CN109418025A - A kind of cultivating champignon inoculation method - Google Patents
A kind of cultivating champignon inoculation method Download PDFInfo
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- CN109418025A CN109418025A CN201710518398.1A CN201710518398A CN109418025A CN 109418025 A CN109418025 A CN 109418025A CN 201710518398 A CN201710518398 A CN 201710518398A CN 109418025 A CN109418025 A CN 109418025A
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- bacterium bag
- rectangular opening
- inoculation
- rectangle
- mushroom strain
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Abstract
The invention discloses a kind of cultivating champignon inoculation method, genus agaricus technical field of cultivation.It is characterized in that operating as follows: one, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber and sterilizes and is cooled to room temperature;Two, bacterium bag is moved into inoculation indoor inoculation;Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, the mushroom strain that section is rectangle is inserted into the rectangular opening, and sealed with tampon or sponge;The length of the rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.This method keeps strain and the culture medium contact spacing in bacterium bag suitable, and bacterium germination speed is fast, and inoculation efficiency is high.
Description
Technical field
The present invention relates to mushroom-cultivating technical fields.
Background technique
Existing cultivating champignon inoculation method is that culture medium is packed into bacterium bag, swings in sterilizing indoor sterilizing, then again by bacterium
Bag moves into cooling chamber, and when inoculation, which takes, beats cave inocalation method, i.e. bacterium bag is laid out station, got by hand on bacterium bag face by vaccinator
The isometric circle cavities of 3-4 diameter 1.5cm, depth 6cm, with hand by Saw-dust break it is blocking fill in each cavities and compress,
Then bagging or with adhesive tape seal inoculation cave, inoculation complete, then by bacterium bag move into bacteria room carry out Mycelium culture.The method
Drawback is: pollution probability is high;The gap of strain and cultivating in a fungus bag base is big, and the bacterium germination time is long and inoculation efficiency is low.
Summary of the invention
This hair is the technical problem to be solved is that a kind of cultivating champignon inoculation method is provided, with the training in strain and bacterium bag
Support the feature that base gap is suitable, bacterium germination speed is fast, mycelia is non-aging, inoculation efficiency is high.
In order to solve the above technical problems, the technical solution used in the present invention is: a kind of cultivating champignon inoculation method, special
Sign is to operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously
It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening
It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle
Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big
In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The further improvement done to the present invention is:
The edible fungus species of the mushroom strain routine of the rectangle expand numerous technique and are made, and material is broadleaf tree, rule
Lattice: 12cm-18cm, wide 0.6cm-0.8cm, 0.2cm-0.4cm.
The bacterium bag is polyethylene or the dog-ear bag of polypropylene material, the specification of bacterium bag after charging: long 40cm-45cm, directly
Through 10cm-12cm.
The rectangular opening is the rectangular opening gone out with the jack machine transplanting of rice by disinfection.
By the bacterium bag, push-in sterilizing chamber sterilizes and is cooled to room temperature on sterilizing frame, the specification of the sterilizing frame: long
150cm, wide 43cm-48cm, high 100cm;Telescopic aseptic tunnel, the sleeve are equipped between the sterilizing chamber and transfer room
The bottom of formula aseptic tunnel is equipped with roller bearing, is equipped with ultraviolet sterilizing lamp and ion blower at top;By the bacterium bag after sterilizing together with
Sterilizing frame moves into transfer room by the telescopic aseptic tunnel and is inoculated with.
The beneficial effects of adopting the technical scheme are that this method vaccination is few, pollution probability is low;By chip
Strain has accessed the shaft centre line position of bacterial spawn, and the distance that strain diffusion grows into each position in bacterium bag is most suitable;This method exists
Vaccination tampon or sponge are sealed, and existing gas permeability can contact sufficient oxygen, bacterium germination speed again in mycelia growth course
Fastly, mycelia is non-aging;This method broken bag rate is small, can reduce labor intensity, and saves labour.
