CN108174827B - Breeding and collecting device for leaf miner and using method thereof - Google Patents
Breeding and collecting device for leaf miner and using method thereof Download PDFInfo
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- CN108174827B CN108174827B CN201810163432.2A CN201810163432A CN108174827B CN 108174827 B CN108174827 B CN 108174827B CN 201810163432 A CN201810163432 A CN 201810163432A CN 108174827 B CN108174827 B CN 108174827B
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- leaf miner
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- 241001517923 Douglasiidae Species 0.000 title claims abstract description 56
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 230000001488 breeding effect Effects 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 241000382353 Pupa Species 0.000 claims abstract description 13
- 241000238631 Hexapoda Species 0.000 claims abstract description 9
- 239000004677 Nylon Substances 0.000 claims abstract description 8
- 229920001778 nylon Polymers 0.000 claims abstract description 8
- 238000003976 plant breeding Methods 0.000 claims abstract description 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 239000000725 suspension Substances 0.000 claims abstract 4
- 235000012907 honey Nutrition 0.000 claims description 15
- 241000237942 Conidae Species 0.000 claims description 13
- 229920000742 Cotton Polymers 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 6
- 239000003337 fertilizer Substances 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 230000032669 eclosion Effects 0.000 claims description 3
- 230000035784 germination Effects 0.000 claims description 3
- 125000003003 spiro group Chemical group 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 description 17
- 241001273318 Phyllophila Species 0.000 description 4
- 241000255925 Diptera Species 0.000 description 3
- 241000238740 Dermatophagoides pteronyssinus Species 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012840 feeding operation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention relates to a leafminer feeding and collecting device and a using method thereof, and the device comprises a host plant breeding device for providing nutrients and water required by normal growth and breeding of leafminer host plants and fixing the roots of the host plants, wherein the upper end of the host plant breeding device is provided with a leafminer pupa collecting mechanism, the upper end surface of the leafminer pupa collecting mechanism is vertically provided with a support frame, the support frame is provided with suspension frames at intervals from top to bottom and in a staggered manner, the suspension frames are provided with feeding devices for feeding leafminer adults, and the device further comprises a nylon net insect feeding cage for covering the whole leafminer feeding and collecting device. The breeding and collecting device is simple in structure and convenient to operate, and can be used for realizing large-scale, efficient and convenient collection of the leaf miner indoors.
Description
Technical Field
The invention relates to a leaf miner feeding and collecting device and a using method thereof.
Background
The leaf miner belongs to Diptera order Dermatophagoides pteronyssinus, has wide host range, has generation overlapping phenomenon and serious harm. The larvae of the larvae have licking mouthparts, often submerge into leaf tissues, submerge into the leaf flesh tissues, form linear irregular submerged paths to influence the photosynthesis of the leaves, further cause the withered and yellow leaves, decompose chlorophyll, reduce sugar content and even fall off and die the seedlings, thereby causing economic loss. Third instar larvae often bore out of leaves into the soil and pupate. In order to research the leaf miner, a laboratory needs to establish a leaf miner population, but the problems of short service life of adults, difficulty in collecting pupae of the leaf miner, complex manual feeding operation and the like often occur in indoor feeding of the leaf miner at present. Therefore, how to scientifically and rapidly establish the indoor leaf miner population becomes a difficult problem to be solved urgently for each researcher.
Disclosure of Invention
The invention aims to provide a device for breeding and collecting leaf miners and a using method thereof.
The technical scheme of the invention is as follows: the utility model provides a leafminer raises collection device, breeds the device including the host plant who is used for providing leafminer host plant normal growth and breeds required nutrient, moisture and fixed host plant root, host plant breeds the upper end of device and is provided with leafminer pupa collection mechanism, the up end of leafminer pupa collection mechanism is vertical to be provided with the support frame, top-down interval and dislocation are provided with the mounted frame on the support frame, be provided with the raising device who is used for raising the leafminer adult on the mounted frame, still including being used for covering the nylon wire insect cage of raising outside whole leafminer raises collection device.
