CN103805505B - Organizational project myocardial cell is beaten and is pressed proofing unit and method - Google Patents
Organizational project myocardial cell is beaten and is pressed proofing unit and method Download PDFInfo
- Publication number
- CN103805505B CN103805505B CN201410033418.2A CN201410033418A CN103805505B CN 103805505 B CN103805505 B CN 103805505B CN 201410033418 A CN201410033418 A CN 201410033418A CN 103805505 B CN103805505 B CN 103805505B
- Authority
- CN
- China
- Prior art keywords
- pressure
- nutrient solution
- myocardial
- myocardial cell
- set screw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N7/00—Analysing materials by measuring the pressure or volume of a gas or vapour
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
Abstract
The present invention relates to a kind of organizational project myocardial cell to beat pressure proofing unit and method.This device to myocardial cells culture room by the injection of uncontaminated air and the size ejecting the pressure regulating culturing room, then can measure the size of its pressure of beating by the situation of beating of microscopic examination myocardial cell under different pressure.When measuring, increase pressure, when observing culturing room's cardiac myocyte and stopping suddenly beating, value shown by tensimeter is exactly systolic pressure, then reduce pressure, to an instant observed myocardial cell and beat again, the value of now tensimeter display is exactly diastolic pressure.Because device have employed high-precision metrical instrument, be provided with minute-pressure setting device, the data recorded are accurately reliably.
Description
Technical field
The invention belongs to biomedical engineering field, relate to a kind of organizational project myocardial cell beat pressure proofing unit.By the size of pressure in fine adjustment myocardial cells culture room, observe myocardial cell to beat accordingly situation, design and develop the device of a set of dynamic pressure measurement of fighting about organizational project myocardial cell, particularly related to the important mechanical performance index of myocardial cell---the measurement of systolic pressure and diastolic pressure.Mechanical property measurement for biomedical engineering field organizational project myocardial cell provides a kind of new method.
Background technology
Cell therapy to heart stalk and whole latter stage cardiac disorder treatment there are bright prospects, but current effect is clinically not bery desirable, its one of the main reasons is exactly that the mechanical property of the myocardial cell of vitro culture is not mated with host cell, therefore the device of the mechanical characteristic of myocardial cell is measured in research and development, significant to the application of cell therapy on clinical medicine.
The most important function of myocardial cell is exactly pulsation and regular contractile motion, therefore measuring the mechanical characteristic of myocardial cell most important is exactly measure its pressure of beating---systolic pressure and diastolic pressure, but still do not measure myocardial cell at present and to beat the measuring apparatus of pressure.
Summary of the invention
The object of this invention is to provide a kind of size based on accurately changing pressure in myocardial cells culture room, observing the state of beating of corresponding myocardial cell, build measure myocardial cell beat pressure device.This device to myocardial cells culture room by the injection of uncontaminated air and the size ejecting the pressure regulating culturing room, then can measure the size of its pressure of beating by the situation of beating of microscopic examination myocardial cell under different pressure.When measuring, increase pressure, when observing culturing room's cardiac myocyte and stopping suddenly beating, value shown by tensimeter is exactly systolic pressure, then reduce pressure, to an instant observed myocardial cell and beat again, the value of now tensimeter display is exactly diastolic pressure.Because device have employed high-precision metrical instrument, be provided with minute-pressure setting device, the data recorded are accurately reliably.
The present invention includes organizational project myocardial cells culture room, measuring unit and minute-pressure regulon three parts.
Organizational project myocardial cells culture room comprises myocardial cells culture room, cultivates observation room cover plate, nutrient solution import, nutrient solution outlet, sealing plug and uncontaminated air entrance.
The top of myocardial cells culture room is transparent cultivation observation room cover plate, and the two ends of myocardial cells culture room are respectively nutrient solution import and nutrient solution outlet, and nutrient solution outlet is provided with sealing plug, and nutrient solution import is double does uncontaminated air entrance.
