KR0152692B1 - Method of cultivating phellinus linteus - Google Patents

Abstract

The present invention relates to a culture method of fungal mushrooms,

① The main materials of the medium are oak sawdust 30wt%, cottonwood sawdust 30wt%, mulberry sawdust 20wt% and rice bran (rice bran) 20wt%, and ground water is added to make the concentration 70% and mixed thoroughly. Filling the medium into a prepared bottle to fill the medium;

② Completely process the filled bottle for 1 to 2 hours at a temperature of 121 ℃ according to a known method in a high pressure sterilization pot or 6 to 8 hours at a temperature of 60 to 80 ℃ according to a known method in a normal sterilization pot. Sterilizing and inoculating the prepared S. mushrooms under sterile conditions and incubating at 25 ° C. for 60 days in a culture room;

③ Transfer the medium grown in the above to the cultivator, take out the cultured seedlings from the bottle, put it in a cultivated film bag with holes, and seal it, and then heat the film for 60 to 80 ° C. for 5 to 10 seconds according to a method known as a hot air blower. Shrinking slightly to make it taut;

④ culturing at 50-60 days with a temperature of 25-27 ° C. and a humidity of 70% in the cultivator to form a fungus;

⑤ removing the film by removing the situation mushroom fungi thickly formed by entangled mycelia;

According to this culture method, it is possible to artificially cultivate a large amount of situation mushroom with excellent anticancer effect.

Description

Bacterial Culture of Situation Mushroom

1 is a side view of the fungus cultured by the present invention,

2 is a cross-sectional view of the fungus cultured by the present invention,

3 is a cross-sectional view of a state in which the medium cultured according to the present invention is put into a shrink film bag and sealed.

* Explanation of symbols for main parts of the drawings

1: shrink film bag 2: perforation

3: spawn-like medium 4: shrink film bag inlet

The present invention relates to a method for cultivating fungi of S. mushrooms, and more specifically, to a method for cultivation of bacteria for artificial cultivation of S. mushrooms having an excellent anticancer effect.

Mushrooms have been growing wild in the field for edible and medicinal use, and the world's natural health food has been proven to be able to prevent various diseases and cancers, medically proved that the popularity of the mushroom is increasing day by day.

Reported to date, there have been academic reports that shiitake mushrooms have anti-cancer and antiviral effects, and blood cholesterol lowering effects.Amino acids, vitamins and proteins are also used in various mushrooms such as ganoderma lucidum, cloud mushroom, leaf mushroom, and agaricus mushroom. In addition, it contains a large amount of nutrients important to the human body such as inorganic salts, enzymes and sugars, as well as an ingredient called eritadenine, which reduces blood cholesterol.

Among them, the situational mushroom (Phellinus linteus, Korean name: woody mud mushroom), which showed extraordinary interest in the anti-cancer effects in Korea, Japan and China, was found to have a 96.7% tumor suppression rate. As a result, the demand of each country is soaring, and they are trying to grow the situation mushroom.

However, the situation mushrooms are widely distributed throughout the world, but the fruiting body is not well formed in nature, so it is difficult to obtain and the culture of the test tube is somewhat easy, but artificial cultivation is difficult.

Therefore, there is no clear artificial cultivation technology, and some research papers and some growers on the situation mushroom have been experimentally cultivated, but the results have not been achieved.

Accordingly, the present inventors have completed the present invention after intensive experiments and studies on artificial cultivation of situation mushrooms for many years in view of such circumstances.

That is, the situation mushroom fungal culture method in the present invention

① Main ingredients of oak sawdust 30wt%, cottonwood sawdust 30wt%, mulberry

20 wt% of sawdust and 20 wt% of rice bran (rice bran), and ground water is added thereto so as to have a concentration of 70%, and the mixture is completely mixed and packed into a prepared bottle by filling into a prepared bottle;

② Completely sterilize the filled bottle by treatment in a high pressure sterilization pot at a temperature of 121 ° C. for 1-2 hours or in a sterilization pot at a temperature of 60 to 80 ° C. for 6 to 8 hours. And inoculating the prepared S. aureus under aseptic conditions and incubating at 25 ° C. for 60 days in a culture room;

③ Transfer the medium grown in the above to the cultivator, take out the cultured seedlings from the bottle, put it in a cultivated film bag with holes, and seal it, and then heat the film for 60 to 80 ° C. for 5 to 10 seconds according to a method known as a hot air blower. Shrinking slightly to make it taut;

④ cultivation at 50-60 days with a temperature of 25-27 ° C. and a humidity of 70% in the cultivator to form a fungus;

⑤ removing the film by removing the situation mushroom fungi thickly formed by entangled mycelia;

It is made of.

Hereinafter, each process step of the present invention will be described in detail.

(1) medium formation step

Groundwater is added to the above-mentioned sawdust material, measured with a moisture meter to form a well-mixed medium while maintaining moisture of approximately 70% or more, and filled into a prepared bottle with 1 kg of a known bottle, where space between sawdust Recharge well to avoid filling.

