CN103805505A - Device and method for detecting pulsating pressure of tissue engineering myocardial cell - Google Patents
Device and method for detecting pulsating pressure of tissue engineering myocardial cell Download PDFInfo
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- CN103805505A CN103805505A CN201410033418.2A CN201410033418A CN103805505A CN 103805505 A CN103805505 A CN 103805505A CN 201410033418 A CN201410033418 A CN 201410033418A CN 103805505 A CN103805505 A CN 103805505A
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N7/00—Analysing materials by measuring the pressure or volume of a gas or vapour
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Abstract
The invention relates to a device and a method for detecting the pulsating pressure of a tissue engineering myocardial cell. According to the device, the size of the pressure intensity of a myocardial cell culture room can be regulated by respectively injecting and discharging clean air in and out of the myocardial cell culture room, and the size of the pulsating pressure of the myocardial cell is measured by observing pulsating conditions of the myocardial cell by virtue of a microscope at the different pressure intensities; in the measurement process, the pressure intensity is increased, when an operator observes that the myocardial cell in the culture room stops beating suddenly, a value displayed by a pressure meter is a systolic pressure, then the pressure intensity is decreased until reaching a moment when the operator observes that the myocardial cell beats again, and the value displayed by the pressure meter at the moment is a diastolic pressure. As the device is provided with high-precision measuring instruments and a micro-pressure regulating device, the measured data is accurate and reliable.
Description
Technical field
The invention belongs to biomedical engineering field, relate to a kind of organizational project myocardial cell beat press proofing unit.By the size of pressure in fine adjustment myocardial cell culturing room, observe myocardial cell's situation of beating accordingly, design and develop a set ofly about the fight device of dynamic pressure measurement of organizational project myocardial cell, particularly related to myocardial cell's important mechanical performance index---the measurement of systolic pressure and diastolic pressure.For biomedical engineering field organizational project myocardial cell's mechanical property measurement provides a kind of new method.
Background technology
Cell therapy to heart stalk and whole latter stage cardiac disorder treatment there are bright prospects, but current effect is clinically not bery desirable, its one of the main reasons is exactly that the myocardial cell's of vitro culture mechanical property is not mated with host cell, therefore the device of myocardial cell's mechanical characteristic is measured in research and development, and the application to cell therapy on clinical medicine is significant.
The most important function of myocardial cell be exactly pulsation be regular contractile motion, therefore measuring myocardial cell's mechanical characteristic most important is exactly to measure its pressure of beating---systolic pressure and diastolic pressure, but does not still measure myocardial cell's measuring apparatus of pressing of beating at present.
Summary of the invention
The object of this invention is to provide a kind of size based on accurately changing pressure in myocardial cell culturing room, observe corresponding myocardial cell's the state of beating, build measure myocardial cell beat press device.This device can be to myocardial cell culturing room by the injection of uncontaminated air with eject the size of pressure that regulates culturing room, then the situation of beating under different pressure is measured its size of pressing of beating by microscopic examination myocardial cell.In the time measuring, increase pressure, in the time observing culturing room's cardiac myocyte cut and beat, the shown value of tensimeter is exactly systolic pressure, then reduce pressure, to an instant of observing myocardial cell and beating again, the value that now tensimeter shows is exactly diastolic pressure.Because device has adopted high-precision metrical instrument, be provided with minute-pressure setting device, the data that record are accurately reliably.
The present invention includes organizational project myocardial cell culturing room, measuring unit and three parts of minute-pressure regulon.
Organizational project myocardial cell culturing room comprises myocardial cell culturing room, cultivates observation room cover plate, nutrient solution import, nutrient solution outlet, sealing plug and uncontaminated air entrance.
The top of myocardial cell culturing room is transparent cultivation observation room cover plate, and the two ends of myocardial cell culturing room are respectively nutrient solution import and nutrient solution outlet, in nutrient solution outlet, are provided with sealing plug, the double uncontaminated air entrance that does of nutrient solution import.
Measuring unit is made up of the first flexible pipe, instrument firm banking, manometer.
The first soft pipe connection nutrient solution import, manometer is arranged on the pipeline of the first flexible pipe by instrument firm banking.
Minute-pressure regulon applies air bag, purging valve, the first set screw, the 4th set screw, fixation clip, pressure piston cylinder, cylinder supports frame, support baseboard, pressure piston, the second set screw, fixed support plate, horizontal micrometer, fixed support, horizontal fine motion slide unit, the 3rd set screw by the second flexible pipe, pressure and forms.
