CN107711726A - A kind of egg parasitoidses raising collection device and collection method - Google Patents

A kind of egg parasitoidses raising collection device and collection method Download PDF

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Publication number
CN107711726A
CN107711726A CN201711233953.2A CN201711233953A CN107711726A CN 107711726 A CN107711726 A CN 107711726A CN 201711233953 A CN201711233953 A CN 201711233953A CN 107711726 A CN107711726 A CN 107711726A
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CN
China
Prior art keywords
raising
ovum
fixed part
tea
collection device
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Pending
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CN201711233953.2A
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Chinese (zh)
Inventor
李金玉
尤民生
韩李伟
施龙清
白建林
何海芳
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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Priority to CN201711233953.2A priority Critical patent/CN107711726A/en
Publication of CN107711726A publication Critical patent/CN107711726A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Food-Manufacturing Devices (AREA)

Abstract

The present invention relates to a kind of egg parasitoidses raising collection device and collection method, the device includes setting culture matrix, keeping portion upper end open in keeping portion, the fixed part positioned at middle part and the superposed raising portion of bottom, keeping portion;Fixing hole is set in the middle part of fixed part, and fixed part upper surface sets detent one, lower surface to set detent two;Raising portion is provided with provisions hole and passage, raises subordinate's end opening.This raising collection device is simple in construction, is easy to load and unload, cost is cheap, easy to operate, realizes the raising and collection of indoor egg parasitoidses.