Measuring unit is made up of the first flexible pipe, instrument firm banking, manometer.
First soft pipe connection nutrient solution import, manometer is arranged on the pipeline of the first flexible pipe by instrument firm banking.
Minute-pressure regulon is made up of the second flexible pipe, pressure applying air bag, purging valve, the first set screw, the 4th set screw, fixation clip, pressure piston cylinder, cylinder supports frame, support baseboard, pressure piston, the second set screw, fixed support plate, horizontal micrometer, fixed support, horizontal fine motion slide unit, the 3rd set screw.
Second flexible pipe is by instrument firm banking and the first soft pipe connection; Pressure applies air bag and the second soft pipe connection, for applying pressure to culturing room; Purging valve is positioned at pressure and applies air bag is surveyed, the discharge of control pressure; Pressure piston cylinder is also hose connection with second; Pressure piston cylinder is fixed on cylinder supports frame by the first set screw and the 4th screw by fixation clip, and cylinder supports frame is connected with support baseboard again; Pressure piston and fixed support plate are fixed on one piece by the second set screw; Horizontal micrometer is connected with horizontal fine motion slide unit by fixed support, and horizontal fine motion slide unit is connected with fixed support plate by the 3rd set screw again.
Organizational project myocardial cell beat pressure measurement specifically comprise the following steps:
1, culturing room is taken off separately, put into seed cell, nutrient solution entrance, nutrient solution outlet are connected with nutrient solution pipeline, inject nutrient solution, under sterile environment, put into incubator cultured myocardial;
2, (observed the short cylinder cell of feature of beating by microscopes) after turning out myocardial cell, culturing room is connected with measurement below and micro-displacement unit.
3, close purging valve, press pressure applies air bag, pressurizes to culturing room.
4, by microscopic examination, observe myocardial cell beat faint time, stop press pressuring gasbag, use the horizontal micrometer of rotation instead, rotate forward at leisure, until myocardial cell beat stopping instant.The value now tensimeter shown is exactly systolic pressure.
5, open purging valve slowly to exit, observe myocardial cell slowly to recover to beat simultaneously, when beating more obvious, close purging valve, the horizontal micrometer of reverse rotation, rotation limit, limit is observed, until myocardial cell beats the most obviously and rotates small distance again when not changing, the value of now tensimeter display is diastolic pressure.
The present invention achieves the measurement of pressure of beating to myocardial cell first, simultaneously also by observing myocardial cell's number within the unit time, and then can obtain its frequency.Its advantage is simple to operate, and suitability is good, safe and reliable, and the mechanical property measurement for biomedical engineering field organizational project myocardial cell provides a kind of new method.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
As shown in Figure 1, the device of the pressure of beating of a kind of myocardial cell of measurement mainly contains culturing room's unit, and measuring unit, minute-pressure regulon 3 part form.
Culturing room's unit comprises sealing plug 1; Nutrient solution outlet 2; Uncontaminated air entrance (nutrient solution entrance) 22; Cultivate observation room cover plate 23; Myocardial cells culture room 24; 5th set screw 25.
Cultivate observation room cover plate 23 to be connected with myocardial cells culture room 24 by the 5th set screw 25.Have nutrient solution to export 2 at the left surface of myocardial cells culture room 24, there is sealing plug 1 in exit.Uncontaminated air entrance (nutrient solution entrance) 22 is had at myocardial cells culture room 24 right flank.The myocardial cell needed for us is cultivated in the effect of culturing room; It is transparent for cultivating observation room cover plate, so that our beating by microscopic examination myocardial cell; The effect of nutrient solution outlet to make nutrient solution flow out, and removes old nutrient solution; The effect of sealing plug be ensure when culturing room's pressure increases air tight; The effect of uncontaminated air entrance can make air inlet and outlet thus reach the object changing culturing room's pressure, and the input of nutrient solution is also complete on this mouth simultaneously, injects new nutrient solution.Whole myocardial cells culture reactor is sealing, avoids the impact of bacterium in contact outside air.
