CN207699603U - A kind of suspension cell synchronizes the device of inoculation and dosing - Google Patents
A kind of suspension cell synchronizes the device of inoculation and dosing Download PDFInfo
- Publication number
- CN207699603U CN207699603U CN201721483636.1U CN201721483636U CN207699603U CN 207699603 U CN207699603 U CN 207699603U CN 201721483636 U CN201721483636 U CN 201721483636U CN 207699603 U CN207699603 U CN 207699603U
- Authority
- CN
- China
- Prior art keywords
- dosing
- sample
- suspension cell
- inoculation
- culture bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses the devices that a kind of suspension cell synchronizes inoculation and dosing, belong to biotechnology comprising culture bottle, the culture bottle have magnetic stirring apparatus, a magneton are housed in culture bottle；Two independent sample injectors, the sample injector are managed by a sample-adding and are connected in culture bottle respectively；Culture bottle inner wall is coated with silicone hydroxyl layer；Thermal isolation film is equipped between culture bottle and magnetic stirring apparatus.Magnetic stirring apparatus is arranged in the utility model, the suspension cell contributed in triangle bottles with flat bases is kept in motion always avoids suspension cell from reducing quantity because of hypoxia death, in the independent sample-adding pipeline of culture bottle cap setting, it can ensure not interfereing with each other suspension cell inoculation and dosing, whole process can manually control the quantity of inoculating cell or the volume of dosage, have splendid practicability.
Description
Technical field
The utility model is related to biotechnologies, and in particular to a kind of suspension cell synchronizes the device of inoculation and dosing.
Background technology
It is known that cell is the basic structure and functional unit of higher organism vital movement, the development of any individual
Journey is gradually assembled into different types of tissue, organ, system by countless functions and the cell to come in every shape, this complicated base
This process needs genetic control, while being also required to the positive induction of three-dimensional environment.In body three-dimensional environment
In, many growth factors, cell factor, adhesion factor, trophic factors etc. both participate in body cell and are gradually evolved into different type
Tissue, organ, system basic process in, it is seen then that the living environment of cell is very important for cell.
Research points out that cell culture refers to taking out cell, the appropriate microenvironment in analogue body, in nothing from body in-vivo tissue
Bacterium, acid-base value, provides under the primary condition of gas and certain nutrition nontoxic, proper temperature, enables cell growth, breeding, passes
In generation, freezes, and maintains a kind of culture technique of eucaryotic cell structure and function.In general, cell culture is modern biotechnology science example
Develop a kind of very rapid experimental technique, the technology in such as physiology, toxicology, pharmacology, cytology, histology science
Individual cells can gradually be cloned into cell mass, convenient for carrying out corresponding experimental study (Song Hong, Han Yunzhu, Cai Guo to cell
Progress [J] Zunyi Medical College journal of emblem cell culture technologies, 2014,37 (5)：549-554.), but cell culture
Working link is more and it is easy to appear contamination phenomenons.
Typically, existing cell culture includes mainly suspension cell culture, attached cell culture, wherein suspension cell
Culture needs cell to be in suspended state and needs sterile, nontoxic Essential Environment, when inoculation this requires suspension cell
Being strict with experiment operator emphasis aseptic concept, (sweet dew, Zhang Zhi, Lv Meina wait cell culture technologies [J] China group
Knit engineering research and clinical rehabilitation, 2008,12 (29)：5739-5742.), at the same in order to after suspension cell culture dosing it is smooth
It carries out, is also strict with experiment operator and focuses on aseptic concept, suspension cell inoculation, dosing are proposed as a result, higher
Basic experiment requirement.
During existing suspension cell culture, the phenomenon that often will appear pollution, in order to effectively improve suspend it is thin
Born of the same parents culture efficiency, quality, explore it is novel, efficient, avoid pollution cell culture relevant apparatus be just particularly important.
