CN207544113U - A kind of use for laboratory drosophila culture and drug delivery device - Google Patents
A kind of use for laboratory drosophila culture and drug delivery device Download PDFInfo
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- CN207544113U CN207544113U CN201721671977.1U CN201721671977U CN207544113U CN 207544113 U CN207544113 U CN 207544113U CN 201721671977 U CN201721671977 U CN 201721671977U CN 207544113 U CN207544113 U CN 207544113U
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- epimere
- mouth
- hypomere
- threadedly coupled
- drosophila
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Abstract
The utility model is related to a kind of culture of use for laboratory drosophila and drug delivery devices, belong to use for laboratory drosophila experimental provision technical field, solve drosophila culture in the prior art and need replacing the technical problems such as different culture bottles from administration process, solution is：It is connected through a screw thread mouth respectively between epimere and stage casing, between stage casing and hypomere；Epimere upper end surface is equipped with bottle stopper, and liquid adding tube is connected through bottle stopper with epimere, and the middle part of epimere side wall is equipped with anesthetic air inlet one；The bottom of hypomere is equipped with culture medium, and the middle part of hypomere side wall is equipped with anesthesia gas air inlet two；It is threadedly coupled at mouth one and is equipped with sieve plate, be threadedly coupled at mouth two and be equipped with baffle, handle drives 9 o-clock positions to 12 o-clock positions of baffle or sieve plate in pointer circular rotating track to rotate by shaft.Present arrangement avoids do drosophila administration experimentation in need multiple links and different culture bottles the problem of, provide the integrated experimental provision of too many levels.
Description
Technical field
The utility model belongs to use for laboratory drosophila experimental provision technical field, more particularly to a kind of use for laboratory drosophila training
Foster and drug delivery device.
Background technology
Drosophila is classical mode biology, have the short period, easy raising, at low cost, breeding is fast, individual is small, Hou Daiduo, dyeing
The advantages such as body is few, character is apparent, genetic homology, be widely used in science of heredity and auxology correlative study, Parkinson's disease and Ah
The research and development of the nervous system disease agents such as Alzheimer's disease, drug habit, aging and service life, sleep and biological rhythm, learning and memory
Etc. research fields.
Research treatment disease medicament screening experiment in, through frequently with drosophila model organism as experimental subjects.Generally
It is needed according to experiment and drug feature is using pharmaceutical culture medium or the method for pipette tips administration.Adopt sometimes in experimentation by
Method of the drug mixing in culture medium carries out pharmaceutical intervention and treatment, and experimental implementation is more complicated, and being required for every time will be suitable
The drug of ratio is dissolved into culture medium.Using pipette tips medication, the step of making pharmaceutical culture medium, but this method are avoided
The step of being related to is relatively more, needs by multiple links such as feeding-empty stomach-administration-feedings, and each link is required for fruit
Fly is poured into different culture bottles, such as：Drosophila from the culture bottle equipped with culture medium is poured into empty culture bottle, it is small to stop 2
When drosophila is allowed to be in starvation；Drosophila is poured into another culture bottle again, drug injection is entered in pipette tips, pipette tips plug is existed
In culture bottle tampon, drosophila is fed；It also needs to pour into drosophila in the culture bottle equipped with culture medium again later.
In short, at present the common pipette tips medication in laboratory the shortcomings that be：
1st, it is frangible using glass culture bottle, often occur caused by culture bottle is crushed experimenter during the experiment
Injury, while also increase the cost of experiment.
2nd, in administration process, multiple links, each link such as feeding-empty stomach-administration-feeding is needed to be required for drosophila
Different culture bottles is poured into, needs to spend a large amount of time and efforts of experimenter.
3rd, the different culture bottles needed with a collection of drosophila need to carry out identical label, cause very big workload, and
The errors such as the culture bottle for drosophila being poured into mistake are susceptible to, influence the accuracy of experimental result.
4th, large batch of drosophila drug sieve is checked and verify and tested, there are many quantity for needing culture bottle, and it is empty to occupy very big experiment
Between, influence the progress of other experiments.
