LU500075B1 - An inoculation device for muscle cell culture - Google Patents

Abstract

The invention discloses an inoculation device for muscle cell culture, which relates to the field of cell culture equipment. The technical scheme is as follows: the inoculation device comprises an index finger kit sleeved on the index finger of a user, wherein the index finger kit comprises a finger ring part for connecting with fingers. The upper side of the finger ring part is connected with a pipeline part. One end of the pipeline part close to fingertips is connected with an inoculation pipe. The other end is connected with an air pump through an air pipe.The inoculation pipe, the pipeline part, the air pipe and the air pump are internally communicated. The invention has the beneficial effects that: by changing the traditional inoculation tool form, the scheme adopts a structure more in line with the inoculation operation of the culture bottle.

Description

DESCRIPTION An inoculation device for muscle cell culture

TECHNICAL FIELD The invention relates to the field of cell culture equipment, in particular to an inoculation device for muscle cell culture.

BACKGROUND In the process of muscle cell culture, muscle tissue should be inoculated into culture bottle or Petri dish after being processed into tissue granules. The inoculation tools are usually ophthalmic tweezers, pipes or inoculation rings. For inoculation containers, Petri dishes and culture bottles are commonly adopted. Because the lid of the Petri dish can be completely removed, it is convenient to operate during inoculation. During inoculation, the tissue particles are dispersed in the Petri dish, which needs to wait for a certain time for the tissue to adhere to the wall. After the adherence is completed, the Petri dish needs to be opened again to add nutrient solution, which may cause the tissue particles to be infected by bacteria in the process. Culture bottle can be used to solve this problem. Due to its structure, inoculation and nutrient solution injection can be completed at the same time. After the tissue adhered to the wall, the culture bottle can be rotated to make the nutrient solution contact with the tissue. In this process, there is no need to open the bottle. However, due to the limitation of the structure of the culture bottle, when the traditional inoculation tools are used to inoculate with the culture bottle, the inoculation operation is very inconvenient because the bottle mouth is relatively small.

SUMMARY In view of the above technical problems, the present invention provides an inoculation device for muscle cell culture.

According to the technical scheme, the index finger kit comprises a finger ring part for connecting with fingers.

The upper side of the finger ring part is connected with a pipeline part.

One end of the pipeline part close to fingertips is connected with an inoculation pipe.

The other end is connected with an air pump through an air pipe.

The inoculation pipe, the pipeline part, the air pipe and the air pump are internally communicated.

Preferably, the finger ring part of the index finger kit comprises three fixing rings, which respectively correspond to the fingertip part, the middle part and the metacarpal part of the index finger, and the upper side of each fixing ring is fixedly connected with a pipe rack, which comprises two semi-circular hoop bodies which are connected by bolts.

The lower side of the hoop bodies are fixedly connected with the fixing ring, and the upper side of the hoop bodies is fixedly provided with a connecting plate which is provided with bolt holes.

The pipeline part comprises a movable pipe, which is divided into a fingertip pipe, an intermediate pipe and a metacarpal pipe corresponding to each knuckle of the index finger.

The fingertip pipe, the intermediate pipe and the metacarpal pipe are respectively sleeved with the pipe racks of the fixing ring corresponding to the fingertip part, the middle part and the metacarpal part.

The end of the fingertip pipe far from the palm is connected with the inoculation pipe, the end of the metacarpal pipe facing the palm is connected with the air pipe, and the two ends of the intermediate pipe are respectively connected with the fingertip pipe and the metacarpal pipe through connecting pipes.

The connecting pipe comprises a straight pipe body located in the middle, two ends of the straight pipe body are respectively provided with ball blocks, the inside of the connecting pipe is hollow, and the connecting pipe is respectively connected with the fingertip pipe, the intermediate pipe and the metacarpal pipe by ball blocks in a ball hinge way.

Preferably, the hoop bodies of the pipe rack is made of elastic material.

Preferably, the ball block, the fingertip pipe, the intermediate pipe and the joints between the metacarpal pipe and the ball block are all made of elastic materials, such as PC soft material.

The elastic material is selected as the connecting structure, and under the premise of ensuring mobility, the joint can bring good damping force through proper interference fit, and the inoculation pipe will not shake because of the slight involuntary movement of fingers.

Preferably, annular elastic cushion rings are arranged at the joints of the fingertip pipe, the intermediate pipe and the metacarpal pipe and the ball block.