Description

A kind of egg parasitoidses raising collection device and collection method
Technical field
This patent is related to a kind of egg parasitoidses raising collection device and collection method.
Background technology
Tea lesser leafhopper is one of most important insect of tea tree, and in the world, Main Tea leaf plantation country is distributed, every year 15-20% economic loss is brought to global Tea Production.Tealeaves is the health being becoming increasingly popular in a kind of world wide Drink, as the tea lesser leafhopper of tea place primary pest, chemical pesticide is still depended in its preventing and treating, which unduly, causes tealeaves agriculture residual exceeded, directly Connect the harm person's of drinking tea health, thus there is an urgent need to safely, effectively, sustainable replacement control prece.Tassel chalcid fly is that tea is small The main egg parasitoidses of greenery cicada, two kinds of tassel chalcid flies are related generally to, be three rod tassel Nikols respectivelyStethyniumSp. and split Bone tassel NikolSchizophragmaSp species.They are made ovum to hatch, subtracted by the ovum of parasitic tea lesser leafhopper The quantity of tea lesser leafhopper is lacked, certain control action is played to tea lesser leafhopper population.Field investigation result shows that tassel is small Honeybee tea place parasitic rate more than 50%, only it is noted that Sustainable use, certain natural contral is might have to tea lesser leafhopper and is made With so as to reduce or even avoid the use of chemical pesticide during tea lesser leafhopper is prevented and treated, the safety of raising tea place production Property.Therefore, it is both tassel chalcid flies of more preferable Sustainable use, the enquiry based work of tassel chalcid fly biology and ecological characteristics just shows Obtain especially important.
Want the biology and ecological characteristics of investigation tassel chalcid fly, it is necessary first to obtain sufficient amount of tassel chalcid fly. Tassel chalcid fly build is small, and body is about 0.5 mm, in tea place only be visually not easy observe.It is difficult by free-hand or conventional tool to want To capture sufficient amount of tassel chalcid fly.Under study for action, adopt the tip more and take back interior, solution takes smaller green leaf hopper under the microscope in the past Ovum, after put culture dish moisturizing culture wait the small bee eclosion of tassel go out ovum method collect needed for experiment material, technical difficulty is big, takes When it is laborious.
The content of the invention
In view of the deficiencies of the prior art, the technical problems to be solved by the invention are to provide a kind of egg parasitoidses raising Collection device and collection method, are not only simple in structure, and are easy to load and unload, and cost is cheap, and easy to operate, can realize indoors A large amount of, efficient and convenient collection to egg parasitoidses.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme:A kind of egg parasitoidses raise collection device, It includes setting culture matrix in portion positioned at the keeping portion of bottom, the fixed part positioned at middle part and superposed raising portion, keeping, Keeping portion upper end open;Fixing hole is set in the middle part of fixed part, and fixed part upper surface sets detent one, lower surface to set positioning Groove two;Raising portion is provided with provisions hole and passage, raises subordinate's end opening.
Further, passage is evenly and symmetrically arranged in the middle part of raising portion in the horizontal direction, and is sealed using gauze.
Further, provisions hole is arranged at the top of raising portion.
Further, the cotton with hydromel is set in provisions hole.
Further, keeping portion is tubbiness, and fixed part is tabular, and raising portion is spherical crown shape, and the circular section of spherical crown is down.
Further, keeping portion and raising portion use transparent plastics material.
Preferably, the height in the keeping portion is 8-15cm, and upper end open external diameter is 8-15cm;The a diameter of 3-4 of fixing hole cm;The height in the raising portion is 9-16cm, and lower ending opening external diameter is 6-9cm, and provisions bore dia is 1cm, and passage is a diameter of 3-4cm, the aperture 80-100 mesh of gauze.
Further, the collection method of egg parasitoidses raising collection device, comprises the following steps:
Step S1:Fresh band ovum tea branch is taken, band ovum tea every tealeaves of branch is carried out to cut the processing of half leaf;
Step S2:Culture matrix is poured into keeping portion, the more plants of band ovum tea branches handled well through cutting half leaf are taken, by with ovum tea branch Bottom limb is wound with winding cotton, and winding the position of cotton will be such that band ovum tea branch lower length is mutually fitted with keeping portion height Answer, be highly adapted with ovum tea branch upper length and raising portion, the thickness for winding cotton also will be with the fixing hole in the middle part of fixed part It is adapted, winding cotton position is determined and the fixing hole of fixed part is filled in after winding, and fixed part is placed in keeping portion, is protected Card band ovum tea branch bottom is immersed to maintain band ovum tea branch growth in culture matrix, and keeping portion tip edge is inserted into determining for fixed part In the groove two of position, fixed part is set to be fixed on keeping portion;
Step S3:Raising portion is placed on fixed part, makes the detent of the edge insertion fixed part of the lower openings in raising portion In one, to ensure that raising portion is fixed on fixed part, the band leaf site with ovum tea branch is covered;
Step S4:Cotton with hydromel is filled in provisions hole to supplement food needed for egg parasitoidses;
Step S5:Culture matrix old in keeping portion is outwelled at regular intervals, changes fresh culture matrix to meet band ovum Needed for tea branch growth, at the same time, raising portion is periodically gently opened, collects the egg parasitoidses sprouted wings in raising portion inwall; Collection work continues until that band ovum tea branch is withered.
Further, the limb of 6-9cm or so under the spray and spray of slightly five leaves is remained with ovum tea branch;Take ten plants of bands Ovum tea branch;Culture matrix was changed every three days;Raising portion is opened daily is collected work.
Further, while egg parasitoidses are collected, collected at the tealeaves back side hatched tea lesser leafhopper nymph and Adult.
