CN114073241B - Observation device applied to parasitic wasps and manufacturing method thereof - Google Patents
Observation device applied to parasitic wasps and manufacturing method thereof Download PDFInfo
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- CN114073241B CN114073241B CN202111331697.7A CN202111331697A CN114073241B CN 114073241 B CN114073241 B CN 114073241B CN 202111331697 A CN202111331697 A CN 202111331697A CN 114073241 B CN114073241 B CN 114073241B
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- 241001481304 Vespoidea Species 0.000 title claims abstract description 35
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 229920003023 plastic Polymers 0.000 claims abstract description 85
- 230000003071 parasitic effect Effects 0.000 claims abstract description 65
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000004033 plastic Substances 0.000 claims abstract description 48
- 239000002985 plastic film Substances 0.000 claims abstract description 30
- 229910052742 iron Inorganic materials 0.000 claims abstract description 24
- 230000006399 behavior Effects 0.000 claims abstract description 21
- 239000002023 wood Substances 0.000 claims abstract description 16
- 230000000149 penetrating effect Effects 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims description 13
- 239000000853 adhesive Substances 0.000 claims description 10
- 230000001070 adhesive effect Effects 0.000 claims description 10
- 241000257303 Hymenoptera Species 0.000 claims description 8
- 239000012790 adhesive layer Substances 0.000 claims description 4
- 241000382353 Pupa Species 0.000 abstract description 11
- 239000003292 glue Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000255601 Drosophila melanogaster Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 230000024241 parasitism Effects 0.000 description 3
- 241000255925 Diptera Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000007270 Gaultheria hispida Nutrition 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 235000009134 Myrica cerifera Nutrition 0.000 description 1
- 244000024215 Myrica gale Species 0.000 description 1
- 235000012851 Myrica pensylvanica Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 1
- 240000000851 Vaccinium corymbosum Species 0.000 description 1
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021014 blueberries Nutrition 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- YGANSGVIUGARFR-UHFFFAOYSA-N dipotassium dioxosilane oxo(oxoalumanyloxy)alumane oxygen(2-) Chemical compound [O--].[K+].[K+].O=[Si]=O.O=[Al]O[Al]=O YGANSGVIUGARFR-UHFFFAOYSA-N 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 229910052627 muscovite Inorganic materials 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses an observation device applied to parasitic wasps and a manufacturing method thereof, which are used for observing parasitic behaviors of the parasitic wasps in an all-around and multi-angle manner. The observation device comprises a transparent plastic box, a gauze fixedly arranged at the bottom of the transparent plastic box, a first through hole penetrating and arranged at the central position of the gauze, a plastic box cover movably arranged at the box opening of the transparent plastic box, a second through hole penetrating and arranged at the central position of the plastic box cover and a round wood rod slidably arranged in the transparent plastic box. The plastic box cover is connected with an iron nail through threads, and a round plastic sheet is movably arranged on the plastic box cover through the iron nail. The parasitic position and angle of the parasitic wasp on the host pupa can be clearly observed, the omnibearing and multi-angle observation is realized, the convenience is provided for researching and understanding the parasitic behavior rule of the parasitic wasp, in addition, the device is convenient to assemble and operate, the manufacturing cost is low, and the device is suitable for wide popularization and use.
Description
Technical Field
The invention relates to the technical field of parasitic behavior observation of parasitic wasps, in particular to an observation device applied to parasitic wasps and a manufacturing method of the observation device.
Background
The drosophila melanogaster belongs to the order diptera and is an important pest of various fruits such as waxberries, cherries, peaches, pears, apples, grapes, blueberries, strawberries, apricots and the like. The fly pupa ceratophaga is a parasitism bee outside the pupal stage of various diptera pests, and when parasitizing, eggs are usually laid between pupal shells and pupal bodies of hosts to paralyze the hosts to die, so that the parasitism bee is an inhibitory parasitism bee. Based on the method, the use of the golden wasp pupae for biological control of the fruit fly pests has good application prospect, and the observation of the parasitic behavior of the parasitic wasps is an important precondition for biological control by using the parasitic wasp.
