CN205741064U - A kind of tunica intima elastic substrates culture systems - Google Patents
A kind of tunica intima elastic substrates culture systems Download PDFInfo
- Publication number
- CN205741064U CN205741064U CN201620437001.7U CN201620437001U CN205741064U CN 205741064 U CN205741064 U CN 205741064U CN 201620437001 U CN201620437001 U CN 201620437001U CN 205741064 U CN205741064 U CN 205741064U
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- China
- Prior art keywords
- elastic substrates
- tunica intima
- culture systems
- substrates culture
- upper plate
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Abstract
This utility model relates to a kind of tunica intima elastic substrates culture systems, this system includes elastic substrates culture chamber, analog and parameters measuring and controlling apparatus, described elastic substrates culture chamber is interconnected and form closed circuit with analog head and the tail, described parameters measuring and controlling apparatus connects elastic substrates culture chamber, described elastic substrates culture chamber includes upper plate assembly and lower shoe assembly, upper and lower bottom deck assembly is uniformly compressed by screw, forms the cell culturing space of a flowing.Compared with prior art, this utility model can monitor the data such as the temperature in culture systems, flow, pressure in real time, closer to human body environment.
Description
Technical field
This utility model relates to a kind of tunica intima elastic substrates culture systems, and especially a kind of elastic substrates is permissible
There is provided close to the tensile stress in human vas, the culture systems of tensile strain environment for tunica intima.
Background technology
The fatality rate of cardiovascular disease be higher than tumor, be the mankind the first killers, and the structure of tunica intima, function,
Gene expression etc. are in close relations with cardiovascular disease.Research shows, blood vessel lining cells can identify that what blood flow produced cuts
Stress, direct stress and tensile stress, and these mechanical signals are delivered to cell interior thus stimulate cell form,
The change of function and gene expression etc..Between variation and cardiovascular disease for further investigation blood vessel lining cells structure
Relation, need blood vessel lining cells is cultivated.Due to the complexity studied at body, set up a kind of close to people
The tunica intima experiment in vitro device of body blood mechanical environment is necessary.
Utility model content
The purpose of this utility model is to provide a kind of temperature stabilization, tunica intima that fidelity is high for the problems referred to above
Elastic substrates culture systems.
The purpose of this utility model can be achieved through the following technical solutions:
A kind of tunica intima elastic substrates culture systems, this system includes elastic substrates culture chamber, analog and ginseng
Number measure and control device, described elastic substrates culture chamber is interconnected and form closed circuit with analog head and the tail, and described parameter is surveyed
Control device connects elastic substrates culture chamber respectively.
Described elastic substrates culture chamber includes upper plate assembly and lower shoe assembly, and upper and lower bottom deck assembly passes through screw
Uniformly compress, form the cell culturing space of a flowing.
Described upper plate assembly includes upper plate and the inlet laying respectively at upper plate two ends and liquid outlet, described
Inlet, liquid outlet and analog are connected to form loop.
It is additionally provided with transparent windows on described upper plate.
Described lower shoe assembly includes lower shoe and the elastic substrates being located on lower shoe, pedestal, roller, shaft coupling
Device and motor, described elastic substrates one end is fixed on pedestal, and the other end is fixed on roller, described roller
It is connected with motor by shaft coupling.
Described elastic substrates is silica gel piece.
Described parameters measuring and controlling apparatus includes being positioned at the pulling force sensor of elastic substrates and the fixed connection place of pedestal, is positioned at
Photoelectric sensor at the wheel shaft of roller, the pressure transducer being positioned at upper plate two ends, the flow that is positioned at inlet pass
Sensor, the temperature sensor being positioned at liquid outlet and the computer being all connected with above-mentioned all the sensors.
Described analog includes heart pump and the accumulator being sequentially connected with.
Described monitoring device is computer.
