CN217868908U - Cell intelligence passage device - Google Patents

Abstract

The utility model relates to a cell intelligence passage device, host computer module (2), passage module (3), storage board module, robot module (5), imaging module (6), input/output module (7), centrifuge module (8) and cultivation module (9) on operation room (1) and operation room (1), storage board module include that the blake bottle stores up board unit (4) and centrifuging tube and store up board unit (11), passage module (3), blake bottle store up board unit (4), robot module (5), imaging module (6) and cultivation module (9) respectively with host computer module (2) electric connection. Compared with the prior art, the utility model has the advantages of degree of automation is high, work efficiency is high, be difficult for receiving pollution, the good reliability and easily augment scale.

Description

Cell intelligence passage device

Technical Field

The utility model belongs to the technical field of the cell culture technique and specifically relates to a cell intelligence passage device is related to.

Background

Mesenchymal stem cells are pluripotent stem cells that have all the commonalities of stem cells, namely self-renewal and multipotent differentiation capacity, and are also the most clinically used. It exists not only in bone marrow but also in skeletal muscle, periosteum and trabecula, and it has a wide range of differentiated tissue types, so it is of great clinical value.

At present, the passage of the mesenchymal stem cells is mostly finished by manual operation, the passage and the culture operation of the manually operated mesenchymal stem cells are influenced by an individual operation instrument, the consistency and the complete normalization of the operation are difficult to ensure, and the operation is easy to pollute. In addition, from the whole process of the subculture and culture work, different actions are clearly divided at different time points, and different time points are provided for when the subculture is carried out, when the culture is carried out, when the imaging is carried out and when the centrifugal action is carried out, but the manual operation cannot be in a working state for 1 day and 24 hours, so the working efficiency is not high.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a cell intelligence passage device in order to overcome the defect that above-mentioned prior art exists, degree of automation is high, work efficiency is high, be difficult for receiving pollution, good reliability, easily increase the scale.

The purpose of the utility model can be realized by the following technical proposal:

the utility model provides a cell intelligence passage device, includes the operation room and the operation room on host computer module, passage module, store up board module, robot module, imaging module, input/output module, centrifuge module and cultivates the module, the storage board module store up board unit and centrifuging tube including the blake bottle and store up the board unit, passage module, blake bottle store up board unit, robot module, imaging module and cultivation module respectively with host computer module electric connection.

Further, the passage module include the movable platform, operating assembly, blake bottle fixing base and the centrifuging tube fixing base in passage module box and the passage module box, operating assembly set up on movable platform, including pipette tip, liquid feeding rifle head, take out waste liquid rifle head, switch cover knob and suction cap valve, passage module box on be equipped with the filter.

Further, the blake bottle store up the board unit and include runing rest, step motor and photoelectric sensor on PMKD and PMKD, the runing rest rotate and set up on PMKD, and be connected with the step motor transmission, the runing rest on use its axis to be equipped with a plurality of as central symmetry and be used for depositing the first storage board support of blake bottle, photoelectric sensor towards the axis of runing rest.

Furthermore, the robot module comprises a robot base, a mechanical arm and a mechanical arm clamping jaw which are connected in sequence.

Further, the imaging module comprises an imaging module box body and a microscope of the imaging module box body, wherein the imaging module box body is provided with a small access door, a transmission interface and a movable sampling platform for moving the culture bottle (10), and the transmission interface is electrically connected with the microscope.

Furthermore, the input/output module comprises an input/output module box body and a storage rack in the input/output module box body, and the input/output module box body is arranged on the operation room.

Further, the centrifuge module comprises a centrifuge module box body and a centrifuge in the centrifuge module box body, a switch and an operation screen are arranged on the centrifuge module box body, and the switch and the operation screen are respectively electrically connected with the centrifuge.

Further, the cultivation module comprises an incubator, and an opening and closing door is arranged on the incubator.

