CN112592812B - Inoculation device for muscle cell culture - Google Patents
Inoculation device for muscle cell culture Download PDFInfo
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- CN112592812B CN112592812B CN202011480270.9A CN202011480270A CN112592812B CN 112592812 B CN112592812 B CN 112592812B CN 202011480270 A CN202011480270 A CN 202011480270A CN 112592812 B CN112592812 B CN 112592812B
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- 238000011081 inoculation Methods 0.000 title claims abstract description 67
- 238000004113 cell culture Methods 0.000 title claims abstract description 10
- 210000000663 muscle cell Anatomy 0.000 title claims abstract description 8
- 210000003811 finger Anatomy 0.000 claims description 73
- 210000005224 forefinger Anatomy 0.000 claims description 20
- 239000013013 elastic material Substances 0.000 claims description 9
- 210000004932 little finger Anatomy 0.000 claims description 9
- 230000009471 action Effects 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 210000003813 thumb Anatomy 0.000 description 17
- 210000001519 tissue Anatomy 0.000 description 14
- 239000002245 particle Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000002775 capsule Substances 0.000 description 4
- 210000001503 joint Anatomy 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- 238000013016 damping Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 208000003443 Unconsciousness Diseases 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 210000003437 trachea Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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- Life Sciences & Earth Sciences (AREA)
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- Sustainable Development (AREA)
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- Biotechnology (AREA)
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Abstract
The invention discloses an inoculation device for muscle cell culture, which relates to the field of cell culture equipment. The invention has the beneficial effects that: this scheme is through changing traditional inoculation instrument form, has adopted the structure that more accords with the blake bottle inoculation operation, and with the help of inciting somebody to action inoculation apparatus and each finger carry out the relevance, can be more convenient for extend to the blake bottle with the inoculation pipe in and operate.
Description
Technical Field
The invention relates to the field of cell culture equipment, in particular to an inoculation device for muscle cell culture.
Background
In muscle cell culture, after muscle tissue is processed into tissue particles, the tissue particles are inoculated into a culture flask or a culture dish. The means for inoculation is typically an ophthalmic forceps, pipette or inoculating loop. For the inoculation vessel, a petri dish and a culture flask are common. For above-mentioned inoculation instrument, the culture dish is because can remove the dish lid completely, convenient operation during the inoculation, and with tissue grain dispersion arrangement in the culture dish during the inoculation, need wait for a certain time so that the tissue adherence, need open the culture dish again after the adherence is accomplished and add the nutrient solution, this in-process can lead to histiocyte to receive the bacterium influence. To this problem, the blake bottle can fine solution, through its structure, inoculation and nutrient solution's injection can accomplish simultaneously, only need wait for tissue adherence after with the blake bottle rotatory, alright make nutrient solution and tissue contact, need not open the bottle lid in this process. However, due to the structural limitation of the culture bottle, when the traditional inoculating tool inoculates the culture bottle, the inoculating operation is very inconvenient because the mouth of the culture bottle is small.
Disclosure of Invention
In order to solve the technical problems, the invention provides an inoculation device for muscle cell culture.
Its technical scheme does, locate the forefinger external member on user's forefinger including the cover, and the forefinger external member is including being used for connecting ring portion with the finger, ring portion upside connecting line portion, and the one end that pipeline portion is close to the fingertip is connected the inoculation pipe, and the other end passes through the trachea and is connected with the air pump, inoculation pipe, pipeline portion, trachea and the inside intercommunication of air pump.