However, the observation of parasitic behavior of parasitic bees presents the following difficulties: the parasitic wasps and the hosts often move and are not fixed in the parasitic behavior generation process, which can interfere the observation sight, hinder the observation and bring inconvenience to the study of the parasitic behavior.
Disclosure of Invention
Therefore, it is necessary to provide an observation device applied to parasitic wasps and a manufacturing method thereof, aiming at the problems that the positions and angles of the parasitic wasps parasitizing the hosts are variable, the positions of the parasitic wasps and the hosts are often not fixed in the process of occurrence of parasitic behaviors, and the positions interfere with the observation sight, hinder observation, and bring inconvenience to study the parasitic behaviors.
An observation device applied to parasitic wasps is used for observing parasitic behaviors of the parasitic wasps in an omnibearing and multi-angle mode, and comprises a transparent plastic box, a gauze fixedly installed at the bottom of the transparent plastic box, a first through hole penetrating through the central position of the gauze, a plastic box cover movably installed at the box opening of the transparent plastic box, a second through hole penetrating through the central position of the plastic box cover and a log rod slidably installed in the transparent plastic box;
the plastic box cover is in threaded connection with an iron nail, and a round plastic sheet is movably mounted on the plastic box cover through the iron nail; the box bottom and the box opening of the transparent plastic box are of a through structure and are used for providing a parasitic space for the parasitic wasps; the round plastic sheet is arranged at the position of the plastic box cover back to the transparent plastic box and is rotationally arranged by taking the iron nail as a shaft;
the end part of the log rod facing the plastic box cover is coated with an adhesion layer which is used for fixing host pupae parasitized by the parasitizing bees.
The observation device can be obtained by simple splicing, the device is easy to obtain in materials, convenient to assemble, low in cost and good in reusability, parasitic parts and angles of parasitic bees on host pupae can be clearly observed, and all-dimensional and multi-angle observation is realized.
In one embodiment, the circular plastic sheet and the transparent plastic case have the same diameter size.
In one embodiment, the adhesive layer is made of fixing adhesive or double-sided adhesive.
In one embodiment, the round wood rod is connected between the gauze and the plastic box cover through the first through hole and the second through hole in a sliding penetrating mode.
Further, the diameter of the first through hole is 3mm, and the diameter of the second through hole is 4mm; the round wood rod has the following specifications: length 80mm, diameter 2mm.
A method for manufacturing an observation device, which is used for manufacturing the observation device applied to parasitic wasps, the method for manufacturing the observation device comprises the following steps:
s1, providing a transparent plastic box, a plastic box cover, a round wood rod, an iron nail, a round plastic sheet and a gauze;
the center of the plastic box cover is provided with a through hole; one end of the round wood bar is coated with a fixing adhesive or a double-sided adhesive;
s2, removing the bottom of the transparent plastic box to obtain a through box;
s3, adhering the gauze to the box bottom of the box, and forming a hole in the center of the gauze;
and S4, nailing the round plastic sheet on the plastic box cover by using the iron nails.
In one embodiment, the through hole on the plastic box cover and the opening hole on the gauze provide the assembly positioning and moving channel of the round wooden stick.
In one embodiment, the round plastic sheet is attached to the plastic box cover; when the round plastic sheet is pulled, the round plastic sheet rotates by taking the iron nail as a shaft.
Compared with the prior art, the invention has the beneficial effects that:
the observation device can be obtained by simple splicing, the materials of the device are easy to obtain, the device is convenient and fast to assemble, the cost is low, the reusability is good, and a parasitic space of a fixed area is provided, so that the parasitic position and the parasitic angle of parasitic wasps on host pupae can be clearly observed, and the omnibearing and multi-angle observation is realized.