5th, drug sieve, which is checked and verify to test, needs a large amount of drosophila culture bottle, during cleaning culture bottle etc., largely occupies treasured
Expensive experimental period and manpower.
6th, it during administration, needs to pour into drosophila in different culture bottles, often results in drosophila and fly out experimental provision, shadow
Ring the quantity of experiment drosophila and the accuracy of experiment.
7th, existing administration pipette tips do not have reference mark, are unfavorable for recording the amount of drosophila intake drug, influence experimental result
Analysis.
Utility model content
The purpose of the utility model is to overcome the defects of the prior art, solve drosophila in the prior art to cultivate and tested
Journey needs replacing the technical issues of corresponding laboratory apparatus, provides a kind of use for laboratory drosophila culture and drug delivery device.
The utility model is achieved by the following technical programs.
A kind of use for laboratory drosophila culture and drug delivery device, it includes culture bottle with being threadedly coupled mouth, the threaded connection
Mouth includes being threadedly coupled mouth one with being threadedly coupled mouth two, wherein：The culture bottle includes epimere, stage casing and hypomere, and epimere is under
The upper surface of section is disposed as opening with lower face, and the upper surface of hypomere is set as open, and the lower face of hypomere is set as closed
End, is connected through a screw thread mouth one and connects, mouth two is connected through a screw thread between stage casing and hypomere and is connected between epimere and stage casing；
The upper end surface of the epimere is equipped with bottle stopper, and liquid adding tube is connected through bottle stopper with epimere, liquid adding tube
Side wall be provided with scale, the sharp nozzle end end of liquid adding tube is plugged with cotton-wool, be provided in the middle part of epimere side wall anesthetic into
Gas port one；
The bottom of the hypomere can add culture medium, and anesthesia gas air inlet two is provided in the middle part of hypomere side wall；
The side wall for being threadedly coupled mouth is provided with through-hole, and through-hole is provided with shaft, the external shaft end setting of shaft
There is handle, pointer is fixedly installed on the outer end of shaft, pointer circular rotating rail is located on the lateral wall of mouth is threadedly coupled
The o-clock position of mark is provided with limited block, and the o'clock position of pointer circular rotating track is located on the lateral wall in epimere and stage casing
The place of putting also is provided with limited block, and handle drives baffle in the o-clock position of pointer circular rotating track to o'clock by shaft
It rotates at position；
It is being threadedly coupled at mouth one：The other end of shaft is provided with sieve plate, and the lateral surface of sieve plate is with being threadedly coupled mouth one
Inner wall fit closely；
It is being threadedly coupled at mouth two：The other end of shaft is provided with baffle, and the outer rim of baffle is equipped with sealing ring, sealing ring
Lateral surface fitted closely in the inner wall for being threadedly coupled mouth two.
Further, the material of the sealing ring is silica gel.
Further, the anesthetic air inlet one and the anesthetic being passed through in anesthesia gas air inlet two are carbon dioxide gas
Body.
Further, the material of the epimere, stage casing and hypomere is transparent material, the material for the bottle stopper installed in epimere
For sponge or it is plastics.
Further, the material that the sieve plate is is rigid plastics, and mesh is provided on sieve plate, and mesh aperture is 1.5mm.
The utility model has the advantages that compared with prior art.
1st, transparent organic glass material may be used in culture bottle, non-friable, avoids in experimentation because culture bottle crushes
The injury caused by experimenter, while saved experimental cost.
2nd, reduce the number that drosophila is poured into different culture bottles, saved time cost for experimenter, reduced experiment
Flow.
3rd, large batch of drosophila drug sieve is checked and verify and tested, can largely reduced the quantity of required culture bottle, keep away
Exempt from excessive lab space waste, while reduce the culture bottle quantity for needing to clean, save manpower and resource.
4th, it can ensure that drosophila is present in a device in experimentation, avoid repeatedly falling experiment error caused by drosophila.