Preferably, the finger ring part of the index finger kit comprises three fixing rings, the finger tip part, the middle part and the palm finger part of the index finger respectively correspond to the finger tip part, the middle part and the palm finger part of the index finger, the upper side of each fixing ring is fixedly connected with a pipe frame, the pipe frame comprises two semi-circular arc-shaped hoop bodies, the two hoop bodies are connected through bolts, the lower sides of the hoop bodies are fixedly connected with the fixing rings, the upper sides of the hoop bodies are fixedly provided with connecting plates, and bolt holes are formed in the connecting plates;
the pipeline part comprises a movable pipe, each knuckle of the movable pipe, corresponding to the forefinger, is divided into a fingertip pipe, a middle pipe and a metacarpophalangeal pipe, and the fingertip pipe, the middle pipe and the metacarpophalangeal pipe are respectively sleeved with pipe frames of the fingertip part, the middle part and the metacarpophalangeal part, corresponding to the fixing rings;
the end of the fingertip tube, which is far away from the palm, is connected with the inoculation tube, the end of the metacarpophalangeal tube, which faces the palm, is connected with the air tube, and the two ends of the middle tube are respectively connected with the fingertip tube and the metacarpophalangeal tube through connecting tubes; the connecting pipe comprises a straight pipe body positioned in the middle, ball blocks are arranged at two ends of the straight pipe body respectively, the connecting pipe is hollow, and the connecting pipe is connected with the fingertip pipe, the middle pipe and the palm fingertip pipe through the ball blocks in a ball hinge mode.
Preferably, the hoop body of the pipe frame is made of elastic material.
Preferably, the ball block and the joint of the fingertip tube, the middle tube and the palm and finger tube with the ball block are all made of elastic materials, such as PC soft glue. Select for use the elastic material as connection structure, under the prerequisite of guaranteeing mobility, through appropriate interference fit, can make the junction from taking good damping force, can not lead to the inoculation pipe to rock because of the slight action of finger unconsciousness.
Preferably, the connection parts of the fingertip tube, the middle tube and the palm-finger tube and the ball block are provided with annular elastic cushion rings.
Preferably, the air pump comprises a bag body made of elastic materials, a cushion block is fixedly arranged on one side of the air pump, and a double-finger ring corresponding to the ring finger and the little finger is fixedly arranged on the other side of the air pump;
the bag body is provided with an air nozzle which is communicated with the air pipe.
Preferably, the through-hole has been seted up to two finger rings inside, and one side that the utricule was kept away from to the through-hole sets up the arcwall face of two evaginations, and this design more accords with human self structure, improves the comfort level of operation.
When the air pump is used, an operator holds the bag body of the air pump in the palm, the ring finger and the little finger are placed in the two-finger ring, the cushion block is attached to the palm, the suction action of the air pump can be realized by the opening of the ring finger and the little finger, the air pump sucks air, the suction force is transmitted to the pipeline part through the air pipe due to the air suction of the air pump, the inoculation pipe is accordingly sucked, and tissue particles are sucked by the inoculation pipe through the action. After the tissue pellet was aspirated, the inoculation tube was inserted into a culture flask. The inoculation tube is driven to search for a proper inoculation position in the culture bottle through the movement of the forefinger, then the capsule body is pressed by the ring finger and the little finger, and the tissue particles are inoculated on the inner wall of the culture bottle through air pressure.
Because the using-mode restriction of traditional inoculation straw, the user need grip the inoculation straw and just can conveniently absorb and place the tissue grain, so when the inoculation bottle in the face, then be difficult to the operation, this scheme realizes only controlling with forefinger butt joint seed tube position through the forefinger external member, when using the blake bottle as culture vessel, the operation accords with human custom more.
Preferably, the inoculation tube comprises a main tube communicated with the fingertip tube, and the main tube is coaxial with the fingertip tube after the inoculation tube is connected with the fingertip tube;
the one end that is kept away from the finger tip pipe of being responsible for sets up the pipe chute of tilt up, and the one end that is kept away from the person in charge of pipe chute sets up the plain tube, the axis of plain tube with the axis of being responsible for is parallel, the one end that is kept away from the person in charge of plain tube sets up the front end pipe, the front end pipe is buckled downwards.
Considering that the bottle mouth of the culture bottle is generally smaller and the bottle mouth has a certain inclination, the inoculation tube can be more conveniently inserted into the culture bottle to perform inoculation operation through the structure of the inoculation tube.
Preferably, the fingertip tube is connected with the inoculation tube through a rotary drum, the rotary drum is a cylinder, one end of the rotary drum is rotatably connected with the fingertip tube, and the other end of the rotary drum is inserted into the end part of the inoculation tube; connecting rings are symmetrically arranged on two sides of the drum body of the rotary drum;
the device also comprises a traction external member, wherein the traction external member is divided into a thumb external member and a middle finger external member which correspond to a thumb and a middle finger, the thumb external member and the middle finger external member respectively comprise two lantern rings which are arranged in parallel, the two lantern rings are fixedly connected through a connecting rod arranged on one side, and one end of the connecting rod, which faces to the fingertip, is fixedly provided with a pull ring;
the two connecting rings of the rotary drum are respectively connected with a pull ring of a middle finger sleeve of the thumb sleeve through a pull rope.