The observation device can carry out all-around and multi-angle observation, provides convenience for researching and understanding the parasitic behavior rule of parasitic wasps, and provides experiment and data support for the research of biological control by using the parasitic wasps.
The manufacturing method of the invention has convenient assembly operation and low manufacturing cost, and is suitable for wide popularization and use.
Drawings
Fig. 1 is a schematic structural diagram of an observation device applied to a parasitic bee according to embodiment 1 of the present invention.
Fig. 2 is a perspective view of the invention shown in fig. 1.
Description of the main elements
1. A transparent plastic case; 2. a first through hole; 3. round wooden sticks; 4. iron nails; 5. a round plastic sheet; 6. a second through hole; 7. a plastic box cover; 8. a gauze.
The invention is further described in detail by the main reference symbols in the above description and the accompanying drawings.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings.
Example 1
Referring to fig. 1-2, the present embodiment provides an observation device for parasitic wasps, which is used for observing parasitic behaviors of the parasitic wasps in an all-around and multi-angle manner. The observation device comprises a transparent plastic box 1, a gauze 8 fixedly arranged at the bottom of the transparent plastic box 1, a first through hole 2 penetrating through the central position of the gauze 8, a plastic box cover 7 movably arranged at the box opening of the transparent plastic box 1, a second through hole 6 penetrating through the central position of the plastic box cover 7 and a log rod 3 slidably arranged in the transparent plastic box 1.
The bottom and the opening of the transparent plastic box 1 are of a through structure and are used for providing a parasitic space for parasitic bees. The end of the round stick 3 facing the plastic box cover 7 is coated with an adhesive layer for fixing the host pupae parasitized by the parasitizing bees. The adhesion layer adopts fixing glue or double-sided glue. In the embodiment, the transparent plastic box 1, the gauze 8 and the plastic box cover 7 form the observation device which can be simply spliced and can be obtained, the parasitic behavior observation space of the fixed area is provided, the observation device is easy to obtain materials, convenient and fast to assemble, low in cost and good in reusability, and the parasitic position and the parasitic angle of the parasitic wasp on the host pupa can be clearly observed by providing the parasitic space of the fixed area, so that the omnibearing and multi-angle observation is realized.
The round wooden rod 3 is connected between the gauze 8 and the plastic box cover 7 in a sliding and penetrating mode through the first through hole 2 and the second through hole 6. The diameter of the first through-hole 2 is 3mm, and the diameter of the second through-hole 6 is 4mm. The round stick 3 has the following specifications: length 80mm, diameter 2mm. The first through hole 2 and the second through hole 6 are matched to provide a use passage of the round wood rod 3. Referring to fig. 2, an arrow in fig. 2 indicates the insertion direction of the log rod 3. The round wood rod 3 provides parasitic support of the parasitic peak on the host pupa, and after the parasitic action is finished, the round wood rod 3 penetrates out along the second through hole 6, so that the parasitic bee and other parasitic peaks of the selected host can be separated, and the interference of other parasitic bees is prevented, and the parasitic process is interrupted.
The plastic box cover 7 is connected with an iron nail 4 in a threaded manner, and the plastic box cover 7 is movably provided with a round plastic sheet 5 through the iron nail 4. The round plastic sheet 5 is arranged at the position of the plastic box cover 7 back to the transparent plastic box 1, and the round plastic sheet 5 is arranged by rotating by taking the iron nail 4 as a shaft. The round plastic sheet 5 and the transparent plastic box 1 have the same diameter. The gauze 8 is used for observing the circulation of air in space to combine through the plastics lid 7 of installing by iron nail 4 when parasitic bee is carried out parasitizing in observing the space, prevent that parasitic bee from running out, ensure the smoothness nature of parasitic action observation. When the parasitic wasp with the selected host needs to be taken out, the round plastic sheet 5 is stirred, the round plastic sheet 5 rotates along the iron nail 4 until the second through hole 6 is exposed, and at the moment, the round wood rod 3 can be normally pushed out.
