CN105640533A - In-vitro hemodynamic characteristic test device - Google Patents

Abstract

An in vitro hemodynamic characteristic test device, mainly including a constant temperature control box, a peristaltic pump, a pressure sensor, a non-contact microvelocity measurement system, etc.; the constant temperature control box is connected by pipelines and forms a closed loop. There is simulated blood containing tracer particles, the outlet of the constant temperature water tank passes through the peristaltic pump through the silicone hose, and the transparent elastic hose of the test area is connected to the inlet of the constant temperature water tank; before and after the test area, the silicone hose and the transparent elastic hose of the test area Valves are installed on the joints, and the transparent elastic hose in the test area is connected to the silicone hose through a silicone joint; a pressure sensor and a non-contact microscopic velocity measurement system are installed at the test section of the transparent elastic hose in the test area. The device of the present invention can provide continuous simulated pulsating blood flow, has a hemodynamic characteristic parameter testing system, and can test hemodynamic characteristic parameters in different blood vessels, such as blood flow velocity, wall pressure, wall shear stress and the like.

Description

An in vitro hemodynamic characteristic testing device

technical field

The invention belongs to the technical field of biomedicine and measurement and control, and relates to an in vitro hemodynamic characteristic testing device.

Background technique

The shape and diameter of blood vessels in different parts of the human body are not the same, and the diameter of blood vessels may also change at different ages in the same individual. In the human vascular system, the main hemodynamic parameters are: blood flow velocity, wall shear force, wall pressure, etc. Among them, blood flow velocity, wall shear force and wall pressure represent blood flow, blood flow resistance and blood pressure, respectively.

It is difficult to directly test the hemodynamic parameters of blood vessels in the human body due to the harsh experimental environment and conditions. Compared with the in vivo test, the in vitro test system has strong operability and high controllability, and is applied in the field of modern biomedical research and clinical medical examination. The in vitro hemodynamic characteristic test device uses the principle of flow similarity to simulate the blood flow of the human body, and obtains the dynamic characteristic parameters of the simulated blood flow through measurement and control technology.

Blood flow has its own characteristics. To realize in vitro blood flow test and establish a set of in vitro hemodynamic characteristics test system, it is necessary to have simulated blood vessels and continuous pulsating simulated blood flow. The peristaltic pump can provide the simulation of heart beat. Modern manufacturing technology can prepare simulated blood vessels, and obtain the hemodynamic characteristic parameters of simulated blood through data collection and particle velocity measurement.

Contents of the invention

The purpose of the present invention is to provide a hemodynamic characteristic testing device in vitro, which can provide continuous simulated pulsating blood flow, has a hemodynamic characteristic parameter testing system, and can test hemodynamic characteristic parameters in different blood vessels , such as blood flow velocity, wall pressure, wall shear stress, etc.

The present invention is realized through the following technical solutions.

An in vitro hemodynamic characteristic testing device according to the present invention, comprising a constant temperature control box (1), a peristaltic pump (2), a silicone hose (4), a non-contact microvelocimetry system (7), Pressure sensor (11), transparent elastic hose (8) in the test area, etc. The thermostatic control box (1) is connected by pipelines to form a closed loop. The thermostatic control box (1) is filled with simulated blood (2) containing tracer particles, and the outlet of the thermostatic control box (1) is passed through a silicone hose (4 ) through the peristaltic pump (3), and then connected to the inlet of the thermostatic control box (1) through the transparent elastic hose (8) in the test area; before and after the test area, the silicone hose (4) and the transparent elastic hose ( 8) Valve 1 (5) and valve 2 (10) are installed at the connection respectively, and the transparent elastic hose (8) in the test area passes through the inlet silicone joint (6) and the outlet silicone joint (9) of the test area and the silicone hose (4) )connect. A pressure sensor (11) is installed at the test position of the transparent elastic hose (8) in the test area, which is used to measure the wall pressure of the transparent elastic hose (8) in the test area, which is realized by a high-speed data acquisition card (13) and a computer (16). Data collection; the test section of the transparent elastic hose (8) in the test area is also equipped with a non-contact microvelocity measurement system (7) for capturing particle images in the test section of the transparent elastic hose (8) in the test area. Among them, the non-contact microscopic velocity measurement system (7) is composed of an industrial high-speed camera (21), an industrial microscope lens (22), and a ring-shaped LED light source (23). The industrial high-speed camera (21), the industrial microscope lens (22), and the ring-shaped LED light source (23) are connected with precision threads. When measuring the wall surface pressure, install the transparent elastic hose (8) in the test area with micropores, and install the transparent elastic hose (8) in the test area without micropores when measuring the blood flow velocity.