Further, host plant breeds device includes the round platform casing of invering, the lower extreme of round platform casing is sealed, and the upper end of round platform casing is provided with the hole that leaks, and the bottom of round platform casing is filled with the culture medium through high temperature sterilization.
Further, submerged leaf fly pupa collects mechanism is including setting up in host plant breeds the disk type body and the 4 fan-shaped trays of the truncated cone form of device upper end, the internal cross body with disk type body that is provided with of disk type, be provided with two "one" word sockets respectively along length direction on each limb board of ten fonts, correspond along the radius direction on the angular bisector of fan-shaped tray bottom and be provided with two and be used for with "one" word socket matched with "L" shape overhead kick.
Furthermore, the positive center of ten fonts is provided with annotates fertile mouthful, and the lateral part that lies in on the cross body annotates fertile mouthful is provided with the connecting pile that is used for with support frame lower extreme looks spiro union, fine sand has been laid in the fan-shaped tray.
Further, the feeding device comprises an inverted round platform-shaped box body, a strip-shaped opening is formed in the top of the inverted round platform-shaped box body, strip-shaped cotton stretching into the inverted round platform-shaped box body and provided with honey water is arranged in the strip-shaped opening, and the honey water is filled in the inverted round platform-shaped box body.
Furthermore, the length of the long-strip cotton inserted into the strip-shaped opening is 2/3 of the length of the long-strip cotton, and the honey water filled in the inverted round platform-shaped box body accounts for 10% of the volume of the inverted round platform-shaped box body.
A use method of the device applied to the feeding and collecting of the leaf miner comprises the following steps:
step S1: accelerating germination and raising seedling of host plant seeds of the leaf miner;
step S2: pouring a culture medium subjected to high-temperature sterilization into the circular truncated cone shell, taking 1-3 seedlings with the same growth vigor, allowing the roots of the seedlings to penetrate through the fertilizer injection port, and planting and supplying the seedlings into the circular truncated cone shell;
and step S3: after two true leaves of the host plant grow, putting 200 heads of the fly pupae into a fan-shaped tray, and standing for eclosion to form imagoes;
and step S4: adding honey water into the long-strip cotton and the inverted frustum-shaped box body, and transferring the whole leaf miner feeding and collecting device into a nylon insect cage;
step S5: the honey water in the inverted round platform-shaped box body is replaced every two days to meet the requirement of growth of the leaf miner;
step S6: starting from a road on which the leaf flies bite, the fan-shaped tray is checked every three days, fine sand in the fan-shaped tray is taken out, and the fly pupae are screened by a 100-mesh sample screen.
Compared with the prior art, the invention has the following advantages: the device for breeding and collecting the leaf miner is simple in structure, easy to assemble and disassemble, low in cost and convenient to operate, and can be used for collecting the leaf miner indoors in a large amount and efficiently and conveniently.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a top view of the fly pupa collecting mechanism of the present invention;
FIG. 3 is a side view of a fan tray of the present invention;
fig. 4 is a schematic structural view of the inverted circular truncated cone-shaped box body of the present invention.
Detailed Description
In order to make the aforementioned features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below, but the present invention is not limited thereto.
Refer to fig. 1 to 4
The utility model provides a leafminer raises collection device, breeds device 10 including being used for providing leafminer host plant normal growth and breeds required nutrient, moisture and the host plant of fixed host plant root, host plant breeds the upper end of device and is provided with leafminer pupa collection mechanism 20, the vertical support frame 30 that is provided with of up end that the mechanism was collected to leafminer pupa, top-down interval and dislocation set have mounted frame 31 on the support frame, be provided with the breeding device 40 that is used for raising the leafminer adult on the mounted frame, still including being used for the cover to establish the nylon wire insect cage outside whole leafminer raises collection device, play the effect of preventing other insect intrusions and adult escape outward through nylon wire insect cage.
In this embodiment, host plant breeds device includes the round platform casing of invering, the lower extreme of round platform casing is sealed, and the upper end of round platform casing is provided with the hole that leaks, and the bottom of round platform casing is filled with the culture medium through high temperature sterilization for cultivate leaf miner host plant. The circular truncated cone shell is made of high-strength plastic, the height of the circular truncated cone shell is 20 cm, and the outer diameter of an opening at the upper end of the circular truncated cone shell is 15 cm.
