CN107864932A - Use for laboratory drosophila cultivates and doser - Google Patents
Use for laboratory drosophila cultivates and doser Download PDFInfo
- Publication number
- CN107864932A CN107864932A CN201711270574.0A CN201711270574A CN107864932A CN 107864932 A CN107864932 A CN 107864932A CN 201711270574 A CN201711270574 A CN 201711270574A CN 107864932 A CN107864932 A CN 107864932A
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- China
- Prior art keywords
- epimere
- mouth
- hypomere
- drosophila
- stage casing
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The present invention relates to the culture of use for laboratory drosophila and doser, belongs to use for laboratory drosophila experimental provision technical field, drosophila culture needs to change the technical problems such as different blake bottles from administration process in the prior art for solution, and solution is：Mouth is connected through a screw thread respectively between epimere and stage casing, between stage casing and hypomere；Epimere upper end surface is provided with bottle stopper, and decoction adding tube connects through bottle stopper with epimere, and the middle part of epimere side wall is provided with anesthetic air inlet one；The bottom of hypomere is provided with culture medium, and the middle part of hypomere side wall is provided with anesthesia gas air inlet two；Sieve plate is provided with threaded connection mouth one, is threadedly coupled at mouth two and is provided with baffle plate, handle drives 9 o-clock positions to 12 o-clock positions of baffle plate or sieve plate in pointer circular rotating track to rotate by rotating shaft.Present arrangement avoids do drosophila administration experimentation in need multiple links and different blake bottles the problem of, there is provided the integrated experimental provision of too many levels.
Description
Technical field
The invention belongs to use for laboratory drosophila experimental provision technical field, the more particularly to culture of use for laboratory drosophila and administration
Device.
Background technology
Drosophila be classical mode biology, have the cycle it is short, it is easy raising, cost is low, breeding is fast, individual is small, Hou Daiduo, dyeing
The advantages such as body is few, character is obvious, genetic homology, be widely used in science of heredity and auxology correlative study, Parkinson's and Ah
The research and development of the nervous system disease agents such as Alzheimer's disease, drug habit, aging and life-span, sleep and biological rhythm, learning and memory
Etc. research field.
Research treatment disease medicament screening experiment in, through frequently with drosophila model organism as experimental subjects.Typically
Needed according to experiment and medicine feature is using pharmaceutical culture medium or the method for pipette tips administration.Adopt sometimes in experimentation by
The method that medicine is mixed in culture medium carries out pharmaceutical intervention and treatment, and experimental implementation is more complicated, and being required for every time will be suitable
The medicine of ratio is dissolved into culture medium.Using pipette tips medication, the step of making pharmaceutical culture medium, but this method are avoided
The step of being related to is relatively more, it is necessary to by multiple links such as feeding-empty stomach-administration-feedings, and each link is required for fruit
Fly is poured into different blake bottles, such as：Drosophila is poured into the blake bottle of sky from the blake bottle equipped with culture medium, it is small to stop 2
When allow drosophila to be in starvation；Drosophila is poured into another blake bottle again, drug injection is entered in pipette tips, pipette tips plug is existed
In blake bottle tampon, drosophila is fed；Also need to pour into drosophila in the blake bottle equipped with culture medium again afterwards.
In a word, at present the conventional pipette tips medication in laboratory the shortcomings that be：
1st, it is frangible using glass blake bottle, often occur injuring caused by experimenter because blake bottle is broken in experimentation,
The cost of experiment is also increased simultaneously.
2nd, it is necessary to which multiple links, each link such as feeding-empty stomach-administration-feeding are required for drosophila in administration process
Different blake bottles is poured into, it is necessary to spend the substantial amounts of time and efforts of experimenter.
3rd, the different blake bottles needed with a collection of drosophila need to carry out identical mark, cause very big workload, and
Easily there are the errors such as the blake bottle for drosophila being poured into mistake, influence the accuracy of experimental result.
4th, check and verify and tested, it is necessary to which the quantity of blake bottle is many for large batch of drosophila drug sieve, the very big experiment of occupancy is empty
Between, influence the progress of other experiments.
