CN219495858U - Chromosome preparation device - Google Patents

Abstract

The utility model discloses a chromosome preparation device which comprises a sample adding cabinet, a culture cabinet, a harvesting cabinet, a dripping baking cabinet, a dyeing piece cabinet and a conveying track, wherein the sample adding cabinet is connected with the culture cabinet; the sample adding cabinet is provided with a sample inlet, the sample dyeing cabinet is provided with a sample outlet, one end of the conveying rail is arranged in the sample adding cabinet and positioned at the sample inlet, and the other end of the conveying rail sequentially penetrates through the culture cabinet, the harvesting cabinet, the drip-baking cabinet and the sample dyeing cabinet and penetrates out of the sample dyeing cabinet from the sample outlet; a first sample adding mechanism, a first clamping mechanism, a first reagent box, a first water bath box and a first operation table are arranged in the sample adding cabinet; the harvesting cabinet is internally provided with a second sample adding mechanism, a second clamping mechanism, a second reagent box, a second water bath box, a second operation table and a centrifuge; a third sample adding mechanism, a third clamping mechanism, a third reagent box, a sheet baking machine and a third operating platform are arranged in the drip baking cabinet; a fourth clamping mechanism, a fourth reagent box and a fourth water bath box are arranged in the dyeing piece cabinet. The utility model can improve the chromosome preparation efficiency and ensure the accurate diagnosis of the chromosome.

Description

Chromosome preparation device

Technical Field

The utility model belongs to the technical field of medical inspection technology, and particularly relates to a chromosome preparation device.

Background

Chromosome karyotype analysis is particularly important in chromosome disease diagnosis, and along with popularization of chromosome karyotype analysis projects and increase of clinical detection requirements, sample quantity is increased, and requirements on skills of experimenters and chromosome slicing effects are also increased.

Chromosome preparation experiments mainly comprise three links of culture, harvesting and tabletting. At present, the experiments in three links are all manual single operation, are tedious and laborious, have low efficiency, easily cause biological safety accidents in the experimental process, and factors such as manual operation, reagent environment and the like easily cause poor tabletting effect, so that accurate diagnosis of chromosomes is influenced.

Disclosure of Invention

The utility model aims to provide a chromosome preparation device which can save manpower, improve the chromosome preparation efficiency and ensure accurate diagnosis of chromosomes.

The utility model provides a chromosome preparation device which comprises a sample adding cabinet, a culture cabinet, a harvesting cabinet, a dripping baking cabinet, a dyeing piece cabinet and a conveying track, wherein the sample adding cabinet is connected with the culture cabinet; the sample adding cabinet, the culture cabinet, the harvesting cabinet, the drip-baking cabinet and the dyeing cabinet are sequentially arranged, the sample adding cabinet is provided with a sample inlet, the dyeing cabinet is provided with a sample outlet, one end of the conveying track is arranged in the sample adding cabinet and positioned at the sample inlet, and the other end of the conveying track sequentially penetrates through the culture cabinet, the harvesting cabinet, the drip-baking cabinet and the dyeing cabinet and penetrates out of the dyeing cabinet from the sample outlet;

a first sample adding mechanism, a first clamping mechanism, a first reagent box, a first water bath box and a first operation table are arranged in the sample adding cabinet; the harvesting cabinet is internally provided with a second sample adding mechanism, a second clamping mechanism, a second reagent box, a second water bath box, a second operation table and a centrifuge; a third sample adding mechanism, a third clamping mechanism, a third reagent box, a sheet baking machine and a third operating platform are arranged in the drip baking cabinet; and a fourth clamping mechanism, a fourth reagent box and a fourth water bath box are arranged in the dyeing piece cabinet.

In some embodiments, the first sample adding mechanism, the second sample adding mechanism and the third sample adding mechanism comprise a first mechanical arm, a first mounting seat arranged on the first mechanical arm, a first positioning plate arranged on the first mounting seat, a first clamping plate slidingly arranged on the first mounting seat and arranged at intervals with the first positioning plate, a first telescopic device arranged on the first mounting seat and in transmission connection with the first clamping plate, a second telescopic device arranged on the first mounting seat and an extrusion plate in transmission connection with the second telescopic device; a first clamping gap is formed between the first positioning plate and the first clamping plate, and the first clamping gap is located on the moving path of the extrusion plate.

In some embodiments, the first clamping mechanism comprises a second mechanical arm, a second mounting seat arranged on the second mechanical arm, a second positioning plate arranged on the second mounting seat, a second clamping plate arranged on the second mounting seat in a sliding manner and distributed at intervals with the second positioning plate, and a third telescopic device arranged on the second mounting seat and in transmission connection with the second clamping plate; and a second clamping gap is formed between the second positioning plate and the second clamping plate.

