CN116179352A - Full-automatic stem cell culture equipment - Google Patents
Full-automatic stem cell culture equipment Download PDFInfo
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- CN116179352A CN116179352A CN202310393862.4A CN202310393862A CN116179352A CN 116179352 A CN116179352 A CN 116179352A CN 202310393862 A CN202310393862 A CN 202310393862A CN 116179352 A CN116179352 A CN 116179352A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
- B25J—MANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
- B25J11/00—Manipulators not otherwise provided for
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract
The invention discloses full-automatic stem cell culture equipment, which comprises an equipment outer box, a box door, a cell culture box, an inlet box, an outlet box, an ozone disinfection cabinet, a transplanter, a dustbin, a centrifugal machine and a normal-temperature test tube bin, wherein a culture platform is arranged in the equipment outer box, the cell culture box is arranged on the surface of one side of the inside of the equipment outer box, seven cell culture boxes are arranged, an intelligent microscope is arranged between the seven cell culture boxes, a transplanter is arranged on one side of the bottom end of the inside of the equipment outer box, the dustbin is arranged at the lower end of the transplanter, the centrifugal machine is arranged on one side of the transplanter, and a special culture dish is arranged at one end of the transplanter.
Description
Technical Field
The invention belongs to the technical field of stem cell culture, and particularly relates to full-automatic stem cell culture equipment.
Background
Stem cells, in a simple sense, are a class of cells with unlimited or immortalized self-renewing capacity, capable of producing at least one type of highly differentiated daughter cells.
The definition of stem cells has been modified over the years and is defined from different levels. Most biologists and medical practitioners consider stem cells to be a class of cells from the embryo, fetus or adult body that have unlimited self-renewal and proliferation differentiation under certain conditions, and are able to produce daughter cells of the exact same phenotype as genotype and self, and also to produce specialized cells that make up tissues and organs of the body, while also differentiating into progenitor cells.
The university of belgium, luwen, research team uses stem cells in the laboratory to generate a new human cell.
Stem cells are a type of pluripotent cells with self-replication ability, which can differentiate into various functional cells under certain conditions, and are classified into embryonic stem cells and adult stem cells according to the developmental stage in which the stem cells are located; stem cells are an insufficiently differentiated, yet immature cell with the potential function of regenerating various tissues, organs and human bodies, and are called "universal cells" in the medical community, and are cells with proliferation and differentiation potential, have the ability of self-renewing replication, and can produce highly differentiated functional cells, so that in vitro culture of stem cells under aseptic conditions, research for observing proliferation and growth thereof is particularly important.
At present, an apparatus for accurately and carefully observing the in-vitro culture of stem cells is lacking, and when the in-vitro culture of stem cells is performed, the automated apparatus is fewer, so that certain inconvenience exists in use.
Disclosure of Invention
The invention aims to overcome the existing defects, provides full-automatic stem cell culture equipment, and solves the problems that the equipment for accurately and carefully observing the in-vitro culture of stem cells is lacking in the prior art, and the automatic equipment is less when the in-vitro culture of the stem cells is carried out, and certain inconvenience exists when the automatic equipment is used.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a full automatization stem cell culture equipment, includes equipment outer box, chamber door, cell incubator, business turn over income case, ozone disinfection cabinet, transplanter, dustbin, centrifuge and normal atmospheric temperature test tube storehouse, the inside of equipment outer box is provided with culture platform, the inside one side surface of equipment outer box is provided with the cell incubator, the cell incubator is provided with seven, seven be provided with intelligent microscope between the cell incubator, inside bottom one side of equipment outer box is provided with the transplanter, the lower extreme of transplanter is provided with the dustbin, one side of transplanter is provided with centrifuge, the one end of transplanter is provided with purpose-made culture dish, inside one side upper end sliding connection of equipment outer box has the liquid extractor, one side surface upper end of equipment outer box is provided with normal atmospheric temperature test tube storehouse.
Preferably, a side surface of the equipment outer box is connected with an inlet box in a sliding manner, and a culture liquid medicine is arranged in the inlet box.
Preferably, an ozone disinfection cabinet is arranged at the bottom end of the equipment outer box.
Preferably, a hand grip is arranged on one side surface of the inner part of the equipment outer box through a mechanical structure.
Preferably, a box door is mounted on the side surface of the equipment outer box.