The temperature of the constant temperature control box (1) can be manually set to keep the simulated blood temperature within the actual normal blood temperature range, and the digital display of the constant temperature control box (1) displays the temperature. The speed adjustment of the peristaltic pump (3) can be manually controlled or automatically adjusted through an external control interface. The speed adjustment range is 60~100r/min. The pump tube bed passes between the racks, and the diameter of the silicone hose (4) can be selected according to the experimental requirements.

The transparent elastic hose (8) in the test area is used as a simulated blood vessel, and is fixed on the white liner (18). ) can be installed and disassembled according to requirements. The transparent elastic hose (8) in the test area has good elasticity and corrosion resistance, and has good light transmission, which will not affect the flow field observation. When measuring the wall surface pressure, install a transparent elastic hose with micro-holes, which match the pressure sensor probe. The micro-holes can be set at any position in the test area of the transparent elastic hose. When measuring blood flow velocity, install without micro-holes transparent elastic hose.

The inlet silicone connector (6) and outlet silicone connector (9) of the test area can be installed and replaced according to requirements, but they need to match the silicone hose (4) and the transparent elastic hose (8) of the test area according to the test conditions .

The simulated blood is PSB simulated body fluid, which is a transparent working medium and has similar ion environment and similar kinematic viscosity to human blood.

The pressure sensor (11) has a probe with high sensitivity and certain strength, the diameter of which is on the order of microns, matches the micropores in the test area of the transparent elastic hose, and does not leak liquid. It can also be replaced with measurement sensors of other parameters according to test requirements.

The diameter of tracer particles in the simulated blood can be as low as several micrometers, and has better visibility and flow followability.

The non-contact microscopic velocimetry system (7) can capture clear particle images, and it is composed of an industrial high-speed camera (21), an industrial microscope lens (22), an annular LED light source (23) and a fixed bracket (24). The industrial high-speed camera (21) is a high-frame rate camera, the industrial microscope lens (22) is a high-power microscope, and the ring-shaped LED light source (23) has stable light intensity, anti-interference, no reflection, and adjustable brightness. Fixed bracket (24) is adjustable and flexible.

Beneficial effects of the present invention.

1) The test device of the present invention simulates the beating of the heart through the peristaltic function of the peristaltic pump, controls the extrusion of the silicone hose by the peristaltic pump, and generates continuous pulsating fluid, which can effectively simulate the blood flow state in the blood vessel; the test area is transparent The choice of elastic hose has a certain degree of flexibility, and can be made into simulated blood vessels of required shape and diameter according to test requirements.

2) The test device of the present invention can obtain the hemodynamic characteristic parameters in the simulated blood vessel, and the wall pressure of the simulated blood vessel can be measured by using the pressure sensor; the particle image in the simulated blood vessel is obtained by using the non-contact microvelocimetry system, and the particle image in the simulated blood vessel is obtained by the post-processing software. The flow field data is analyzed and processed to obtain information such as intravascular blood flow velocity, wall shear stress, and wall pressure.

3) The test device of the present invention can also test other hemodynamic parameters, such as replacing the pressure sensor with a flow sensor to measure blood flow and the like.

Description of drawings

Fig. 1 is a schematic diagram of an in vitro hemodynamic characteristic testing device of the present invention.

Fig. 2 is a structural schematic diagram of the peristaltic pump and the pump head in the device for testing the hemodynamic characteristics in vitro of the present invention.