Measuring unit comprises the first flexible pipe 3; Instrument firm banking 4; Manometer composition 5.
First flexible pipe 3 left side connects uncontaminated air entrance (nutrient solution entrance) 22, and the right connects instrument firm banking 4; Manometer 5 is fixed on instrument firm banking 4.First soft pipe connection culturing room, ensure that the pressure of flexible pipe and the pressure of culturing room are consistent; The effect of instrument firm banking is fixation pressure instrument; Manometer can measure the pressure size on the flexible pipe at place accurately.
Minute-pressure regulon comprises the second flexible pipe 6, pressure applies air bag 21, purging valve 20, first set screw 7, the 4th set screw 18, fixation clip 8, pressure piston cylinder 9, cylinder supports frame 19, support baseboard 14, pressure piston 10, second set screw 11, fixed support plate 12, horizontal micrometer 13, fixed support 15, horizontal fine motion slide unit 16, the 3rd set screw 17 form.
Second flexible pipe 6 is communicated with the first flexible pipe 3 by instrument firm banking 4; Pressure applies air bag 21 and is communicated with the second flexible pipe 6; Purging valve 20 is positioned at pressure and applies air bag 21 is surveyed, the discharge of control pressure; Pressure piston cylinder 9 is connected with the second flexible pipe 6; Pressure piston cylinder 9 is fixed on cylinder supports frame 19 by the first set screw 7 and the 4th set screw 18 by fixation clip 8, and cylinder supports frame 19 is connected with support baseboard 14 again; Pressure piston 10 and fixed support plate 12 are fixed on one piece by the second set screw 11; Horizontal micrometer 13 is connected with horizontal fine motion slide unit 16 by fixed support 15, and horizontal fine motion slide unit 16 is connected with fixed support plate 12 by the 3rd set screw 17 again.Wherein the principle of horizontal micrometer and the principle of screw micrometer similar, by rotarily driving the movement of horizontal fine motion slide unit, thus promote piston, make cylinder produce micro-pressure.
Organizational project myocardial cell beat pressure measurement specifically comprise the following steps:
1, culturing room is taken off separately, put into seed cell, nutrient solution entrance, nutrient solution outlet are connected with nutrient solution pipeline, inject nutrient solution, under sterile environment, put into incubator cultured myocardial.
2, (observed the short cylinder cell of feature of beating by microscopes) after turning out myocardial cell, culturing room is connected with measurement below and micro-displacement unit.
3, close purging valve, press pressure applies air bag, pressurizes to culturing room.
4, by microscopic examination, observe myocardial cell beat faint time, stop press pressuring gasbag, use the horizontal micrometer of rotation instead, rotate forward at leisure, until myocardial cell beat stopping instant.The value now tensimeter shown is exactly systolic pressure.
5, open purging valve slowly to exit, observe myocardial cell slowly to recover to beat simultaneously, when beating more obvious, close purging valve, the horizontal micrometer of reverse rotation, rotation limit, limit is observed, until myocardial cell beats the most obviously and rotates small distance again when not changing, the value of now tensimeter display is diastolic pressure.