In this embodiment, submerged leaf fly pupa collection mechanism is including setting up 21 and 4 fan-shaped trays 22 of the disc-shaped body of the inverted round platform form that breeds the device upper end in host plant, the internal cross 23 that is provided with the disc-shaped body of disc-shaped, be provided with two "one" word sockets 24 respectively along length direction on each limb board of ten fonts, correspond along the radius direction on the angular bisector of fan-shaped tray bottom and be provided with two and be used for with "one" word socket matched with "L" shape overhead kick 25. The host plant breeding device and the leaf miner collecting mechanism are connected into a whole through the back-off structure.
In this embodiment, a fertilizer injection port 26 is disposed in the center of the cross body, and a connection pile 27 for screwing with the lower end of the support frame is disposed on the side of the cross body located on the fertilizer injection port, so as to mount the support frame. Fine sand is paved in the fan-shaped tray.
In this embodiment, the raising device includes an inverted circular truncated cone-shaped box body 41 (radius 3cm, height 3 cm), the top of the inverted circular truncated cone-shaped box body is provided with a strip-shaped opening 42 (length 1cm x width 0.5 cm), a strip-shaped cotton 43 which stretches into the inverted circular truncated cone-shaped box body and is provided with honey water is arranged in the strip-shaped opening, and the inverted circular truncated cone-shaped box body is further filled with the honey water so as to raise leaf miners.
In the embodiment, the length of the long-strip cotton inserted into the strip-shaped opening is 2/3 of the length of the long-strip cotton, and the honey water filled in the inverted circular truncated cone-shaped box body accounts for 10% of the volume of the inverted circular truncated cone-shaped box body.
A use method of the device applied to the feeding and collecting of the leaf miner comprises the following steps:
step S1: accelerating germination and raising seedling of host plant seeds of the leaf miner;
step S2: pouring a culture medium subjected to high-temperature sterilization into the circular truncated cone shell, taking 1-3 seedlings with the same growth vigor, allowing the roots of the seedlings to penetrate through the fertilizer injection port, and planting and cultivating in the circular truncated cone shell; rotating the cross body clockwise until the cross body is screwed, seamlessly fixing the four fan-shaped disc bodies on the cross body, and finally fixing the long rod-shaped support frame on the connecting pile of the cross body through a screw structure;
and step S3: after two true leaves of the host plant grow, putting 200 heads of the fly pupae into a fan-shaped tray, and standing for eclosion to form imagoes;
and step S4: the adult phyllophila dives breeding device is installed on the adult phyllophila dives pupa collecting mechanism, after the adult phyllophila dives breeding device is firmly fixed, honey water accounting for about 10% of the volume of the inverted truncated cone-shaped box body is added into the long-strip cotton and the inverted truncated cone-shaped box body, and then the whole adult phyllophila dives breeding and collecting device is transferred into a nylon insect breeding cage;
step S5: the honey water in the inverted round platform-shaped box body is replaced every two days to meet the requirement of growth of the leaf miner;
step S6: starting from a road on which the leaf flies bite, the fan-shaped tray is checked every three days, fine sand in the fan-shaped tray is taken out, the fly pupae are screened by a 100-mesh sieve, and the collection operation can be continued until the host dies.
The above description is only a preferred embodiment of the present invention, and it will be apparent to those skilled in the art that various forms of the device for feeding and collecting the leaf miner and the method for using the same do not require creative work based on the teaching of the present invention, and all the equivalent changes, modifications, substitutions and variations made in the claimed scope of the present invention should be covered by the present invention without departing from the principle and spirit of the present invention.
