CN203768361U - Tissue engineering myocardial cell pulse pressure detecting device - Google Patents
Tissue engineering myocardial cell pulse pressure detecting device Download PDFInfo
- Publication number
- CN203768361U CN203768361U CN201420045380.6U CN201420045380U CN203768361U CN 203768361 U CN203768361 U CN 203768361U CN 201420045380 U CN201420045380 U CN 201420045380U CN 203768361 U CN203768361 U CN 203768361U
- Authority
- CN
- China
- Prior art keywords
- pressure
- myocardial cell
- culturing room
- nutrient solution
- set screw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a tissue engineering myocardial cell pulse pressure detecting device. The tissue engineering myocardial cell pulse pressure detecting device comprises three parts, namely a tissue engineering myocardial cell culturing room, a measuring unit and a micropressure regulating unit; the tissue engineering myocardial cell culturing room comprises a myocardial cell culturing room, a culturing observation room cover plate, a culture solution inlet, a culture solution outlet, a sealing plug and a clean air inlet; the measuring unit is composed of a first hose, an instrument fixing base and a pressure instrument; the micropressure regulating unit is composed of a second hose, a pressure applying air sac, an air vent valve, a first fixed screw, a fourth fixed screw, a fixed pressure plate, a pressure piston cylinder, a cylinder support frame, a support base plate, a pressure piston, a second fixed screw, a fixed support plate, a horizontal micrometer dial, a fixed support, a horizontal micro motion sliding table and a third fixed screw. The tissue engineering myocardial cell pulse pressure detecting device adopts a high-accuracy measurement instrument and is provided with a micropressure regulating device, so that the measured data is accurate and reliable.
Description
Technical field
The utility model belongs to biomedical engineering field, relates to the proofing unit that a kind of organizational project myocardial cell is beaten and pressed.
Background technology
Cell therapy to heart stalk and whole latter stage cardiac disorder treatment there are bright prospects, but current effect is clinically not bery desirable, its one of the main reasons is exactly that the myocardial cell's of vitro culture mechanical property is not mated with host cell, therefore the device of myocardial cell's mechanical characteristic is measured in research and development, and the application to cell therapy on clinical medicine is significant.
The most important function of myocardial cell be exactly pulsation be regular contractile motion, therefore measuring myocardial cell's mechanical characteristic most important is exactly to measure its pressures of beating---systolic pressure and diastolic pressure, but does not still measure the beat measuring apparatus of pressure of myocardial cell at present.
Summary of the invention
The purpose of this utility model is to provide a kind of size based on accurately changing pressure in myocardial cell culturing room, observes corresponding myocardial cell's the state of beating, and builds and measures the device that myocardial cell is beaten and pressed.This device can be to myocardial cell culturing room by the injection of uncontaminated air with eject the size of the pressure that regulates culturing room, then the situation of beating under different pressure is measured the size that it is beaten and presses by microscopic examination myocardial cell.When measuring, increase pressure, when observing culturing room's cardiac myocyte cut and beat, the shown value of tensimeter is exactly systolic pressure, then reduce pressure, to an instant of observing myocardial cell and beating again, the value that now tensimeter shows is exactly diastolic pressure.Because device has adopted high-precision metrical instrument, be provided with minute-pressure setting device, the data that record are accurately reliably.
The utility model comprises organizational project myocardial cell culturing room, measuring unit and three parts of minute-pressure regulon.
Organizational project myocardial cell culturing room comprises myocardial cell culturing room, cultivates observation room cover plate, nutrient solution import, nutrient solution outlet, sealing plug and uncontaminated air entrance.
The top of myocardial cell culturing room is transparent cultivation observation room cover plate, and the two ends of myocardial cell culturing room are respectively nutrient solution import and nutrient solution outlet, in nutrient solution outlet, are provided with sealing plug, the double uncontaminated air entrance of doing of nutrient solution import.
Measuring unit is comprised of the first flexible pipe, instrument firm banking, manometer.
The first soft pipe connection nutrient solution import, manometer is arranged on the pipeline of the first flexible pipe by instrument firm banking.
Minute-pressure regulon applies air bag, purging valve, the first set screw, the 4th set screw, fixation clip, pressure piston cylinder, cylinder supports frame, support baseboard, pressure piston, the second set screw, fixed support plate, horizontal micrometer, fixed support, horizontal fine motion slide unit, the 3rd set screw by the second flexible pipe, pressure and forms.
The second flexible pipe is by instrument firm banking and the first soft pipe connection; Pressure applies air bag and the second soft pipe connection, for culturing room is exerted pressure; Purging valve is positioned at pressure and applies on air bag and survey, the discharge of control pressure; Pressure piston cylinder is also hose connection with second; Fixation clip is fixed to pressure piston cylinder on cylinder supports frame by the first set screw and the 4th screw, and cylinder supports frame is connected with support baseboard again; The second set screw is fixed on one by pressure piston and fixed support plate; Horizontal micrometer is connected with horizontal fine motion slide unit by fixed support, and horizontal fine motion slide unit is connected with fixed support plate by the 3rd set screw again.