5th, the device can effectively avoid during administration drosophila from flying out experimental provision, ensure the quantity of drosophila and the standard of experiment
True property.
6th, the device facilitates observation dosage and residual drug, conducive to the amount of record drosophila intake drug, more accurately divides
Analyse drug dose and effect.
7th, the device can give drosophila different drugs simultaneously, convenient for preference or detest of the observation drosophila to drug, profit
It is adjusted in the experimental design in later stage and drug.
8th, carbon dioxide narcosis agent entrance is designed with, convenient for anaesthetizing drosophila after the administration is complete, all drosophilas are poured into it
He carries out subsequent experiment by experimental provision.Drosophila is avoided to fly out device, the drosophila quantity needed for experiment is not achieved, influences experiment knot
Fruit.
In short, the present apparatus provides a kind of drosophila culture and drug delivery device, solve in current drosophila administration experimentation
There are the problem of.
Description of the drawings
Fig. 1 is the overall structure diagram of drosophila cultivation stage in hypomere.
Fig. 2 is the overall structure diagram that drosophila is transferred to stage casing by hypomere.
The right side that Fig. 3 is Fig. 2 regards schematic cross-sectional view.
Fig. 4 is the overall structure diagram that drosophila stands the stage on an empty stomach in stage casing.
Fig. 5 is the overall structure diagram of drosophila dosed portions.
The right side that Fig. 6 is Fig. 5 regards schematic cross-sectional view.
Fig. 7 is partial enlargement structural representation when part A pointer coordinates with limited block in Fig. 6.
In figure, 1 is liquid adding tube, and 2 be bottle stopper, and 3 be epimere, and 4 be anesthetic air inlet one, and 5 be sieve plate, and 6 be screw thread
Interface one, 7 be stage casing, and 8 be baffle, and 9 be sealing ring, and 10 be hickey two, and 11 be hypomere, and 12 be anesthesia gas air inlet two,
13 be through-hole, and 14 be handle, and 15 be shaft, and 16 be pointer, and 17 be limited block, and 18 be culture medium.
Specific embodiment
It elaborates with reference to embodiment to the utility model：The present embodiment be using technical solutions of the utility model as
Premise is implemented, and gives detailed embodiment and specific operating process, but the scope of protection of the utility model is unlimited
In the following examples.
As shown in Figures 1 to 5, a kind of use for laboratory drosophila culture in the present embodiment and drug delivery device, it includes culture
Bottle includes being threadedly coupled mouth 1 with being threadedly coupled mouth 2 10 with being threadedly coupled mouth, the threaded connection mouth, wherein：The culture
Bottle includes epimere 3, stage casing 7 and hypomere 11, and the upper surface and lower face of epimere 3 and hypomere 11 are disposed as opening, hypomere 11
Upper surface is set as open, and the lower face of hypomere 11 is set as sealed end, mouth 1 is connected through a screw thread between epimere 3 and stage casing 7
Connection is connected through a screw thread the connection of mouth 2 10 between stage casing 7 and hypomere 11；
The upper end surface of the epimere 3 is equipped with bottle stopper 2, and liquid adding tube 1 is connected through bottle stopper 2 with epimere 3, and liquid adds
The side wall of pipe 1 is added to be provided with scale, from the upper end of liquid adding tube 1 by the sharp nozzle end end of cotton stopper to liquid adding tube 1,
Anesthetic air inlet 1 is provided in the middle part of 3 side wall of epimere；
The bottom of the hypomere 11 can add in culture medium 18, and anesthesia gas air inlet two is provided in the middle part of 11 side wall of hypomere
12；
The side wall for being threadedly coupled mouth is provided with through-hole 13, and through-hole 13 is provided with shaft 15, the outside of shaft 15
Shaft end is provided with handle 14, and pointer 16 is fixedly installed on the outer end of shaft 15, is located on the lateral wall of mouth is threadedly coupled
9 o-clock positions of 16 circular rotating track of pointer are provided with limited block 17, and epimere 3 refers to being located on the lateral wall in stage casing 7
12 o-clock positions of 16 circular rotating track of needle are also provided with limited block 17, and handle 14 drives baffle 8 to exist by shaft 15
9 o-clock positions to 12 o-clock positions of 16 circular rotating track of pointer rotate；
It is being threadedly coupled at mouth 1：The other end of shaft 15 is provided with sieve plate 5, and the lateral surface of sieve plate 5 is with being threadedly coupled
The inner wall of mouth 1 fits closely；
It is being threadedly coupled at mouth 2 10：The other end of shaft 15 is provided with baffle 8, and the outer rim of baffle 8 is equipped with sealing ring
9, the lateral surface of sealing ring 9 is fitted closely in the inner wall for being threadedly coupled mouth 2 10.