Furthermore, centrifuging tube store up board unit include that the second stores up the fixed plate of board support and being used for fixed centrifuging tube, the second store up the board support and fix on the operation room lateral wall, the fixed plate locate the second store up on the board support, be equipped with the handle on the centrifuging tube.

Furthermore, the upper computer module comprises an upper computer and a keyboard electrically connected with the upper computer, and the upper computer and the keyboard are arranged on the side wall of the operating room.

Compared with the prior art, the utility model discloses have with following beneficial effect:

(1) The utility model discloses cell intelligence passage device has passage concurrently, cultivate, multi-functional in an organic whole such as formation of image and centrifugation, all realize unmanned operation from processes such as seeding, cultivation, formation of image and centrifugation of cell, cell intelligence passage device control whole process flow operation, the condition of monitoring every action completion realizes the complete automation of cell preparation operation, incessant execution action, work efficiency is high, use manpower sparingly, the whole flow of cultivateing simultaneously need not artificial intervention, thereby avoided the biggest pollution source, make the final product difficult to receive the pollution;

(2) The utility model discloses functions such as passage, cultivation, formation of image and centrifugation can the autonomous working, are not influenced each other, and the conversion is free, realizes cell preparation operation modularization, and the existence and the quantity of all functional modules can freely arrange according to specific use scene and required productivity. For example, the industrial scale can be simply and conveniently expanded by increasing the number of culture modules and plate storage modules;

(3) The cell intelligent passage device of the utility model can completely realize the consistency of the action by depending on a digital system, and the action degree and the action standard requirement can be kept completely consistent, thereby overcoming the uncertainty caused by manual operation, having strong reliability and improving the standardization level of the whole cell preparation process;

(4) The cell intelligent passage device of the utility model is suitable for culture containers of different types, comprises culture bottles, culture dishes and perforated plates of various specifications, and has wide application range;

(5) The utility model discloses the passage module includes passage module box and the mobile platform in the passage module box, operating module, blake bottle fixing base and centrifuging tube fixing base, and operating module sets up on mobile platform, including liquid-transfering gun head, liquid-adding gun head, take out waste liquid rifle head, switch lid knob and suction cap valve, passage module passes through mobile platform's removal independent assortment operating module, gathers blake bottle and centrifuging tube suction cap, put the operation of covering and the liquid-adding of multiple liquid, take out waste liquid and move the liquid work between different utensils in an organic whole, realizes full-automatic seeding passage operation;

(6) The utility model discloses an imaging module automatic acquisition cell image to cell morphology analysis and cell count are carried out through corresponding algorithm is automatic, thereby generate cell proliferation curve as the feedback, and the intelligent growth state of judging current cell, thereby the decision system carries out next step corresponding action (if carry out the passage or trade the liquid operation), realizes closed-loop control, is favorable to process monitoring and to the control of result, and the good reliability can observe the form of cell growth simultaneously;

(7) The utility model discloses it is rotatory that the blake bottle stores up the step motor control runing rest in the board unit, through the position of photoelectric sensor feedback runing rest, finally makes required storage board support towards robot module, and the robot module of being convenient for deposits and takes out the blake bottle.

Drawings

FIG. 1 is a schematic front perspective view of an operating room;

FIG. 2 is a schematic side perspective view of an operating room;

FIG. 3 is a schematic view of a rear perspective structure of the operating room; (ii) a

FIG. 4 is a front view of an operator compartment;

FIG. 5 is a schematic perspective view of a passaging module;

FIG. 6 is a front view of a passaging module;

FIG. 7 is a schematic perspective view of a plate storage unit of a culture bottle;

FIG. 8 is a schematic perspective view of a robot module;

FIG. 9 is a schematic perspective view of an imaging module;

fig. 10 is a schematic perspective view of an input/output module;

FIG. 11 is a schematic perspective view of a centrifuge module;

FIG. 12 is a schematic perspective view of the culture module;

