CN213447099U

CN213447099U - Cell bioreactor

Info

Publication number: CN213447099U
Application number: CN202022107405.9U
Authority: CN
Inventors: 张奎
Original assignee: Guangxi Zhuoqiang Technology Co ltd
Current assignee: Guangxi Zhuoqiang Technology Co ltd
Priority date: 2020-09-23
Filing date: 2020-09-23
Publication date: 2021-06-15
Anticipated expiration: 2030-09-23

Abstract

The utility model discloses a cell bioreactor relates to reactor technical field, including motor and lid, the output of motor is rotated and is connected with stirring vane, stirring vane's fixed surface all around is connected with the puddler, lid clearance fit is connected with the PH electrode, the output wire of PH electrode is connected with PH electrode interface, lid clearance fit is connected with the pipe, lid clearance fit is connected with temperature sensor. The utility model discloses in, use through the cooperation between pipe, peristaltic pump, shell, motor, puddler, stirring vane and the lid, the homogeneous mixing cell biology and the culture solution that has configured use through the cooperation between switch, temperature sensor, display screen, the heating pad, let the temperature in the shell suitable, use through the cooperation between lid and the microscope, observe the change of its biological cell, increased the convenience of observing its change.

Description

Cell bioreactor

Technical Field

The utility model relates to a reactor technical field specifically is a cell bioreactor.

Background

When animal cells are cultured in vitro, the bioreactor is a key device in the whole culture process, and provides a proper growth environment for the cells, so that the cells can rapidly proliferate and form a required biological tissue product. Since animal cells are different from microbial cells in terms of morphological structure, culture method, required mechanical environment and the like, the traditional microbial reactor is obviously not suitable for large-scale culture of animal cells, particularly the requirement of tissue engineering, and the research and development of a novel bioreactor are promoted. All cell cultures require the use of a cell bioreactor.

In the prior art, most cell bioreactors are not integrated with a microscope, which reduces the convenience of observing cell biology.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell bioreactor to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

a cell bioreactor comprises a motor and a cover, wherein the output end of the motor is rotatably connected with stirring blades, the peripheral surfaces of the stirring blades are fixedly connected with stirring rods, the cover is in clearance fit with a PH electrode, the output end lead of the PH electrode is connected with a PH electrode interface, the cover is in clearance fit with a pipe, the cover is in clearance fit with a temperature sensor, the output end lead of the temperature sensor is connected with a temperature sensor interface, the bottom of the cover is provided with a shell, the bottom of the cover is provided with the shell, the periphery of the shell is fixedly connected with a heating pad, the output end lead of the heating pad is connected with a power supply connector, the bottom of the shell is fixedly connected with a refrigerating sheet, the bottom of the refrigerating sheet is fixedly connected with a placing plate, the top of the placing plate is fixedly connected with a, and one side of the placing plate is fixedly connected with a controller, the front side of the controller is fixedly connected with a switch, the front side of the controller is fixedly connected with a display screen, and the top of the controller is fixedly connected with a microscope.

As a further aspect of the present invention: the bottom fixedly connected with lid of motor, the puddler runs through in the lid, the puddler passes through clearance fit with the lid and is connected, stirring vane evenly sets up the surface all around at the puddler.

As a further aspect of the present invention: the peristaltic pump is characterized in that a pipe is arranged inside the peristaltic pump, the pipe penetrates through the cover, a sealing ring is arranged at the contact position of the pipe and the cover, and the pipe is communicated with the inside of the shell.

As a further aspect of the present invention: the PH electrode runs through in the lid, PH electrode and lid contact department are provided with the sealing washer, temperature sensor runs through in the lid, temperature sensor and lid contact department are provided with the sealing washer.

As a further aspect of the present invention: and the power supply connector, the temperature sensor interface and the PH electrode interface are positioned on one side of the controller close to the motor.

As a further aspect of the present invention: the inside deposit power that is provided with of motor and peristaltic pump, the switch is external to have the power, PH electrode, temperature sensor and heating pad and switch all pass through wire electric connection.

Compared with the prior art, the beneficial effects of the utility model are that: through the cooperation use between pipe, peristaltic pump, shell, motor, puddler, stirring vane and the lid, the homogeneous mixing cell biology and the culture solution that has configured, use through the cooperation between switch, temperature sensor, display screen, the heating pad, let the temperature in the shell suitable, use through the cooperation between lid and the microscope, observe its biological cell's change, increased the convenience of observing its change.

Drawings

FIG. 1 is a schematic diagram of a cell bioreactor.

FIG. 2 is a sectional view of a cell bioreactor.

FIG. 3 is a plan view of a stirring rod and a stirring blade in a cell bioreactor.

