CN216073842U

CN216073842U - Full-automatic lentivirus infection equipment

Info

Publication number: CN216073842U
Application number: CN202121291773.1U
Authority: CN
Inventors: 肖腾飞
Original assignee: Shanghai Xunbaihui Biotechnology Co ltd
Current assignee: Shanghai Xunbaihui Biotechnology Co ltd
Priority date: 2021-06-10
Filing date: 2021-06-10
Publication date: 2022-03-18
Anticipated expiration: 2031-06-10

Abstract

The utility model relates to the technical field of lentivirus infection, in particular to a full-automatic lentivirus infection device, which comprises a box body, wherein one side of the box body is hinged with a box door, the inner side of the box door is provided with a containing cavity, the bottom of the containing cavity is provided with a shaking table, the top of the shaking table is fixedly provided with a culture plate, the culture plate is provided with a plurality of containing grooves for containing culture solution, the culture plate is detachably provided with a cover plate, the top of the containing cavity is provided with an operating mechanism, the operating mechanism comprises a taking component and a sample adding component which are arranged in parallel, the top of the containing cavity is also provided with a driving mechanism for driving the operating mechanism to move on a horizontal plane, and the full-automatic lentivirus infection device can automatically infect cells so as to ensure the infection efficiency and improve the success rate of experiments.

Description

Full-automatic lentivirus infection equipment

Technical Field

The utility model relates to the technical field of lentivirus infection, in particular to a full-automatic lentivirus infection device.

Background

Lentivirus (Lentivirus) vectors are gene therapy vectors developed based on HIV-1 (human immunodeficiency virus type I). A distinction is made between retroviral vectors in general, which have the ability to infect both dividing and non-dividing cells.

The vector can effectively integrate the exogenous gene onto the host chromosome, thereby achieving persistent expression. Can effectively infect various types of cells such as neuron cells, liver cells, myocardial cells, tumor cells, endothelial cells, stem cells and the like in the aspect of infection capacity, thereby achieving good gene therapy effect, developing clinical research in the United states, having ideal effect and having wide application prospect.

At present, when the lentivirus infectious cells are used, the infection is generally carried out manually, the operation steps are simple but the infection amount is large, the experiment is long in time consumption and easy to operate by mistake, and once the experiment fails due to the operation by mistake, the repeated operation is needed, so that more time and expenditure are needed.

SUMMERY OF THE UTILITY MODEL

In order to solve the above technical problems, the present invention provides a fully automatic lentivirus infection apparatus, which can automatically infect cells to ensure infection efficiency and improve success rate of experiments.

In order to achieve the technical effect, the utility model adopts the following technical scheme:

a full-automatic lentivirus infection device comprises a box body, wherein one side of the box body is hinged with a box door, the inner side of the box door is provided with a containing cavity, the bottom of the containing cavity is provided with a shaking table, the top of the shaking table is fixedly provided with a culture plate, the culture plate is provided with a plurality of containing grooves for containing culture solution, the culture plate is detachably provided with an cover plate, the top of the containing cavity is provided with an operating mechanism, the operating mechanism comprises a taking component and an adding component which are arranged in parallel, the taking component is used for taking and placing the cover plate, the adding component is used for adding reagent into the containing grooves, the adding component at least comprises an injecting needle head, the injecting end of the injecting needle head is provided with an injecting tube, the top of the containing cavity is also provided with a driving mechanism for driving the operating mechanism to move on the horizontal plane, and a controller is also arranged in the box body, the driving mechanism and the operating mechanism are electrically connected with the controller.

Further, one side of the box body is at least provided with a mounting groove, a sealing plate is arranged at the notch of the mounting groove, the sealing plate is in sliding connection with the box body, an exchangeable reagent bottle is arranged in the mounting groove, and the sample introduction end of the liquid injection pipe is arranged in the mounting groove and is inserted in the reagent bottle.

Further, the top of mounting groove is equipped with the ultraviolet lamp, the ultraviolet lamp with the controller electricity is connected.

Further, operating device includes the mount pad, the subassembly of taking is including locating the first connecting plate of mount pad below, the bottom of first connecting plate is equipped with a vacuum chuck at least, first connecting plate through first cylinder with mount pad fixed connection.

