CN207210453U - A kind of Tissue Culture Flask - Google Patents
A kind of Tissue Culture Flask Download PDFInfo
- Publication number
- CN207210453U CN207210453U CN201720930001.5U CN201720930001U CN207210453U CN 207210453 U CN207210453 U CN 207210453U CN 201720930001 U CN201720930001 U CN 201720930001U CN 207210453 U CN207210453 U CN 207210453U
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- Prior art keywords
- bottle
- tube
- culture bottle
- culture
- sealing
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Abstract
The utility model provides a kind of Tissue Culture Flask, including cultivating bottle, the culture bottle is square cavity structure, two bottom sides are symmetrical arranged arc in the blake bottle body, the arc concave surface is away from the culture bottle bottom surface, the culture bottle body bottom sets rubber slab, and described culture bottle one end side wall center position sets the feed tube connected with the blake bottle body phase, and the angle between the feed tube and the culture bottle side wall is in 45 degree.The beneficial effects of the utility model are：In cell culture, it directly can carry out changing liquid by liquid exchange opening, culture bottle need not be tilted, so as to avoid cultivating bottle because tilting, cell departs from the problem of cultivating wall under gravity, it is more beneficial for the adherent growth of cell, meanwhile nutrient solution is more easily removed, leave no residue, and ensure the state of whole sealing during nutrient solution addition and cell extraction, ensure that cell culture is free from the influence of the external environment.
Description
Technical field
Biomedical culture device technical field is the utility model is related to, it particularly relates to a kind of Tissue Culture Flask.
Background technology
The in vitro culture of cell and tissue has turned into the essential content of life studies and practice, in regenerative medicine and disease
Also played an important role in disease treatment.The cell type of culture is various, from virus to bacterium and fungi, from human body cell to dynamic
Thing cell and plant cell.Some cells and tissue can grow under suspended state, but quite a few mammalian cell needs
Want surface adherent.At present, Tissue Culture Flask is the cuboid accommodating chamber that end has a bottleneck, generally by training when cultivating cell
Foster bottle, which fell, makes the one side (i.e. placed side) of its area maximum be contacted with desktop, to provide the adherent area of abundance for cell, when
When cell culture medium changes liquid, often Tissue Culture Flask is stood up or tilted, nutrient solution is poured out from bottleneck, and tissue block can be because
Depart from adherent surface for Action of Gravity Field, be unfavorable for the adherent growth of cell, and present Tissue Culture Flask generally existing is difficult
With extraction, the problem of extracting dead angle is easily produced.
The problem of in correlation technique, effective solution is not yet proposed at present.
Utility model content
The problem of in correlation technique, the utility model proposes a kind of Tissue Culture Flask, to solve above-mentioned background technology
The problem of middle proposition.
What the technical solution of the utility model was realized in：
A kind of Tissue Culture Flask, including culture bottle, the culture bottle is square cavity structure, the culture bottle
Inner bottom part both sides are symmetrical arranged arc, and the arc concave surface is away from the culture bottle bottom surface, the culture bottle body bottom
Rubber slab is set, and described culture bottle one end side wall center position sets the feed tube connected with the blake bottle body phase, institute
The angle stated between feed tube and the culture bottle side wall is in 45 degree, and the one end of the feed tube away from the culture bottle leads to
Cross and be threaded with bottle cap, the feed liquor inside pipe wall is remote close to one end setting deflector for cultivating bottle, the deflector
One end from the feed liquor inside pipe wall is stretched into inside the blake bottle body, and culture bottle upper surface center position, which is set, to be passed through
The liquid exchange opening of the culture bottle upper surface is worn, is set above the liquid exchange opening and changes liquid pipe, described change sets sealing at the top of liquid pipe
Plug, the sealing-plug and described change between liquid pipe inwall set the first sealing ring, and sealing-plug upper surface side sets insertion
The liquid flowing tube of the sealing-plug, the liquid flowing tube top set control valve, dismountable connecting tube are set at the top of the control valve,
One end connection Liquid absorption ball of the connecting tube away from the control valve, the liquid flowing tube and one end inside the sealing-plug
Horizontal tube is connected, one end connection VERTICAL TUBE of the horizontal tube away from the liquid flowing tube, the VERTICAL TUBE is away from the horizontal tube
One end through the sealing-plug bottom and extend to the culture bottle body bottom, the liquid flowing tube outer wall and the sealing-plug it
Between the second sealing ring is set, between the liquid flowing tube, horizontal tube and VERTICAL TUBE be interconnected.