Fig. 3 is a schematic diagram of the transparent elastic tube in the testing area in the in vitro hemodynamic characteristic testing device of the present invention.

Fig. 4 is a schematic diagram of the non-contact microvelocimetry system in the in vitro hemodynamic characteristic testing device of the present invention.

In Figures 1 to 4: 1 is the constant temperature control box, 2 is the simulated blood containing tracer particles, 3 is the peristaltic pump, 4 is the silicone hose, 5 is the valve 1, 6 is the silicone connector at the entrance of the test area, and 7 is the non-contact 8 is the transparent elastic hose in the test area, 9 is the silicone joint at the outlet of the test area, 10 is the valve 2, 11 is the pressure sensor, 12 is the data communication line of the pressure sensor, 13 is the high-speed data acquisition card, 14 is Communication line, 15 is a computer, 16 is an industrial high-speed camera communication line, 17 is a communication line, 18 is a white liner, 19 is a peristaltic pump head frame, 20 is a peristaltic pump head rotor, 21 is an industrial camera, and 22 is an industrial microscope Head, 23 is an annular LED light source, and 24 is a fixed bracket.

detailed description

The specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

Referring to Fig. 1, the in vitro hemodynamic characteristic testing device of the present invention comprises a constant temperature control box 1, a peristaltic pump 3, a silicone hose 4, a non-contact microvelocimetry system 7, a transparent elastic hose 8 in a test area, and a pressure sensor 11 . The piping of the whole device is connected in this way: the silicone hose 4 is connected to the thermostatic control box 1, one end of which passes through the pump head of the peristaltic pump 3, and one end of the silicone joint 6 at the inlet of the test area and the transparent elastic hose 8 of the test area Connected, the other end of the transparent elastic hose 8 in the test area is connected with the silicone joint 9 at the outlet of the test area, and the silicone joint 9 at the outlet of the test area is connected with the other end of the silicone hose 4, and the valve 1 5 and valve 2 10 are respectively installed in the test area The connection between the transparent elastic hose 8 and the two ends of the silicone tube 4, and finally the silicone hose 4 is connected to the inlet of the thermostatic control box 1. Since the tube wall of the transparent elastic hose 8 in the test area is a colorless and transparent curved surface, and the non-contact microvelocimetry system 7 needs to collect stable and clear images, in order to avoid the image shift caused by the vibration of the pipeline, the transparent elastic hose in the test area 8 is fixed on the white liner 18, the liner 18 is pure white with a smooth surface, the flow velocity of the simulated blood 2 containing the tracer particles is characterized by the velocity of the tracer particles, the transparent elastic hose 8 and the white liner 18 in the test area These qualities enhance the resolution of captured particle images. Install the pressure sensor and other test devices to ensure that the pipeline seal is not loose, then add the simulated blood 2 containing tracer particles into the constant temperature control box 1, set the temperature first, and automatically enter the constant temperature state when heated to the set temperature. Start the peristaltic pump 3, and after the simulated blood in the pipeline forms a pulsating flow, the relevant parameters can be tested.

Referring to Fig. 2, the peristaltic pump 3 used in the present invention can manually adjust the rotating speed and steering, and can also be controlled by a computer through a communication line, and the digital display shows the current rotating speed. The peristaltic pump head of the peristaltic pump 3 is composed of two parts, namely the peristaltic pump head rotor 20 and the peristaltic pump head frame 19 . The pump tube bed is fixed between the peristaltic pump head rotor 20 and the peristaltic pump head frame 19. During the test, the silicone hose 4 is installed in the pump tube bed, pressed tightly with a gland, and the required parameters of the peristaltic pump 2 are set and When starting, the rotor 19 of the peristaltic pump head interacts with the silicone hose 4 to generate pulsating fluid. The invention studies the rules of blood flow in different blood vessels, tests its hemodynamic characteristics, provides continuous pulsating simulated blood flow for the whole test device through the peristaltic pump 3, and uses the rotational speed of the peristaltic pump 3 to represent the heart rate of a person. The rotating speed range of the peristaltic pump 3 used in the present invention is 0 ~ 300r/min, and the normal heart rate range of people is about 60 ~ 100 beats/min. In order to make the experimental results credible, in the test process, the rotating speed of the peristaltic pump 3 is set to A fixed value, and the fixed value is within the normal heart rate range.