5th, drug sieve, which is checked and verify to test, needs substantial amounts of drosophila blake bottle, during cleaning blake bottle etc., largely occupies treasured
Expensive experimental period and manpower.
6th, during administration, it is necessary to drosophila is poured into different blake bottles, often result in drosophila and fly out experimental provision, shadow
Ring the quantity of experiment drosophila and the accuracy of experiment.
7th, existing administration pipette tips do not have reference mark, are unfavorable for recording the amount of drosophila intake medicine, influence experimental result
Analysis.
The content of the invention
The defects of it is an object of the invention to overcome prior art, solve drosophila culture in the prior art needs with experimentation
Change the technical problem of corresponding laboratory apparatus, there is provided use for laboratory drosophila cultivates and doser.
The present invention is achieved by the following technical programs.
Use for laboratory drosophila cultivates and doser, and it includes blake bottle with being threadedly coupled mouth, the threaded connection mouth bag
Threaded connection mouth one is included with being threadedly coupled mouth two, wherein：The blake bottle includes epimere, stage casing and hypomere, epimere and hypomere
Upper surface is disposed as opening with lower surface, and the upper surface of hypomere is arranged to open, and the lower surface of hypomere is arranged to sealed end, on
Mouth one is connected through a screw thread between section and stage casing to connect, and mouth two is connected through a screw thread between stage casing and hypomere and is connected；
The upper end surface of the epimere is provided with bottle stopper, and decoction adding tube connects through bottle stopper with epimere, the side of decoction adding tube
Wall is provided with scale, and the sharp nozzle end end of decoction adding tube is plugged with cotton-wool, anesthetic air inlet is provided with the middle part of epimere side wall
One；
The bottom of the hypomere can add culture medium, and anesthesia gas air inlet two is provided with the middle part of hypomere side wall；
The side wall of the threaded connection mouth is provided with through hole, through hole is provided with rotating shaft, the external shaft end of rotating shaft is provided with hand
Handle, pointer is fixedly installed on the outer end of rotating shaft, positioned at pointer circular rotating track on the lateral wall of threaded connection mouth
O-clock position is provided with positive stop, and the o-clock position of pointer circular rotating track is located on the lateral wall in epimere and stage casing
Also it is provided with positive stop, handle drives baffle plate in the o-clock position of pointer circular rotating track to o'clock position by rotating shaft
Place rotates；
At threaded connection mouth one：The other end of rotating shaft is provided with sieve plate, and the lateral surface of sieve plate is with being threadedly coupled in mouth one
Wall is brought into close contact；
At threaded connection mouth two：The other end of rotating shaft is provided with baffle plate, and the outer rim of baffle plate is provided with sealing ring, sealing ring it is outer
Side is brought into close contact with the inwall for being threadedly coupled mouth two.
Further, the material of the sealing ring is silica gel.
Further, the anesthetic air inlet one and the anesthetic being passed through in anesthesia gas air inlet two are carbon dioxide gas
Body.
Further, the material of the epimere, stage casing and hypomere is transparent material, the material for the bottle stopper installed in epimere
For sponge or it is plastics.
Further, the material that the sieve plate is is rigid plastics, and mesh is provided with sieve plate, and mesh aperture is 1.5mm.
The present invention has the advantages that compared with prior art.
1st, blake bottle can use transparent lucite material, non-friable, avoid in experimentation because blake bottle crushes
The injury to caused by experimenter, while saved experimental cost.
2nd, reduce the number that drosophila is poured into different blake bottles, saved time cost for experimenter, reduced experiment
Flow.
3rd, check and verify and test for large batch of drosophila drug sieve, can largely reduce the quantity of required blake bottle, keep away
Exempt from excessive lab space to waste, while reduce the blake bottle quantity for needing to clean, save manpower and resource.
4th, it can ensure that drosophila is present in a device in experimentation, the experiment caused by drosophila that avoids repeatedly falling is slipped up.
5th, the device can effectively avoid during administration drosophila from flying out experimental provision, ensure the quantity of drosophila and the standard of experiment
True property.
