CN107711726A - A kind of egg parasitoidses raising collection device and collection method - Google Patents
A kind of egg parasitoidses raising collection device and collection method Download PDFInfo
- Publication number
- CN107711726A CN107711726A CN201711233953.2A CN201711233953A CN107711726A CN 107711726 A CN107711726 A CN 107711726A CN 201711233953 A CN201711233953 A CN 201711233953A CN 107711726 A CN107711726 A CN 107711726A
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- raising
- ovum
- fixed part
- tea
- collection device
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- 238000010010 raising Methods 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000011159 matrix material Substances 0.000 claims abstract description 18
- 241001122767 Theaceae Species 0.000 claims description 61
- 102000002322 Egg Proteins Human genes 0.000 claims description 50
- 108010000912 Egg Proteins Proteins 0.000 claims description 50
- 210000004681 ovum Anatomy 0.000 claims description 50
- 229920000742 Cotton Polymers 0.000 claims description 21
- 238000004804 winding Methods 0.000 claims description 17
- 241001414720 Cicadellidae Species 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 7
- 230000003071 parasitic effect Effects 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 241000819999 Nymphes Species 0.000 claims description 3
- -1 dult Species 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims 1
- 238000010276 construction Methods 0.000 abstract description 3
- 241000219146 Gossypium Species 0.000 description 15
- 241000317929 Chalcididae Species 0.000 description 8
- 241000256844 Apis mellifera Species 0.000 description 3
- 241000086608 Empoasca vitis Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 241000931705 Cicada Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000015459 Lycium barbarum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Food-Manufacturing Devices (AREA)
Abstract
The present invention relates to a kind of egg parasitoidses raising collection device and collection method, the device includes setting culture matrix, keeping portion upper end open in keeping portion, the fixed part positioned at middle part and the superposed raising portion of bottom, keeping portion;Fixing hole is set in the middle part of fixed part, and fixed part upper surface sets detent one, lower surface to set detent two;Raising portion is provided with provisions hole and passage, raises subordinate's end opening.This raising collection device is simple in construction, is easy to load and unload, cost is cheap, easy to operate, realizes the raising and collection of indoor egg parasitoidses.
Description
Technical field
This patent is related to a kind of egg parasitoidses raising collection device and collection method.
Background technology
Tea lesser leafhopper is one of most important insect of tea tree, and in the world, Main Tea leaf plantation country is distributed, every year
15-20% economic loss is brought to global Tea Production.Tealeaves is the health being becoming increasingly popular in a kind of world wide
Drink, as the tea lesser leafhopper of tea place primary pest, chemical pesticide is still depended in its preventing and treating, which unduly, causes tealeaves agriculture residual exceeded, directly
Connect the harm person's of drinking tea health, thus there is an urgent need to safely, effectively, sustainable replacement control prece.Tassel chalcid fly is that tea is small
The main egg parasitoidses of greenery cicada, two kinds of tassel chalcid flies are related generally to, be three rod tassel Nikols respectivelyStethyniumSp. and split
Bone tassel NikolSchizophragmaSp species.They are made ovum to hatch, subtracted by the ovum of parasitic tea lesser leafhopper
The quantity of tea lesser leafhopper is lacked, certain control action is played to tea lesser leafhopper population.Field investigation result shows that tassel is small
Honeybee tea place parasitic rate more than 50%, only it is noted that Sustainable use, certain natural contral is might have to tea lesser leafhopper and is made
With so as to reduce or even avoid the use of chemical pesticide during tea lesser leafhopper is prevented and treated, the safety of raising tea place production
Property.Therefore, it is both tassel chalcid flies of more preferable Sustainable use, the enquiry based work of tassel chalcid fly biology and ecological characteristics just shows
Obtain especially important.
Want the biology and ecological characteristics of investigation tassel chalcid fly, it is necessary first to obtain sufficient amount of tassel chalcid fly.
Tassel chalcid fly build is small, and body is about 0.5 mm, in tea place only be visually not easy observe.It is difficult by free-hand or conventional tool to want
To capture sufficient amount of tassel chalcid fly.Under study for action, adopt the tip more and take back interior, solution takes smaller green leaf hopper under the microscope in the past
Ovum, after put culture dish moisturizing culture wait the small bee eclosion of tassel go out ovum method collect needed for experiment material, technical difficulty is big, takes
When it is laborious.