Further, the culture bottle is made up of transparent material.
Further, the rubber slab covering culture bottle bottom surface, and the rubber slab bottom sets anti-slip veins.
Further, anti-skid bulge is set on the bottle cap wall.
Further, the deflector is parallel with the culture bottle bottom surface.
Further, culture bottle upper surface one end, the Bottle cap top center and the sealing-plug upper table
Side of the face away from the liquid flowing tube is respectively provided with label patch.
Further, at the top of the VERTICAL TUBE at the sealing-plug bottom centre position.
The beneficial effects of the utility model are：By the symmetrical arc of two bottom sides in blake bottle body, in culture bottle
Bottom sets rubber slab, and culture bottle one end side wall center position sets the feed liquor with bottle cap connected with blake bottle body phase
Pipe, feed tube are in 45 degree of angles with culture bottle side wall, and feed liquor inside pipe wall sets parallel with culture bottle bottom surface and stretches into training
The deflector inside bottle is supported, culture bottle upper surface center position sets the liquid exchange opening through culture bottle upper surface, changed
The liquid pipe of changing of sealing-plug is provided with above liquid mouth, sealing-plug upper surface side sets the liquid flowing tube of insertion sealing-plug, liquid flowing tube
Top sets control valve, and connecting tube is set at the top of control valve, and connection pipe end sets Liquid absorption ball, and liquid flowing tube is located inside sealing-plug
One end connection horizontal tube, the VERTICAL TUBE inside horizontal pipe end connection insertion blake bottle body, liquid flowing tube, horizontal tube and VERTICAL TUBE
Between be interconnected, so as in cell culture, can directly carry out changing liquid by liquid exchange opening, bottle is cultivated without tilting, so as to
Avoid cultivating bottle because tilting, cell departs from the problem of cultivating wall under gravity, is more beneficial for the adherent growth of cell, together
When, nutrient solution is more easily removed, is left no residue, and ensures whole sealing during nutrient solution addition and cell extraction
State, ensure cell culture it is free from the influence of the external environment.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
In the required accompanying drawing used be briefly described, it should be apparent that, drawings in the following description are only of the present utility model
Some embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other accompanying drawings.
Fig. 1 is the structural representation according to the Tissue Culture Flask of the utility model embodiment；
Fig. 2 is the structure top view according to the Tissue Culture Flask of the utility model embodiment.
In figure：
1st, bottle is cultivated；2nd, arc；3rd, rubber slab；4th, feed tube；5th, bottle cap；6th, deflector；7th, liquid exchange opening；8th, liquid is changed
Pipe；9th, sealing-plug；10th, the first sealing ring；11st, liquid flowing tube；12nd, control valve；13rd, connecting tube；14th, Liquid absorption ball；15th, horizontal tube；
16th, VERTICAL TUBE；17th, the second sealing ring；18th, anti-slip veins；19th, anti-skid bulge；20th, label pastes.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, every other embodiment that those of ordinary skill in the art are obtained all belongs to
In the scope of the utility model protection.
According to embodiment of the present utility model, there is provided a kind of Tissue Culture Flask.