Compared with prior art, this utility model has the advantages that
(1) this utility model uses temperature sensor to monitor in real time, can be that cell cultivates offer temperature phase
To stable pulsating flow surrounding；
(2) in this utility model driven by motor roller rotate realize elastic substrates stretch, can be cell provide connect
The tensile stress that is bordering in human vas, Zhang Bianli environment；
(3) this utility model uses pressure transducer simultaneous measuring apparatus inner proximal and the pressure of far-end, i.e. surveys
Obtaining simulation heart force value proximally and distally, fidelity is higher.
(4) this utility model use pulling force sensor and photoelectric sensor to measure tensile stress that elastic substrates is subject to and
Dependent variable, can find whether elastic substrates relaxes in time, and be modified in time.
Accompanying drawing explanation
Fig. 1 is system construction drawing of the present utility model；
Fig. 2 is the lower shoe schematic diagram of elastic substrates culture chamber；
Fig. 3 is the upper plate schematic diagram of elastic substrates culture chamber；
Wherein, 1 is elastic substrates, and 2 is roller, and 3 is shaft coupling, and 4 is motor, and 5 is pedestal, and 6 are
Inlet, 7 is liquid outlet, and 8 is transparent windows, and 9 is pulling force sensor, and 10 is photoelectric sensor, and 11 are
Pressure transducer, 12 is flow transducer, and 13 is temperature sensor, and 14 is heart pump, and 15 is accumulator.
Detailed description of the invention
With specific embodiment, this utility model is described in detail below in conjunction with the accompanying drawings.The present embodiment is new with this practicality
Implement premised on type technical scheme, give detailed embodiment and concrete operating process, but this practicality
Novel protection domain is not limited to following embodiment.
As it is shown in figure 1, the present embodiment provides a kind of tunica intima elastic substrates culture systems, train including elastic substrates
Support chamber, analog and parameters measuring and controlling apparatus.Analog includes accumulator 15 and the heart pump 14 being connected.
Elastic substrates culture chamber is uniformly compressed by screw by upper plate assembly and lower shoe assembly and forms.Such as Fig. 3
Shown in, upper plate assembly includes that upper plate, upper plate are provided with inlet 6, liquid outlet 7, transparent windows 8.
As in figure 2 it is shown, lower shoe assembly includes lower shoe, by pressing plate, elastic substrates 1 one end is fixed on pedestal 5
On, the other end is fixed on roller 2, and the wheel shaft of roller 2 is connected with motor 4 by shaft coupling 3.
The external accumulator 15 of liquid in-out mouth 6,7 and heart pump 14 etc. form loop, cultivate offer for tunica intima and connect
The steady pulsation stream environment of person of modern times's body vascular environment.Elastic substrates 1 is stretched indirectly for tunica intima by motor 4
Tensile stress, tensile strain are provided.Accumulator 15 scalable gas-liquid ratio, the simulation regulation to blood pressure；Heart pump 14
The periodicity Ejection function of simulation heart, produces pulsating flow, by the buffering of accumulator 15, intermittence is pulsed
Circulation is changed to seriality pulsating flow.
Parameters in parameters measuring and controlling apparatus real-time inspection and control culture chamber, the cultivation for tunica intima provides one
Close to human vas temperature, the environment of flow.Parameters measuring and controlling apparatus include pulling force sensor 9, photoelectric sensor 10,
Pressure transducer 11, flow transducer 12, temperature sensor 13 and computer, wherein, pulling force sensor 9
It is positioned at the fixed connection place of elastic substrates 1 and pedestal 5, for measuring the tensile stress that elastic substrates 1 is subject to；Photoelectricity
Sensor 10 is positioned at the wheel shaft of roller 2, for measuring the dependent variable of elastic substrates 1；Pressure transducer 11
Be provided with two, lay respectively at the inlet 6 of upper plate, liquid outlet 7, be used for measuring culture chamber inner proximal and
The pressure of far-end；Flow transducer 12 is positioned at inlet 6, for measuring the instantaneous delivery in culture chamber with total
Body flow；Temperature sensor 13 is positioned at liquid outlet 7, is used for measuring culture-liquid temp；Computer connects respectively
Pulling force sensor 9, photoelectric sensor 10, pressure transducer 11, flow transducer 12 and temperature sensor 13,
Receive the parameters in described culture chamber.