6th, the device conveniently observes dosage and residual drug, beneficial to the amount of record drosophila intake medicine, more accurately divides
Analyse drug dose and effect.
7th, the device can give drosophila different medicines simultaneously, be easy to observe preference or detest of the drosophila to medicine, profit
Adjusted in the experimental design in later stage and medicine.
8th, carbon dioxide narcosis agent entrance is designed with, is easy to anaesthetize drosophila after the administration is complete, all drosophilas are poured into it
He carries out follow-up experiment by experimental provision.Avoid drosophila from flying out device, do not reach the drosophila quantity needed for experiment, influence experiment knot
Fruit.
In a word, the present apparatus provides a kind of drosophila culture and doser, solves in current drosophila administration experimentation
The problem of existing.
Brief description of the drawings
Fig. 1 is the overall structure diagram of drosophila cultivation stage in hypomere.
Fig. 2 is the overall structure diagram that drosophila is transferred to stage casing by hypomere.
The right side that Fig. 3 is Fig. 2 regards cross section structure diagram.
Fig. 4 is the overall structure diagram that drosophila stands the stage on an empty stomach in stage casing.
Fig. 5 is the overall structure diagram of drosophila dosed portions.
The right side that Fig. 6 is Fig. 5 regards cross section structure diagram.
Fig. 7 is partial enlargement structural representation when part A pointer coordinates with positive stop in Fig. 6.
In figure, 1 is decoction adding tube, and 2 be bottle stopper, and 3 be epimere, and 4 be anesthetic air inlet one, and 5 be sieve plate, and 6 be screw thread
Interface one, 7 be stage casing, and 8 be baffle plate, and 9 be sealing ring, and 10 be hickey two, and 11 be hypomere, and 12 be anesthesia gas air inlet two,
13 be through hole, and 14 be handle, and 15 be rotating shaft, and 16 be pointer, and 17 be positive stop, and 18 be culture medium.
Embodiment
The present invention is elaborated with reference to embodiment：The present embodiment is carried out premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
As shown in Figures 1 to 5, in the present embodiment use for laboratory drosophila culture and doser, it include blake bottle with
Mouth is threadedly coupled, the threaded connection mouth includes threaded connection mouth 1 with being threadedly coupled mouth 2 10, wherein：The blake bottle bag
Epimere 3, stage casing 7 and hypomere 11 are included, the upper surface and lower surface of epimere 3 and hypomere 11 are disposed as opening, the upper end of hypomere 11
Face is arranged to open, and the lower surface of hypomere 11 is arranged to sealed end, and the company of mouth 1 is connected through a screw thread between epimere 3 and stage casing 7
Connect, the connection of mouth 2 10 is connected through a screw thread between stage casing 7 and hypomere 11；
The upper end surface of the epimere 3 is provided with bottle stopper 2, and decoction adding tube 1 connects through bottle stopper 2 with epimere 3, decoction adding tube
1 side wall is provided with scale, from the upper end of decoction adding tube 1 by the sharp nozzle end end of cotton stopper to decoction adding tube 1, epimere 3
Anesthetic air inlet 1 is provided with the middle part of side wall；
The bottom of the hypomere 11 can add culture medium 18, and anesthesia gas air inlet 2 12 is provided with the middle part of the side wall of hypomere 11；
The side wall of the threaded connection mouth is provided with through hole 13, and through hole 13 is provided with rotating shaft 15, the external shaft end of rotating shaft 15
Handle 14 is provided with, pointer 16 is fixedly installed on the outer end of rotating shaft 15, is located at pointer on the lateral wall of threaded connection mouth
9 o-clock positions of 16 circular rotating tracks are provided with positive stop 17, and epimere 3 on the lateral wall in stage casing 7 with being located at pointer 16
12 o-clock positions of circular rotating track are also provided with positive stop 17, and handle 14 drives baffle plate 8 in pointer by rotating shaft 15
9 o-clock positions of 16 circular rotating tracks to 12 o-clock positions rotate；
At threaded connection mouth 1：The other end of rotating shaft 15 is provided with sieve plate 5, and the lateral surface of sieve plate 5 is with being threadedly coupled mouth one
6 inwall is brought into close contact；
At threaded connection mouth 2 10：The other end of rotating shaft 15 is provided with baffle plate 8, and the outer rim of baffle plate 8 is provided with sealing ring 9, close
The lateral surface of seal 9 is brought into close contact with the inwall for being threadedly coupled mouth 2 10.
