CN206328381U - A kind of cell filter - Google Patents
A kind of cell filter Download PDFInfo
- Publication number
- CN206328381U CN206328381U CN201621469718.6U CN201621469718U CN206328381U CN 206328381 U CN206328381 U CN 206328381U CN 201621469718 U CN201621469718 U CN 201621469718U CN 206328381 U CN206328381 U CN 206328381U
- Authority
- CN
- China
- Prior art keywords
- collector
- filtering area
- cell filter
- cell
- filter according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model is related to a kind of cell filter, including a shape in cylinder upper collector and with the upper integrally formed filtering area in collector lower end, the upper collector upper end is provided with lid, the upper collector and filtering area connectivity part are provided with a valve, and the filtering area bottom is provided with filter membrane；Also include lower collector, the lower collector is detachably connected with the filtering area lower end.The structure of the utility model cell filter makes piping and druming, filtering, collects disposable completion, easy to operate, reduces the possibility of pollution, more meets sterility requirements, its simplicity and tight aseptic design for operating can provide more convenient experiment condition for experimenter.
Description
Technical field
The utility model is related to a kind of medical treatment and labware, and in particular to a kind of cell filter.
Background technology
Cell primary culture is one of biology and the most frequently used means of medical research, and cell primary culture is directly from life
Object acquisition cell, tissue and organ are cultivated the cultivation stage to before passage.Because cell is just from biological tissue point
Separate out and, therefore closer to the animation in organism, this method can be the growth of research biological cell, metabolism, numerous
Grow and strong means are provided, while also being created conditions for later Secondary Culture, can also be directly served in using the method clinical real
Trample.Separate, digested after tissue in animal body, except primary cell, typically also there is the original structure do not scattered completely, device
Official, cell mass and nutrient solution, so tentatively being filtered after needing first to blow and beat mixed liquor in the collection of product, to mixed liquor
Filtering need to use cell filter, cell filter can complete magazine filtering, scattered, point sample of cell etc..
Cell filter provides a kind of method simple and easy to apply for dixie cup single cell suspension.Existing cell filter leads to
The screen net for crossing its special pore size distribution having is filtered, wherein needing the cell disposable cell screen net/metal material
Screen net draws the cell suspending liquid after piping and druming with suction pipe, drips and filtered in screen net on centrifuge tube, then after filtering
Cell suspending liquid draw, collect, be inoculated with culture dish.
Each step relative separation of the above, especially complex operation, the sterile working to lab assistant require higher；And mistake
The normal Reusability after autoclaving of the cell screen net for the metal material used during filter, gets rusty easily after, causes long
Pollution；For the cell screen net of metal material, also have to remove by using the cleaning of diluted alkaline water in the prior art and remained above screen
Cell and tissue, then use detergent solution soaking and washing, finally rinse net screen, distilled water, redistilled water punching with running water
Wash, screen and packaging sterilizing processing, then screen is put into preservation, the cleaning way complex steps, waste of manpower in packaging.
Utility model content
In view of this, the purpose of this utility model is to overcome the deficiencies in the prior art there is provided a kind of cell of integral type
Filter, makes piping and druming, filtering, collects disposable completion, easy to operate, reduces the possibility of pollution, more meets sterility requirements.
To realize object above, the utility model is adopted the following technical scheme that：A kind of cell filter, including a shape is in
The upper collector of cylinder and with the upper integrally formed filtering area in collector lower end, the upper collector upper end provided with lid
Son, upper collector and the filtering area connectivity part is provided with a valve, and the filtering area bottom is provided with filter membrane；Also include lower collect
Device, the lower collector is detachably connected with the filtering area lower end.
Preferably, the internal thread on the inside of the lid and the external screw thread of upper collector one end outer surface are connected by screw thread
Connect.
Preferably, the filtering area is either interference fitted with the connected mode of lower collector to be threadedly coupled, filling in envelope, i.e.,
The external screw thread in the filtering area lower end outside face is connected with the internal thread of the lower collector upper end medial surface, or the mistake
The upper end of the lower collector, or interference fit are filled in the sealing of filter area lower end, and using expanding with heat and contract with cold, characteristic will the lower receipts
The upper end of storage is set to the lower end of the filtering area.