As shown in Figure 1-2, according to the Tissue Culture Flask of the utility model embodiment, including culture bottle 1, the blake bottle
Body 1 is square cavity structure, and the culture bottle 1 inner bottom part both sides are symmetrical arranged arc 2, and the concave surface of arc 2 is remote
From culture bottle 1 bottom surface, culture bottle 1 bottom sets rubber slab 3, the culture one end side wall centre bit of bottle 1
The place of putting sets the feed tube 4 being connected with the culture bottle 1, the folder between the feed tube 4 and the culture side wall of bottle 1
Angle is in 45 degree, and the feed tube 4 has been connected through a screw thread bottle cap 5 away from described one end for cultivating bottle 1, in the feed tube 4
Wall sets deflector 6 close to one end of the culture bottle 1, and the one end of the deflector 6 away from the inwall of feed tube 4 is stretched into
Inside the culture bottle 1, the upper surface center position of culture bottle 1 is set through culture bottle 1 upper surface
Liquid exchange opening 7, the top of liquid exchange opening 7, which is set, changes liquid pipe 8, and the top of liquid pipe 8 of changing sets sealing-plug 9, the sealing-plug 9 and institute
State to change between the inwall of liquid pipe 8 and first sealing ring 10 is set, the upper surface side of sealing-plug 9, which is set, inserts the sealing-plug 9
Liquid flowing tube 11, the top of liquid flowing tube 11 set control valve 12, and the top of control valve 12 sets dismountable connecting tube 13, institute
State one end connection Liquid absorption ball 14 of the connecting tube 13 away from the control valve 12, the liquid flowing tube 11 and in the sealing-plug 9
One end connection horizontal tube 15 in portion, one end connection VERTICAL TUBE 16 of the horizontal tube 15 away from the liquid flowing tube 11 are described vertical
The one end of pipe 16 away from the horizontal tube 15 is through the bottom of sealing-plug 9 and extends to culture bottle 1 bottom, described to take
Second sealing ring 17 is set between the outer wall of liquid pipe 11 and the sealing-plug 9, the liquid flowing tube 11, horizontal tube 15 and VERTICAL TUBE 16 it
Between be interconnected.
In one embodiment, the culture bottle 1 is made up of transparent material.
In one embodiment, the rubber slab 3 covers culture bottle 1 bottom surface, and the bottom of the rubber slab 3 is set
Anti-slip veins 18.
In one embodiment, the outer wall of bottle cap 5 sets anti-skid bulge 19.
In one embodiment, the deflector 6 is parallel with culture bottle 1 bottom surface.
In one embodiment, described culture bottle 1 upper surface one end, the top center of the bottle cap 5 and described close
Side of the upper surface of blocking 9 away from the liquid flowing tube 11 is respectively provided with label patch 20.
In one embodiment, the top of VERTICAL TUBE 16 is at the bottom centre position of sealing-plug 9.
In summary, the bottom in culture bottle 1 is passed through by means of above-mentioned technical proposal of the present utility model, the utility model
The symmetrical arc 2 in portion both sides, rubber slab 3 is set in culture bottle 1 bottom, and one end side wall center position of culture bottle 1 is set
With the feed tube 4 with bottle cap 5 that is connected of culture bottle 1, feed tube 4 is in 45 degree of angles with cultivating the side wall of bottle 1, feed tube
4 inwalls set deflector 6 that is parallel with culture bottle 1 bottom surface and stretching into inside culture bottle 1, cultivate in the upper surface of bottle 1
Heart opening position sets the liquid exchange opening 7 through culture bottle 1 upper surface, and sealing-plug 9 is provided with above liquid exchange opening 7 changes liquid pipe 8,
The upper surface side of sealing-plug 9 sets the liquid flowing tube 11 of insertion sealing-plug 9, and the top of liquid flowing tube 11 sets control valve 12, control valve 12
Top sets connecting tube 13, and the end of connecting tube 13 sets Liquid absorption ball 14, and liquid flowing tube 11 is located at one end connection water inside sealing-plug 9
The VERTICAL TUBE 16 inside bottle 1, liquid flowing tube 11, horizontal tube 15 and VERTICAL TUBE are cultivated in flat pipe 15, the connection insertion of the end of horizontal tube 15
It is interconnected between 16, so as in cell culture, directly carry out changing liquid by liquid exchange opening 7, bottle 1 is cultivated without tilting,
So as to avoid cultivating bottle because tilting, cell departs from the problem of cultivating wall, is more beneficial for the adherent life of cell under gravity
It is long, meanwhile, it is more convenient to remove nutrient solution, leave no residue, and ensure entirely during nutrient solution addition and cell extraction
The state of journey sealing, ensure that cell culture is free from the influence of the external environment.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model
Within the spirit and principle of utility model, any modification, equivalent substitution and improvements made etc., the utility model should be included in
Protection domain within.