Further, the material of the sealing ring 9 is silica gel.
Further, the anesthetic air inlet 1 and the anesthetic being passed through in anesthetic air inlet 2 12 are titanium dioxide
Carbon gas.
Further, the material of the epimere 3, stage casing 7 and hypomere 11 is transparent material, the bottle stopper 2 installed in epimere 3
Material be sponge or be plastics.
Further, the material that the sieve plate 5 is is rigid plastics, and mesh is provided with sieve plate 5, and mesh aperture is
1.5mm。
The use process of the present invention is as follows：
First, it is installed on as shown in figure 1, threaded connection mouth 2 10 is connected through a screw thread on the upper surface of hypomere 11, opens gear
Plate 8, Drosophila medium is put into hypomere 11, and drosophila is put into hypomere 11, closed baffle plate 8, the lower surface in stage casing 7 led to
The top that threaded connection is installed on threaded connection mouth 2 10 is crossed, epimere 3 is installed on to the upper end in stage casing 7 by hickey 1
The top in face, bottle stopper 2 is filled in into the upper surface open-mouth of epimere 3, sieve plate 5 is closed, mounted blake bottle is placed in drosophila and is adapted to
Growing environment in cultivate, treat subsequent experimental use；
Secondly, as shown in Figures 2 and 3, after drosophila feeding is abundant, CO is blown into hypomere 11 from anesthesia gas air inlet 2 122
Anesthetic gases.After drosophila is anaesthetized, blake bottle is inverted, opens baffle plate 8, drosophila in hypomere 11 by pouring into stage casing 7, closed baffle plate
8；
Again, as shown in figure 4, rotating and culturing bottle again, makes blake bottle just put, drosophila fully stands on an empty stomach in stage casing 7, together
When, decoction adding tube 1 is inserted in epimere 3 through bottle stopper 2, walks and uses after remaining；
Finally, as shown in Figures 5 to 7, carbon dioxide narcosis gas is passed through into anesthetic air inlet 1 and anesthesia gas air inlet two
12 are blown into blake bottle together, open sieve plate 5, and experimental drug is added dropwise into epimere 3 by the way that scale is quantitative with decoction adding tube 1,
Observed；Test each part disassembly, cleaning after terminating, remain next use.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Be familiar with those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (5)
1. use for laboratory drosophila cultivates and doser, it includes blake bottle includes with being threadedly coupled mouth, the threaded connection mouth
It is threadedly coupled mouth one（6）With being threadedly coupled mouth two（10）, it is characterised in that：The blake bottle includes epimere（3）, stage casing（7）With
Hypomere（11）, epimere（3）And stage casing（7）Upper surface and lower surface be disposed as opening, hypomere（11）Upper surface be arranged to
Opening, hypomere（11）Lower surface be arranged to sealed end, epimere（3）With stage casing（7）Between be connected through a screw thread mouth one（6）Even
Connect, stage casing（7）With hypomere（11）Between be connected through a screw thread mouth two（10）Connection；
The epimere（3）Upper end surface bottle stopper is installed（2）, decoction adding tube（1）Through bottle stopper（2）With epimere（3）Connection,
Decoction adding tube（1）Side wall be provided with scale, decoction adding tube（1）Sharp nozzle end end be plugged with cotton-wool, epimere（3）Side wall
Middle part is provided with anesthetic air inlet one（4）；
The hypomere（11）Bottom be pasted with culture medium（18）, hypomere（11）Anesthesia gas air inlet two is provided with the middle part of side wall
（12）；
The side wall of the threaded connection mouth is provided with through hole（13）, through through hole（13）It is provided with rotating shaft（15）, rotating shaft（15）'s
External shaft end is provided with handle（14）, rotating shaft（15）Outer end on be fixedly installed pointer（16）, in the outer of threaded connection mouth
It is located at pointer in side wall（16）9 o-clock positions of circular rotating track are provided with positive stop（17）, epimere（3）With stage casing
（7）Lateral wall on be located at pointer（16）12 o-clock positions of circular rotating track are also provided with positive stop（17）, handle
（14）Pass through rotating shaft（15）Drive baffle plate（8）In pointer（16）9 o-clock positions of circular rotating track are to 12 o-clock positions
Rotate；
In threaded connection mouth one（6）Place：Rotating shaft（15）The other end be provided with sieve plate（5）, sieve plate（5）Lateral surface with screw thread
Connector one（6）Inwall be brought into close contact；
In threaded connection mouth two（10）Place：Rotating shaft（15）The other end be provided with baffle plate（8）, baffle plate（8）Outer rim be provided with it is close
Seal（9）, sealing ring（9）Lateral surface with being threadedly coupled mouth two（10）Inwall be brought into close contact.