Preferably, the lower collector is shaped as cylinder.
Preferably, the diameter of the upper collector and lower collector is equal to the diameter of 50ml centrifuge tubes.
Preferably, the length of the upper collector is less than the length of 50ml centrifuge tubes.
Preferably, the filter membrane uses nylon screen structure, and the cell filter uses plastics material in addition to filter membrane
Matter.
Preferably, the valve is Medical check valve.
Preferably, the filtering area is shaped as cylinder either centrum.
The utility model uses above technical scheme, and upper collector more facilitates during cell piping and druming than culture dish,
It is difficult to overflow, and compared with 50ml centrifuge tubes, eliminates unnecessary length, saves material；The sealing of intervening vaive ensure that
The internal aseptic of device and separate up and down；Upper collector and lower collector diameter are identical with 50ml centrifuge tubes, can will be thin when using
Born of the same parents' filter is inserted directly into rack for test tube, is easy to operation；Filtering area is detachably connected mode with lower collector, both ensures cell
Filter interior it is sterile, lower collector can be easily removed again, be easy to collect filter after liquid.The utility model cell mistake
The structure of filter makes piping and druming, filtering, the disposable completion of collection, easy to operate, reduces the possibility of pollution, more meets sterile want
Ask, its simplicity and tight aseptic design for operating can provide more convenient experiment condition for experimenter.
Brief description of the drawings
Fig. 1 is the utility model cell filter schematic diagram；
Fig. 2 is the utility model cell filter profile.
In figure：1st, lid；2nd, upper collector；3rd, valve；4th, filter membrane；5th, lower collector；6th, filtering area.
Embodiment
, below will be to technology of the present utility model to make the purpose of this utility model, technical scheme and advantage clearer
Scheme is described in detail.Obviously, described embodiment is only a part of embodiment of the utility model, rather than all
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not before creative work is made
Resulting all other embodiment is put, the scope that the utility model is protected is belonged to.
Embodiment one, as depicted in figs. 1 and 2, a kind of cell filter, including a shape are in the upper collector 2 of cylinder
And with the upper integrally formed filtering area 6 in the lower end of collector 2, the upper upper end of collector 2 be provided with lid 1, it is described it is upper receive
Storage 2 and the connectivity part of filtering area 6 are provided with a valve 3, and the bottom of filtering area 6 is provided with filter membrane 4；Also include lower collector 5, it is described
Lower collector 5 is detachably connected with the lower end of filtering area 6.
Embodiment two, a kind of cell filter, including a shape in cylinder upper collector 2 and with it is described it is upper collect
The integrally formed filtering area 6 in the lower end of device 2, the upper upper end of collector 2 is provided with lid 1, and the upper collector 2 and filtering area 6 connect
Logical place is provided with a valve 3, and the bottom of filtering area 6 is provided with filter membrane 4；
Also include lower collector 5, the lower collector 5 is detachably connected with the lower end of filtering area 6.In the lid 1
The internal thread of side and the external screw thread of the upper one end outer surface of collector 2 are connected through a screw thread.The filtering area 6 and lower collector
5 connected mode is threaded connection, plug envelope is either interference fitted, i.e., the external screw thread in the described lower end outside face of filtering area 6 and institute
The internal thread connection of the lower upper end medial surface of collector 5 is stated, or the lower collector 5 is filled in the sealing of the lower end of the filtering area 6
Upper end, or interference fit, the upper end of the lower collector 5 is set to the filtering area 6 using characteristic of expanding with heat and contract with cold
Lower end.
In the present embodiment, the lower collector 5 is shaped as cylinder.
It should be noted that the diameter of the upper collector 2 and lower collector 5 is equal to the diameter of 50ml centrifuge tubes, it is described
The length of upper collector 2 is less than the length of 50ml centrifuge tubes.
It should be added that, the filter membrane 4 uses nylon screen structure, and the cell filter is in addition to filter membrane 4
Use plastic material.
It is understood that the valve 3 is Medical check valve.
In addition, the filtering area 6 is shaped as cylinder either centrum.