Preferably, the connecting rod is an elastic rod body, the two lantern rings of the thumb suite respectively correspond to the palm part and the fingertip part of the thumb, and the two lantern rings of the middle finger suite respectively correspond to the fingertip part and the middle part of the middle finger.
Preferably, the pull cord is made of a material having a slight elasticity.
During the use, wear thumb external member and middle finger external member respectively, at the inoculation in-process, when forefinger drove the inoculation pipe and does not obtain suitable position, can pass through thumb or middle finger pulling stay cord, make the rotary drum rotate to make the inoculation pipe carry out the rotation of certain angle, so that it is more convenient to operate in the blake bottle.
Preferably, wing plates are symmetrically arranged on two sides of the end part of the inoculation pipe, slots are arranged in the rotary drum corresponding to the wing plates, and the rotary drum is connected with the inoculation pipe in an inserted mode through the slots and the wing plates.
Preferably, the plate body of the wing plate is provided with a concave hole, and a spring ball is arranged in the slot of the rotary drum corresponding to the concave hole.
The flange plate is combined with the concave hole and the spring bead, so that the inoculation tube and the fingertip tube can be quickly disassembled and assembled.
Preferably, limiting plates are symmetrically and fixedly arranged on the upper side and the lower side of the finger tip portion, and wire passing holes are formed in the limiting plates and correspond to the pull ropes.
During the use, the stay cord that corresponds the both sides go-between winds the upper and lower both sides of rotary drum respectively around to different limiting plate department and pass the line hole and be connected with the pull ring, can avoid interfering mutually between two stay cords when the pulling to influence the rotary drum and rotate.
The technical scheme provided by the embodiment of the invention has the following beneficial effects: this scheme is through changing traditional inoculation instrument form, has adopted the structure that more accords with the blake bottle inoculation operation, and with the help of inciting somebody to action inoculation apparatus and each finger carry out the relevance, can be more convenient for extend to the blake bottle with the inoculation pipe in and operate.
Drawings
Fig. 1 is a schematic overall structure diagram of an embodiment of the present invention.
Fig. 2 is a partially enlarged view a of fig. 1.
Fig. 3 is a schematic structural diagram of a connection tube according to an embodiment of the invention.
Fig. 4 is a schematic structural diagram of an air pump according to an embodiment of the present invention.
Fig. 5 is a schematic view of a structure of a seed tube according to an embodiment of the invention.
FIG. 6 is a front view of a seed tube configuration according to an embodiment of the present invention.
Fig. 7 is a partially enlarged view a of fig. 2.
Wherein the reference numerals are: 100. a culture bottle; 1. an index finger assembly; 11. a finger ring part; 111. a fixing ring; 112. a pipe frame; 12. a pipe section; 121. a fingertip tube; 122. an intermediate pipe; 123. a palm-finger tube; 124. a connecting pipe; 125. a limiting plate; 2. inoculating a pipe; 21. a horizontal tube; 22. an inclined tube; 23. flattening the pipe; 24. a front end tube; 3. an air tube; 4. an air pump; 41. a capsule body; 42. cushion blocks; 43. a double finger ring; 5. a rotating drum; 51. a connecting ring; 6. pulling the sleeve member; 61. a collar; 62. a connecting rod; 63. a pull ring; 7. and pulling a rope.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. Of course, the specific embodiments described herein are merely illustrative of the invention and are not intended to be limiting.
It should be noted that the embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings, which are merely for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be construed as limiting the invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, the meaning of "a plurality" is two or more unless otherwise specified.
In the description of the invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "connected" and "disposed" are to be construed broadly, e.g. as being fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the creation of the present invention can be understood by those of ordinary skill in the art through specific situations.