Claims (3)
1. A method for using a device for feeding and collecting the leaf miner comprises a host plant breeding device for providing nutrients and water required by normal growth and breeding of the leaf miner host plant and fixing the root of the host plant, a leaf miner pupa collecting mechanism is arranged at the upper end of the host plant breeding device, a support frame is vertically arranged on the upper end face of the leaf miner pupa collecting mechanism, suspension frames are arranged on the support frame in a staggered mode from top to bottom at intervals, a feeding device for feeding the adult leaf miner is arranged on the suspension frames, a nylon net insect feeding cage for covering the whole leaf miner feeding and collecting device is further included, the leaf miner pupa collecting mechanism comprises an inverted circular truncated cone-shaped disc-shaped body and 4 fan-shaped trays which are arranged at the upper end of the host plant breeding device, the internal cross body with the disk type body that is provided with of disk type, be provided with two "one" word sockets on each limb board of ten typefaces respectively along length direction, correspond along the radius direction on the angular bisector of fan-shaped tray bottom and be provided with two and be used for "L" shape overhead kick with "one" word socket matched with, the positive center of ten typefaces is provided with annotates fertile mouthful, and the lateral part that lies in on the cross body annotates fertile mouthful is provided with the connecting pile that is used for with support frame lower extreme looks spiro union, fine sand has been laid in the fan-shaped tray, raising device is including the round platform form box body that falls, the top of round platform form box body is provided with the strip opening, be provided with in the strip opening and stretch into the cotton that has honey water in the round platform form box body that falls, still fill honey water in the round platform form box body that falls, its characterized in that, include following step:
step S1: accelerating germination and raising seedling of host plant seeds of the leaf miner;
step S2: pouring a culture medium subjected to high-temperature sterilization into the circular truncated cone shell, taking 1-3 seedlings with the same growth vigor, allowing the roots of the seedlings to penetrate through the fertilizer injection port, and planting and supplying the seedlings into the circular truncated cone shell;
and step S3: after two true leaves of the host plant grow, putting 200 heads of the fly pupae into a fan-shaped tray, and standing for eclosion to form imagoes;
and step S4: adding honey water into the long-strip cotton and the inverted frustum-shaped box body, and transferring the whole leaf miner feeding and collecting device into a nylon insect cage;
step S5: the honey water in the inverted round platform-shaped box body is replaced every two days to meet the requirement of growth of the leaf miner;
step S6: starting from a road on which the leaf surface is gnawed by the leaf miner, checking the fan-shaped tray every three days, taking out fine sand in the fan-shaped tray, and screening the leaf miner pupae by using a 100-mesh sample screen.
2. The use method of the device for feeding and collecting the leaf miner as claimed in claim 1, wherein the host plant breeding device comprises an inverted circular truncated cone shell, the lower end of the circular truncated cone shell is closed, the upper end of the circular truncated cone shell is provided with a water leakage hole, and the bottom of the circular truncated cone shell is filled with a culture medium which is sterilized at high temperature.
3. The use method of the device for feeding and collecting the leaf miner as claimed in claim 1, wherein the length of the long cotton strips inserted into the strip-shaped openings is 2/3 of the length of the long cotton strips, and the honey water filled in the inverted circular truncated cone-shaped box body accounts for 10% of the volume of the inverted circular truncated cone-shaped box body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810163432.2A CN108174827B (en) | 2018-02-27 | 2018-02-27 | Breeding and collecting device for leaf miner and using method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810163432.2A CN108174827B (en) | 2018-02-27 | 2018-02-27 | Breeding and collecting device for leaf miner and using method thereof |
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| Publication Number | Publication Date |
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| CN108174827A CN108174827A (en) | 2018-06-19 |
| CN108174827B true CN108174827B (en) | 2022-11-08 |
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| JPH07255323A (en) * | 1994-03-18 | 1995-10-09 | Kubota Corp | Method for breeding natural enemy insect |
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Inventor after: Niu Dongsheng Inventor after: You Shijun Inventor after: Han Liwei Inventor after: Bai Jianlin Inventor after: Zhang Jie Inventor after: Li Jinyu Inventor after: You Minsheng Inventor before: Niu Dongsheng Inventor before: Han Liwei Inventor before: You Minsheng Inventor before: Bai Jianlin Inventor before: Zhang Jie Inventor before: Li Jinyu |