Claims (2)
1. organizational project myocardial cell is beaten pressure proofing unit, comprises organizational project myocardial cells culture room, measuring unit and minute-pressure regulon three parts, it is characterized in that:
Organizational project myocardial cells culture room comprises myocardial cells culture room, cultivates observation room cover plate, nutrient solution import, nutrient solution outlet, sealing plug and uncontaminated air entrance;
The top of myocardial cells culture room is transparent cultivation observation room cover plate, and the two ends of myocardial cells culture room are respectively nutrient solution import and nutrient solution outlet, and nutrient solution outlet is provided with sealing plug, and nutrient solution import is double does uncontaminated air entrance;
Measuring unit is made up of the first flexible pipe, instrument firm banking, manometer;
First soft pipe connection nutrient solution import, manometer is arranged on the pipeline of the first flexible pipe by instrument firm banking;
Minute-pressure regulon is made up of the second flexible pipe, pressure applying air bag, purging valve, the first set screw, the 4th set screw, fixation clip, pressure piston cylinder, cylinder supports frame, support baseboard, pressure piston, the second set screw, fixed support plate, horizontal micrometer, fixed support, horizontal fine motion slide unit, the 3rd set screw;
Second flexible pipe is by instrument firm banking and the first soft pipe connection; Pressure applies air bag and the second soft pipe connection, for applying pressure to culturing room; Purging valve is positioned at pressure and applies on the upside of air bag, the discharge of control pressure; Pressure piston cylinder is also hose connection with second; Pressure piston cylinder is fixed on cylinder supports frame by the first set screw and the 4th set screw by fixation clip, and cylinder supports frame is connected with support baseboard again; Pressure piston and fixed support plate are fixed on one piece by the second set screw; Horizontal micrometer is connected with horizontal fine motion slide unit by fixed support, and horizontal fine motion slide unit is connected with fixed support plate by the 3rd set screw again.
2. organizational project myocardial cell is beaten pressure detection method, adopts organizational project myocardial cell as claimed in claim 1 to beat pressure proofing unit, it is characterized in that the method is specifically:
1, organizational project myocardial cells culture room is taken off separately, put into seed cell, nutrient solution import, nutrient solution outlet are connected with nutrient solution pipeline, inject nutrient solution, under sterile environment, put into incubator cultured myocardial;
2, after turning out myocardial cell, culturing room is connected with measurement below and minute-pressure regulon;
3, close purging valve, press pressure applies air bag, pressurizes to organizational project myocardial cells culture;
4, by microscopic examination, observe myocardial cell beat faint time, stop press pressuring gasbag, use the horizontal micrometer of rotation instead, rotate forward at leisure, until myocardial cell beat stopping instant; The value now tensimeter shown is exactly systolic pressure;
5, open purging valve slowly to exit, observe myocardial cell slowly to recover to beat simultaneously, when beating more obvious, close purging valve, the horizontal micrometer of reverse rotation, rotation limit, limit is observed, until myocardial cell beats the most obviously and rotates small distance again when not changing, the value of now tensimeter display is diastolic pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410033418.2A CN103805505B (en) | 2014-01-24 | 2014-01-24 | Organizational project myocardial cell is beaten and is pressed proofing unit and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410033418.2A CN103805505B (en) | 2014-01-24 | 2014-01-24 | Organizational project myocardial cell is beaten and is pressed proofing unit and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103805505A CN103805505A (en) | 2014-05-21 |
| CN103805505B true CN103805505B (en) | 2015-07-29 |
Family
ID=50702897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410033418.2A Active CN103805505B (en) | 2014-01-24 | 2014-01-24 | Organizational project myocardial cell is beaten and is pressed proofing unit and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103805505B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263804B (en) * | 2014-09-15 | 2016-06-08 | 浙江大学 | A kind of myocardial cell mechanical beats detection method of optically-based graphical analysis |