FIG. 13 is a schematic perspective view of a centrifuge tube plate storage unit;

the reference numbers in the figures illustrate:

1. the system comprises an operating room, 2, an upper computer module, 3, a passage module, 4, a culture bottle storage plate unit, 5, a robot module, 6, an imaging module, 7, an input and output module, 8, a centrifuge module, 9, a culture module, 10, a culture bottle, 11, a centrifuge tube storage plate unit, 12, a centrifuge tube, 31, a passage module box body, 21, an upper computer, 22, a keyboard, 32, a filter, 33, a pipette tip, 34, a liquid feeding pipette tip, 35, a moving platform, 36, a culture bottle fixing seat, 37, 38, a waste liquid pumping pipette tip, 39, a switch cover knob, 40, a suction cover valve, 41, a fixing bottom plate, 42, a rotating support, 43, a first storage plate support, 44, a stepping motor, 45, a photoelectric sensor, 51, a robot base, 52, a mechanical arm, 53, a mechanical arm clamping jaw, 61, an imaging module box body, 62, an access small door, 63, a transmission interface, 71, 72, a storage frame, 81, a centrifuge module box body, 82, a switch box body, 83, an operating switch box, a 92, a handle, a second storage plate and a centrifuge tube fixing plate.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and specific embodiments. The embodiment of the present invention is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the scope of the present invention is not limited to the following embodiments.

The utility model provides a cell intelligence passage device, if fig. 1, fig. 2, fig. 3 and fig. 4, including the operation room 1 and the operation room 1 on host computer module 2, passage module 3, store up the board module, robot module 5, imaging module 6, input/output module 7, centrifuge module 8 and cultivation module 9, store up the board module and store up board unit 4 and centrifuging tube including the blake bottle and store up board unit 11, passage module 3, blake bottle store up board unit 4, robot module 5, imaging module 6 and cultivation module 9 respectively with host computer module 2 electric connection.

As shown in fig. 1, the upper computer module 2 includes an upper computer 21 and a keyboard 22 electrically connected to the upper computer 21, and the upper computer 21 and the keyboard 22 are disposed on a side wall of the operating room 1. The upper computer 21 is a set of PC device, a set of application for controlling the cell intelligent passage system is pre-installed, and the application comprises all possible general instructions (the general instructions are instructions sent to the passage module 3, the culture bottle storage plate unit 4, the robot module 5, the imaging module 6 and the culture module 9 by the upper computer 21, such as a culture bottle uncovering instruction of the passage module 3, a culture bottle taking instruction of the culture bottle storage plate unit 4 or a culture bottle putting instruction of the culture module 9, and the like, the user selects and combines different general instructions to form a specific process instruction according to different process requirements, the system can be operated after reasonable parameters are set, and meanwhile, the instruction operation condition can be detected.

The operation interface of the upper computer 21 is free independently, and an operator can independently select different instructions and different parameter settings according to different process operation requirements to realize differentiated process instruction requirements.

Passage module 3 is the core of cell intelligence passage device, mainly can open, close the lid operation to centrifuging tube 12, blake bottle 10, simultaneously according to the technology needs, carries out the liquid feeding in different technology sections, moves the liquid, takes out waste liquid operation etc.. As shown in fig. 5 and 6, the passaging module 3 includes a passaging module box 31, a moving platform 35, an operating assembly, a culture bottle fixing seat 36 and a centrifuge tube fixing seat 37, the moving platform 35, the operating assembly, the culture bottle fixing seat 36 and the centrifuge tube fixing seat 37 are arranged in the passaging module box 31, the operating assembly is arranged on the moving platform 35 and includes a pipette tip 33, a liquid adding tip 34, a waste liquid pumping tip 38, a switch cover knob 39 and a suction cover valve 40, the culture bottle fixing seat 36 is used for fixing the culture bottle 10, the centrifuge tube fixing seat 37 is used for fixing the centrifuge tube 12, and the passaging module box 31 is provided with a filter 32 to ensure that the inside of the passaging module box 31 is not polluted;

the liquid feeding of passage module 3, take out the waste liquid, move the liquid and can the independent assortment use through the removal of moving platform 35, the liquid feeding of the multiple liquid of set, take out the waste liquid and different utensil room move liquid work in an organic whole, can carry out centrifuging tube 12 and blake bottle 10 the installation, lift off, inhale the lid and put the lid operation, realize full-automatic seeding passage operation.