In some embodiments, the second clamping mechanism comprises a third mechanical arm, a third mounting seat arranged on the third mechanical arm, a first holding strip hinged on the third mounting seat, a second holding strip hinged on the third mounting seat, a fourth telescopic device arranged on the third mounting seat and a pushing block in transmission connection with the fourth telescopic device. The third arm is not shown. The first holding strip and the second holding strip are respectively provided with a holding end and a driving end, a pipe clamping groove is formed between the holding ends of the first holding strip and the second holding strip, the pushing block is positioned between the driving ends of the first holding strip and the second holding strip, and the driving ends of the first holding strip and the second holding strip are both positioned on the moving path of the pushing block.

In some embodiments, the third clamping mechanism and the fourth clamping mechanism each comprise a fourth mechanical arm, a fourth mounting seat arranged on the fourth mechanical arm, a first motor arranged on the fourth mounting seat, a first screw rod in transmission connection with the first motor, a second screw rod in transmission connection with the first screw rod, a first glass clamping plate in threaded connection with the first screw rod, a second glass clamping plate in threaded connection with the second screw rod, a fifth telescopic device arranged on the fourth mounting seat, and a sucking disc arranged on the fifth telescopic device; the first glass clamping plate and the second glass clamping plate are both arranged on the fourth mounting seat in a sliding mode, threads of the first lead screw and threads of the second lead screw are opposite, the first glass clamping plate and the second glass clamping plate are arranged at intervals, and the fifth telescopic device is arranged between the first glass clamping plate and the second glass clamping plate.

In some embodiments, the first glass clamping plate is provided with a first clamping groove, the second glass clamping plate is provided with a second clamping groove, and the first clamping groove and the second clamping groove are oppositely arranged.

In some embodiments, the centrifuge comprises a drive, a centrifuge disk drivingly connected to the drive; be equipped with a plurality of test tube grooves on the centrifugal disk, a plurality of test tube grooves are followed centrifugal disk's axis of rotation circumference interval distributes.

In some embodiments, a sterilizer is arranged in the sample adding cabinet, a vent hole is formed in the sample adding cabinet, a vent fan is arranged in the vent hole, a waste bucket is arranged in the harvesting cabinet, and material taking openings are formed in the sample adding cabinet and the harvesting cabinet.

In some embodiments, the chromosome preparation apparatus further comprises a first controller provided on the sample addition cabinet, a second controller provided on the harvesting cabinet, a third controller provided on the drip-bake cabinet, and a fourth controller provided on the slice dyeing cabinet; the first controller, the second controller, the third controller, the fourth controller all with delivery track electric connection, first application of sample mechanism first fixture all with first controller electric connection, second application of sample mechanism second fixture centrifuge all with second controller electric connection, third application of sample mechanism third fixture third reagent case roast mascerating machine all with third controller electric connection, fourth fixture with fourth controller electric connection.

In some embodiments, a plurality of standing plates are arranged in the culture cabinet, the plurality of standing plates are sequentially arranged at intervals from top to bottom, and a culture space is formed between every two adjacent standing plates.

The technical scheme provided by the utility model has the following advantages and effects:

according to the utility model, samples are conveyed through the conveying track and sequentially pass through the sample adding cabinet, the culturing cabinet, the harvesting cabinet, the drip baking cabinet and the dyeing cabinet, and experimental links such as culturing, harvesting and flaking are automatically completed by utilizing equipment such as the first sample adding mechanism, the first clamping mechanism, the second sample adding mechanism, the second clamping mechanism, the centrifugal machine, the third sample adding mechanism, the third clamping mechanism, the fourth clamping mechanism and the like, so that manpower can be saved, the efficiency of chromosome preparation can be improved by automatic operation, and accurate diagnosis of chromosomes is ensured.

Drawings

FIG. 1 is a front view of a chromosome preparation apparatus;

FIG. 2 is a schematic structural view of the first loading mechanism or the second loading mechanism or the third loading mechanism;

FIG. 3 is a schematic view of the first clamping mechanism;

FIG. 4 is a schematic view of a second clamping mechanism;

FIG. 5 is a schematic view of the structure of the third clamping mechanism or the fourth clamping mechanism;

reference numerals illustrate:

10. a sample adding cabinet; 11. a culture cabinet; 12. a harvesting cabinet; 13. dripping a baking cabinet; 14. a piece dyeing cabinet; 15. a conveying rail; 16. a sample inlet; 17. a sample outlet; 18. a first sampling mechanism; 19. a first clamping mechanism; 20. a first reagent tank; 21. a first water bath tank; 22. a first operation table; 23. a second sample adding mechanism; 24. a second clamping mechanism; 25. a second reagent tank; 26. a second water bath tank; 27. a second operation table; 28. a centrifuge; 29. standing the plate; 30. a third sample adding mechanism; 31. a third clamping mechanism; 32. a third reagent tank; 33. a sheet baking machine; 34. a third operation table; 35. a fourth clamping mechanism; 36. a fourth reagent tank; 37. a fourth water bath tank; 38. a sterilizer; 39. a vent hole; 40. a ventilation fan; 41. a waste bin; 42. a material taking port; 43. a first controller; 44. a second controller; 45. a third controller; 46. a fourth controller; 47. a timer;

100. a first mechanical arm; 101. a first mount; 102. a first positioning plate; 103. a first clamping plate; 104. a first telescopic device; 105. a second telescopic device; 106. an extrusion plate;

200. a second mechanical arm; 201. a second mounting base; 202. a second positioning plate; 203. a second clamping plate; 204. a third telescopic device;

301. a third mount; 302. a first clip strip; 303. a second clip strip; 304. a fourth retractor; 305. a pushing block; 306. a clamping end; 307. a driving end; 308. a pipe clamping groove;

400. a fourth mechanical arm; 401. a fourth mount; 402. a first motor; 403. a first lead screw; 404. a second lead screw; 405. a first glass clamping plate; 406. a second glass clamping plate; 407. a fifth telescopic device; 408. a suction cup; 409. a first clamping groove; 410. a second clamping groove;

1001. a sixth telescopic device; 1002. swing arms; 1003. a first guide rail; 1004. a first slider; 1005. a rotating seat; 1006. a base; 1007. and a second motor.

Detailed Description

The following describes in further detail the embodiments of the present utility model with reference to the drawings and examples. The following examples are illustrative of the utility model and are not intended to limit the scope of the utility model.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "abutted," "clamped," etc. are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

In the description of the present utility model, unless specifically stated or otherwise defined, it is to be understood that the terms "first," "second," etc. are used in the description of the present utility model to describe various information, but these information should not be limited to these terms, which are used only to distinguish one type of information from another. For example, a "first" message may also be referred to as a "second" message, and similarly, a "second" message may also be referred to as a "first" message, without departing from the scope of the utility model.

As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items, unless specifically stated or otherwise defined.

For convenience of description, unless otherwise specified, the right-left direction hereinafter is identical to the up-down direction of fig. 1 itself, and the up-down direction hereinafter is identical to the right-left direction of fig. 1 itself.

As shown in fig. 1 to 5, a chromosome preparation apparatus includes a sample addition cabinet 10, a culture cabinet 11, a harvesting cabinet 12, a drip-bake cabinet 13, a slice dyeing cabinet 14, and a conveying rail 15. The transport rail 15 is used for transporting the sample. The sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the dyeing cabinet 14 are all provided with cavities, the process flow of chromosome preparation is carried out in the cavities, and the sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the dyeing cabinet 14 are all provided with cabinet doors, so that the safety of chromosome preparation operation is improved. The sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the slice dyeing cabinet 14 are sequentially connected from left to right. The conveying rail 15 may employ a conveying wheel and a conveying belt, and the conveying wheel is driven to rotate by a motor.

The sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the slice dyeing cabinet 14 are sequentially arranged from left to right. The left end of the sample adding cabinet 10 is provided with a sample inlet 16, the right end of the dye piece cabinet 14 is provided with a sample outlet 17, one end of the conveying rail 15 is arranged in the sample adding cabinet 10 and positioned at the sample inlet 16, and a chromosome sample is manually placed on the conveying rail 15 from the sample inlet 16. The other end of the conveying rail 15 sequentially passes through the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the dyeing piece cabinet 14 and passes through the sample outlet 17 to the outside of the dyeing piece cabinet 14, samples can pass through the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the dyeing piece cabinet 14 under the conveying of the conveying rail, and the samples are sent out from the sample outlet 17 after chromosome preparation is completed. The sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the dyeing piece cabinet 14 are sequentially communicated through the rail holes, the flexible strips such as bristles are arranged in the rail holes to separate, and the conveying rail 15 is convenient to pass through while isolating the air among the sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip-baking cabinet 13 and the dyeing piece cabinet 14. The conveying rail 15 sequentially passes through the sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip baking cabinet 13 and the slice dyeing cabinet 14 from the rail holes.