The content of the invention
In view of the deficiencies of the prior art, the technical problems to be solved by the invention are to provide a kind of egg parasitoidses raising
Collection device and collection method, are not only simple in structure, and are easy to load and unload, and cost is cheap, and easy to operate, can realize indoors
A large amount of, efficient and convenient collection to egg parasitoidses.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme:A kind of egg parasitoidses raise collection device,
It includes setting culture matrix in portion positioned at the keeping portion of bottom, the fixed part positioned at middle part and superposed raising portion, keeping,
Keeping portion upper end open;Fixing hole is set in the middle part of fixed part, and fixed part upper surface sets detent one, lower surface to set positioning
Groove two;Raising portion is provided with provisions hole and passage, raises subordinate's end opening.
Further, passage is evenly and symmetrically arranged in the middle part of raising portion in the horizontal direction, and is sealed using gauze.
Further, provisions hole is arranged at the top of raising portion.
Further, the cotton with hydromel is set in provisions hole.
Further, keeping portion is tubbiness, and fixed part is tabular, and raising portion is spherical crown shape, and the circular section of spherical crown is down.
Further, keeping portion and raising portion use transparent plastics material.
Preferably, the height in the keeping portion is 8-15cm, and upper end open external diameter is 8-15cm;The a diameter of 3-4 of fixing hole
cm;The height in the raising portion is 9-16cm, and lower ending opening external diameter is 6-9cm, and provisions bore dia is 1cm, and passage is a diameter of
3-4cm, the aperture 80-100 mesh of gauze.
Further, the collection method of egg parasitoidses raising collection device, comprises the following steps:
Step S1:Fresh band ovum tea branch is taken, band ovum tea every tealeaves of branch is carried out to cut the processing of half leaf;
Step S2:Culture matrix is poured into keeping portion, the more plants of band ovum tea branches handled well through cutting half leaf are taken, by with ovum tea branch
Bottom limb is wound with winding cotton, and winding the position of cotton will be such that band ovum tea branch lower length is mutually fitted with keeping portion height
Answer, be highly adapted with ovum tea branch upper length and raising portion, the thickness for winding cotton also will be with the fixing hole in the middle part of fixed part
It is adapted, winding cotton position is determined and the fixing hole of fixed part is filled in after winding, and fixed part is placed in keeping portion, is protected
Card band ovum tea branch bottom is immersed to maintain band ovum tea branch growth in culture matrix, and keeping portion tip edge is inserted into determining for fixed part
In the groove two of position, fixed part is set to be fixed on keeping portion;
Step S3:Raising portion is placed on fixed part, makes the detent of the edge insertion fixed part of the lower openings in raising portion
In one, to ensure that raising portion is fixed on fixed part, the band leaf site with ovum tea branch is covered;
Step S4:Cotton with hydromel is filled in provisions hole to supplement food needed for egg parasitoidses;
Step S5:Culture matrix old in keeping portion is outwelled at regular intervals, changes fresh culture matrix to meet band ovum
Needed for tea branch growth, at the same time, raising portion is periodically gently opened, collects the egg parasitoidses sprouted wings in raising portion inwall;
Collection work continues until that band ovum tea branch is withered.
Further, the limb of 6-9cm or so under the spray and spray of slightly five leaves is remained with ovum tea branch;Take ten plants of bands
Ovum tea branch;Culture matrix was changed every three days;Raising portion is opened daily is collected work.
Further, while egg parasitoidses are collected, collected at the tealeaves back side hatched tea lesser leafhopper nymph and
Adult.
Compared with prior art, the present invention has following remarkable advantage:This raising collection device is simple in construction, is easy to fill
Unload, cost is cheap, easy to operate, can realize raising and collection to egg parasitoidses indoors;Keeping portion provides nutrient and water
Divide normal emergence naturally, the development for maintain needed for the survival of insert band ovum tea branch and growth, farthest ensureing egg parasitoidses
Needed for subsequent growth extra-nutrition;Raising portion provides space needed for the branches and leaves stretching, extension of band ovum and egg parasitoidses are sprouted wings and movable institute
Condition is needed, because parasitic wasp has the habit upwards and to photo-reactive, the parasitic wasp after generally sprouting wings is concentrated in raising spheroid
On wall, and it is transparent plastics material to raise ball material, the egg parasitoidses being thus easily observe that after sprouting wings, also causes parasitism
The collection work of honeybee is very convenient.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the embodiment of the present invention.
In figure:1- keepings portion, 2- fixed parts, 3- raisings portion, 4- culture matrixes, 5- provisions hole, cottons of the 6- with hydromel,
7- passages, 8- gauzes, 9- fixing holes, 10- detents one, 11- detents two, 12- band ovum tea branches, 13- winding cottons.
Embodiment
The present invention will be further described in detail with reference to the accompanying drawings and detailed description.