The second flexible pipe is by instrument firm banking and the first soft pipe connection; Pressure applies air bag and the second soft pipe connection, for culturing room is exerted pressure; Purging valve is positioned at pressure and applies on air bag and survey, the discharge of control pressure; Pressure piston cylinder is also hose connection with second; Fixation clip is fixed to pressure piston cylinder on cylinder supports frame by the first set screw and the 4th screw, and cylinder supports frame is connected with support baseboard again; Pressure piston and fixed support plate are fixed on one by the second set screw; Horizontal micrometer is connected with horizontal fine motion slide unit by fixed support, and horizontal fine motion slide unit is connected with fixed support plate by the 3rd set screw again.
Organizational project myocardial cell beat press measurement specifically comprise the following steps:
1, culturing room is taken off separately, put into seed cell, nutrient solution entrance, nutrient solution outlet are connected with nutrient solution pipeline, inject nutrient solution, under sterile environment, put into incubator and cultivate myocardial cell;
2, turn out after myocardial cell and (observe the short cylinder cell of the feature of beating by microscopes), culturing room is connected with measurement and micrometric displacement unit below.
3, close purging valve, press pressure applies air bag, pressurizes to culturing room.
4, by microscopic examination, observe beating of myocardial cell when faint, stop by pressuring gasbag, use the horizontal micrometer of rotation instead, be rotated in the forward at leisure, until myocardial cell's the instant of beating and stopping.The value now showing on tensimeter is exactly systolic pressure.
5, opening purging valve slowly exits, observe myocardial cell slowly recovers to beat simultaneously, when beating when more obvious, close purging valve, the horizontal micrometer of reverse rotation, rotation limit, limit is observed, until myocardial cell beats the most obviously and rotates small distance while not changing again, the value that now tensimeter shows is diastolic pressure.
The present invention has realized the measurement of pressing of beating to myocardial cell first, simultaneously also can be by observe myocardial cell's number within the unit time, and then obtain its frequency.Its advantage is simple to operate, and suitability is good, safe and reliable, for biomedical engineering field organizational project myocardial cell's mechanical property measurement provides a kind of new method.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
As shown in Figure 1, a kind of myocardial cell's of measurement the device of pressing of beating mainly contains culturing room unit, measuring unit, minute-pressure regulon 3 part compositions.
Culturing room unit comprises sealing plug 1; Nutrient solution outlet 2; Uncontaminated air entrance (nutrient solution entrance) 22; Cultivate observation room cover plate 23; Myocardial cell culturing room 24; The 5th set screw 25.
Cultivating observation room cover plate 23 is connected with myocardial cell culturing room 24 by the 5th set screw 25.Have nutrient solution outlet 2 at the left surface of myocardial cell culturing room 24, there is sealing plug 1 in exit.There is uncontaminated air entrance (nutrient solution entrance) 22 at myocardial cell culturing room 24 right flanks.The effect of culturing room is to cultivate our required myocardial cell; It is transparent cultivating observation room cover plate, so that our beating by microscopic examination myocardial cell; The effect of nutrient solution outlet is to make nutrient solution flow out, and removes old nutrient solution; The effect of sealing plug be in the time that culturing room pressure increases, guarantee air tight; Thereby the effect of uncontaminated air entrance is to make air inlet and outlet reach the object that changes culturing room's pressure, and the input of nutrient solution is simultaneously also to complete on this mouthful, injects new nutrient solution.Whole myocardial cell cultivates reactor and seals, and has avoided the impact of bacterium in contact outside air.
Measuring unit comprises the first flexible pipe 3; Instrument firm banking 4; Manometer composition 5.
First flexible pipe 3 left sides connect uncontaminated air entrance (nutrient solution entrance) 22, and the right connects instrument firm banking 4; Manometer 5 is fixed on instrument firm banking 4.The first soft pipe connection culturing room, has guaranteed that the pressure of flexible pipe and the pressure of culturing room are consistent; The effect of instrument firm banking is fixation pressure instrument; Manometer can be measured the pressure size on the flexible pipe at place accurately.
Minute-pressure regulon comprises that the second flexible pipe 6, pressure apply air bag 21, purging valve 20, the first set screw 7, the 4th set screw 18, fixation clip 8, pressure piston cylinder 9, cylinder supports frame 19, support baseboard 14, pressure piston 10, the second set screw 11, fixed support plate 12, horizontal micrometer 13, fixed support 15, horizontal fine motion slide unit 16, the 3rd set screw 17 and forms.