The blake bottle stores up board unit 4 and is used for storing blake bottle 10, and centrifuging tube stores up board unit 11 and is used for storing centrifuging tube 12, and blake bottle 10 and centrifuging tube 12 use as the annex in cell intelligence passage device, and when the seeding stage, new blake bottle 10 is as annex input cell intelligence passage device, and when the centrifugation stage, new centrifuging tube 12 is as annex input cell intelligence passage device.

As shown in fig. 7, the culture bottle plate storage unit 4 comprises a fixed bottom plate 41, and a rotating bracket 42, a stepping motor 44 and a photoelectric sensor 45 which are arranged on the fixed bottom plate 41, wherein the rotating bracket 42 is rotatably arranged on the fixed bottom plate 41 and is in transmission connection with the stepping motor 44, a plurality of first plate storage brackets 43 for storing the culture bottles 10 are symmetrically arranged on the rotating bracket 42 by taking the axis thereof as the center, and the photoelectric sensor 45 faces the axis of the rotating bracket 42;

the stepping motor 44 controls the rotation of the rotary bracket 42, and the position of the rotary bracket 42 is fed back through the photoelectric sensor 45, so that the required first plate storage bracket 43 is finally oriented to the robot module 5, and the robot module 5 can conveniently store and take out the culture bottles 10.

As shown in fig. 13, the centrifuge tube plate storage unit 11 includes a second plate storage rack 111 and a fixing plate 112 for fixing the centrifuge tube 12, the second plate storage rack 111 is fixed on the sidewall of the operating room 1, the fixing plate 112 is disposed on the second plate storage rack 111, and the centrifuge tube is provided with a handle 113;

the centrifuged centrifuge tube 12 extracts waste liquid in the passage module 3, then adds culture medium, then snatchs through the robot module 5, puts on the centrifuge tube storage plate unit 11 as a cell after passage, and waits for the next step of operation.

As shown in fig. 8, the robot module 5 includes a robot base 51, a robot arm 52, and a robot arm gripper 53 connected in this order.

As shown in fig. 9, the imaging module 6 includes an imaging module case 61 and a microscope of the imaging module case 61, the imaging module case 61 is provided with an access door 62, a transmission interface 63 and a mobile sampling platform for moving the culture bottle 10, and the transmission interface 63 is electrically connected with the microscope;

the culture bottle 10 filled with the cells in the culture stage is placed in the imaging module box body 61 according to the needs, the position of the culture bottle 10 can be adjusted, any position of the cells in the culture bottle 10 is focused and photographed, the number of the cells at the point is automatically recorded according to the cell characteristics after an image is generated, the cell quantity popularized in the whole culture bottle 10 is the reference and basis for judging the cell passage time, the cell growth condition is judged through cell counting, corresponding operations are executed according to the density and saturation of the cells at different moments, and the functions of cell culture and passage are realized.

As shown in fig. 10, the input/output module 7 includes an input/output module case 71 and a storage rack 72 in the input/output module case 71, wherein the input/output module case 71 is provided on the operation room 1;

cell intelligence passage device puts into culture bottle 10 to the cell stock solution after resumeing before the work, places culture bottle 10 on the storage frame 72 in input/output module box 71 again, makes things convenient for robot module 5 to take and puts into passage module 3 and sow.