Referring to Fig. 3, in the in vitro hemodynamic characteristic testing device of the present invention, different transparent elastic hoses can be made as simulated blood vessels according to the test requirements, as shown in Fig. 3, which is a schematic diagram of transparent elastic hoses in different test areas, but in the device of the present invention The test area of the transparent elastic hose is by no means limited to the schematic diagram in Figure 3. In this test device, transparent elastic hoses of different shapes and diameters in the test area can be prepared according to the test requirements, and the simulated blood vessels to be tested can be drawn by using the drawing software, and then made by precision instruments. During the test, when measuring the wall pressure through the data acquisition system, first connect the transparent elastic hose in the test area with micropores to the silicone tube, and then connect the pressure sensor probe to the transparent elastic hose in the test area with micropores to measure the wall pressure. The position of the pressure is not fixed, and the microhole can be set in any test area of the transparent elastic hose in the test area according to the test requirements; the connected sensor is not limited to the pressure sensor, and can be replaced with a flow sensor to measure other blood flow parameters such as flow ; When measuring the velocity of the simulated blood flow, first connect the non-microporous transparent elastic hose to the silicone tube, and then align the speed measurement system to the area to be measured. When replacing the transparent elastic hose in the test area, stop the operation of the peristaltic pump first, close the valve on the silicone tube to prevent fluid from flowing out, then replace the inlet and outlet silicone connectors that match the transparent elastic hose in the test area, and finally connect the transparent elastic tube in the test area hose.

Referring to FIG. 4 , the non-contact microvelocity measurement system 7 in the present invention includes an industrial high-speed camera 21 , an industrial microscope lens 22 , an annular LED light source 23 , and a fixed bracket 24 . The non-contact microvelocimetry system 7 is used to test the flow of simulated blood 2 containing tracer particles. Start the peristaltic pump 3 during the test. After the simulated blood in the pipeline forms a pulsating flow, first fix the industrial high-speed camera 21 on the fixed bracket 24, then install the industrial microscope lens 22, and then fix the ring LED light source 23 on the microscope lens. Align the velocity measurement area of the transparent elastic hose 8 in the test area, adjust the brightness of the industrial microscope lens 22, the ring LED light source 23 and the parameters of the industrial high-speed camera 21 until the tracer particles in the measured area of the transparent elastic hose are clearly visible, and then The particle image is captured by the image capture software, and finally processed by the image control software of the computer to obtain the particle velocity and wall shear stress. In addition, particle image processing can be performed to obtain particle streamlines, and the distribution of particles in simulated blood vessels can also be observed.

Part of the silicone hose, pressure sensor, transparent elastic hose in the test area and non-contact microvelocity measurement system in the present invention are all placed on the white liner to avoid the vibration of the peristaltic pump and other external factors affecting the measurement of parameters and capturing particles The resolution of the image should not be measured at the same time as the wall pressure and blood flow velocity, because the measurement of blood flow velocity may be interfered during the wall pressure test.

Claims (8)