For some unpredictable difficulties of existing the relevant technologies factor is not can determine with some, the utility model proposes
A kind of suspension cell synchronizes the device of inoculation and dosing, these designs contribute to under the conditions of suspension cell progress normal sterile
It cultivates in right amount and remains that suspension cell is in suspended state, help to record the specific of suspension cell inoculation and dosing at any time
Time and the suspension cell culture time can be recorded, help to be directed to suspension cell inoculation and dosing both bases without interfering with each other
This experimental implementation and inoculating cell quantity and dosage are accurately calculated, helps to prevent other microbial contamination suspension cells and can
It is final to ensure that suspension cell inoculation and dosing are smoothed out active and effectively to maintain suspension cell to be in sterile microenvironment.
Invention content
To solve above-mentioned the deficiencies in the prior art, the utility model provides a kind of suspension cell and synchronizes inoculation and dosing
Device.
The technical solution of the utility model is as follows：
A kind of suspension cell synchronizes the device of inoculation and dosing, including
Culture bottle, the culture bottle have magnetic stirring apparatus, a magneton are housed in culture bottle；
Two independent sample injectors, the sample injector are managed by a sample-adding and are connected in culture bottle respectively；
The culture bottle inner wall is coated with silicone hydroxyl layer；
Thermal isolation film is equipped between the culture bottle and magnetic stirring apparatus.
Preferably, the culture bottle is triangle bottles with flat bases.
Preferably, the culture bottle includes bottle and bottle cap, and one layer of hydrophobic permeable membrane is equipped between the bottle and bottle cap.
Preferably, the thickness of the hydrophobic permeable membrane is 0.22 μm.
Preferably, one end that the sample-adding pipe is connect with culture bottle is sample-adding syringe needle.
Preferably, the bottle cap is equipped with two latex circular holes, is passed through for sample-adding syringe needle.
Preferably, the sample injector is piston injector, including sample injector outlet, sample-adding cylinder and piston；
The piston includes the round plug, intermediate circular pin and the oval handle of end of front end, circle plug and circular pin
In sample-adding cylinder.
Preferably, two sample injector is respectively used to inoculating cell and dosing.
Preferably, the sample-adding pipe is sealed with sample injector outlet connection by sealing buckle.
Preferably, the magnetic stirring apparatus side is equipped with time measurement device, and the face of the time measurement device is equipped with the time
Show screen, hour setting button, minute setting button, second setting button, time start button, time stop button, time
Be zeroed button and selection mode button.
Preferably, the culture bottle and sample injector outer surface are designed with scale value.
Preferably, the volume of the culture bottle is 150mL.
The utility model tool has the advantage that：
(1) volume of the triangle bottles with flat bases of the utility model is 150mL, and triangle bottles with flat bases inner surface has silicon hydroxyl
Base contributes to the free energy for reducing triangle bottles with flat bases inner surface, the suspension cell in triangle bottles with flat bases is avoided to go out
Existing sticking phenomenon, can effectively increase the quantity of suspension cell in the medium, avoid suspension cell dead because of anoxic
Phenomenon.
(2) there is triangle bottles with flat bases scale value in triangle bottles with flat bases outer surface, helps to observe the flat culture of triangle
The volume of suspension cell culture liquid in bottle.
(3) triangle bottles with flat bases lower inside has magnetic force to spoil son, and lower section is outside the bottom of bottle of triangle bottles with flat bases
Thermal isolation film, thermal isolation film lower section is small magnetic blender, and the suspension cell that these designs contribute in triangle bottles with flat bases begins
It is kept in motion eventually and small magnetic blender is avoided to generate thermal energy interference suspension cell culture, avoid suspension cell because lacking
Oxygen is dead and reduces quantity.
(4) the side standing time meter of small magnetic blender, the surface having time display screen of time measurement device,
Hour setting button, minute setting button, second setting button, time start button, time stop button, time zero button,
Selection mode button, these designs help to record the specific time of suspension cell inoculation and dosing at any time and can record suspension
The cell culture time.