Further, actuating mechanism is including locating X axle sharp module and the sharp module of Y axle at holding chamber top, X axle sharp module and the sharp module of Y axle all with the controller electricity is connected.

Further, the application of sample subassembly still with mount pad fixed connection's second connecting plate, the bottom of second connecting plate is rotated through the pivot and is connected with the mounting panel, annotate the liquid syringe needle interval and set up uniformly just be circumference form distribution on the mounting panel, the below of second connecting plate is equipped with the protection casing, just one has been seted up to the bottom of protection casing and has been annotated the liquid through-hole, annotate the position of liquid through-hole with it is corresponding to annotate the position of liquid syringe needle, just still be equipped with on the second connecting plate and be used for the drive mounting panel pivoted drive assembly.

Further, the driving assembly comprises a driving motor, a first bevel gear is arranged at the output end of the driving motor, a second bevel gear is arranged outside the rotating shaft, and the second bevel gear is meshed with the first bevel gear.

Further, a second air cylinder is arranged between the second connecting plate and the mounting seat.

Compared with the prior art, the utility model has the beneficial effects that: according to the full-automatic lentivirus infection equipment provided by the utility model, the accommodating cavity is arranged in the box body, the culture plate is arranged in the accommodating cavity like a shaking table, and the driving mechanism is arranged right above the culture plate and used for driving the operating mechanism to pick and place the cover plate on the culture plate and automatically sample injection, so that the automatic infection process of lentivirus on cells is realized. The process can replace manual work, has high operation efficiency, can effectively reduce errors in the manual operation process, and improves the success rate of cell infection.

Drawings

FIG. 1 is a schematic diagram of an overall structure of a fully automatic lentivirus infection apparatus according to an embodiment of the present invention;

FIG. 2 is an enlarged partial schematic view of a fully automatic lentivirus infection apparatus at A according to an embodiment of the present invention;

the reference signs are: 10, a box body, 11, a containing cavity, 20, a shaking table, 21, a caulking groove, 30, a culture plate, 31, a containing groove, 32, a cover plate, 40, a controller, 50, a mounting groove, 51, a sealing plate, 52, a reagent bottle, 53, a liquid injection pipe, 54, a peristaltic pump, 55, an ultraviolet lamp, 60, an X-axis linear module, 61, a Y-axis linear module, 70, a mounting seat, 80, a first connecting plate, 81, a vacuum chuck, 82, a first cylinder, 90, a second connecting plate, 91, a second cylinder, 92, a rotating shaft, 93, a mounting plate, 931, a liquid injection needle, 94, a protective cover, 941, a liquid injection through hole, 95, a driving motor, 951, a first bevel gear, 952, and a second bevel gear.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

As shown in fig. 1-2, the fully automatic lentivirus infection apparatus provided in this embodiment includes a box body 10, a box door is hinged to one side of the box body 10, an accommodating chamber 11 is disposed on an inner side of the box door, a shaking table 20 is disposed at a bottom of the accommodating chamber 11, and a shaking table assembly is disposed at a bottom of the shaking table 20 for driving the shaking table 20 to shake so as to uniformly mix liquid in a culture plate 30. The rocking platform assembly is prior art and therefore, will not be described further herein. The top of the shaking table 20 is fixedly provided with a culture plate 30, the top of the shaking table 20 is provided with an embedding groove 21 or a magnetic suction component, and the bottom of the culture plate 30 is embedded in the embedding groove 21 or fixed with the shaking table 20 through magnetic suction. The culture plate 30 is provided with a plurality of accommodating grooves 31 for accommodating culture solution, a cover plate 32 is detachably mounted on the culture plate 30, and specifically, the cover plate 32 covers the culture plate 30. The top of holding chamber 11 is equipped with operating device, operating device is including the subassembly and the application of sample subassembly of taking of parallel arrangement, the subassembly of taking is used for getting apron 32 and puts, the application of sample subassembly is arranged in adding reagent to storage tank 31, the application of sample subassembly includes that a plurality of annotates liquid syringe 931 just the introduction end of annotating liquid syringe 931 is equipped with annotates liquid pipe 53, the top of holding chamber 11 still is equipped with and is used for the drive actuating mechanism that operating device removed on the horizontal plane, still be equipped with controller 40 in the box 10, actuating mechanism, operating device all with controller 40 electricity is connected. When the cell culture device is specifically implemented, the controller 40 controls the driving assembly to drive the operating mechanism to sequentially carry out cover taking, sample adding and cover adding operations on the culture plate 30, and then the shaking table assembly is matched with the liquid in the accommodating groove 31 to be uniformly mixed, so that cells are incubated. When a long-term incubation is required, the plate 30 and the cover may be taken out together and placed in an incubator for cultivation. The full-automatic lentivirus infection equipment can replace manual work to continuously and automatically sample the cell culture plate 30, and can effectively avoid errors caused by manual operation.