The utility model has been realized the measurement that myocardial cell is beaten and pressed first, simultaneously also can be by observe myocardial cell's number within the unit time, and then obtain its frequency.Its advantage is simple to operate, and suitability is good, safe and reliable, for biomedical engineering field organizational project myocardial cell's mechanical property measurement provides a kind of new method.
Accompanying drawing explanation
Fig. 1 is the utility model structural representation.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail.
As shown in Figure 1, a kind of myocardial cell's of measurement the device of pressing of beating mainly contains culturing room unit, and measuring unit, minute-pressure regulon 3 parts form.
Culturing room unit comprises sealing plug 1; Nutrient solution outlet 2; Uncontaminated air entrance (nutrient solution entrance) 22; Cultivate observation room cover plate 23; Myocardial cell culturing room 24; The 5th set screw 25.
Cultivating observation room cover plate 23 is connected by the 5th set screw 25Yu myocardial cell culturing room 24.Left surface in myocardial cell culturing room 24 has nutrient solution outlet 2, and there is sealing plug 1 in exit.At myocardial cell culturing room 24 right flanks, there is uncontaminated air entrance (nutrient solution entrance) 22.The effect of culturing room is to cultivate our required myocardial cell; It is transparent cultivating observation room cover plate, so that our beating by microscopic examination myocardial cell; The effect of nutrient solution outlet is to make nutrient solution flow out, and removes old nutrient solution; The effect Shi Dang culturing room pressure of sealing plug guarantees air tight while increasing; Thereby the effect of uncontaminated air entrance is to make air inlet and outlet reach the object that changes culturing room's pressure, and the input of nutrient solution is simultaneously also to complete on this mouthful, injects new nutrient solution.Whole myocardial cell cultivates reactor and seals, and has avoided the impact of bacterium in contact outside air.
Measuring unit comprises the first flexible pipe 3; Instrument firm banking 4; Manometer forms 5.
First flexible pipe 3 left sides connect uncontaminated air entrance (nutrient solution entrance) 22, and the right connects instrument firm banking 4; Manometer 5 is fixed on instrument firm banking 4.The first soft pipe connection culturing room, has guaranteed that the pressure of flexible pipe and the pressure of culturing room are consistent; The effect of instrument firm banking is fixation pressure instrument; Manometer can be measured the pressure size on the flexible pipe at place accurately.
Minute-pressure regulon comprises that the second flexible pipe 6, pressure apply air bag 21, purging valve 20, the first set screw 7, the 4th set screw 18, fixation clip 8, pressure piston cylinder 9, cylinder supports frame 19, support baseboard 14, pressure piston 10, the second set screw 11, fixed support plate 12, horizontal micrometer 13, fixed support 15, horizontal fine motion slide unit 16, the 3rd set screw 17 compositions.
The second flexible pipe 6 is communicated with the first flexible pipe 3 by instrument firm banking 4; Pressure applies air bag 21 and is communicated with the second flexible pipe 6; Purging valve 20 is positioned at pressure and applies survey on air bag 21, the discharge of control pressure; Pressure piston cylinder 9 is connected with the second flexible pipe 6; Fixation clip 8 is fixed to pressure piston cylinder 9 on cylinder supports frame 19 by the first set screw 7 and the 4th set screw 18, and cylinder supports frame 19 is connected with support baseboard 14 again; The second set screw 11 is fixed on one by pressure piston 10 and fixed support plate 12; Horizontal micrometer 13 is connected with horizontal fine motion slide unit 16 by fixed support 15, and horizontal fine motion slide unit 16 is connected with fixed support plate 12 by the 3rd set screw 17 again.Wherein the principle of horizontal micrometer and the principle of screw micrometer are similar, by rotarily driving the movement of horizontal fine motion slide unit, thereby promote piston, make cylinder produce micro-pressure.
Organizational project myocardial cell's measurement of pressing of beating specifically comprises the following steps:
1, culturing room is taken off separately, put into seed cell, nutrient solution entrance, nutrient solution outlet are connected with nutrient solution pipeline, inject nutrient solution, under sterile environment, put into incubator and cultivate myocardial cell.
2, turn out after myocardial cell and (by microscopes, observe the short cylinder cell of the feature of beating), culturing room is connected with measurement and micrometric displacement unit below.
3, close purging valve, press pressure applies air bag, to culturing room, pressurizes.
4, by microscopic examination, observe beating of myocardial cell when faint, stop by pressuring gasbag, use the horizontal micrometer of rotation instead, be rotated in the forward at leisure, until myocardial cell's the instant of beating and stopping.The value now showing on tensimeter is exactly systolic pressure.
5, opening purging valve slowly exits, observe myocardial cell slowly recovers to beat simultaneously, when beating when more obvious, close purging valve, the horizontal micrometer of reverse rotation, rotation limit, limit is observed, until myocardial cell beats the most obviously and rotates small distance while not changing again, the value that now tensimeter shows is diastolic pressure.