(5) above triangle bottles with flat bases it is triangle bottles with flat bases bottle cap, triangle bottles with flat bases bottle and triangle are flat
There are hydrophobic permeable membrane and two latex circular holes, two latex circular holes to have inoculating cell syringe needle respectively, add between bottom culture bottle cap
Medicine syringe needle passes through, and inoculating cell syringe needle rear is inoculating cell needle handle, and Medicated needle of syringe rear is dosing needle handle, inoculating cell needle handle
With the conduit that is followed by of dosing needle handle, conduit connection sample injector exports and the two junction has sealing buckle to be sealed, and designs
Hydrophobic permeable membrane is 0.22 μm, it will help the culture microenvironment on the outside of triangle bottles with flat bases with suspension cell is isolated and may be used also
Think that in due course, appropriate, the suitable ventilation of suspension cell, two latex circular holes of design can ensure to suspension cell inoculation and dosing
It does not interfere with each other, and triangle bottles with flat bases can be sealed by the elastic switch of latex circular hole, suspension cell is avoided to pollute.
(6) it is sample injector outlet before sample injector, there is sample injector scale value on sample injector surface, has sample injector inside sample injector
Piston, sample injector piston are followed by sample injector and promote round bar, sample injector that round bar is promoted to be followed by sample injector ellipse handle, these set
In respect of help to be inoculated with suspension cell, dosing separately carries out and whole process can manually control inoculating cell quantity or
The volume of person's dosage.
Description of the drawings
Fig. 1 is the overall structure diagram of the utility model.
Fig. 2 is the Bottle cap structure schematic diagram of the taper culture bottle of the utility model.
Fig. 3 is the time measurement device operation panel schematic diagram of the utility model.
Reference sign：0- thermal isolation films；1- triangle bottles with flat bases；2- silicone hydroxyls subtract ergosphere；3- bottles with flat bases is carved
Angle value；4- inoculating cell syringe needles；5- Medicated needle of syringe；6- triangle bottles with flat bases bottle caps；7- hydrophobic permeable membranes；8- inoculating cell needles
Handle；9- dosing needle handles；10,21- conduits；11,22- sealings buckle；12,23- sample injectors export；13,24- sample injectors；14、25-
Sample injector piston；15,26- sample injectors scale value；16,27- sample injectors promote round bar；17,28- sample injectors ellipse handle；When 18-
Between meter；19- magnetic stirring apparatus；20- magnetic agitation rotors；29- hours setting buttons；30- minutes setting buttons；When 31-
Between show screen；32- seconds setting buttons；33- time start buttons；34- time stop buttons；The 35- times are zeroed button；36-
Selection mode button.
Specific implementation mode
In conjunction with implementation and the construction, shape and the further details of explanation of feature of Fig. 1-3 pairs of the utility model.It should manage
Solution, the present embodiment is merely to illustrate the utility model, rather than limits the scope of protection of the utility model.
The device of inoculation and dosing is synchronized for the suspension cell of the utility model as shown in Figure 1 comprising 1 triangle is flat
1,2 conduit (10,21) of culture bottle, 2 sealing buckles (11,22), 2 sample injectors (13,24), 1 magnetic force spoil son 20,1
19,1, the small magnetic blender time measurement device 18 of a thermal isolation film 0,1.Wherein, the volume of triangle bottles with flat bases 1 is
150mL, 1 inner surface of triangle bottles with flat bases have silicone hydroxyl to subtract ergosphere 2, and 1 lower inside of triangle bottles with flat bases has magnetic force to spoil
Son 20, it is that triangle is flat that, which there are triangle bottles with flat bases scale value 3,1 top of triangle bottles with flat bases in 1 outer surface of triangle bottles with flat bases,
Bottle cap 6 is cultivated at bottom, and lower section is thermal isolation film 0 outside the bottom of bottle of triangle bottles with flat bases 1, and 0 lower section of thermal isolation film is that small magnetic stirs
Mix device 19, the side standing time meter 18 of small magnetic blender 19, the surface having time display screen of time measurement device 18
Curtain 31, hour setting button 29, minute setting button 30, second setting button 32, time start button 33, time stop button
34, time zero button 35, selection mode button 36, as shown in Figure 3.As shown in Fig. 2, 1 bottle of triangle bottles with flat bases and three
The hydrophobic permeable membrane 7 and two latex circular holes (37,38), two latexes for having 0.22 μ m-thick between angle bottles with flat bases bottle cap 6 are justified
Hole (37,38) has inoculating cell syringe needle 4, Medicated needle of syringe 5 to pass through respectively.Such as Fig. 1,4 rear of inoculating cell syringe needle is inoculating cell
Needle handle 8,5 rear of Medicated needle of syringe are dosing needle handles 9, be respectively behind inoculating cell needle handle 8 and dosing needle handle 9 conduit (10,
21), conduit (10,21) connection sample injector outlet (12,23) and the two junction have sealing buckle (11,22) to be sealed, and add
It is sample injector outlet (12,23) before sample device (13,24), there is sample injector scale value (15,26) on sample injector (13,24) surface, adds
Have sample injector piston (14,25) inside sample device (13,24), sample injector piston (14,25) be followed by sample injector promote round bar (16,
27), sample injector promotes round bar (16,27) to be followed by sample injector ellipse handle (17,28).