When actually used, 1 is twisted the lid off first, bone marrow fluid irrigation with syringe is entered in upper collector 2, with disposable drop
Pipe blows and beats bone marrow cell uniformly in upper collector 2, then opens valve 3, cell suspending liquid enters filtering area 6, through filter membrane 4
Lower collector 5 is flowed into after filtering, lower collector 5 is backed out, the single cell suspension after filtering is drawn, planted into blake bottle/culture
In ware.It can be seen that, piping and druming, filtering can be made using the utility model cell filter, disposable completion is collected, it is easy to operate, reduce
The possibility of pollution, more meets sterility requirements.
The utility model uses above technical scheme, and upper collector more facilitates during cell piping and druming than culture dish,
It is difficult to overflow, and compared with 50ml centrifuge tubes, eliminates unnecessary length, saves material；The sealing of intervening vaive ensure that
The internal aseptic of device and separate up and down；Filtering area is detachably connected mode with lower collector, both ensures in cell filter
Portion it is sterile, lower collector can be easily removed again, be easy to collect filter after liquid.The knot of the utility model cell filter
Structure makes piping and druming, filtering, collects disposable completion, easy to operate, reduces the possibility of pollution, more meets sterility requirements, it is operated
Simplicity and tight aseptic design, more convenient experiment condition can be provided for experimenter.
The utility model is not limited to above-mentioned preferred forms, and anyone can draw under enlightenment of the present utility model
Other various forms of products, however, make any change in its shape or structure, it is every that there is same as the present application or phase
Approximate technical scheme, all falls within protection domain of the present utility model.
Claims (10)
1. a kind of cell filter, it is characterised in that upper collected in the upper collector of cylinder and with described including a shape
The integrally formed filtering area in device lower end, the upper collector upper end is provided with lid, and upper collector and the filtering area connectivity part is set
There is a valve, the filtering area bottom is provided with filter membrane；Also include lower collector, the lower collector can with the filtering area lower end
Dismounting connection.
2. cell filter according to claim 1, it is characterised in that internal thread and the upper receipts on the inside of the lid
The external screw thread of storage one end outer surface is connected through a screw thread.
3. cell filter according to claim 1, it is characterised in that lower collector and the filtering area lower end it is removable
Mode is unloaded for threaded connection, plug envelope or is interference fitted.
4. cell filter according to claim 1, it is characterised in that the lower collector is shaped as cylinder.
5. cell filter according to claim 4, it is characterised in that diameter of the upper collector and lower collector etc.
In the diameter of 50ml centrifuge tubes.
6. cell filter according to claim 1, it is characterised in that the length of the upper collector is centrifuged less than 50ml
The length of pipe.
7. the cell filter according to any one of claim 1 to 6, it is characterised in that the filter membrane uses nylon screen
Structure.
8. the cell filter according to any one of claim 1 to 6, it is characterised in that the cell filter removes filter membrane
Outside use plastic material.
9. the cell filter according to any one of claim 1 to 6, it is characterised in that the valve is Medical check valve.
10. the cell filter according to any one of claim 1 to 6, it is characterised in that the filtering area is shaped as cylinder
Body either centrum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621469718.6U CN206328381U (en) | 2016-12-29 | 2016-12-29 | A kind of cell filter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621469718.6U CN206328381U (en) | 2016-12-29 | 2016-12-29 | A kind of cell filter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206328381U true CN206328381U (en) | 2017-07-14 |
Family
ID=59292822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621469718.6U Expired - Fee Related CN206328381U (en) | 2016-12-29 | 2016-12-29 | A kind of cell filter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206328381U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479869A (en) * | 2016-12-29 | 2017-03-08 | 山东省职业卫生与职业病防治研究院 | A kind of cell filter |
-
2016
- 2016-12-29 CN CN201621469718.6U patent/CN206328381U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479869A (en) * | 2016-12-29 | 2017-03-08 | 山东省职业卫生与职业病防治研究院 | A kind of cell filter |
| CN114011245A (en) * | 2016-12-29 | 2022-02-08 | 山东省职业卫生与职业病防治研究院 | Cell filter and filtering method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170714 Termination date: 20201229 |