CN109418025A - A kind of cultivating champignon inoculation method - Google Patents

A kind of cultivating champignon inoculation method Download PDF

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Publication number
CN109418025A
CN109418025A CN201710518398.1A CN201710518398A CN109418025A CN 109418025 A CN109418025 A CN 109418025A CN 201710518398 A CN201710518398 A CN 201710518398A CN 109418025 A CN109418025 A CN 109418025A
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China
Prior art keywords
bacterium bag
rectangular opening
inoculation
rectangle
mushroom strain
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CN201710518398.1A
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Chinese (zh)
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祁海杰
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Individual
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Individual
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Abstract

The invention discloses a kind of cultivating champignon inoculation method, genus agaricus technical field of cultivation.It is characterized in that operating as follows: one, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber and sterilizes and is cooled to room temperature;Two, bacterium bag is moved into inoculation indoor inoculation;Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, the mushroom strain that section is rectangle is inserted into the rectangular opening, and sealed with tampon or sponge;The length of the rectangular opening is greater than the mushroom strain length 2cm-3cm of rectangle, and the width of rectangular opening is greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than the height 0.2cm-0.3cm of the mushroom strain of rectangle;Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.This method keeps strain and the culture medium contact spacing in bacterium bag suitable, and bacterium germination speed is fast, and inoculation efficiency is high.

Description

A kind of cultivating champignon inoculation method
Technical field
The present invention relates to mushroom-cultivating technical fields.
Background technique
Existing cultivating champignon inoculation method is that culture medium is packed into bacterium bag, swings in sterilizing indoor sterilizing, then again by bacterium Bag moves into cooling chamber, and when inoculation, which takes, beats cave inocalation method, i.e. bacterium bag is laid out station, got by hand on bacterium bag face by vaccinator The isometric circle cavities of 3-4 diameter 1.5cm, depth 6cm, with hand by Saw-dust break it is blocking fill in each cavities and compress, Then bagging or with adhesive tape seal inoculation cave, inoculation complete, then by bacterium bag move into bacteria room carry out Mycelium culture.The method Drawback is: pollution probability is high;The gap of strain and cultivating in a fungus bag base is big, and the bacterium germination time is long and inoculation efficiency is low.
Summary of the invention
This hair is the technical problem to be solved is that a kind of cultivating champignon inoculation method is provided, with the training in strain and bacterium bag Support the feature that base gap is suitable, bacterium germination speed is fast, mycelia is non-aging, inoculation efficiency is high.
In order to solve the above technical problems, the technical solution used in the present invention is: a kind of cultivating champignon inoculation method, special Sign is to operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The further improvement done to the present invention is:
The edible fungus species of the mushroom strain routine of the rectangle expand numerous technique and are made, and material is broadleaf tree, rule Lattice: 12cm-18cm, wide 0.6cm-0.8cm, 0.2cm-0.4cm.
The bacterium bag is polyethylene or the dog-ear bag of polypropylene material, the specification of bacterium bag after charging: long 40cm-45cm, directly Through 10cm-12cm.
The rectangular opening is the rectangular opening gone out with the jack machine transplanting of rice by disinfection.
By the bacterium bag, push-in sterilizing chamber sterilizes and is cooled to room temperature on sterilizing frame, the specification of the sterilizing frame: long 150cm, wide 43cm-48cm, high 100cm;Telescopic aseptic tunnel, the sleeve are equipped between the sterilizing chamber and transfer room The bottom of formula aseptic tunnel is equipped with roller bearing, is equipped with ultraviolet sterilizing lamp and ion blower at top;By the bacterium bag after sterilizing together with Sterilizing frame moves into transfer room by the telescopic aseptic tunnel and is inoculated with.
The beneficial effects of adopting the technical scheme are that this method vaccination is few, pollution probability is low;By chip Strain has accessed the shaft centre line position of bacterial spawn, and the distance that strain diffusion grows into each position in bacterium bag is most suitable;This method exists Vaccination tampon or sponge are sealed, and existing gas permeability can contact sufficient oxygen, bacterium germination speed again in mycelia growth course Fastly, mycelia is non-aging;This method broken bag rate is small, can reduce labor intensity, and saves labour.
Specific embodiment
Embodiment 1: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The edible fungus species of the mushroom strain routine of the rectangle expand numerous technique and are made, and material is broadleaf tree, rule Lattice: 12cm-18cm, wide 0.6cm-0.8cm are high
0.2cm-0.4cm。
Embodiment 2: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The bacterium bag is polyethylene or the dog-ear bag of polypropylene material, the specification of bacterium bag after charging: long 40cm-45cm, directly Through 10cm-12cm.
Embodiment 3: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
The rectangular opening is the rectangular opening gone out with the jack machine transplanting of rice by disinfection.
Embodiment 4: a kind of cultivating champignon inoculation method, it is characterised in that operate as follows:
One, cultivating champignon culture medium is packed into bacterium bag, the bacterium bag forms cylindrical body, and bacterium bag is put into sterilizing chamber's sterilizing simultaneously It is cooled to room temperature;
Two, bacterium bag is moved into inoculation indoor inoculation;
Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening It is inserted into the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the Lenlinus edodes of rectangle Kind length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is big In the height 0.2cm-0.3cm of the mushroom strain of rectangle;
Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
By the bacterium bag, push-in sterilizing chamber sterilizes and is cooled to room temperature on sterilizing frame, the specification of the sterilizing frame: long 150cm, wide 43cm-48cm, high 100cm;Telescopic aseptic tunnel, the sleeve are equipped between the sterilizing chamber and transfer room The bottom of formula aseptic tunnel is equipped with roller bearing, is equipped with ultraviolet sterilizing lamp and ion blower at top;By the bacterium bag after sterilizing together with Sterilizing frame moves into transfer room by the telescopic aseptic tunnel and is inoculated with.