| CN104388311B (en) * | 2014-11-20 | 2016-09-21 | 杭州电子科技大学 | Organizational project stem cell inducing cardiomyocytes is cultivated and measures bioreactor |
| CN116286315B (en) * | 2023-03-21 | 2023-10-27 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2606811Y (en) * | 2003-03-10 | 2004-03-17 | 刘佐庭 | Plunger type high precision micro injection pump |
| CN201305602Y (en) * | 2008-10-21 | 2009-09-09 | 中国人民解放军军事医学科学院卫生装备研究所 | Bionic-type myocardial tissue bioreactor |
| CN203768361U (en) * | 2014-01-24 | 2014-08-13 | 杭州电子科技大学 | Tissue engineering myocardial cell pulse pressure detecting device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7439057B2 (en) * | 2004-11-16 | 2008-10-21 | La Jolla Bioengineering Institute | Convective flow tissue assembly |
-
2014
- 2014-01-24 CN CN201410033418.2A patent/CN103805505B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2606811Y (en) * | 2003-03-10 | 2004-03-17 | 刘佐庭 | Plunger type high precision micro injection pump |
| CN201305602Y (en) * | 2008-10-21 | 2009-09-09 | 中国人民解放军军事医学科学院卫生装备研究所 | Bionic-type myocardial tissue bioreactor |
| CN203768361U (en) * | 2014-01-24 | 2014-08-13 | 杭州电子科技大学 | Tissue engineering myocardial cell pulse pressure detecting device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103805505A (en) | 2014-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103179875B (en) | Cosmetic bio-cellulose mask pack sheet and method for manufacturing same and preparation method thereof | |
| CN103805505B (en) | Organizational project myocardial cell is beaten and is pressed proofing unit and method | |
| WO2019019526A1 (en) | Microfluidic chip and application thereof | |
| JPS60500699A (en) | Analysis of cytotoxicity in cell culture equipment | |
| CN102399693B (en) | Simulation three-dimensional cell cultivation device and cultivation method | |
| CN103898058B (en) | A kind of three-dimensional culture method of novel gum knurl stem cell and its application | |
| CN102676446B (en) | Method and device for loading cell fluid stress on deformable curved surface and experimental platform | |
| CN201268704Y (en) | Dynamic bionic pulsating movement bioreactor | |
| CN203768361U (en) | Tissue engineering myocardial cell pulse pressure detecting device | |
| CN108373974B (en) | Chip device for three-dimensional cell culture and in-situ real-time myocardial tissue monitoring and application thereof | |
| CN101824382A (en) | Tissue engineering myocardium bioreactor constructed by pouring, perfusion and pulsation combination | |
| CN104480009B (en) | A kind of bioreactor device with perfusion and stretching dual-use function | |
| CN207502096U (en) | A set of mechanics parameter for engineering blood vessel culture monitors system | |
| CN104120083B (en) | A kind of organism culturing device | |
| CN108378873A (en) | A kind of Neurology clinic liquid taking device | |
| CN204311067U (en) | A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor | |
| CN101475908A (en) | Cultivation apparatus and method for implementing continuous tension stress action to cell | |
| CN204039417U (en) | A kind of organism culturing device | |
| CN104388311B (en) | Organizational project stem cell inducing cardiomyocytes is cultivated and measures bioreactor | |
| CN101120959B (en) | Application of guhong injection for treating lung cancer | |
| CN206609633U (en) | A kind of preclinical medicine check sampling device | |
| CN105950466A (en) | Endangium experimental apparatus capable of correcting stress relaxation of elastic base | |
| CN220625664U (en) | Disposable sterile injection needle air tightness detection device | |
| CN205741064U (en) | A kind of tunica intima elastic substrates culture systems | |
| CN202011878U (en) | Flow chamber system capable of realizing high pressure stream sterilization and regulating pressure and shearing force |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20161014 Address after: 411101 high tech building, Furong Middle Road, high tech Zone, Hunan, Xiangtan, China, No. 9 Patentee after: Xiangtan poly energy saving environmental technology Co., Ltd. Address before: Hangzhou City, Zhejiang province 310018 Xiasha Higher Education Park No. 2 street Patentee before: Hangzhou Electronic Science and Technology Univ |
|
| C56 | Change in the name or address of the patentee | ||
| CP03 | Change of name, title or address |
Address after: Aspen street, Hangzhou Economic Development Zone, Zhejiang province 310018 No. 6 Street No. 452 building 3 room 716 Patentee after: Hangzhou Bai Qiao medical skill company limited Address before: 411101 high tech building, Furong Middle Road, high tech Zone, Hunan, Xiangtan, China, No. 9 Patentee before: Xiangtan poly energy saving environmental technology Co., Ltd. |