Specific embodiment
Embodiment 1: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously
It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening
It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle
Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big
In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The edible fungus species of the mushroom strain routine of the rectangle expand numerous technique and are made, and material is broadleaf tree, rule
Lattice: 12cm-18cm, wide 0.6cm-0.8cm are high
0.2cm-0.4cm。
Embodiment 2: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously
It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening
It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle
Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big
In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The bacterium bag is polyethylene or the dog-ear bag of polypropylene material, the specification of bacterium bag after charging: long 40cm-45cm, directly
Through 10cm-12cm.
Embodiment 3: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously
It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening
It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle
Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big
In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The rectangular opening is the rectangular opening gone out with the jack machine transplanting of rice by disinfection.
Embodiment 4: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously
It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening
It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle
Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big
In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
By the bacterium bag, push-in sterilizing chamber sterilizes and is cooled to room temperature on sterilizing frame, the specification of the sterilizing frame: long
150cm, wide 43cm-48cm, high 100cm;Telescopic aseptic tunnel, the sleeve are equipped between the sterilizing chamber and transfer room
The bottom of formula aseptic tunnel is equipped with roller bearing, is equipped with ultraviolet sterilizing lamp and ion blower at top;By the bacterium bag after sterilizing together with
Sterilizing frame moves into transfer room by the telescopic aseptic tunnel and is inoculated with.
Claims (5)
1. a kind of cultivating champignon inoculation method, it is characterised in that operate as follows: one, cultivating champignon culture medium is packed into bacterium
Bag, the bacterium bag form cylindrical body, and bacterium bag is put into sterilizing chamber and sterilizes and is cooled to room temperature;Two, bacterium bag is moved into transfer room
Inoculation;Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening interpolation
Enter the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the mushroom strain of rectangle
Length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than
The height 0.2cm-0.3cm of the mushroom strain of rectangle;Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
2. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the mushroom strain of the rectangle is used
Conventional edible fungus species expand numerous technique and are made, and material is broadleaf tree, specification: long 12cm-18cm, wide 0.6cm-0.8cm,
High 0.2cm-0.4cm.
3. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the bacterium bag is polyethylene or gathers
The dog-ear bag of propylene material, the specification of bacterium bag after charging: long 40cm-45cm, diameter 10cm-12cm.
4. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the rectangular opening is with by disappearing
The rectangular opening that the jack machine transplanting of rice of poison goes out.
5. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the bacterium bag is mounted in sterilizing frame
Upper push-in sterilizing chamber sterilizes and is cooled to room temperature, the specification of the sterilizing frame: long 150cm, wide 43cm-48cm, high 100cm;?
Telescopic aseptic tunnel is equipped between the sterilizing chamber and transfer room, the bottom of the telescopic aseptic tunnel is equipped with roller bearing,
Top is equipped with ultraviolet sterilizing lamp and ion blower;Bacterium bag after sterilizing is passed through into the telescopic sterile tunnel together with sterilizing frame
Road moves into transfer room and is inoculated with.
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102037855A (en) * | 2009-10-26 | 2011-05-04 | 杨青 | Three-dimensional cultivation technology for mushroom |
CN102648685A (en) * | 2012-04-28 | 2012-08-29 | 华南农业大学 | Chinese mushroom cultivation method and preparation method for special culture medium |
CN102986449A (en) * | 2012-08-21 | 2013-03-27 | 南阳市乾景中药材开发有限公司 | Method for improving mushroom quality |
CN103548565A (en) * | 2013-10-28 | 2014-02-05 | 河北省农业环境保护监测站 | Shiitake cultivating and inoculating method |
-
2017
- 2017-06-29 CN CN201710518398.1A patent/CN109418025A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102037855A (en) * | 2009-10-26 | 2011-05-04 | 杨青 | Three-dimensional cultivation technology for mushroom |
CN102648685A (en) * | 2012-04-28 | 2012-08-29 | 华南农业大学 | Chinese mushroom cultivation method and preparation method for special culture medium |
CN102986449A (en) * | 2012-08-21 | 2013-03-27 | 南阳市乾景中药材开发有限公司 | Method for improving mushroom quality |
CN103548565A (en) * | 2013-10-28 | 2014-02-05 | 河北省农业环境保护监测站 | Shiitake cultivating and inoculating method |
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Application publication date: 20190305 |