The sample adding cabinet 10 is internally provided with a first sample adding mechanism 18, a first clamping mechanism 19, a first reagent box 20, a first water bath box 21 and a first operation table 22; the first sampling mechanism 18 can grasp an injector, samples in a sample tube are extracted through the injector and added into a culture medium, the first clamping mechanism 19 is used for grasping an inoculation test tube filled with the culture medium, the first reagent box 20 is provided with a semiconductor refrigerating plate for refrigerating, the first reagent box 20 is divided into a plurality of areas, the first reagent box 20 is used for placing frozen reagents such as the culture medium and toxic refrigerated reagents such as colchicine, the culture medium and colchicine are stored in equal areas, and the first clamping mechanism 19 can send the inoculation test tube of the culture medium to the first water bath box 21. The first water bath 21 is used for re-thawing the culture medium or the like, and the first water bath 21 thaws the culture medium or the like with 37 degrees celsius constant temperature water. The first station 22 is for placement of an inoculating tube containing a culture medium. In use, the sample tube containing the sample is transported through the conveyor track 15, the first gripper mechanism 19 grips the sample onto the first station 22 and grips the inoculated test tube containing the reconstituted culture medium onto the first station 22. The first sampling mechanism 18 sucks the sample of the sample tube by a syringe and adds the sample to the inoculation test tube filled with the culture medium, completes sample inoculation, and then places the inoculated sample on the conveying rail 15 through the first clamping mechanism 19 and conveys the sample to the culture cabinet 11.

The harvesting cabinet 12 is internally provided with a second sample adding mechanism 23, a second clamping mechanism 24, a second reagent box 25, a second water bath box 26, a second operating platform 27 and a centrifuge 28. The second reagent tank 25 contains reagents including a fixing solution, a hypotonic solution, and the like. The cultured sample (contained in a test tube) is transported to the harvest tank 12 via the transport rail 15. The second gripper mechanism 24 grips the test tube with the sample and places it on the second station 27. The second sampling mechanism 23 has the same function as the first sampling mechanism 18, and the second sampling mechanism 23 is used for grabbing a syringe to inject reagent into an inoculated test tube, extracting waste liquid, and blowing samples in the test tube. After the reagent is added into the sample in the test tube, the sample is clamped by the second clamping mechanism 24 and placed in the second water bath 26 for hypotonic, and then the second clamping mechanism 24 clamps the test tube to centrifuge the hypotonic sample in the centrifuge 28, so that the sample harvesting process is completed. After centrifugation is completed, the second clamping mechanism 24 clamps the test tube to place the sample in the conveying track 15 and convey the sample into the drip-bake cabinet 13.

The drip baking cabinet 13 is internally provided with a third sampling mechanism 30, a third clamping mechanism 31, a third reagent box 32, a sheet baking machine 33 and a third operating platform 34. The sheet baking machine 33 is a conventional device, and the third reagent box 32 is used for storing reagents such as cell suspensions. After the tube containing the centrifuged sample is transferred to the drip-bake oven 13, the third clamping mechanism 31 places the glass sheet on the third operating table 34, the third sampling mechanism 30 draws the sample, the cell suspension through the syringe, mixes it, and drips onto the glass sheet, and immediately after the third clamping mechanism 31 places the glass sheet on the sheet-bake machine 33, and the sheet is baked at ninety degrees celsius for one hour.

The fourth clamping mechanism 35, the fourth reagent box 36 and the fourth water bath box 37 are arranged in the dyeing piece cabinet 14. The fourth reagent tank 36 stores the reagent for dyeing, and the fourth water bath tank 37 has three areas for heating the reagent in the water bath. After the glass sheet is conveyed into the sheet dyeing cabinet 14 by the conveying track 15, the fourth clamping mechanism 35 clamps the glass sheet to a designated place, and then the reagent is injected into the glass sheet by the injector for dyeing, and the injection process can be performed manually or by adopting any one of the first sample adding mechanism, the second sample adding mechanism and the third sample adding mechanism. Finally, the dyed glass sheet is held by the fourth holding mechanism 35, put on the conveying rail 15, and finally sent out from the sample outlet 17.

In one embodiment, the first sampling mechanism 18, the second sampling mechanism 23, and the third sampling mechanism 30 each include a first mechanical arm 100, a first mounting seat 101 disposed on the first mechanical arm 100, a first positioning plate 102 disposed on the first mounting seat 101, a first clamping plate 103 slidably disposed on the first mounting seat 101 and spaced from the first positioning plate 102, a first telescopic device 104 disposed on the first mounting seat 101 and in transmission connection with the first clamping plate 103, a second telescopic device 105 disposed on the first mounting seat 101, and a squeezing plate 106 in transmission connection with the second telescopic device 105; the first positioning plate 102 and the first clamping plate 103 have a first clamping gap therebetween, and the first clamping gap is located on the moving path of the pressing plate 106. The first telescopic device 104 is used for pushing the first clamping plate 103 to move close to or far away from the first positioning plate 102, so that the size of the first clamping gap is changed, the injector is clamped between the first clamping plate 103 and the first positioning plate 102, after the injector is clamped by the first clamping plate 103 and the first positioning plate 102, the piston of the injector is clamped with the extrusion plate 106, and the extrusion plate 106 can drive the piston of the injector to slide under the pushing of the second telescopic device 105, so that the injector can extract or inject reagents.