2. use for laboratory drosophila culture according to claim 1 and doser, it is characterised in that：The sealing ring（9）
Material be silica gel.
3. use for laboratory drosophila culture according to claim 1 and doser, it is characterised in that：The anesthetic air inlet
Mouth one（4）With anaesthetizing gas air inlet two（12）In the anesthetic that is passed through be carbon dioxide.
4. use for laboratory drosophila culture according to claim 1 and doser, it is characterised in that：The epimere（3）, in
Section（7）And hypomere（11）Material be transparent material, epimere（3）The bottle stopper of middle installation（2）Material be sponge or be modeling
Material.
5. use for laboratory drosophila culture according to claim 1 and doser, it is characterised in that：The sieve plate（5）'s
Material is rigid plastics, sieve plate（5）On be provided with mesh, mesh aperture is 1.5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711270574.0A CN107864932B (en) | 2017-12-05 | 2017-12-05 | Drosophila culture and drug administration device for laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711270574.0A CN107864932B (en) | 2017-12-05 | 2017-12-05 | Drosophila culture and drug administration device for laboratory |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107864932A true CN107864932A (en) | 2018-04-03 |
| CN107864932B CN107864932B (en) | 2023-09-05 |
Family
ID=61755261
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711270574.0A Active CN107864932B (en) | 2017-12-05 | 2017-12-05 | Drosophila culture and drug administration device for laboratory |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107864932B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111436400A (en) * | 2020-03-17 | 2020-07-24 | 南阳师范学院 | Laboratory fruit fly culture bottle interface device |
| CN114073241A (en) * | 2021-11-11 | 2022-02-22 | 青岛农业大学 | Observation device applied to parasitic wasps and manufacturing method thereof |
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| CN207544113U (en) * | 2017-12-05 | 2018-06-29 | 山西大医院 | A kind of use for laboratory drosophila culture and drug delivery device |
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| CN205266669U (en) * | 2016-01-11 | 2016-06-01 | 四川农业大学 | A erlenmeyer flask for raising fruit bat |
| CN205266668U (en) * | 2016-01-11 | 2016-06-01 | 四川农业大学 | A wide -necked bottle for fruit bat is cultivateed |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111436400A (en) * | 2020-03-17 | 2020-07-24 | 南阳师范学院 | Laboratory fruit fly culture bottle interface device |
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| CN114073241B (en) * | 2021-11-11 | 2023-02-07 | 青岛农业大学 | Observation device applied to parasitic wasps and manufacturing method thereof |
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| Publication number | Publication date |
|---|---|
| CN107864932B (en) | 2023-09-05 |
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Address after: No.99 Longcheng street, Taiyuan City, Shanxi Province Applicant after: Shanxi Bethune hospital (Shanxi Academy of Medical Sciences Shanxi Hospital of Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of science and technology the Third Hospital of Shanxi Medical University and the Third Clinical Medical College of Shanxi Medical University) Address before: No.99 Longcheng street, Taiyuan City, Shanxi Province Applicant before: SHANXI DAYI Hospital |
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