As shown in fig. 11, the centrifuge module 8 includes a centrifuge module box 81 and a centrifuge in the centrifuge module box 81, the centrifuge module box 81 is provided with a switch 82 and an operation panel 83, and the switch 82 and the operation panel 83 are respectively electrically connected to the centrifuge;

in the passage module 3, the cultured cells are transferred to the centrifuge tube 12 from the culture bottle 10, the centrifuge tube 12 is placed into the centrifuge, the robot module 5 sets the rotating speed and time through the operation screen 83, the switch 82 controls the start of the centrifuge to perform centrifugal work, the centrifugal aim is to further separate the cells from the cell sap, and the centrifuge tube 12 is placed into the passage module 3 after the centrifugation is finished.

As shown in fig. 12, the cultivation module 9 includes an cultivation box 91, and an opening/closing door 92 is provided on the cultivation box 91;

the cell stock solution after seeding is added with the culture medium and then placed in the culture flask 10, the culture shelf 94 is used for placing the culture flask 10, the incubator 91 maintains the environment of 37 ℃ of temperature, 90% RH or more of humidity and 5% of carbon dioxide, and the cell culture work is carried out, during which the work of extracting waste liquid and adding the culture medium to the culture flask 10 containing the cell stock solution is possible according to the growth of the cells;

when the flask 10 is to be stored, the opening and closing door 92 is opened, and the robot module 5 grips the flask 10 by the arm gripper 53 and places the flask into the incubator 91.

The working process of the cell intelligent passage device comprises the following steps:

s1, during passage, the robot module 5 places a culture bottle 10 in an incubator 91 into a passage module 3, waste liquid extraction is carried out on cell sap in the culture bottle 10, then digestive enzyme is added and the cell sap is shaken up, the robot module 5 places the culture bottle 10 back into the incubator 91 for incubation for a plurality of minutes, after the time comes, the robot module 5 takes out the culture bottle 10 from the culture box 10, places the culture bottle 10 into the passage module 3, and adds culture medium to stop digestion;

s2, the robot module 5 takes a new centrifuge tube 12 from the centrifuge tube storage plate unit 11 and puts the centrifuge tube 12 into the passage module 3, the cell sap in the culture bottle 10 processed in the step S1 is transferred into the centrifuge tube 12 through the pipette tip 33, meanwhile PBS is added into the culture bottle 10, the centrifuge tube is cleaned and shaken evenly, then the pipette is moved into the centrifuge tube 12, after the pipette action is completed, the robot discards the culture bottle 10, then the centrifuge tube 12 is put into a centrifuge, after the rotation speed and time are set, the centrifuge starts to work, after the preset time is reached, the centrifuge stops working, the robot module 5 takes out the centrifuge tube 12 and puts into the passage machine, waste liquid is pumped out, culture medium is added to form cells of P1 generation, and then the actions of seeding, culturing, imaging, centrifuging and the like are repeated according to the process requirements, so that the cells of P2 generation of cells of P1 generation and the cells of P3 generation of P2 generation are passaged, and the passage is repeated.

When the culture bottle 10 and the centrifuge tube 12 enter the passage module 3 through the robot module 5, the bottle caps are screwed down, and when liquid adding, waste liquid pumping and liquid transferring operations are required to be performed on the centrifuge tube 12 or the culture bottle 10, the bottle caps are unscrewed and then screwed up before the culture bottle is moved out of the passage module 3; before the waste liquid pumping action, a special Tip head needs to be additionally arranged, and the Tip head is removed after the action is finished; before the liquid transferring action, a liquid transferring pipe needs to be additionally arranged, and the liquid transferring pipe is removed after the liquid transferring work is finished.

This embodiment provides a cell intelligence passage device, have the passage concurrently, cultivate, multi-functional in an organic whole such as formation of image and centrifugation, realize cell preparation operation systematization, the passage, cultivate, functions such as formation of image and centrifugation can work alone, not influenced each other, the conversion is free, realize cell preparation operation modularization, passage module 3's liquid feeding, take out the waste liquid, move the liquid and can the independent assortment use through the removal of moving platform 35, can cultivate the quantity of module 9 and storage plate module through increasing, realize the amplification industrial scale simply conveniently, produce the cell proliferation curve as the feedback through imaging module 6, intelligent judgement system carries out corresponding action on next step.