Preferably, an electric cylinder is arranged in the equipment outer box, and the box door is driven to be opened and closed by the electric cylinder.
Preferably, universal wheels are arranged at four corners of the lower end of the equipment outer box, four universal wheels are arranged, and braking structures are arranged on the four universal wheels.
Preferably, the edges of the lower surface of the equipment outer box are fixedly connected with bases, and the bases are provided with six bases.
Compared with the prior art, the invention provides full-automatic stem cell culture equipment, which has the following beneficial effects:
1. according to the invention, through the arranged 7-person cell culture incubator, 7-person cells can be cultured simultaneously through the equipment, so that the cell culture efficiency is greatly accelerated, and through the arranged mechanical equipment such as the grippers and the like, the stem cell culture equipment is more mechanized and intelligent, so that the problem that the existing equipment for accurately and carefully observing the in-vitro culture of stem cells is lacking is effectively avoided, and when the in-vitro culture of stem cells is carried out, the automatic equipment is fewer, and a certain inconvenience exists during the use;
2. the invention realizes the synchronous culture of 7 samples at maximum, the culture period of one sample is 15 days, and the number of cultured cells is 1.0 hundred million;
3. according to the invention, through the arranged inlet and outlet box, when in use, test tubes containing stem cells to be cultured can be placed into the inlet and outlet box through the inlet and outlet box, the test tubes are fed into the culture platform through the inlet and outlet box for culture, when the test tubes are fed into the culture box through the inlet and outlet box for culture, the outer walls of the test tubes containing the stem cells and the like can be subjected to ozone disinfection treatment through the ozone disinfection cabinet, the stem cells can be cultured through the arranged culture liquid medicine, the waste residues in the test tubes can be collected in a concentrated manner through the arranged dustbin, and the problem that the waste residues pollute the culture dishes of the stem cells and cause the failure of stem cell culture is solved;
4. according to the invention, through the intelligent microscope, the states of stem cells cultured in the seven cell culture boxes can be observed in real time in use through the intelligent microscope, the test tubes are conveyed into the cell culture boxes through the grippers to be cultured in use, after the stem cells are cultured, the test tubes can be grasped through the grippers and conveyed into the interior of the inlet/outlet boxes again, the test tubes are conveyed out of the equipment outer boxes through the inlet/outlet boxes, and an operator can directly take out finished test tubes.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate and together with the embodiments of the invention and do not constitute a limitation to the invention, and in which:
FIG. 1 is a schematic diagram of a fully automated stem cell culture apparatus according to the present invention;
FIG. 2 is a schematic diagram of the internal structure of the fully automated stem cell culture apparatus according to the present invention;
in the figure: 1. an equipment outer case; 2. a door; 3. a base; 4. a cell incubator; 5. an intelligent microscope; 6. culturing the liquid medicine; 7. an inlet and outlet box; 8. an ozone disinfection cabinet; 9. a transplanting machine; 10. a dustbin; 11. a centrifuge; 12. specially-made culture dishes; 13. a liquid extractor; 14. a normal temperature test tube bin; 15. a grip.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-2, the present invention provides a technical solution: the invention discloses full-automatic stem cell culture equipment, which comprises an equipment outer box 1, a box door 2, a cell culture box 4, an inlet and outlet box 7, an ozone disinfection cabinet 8, a transplanter 9, a dustbin 10, a centrifugal machine 11 and a normal temperature test tube bin 14, wherein a culture platform is arranged in the equipment outer box 1, the cell culture box 4 is arranged on one side surface of the inside of the equipment outer box 1, seven cell culture boxes 4 are arranged, an intelligent microscope 5 is arranged between the seven cell culture boxes 4, the transplanter 9 is arranged on one side of the inner bottom end of the equipment outer box 1, the dustbin 10 is arranged at the lower end of the transplanter 9, the centrifugal machine 11 is arranged on one side of the transplanter 9, a special culture dish 12 is arranged at one end of the transplanter 9, a liquid extractor 13 is slidingly connected on one side upper end of the equipment outer box 1, the normal temperature test tube bin 14 is arranged on one side surface of the equipment outer box 1, the invention realizes miniaturization and synchronous culture of 7 samples at maximum, has a sample culture period of 15 days, and 1.0 hundred million cultured cells can be put into the interior of the inlet and outlet box 7 through the inlet and outlet box 7 when in use, and test tubes containing the stem cells to be cultured can be put into the interior of the inlet and outlet box 7 through the inlet and outlet box 7 for culture, through the ozone disinfection cabinet 8 that sets up, when carrying out the cultivation in sending into the incubator through business turn over case 7, can carry out ozone disinfection through ozone disinfection cabinet 8 to the test tube outer wall etc. that is equipped with stem cell, through the cultivation liquid medicine 6 of setting, can cultivate the stem cell through cultivateing liquid medicine 6, through the dustbin 10 of setting, can concentrate the collection through the dregs of a meal of dustbin 10 to the test tube inside, prevent that dregs from polluting the culture dish of stem cell, lead to the problem of stem cell culture failure, through the intelligent microscope 5 of setting up, can carry out real-time observation through the state of the stem cell that intelligent microscope 5 can cultivate seven inside cell incubator 4 during the use.