The second flexible pipe 6 is communicated with the first flexible pipe 3 by instrument firm banking 4; Pressure applies air bag 21 and is communicated with the second flexible pipe 6; Purging valve 20 is positioned at pressure and applies survey on air bag 21, the discharge of control pressure; Pressure piston cylinder 9 is connected with the second flexible pipe 6; Fixation clip 8 is fixed to pressure piston cylinder 9 on cylinder supports frame 19 by the first set screw 7 and the 4th set screw 18, and cylinder supports frame 19 is connected with support baseboard 14 again; Pressure piston 10 and fixed support plate 12 are fixed on one by the second set screw 11; Horizontal micrometer 13 is connected with horizontal fine motion slide unit 16 by fixed support 15, and horizontal fine motion slide unit 16 is connected with fixed support plate 12 by the 3rd set screw 17 again.Wherein the principle of horizontal micrometer and the principle of screw micrometer are similar, by rotarily driving the movement of horizontal fine motion slide unit, thereby promote piston, make cylinder produce micro-pressure.
Organizational project myocardial cell beat press measurement specifically comprise the following steps:
1, culturing room is taken off separately, put into seed cell, nutrient solution entrance, nutrient solution outlet are connected with nutrient solution pipeline, inject nutrient solution, under sterile environment, put into incubator and cultivate myocardial cell.
2, turn out after myocardial cell and (observe the short cylinder cell of the feature of beating by microscopes), culturing room is connected with measurement and micrometric displacement unit below.
3, close purging valve, press pressure applies air bag, pressurizes to culturing room.
4, by microscopic examination, observe beating of myocardial cell when faint, stop by pressuring gasbag, use the horizontal micrometer of rotation instead, be rotated in the forward at leisure, until myocardial cell's the instant of beating and stopping.The value now showing on tensimeter is exactly systolic pressure.
5, opening purging valve slowly exits, observe myocardial cell slowly recovers to beat simultaneously, when beating when more obvious, close purging valve, the horizontal micrometer of reverse rotation, rotation limit, limit is observed, until myocardial cell beats the most obviously and rotates small distance while not changing again, the value that now tensimeter shows is diastolic pressure.
Claims (2)
1. the organizational project myocardial cell pressure proofing unit of beating, comprises organizational project myocardial cell culturing room, measuring unit and three parts of minute-pressure regulon, it is characterized in that:
Organizational project myocardial cell culturing room comprises myocardial cell culturing room, cultivates observation room cover plate, nutrient solution import, nutrient solution outlet, sealing plug and uncontaminated air entrance;
The top of myocardial cell culturing room is transparent cultivation observation room cover plate, and the two ends of myocardial cell culturing room are respectively nutrient solution import and nutrient solution outlet, in nutrient solution outlet, are provided with sealing plug, the double uncontaminated air entrance that does of nutrient solution import;
Measuring unit is made up of the first flexible pipe, instrument firm banking, manometer;
The first soft pipe connection nutrient solution import, manometer is arranged on by instrument firm banking on the pipeline of the first flexible pipe;
Minute-pressure regulon applies air bag, purging valve, the first set screw, the 4th set screw, fixation clip, pressure piston cylinder, cylinder supports frame, support baseboard, pressure piston, the second set screw, fixed support plate, horizontal micrometer, fixed support, horizontal fine motion slide unit, the 3rd set screw by the second flexible pipe, pressure and forms;
The second flexible pipe is by instrument firm banking and the first soft pipe connection; Pressure applies air bag and the second soft pipe connection, for culturing room is exerted pressure; Purging valve is positioned at pressure and applies on air bag and survey, the discharge of control pressure; Pressure piston cylinder is also hose connection with second; Fixation clip is fixed to pressure piston cylinder on cylinder supports frame by the first set screw and the 4th screw, and cylinder supports frame is connected with support baseboard again; Pressure piston and fixed support plate are fixed on one by the second set screw; Horizontal micrometer is connected with horizontal fine motion slide unit by fixed support, and horizontal fine motion slide unit is connected with fixed support plate by the 3rd set screw again.