To sum up, the observation device of this embodiment can obtain through simple and easy concatenation, and the device material is easily obtained, and the equipment is convenient, and low cost and reusability are good, but clear observation parasitic bee is at parasitic position and angle on host pupa, realizes all-round and multi-angle's observation.
The present embodiment further provides an observation method of the observation device, which operates as follows:
the plastic box cover 7 is opened, a plurality of parasitic wasps are placed in the transparent plastic box 1, then the plastic box cover 7 is closed, the host pupa is placed at one end of the round wood rod 3 and is adhered and fixed through the solid glue/double-faced glue, one end of the round wood rod 3, which is fixed with the host pupa, is inserted into the transparent plastic box 1 from the first through hole 2, and a researcher observes parasitic behaviors of the parasitic wasps in the transparent plastic box 1.
After the parasitic wasp selects the host pupa, the round plastic sheet 5 is stirred, at the moment, the round plastic sheet 5 rotates by taking the iron nail 4 as a shaft until the plastic box cover 7 is exposed, the round wood rod 3 together with the host pupa and the parasitic wasp are pushed out of the observation cavity along the second through hole 6, and the parasitic behavior of the parasitic wasp is continuously observed and recorded under a meat eye/microscope. In the embodiment, the parasitic wasps of the selected hosts are timely released, so that the interference of other parasitic wasps is prevented, the parasitic process is interrupted, and the normal operation of observation and research is guaranteed.
Since drosophila melanogaster has the characteristics of easy propagation, low cost, mature propagation technology, transparent pupa body, easy observation and suitability for observing parasitic behaviors, in the embodiment, 2 days old drosophila melanogaster pupae is selected as host pupae, 4 female muscovite pupae which are eclosion for 24 hours and fully mate are selected as parasitic wasps to carry out parasitic test, and the observed experimental results are recorded as shown in table 1.
TABLE 1
Table 1 shows the stop position records of the actions of tail knocking and detecting of the parasitic wasp on the host pupa, knocking and detecting of the ovipositor, inserting and stopping of the ovipositor, taking out the ovipositor for taking food, inserting and stopping of the original position of the ovipositor and searching of the ovipositor, and the time taken record for completing the parasitic actions. The experimental results in table 1 demonstrate that the observation device of this embodiment can carry out the observation of all-round and multi-angle, for the parasitic behavior law of research and understanding parasitic wasp facilitates, for the research that utilizes parasitic wasp to carry out biological control provides experiment and data support. The observation method of the embodiment provides convenience for obtaining the parasitic behavior rule of the parasitic wasps by observing and recording the parasitic behaviors of the parasitic wasps in all directions and at multiple angles, and is simple, effective and convenient to operate.
Example 2
This embodiment provides a method for manufacturing an observation device, which is used in manufacturing the observation device applied to parasitic wasps as in embodiment 1. The manufacturing method comprises the following steps:
s1 provides a transparent plastic box 1, a plastic box cover 7, a round wood rod 3, iron nails 4, a round plastic sheet 5 and a gauze 8.
The center of the plastic box cover 7 is provided with a through hole, and the plastic box cover 7 is movably arranged at the box opening of the transparent plastic box 1. One end of the round stick 3 is coated with a fixing adhesive or a double-sided adhesive for fixing the host pupae.
And S2, removing the box bottom of the transparent plastic box 1 to obtain a through box.
S3, the gauze 8 is adhered to the box bottom of the box, and a hole is formed in the center of the gauze 8.
The through hole on the plastic box cover 7 and the opening on the gauze 8 provide the assembly positioning and moving channel of the round wooden rod 3.
And S4, nailing the round plastic sheet 5 on the plastic box cover 7 by using the iron nails 4.
The round plastic sheet 5 is attached to the plastic box cover 7 and manually rotated by using the iron nail 4 as an axis, so that parasitic bees are prevented from flying out of the through hole in the plastic box cover 7 in the parasitic behavior observation process.