Compared with prior art, the present invention has following remarkable advantage:This raising collection device is simple in construction, is easy to fill Unload, cost is cheap, easy to operate, can realize raising and collection to egg parasitoidses indoors;Keeping portion provides nutrient and water Divide normal emergence naturally, the development for maintain needed for the survival of insert band ovum tea branch and growth, farthest ensureing egg parasitoidses Needed for subsequent growth extra-nutrition;Raising portion provides space needed for the branches and leaves stretching, extension of band ovum and egg parasitoidses are sprouted wings and movable institute Condition is needed, because parasitic wasp has the habit upwards and to photo-reactive, the parasitic wasp after generally sprouting wings is concentrated in raising spheroid On wall, and it is transparent plastics material to raise ball material, the egg parasitoidses being thus easily observe that after sprouting wings, also causes parasitism The collection work of honeybee is very convenient.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the embodiment of the present invention.
In figure:1- keepings portion, 2- fixed parts, 3- raisings portion, 4- culture matrixes, 5- provisions hole, cottons of the 6- with hydromel, 7- passages, 8- gauzes, 9- fixing holes, 10- detents one, 11- detents two, 12- band ovum tea branches, 13- winding cottons.
Embodiment
The present invention will be further described in detail with reference to the accompanying drawings and detailed description.
As shown in figure 1, the present invention provides a kind of egg parasitoidses raising collection device, it is characterised in that:The device is by supporting Portion 1, fixed part 2 and the three parts of raising portion 3 composition.Keeping portion provides nutrient and moisture ensures the survival of insert band ovum tea branch and growth It is required, raising portion provide space and egg parasitoidses needed for band ovum branches and leaves stretching, extension sprout wings and activity needed for condition, fixed position in Between keeping portion and raising portion, for fixing and supporting tea branch egg parasitoidses.
The keeping portion is tubbiness, is surrounded by side wall and bottom, and upper end is open.Keeping portion inner bottom part is filled with culture Matrix 4, for cultivating band ovum tea branch.The keeping portion uses high transparency plastic material, and the height in the keeping portion is 8-15cm, Upper end open external diameter is 8-15cm.
The raising portion is spherical crown shape, and down, lower end is open for the circular section of raising portion spherical crown.The raising portion offers one Individual provisions hole 5(The cm of Circularhole diameter 1), provisions hole 5 is stoppered using the cotton 6 with hydromel.In the middle part of the raising portion it is symmetrical and 4 passages 7 are uniformly arranged in the horizontal direction(Circularhole diameter 3-4 cm), with gauze 8(Aperture 80-100 mesh)Sealing, is used for The ventilation in raising portion.The raising portion uses high transparency plastic material, and the height in the raising portion is 9-16cm, outside lower ending opening Footpath is 6-9cm.
The fixed part is tabular, and middle part offers fixing hole 9, and upper surface offers the detent 1 of circle, under Surface offers detent 2 11, and the diameter of the detent one is identical with raising portion lower openings external diameter, the positioning The diameter of groove two is identical with keeping portion upper end open external diameter, the fixed part by being connected on detent one with raising portion, It is connected by detent with keeping portion for two times, the fixing hole(Diameter 3-4 cm)For placing band ovum tea branch 12, the band Ovum tea branch is fixed by winding cotton 13 and the fixing hole and its underpart is soaked in culture matrix.
The present invention also provides a kind of collection method of egg parasitoidses raising collection device, comprises the following steps:
Step S1:Fresh band ovum tea branch 12 is taken, it is left to ensure to remain with 6-9cm under the spray and spray of slightly five leaves with ovum tea branch 12 Right limb, band ovum tea every leaf of branch is carried out to cut the processing of half leaf;
Step S2:Culture matrix 4 is poured into keeping portion 2, takes 10 plants of band ovum tea branches handled well through cutting half leaf, will be with ovum tea branch Limb is wound with winding cotton 13 below one slightly five leaves, and band ovum tea branch lower length will be made with supporting by winding the position of cotton Portion is highly adapted, band ovum tea branch upper length and raising portion 3 are highly adapted, and the thickness for winding cotton also will be with fixed part 3 The fixing hole 9 at middle part is adapted, and winding cotton position is determined and the fixing hole of fixed part is filled in after winding, and fixed part is put In in keeping portion, ensureing to immerse in culture matrix with maintenance with ovum tea branch growth with ovum tea branch bottom, by keeping portion tip edge Insert in fixed part detent 2 11, make fixed part is seamless to be fixed on keeping portion;
Step S3:Raising portion is placed on fixed part, makes the detent of the edge insertion fixed part of the lower openings in raising portion In one 10, it is fixed on ensureing that raising portion is seamless on fixed part, covers the position with slightly five leaves of ovum tea branch one;
Step S4:Cotton with hydromel is filled in provisions hole 5 to supplement food needed for parasitic wasp;
Step S5:Culture matrix old in keeping portion was outwelled every three days, changes fresh culture matrix to meet band ovum tea branch Needed for growth, at the same time, raising portion is gently opened daily, parasitic wasp because of the characteristics of having light and up flight and usually can be Movable habit on raising portion inwall, the egg parasitoidses sprouted wings can be collected in raising portion inwall, and according to smaller green leaf hopper Like hiding and suck juice at the tealeaves back side, hatched tea lesser leafhopper nymph and adult can be collected at the tealeaves back side in passing. Collection work can continue until that tea branch is withered, and usual tea branch can survive more than 20 days.
In summary, this raising collection device is simple in construction, is easy to load and unload, cost is cheap, easy to operate, can be in room Raising and collection of the interior realization to egg parasitoidses.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, it should all belong to the covering scope of the present invention.