In the present invention, it is preferable that an inlet/outlet tank 7 is slidably connected to one side surface of the equipment enclosure 1, and the inside of the inlet/outlet tank 7 is provided with the culture chemical 6.
In the invention, preferably, the ozone disinfection cabinet 8 is arranged at the bottom end of the inner part of the equipment outer box 1.
In the invention, preferably, a gripper 15 is arranged on one side surface of the interior of the equipment outer box 1 through a mechanical structure, a test tube is sent into the cell culture box 4 through the gripper 15 to be cultured in use, after stem cell culture is completed, the test tube can be grasped through the gripper 15 and is sent into the interior of the access box 7 again, the test tube is sent out of the equipment outer box 1 through the access box 7, and an operator can directly take out a finished test tube.
In the present invention, it is preferable that the side surface of the equipment cabinet 1 is provided with the cabinet door 2.
In the invention, preferably, an electric cylinder is arranged in the equipment outer box 1, and the box door 2 is driven to be opened and closed by the electric cylinder.
In the invention, preferably, universal wheels are arranged at four corners of the lower end of the equipment outer box 1, four universal wheels are arranged, and braking structures are arranged on the four universal wheels.
In the invention, preferably, the bottom surface edge of the equipment outer box 1 is fixedly connected with the base 3, and the base 3 is provided with six.
The working principle and the using flow of the invention are as follows: when in use, an operator puts a collected sample and a culture liquid medicine 6, a test tube and the like used in the culture process into the inlet/outlet box 7, after ozone sterilization and disinfection are carried out in the box, the box door 2 is automatically opened through an electric cylinder, the robot gripper 15 grabs the sample and the test tube to a position appointed by < test tube warehouse > < culture platform >, the robot gripper 15 opens a cover of the sample and the test tube with the culture liquid medicine 6, the reagent transplanting triaxial robot transfers the culture liquid into the sample, the robot shakes the sample test tube uniformly and then puts the sample test tube into the cell culture box 4 for culture, the robot gripper 15 grabs the sample test tube from the cell culture box 4, puts the sample test tube into the centrifuge 11 for centrifugation, the robot gripper 15 takes the sample test tube out, puts the culture platform into the laser scanning test tube liquid layer, and the triaxial transplanting robot wants the test tube to absorb dregs, transferring to a dustbin 10, dispersing essence in the test tubes into 4 test tubes, repeating the previous actions of the 4 test tubes respectively, repeatedly culturing, expanding the quantity of cultured cells, grasping the cells by a robot gripper 15, detecting the cell culturing condition by an intelligent microscope 5, reversely operating the cell quantity to a certain degree, concentrating the cells of the 4 test tubes into 1 finished test tube, grasping the finished test tube by a robot, putting the finished test tube back into an access box 7, automatically closing a box door 2 of the access box 7, taking the finished test tube out by an operator, culturing the cells of 7 persons simultaneously by the aid of the equipment during use, greatly accelerating the efficiency of cell culturing, and enabling a stem cell culturing device to be more mechanical and intelligent by the aid of mechanical equipment such as the gripper 15, the invention effectively avoids the shortage of equipment for accurately and carefully observing the in-vitro culture of stem cells, has fewer automatic equipment when the stem cells are cultured in vitro and certain inconvenience in use, realizes the miniaturization and synchronous culture of 7 samples at maximum, has a sample culture period of 15 days and 1.0 hundred million cultured cells, can put test tubes containing stem cells to be cultured into the inside of the in-out box 7 through the in-out box 7 when in use, can be fed into a culture platform for culture through the in-out box 7, can perform ozone disinfection treatment on the outer walls of test tubes containing the stem cells through the ozone disinfection cabinet 8 when the stem cells are fed into the culture box for culture through the in-out box 7, through the culture liquid medicine 6 that sets up, can cultivate the stem cell through culture liquid medicine 6, through the dustbin 10 that sets