Further, the material of the sealing ring 9 is silica gel.
Further, the anesthetic being passed through in the anesthetic air inlet 1 and anesthetic air inlet 2 12 is titanium dioxide
Carbon gas.
Further, the material of the epimere 3, stage casing 7 and hypomere 11 is transparent material, the bottle stopper 2 installed in epimere 3
Material be sponge or be plastics.
Further, the material that the sieve plate 5 is is rigid plastics, and mesh is provided on sieve plate 5, and mesh aperture is
1.5mm。
The use process of the utility model is as follows：
First, it as shown in Figure 1, being connected through a screw thread mouth 2 10 is threadedly coupled on the upper surface for being installed on hypomere 11, opens
Baffle 8 is opened, Drosophila medium is put into hypomere 11, and drosophila is put into hypomere 11, closed baffle plate 8, by the lower end in stage casing 7
Face is connected through a screw thread the top for being installed on and being threadedly coupled mouth 2 10, and epimere 3 is installed on stage casing 7 by hickey 1
Bottle stopper 2 is filled in 3 upper surface open-mouth of epimere, closes sieve plate 5, mounted culture bottle is placed in drosophila by the top of upper surface
It is cultivated in suitable growing environment, treats that subsequent experimental uses；
Secondly, as shown in Figures 2 and 3, after drosophila feeding is abundant, blown from anesthesia gas air inlet 2 12 into hypomere 11
CO2Anesthetic gases.After drosophila is anaesthetized, culture bottle is inverted, opens baffle 8, drosophila is closed by being poured into stage casing 7 in hypomere 11
Baffle 8；
Again, as shown in figure 4, rotating and culturing bottle again, makes culture bottle just put, drosophila fully stands on an empty stomach in stage casing 7,
Meanwhile be inserted into liquid adding tube 1 in epimere 3 through bottle stopper 2, it walks and uses after remaining；
Finally, as shown in Figures 5 to 7, carbon dioxide narcosis gas is passed through into anesthetic air inlet 1 and anesthesia gas air inlet
Mouth 2 12 is blown into culture bottle together, opens sieve plate 5, and experimental drug is added dropwise into epimere 3 by the way that scale is quantitative with liquid adding tube 1
Product are observed；By each component disassembly, cleaning after experiment, next use is remained.
The above, only specific embodiment of the present utility model, but the scope of protection of the utility model is not limited to
In this, in any technical scope be familiar with those skilled in the art and disclosed in the utility model, the variation that can readily occur in
Or replace, it should be covered within the scope of the utility model.Therefore, the scope of protection of the utility model should be with the power
Subject to the protection domain of profit requirement.