Example 1
Referring to fig. 1 to 3, the present invention provides an inoculating device for muscle cell culture, comprising a forefinger kit 1 sleeved on a forefinger of a user, wherein the forefinger kit 1 comprises a finger ring part 11 for connecting with a finger, a pipeline part 12 is connected on the upper side of the finger ring part, one end of the pipeline part 12 close to a fingertip is connected with a seed tube 2, the other end is connected with an air pump 4 through an air tube 3, and the seed tube 2, the pipeline part 12, the air tube 3 and the air pump 4 are communicated with each other.
The finger ring part 11 of the forefinger kit 1 comprises three fixing rings 111 which respectively correspond to a finger tip part, a middle part and a palm finger part of a forefinger, the upper side of each fixing ring 111 is fixedly connected with a pipe frame 112, the pipe frame 112 comprises two semi-arc hoop bodies, the two hoop bodies are connected through bolts, the lower sides of the hoop bodies are fixedly connected with the fixing rings 111, the upper sides of the hoop bodies are fixedly provided with connecting plates, and bolt holes are formed in the connecting plates;
the pipeline part 12 comprises a movable pipe, each knuckle of the movable pipe corresponding to the index finger is divided into a fingertip pipe 121, a middle pipe 122 and a metacarpophalangeal pipe 123, and the fingertip pipe 121, the middle pipe 122 and the metacarpophalangeal pipe 123 are respectively sleeved with the pipe frame 112 of the fixing ring 111 corresponding to the fingertip part, the middle part and the metacarpophalangeal part;
one end of the fingertip tube 121 far away from the palm is connected with the seed receiving tube 2, one end of the metacarpophalangeal tube 123 facing the palm is connected with the air tube 3, and two ends of the middle tube 122 are respectively connected with the fingertip tube 121 and the metacarpophalangeal tube 123 through the connecting tubes 124; the connecting pipe 124 comprises a straight pipe body located in the middle, two ends of the straight pipe body are respectively provided with a ball block, the connecting pipe 124 is hollow, and the connecting pipe 124 is respectively in spherical hinge connection with the fingertip pipe 121, the middle pipe 122 and the palm and finger pipe 123 through the ball blocks.
The hoop body of tube carrier 112 is made of an elastic material.
The ball block and the joints of the finger tip tube 121, the middle tube 122 and the palm and finger tube 123 with the ball block are all made of elastic materials, such as PC soft glue. Select for use the elasticity material as connection structure, under the prerequisite of guaranteeing mobility, through appropriate interference fit, can make the junction from taking good damping force, can not lead to the inoculation pipe 2 to rock because of the slight action of finger unconsciousness.
The joints of the fingertip tube 121, the middle tube 122 and the palm finger tube 123 and the ball block are provided with annular elastic cushion rings.
Example 2
Referring to fig. 4, on the basis of the above embodiment, the air pump 4 includes a bag body 41 made of elastic material, a cushion block 42 is fixedly arranged on one side of the air pump 4, and a double-finger ring 43 corresponding to the ring finger and the little finger is fixedly arranged on the other side of the air pump 4;
the bag body 41 is provided with an air nozzle which is communicated with the air pipe 3.
The through hole is formed in the double-finger ring 43, and two convex arc-shaped surfaces are arranged on one side, away from the capsule body 41, of the through hole, so that the design is more in line with the structure of a human body, and the comfort level of operation is improved.
When the air pump is used, an operator holds the bag body 41 of the air pump 4 in the palm, the ring finger and the little finger are placed in the two-finger ring 43, the cushion block 42 is attached to the palm, and the suction force of the air pump 4 can be transmitted to the pipeline part 12 through the air pipe 3 by the air pump 4 after the ring finger and the little finger are opened, so that the inoculation pipe 2 is pumped, and the inoculation pipe 2 sucks tissue particles through the action. After the tissue pellet is aspirated, the seeding tube 2 is inserted into the culture flask 100. The inoculation tube 2 is driven by the movement of the forefinger to search for a proper inoculation position in the culture bottle, then the third finger and the little finger press the sac body 41, and the tissue particles are inoculated on the inner wall of the culture bottle through air pressure.