As shown in figure 1, the present invention provides a kind of egg parasitoidses raising collection device, it is characterised in that:The device is by supporting
Portion 1, fixed part 2 and the three parts of raising portion 3 composition.Keeping portion provides nutrient and moisture ensures the survival of insert band ovum tea branch and growth
It is required, raising portion provide space and egg parasitoidses needed for band ovum branches and leaves stretching, extension sprout wings and activity needed for condition, fixed position in
Between keeping portion and raising portion, for fixing and supporting tea branch egg parasitoidses.
The keeping portion is tubbiness, is surrounded by side wall and bottom, and upper end is open.Keeping portion inner bottom part is filled with culture
Matrix 4, for cultivating band ovum tea branch.The keeping portion uses high transparency plastic material, and the height in the keeping portion is 8-15cm,
Upper end open external diameter is 8-15cm.
The raising portion is spherical crown shape, and down, lower end is open for the circular section of raising portion spherical crown.The raising portion offers one
Individual provisions hole 5(The cm of Circularhole diameter 1), provisions hole 5 is stoppered using the cotton 6 with hydromel.In the middle part of the raising portion it is symmetrical and
4 passages 7 are uniformly arranged in the horizontal direction(Circularhole diameter 3-4 cm), with gauze 8(Aperture 80-100 mesh)Sealing, is used for
The ventilation in raising portion.The raising portion uses high transparency plastic material, and the height in the raising portion is 9-16cm, outside lower ending opening
Footpath is 6-9cm.
The fixed part is tabular, and middle part offers fixing hole 9, and upper surface offers the detent 1 of circle, under
Surface offers detent 2 11, and the diameter of the detent one is identical with raising portion lower openings external diameter, the positioning
The diameter of groove two is identical with keeping portion upper end open external diameter, the fixed part by being connected on detent one with raising portion,
It is connected by detent with keeping portion for two times, the fixing hole(Diameter 3-4 cm)For placing band ovum tea branch 12, the band
Ovum tea branch is fixed by winding cotton 13 and the fixing hole and its underpart is soaked in culture matrix.
The present invention also provides a kind of collection method of egg parasitoidses raising collection device, comprises the following steps:
Step S1:Fresh band ovum tea branch 12 is taken, it is left to ensure to remain with 6-9cm under the spray and spray of slightly five leaves with ovum tea branch 12
Right limb, band ovum tea every leaf of branch is carried out to cut the processing of half leaf;
Step S2:Culture matrix 4 is poured into keeping portion 2, takes 10 plants of band ovum tea branches handled well through cutting half leaf, will be with ovum tea branch
Limb is wound with winding cotton 13 below one slightly five leaves, and band ovum tea branch lower length will be made with supporting by winding the position of cotton
Portion is highly adapted, band ovum tea branch upper length and raising portion 3 are highly adapted, and the thickness for winding cotton also will be with fixed part 3
The fixing hole 9 at middle part is adapted, and winding cotton position is determined and the fixing hole of fixed part is filled in after winding, and fixed part is put
In in keeping portion, ensureing to immerse in culture matrix with maintenance with ovum tea branch growth with ovum tea branch bottom, by keeping portion tip edge
Insert in fixed part detent 2 11, make fixed part is seamless to be fixed on keeping portion;
Step S3:Raising portion is placed on fixed part, makes the detent of the edge insertion fixed part of the lower openings in raising portion
In one 10, it is fixed on ensureing that raising portion is seamless on fixed part, covers the position with slightly five leaves of ovum tea branch one;
Step S4:Cotton with hydromel is filled in provisions hole 5 to supplement food needed for parasitic wasp;
Step S5:Culture matrix old in keeping portion was outwelled every three days, changes fresh culture matrix to meet band ovum tea branch
Needed for growth, at the same time, raising portion is gently opened daily, parasitic wasp because of the characteristics of having light and up flight and usually can be
Movable habit on raising portion inwall, the egg parasitoidses sprouted wings can be collected in raising portion inwall, and according to smaller green leaf hopper
Like hiding and suck juice at the tealeaves back side, hatched tea lesser leafhopper nymph and adult can be collected at the tealeaves back side in passing.
Collection work can continue until that tea branch is withered, and usual tea branch can survive more than 20 days.
In summary, this raising collection device is simple in construction, is easy to load and unload, cost is cheap, easy to operate, can be in room
Raising and collection of the interior realization to egg parasitoidses.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (10)
1. a kind of egg parasitoidses raise collection device, it is characterised in that:Including positioned at the keeping portion of bottom, the fixation positioned at middle part
Portion and superposed raising portion, culture matrix, keeping portion upper end open are set in keeping portion;Fixing hole is set in the middle part of fixed part,
Fixed part upper surface sets detent one, lower surface to set detent two;Raising portion is provided with provisions hole and passage, raises
Support subordinate's end opening.