The first telescopic device 104, the first clamping plate 103 and the first positioning plate 102 are provided with a plurality of groups, so that a plurality of syringes can be used for simultaneously carrying out sample adding operation, and the efficiency is improved.

In one embodiment, the first clamping mechanism 19 includes a second mechanical arm 200, a second mounting seat 201 disposed on the second mechanical arm 200, a second positioning plate 202 disposed on the second mounting seat 201, a second clamping plate 203 slidingly disposed on the second mounting seat 201 and spaced from the second positioning plate 202, and a third telescopic device 204 disposed on the second mounting seat 201 and in transmission connection with the second clamping plate 203; a second clamping gap is formed between the second positioning plate 202 and the second clamping plate 203. The first clamping mechanism 19 lacks only the squeeze plate compared to the first 18 or second 23 or third 30 sampling mechanisms, and the rest of the structure and principle are the same.

In one embodiment, the second clamping mechanism 24 includes a third mechanical arm, a third mounting base 301 disposed on the third mechanical arm, a first clamping strip 302 hinged on the third mounting base 301, a second clamping strip 303 hinged on the third mounting base 301, a fourth telescopic device 304 mounted on the third mounting base 301, and a pushing block 305 in driving connection with the fourth telescopic device 304. The first clamping strip 302 and the second clamping strip 303 are pivotally connected to the third mounting base 301 through a pivot. The first clip strip 302 and the second clip strip 303 each have a clip end 306 and a drive end 307, with a pivot axis located between the clip end 306 and the drive end 307. A pipe clamping groove 308 is formed between the clamping end 306 of the first clamping strip 302 and the clamping end 306 of the second clamping strip 303, semicircular grooves are formed in the clamping end 306 of the first clamping strip 302 and the clamping end 306 of the second clamping strip 303, and the two semicircular grooves form the pipe clamping groove 308. The pushing block 305 is located between the driving end 307 of the first holding strip 302 and the driving end 307 of the second holding strip 303, and both the driving end 307 of the first holding strip 302 and the driving end 307 of the second holding strip 303 are located on the moving path of the pushing block 305, when the pushing block 305 pushes the driving end 307 of the first holding strip 302 and the driving end 307 of the second holding strip 303, the clamping end 306 of the first holding strip 302 and the clamping end 306 of the second holding strip 303 are close to each other and clamp the test tube.

In one embodiment, the third clamping mechanism 31 and the fourth clamping mechanism 35 each comprise a fourth mechanical arm 400, a fourth mounting seat 401 arranged on the fourth mechanical arm 400, a first motor 402 mounted on the fourth mounting seat 401, a first lead screw 403 in transmission connection with the first motor 402, a second lead screw 404 in transmission connection with the first lead screw 403, a first glass clamping plate 405 in threaded connection with the first lead screw 403, a second glass clamping plate 406 in threaded connection with the second lead screw 404, a fifth telescopic device 407 arranged on the fourth mounting seat 401, and a sucking disc 408 arranged on the fifth telescopic device 407. The movement directions of the first glass clamping plate 405 and the second glass clamping plate 406 are perpendicular to the expansion and contraction direction of the fifth expansion and contraction device 407. The first glass clamping plate 405 and the second glass clamping plate 406 are both slidably arranged on the fourth mounting seat 401, and the threads of the first screw 403 and the second screw 404 are opposite, so that the movement directions of the first glass clamping plate 405 and the second glass clamping plate 406 are opposite when the first screw 403 and the second screw 404 rotate in the same direction, and clamping and releasing of glass sheets are realized. The first glass clamping plate 405 and the second glass clamping plate 406 are arranged at intervals, and the fifth expansion device 407 is arranged between the first glass clamping plate 405 and the second glass clamping plate 406. The fifth retractors 407 and the suckers 408 are in one-to-one correspondence, the fourth mechanical arm 400 drives the fourth mounting seat 401 to move to the glass sheet to be discharged, the fifth retractors 407 extend out, the suckers 408 suck the periphery of the glass sheet to enable the glass sheet to rise to a certain height, then the first glass clamping plate 405 and the second glass clamping plate 406 are close to each other to clamp the glass sheet, and then the suckers 408 continue to rise to leave the glass sheet.

In one embodiment, the first robot arm 100, the second robot arm 200, the third robot arm, and the fourth robot arm 400 may use existing six-degree-of-freedom robot arms.