up, can concentrate the collection through the dustbin 10 to the inside dregs of a test tube, prevent that dregs from polluting the culture dish of stem cell, lead to the problem that stem cell cultivates failure, through the intelligent microscope 5 that sets up, can carry out real-time observation through the state of seven inside stem cells that cultivates of cell incubator 4 in real time through intelligent microscope 5 during the use, through the tongs 15 that set up, send into the test tube into cell incubator 4 through tongs 15 during the use and cultivate, after the stem cell cultivates, can snatch the test tube through tongs 15, and the inside of reentrant access case 7 is sent out the test tube with the equipment outer case 1 through access case 7, the operator can directly take out finished test tube.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. Full automatization stem cell culture apparatus, including equipment outer box (1), chamber door (2), cell culture case (4), business turn over income case (7), ozone disinfection cabinet (8), transplanter (9), dustbin (10), centrifuge (11) and normal atmospheric temperature test tube storehouse (14), its characterized in that: the inside of equipment outer box (1) is provided with cultivate the platform, the inside one side surface of equipment outer box (1) is provided with cell incubator (4), cell incubator (4) are provided with seven, seven be provided with intelligent microscope (5) between cell incubator (4), inside bottom one side of equipment outer box (1) is provided with transplanter (9), the lower extreme of transplanter (9) is provided with dustbin (10), one side of transplanter (9) is provided with centrifuge (11), one end of transplanter (9) is provided with purpose-built culture dish (12), inside one side upper end sliding connection of equipment outer box (1) has liquid extractor (13), one side surface upper end of equipment outer box (1) is provided with normal atmospheric temperature test tube storehouse (14).
2. The fully automated stem cell culture apparatus of claim 1, wherein: one side surface sliding connection of equipment outer case (1) has business turn over income case (7), the inside of business turn over income case (7) is provided with cultivates liquid medicine (6).
3. The fully automated stem cell culture apparatus of claim 1, wherein: an ozone disinfection cabinet (8) is arranged at the bottom end inside the equipment outer box (1).
4. The fully automated stem cell culture apparatus of claim 1, wherein: the surface of one side of the inner part of the equipment outer box (1) is provided with a grip (15) through a mechanical structure.
5. The fully automated stem cell culture apparatus of claim 1, wherein: the side surface of the equipment outer box (1) is provided with a box door (2).
6. The fully automated stem cell culture apparatus of claim 5, wherein: an electric cylinder is arranged in the equipment outer box (1), and the box door (2) is driven to be opened and closed by the electric cylinder.
7. The fully automated stem cell culture apparatus of claim 1, wherein: the four corners of the lower end of the equipment outer box (1) are provided with universal wheels, the number of the universal wheels is four, and the four universal wheels are provided with braking structures.
8. The fully automated stem cell culture apparatus of claim 1, wherein: the lower surface edge of equipment outer case (1) is all fixedly connected with base (3), base (3) are provided with six.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310393862.4A CN116179352A (en) | 2023-04-13 | 2023-04-13 | Full-automatic stem cell culture equipment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310393862.4A CN116179352A (en) | 2023-04-13 | 2023-04-13 | Full-automatic stem cell culture equipment |
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| Publication Number | Publication Date |
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| CN116179352A true CN116179352A (en) | 2023-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310393862.4A Pending CN116179352A (en) | 2023-04-13 | 2023-04-13 | Full-automatic stem cell culture equipment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
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2023
- 2023-04-13 CN CN202310393862.4A patent/CN116179352A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683634A (en) * | 2024-01-31 | 2024-03-12 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
| CN117683634B (en) * | 2024-01-31 | 2024-05-07 | 山东格林医学科技有限公司 | Stem cell culture device and method for diabetes treatment |
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