2. the organizational project myocardial cell pressure detection method of beating, adopts the organizational project myocardial cell as claimed in claim 1 pressure proofing unit of beating, and it is characterized in that the method is specifically:
1, organizational project myocardial cell culturing room is taken off separately, put into seed cell, nutrient solution import, nutrient solution outlet are connected with nutrient solution pipeline, inject nutrient solution, under sterile environment, put into incubator and cultivate myocardial cell;
2, turn out after myocardial cell, culturing room is connected with measurement and minute-pressure regulon below;
3, close purging valve, press pressure applies air bag, cultivates pressurization to organizational project myocardial cell;
4, by microscopic examination, observe beating of myocardial cell when faint, stop by pressuring gasbag, use the horizontal micrometer of rotation instead, be rotated in the forward at leisure, until myocardial cell's the instant of beating and stopping; The value now showing on tensimeter is exactly systolic pressure;
5, opening purging valve slowly exits, observe myocardial cell slowly recovers to beat simultaneously, when beating when more obvious, close purging valve, the horizontal micrometer of reverse rotation, rotation limit, limit is observed, until myocardial cell beats the most obviously and rotates small distance while not changing again, the value that now tensimeter shows is diastolic pressure.
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| CN103805505B CN103805505B (en) | 2015-07-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263804A (en) * | 2014-09-15 | 2015-01-07 | 浙江大学 | Myocardial cell mechanical beating detection method based on optical image analysis |
| CN104388311A (en) * | 2014-11-20 | 2015-03-04 | 杭州电子科技大学 | Bioreactor for culturing and measuring tissue engineering stem cell-induced myocardial cells |
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2606811Y (en) * | 2003-03-10 | 2004-03-17 | 刘佐庭 | Plunger type high precision micro injection pump |
| US20060105452A1 (en) * | 2004-11-16 | 2006-05-18 | The La Jolla Bioengineering Institute | Convective flow tissue assembly |
| CN201305602Y (en) * | 2008-10-21 | 2009-09-09 | 中国人民解放军军事医学科学院卫生装备研究所 | Bionic-type myocardial tissue bioreactor |
| CN203768361U (en) * | 2014-01-24 | 2014-08-13 | 杭州电子科技大学 | Tissue engineering myocardial cell pulse pressure detecting device |
-
2014
- 2014-01-24 CN CN201410033418.2A patent/CN103805505B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2606811Y (en) * | 2003-03-10 | 2004-03-17 | 刘佐庭 | Plunger type high precision micro injection pump |
| US20060105452A1 (en) * | 2004-11-16 | 2006-05-18 | The La Jolla Bioengineering Institute | Convective flow tissue assembly |
| CN201305602Y (en) * | 2008-10-21 | 2009-09-09 | 中国人民解放军军事医学科学院卫生装备研究所 | Bionic-type myocardial tissue bioreactor |
| CN203768361U (en) * | 2014-01-24 | 2014-08-13 | 杭州电子科技大学 | Tissue engineering myocardial cell pulse pressure detecting device |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263804A (en) * | 2014-09-15 | 2015-01-07 | 浙江大学 | Myocardial cell mechanical beating detection method based on optical image analysis |
| CN104263804B (en) * | 2014-09-15 | 2016-06-08 | 浙江大学 | A kind of myocardial cell mechanical beats detection method of optically-based graphical analysis |
| CN104388311A (en) * | 2014-11-20 | 2015-03-04 | 杭州电子科技大学 | Bioreactor for culturing and measuring tissue engineering stem cell-induced myocardial cells |
| CN104388311B (en) * | 2014-11-20 | 2016-09-21 | 杭州电子科技大学 | Organizational project stem cell inducing cardiomyocytes is cultivated and measures bioreactor |
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
| CN116286315B (en) * | 2023-03-21 | 2023-10-27 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
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| CN103805505B (en) | 2015-07-29 |
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Effective date of registration: 20161014 Address after: 411101 high tech building, Furong Middle Road, high tech Zone, Hunan, Xiangtan, China, No. 9 Patentee after: Xiangtan poly energy saving environmental technology Co., Ltd. Address before: Hangzhou City, Zhejiang province 310018 Xiasha Higher Education Park No. 2 street Patentee before: Hangzhou Electronic Science and Technology Univ |
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Address after: Aspen street, Hangzhou Economic Development Zone, Zhejiang province 310018 No. 6 Street No. 452 building 3 room 716 Patentee after: Hangzhou Bai Qiao medical skill company limited Address before: 411101 high tech building, Furong Middle Road, high tech Zone, Hunan, Xiangtan, China, No. 9 Patentee before: Xiangtan poly energy saving environmental technology Co., Ltd. |