2. egg parasitoidses according to claim 1 raise collection device, it is characterised in that:Passage is uniform in the horizontal direction
And be symmetricly set in the middle part of raising portion, and sealed using gauze.
3. egg parasitoidses according to claim 1 or 2 raise collection device, it is characterised in that:Provisions hole is arranged on raising
At the top of portion.
4. egg parasitoidses according to claim 3 raise collection device, it is characterised in that:Band hydromel is set in provisions hole
Cotton.
5. the egg parasitoidses raising collection device according to claim 1 or 2 or 4, it is characterised in that:Keeping portion is tubbiness,
Fixed part is tabular, and raising portion is spherical crown shape, and the circular section of spherical crown is down.
6. the egg parasitoidses raising collection device according to claim 1 or 2 or 4, it is characterised in that:Keeping portion and raising portion
Use transparent plastics material.
7. the egg parasitoidses raising collection device according to claim 1 or 2 or 4, it is characterised in that:The height in the keeping portion
It is 8-15cm to spend for 8-15cm, upper end open external diameter;The a diameter of 3-4 cm of fixing hole;The height in the raising portion is 9-16cm,
Lower ending opening external diameter is 6-9cm, and provisions bore dia is 1cm, a diameter of 3-4cm of passage, the aperture 80-100 mesh of gauze.
8. the collection method of the egg parasitoidses raising collection device according to one of claim 1-7, it is characterised in that:Including
Following steps:
Step S1:Fresh band ovum tea branch is taken, band ovum tea every tealeaves of branch is carried out to cut the processing of half leaf;
Step S2:Culture matrix is poured into keeping portion, the more plants of band ovum tea branches handled well through cutting half leaf are taken, by with ovum tea branch
Bottom limb is wound with winding cotton, and winding the position of cotton will be such that band ovum tea branch lower length is mutually fitted with keeping portion height
Answer, be highly adapted with ovum tea branch upper length and raising portion, the thickness for winding cotton also will be with the fixing hole in the middle part of fixed part
It is adapted, winding cotton position is determined and the fixing hole of fixed part is filled in after winding, and fixed part is placed in keeping portion, is protected
Card band ovum tea branch bottom is immersed to maintain band ovum tea branch growth in culture matrix, and keeping portion tip edge is inserted into determining for fixed part
In the groove two of position, fixed part is set to be fixed on keeping portion;
Step S3:Raising portion is placed on fixed part, makes the detent of the edge insertion fixed part of the lower openings in raising portion
In one, to ensure that raising portion is fixed on fixed part, the band leaf site with ovum tea branch is covered;
Step S4:Cotton with hydromel is filled in provisions hole to supplement food needed for egg parasitoidses;
Step S5:Culture matrix old in keeping portion is outwelled at regular intervals, changes fresh culture matrix to meet band ovum
Needed for tea branch growth, at the same time, raising portion is periodically gently opened, collects the egg parasitoidses sprouted wings in raising portion inwall;
Collection work continues until that band ovum tea branch is withered.
9. the collection method of egg parasitoidses raising collection device according to claim 8, it is characterised in that:Band ovum tea branch is protected
Leave the limb of 6-9cm or so under the spray and spray of slightly five leaves;Take ten plants of band ovum tea branches;Culture medium was changed every three days
Matter;Raising portion is opened daily is collected work.
10. the collection method of egg parasitoidses raising collection device according to claim 9, it is characterised in that:Collecting ovum
While parasitic wasp, hatched tea lesser leafhopper nymph and adult are collected at the tealeaves back side.
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CN201711233953.2A CN107711726A (en) | 2017-11-30 | 2017-11-30 | A kind of egg parasitoidses raising collection device and collection method |
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CN201711233953.2A CN107711726A (en) | 2017-11-30 | 2017-11-30 | A kind of egg parasitoidses raising collection device and collection method |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108174827A (en) * | 2018-02-27 | 2018-06-19 | 福建农林大学 | A kind of Liriomyza raising collection device and its application method |
CN108293965A (en) * | 2018-03-29 | 2018-07-20 | 福建省农业科学院植物保护研究所 | A kind of method of field acquisition tea lesser leafhopper |
CN111838077A (en) * | 2019-04-28 | 2020-10-30 | 安庆师范大学 | T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof |
CN114073241A (en) * | 2021-11-11 | 2022-02-22 | 青岛农业大学 | Observation device applied to parasitic wasps and manufacturing method thereof |
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