Claims (10)

1. a kind of egg parasitoidses raise collection device, it is characterised in that:Including positioned at the keeping portion of bottom, the fixation positioned at middle part Portion and superposed raising portion, culture matrix, keeping portion upper end open are set in keeping portion;Fixing hole is set in the middle part of fixed part, Fixed part upper surface sets detent one, lower surface to set detent two;Raising portion is provided with provisions hole and passage, raises Support subordinate's end opening.
2. egg parasitoidses according to claim 1 raise collection device, it is characterised in that:Passage is uniform in the horizontal direction And be symmetricly set in the middle part of raising portion, and sealed using gauze.
3. egg parasitoidses according to claim 1 or 2 raise collection device, it is characterised in that:Provisions hole is arranged on raising At the top of portion.
4. egg parasitoidses according to claim 3 raise collection device, it is characterised in that:Band hydromel is set in provisions hole Cotton.
5. the egg parasitoidses raising collection device according to claim 1 or 2 or 4, it is characterised in that:Keeping portion is tubbiness, Fixed part is tabular, and raising portion is spherical crown shape, and the circular section of spherical crown is down.
6. the egg parasitoidses raising collection device according to claim 1 or 2 or 4, it is characterised in that:Keeping portion and raising portion Use transparent plastics material.
7. the egg parasitoidses raising collection device according to claim 1 or 2 or 4, it is characterised in that:The height in the keeping portion It is 8-15cm to spend for 8-15cm, upper end open external diameter;The a diameter of 3-4 cm of fixing hole;The height in the raising portion is 9-16cm, Lower ending opening external diameter is 6-9cm, and provisions bore dia is 1cm, a diameter of 3-4cm of passage, the aperture 80-100 mesh of gauze.
8. the collection method of the egg parasitoidses raising collection device according to one of claim 1-7, it is characterised in that:Including Following steps:
Step S1:Fresh band ovum tea branch is taken, band ovum tea every tealeaves of branch is carried out to cut the processing of half leaf;
Step S2:Culture matrix is poured into keeping portion, the more plants of band ovum tea branches handled well through cutting half leaf are taken, by with ovum tea branch Bottom limb is wound with winding cotton, and winding the position of cotton will be such that band ovum tea branch lower length is mutually fitted with keeping portion height Answer, be highly adapted with ovum tea branch upper length and raising portion, the thickness for winding cotton also will be with the fixing hole in the middle part of fixed part It is adapted, winding cotton position is determined and the fixing hole of fixed part is filled in after winding, and fixed part is placed in keeping portion, is protected Card band ovum tea branch bottom is immersed to maintain band ovum tea branch growth in culture matrix, and keeping portion tip edge is inserted into determining for fixed part In the groove two of position, fixed part is set to be fixed on keeping portion;
Step S3:Raising portion is placed on fixed part, makes the detent of the edge insertion fixed part of the lower openings in raising portion In one, to ensure that raising portion is fixed on fixed part, the band leaf site with ovum tea branch is covered;
Step S4:Cotton with hydromel is filled in provisions hole to supplement food needed for egg parasitoidses;
Step S5:Culture matrix old in keeping portion is outwelled at regular intervals, changes fresh culture matrix to meet band ovum Needed for tea branch growth, at the same time, raising portion is periodically gently opened, collects the egg parasitoidses sprouted wings in raising portion inwall; Collection work continues until that band ovum tea branch is withered.
9. the collection method of egg parasitoidses raising collection device according to claim 8, it is characterised in that:Band ovum tea branch is protected Leave the limb of 6-9cm or so under the spray and spray of slightly five leaves;Take ten plants of band ovum tea branches;Culture medium was changed every three days Matter;Raising portion is opened daily is collected work.
10. the collection method of egg parasitoidses raising collection device according to claim 9, it is characterised in that:Collecting ovum While parasitic wasp, hatched tea lesser leafhopper nymph and adult are collected at the tealeaves back side.
CN201711233953.2A 2017-11-30 2017-11-30 A kind of egg parasitoidses raising collection device and collection method Pending CN107711726A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108174827A (en) * 2018-02-27 2018-06-19 福建农林大学 A kind of Liriomyza raising collection device and its application method
CN108293965A (en) * 2018-03-29 2018-07-20 福建省农业科学院植物保护研究所 A kind of method of field acquisition tea lesser leafhopper
CN111838077A (en) * 2019-04-28 2020-10-30 安庆师范大学 T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof
CN114073241A (en) * 2021-11-11 2022-02-22 青岛农业大学 Observation device applied to parasitic wasps and manufacturing method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108174827A (en) * 2018-02-27 2018-06-19 福建农林大学 A kind of Liriomyza raising collection device and its application method
CN108174827B (en) * 2018-02-27 2022-11-08 福建农林大学 Breeding and collecting device for leaf miner and using method thereof
CN108293965A (en) * 2018-03-29 2018-07-20 福建省农业科学院植物保护研究所 A kind of method of field acquisition tea lesser leafhopper
CN111838077A (en) * 2019-04-28 2020-10-30 安庆师范大学 T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof
CN114073241A (en) * 2021-11-11 2022-02-22 青岛农业大学 Observation device applied to parasitic wasps and manufacturing method thereof
CN114073241B (en) * 2021-11-11 2023-02-07 青岛农业大学 Observation device applied to parasitic wasps and manufacturing method thereof

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