The use detailed process of the utility model is as follows：
In use, design latex circular hole (37,38) is elastic material and can be closed automatically the utility model, if
Meter hydrophobic permeable membrane 7, which suitably can rationally ventilate, design thermal isolation film 0 avoids heat transfer mutual not to suspension cell, design two
Sample injector (13,24) of interference etc., very convenient suspension cell is inoculated with, dosing, this process need not unscrew suspension
The triangle bottles with flat bases bottle cap of cell culture and chemicals dosing plant, can avoid suspension cell from polluting active and effectively.
In suspension cell seeded process, round bar 16, sample injector piston are promoted by sample injector ellipse handle 17, sample injector
Suspension cell is sent by 14 manual strength transmission along sample injector 13, sample injector outlet 12, conduit 10, inoculating cell syringe needle 4
In triangle bottles with flat bases 1, drives magnetic force to spoil son 20 by small magnetic blender 19 and carry out dynamic cultivation.
During suspension cell dosing, round bar 27, sample injector piston are promoted by sample injector ellipse handle 28, sample injector
Suspension cell is sent into triangle by 25 manual strength transmission along sample injector 24, sample injector outlet 23, conduit 21, Medicated needle of syringe 5
In bottles with flat bases 1, drives magnetic force to spoil son 20 by small magnetic blender 19 and carry out mixing medicaments.
The description of the utility model is merely illustrative above, and not restrictive, those skilled in the art should be managed
Solution can carry out it within the spirit and scope that claim limits many modifications, variation or equivalent modification, but they are all
It will fall into the scope of protection of the utility model.
Claims (10)
1. a kind of suspension cell synchronizes the device of inoculation and dosing, which is characterized in that including
Culture bottle, the culture bottle have magnetic stirring apparatus, a magneton are housed in culture bottle；
Two independent sample injectors, the sample injector are managed by a sample-adding and are connected in culture bottle respectively；
The culture bottle inner wall is coated with silicone hydroxyl layer；
Thermal isolation film is equipped between the culture bottle and magnetic stirring apparatus.
2. suspension cell as described in claim 1 synchronizes the device of inoculation and dosing, which is characterized in that the culture bottle is three
Angle bottles with flat bases.
3. suspension cell as described in claim 1 synchronizes the device of inoculation and dosing, which is characterized in that the culture bottle includes
Bottle and bottle cap are equipped with one layer of hydrophobic permeable membrane between the bottle and bottle cap.
4. suspension cell as claimed in claim 3 synchronizes the device of inoculation and dosing, which is characterized in that the hydrophobic permeable membrane
Thickness be 0.22 μm.
5. suspension cell as described in claim 1 synchronizes the device of inoculation and dosing, which is characterized in that the sample-adding Guan Yupei
The one end for supporting bottle connection is sample-adding syringe needle.
6. suspension cell as claimed in claim 3 synchronizes the device of inoculation and dosing, which is characterized in that the bottle cap is equipped with
Two latex circular holes are passed through for sample-adding syringe needle.
7. suspension cell as described in claim 1 synchronizes the device of inoculation and dosing, which is characterized in that the sample-adding Guan Yujia
Sample device outlet connection is by sealing buckle sealing.