In this embodiment, a plurality of mounting grooves 50 are formed in one side of the case 10, and the number of the mounting grooves 50 corresponds to the number of the injection needles 931. The reagent bottle testing device is characterized in that a sealing plate 51 is arranged at the notch of the mounting groove 50, the sealing plate 51 is in sliding connection with the box body 10, an exchangeable reagent bottle 52 is arranged in the mounting groove 50, and a sample introduction end of a liquid injection pipe 53 is arranged in the mounting groove 50 and is inserted into the reagent bottle 52. Specifically, a peristaltic pump 54 is disposed on the liquid injection pipe 53, and the peristaltic pump 54 is electrically connected to the controller 40. In specific implementation, the sealing plate 51 can be slid to facilitate taking and placing the reagent bottle 52, the sample injection end of the injection tube 53 is inserted into the bottom of the reagent bottle 52, and the controller 40 controls the peristaltic pump 54 to operate, so as to pump the reagent to be added in the reagent bottle 52 into the injection tube 53 and flow out through the injection needle 931, so as to add the sample into the accommodating groove 31. Meanwhile, in order to avoid the mixed bacteria, an ultraviolet lamp 55 is arranged at the top of the installation groove 50, the ultraviolet lamp 55 is electrically connected with the controller 40, and the controller 40 can control the ultraviolet lamp 55 to sterilize the installation groove 50 during the specific implementation.

In this embodiment, the driving mechanism includes an X-axis linear module 60 and a Y-axis linear module 61 disposed at the top of the accommodating chamber 11, and both the X-axis linear module 60 and the Y-axis linear module 61 are electrically connected to the controller 40. In specific implementation, the controller 40 controls the X-axis linear module 60 and the Y-axis linear module 61 to cooperate with each other to realize the alignment of the liquid injection needle 931 and the accommodating groove 31, so as to sequentially sample the accommodating groove 31.

In this embodiment, the operating mechanism includes a mounting seat 70, the taking assembly includes a first connecting plate 80 disposed below the mounting seat 70, a plurality of vacuum suction cups 81 are disposed at the bottom of the first connecting plate 80, and the first connecting plate 80 is fixedly connected to the mounting seat 70 through a first air cylinder 82. During specific implementation, the controller 40 controls the driving mechanism to drive the first connecting plate 80 to move to the position right above the culture plate 30, and then the controller controls the first cylinder 82 to extend so that the vacuum chuck 81 can take the cover body, thereby facilitating the subsequent sample adding operation.

In this embodiment, the application of sample subassembly still with mount pad 70 fixed connection's second connecting plate 90, the bottom of second connecting plate 90 is rotated through pivot 92 and is connected with mounting panel 93, it sets up evenly to annotate liquid syringe needle 931 interval on the mounting panel 93 and be the circumference form and distribute, the below of second connecting plate 90 is equipped with protection casing 94, just one notes liquid through-hole 941 has been seted up to the bottom of protection casing 94, the position of annotating liquid through-hole 941 with it is corresponding to annotate the position of liquid syringe needle 931, just still be equipped with on the second connecting plate 90 and be used for the drive mounting panel 93 pivoted drive assembly. Specifically, the driving assembly includes a driving motor 95, a first bevel gear 951 is disposed at an output end of the driving motor 95, and the driving motor 95 can drive the first bevel gear 951 to rotate forward or backward. A second bevel gear 952 is disposed outside the rotating shaft 92, and the second bevel gear 952 is engaged with the first bevel gear 951. In addition, a second cylinder 91 is disposed between the second connecting plate 90 and the mounting seat 70. The driving motor 95 and the second cylinder 91 are both electrically connected to the controller 40. When the implementation is specific, by controller 40 control drive assembly drives pivot 92 and rotates to make different notes liquid syringe needle 931 correspond with notes liquid through-hole 941, at this moment, by controller 40 control corresponds peristaltic pump 54 work, so that add the reagent that corresponds to storage tank 31 in, realize automated operation.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the utility model as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims

