering in step, when specifically gathering, pinches cap base portion with hand, breaks down gently or with lancinating down.

3. a kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation according to claim 1, is characterized in that: in step a in composts or fertilisers of cultivating major ingredient and auxiliary material choose be fresh, dry, without the material gone mouldy.

4. a kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation according to claim 1, is characterized in that: during inoculation, and bacterial classification will fill up the excellent hole of loop-carrier.

5. a kind of method utilizing the large pore fungi of plastic sack substituting stuff cultivation according to claim 1, is characterized in that: adopt green house to cultivate large pore fungi, can divide spring, autumn two season cultivation, the spring plants can at bag in 2 ~ March, 4 ~ June fruiting; Autumn plants can at bag in August, 9 ~ October fruiting.