Because the using-way restriction of traditional inoculation straw, the user need grip the inoculation straw and just can conveniently absorb and place the tissue grain, so when the inoculation bottle in the face of, then be difficult to the operation, this scheme realizes through forefinger external member 1 that only controls with forefinger butt joint seed pipe 2 position, when using the blake bottle as culture container, the operation accords with human custom more.
Example 3
Referring to fig. 5 and 6, on the basis of the above embodiment, the seed tube 2 includes a main tube 21 communicated with the fingertip tube 121, and after the seed tube 2 is connected with the fingertip tube 121, the main tube 21 is coaxial with the fingertip tube 121;
an inclined tube 22 which inclines upwards is arranged at one end of the main tube 21 far away from the fingertip tube 121, a flat tube 23 is arranged at one end of the inclined tube 22 far away from the main tube 21, the axis of the flat tube 23 is parallel to the axis of the main tube 21, a front end tube 24 is arranged at one end of the flat tube 23 far away from the main tube 21, and the front end tube 24 bends downwards.
Considering that the bottle mouth of the culture bottle is generally small and has a certain inclination, the inoculation tube 2 can be more conveniently inserted into the culture bottle 100 for inoculation operation through the structure of the inoculation tube 2.
Example 4
Referring to fig. 7, on the basis of the above embodiment, the fingertip tube 121 is connected with the inoculation tube 2 through the rotary drum 5, the rotary drum 5 is a cylinder, one end of the rotary drum 5 is rotatably connected with the fingertip tube 121, and the other end is inserted into the end of the inoculation tube 2; the two sides of the cylinder body of the rotary cylinder 5 are symmetrically provided with connecting rings 51;
the device further comprises a drawing kit 6, wherein the drawing kit 6 is divided into a thumb kit and a middle finger kit which correspond to a thumb and a middle finger, the thumb kit and the middle finger kit respectively comprise two parallel lantern rings 61, the two lantern rings 61 are fixedly connected through a connecting rod 62 arranged on one side, and one end, facing a fingertip, of the connecting rod 62 is fixedly provided with a pull ring 63;
the two connecting rings 51 of the drum 5 are each connected to a pull ring 63 of the middle finger set of the thumb set by a pull cord 7.
The connecting rod 62 is an elastic rod body, the two lantern rings of the thumb suite respectively correspond to the palm part and the fingertip part of the thumb, and the two lantern rings of the middle finger suite respectively correspond to the fingertip part and the middle part of the middle finger
The pull rope 7 is made of a slightly elastic material. A damping pad is arranged between the rotary drum 5 and the fingertip tube 121. Because the thumb and the middle finger can not be kept completely still in the inoculation process, particularly when the capsule body 41 is pinched, the middle finger is inevitably dragged by the ring finger, and the pulling rope 7 with slight elasticity can be adopted to offset the pulling force caused by the misoperation of the finger.
When the inoculating device is used, the thumb kit and the middle finger kit are respectively worn, and in the inoculating process, when the index finger drives the inoculating tube 2 and does not obtain a proper position, the pull rope 7 can be pulled by the thumb or the middle finger to rotate the rotary drum 5, so that the inoculating tube 2 rotates at a certain angle, and the operation in the culture bottle is more convenient.
The pterygoid lamina 25 is set up to the tip bilateral symmetry of inoculation pipe 2, corresponds pterygoid lamina 25 in the rotary drum 5 and sets up the slot, and rotary drum 5 passes through slot and pterygoid lamina 25 and pegs graft with inoculation pipe 2.
The plate body of the wing plate 25 is provided with a concave hole, and a spring ball is arranged in the slot of the rotary drum 5 corresponding to the concave hole.
The flange 25 is combined with the concave hole and the spring bead, so that the inoculation tube 2 and the fingertip tube 121 can be quickly disassembled.
Limiting plates 125 are symmetrically and fixedly arranged on the upper side and the lower side of the finger tip part 121, and wire passing holes are formed in the limiting plates 125 corresponding to the pull ropes 7.