The manufacturing method of the embodiment is convenient to assemble and operate, low in manufacturing cost and suitable for wide popularization and use.
For the naming of each component referred to, the function described in the specification is used as the standard for naming, but is not limited by the specific term used in the present invention, and those skilled in the art can also select other terms to describe each component name of the present invention.
Claims (8)
1. An observation device applied to parasitic wasps is used for observing parasitic behaviors of the parasitic wasps in an omnibearing and multi-angle manner, and is characterized by comprising a transparent plastic box (1), a gauze (8) fixedly installed at the bottom of the transparent plastic box (1), a first through hole (2) penetrating through the central position of the gauze (8), a plastic box cover (7) movably installed at the box opening of the transparent plastic box (1), a second through hole (6) penetrating through the central position of the plastic box cover (7) and a log rod (3) slidably installed in the transparent plastic box (1);
wherein the plastic box cover (7) is connected with an iron nail (4) in a threaded manner, and the plastic box cover (7) is movably provided with a round plastic sheet (5) through the iron nail (4); the box bottom and the box opening of the transparent plastic box (1) are of a through structure and are used for providing a parasitic space for parasitic bees; the round plastic sheet (5) is arranged at the position of the plastic box cover (7) back to the transparent plastic box (1), and the round plastic sheet (5) is rotationally arranged by taking the iron nail (4) as a shaft;
the end part of the round wooden rod (3) facing the plastic box cover (7) is coated with an adhesive layer which is used for fixing host pupae parasitized by the parasitic wasps.
2. Observation device applied to parasitic wasps according to claim 1, characterized in that said circular plastic sheet (5) and said transparent plastic box (1) have a diameter of the same size.
3. The observation device applied to the parasitic wasp according to claim 1, wherein the adhesive layer is made of a fixing adhesive or a double-sided adhesive.
4. An observation device applied to parasitic wasps according to claim 1, wherein the round wooden rod (3) is slidably connected between the gauze (8) and the plastic box cover (7) through the first through hole (2) and the second through hole (6).
5. Observation device applied to parasitic wasps according to claim 4, characterized in that said first through hole (2) has a diameter of 3mm and said second through hole (6) has a diameter of 4mm;
the specification of the round wood rod (3) is as follows: length 80mm, diameter 2mm.
6. A method for manufacturing an observation device for parasitic wasps according to any one of claims 1 to 5, comprising the steps of:
s1, providing a transparent plastic box (1), a plastic box cover (7), a round wood rod (3), iron nails (4), a round plastic sheet (5) and a gauze (8);
a through hole is formed in the center of the plastic box cover (7); one end of the round wood rod (3) is coated with a fixing adhesive or a double-sided adhesive;
s2, removing the box bottom of the transparent plastic box (1) to obtain a through box;
s3, adhering the gauze (8) to the box bottom of the box, and forming a hole in the center of the gauze (8);
and S4, nailing the round plastic sheet (5) on the plastic box cover (7) by using the iron nails (4).
7. A method for making a scope according to claim 6, wherein the through holes in the plastic cover (7) and the openings in the screen (8) provide access for positioning and moving the log rod (3).
8. A method of making an observation device according to claim 6, wherein the round plastic sheet (5) is attached to the plastic cover (7);
when the round plastic sheet (5) is pulled, the round plastic sheet rotates by taking the iron nail (4) as an axis.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111331697.7A CN114073241B (en) | 2021-11-11 | 2021-11-11 | Observation device applied to parasitic wasps and manufacturing method thereof |
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| CN202111331697.7A CN114073241B (en) | 2021-11-11 | 2021-11-11 | Observation device applied to parasitic wasps and manufacturing method thereof |
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| CN114073241B true CN114073241B (en) | 2023-02-07 |
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| CN114073241A (en) | 2022-02-22 |
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