In elastic substrates culture chamber, the forward and reverse rotation of motor 4 drives roller 2 to rotate, and drives elastic substrates 1
Do stretching motion, thus provide tensile stress to the tunica intima cultivated in elastic substrates 1.Before stretching, system will
Elastic substrates 1 is stretched to an initial position, and now pulling force sensor 11 records value of thrust is 0N (elastic substrates
1 is in critical state).In drawing process, the tension of the elastic substrates 1 that pulling force sensor 11 is surveyed, by meter
Add and draw the strain value of elastic substrates 1.Stress-strain diagram can show on computers.
In incubation, the cultivation conditions of tunica intima can carry out real-time monitored by transparent windows 8.
Claims (8)
1. a tunica intima elastic substrates culture systems, it is characterised in that this system includes that elastic substrates is cultivated
Chamber, analog and parameters measuring and controlling apparatus, described elastic substrates culture chamber is interconnected and form circulation with analog head and the tail
Loop, described parameters measuring and controlling apparatus connects elastic substrates culture chamber.
Tunica intima elastic substrates culture systems the most according to claim 1, it is characterised in that described bullet
Property substrate culture chamber include upper plate assembly and lower shoe assembly, upper and lower bottom deck assembly is uniformly compressed by screw,
Form the cell culturing space of a flowing.
Tunica intima elastic substrates culture systems the most according to claim 2, it is characterised in that on described
Bottom deck assembly includes upper plate and the inlet laying respectively at upper plate two ends and liquid outlet, described inlet, goes out
Liquid mouth and analog are connected to form loop.
Tunica intima elastic substrates culture systems the most according to claim 3, it is characterised in that on described
Transparent windows it is additionally provided with on base plate.
Tunica intima elastic substrates culture systems the most according to claim 3, it is characterised in that under described
Bottom deck assembly includes lower shoe and the elastic substrates being located on lower shoe, pedestal, roller, shaft coupling and stepping electricity
Machine, described elastic substrates one end is fixed on pedestal, and the other end is fixed on roller, and described roller passes through shaft coupling
It is connected with motor.
Tunica intima elastic substrates culture systems the most according to claim 5, it is characterised in that described bullet
Property substrate is silica gel piece.
Tunica intima elastic substrates culture systems the most according to claim 5, it is characterised in that described ginseng
Number measure and control device includes being positioned at the pulling force sensor of the fixed connection place of elastic substrates and pedestal, being positioned at the wheel shaft of roller
The photoelectric sensor at place, it is positioned at the pressure transducer at upper plate two ends, is positioned at the flow transducer of inlet, is positioned at
The temperature sensor of liquid outlet and the computer being all connected with above-mentioned all the sensors.
Tunica intima elastic substrates culture systems the most according to claim 1, it is characterised in that described mould
Intend heart pump and accumulator that device includes being sequentially connected with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620437001.7U CN205741064U (en) | 2016-05-13 | 2016-05-13 | A kind of tunica intima elastic substrates culture systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620437001.7U CN205741064U (en) | 2016-05-13 | 2016-05-13 | A kind of tunica intima elastic substrates culture systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205741064U true CN205741064U (en) | 2016-11-30 |
Family
ID=57366805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620437001.7U Expired - Fee Related CN205741064U (en) | 2016-05-13 | 2016-05-13 | A kind of tunica intima elastic substrates culture systems |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205741064U (en) |
-
2016
- 2016-05-13 CN CN201620437001.7U patent/CN205741064U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161130 Termination date: 20190513 |