In one embodiment, the first mechanical arm 100, the second mechanical arm 200, the third mechanical arm, and the fourth mechanical arm 400 each include a sixth telescopic device 1001, a swing arm 1002, a first guide rail 1003, a first slider 1004, a rotating base 1005, a base 1006, and a second motor 1007. The first guide rails 1003 are arranged at the tops of the sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip baking cabinet 13 and the slice dyeing cabinet 14, and when the sample adding cabinet 10, the culture cabinet 11, the harvesting cabinet 12, the drip baking cabinet 13 and the slice dyeing cabinet 14 are provided with a plurality of first guide rails 1003, the first guide rails 1003 are arranged at intervals front and back, so that horizontal left-right movements of all mechanisms are not interfered with each other. The first guide rail 1003 has a screw driven by a third motor, and the first slider 1004 is screwed to the screw to realize sliding. The rotating base 1005 is mounted on the first slider 1004 and is driven to rotate by a fourth motor (not shown), and the rotation axis of the rotating base 1005 is disposed vertically. The sixth expansion piece 1001, swing arm 1002 all have a plurality ofly, and a plurality of swing arms 1002 are articulated in proper order, and the swing arm 1002 of one end articulates on rotating seat 1005, and the swing arm 1002 of the other end is equipped with base 1006, is equipped with second motor 1007 on the base 1006, and second motor 1007 is connected with first mount pad 101 or second mount pad 201 or third mount pad 301 or fourth mount pad 401 transmission.

In one embodiment, the first, second, third, fourth, fifth and sixth retractors 104, 105, 204, 304, 407, 1001 are air or hydraulic cylinders or electric telescopic rods.

In one embodiment, the first glass clamping plate 405 is provided with a first clamping groove 409, the second glass clamping plate 406 is provided with a second clamping groove 410, and the first clamping groove 409 and the second clamping groove 410 are disposed opposite to each other. The first clamping groove 409 and the second clamping groove 410 enable the glass sheet to be clamped stably, and prevent the glass sheet from sliding down.

In one embodiment, the centrifuge 28 includes a drive, a centrifuge disk drivingly connected to the drive. The driver is a fifth motor, a plurality of test tube grooves are formed in the centrifugal disc, and the test tube grooves are circumferentially distributed at intervals along the rotation axis of the centrifugal disc.

In one embodiment, the sample adding cabinet 10 is provided with a sterilizer 38, the sterilizer 38 automatically sprays sterilizing gas or solution at regular time, and the sterilizer 38 is of an existing structure. The sample adding cabinet 10 is provided with a vent hole 39, a vent fan 40 is arranged in the vent hole 39, the environmental requirement of the sample adding cabinet 10 during inoculation is required to be consistent with that of the biosafety cabinet, and the sample adding cabinet has a ventilation function and a sterilization function, so that the experiment failure caused by pollution in the inoculation process is prevented. The harvesting cabinet 12 is internally provided with a waste bucket 41, and the second sample adding mechanism 23 extracts waste liquid by using a syringe and puts the waste liquid into the waste bucket 41 for recycling. The sample adding cabinet 10 and the harvesting cabinet 12 are respectively provided with a material taking opening 42, after the samples of the sample tubes in the sample adding cabinet 10 are added to the culture medium, the sample tubes are taken out from the material taking openings 42, and the residual cell suspension in the harvesting cabinet 12 is taken out from the material taking openings 42.

In one embodiment, the chromosome preparation apparatus further comprises a first controller 43 disposed on the loading bin 10, a second controller 44 disposed on the harvesting bin 12, a third controller 45 disposed on the drip-bake bin 13, and a fourth controller 46 disposed on the slice dyeing bin 14. The first controller 43, the second controller 44, the third controller 45, and the fourth controller 46 are all electrically connected to the conveying track 15, and can respectively control the start and stop of the conveying track 15. The first sampling mechanism 18 and the first clamping mechanism 19 are electrically connected with the first controller 43, and the first controller 43 can control the first sampling mechanism 18, the first clamping mechanism 19, the first water bath 21 and other devices to operate. The second loading mechanism 23, the second clamping mechanism 24, and the centrifuge 28 are electrically connected to the second controller 44, and the second controller 44 may control the second loading mechanism 23, the second clamping mechanism 24, the centrifuge 28, and other devices to operate. The third sampling mechanism 30, the third clamping mechanism 31, the third reagent box 32, the sheet baking machine 33 are electrically connected with the third controller 45, and the third controller 45 controls the third sampling mechanism 30, the third clamping mechanism 31, the third reagent box 32, the sheet baking machine 33 and other devices to operate. The fourth clamping mechanism 35 is electrically connected to the fourth controller 46, and the fourth controller 46 can control the fourth clamping mechanism 35 and other devices to operate. The incubator 11 is provided with a timer 47 for counting the incubation time.