Claims (5)

1. a kind of cultivating champignon inoculation method, it is characterised in that operate as follows: one, cultivating champignon culture medium is packed into bacterium Bag, the bacterium bag form cylindrical body, and bacterium bag is put into sterilizing chamber and sterilizes and is cooled to room temperature;Two, bacterium bag is moved into transfer room Inoculation;Three, in transfer room, the axial direction at the center of cylindrical body bacterium bag both ends of the surface beats rectangular opening, in the rectangular opening interpolation Enter the mushroom strain that section is rectangle, and is sealed with tampon or sponge;The length of the rectangular opening is greater than the mushroom strain of rectangle Length 2cm-3cm, the width of rectangular opening are greater than the width 0.2cm-0.3cm of the mushroom strain of rectangle, and the height of rectangular opening is greater than The height 0.2cm-0.3cm of the mushroom strain of rectangle;Four, the bacterium bag after inoculation is moved into bacteria room and carries out mycelium cultivation.
2. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the mushroom strain of the rectangle is used Conventional edible fungus species expand numerous technique and are made, and material is broadleaf tree, specification: long 12cm-18cm, wide 0.6cm-0.8cm, High 0.2cm-0.4cm.
3. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the bacterium bag is polyethylene or gathers The dog-ear bag of propylene material, the specification of bacterium bag after charging: long 40cm-45cm, diameter 10cm-12cm.
4. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the rectangular opening is with by disappearing The rectangular opening that the jack machine transplanting of rice of poison goes out.
5. a kind of cultivating champignon inoculation method according to claim 1, it is characterised in that: the bacterium bag is mounted in sterilizing frame Upper push-in sterilizing chamber sterilizes and is cooled to room temperature, the specification of the sterilizing frame: long 150cm, wide 43cm-48cm, high 100cm;? Telescopic aseptic tunnel is equipped between the sterilizing chamber and transfer room, the bottom of the telescopic aseptic tunnel is equipped with roller bearing, Top is equipped with ultraviolet sterilizing lamp and ion blower;Bacterium bag after sterilizing is passed through into the telescopic sterile tunnel together with sterilizing frame Road moves into transfer room and is inoculated with.
CN201710518398.1A 2017-06-29 2017-06-29 A kind of cultivating champignon inoculation method Pending CN109418025A (en)

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Application Number Priority Date Filing Date Title
CN201710518398.1A CN109418025A (en) 2017-06-29 2017-06-29 A kind of cultivating champignon inoculation method

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CN109418025A true CN109418025A (en) 2019-03-05

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102037855A (en) * 2009-10-26 2011-05-04 杨青 Three-dimensional cultivation technology for mushroom
CN102648685A (en) * 2012-04-28 2012-08-29 华南农业大学 Chinese mushroom cultivation method and preparation method for special culture medium
CN102986449A (en) * 2012-08-21 2013-03-27 南阳市乾景中药材开发有限公司 Method for improving mushroom quality
CN103548565A (en) * 2013-10-28 2014-02-05 河北省农业环境保护监测站 Shiitake cultivating and inoculating method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102037855A (en) * 2009-10-26 2011-05-04 杨青 Three-dimensional cultivation technology for mushroom
CN102648685A (en) * 2012-04-28 2012-08-29 华南农业大学 Chinese mushroom cultivation method and preparation method for special culture medium
CN102986449A (en) * 2012-08-21 2013-03-27 南阳市乾景中药材开发有限公司 Method for improving mushroom quality
CN103548565A (en) * 2013-10-28 2014-02-05 河北省农业环境保护监测站 Shiitake cultivating and inoculating method

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Application publication date: 20190305