Claims (1)
1. the organizational project myocardial cell pressure proofing unit of beating, comprises organizational project myocardial cell culturing room, measuring unit and three parts of minute-pressure regulon, it is characterized in that:
Organizational project myocardial cell culturing room comprises myocardial cell culturing room, cultivates observation room cover plate, nutrient solution import, nutrient solution outlet, sealing plug and uncontaminated air entrance;
The top of myocardial cell culturing room is transparent cultivation observation room cover plate, and the two ends of myocardial cell culturing room are respectively nutrient solution import and nutrient solution outlet, in nutrient solution outlet, are provided with sealing plug, the double uncontaminated air entrance of doing of nutrient solution import;
Measuring unit is comprised of the first flexible pipe, instrument firm banking, manometer;
The first soft pipe connection nutrient solution import, manometer is arranged on the pipeline of the first flexible pipe by instrument firm banking;
Minute-pressure regulon applies air bag, purging valve, the first set screw, the 4th set screw, fixation clip, pressure piston cylinder, cylinder supports frame, support baseboard, pressure piston, the second set screw, fixed support plate, horizontal micrometer, fixed support, horizontal fine motion slide unit, the 3rd set screw by the second flexible pipe, pressure and forms;
The second flexible pipe is by instrument firm banking and the first soft pipe connection; Pressure applies air bag and the second soft pipe connection, for culturing room is exerted pressure; Purging valve is positioned at pressure and applies on air bag and survey, the discharge of control pressure; Pressure piston cylinder is also hose connection with second; Fixation clip is fixed to pressure piston cylinder on cylinder supports frame by the first set screw and the 4th screw, and cylinder supports frame is connected with support baseboard again; The second set screw is fixed on one by pressure piston and fixed support plate; Horizontal micrometer is connected with horizontal fine motion slide unit by fixed support, and horizontal fine motion slide unit is connected with fixed support plate by the 3rd set screw again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420045380.6U CN203768361U (en) | 2014-01-24 | 2014-01-24 | Tissue engineering myocardial cell pulse pressure detecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420045380.6U CN203768361U (en) | 2014-01-24 | 2014-01-24 | Tissue engineering myocardial cell pulse pressure detecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203768361U true CN203768361U (en) | 2014-08-13 |
Family
ID=51285572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420045380.6U Withdrawn - After Issue CN203768361U (en) | 2014-01-24 | 2014-01-24 | Tissue engineering myocardial cell pulse pressure detecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203768361U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103805505A (en) * | 2014-01-24 | 2014-05-21 | 杭州电子科技大学 | Device and method for detecting pulsating pressure of tissue engineering myocardial cell |
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
-
2014
- 2014-01-24 CN CN201420045380.6U patent/CN203768361U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103805505A (en) * | 2014-01-24 | 2014-05-21 | 杭州电子科技大学 | Device and method for detecting pulsating pressure of tissue engineering myocardial cell |
| CN103805505B (en) * | 2014-01-24 | 2015-07-29 | 杭州电子科技大学 | Organizational project myocardial cell is beaten and is pressed proofing unit and method |
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
| CN116286315B (en) * | 2023-03-21 | 2023-10-27 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105030214B (en) | A kind of intelligence is felt the pulse analyzer | |
| CN103271720B (en) | A kind of adjustable electropulsograph of ram position of measuring | |
| CN103805505B (en) | Organizational project myocardial cell is beaten and is pressed proofing unit and method | |
| CN203768361U (en) | Tissue engineering myocardial cell pulse pressure detecting device | |
| CN203943659U (en) | A kind of portable wound arteriotony monitoring device that has | |
| CN203953765U (en) | A kind of intrathecal injection lumbar puncture needle | |
| CN104480009A (en) | Bioreactor device with double functions of perfusion and tensioning | |
| CN211094046U (en) | Pulse condition acquisition equipment based on high-definition high-speed camera shooting technology | |
| CN207502096U (en) | A set of mechanics parameter for engineering blood vessel culture monitors system | |
| CN104120083B (en) | A kind of organism culturing device | |
| CN108378873A (en) | A kind of Neurology clinic liquid taking device | |
| CN206609633U (en) | A kind of preclinical medicine check sampling device | |
| CN208640721U (en) | A kind of blood testing blood sample collecting device | |
| CN204311067U (en) | A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor | |
| CN204039417U (en) | A kind of organism culturing device | |
| CN202024944U (en) | Multi-passage blood viscosity measuring device | |
| CN220625664U (en) | Disposable sterile injection needle air tightness detection device | |
| CN207804243U (en) | A kind of electronic blood pressure instrument meter easy to use | |
| CN103976722A (en) | Non-invasive pressure measuring method for esophageal varices | |
| CN203953637U (en) | A kind of novel electron survey meter of blood pressure | |
| CN215162717U (en) | Portable blood bacteria tester | |
| CN109668883A (en) | A kind of Detecting Pesticide pen | |
| CN104388311A (en) | Bioreactor for culturing and measuring tissue engineering stem cell-induced myocardial cells | |
| CN205252090U (en) | Traditional chinese medicine fermentation bag | |
| CN203493672U (en) | Dermatophyte inspection tool |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140813 Effective date of abandoning: 20150729 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20140813 Effective date of abandoning: 20150729 |
|
| RGAV | Abandon patent right to avoid regrant |