1. an extracorporeal blood flow dynamic property test device, is characterized in that comprising thermostatically controlled tank (1), peristaltic pump (2), silica gel hose (4), the micro-velocity-measuring system of noncontact formula (7), pressure transmitter (11), test zone transparent elastic flexible pipe (8); Thermostatically controlled tank (1) is connected by pipeline, and form closed loop, thermostatically controlled tank (1) is built with the simulate blood (2) containing trace particle, the outlet of thermostatically controlled tank (1) is through silica gel hose (4) through peristaltic pump (3), then district's transparent elastic flexible pipe (8) is connected with the entrance of thermostatically controlled tank (1) after tested; The front and back of test zone, silica gel hose (4) and transparent elastic flexible pipe (8) junction, test zone are separately installed with valve one (5) and valve two (10), and test zone transparent elastic flexible pipe (8) is connected with silica gel hose (4) by test zone entrance silica gel joint (6) and outlet silica gel joint (9); The test position of test zone transparent elastic flexible pipe (8) is provided with pressure transmitter (11), for measuring the wall pressure of test zone transparent elastic flexible pipe (8), realizes data gathering by high-speed data acquisition card (13) and computer (16); The test section of test zone transparent elastic flexible pipe (8) is also provided with the micro-velocity-measuring system of noncontact formula (7), is used for the particle picture caught in transparent elastic flexible pipe (8) test section of test zone; The micro-velocity-measuring system (7) of wherein noncontact formula is made up of industry high speed camera (21), industrial microscope head (22), annular LED light source (23); It is accurate thread between industry high speed camera (21), industrial microscope head (22), annular LED light source (23) to connect; When surveying wall pressure, test zone transparent elastic flexible pipe (8) of micropore is installed, when surveying blood flow rate, then test zone transparent elastic flexible pipe (8) of pore-free is installed.

2. extracorporeal blood flow dynamic property test device according to claim 1, it is characterized in that described thermostatically controlled tank (1) temperature is by artificial setting, simulate blood temperature is made to remain in the actual normal temperature range of blood, the digital display meter displays temperature of thermostatically controlled tank (1);The speed adjustment of described peristaltic pump (3) can also regulate by outer control interface mode by Non-follow control automatically, speed adjustable range is 60 ~ 100r/min, the pump line bed of silica gel hose (4) between the pump head rotor and frame of peristaltic pump (3) passes, and the diameter of silica gel hose (4) can experimentally need to ask and selects.

3. extracorporeal blood flow dynamic property test device according to claim 1, it is characterized in that described test zone transparent elastic flexible pipe (8) is as simulated blood vessel, it is fixed in white liner plate (18), liner plate should have certain intensity, the weight being enough to bear put instrument, test zone transparent elastic flexible pipe (8) can mount and dismount according to demand, and test zone transparent elastic flexible pipe (8) possesses good elasticity and erosion resistance, and light transmission is good, does not affect flow field and observe; When surveying wall pressure, installing the transparent elastic flexible pipe with micropore, micropore mates mutually with pressure transmitter probe, and micropore can be arranged on the optional position of transparent elastic flexible pipe test zone, when surveying blood flow rate, installs the transparent elastic flexible pipe of pore-free.

4. extracorporeal blood flow dynamic property test device according to claim 1, it is characterized in that described test zone entrance silica gel joint (6) and outlet silica gel joint (9) can be installed as requested and replace, but they need to mate mutually with silica gel hose (4) and test zone transparent elastic flexible pipe (8) according to test condition.

5. extracorporeal blood flow dynamic property test device according to claim 1, is characterized in that described simulate blood is PSB simulated body fluid, is transparent operation medium, have the ionic environment similar to blood of human body and close kinematic viscosity.

6. extracorporeal blood flow dynamic property test device according to claim 1, it is characterized in that described pressure transmitter (11) its sensor sensitivity is very high, have certain intensity, diameter is micron dimension, mate mutually with the micropore of transparent elastic flexible pipe test zone, no leakage; The survey sensor of other parameters is also can be replaced according to test demand.

7. extracorporeal blood flow dynamic property test device according to claim 1, the trace particle diameter that it is characterized in that in described simulate blood can be low to moderate several microns, has visual preferably and flowing followability.

8. extracorporeal blood flow dynamic property test device according to claim 1, it is characterized in that the described micro-velocity-measuring system of noncontact formula (7) can catch particle picture clearly, it is made up of industry high speed camera (21), industrial microscope head (22), annular LED light source (23) and fixing support (24); Described industry high speed camera (21) is high frame per second camera, and industrial microscope head (22) is powerful microscope, and annular LED light source (23) light intensity is stable, anti-interference, no-reflection, and brightness is adjustable; Fixing support (24) is adjustable and handiness good.