The labels in the figure are: 1. a controller; 2. a switch; 3. a display screen; 4. a microscope; 5. a temperature sensor; 6. a motor; 7. a PH electrode; 8. a tube; 9. a housing; 10. a power supply connector; 11. a heating pad; 12. a peristaltic pump; 13. placing the plate; 14. a refrigeration plate; 15. a stirring rod; 16. a stirring blade; 17. a temperature sensor interface; 18. a PH electrode interface; 19. and a cover.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 3, in an embodiment of the present invention, a cell bioreactor includes a motor 6 and a cover 19, an output end of the motor 6 is rotatably connected with a stirring blade 16, a stirring rod 15 is fixedly connected to the peripheral surface of the stirring blade 16, the cover 19 is connected with a PH electrode 7 in a clearance fit manner, an output end lead of the PH electrode 7 is connected with a PH electrode interface 18, the cover 19 is connected with a pipe 8 in a clearance fit manner, the cover 19 is connected with a temperature sensor 5 in a clearance fit manner, an output end lead of the temperature sensor 5 is connected with a temperature sensor interface 17, a housing 9 is arranged at the bottom of the cover 19, the housing 9 is arranged at the bottom of the cover 19, a heating pad 11 is fixedly connected to the periphery of the housing 9, an output end lead of the heating pad 11 is connected with a power supply connector 10, a refrigerating sheet 14 is, place top fixedly connected with peristaltic pump 12 of board 13, and place one side fixedly connected with controller 1 of board 13, the front side fixedly connected with switch 2 of controller 1, the front side fixedly connected with display screen 3 of controller 1, and the top fixedly connected with microscope 4 of controller 1.

Referring to fig. 1, 2 and 3, a cover 19 is fixedly connected to the bottom of the motor 6, the stirring rod 15 penetrates through the cover 19, the stirring rod 15 and the cover 19 are connected in a clearance fit manner, and the stirring blades 16 are uniformly arranged on the peripheral surface of the stirring rod 15 to uniformly mix the cell organisms and the prepared culture solution.

Referring to fig. 1 and 2, a tube 8 is disposed inside the peristaltic pump 12, the tube 8 penetrates through the cover 19, a sealing ring is disposed at a contact position of the tube 8 and the cover 19, the tube 8 is communicated with the inside of the housing 9, and the prepared culture solution is injected into the housing 9 and is fully mixed with the cell organisms.

Referring to fig. 1 and 2, the PH electrode 7 penetrates the cover 19, a sealing ring is disposed at a contact position of the PH electrode 7 and the cover 19, the temperature sensor 5 penetrates the cover 19, and the sealing ring is disposed at a contact position of the temperature sensor 5 and the cover 19 to detect a PH value change of the biological cell.

Referring to fig. 1 and 2, the power connector 10, the temperature sensor interface 17 and the PH electrode interface 18 are disposed on a side of the controller 1 adjacent to the motor 6.

Referring to fig. 1 and 2, a storage power supply is arranged inside the motor 6 and the peristaltic pump 12, the switch 2 is externally connected with the power supply, and the PH electrode 7, the temperature sensor 5, the heating pad 11 and the switch 2 are electrically connected through wires.

The utility model discloses a theory of operation is: in the using process, the tube 8 is placed in a test tube cup, the peristaltic pump 12 is debugged and started, the prepared culture solution is injected into the shell 9, the motor 6 is started, the motor 6 drives the stirring rod 15 to rotate, the stirring blades 16 rotate along with the stirring rod to uniformly mix the cell organisms and the prepared culture solution, in the using process, the temperature of the temperature sensor 5 of the switch 2 is turned on and displayed through the display screen 3, the temperature of the heating pad 11 is adjusted according to the temperature change, the temperature in the shell 9 is proper, and in the using process, the cover 19 is periodically opened, and some biological cells are taken out and placed on a microscope to observe the change of the biological cells.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A cell bioreactor comprising a motor (6) and a cover (19), characterized in that: the output of motor (6) rotates and is connected with stirring vane (16), stirring vane (16) fixed surface all around is connected with puddler (15), lid (19) clearance fit is connected with PH electrode (7), the output wire of PH electrode (7) is connected with PH electrode interface (18), lid (19) clearance fit is connected with pipe (8), lid (19) clearance fit is connected with temperature sensor (5), the output wire of temperature sensor (5) is connected with temperature sensor interface (17), the bottom of lid (19) is provided with shell (9), and the bottom of lid (19) is provided with shell (9), the fixed connection all around of shell (9) has heating pad (11), the output wire of heating pad (11) is connected with power connector (10), the bottom fixed connection of shell (9) has refrigeration piece (14), the bottom fixedly connected with of refrigeration piece (14) places board (13), place top fixedly connected with peristaltic pump (12) of board (13), just one side fixedly connected with controller (1) of placing board (13), the front side fixedly connected with switch (2) of controller (1), the front side fixedly connected with display screen (3) of controller (1), just the top fixedly connected with microscope (4) of controller (1).

2. A cell bioreactor according to claim 1, wherein: the bottom fixedly connected with lid (19) of motor (6), puddler (15) run through in lid (19), puddler (15) are connected through clearance fit with lid (19), stirring vane (16) evenly set up the surface all around of puddler (15).

3. A cell bioreactor according to claim 1, wherein: a pipe (8) is arranged in the peristaltic pump (12), the pipe (8) penetrates through the cover (19), a sealing ring is arranged at the contact position of the pipe (8) and the cover (19), and the pipe (8) is communicated with the inside of the shell (9).

4. A cell bioreactor according to claim 1, wherein: PH electrode (7) run through in lid (19), PH electrode (7) and lid (19) contact department are provided with the sealing washer, temperature sensor (5) run through in lid (19), temperature sensor (5) and lid (19) contact department are provided with the sealing washer.

5. A cell bioreactor according to claim 1, wherein: the power connector (10), the temperature sensor interface (17) and the PH electrode interface (18) are positioned on one side of the controller (1) close to the motor (6).

6. A cell bioreactor according to claim 1, wherein: the storage power supply is arranged inside the motor (6) and the peristaltic pump (12), the switch (2) is externally connected with the power supply, and the PH electrode (7), the temperature sensor (5), the heating pad (11) and the switch (2) are all electrically connected through wires.