In addition, the bottle spout should be cleaned and faced with cotton so that the mycelium in the medium can grow well by air circulation and prevent infiltration of various germs.

(2) Sterilization, culture stage

Due to the moisture added and mixed in the medium, various molds in the medium grow and generate heat, and as the mold continues to multiply, toxins are released, and the mushroom bacteria cannot grow, but only molds that can withstand this toxin. In order to grow only the desired mushrooms, it is necessary to heat them quickly before the fungus grows and sterilize them to make a medium that only kills all the unwanted bacteria and inoculates them.

The following two known sterilization methods can be applied to the sterilization method.

One method is to apply high pressure sterilization method using the autoclave boiler, and put the mixed medium into the sterilization pot so that the pressure is not tight and the pot can withstand the internal pressure of 3㎏ / ㎠ or more. Should be.

At first, the pressure is gradually increased by the boiler, and the steam is released to the outside until the temperature reaches 70 ~ 80 ℃, and the cold air inside is also flowed out. If the temperature continues to rise, the exhaust valve is locked to increase the pressure. If the pressure is kept at a temperature of 121 ° C. at 1 kg / cm 2 for 1 to 2 hours, and the temperature is lowered to 0 kg / cm 2 or lower, the temperature is lowered to 80 ° C. or lower. The temperature of is lowered to 20-25 ° C.

In another method, atmospheric sterilization is a method in which a mixed medium is heated in a direct heat or steam manner, and the medium is placed in a pot and heated at a temperature of 60 to 80 ° C. for at least 6 to 8 hours, and then the temperature is lowered. When the temperature is 20-25 ℃ send to the inoculation room.

After sterilization of the fungus by either of the above two methods, the prepared seed is inoculated under a sterile state in which the air of the inoculation box is purified by using a HEPA filter in the inoculation room to completely remove other germs (almost 99%).

(3) Film wrapping step in cultivator (see Figure 3)

Transfer the grown medium to the grower, remove the spawn bottle, and put the spawn bottle-shaped medium (3) into the shrink film bag (1) that is about 1 cm larger than the spawn bottle. Sealing using the instant adhesive, when the heat is applied to the sealed bag (1) with a heat blower, the shrink film bag (1) is shrunk and adheres to the spawn bottle-shaped medium (3) without space. As shown in Table 1 below, when the distance between the hot air blower and the shrink film bag is 10 cm, the shrinkage is completed in about 5 seconds at a temperature of 80 ° C., and the light bag is slightly applied at about 60 seconds at 60 ° C. Contracts. At this time, in order to speed up the work, when the distance between the hot air blower and the shrink film bag is 5 cm at a temperature of 80 ° C., the shrinkage work can be performed in a short time within 1 second.

(The hot air used in the experiment was made in Japan and used AC 100V 50-60HZ, 700W with National's EH789, the maximum temperature was 183 ℃, and the hot air emission standard was 9㎠; the shrink film bag was produced by Yeonwoo Chemical Co., Ltd. Experimented with a prototype that can shrink 10% at 60 ℃ at a distance of 25cm in mm.)

(4) step of forming fungus

As shown in FIG. 3, the perforated 2 is formed in the fully shrunk film bag so that oxygen is supplied thereto, and the bacterium is vigorously generated at the perforated part 2.

If the fungus grows vigorously in the perforated part, the oxygen supply is insufficient and the growth of the fungus stops, so the pores can be enlarged and grow more. It may be.

According to the bacterium culture method of the situation mushroom of the present invention configured as described above, it is possible to completely artificially cultivate the precious situation mushroom by completely eliminating the point that artificial cultivation was impossible because the conventional fruiting body was not well formed, which would greatly contribute to farm income. In addition, the supply of large amounts to help cancer patients and various diseases, as well as to improve the health of the general public will have a significant effect in the supply and demand of situation mushrooms.

Claims (1)

In the bacterium culture method of the situation mushroom, ① the main material of the medium is oak sawdust 30wt%, cottonwood sawdust 30wt%, mulberry sawdust 20wt% and rice bran 20wt% and ground water is added to make 70% concentration. Mixing and filling the mixed medium into a prepared bottle to fill; ② Completely process the filled bottle for 1 to 2 hours at a temperature of 121 ℃ according to a known method in a high pressure sterilization pot or 6 to 8 hours at a temperature of 60 to 80 ℃ according to a known method in a normal sterilization pot. Sterilizing and inoculating the prepared S. mushrooms under sterile conditions and incubating at 25 ° C. for 60 days in a culture room; ③ Remove the cultured seedlings from the bottle, which has been grown in the medium, grown in the above-mentioned medium, and put it in a cultivated film bag which is perforated and sealed, and then heat the film for 60 to 80 ° C. for 5 to 10 seconds according to a method known as a hot air blower. Shrinking slightly to make it taut; ④ cultivation at 50-60 days with a temperature of 25-27 ° C. and a humidity of 70% in the cultivator to form a fungus; ⑤ removing the film by removing the situation mushroom fungi thickly formed by entangled mycelia; The fungal culture method of the situation mushroom, characterized in that consisting of.