The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the teachings of the present invention without undue experimentation. Therefore, the technical solutions that can be obtained by logical analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention by those skilled in the art should be within the scope of protection defined by the claims.

Claims (10)

1. The utility model provides a cell intelligence passage device, its characterized in that, host computer module (2), passage module (3), storage board module, robot module (5), imaging module (6), input/output module (7), centrifuge module (8) and cultivation module (9) on (1) between (1) and operation including the operation, storage board module include that the blake bottle stores up board unit (4) and centrifuging tube and stores up board unit (11), passage module (3), blake bottle store up board unit (4), robot module (5), imaging module (6) and cultivation module (9) respectively with host computer module (2) electric connection.

2. The intelligent cell passage device according to claim 1, wherein the passage module (3) comprises a passage module box body (31), and a moving platform (35), an operating assembly, a culture bottle fixing seat (36) and a centrifuge tube fixing seat (37) which are arranged in the passage module box body (31), the operating assembly is arranged on the moving platform (35) and comprises a liquid transfer gun head (33), a liquid transfer gun head (34), a waste liquid pumping gun head (38), a switch cover knob (39) and a suction cover valve (40), and a filter (32) is arranged on the passage module box body (31).

3. The intelligent cell passaging device of claim 1, wherein the culture bottle plate storage unit (4) comprises a fixed bottom plate (41), a rotating support (42) on the fixed bottom plate (41), a stepping motor (44) and a photoelectric sensor (45), the rotating support (42) is rotatably arranged on the fixed bottom plate (41) and is in transmission connection with the stepping motor (44), the rotating support (42) is symmetrically provided with a plurality of first plate storage supports (43) for storing culture bottles (10) by taking the axis of the rotating support as a center, and the photoelectric sensor (45) faces the axis of the rotating support (42).

4. The intelligent cell passaging device of claim 1, wherein the robot module (5) comprises a robot base (51), a mechanical arm (52) and a mechanical arm clamping jaw (53) which are connected in sequence.

5. The intelligent cell passaging device according to claim 1, wherein the imaging module (6) comprises an imaging module box (61) and a microscope of the imaging module box (61), the imaging module box (61) is provided with an access door (62), a transmission interface (63) and a movable sampling platform for moving the culture flask (10), and the transmission interface (63) is electrically connected with the microscope.

6. The intelligent cell passaging device of claim 1, wherein the input/output module (7) comprises an input/output module box (71) and a storage rack (72) in the input/output module box (71), and the input/output module box (71) is arranged on the operation room (1).

7. The intelligent cell passaging device of claim 1, wherein the centrifuge module (8) comprises a centrifuge module box (81) and a centrifuge in the centrifuge module box (81), the centrifuge module box (81) is provided with a switch (82) and an operation screen (83), and the switch (82) and the operation screen (83) are respectively electrically connected with the centrifuge.

8. The intelligent cell passaging device according to claim 1, wherein the culturing module (9) comprises an incubator (91), and an opening and closing door (92) is arranged on the incubator (91).

9. The intelligent cell generation device of claim 1, wherein the centrifuge tube plate storage unit (11) comprises a second plate storage bracket (111) and a fixing plate (112) for fixing the centrifuge tube (12), the second plate storage bracket (111) is fixed on the side wall of the operation room (1), the fixing plate (112) is arranged on the second plate storage bracket (111), and the centrifuge tube is provided with a handle (113).

10. The intelligent cell generation device according to claim 1, wherein the upper computer module (2) comprises an upper computer (21) and a keyboard (22) electrically connected with the upper computer (21), and the upper computer (21) and the keyboard (22) are arranged on the side wall of the operating room (1).

Images