8. suspension cell as described in claim 1 synchronizes the device of inoculation and dosing, which is characterized in that the sample injector
For piston injector, including sample injector outlet, sample-adding cylinder and piston；
The piston includes the round plug, intermediate circular pin and the oval handle of end of front end, and circle plug and circular pin are located at
It is loaded in cylinder.
9. suspension cell as described in claim 1 synchronizes the device of inoculation and dosing, which is characterized in that described two independent sample-addings
Device is respectively used to inoculating cell and dosing.
10. suspension cell as described in claim 1 synchronizes the device of inoculation and dosing, which is characterized in that the magnetic agitation
Device side is equipped with time measurement device, and the face of the time measurement device is equipped with time showing screen, button is arranged in hour, minute sets
Set button, second setting button, time start button, time stop button, time zero button and selection mode button.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483636.1U CN207699603U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell synchronizes the device of inoculation and dosing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483636.1U CN207699603U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell synchronizes the device of inoculation and dosing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207699603U true CN207699603U (en) | 2018-08-07 |
Family
ID=63022207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721483636.1U Expired - Fee Related CN207699603U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell synchronizes the device of inoculation and dosing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207699603U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522099A (en) * | 2019-09-17 | 2021-03-19 | 上海联顺生物科技有限公司 | Cell culture bottle |
| CN112592812A (en) * | 2020-12-15 | 2021-04-02 | 山东省农业科学院畜牧兽医研究所 | Inoculation device for muscle cell culture |
-
2017
- 2017-11-08 CN CN201721483636.1U patent/CN207699603U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522099A (en) * | 2019-09-17 | 2021-03-19 | 上海联顺生物科技有限公司 | Cell culture bottle |
| CN112592812A (en) * | 2020-12-15 | 2021-04-02 | 山东省农业科学院畜牧兽医研究所 | Inoculation device for muscle cell culture |
| CN112592812B (en) * | 2020-12-15 | 2022-08-02 | 山东省农业科学院畜牧兽医研究所 | Inoculation device for muscle cell culture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207699603U (en) | A kind of suspension cell synchronizes the device of inoculation and dosing | |
| CN108456659A (en) | The preparation method of 3D brain organoids | |
| CN105624037A (en) | Method for establishing in-vitro blood-brain barrier model based on micro-fluidic chip | |
| CN207760352U (en) | Media to cell culture modules, culture solution module, cell cultivation chip and cell culture cabinet | |
| CN103735864B (en) | A kind of clinical laboratory antibacterial | |
| CN103805505B (en) | Organizational project myocardial cell is beaten and is pressed proofing unit and method | |
| CN216626575U (en) | Biological agent is at soil improvement device | |
| CN109082379A (en) | It is a kind of for highly emulating the in vitro culture device of Nasopharyngeal neoplasms | |
| CN104498391A (en) | Escherichia coli and culture method of culture medium thereof | |
| CN205669030U (en) | Co-culture of cells capsule | |
| CN104726333B (en) | Human pancreas cancer White Rabbit head of pancreas Transplanted tumor model builds and uses oncocyte preparation facilities | |
| CN211546489U (en) | Oral cavity biological film external anaerobic model device | |
| CN105112294A (en) | Coaxial multi-incubator bioreactor | |
| CN204607999U (en) | A kind of microbial ferment device | |
| CN207418767U (en) | A kind of suspension cell culture device being synchronously administered | |
| CN102399744A (en) | Method for culturing liver stem cells | |
| CN207544113U (en) | A kind of use for laboratory drosophila culture and drug delivery device | |
| CN207130275U (en) | A kind of co-culture device | |
| CN201823083U (en) | Nasal feeding input device | |
| CN202730134U (en) | Novel biology reactor | |
| CN201459106U (en) | Air duct microenvironment simulator for forming of bacterial biofilm | |
| CN205576165U (en) | Modified cell culture bottle | |
| CN207418762U (en) | A kind of drug resistant attached cell culture apparatus of inducing cell | |
| CN209338520U (en) | A kind of medical test microorganism incubator | |
| CN203559059U (en) | Full-automatic three-dimensional cell culturing system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180807 Termination date: 20191108 |