Claims (7)
1. a kind of Tissue Culture Flask, including culture bottle (1), it is characterised in that the culture bottle (1) is square cavity knot
Structure, described culture bottle (1) inner bottom part both sides are symmetrical arranged arc (2), and arc (2) concave surface is away from the blake bottle
Body (1) bottom surface, described culture bottle (1) bottom set rubber slab (3), culture bottle (1) one end side wall center position
The feed tube (4) being connected with the culture bottle (1) is set, between the feed tube (4) and described culture bottle (1) side wall
Angle be in 45 degree, the feed tube (4) away from it is described cultivate bottle (1) one end be connected through a screw thread bottle cap (5), it is described
Feed tube (4) inwall sets deflector (6) close to one end of the culture bottle (1), and the deflector (6) is away from the feed liquor
Culture bottle (1) inside is stretched into one end of pipe (4) inwall, and culture bottle (1) upper surface center position, which is set, to be passed through
The liquid exchange opening (7) of described culture bottle (1) upper surface is worn, is set above the liquid exchange opening (7) and changes liquid pipe (8), it is described to change liquid pipe
(8) top sets sealing-plug (9), and the sealing-plug (9) and described change between liquid pipe (8) inwall set the first sealing ring (10),
Sealing-plug (9) the upper surface side sets the liquid flowing tube (11) for inserting the sealing-plug (9), is set at the top of the liquid flowing tube (11)
Control valve (12) is put, dismountable connecting tube (13) is set at the top of the control valve (12), and the connecting tube (13) is away from described
One end connection Liquid absorption ball (14) of control valve (12), the liquid flowing tube (11) and one end company for being located at the sealing-plug (9) inside
Connect horizontal tube (15), one end connection VERTICAL TUBE (16) of the horizontal tube (15) away from the liquid flowing tube (11), the VERTICAL TUBE
(16) one end away from the horizontal tube (15) through the sealing-plug (9) bottom and extends to described culture bottle (1) bottom,
Second sealing ring (17), the liquid flowing tube (11), horizontal tube are set between liquid flowing tube (11) outer wall and the sealing-plug (9)
(15) it is interconnected between VERTICAL TUBE (16).
2. a kind of Tissue Culture Flask according to claim 1, it is characterised in that the culture bottle (1) is transparent material
It is made.
3. a kind of Tissue Culture Flask according to claim 1, it is characterised in that the rubber slab (3) covers the culture
Bottle (1) bottom surface, and the rubber slab (3) bottom sets anti-slip veins (18).
4. a kind of Tissue Culture Flask according to claim 1, it is characterised in that bottle cap (5) outer wall sets anti-skidding convex
Rise (19).
5. a kind of Tissue Culture Flask according to claim 1, it is characterised in that the deflector (6) and the blake bottle
Body (1) bottom surface is parallel.
A kind of 6. Tissue Culture Flask according to claim 1, it is characterised in that described culture bottle (1) upper surface one end,
The side of bottle cap (5) top center and the sealing-plug (9) upper surface away from the liquid flowing tube (11) is respectively provided with mark
Label patch (20).
7. a kind of Tissue Culture Flask according to claim 1, it is characterised in that positioned at described at the top of the VERTICAL TUBE (16)
At sealing-plug (9) bottom centre position.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720930001.5U CN207210453U (en) | 2017-07-28 | 2017-07-28 | A kind of Tissue Culture Flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720930001.5U CN207210453U (en) | 2017-07-28 | 2017-07-28 | A kind of Tissue Culture Flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207210453U true CN207210453U (en) | 2018-04-10 |
Family
ID=61814562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720930001.5U Expired - Fee Related CN207210453U (en) | 2017-07-28 | 2017-07-28 | A kind of Tissue Culture Flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207210453U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
-
2017
- 2017-07-28 CN CN201720930001.5U patent/CN207210453U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180410 Termination date: 20210728 |