In one embodiment, a plurality of standing plates 29 are disposed in the culture cabinet 11, and the plurality of standing plates 29 are sequentially arranged at intervals from top to bottom, and a culture space is formed between adjacent standing plates 29. The inoculated samples are conveyed to the culture cabinet 11 through the conveying track 15, are grabbed and placed on the standing plate 29 in the culture cabinet 11 through a manual or robot to be cultured for sixty-eight hours to seventy-two hours, the standing plate 29 is divided into a plurality of layers, the inoculated samples are placed in layers, and each layer is manually or slightly rocked for five minutes every twelve hours, so that the samples are fully cultured.

The working principle of the application is as follows:

the first step: the chromosome preparation device is started, and various reagent areas are melted in a water bath. Samples are transported from the sample inlet 16 through the transport rail 15 into the loading bay 10. The first clamping mechanism 19 places the sample and the culture medium on the first operation table 22, and the first sampling mechanism 18 sucks the sample into the culture medium and mixes them uniformly to complete the inoculation work.

In the process, the culture medium is redissolved in advance, a sterilized sample enters the sample adding cabinet through the sample inlet, the first sample adding mechanism is provided with the injector in advance, the injector is inoculated into the culture medium from the sucked sample (0.5 ml of peripheral blood), the first clamping mechanism is uniformly shaken and then put into the conveying track, the conveying track is moved into the culture cabinet for culture, the culture time is set, and the automatic and light mixing is performed every twenty-four hours.

And a second step of: the inoculated culture medium is transported to the culture cabinet 11 for culture through the conveying rail 15, and is transported to the sample adding cabinet 10 again through the conveying rail 15 1 hour before the culture is finished, the first clamping mechanism 19 grabs the sample to the first operation table 22, and the first sampling mechanism 18 continues to send back to the culture cabinet 11 for culture after colchicine is added.

And a third step of: after the cultivation is finished, the culture medium is transported into a harvesting box through a conveying rail 15 to perform a harvesting procedure, wherein the harvesting procedure comprises hypotonic, fixing, centrifugation and the like.

In the process, the second sampling mechanism is utilized to add the needed reagents such as hypotonic solution, fixed solution and the like in advance, the liquid in the culture bottle is automatically moved into the test tube through the second sampling mechanism and the second clamping mechanism, 8ml of KCl solution preheated in water bath is automatically added, and the materials are fully and uniformly mixed. Hypotonic for 30 minutes in a 37℃water bath. And then the second sampling mechanism automatically adds 2ml of fixing solution and uniformly mixes the fixing solution, the second clamping mechanism moves the mixed solution to a centrifugal area for centrifugation, and the second sampling mechanism removes the supernatant and then adds 8ml of fixing solution and lightly mixes the fixing solution to prepare the cell suspension. Finally, the cell suspension is automatically moved into the drip-baking cabinet through the cooperation of the second clamping mechanism and the conveying track.

Fourth step: after harvesting, the cells are moved into a drip-baking cabinet 13 through a conveying rail 15, and cell suspensions are configured to perform procedures such as drip-sheet and baking-sheet.

Fifth step: then, after the baking sheet is finished, the baking sheet is conveyed into a sheet dyeing cabinet 14 through a conveying rail 15 for sheet dyeing, and finally, the prepared dyed glass sheet is sent out from a sample outlet 17 through the conveying rail 15, so that chromosome preparation is completed.

Overall, the advantages of the present application are: the whole experiment reduces manual operation, reduces the labor intensity of experimenters and improves the efficiency. The experimental process is uniform in operation, the environmental temperature and humidity and the like are easy to control, and poor chromosome tabletting effect or instability cannot be caused.

The above examples are also not an exhaustive list based on the utility model, and there may be a number of other embodiments not listed. Any substitutions and modifications made without departing from the spirit of the utility model are within the scope of the utility model.

Claims (10)

1. The chromosome preparation device is characterized by comprising a sample adding cabinet, a culture cabinet, a harvesting cabinet, a dripping baking cabinet, a dyeing piece cabinet and a conveying track; the sample adding cabinet, the culture cabinet, the harvesting cabinet, the drip-baking cabinet and the dyeing cabinet are sequentially arranged, the sample adding cabinet is provided with a sample inlet, the dyeing cabinet is provided with a sample outlet, one end of the conveying track is arranged in the sample adding cabinet and positioned at the sample inlet, and the other end of the conveying track sequentially penetrates through the culture cabinet, the harvesting cabinet, the drip-baking cabinet and the dyeing cabinet and penetrates out of the dyeing cabinet from the sample outlet;

a first sample adding mechanism, a first clamping mechanism, a first reagent box, a first water bath box and a first operation table are arranged in the sample adding cabinet; the harvesting cabinet is internally provided with a second sample adding mechanism, a second clamping mechanism, a second reagent box, a second water bath box, a second operation table and a centrifuge; a third sample adding mechanism, a third clamping mechanism, a third reagent box, a sheet baking machine and a third operating platform are arranged in the drip baking cabinet; and a fourth clamping mechanism, a fourth reagent box and a fourth water bath box are arranged in the dyeing piece cabinet.