1. A full-automatic lentivirus infection equipment which is characterized in that: including box (10), one side of box (10) articulates there is the chamber door, just the inboard of chamber door is equipped with holding chamber (11), holding chamber (11) bottom is equipped with shaking table (20), the top fixed mounting who shakes shaking table (20) has culture plate (30), be equipped with on culture plate (30) a plurality of storage tanks (31) and be used for holding the culture solution, detachably is equipped with apron (32) on culture plate (30), the top of holding chamber (11) is equipped with operating device, operating device is including the subassembly and the application of sample subassembly of taking of parallel arrangement, the subassembly of taking is used for getting apron (32) and puts, the application of sample subassembly is arranged in adding reagent to storage tank (31), the application of sample subassembly includes that one annotates liquid syringe needle (931) just at least the appearance end of annotating liquid syringe needle (931) is equipped with annotates liquid pipe (53), the top of holding chamber (11) still is equipped with and is used for the drive operating device moves on the horizontal plane The structure, still be equipped with controller (40) in box (10), actuating mechanism, operating device all with controller (40) electricity is connected.

2. A fully automated lentivirus infection apparatus according to claim 1, wherein: one side of the box body (10) is at least provided with one mounting groove (50), a sealing plate (51) is arranged at the notch of the mounting groove (50), the sealing plate (51) is in sliding connection with the box body (10), an exchangeable reagent bottle (52) is arranged in the mounting groove (50), and the sample injection end of the liquid injection pipe (53) is arranged in the mounting groove (50) and is inserted in the reagent bottle (52).

3. A fully automated lentivirus infection apparatus according to claim 2, wherein: an ultraviolet lamp (55) is arranged at the top of the mounting groove (50), and the ultraviolet lamp (55) is electrically connected with the controller (40).

4. A fully automated lentivirus infection apparatus according to claim 1, wherein: the operating mechanism comprises a mounting seat (70), the taking assembly comprises a first connecting plate (80) arranged below the mounting seat (70), at least one vacuum suction cup (81) is arranged at the bottom of the first connecting plate (80), and the first connecting plate (80) is fixedly connected with the mounting seat (70) through a first air cylinder (82).

5. A fully automated lentivirus infection apparatus according to claim 1, wherein: the driving mechanism comprises an X-axis linear module (60) and a Y-axis linear module (61) which are arranged at the top of the accommodating cavity (11), and the X-axis linear module (60) and the Y-axis linear module (61) are electrically connected with the controller (40).

6. The fully automated lentivirus infection apparatus of claim 4, wherein: the application of sample subassembly still with mount pad (70) fixed connection's second connecting plate (90), the bottom of second connecting plate (90) is rotated through pivot (92) and is connected with mounting panel (93), it sets up evenly to annotate liquid syringe needle (931) interval on mounting panel (93) and be the circumference form and distribute, the below of second connecting plate (90) is equipped with protection casing (94), just one notes liquid through-hole (941) has been seted up to the bottom of protection casing (94), annotate the position of liquid through-hole (941) with the position of annotating liquid syringe needle (931) is corresponding, just still be equipped with on second connecting plate (90) and be used for the drive mounting panel (93) pivoted drive assembly.

7. The fully automated lentivirus infection apparatus of claim 6, further comprising: the driving assembly comprises a driving motor (95), a first bevel gear (951) is arranged at the output end of the driving motor (95), a second bevel gear (952) is arranged outside the rotating shaft (92), and the second bevel gear (952) is meshed with the first bevel gear (951).

8. The fully automated lentivirus infection apparatus of claim 6, further comprising: and a second air cylinder (91) is also arranged between the second connecting plate (90) and the mounting seat (70).