Claims (5)
1. a kind of use for laboratory drosophila culture and drug delivery device, it includes culture bottle with being threadedly coupled mouth, the threaded connection mouth
Including being threadedly coupled mouth one（6）With being threadedly coupled mouth two（10）, it is characterised in that：The culture bottle includes epimere（3）, stage casing
（7）And hypomere（11）, epimere（3）And stage casing（7）Upper surface and lower face be disposed as opening, hypomere（11）Upper surface set
It is set to opening, hypomere（11）Lower face be set as sealed end, epimere（3）With stage casing（7）Between be connected through a screw thread mouth one（6）
Connection, stage casing（7）With hypomere（11）Between be connected through a screw thread mouth two（10）Connection；
The epimere（3）Upper end surface bottle stopper is installed（2）, liquid adding tube（1）Through bottle stopper（2）With epimere（3）Connection,
Liquid adding tube（1）Side wall be provided with scale, liquid adding tube（1）Sharp nozzle end end be plugged with cotton-wool, epimere（3）Side wall
Middle part is provided with anesthetic air inlet one（4）；
The hypomere（11）Bottom be pasted with culture medium（18）, hypomere（11）Anesthesia gas air inlet two is provided in the middle part of side wall
（12）；
The side wall for being threadedly coupled mouth is provided with through-hole（13）, through through-hole（13）It is provided with shaft（15）, shaft（15）'s
External shaft end is provided with handle（14）, shaft（15）Outer end on be fixedly installed pointer（16）, it is being threadedly coupled the outer of mouth
It is located at pointer on side wall（16）9 o-clock positions of circular rotating track are provided with limited block（17）, epimere（3）With stage casing
（7）Lateral wall on be located at pointer（16）12 o-clock positions of circular rotating track are also provided with limited block（17）, handle
（14）Pass through shaft（15）Drive baffle（8）In pointer（16）9 o-clock positions of circular rotating track are to 12 o-clock positions
It rotates；
It is being threadedly coupled mouth one（6）Place：Shaft（15）The other end be provided with sieve plate（5）, sieve plate（5）Lateral surface in screw thread
Connector one（6）Inner wall fit closely；
It is being threadedly coupled mouth two（10）Place：Shaft（15）The other end be provided with baffle（8）, baffle（8）Outer rim be equipped with it is close
Seal（9）, sealing ring（9）Lateral surface with being threadedly coupled mouth two（10）Inner wall fit closely.
2. a kind of use for laboratory drosophila culture according to claim 1 and drug delivery device, it is characterised in that：The sealing ring
（9）Material be silica gel.
3. a kind of use for laboratory drosophila culture according to claim 1 and drug delivery device, it is characterised in that：The anesthetic
Air inlet one（4）With anaesthetizing gas air inlet two（12）In the anesthetic that is passed through be carbon dioxide gas.
4. a kind of use for laboratory drosophila culture according to claim 1 and drug delivery device, it is characterised in that：The epimere
（3）, stage casing（7）And hypomere（11）Material be transparent material, epimere（3）The bottle stopper of middle installation（2）Material for sponge or
Person is plastics.
5. a kind of use for laboratory drosophila culture according to claim 1 and drug delivery device, it is characterised in that：The sieve plate
（5）Material for rigid plastics, sieve plate（5）On be provided with mesh, mesh aperture is 1.5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721671977.1U CN207544113U (en) | 2017-12-05 | 2017-12-05 | A kind of use for laboratory drosophila culture and drug delivery device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721671977.1U CN207544113U (en) | 2017-12-05 | 2017-12-05 | A kind of use for laboratory drosophila culture and drug delivery device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207544113U true CN207544113U (en) | 2018-06-29 |
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ID=62664824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721671977.1U Expired - Fee Related CN207544113U (en) | 2017-12-05 | 2017-12-05 | A kind of use for laboratory drosophila culture and drug delivery device |
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|---|---|
| CN (1) | CN207544113U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107864932A (en) * | 2017-12-05 | 2018-04-03 | 山西大医院 | Use for laboratory drosophila cultivates and doser |
-
2017
- 2017-12-05 CN CN201721671977.1U patent/CN207544113U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107864932A (en) * | 2017-12-05 | 2018-04-03 | 山西大医院 | Use for laboratory drosophila cultivates and doser |
| CN107864932B (en) * | 2017-12-05 | 2023-09-05 | 山西白求恩医院(山西医学科学院、华中科技大学同济医学院附属同济医院山西医院、山西医科大学第三医院、山西医科大学第三临床医学院) | Drosophila culture and drug administration device for laboratory |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180629 Termination date: 20191205 |