2. The chromosome preparation apparatus according to claim 1, wherein the first loading mechanism, the second loading mechanism, and the third loading mechanism each comprise a first mechanical arm, a first mounting seat provided on the first mechanical arm, a first positioning plate provided on the first mounting seat, a first clamping plate slidingly provided on the first mounting seat and arranged at intervals from the first positioning plate, a first telescopic device provided on the first mounting seat and in transmission connection with the first clamping plate, a second telescopic device provided on the first mounting seat, and a squeeze plate in transmission connection with the second telescopic device; a first clamping gap is formed between the first positioning plate and the first clamping plate, and the first clamping gap is located on the moving path of the extrusion plate.

3. The chromosome preparation apparatus according to claim 1, wherein the first clamping mechanism comprises a second mechanical arm, a second mounting seat arranged on the second mechanical arm, a second positioning plate arranged on the second mounting seat, a second clamping plate slidingly arranged on the second mounting seat and arranged at intervals with the second positioning plate, and a third telescopic device arranged on the second mounting seat and in transmission connection with the second clamping plate; and a second clamping gap is formed between the second positioning plate and the second clamping plate.

4. The chromosome preparation apparatus according to claim 1, wherein the second clamping mechanism comprises a third mechanical arm, a third mounting base arranged on the third mechanical arm, a first holding strip hinged on the third mounting base, a second holding strip hinged on the third mounting base, a fourth telescopic device arranged on the third mounting base, and a pushing block in transmission connection with the fourth telescopic device; the first holding strip and the second holding strip are respectively provided with a holding end and a driving end, a pipe clamping groove is formed between the holding ends of the first holding strip and the second holding strip, the pushing block is positioned between the driving ends of the first holding strip and the second holding strip, and the driving ends of the first holding strip and the second holding strip are both positioned on the moving path of the pushing block.

5. The chromosome preparation apparatus according to claim 1, wherein the third clamping mechanism and the fourth clamping mechanism each comprise a fourth mechanical arm, a fourth mounting seat arranged on the fourth mechanical arm, a first motor arranged on the fourth mounting seat, a first lead screw in driving connection with the first motor, a second lead screw in driving connection with the first lead screw, a first glass clamping plate in driving connection with the first lead screw, a second glass clamping plate in driving connection with the second lead screw, a fifth telescopic arranged on the fourth mounting seat, and a sucking disc arranged on the fifth telescopic; the first glass clamping plate and the second glass clamping plate are both arranged on the fourth mounting seat in a sliding mode, threads of the first lead screw and threads of the second lead screw are opposite, the first glass clamping plate and the second glass clamping plate are arranged at intervals, and the fifth telescopic device is arranged between the first glass clamping plate and the second glass clamping plate.

6. The apparatus according to claim 5, wherein the first glass-clamping plate is provided with a first clamping groove, the second glass-clamping plate is provided with a second clamping groove, and the first clamping groove and the second clamping groove are arranged opposite to each other.

7. The chromosome preparation apparatus of claim 1, wherein the centrifuge comprises a drive, a centrifuge disk drivingly connected to the drive; be equipped with a plurality of test tube grooves on the centrifugal disk, a plurality of test tube grooves are followed centrifugal disk's axis of rotation circumference interval distributes.

8. The chromosome preparation apparatus according to any one of claims 1 to 7, wherein a sterilizer is provided in the sample addition cabinet, a vent hole is provided in the sample addition cabinet, a vent fan is provided in the vent hole, a waste bucket is provided in the harvesting cabinet, and material taking openings are provided in both the sample addition cabinet and the harvesting cabinet.

9. The chromosome preparation apparatus of any one of claims 1 to 7, further comprising a first controller disposed on the loading bin, a second controller disposed on the harvesting bin, a third controller disposed on the drip-bake bin, a fourth controller disposed on the slice dyeing bin; the first controller, the second controller, the third controller, the fourth controller all with delivery track electric connection, first application of sample mechanism first fixture all with first controller electric connection, second application of sample mechanism second fixture centrifuge all with second controller electric connection, third application of sample mechanism third fixture third reagent case roast mascerating machine all with third controller electric connection, fourth fixture with fourth controller electric connection.

10. The chromosome preparation apparatus according to any one of claims 1 to 7, wherein a plurality of stationary plates are provided in the culture cabinet, the plurality of stationary plates are sequentially arranged at intervals from top to bottom, and a culture space is formed between adjacent stationary plates.