During the use, the stay cords that correspond to the connecting rings 51 on both sides are respectively wound to different limiting plates 125 from the upper and lower sides of the rotary drum 5 and pass through the wire passing holes to be connected with the pull ring 63, so that mutual interference between the two stay cords 7 during pulling can be avoided, and the rotation of the rotary drum 5 is influenced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (1)
1. The inoculation device for muscle cell culture is characterized by comprising a forefinger kit (1) sleeved on a forefinger of a user, wherein the forefinger kit (1) comprises a finger ring part (11) used for being connected with a finger, a pipeline part (12) is connected to the upper side of the finger ring part, one end, close to the fingertip, of the pipeline part (12) is connected with a inoculation tube (2), the other end of the pipeline part is connected with an air pump (4) through an air tube (3), and the inoculation tube (2), the pipeline part (12), the air tube (3) and the air pump (4) are communicated with each other;
the ring part (11) of the index finger kit (1) comprises three fixing rings (111) which respectively correspond to a finger tip part, a middle part and a palm finger part of an index finger, the upper side of each fixing ring (111) is fixedly connected with a pipe frame (112), the pipe frame (112) comprises two semicircular arc-shaped hoop bodies, and the two hoop bodies are connected through bolts;
the pipeline part (12) comprises a movable pipe, each knuckle of the movable pipe, corresponding to the forefinger, is divided into a fingertip pipe (121), a middle pipe (122) and a palm finger pipe (123), and the fingertip pipe (121), the middle pipe (122) and the palm finger pipe (123) are respectively sleeved with the pipe frame (112) of the fingertip part, the middle part and the palm finger part corresponding to the fixing rings (111);
one end, far away from the palm, of the fingertip tube (121) is connected with the inoculation tube (2), one end, facing the palm, of the metacarpophalangeal tube (123) is connected with the air tube (3), and two ends of the middle tube (122) are respectively connected with the fingertip tube (121) and the metacarpophalangeal tube (123) through connecting tubes (124); the connecting pipe (124) comprises a straight pipe body positioned in the middle, two ends of the straight pipe body are respectively provided with a spherical block, the connecting pipe (124) is hollow, and the connecting pipe (124) is respectively in spherical hinge connection with the fingertip pipe (121), the middle pipe (122) and the palm and finger pipe (123) through the spherical blocks;
the air pump (4) comprises a bag body (41) made of elastic materials, a cushion block (42) is fixedly arranged on one side of the air pump (4), and a double-finger ring (43) corresponding to the ring finger and the little finger is fixedly arranged on the other side of the air pump;
the bag body (41) is provided with an air nozzle which is communicated with the air pipe (3);
the inoculation tube (2) comprises a main tube (21) communicated with the fingertip tube (121), and after the inoculation tube (2) is connected with the fingertip tube (121), the main tube (21) is coaxial with the fingertip tube (121);
the one end that is kept away from finger tip pipe (121) in being responsible for (21) sets up tube chute (22) of tilt up, and the one end that is kept away from in tube chute (22) is set up plain tube (23), the axis of plain tube (23) with the axis of being responsible for (21) is parallel, the one end that is kept away from in plain tube (23) is set up front end pipe (24), front end pipe (24) are buckled downwards.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011480270.9A CN112592812B (en) | 2020-12-15 | 2020-12-15 | Inoculation device for muscle cell culture |
| CN202210775251.1A CN115109688A (en) | 2020-12-15 | 2020-12-15 | Inoculation instrument for muscle cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011480270.9A CN112592812B (en) | 2020-12-15 | 2020-12-15 | Inoculation device for muscle cell culture |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210775251.1A Division CN115109688A (en) | 2020-12-15 | 2020-12-15 | Inoculation instrument for muscle cell culture |
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| Publication Number | Publication Date |
|---|---|
| CN112592812A CN112592812A (en) | 2021-04-02 |
| CN112592812B true CN112592812B (en) | 2022-08-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011480270.9A Active CN112592812B (en) | 2020-12-15 | 2020-12-15 | Inoculation device for muscle cell culture |
| CN202210775251.1A Pending CN115109688A (en) | 2020-12-15 | 2020-12-15 | Inoculation instrument for muscle cell culture |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210775251.1A Pending CN115109688A (en) | 2020-12-15 | 2020-12-15 | Inoculation instrument for muscle cell culture |
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Also Published As
| Publication number | Publication date |
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| CN115109688A (en) | 2022-09-27 |
| CN112592812A (en) | 2021-04-02 |
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