Preferably, the air pump comprises a bag body made of elastic material, a cushion block is fixedly arranged on one side of the air pump, and a double finger ring corresponding to the ring finger and the little finger is fixedly arranged on the other side.

An air nozzle is arranged on the bag body, and the air nozzle is communicated with the air pipe.

Preferably, a through hole is arranged in the double finger ring, and two convex arc surfaces are arranged on one side of the through hole far away from the bag body, so that the design is more in line with the structure of the human body and improves the operating comfort.

When in use, the operator holds the bag body of the air pump in the palm, the ring finger and the little finger are put into the double finger ring, and the cushion block is attached to the palm.

By opening the ring finger and the little finger, the air pump can pump air.

Because the air pump pumps air, the suction force is transmitted to the pipeline through the air pipe, so that the inoculation pipe can pump air.

Through this action, the inoculation pipe can absorb tissue particles.

After sucking the tissue granules, extend the inoculation pipe into the culture bottle.

Through the movement of index finger, the inoculation pipe is driven to find a suitable inoculation position in the culture bottle, then the ring finger and little finger press the bag body, and the tissue particles are inoculated on the inner wall of the culture bottle by air pressure.

Because the use of traditional inoculation pipes is limited, the user needs to hold the inoculation pipes to suck and place the tissue particles conveniently, so it is difficult to operate when facing the inoculation bottle.

In this scheme, the position of the inoculation pipe is controlled only by index finger kit, and the operation is more in line with human habits when using the culture bottle as the culture container.

Preferably, the inoculation pipe comprises a main pipe communicating with the fingertip pipe, and after the inoculation pipe is connected with the fingertip pipe, the main pipe is coaxial with the fingertip pipe.

The end of the main pipe away from the fingertip pipe is provided with an inclined pipe which 1s inclined upwards, and the end of the inclined pipe away from the main pipe 1s provided with a flat pipe whose axis is parallel to that of the main pipe.

The end of the flat pipe far away from the main pipe 1s provided with a front end pipe, and the front end pipe is bent downward.

Considering that the bottle mouth of the culture bottle is generally small and has a certain inclination, it is more convenient to probe the inoculation pipe into the culture bottle for inoculation operation through the structure of the inoculation pipe.

Preferably, the fingertip pipe is connected with the inoculation pipe through a rotating drum which is a cylinder, and one end of the rotating drum is rotatably connected with the fingertip pipe and the other end is inserted with the end of the inoculation pipe.

Connecting rings are symmetrically arranged on both sides of the cylinder body of the rotating drum.

The device also comprises a pulling kit which is divided into a thumb kit and a middle finger kit corresponding to the thumb and the middle finger, wherein both the thumb kit and the middle finger kit comprise two parallel collars, and the two collars are fixedly connected through a connecting rod arranged at one side, and a pull ring is fixedly arranged at one end of the connecting rod facing the fingertip.

Two connecting rings of the rotating drum are respectively connected with the pull ring of the middle finger kit of the thumb kit through a drawstring.

Preferably, the connecting rod is an elastic rod body, one collar of the thumb kit respectively corresponds to the metacarpal part and fingertip part of the thumb, and two collars of the middle finger kit respectively correspond to the fingertip part and middle part of the middle finger.

Preferably, the drawstring 1s made of a slightly elastic material.

When in use, wear the thumb kit and the middle finger kit respectively.

In the inoculation process, when the index finger drives the inoculation pipe and does not get the proper position, the drawstring can be pulled by the thumb or middle finger to make the rotating drum rotate, thus making the inoculation pipe rotate at a certain angle, so that it is more convenient to operate in the culture bottle.

Preferably, wing plates are symmetrically arranged on both sides of the end of the inoculation pipe, slots are arranged in the rotating drum corresponding to the wing plates, and the rotating drum is inserted with the inoculation pipe through the slots and the wing plates.

Preferably, a concave hole is arranged on the plate body of the wing plate, and a spring ball is arranged in the slot of the rotating drum corresponding to the concave hole.

The wing plate is combined with the concave hole and the spring ball, so that the inoculation pipe and the fingertip pipe can be quickly disassembled.

Preferably, limiting plates are symmetrically and fixedly arranged on the upper and lower sides of the fingertip part, and wire passing holes are arranged on the limiting plates corresponding to the drawstring.

When in use, the drawstrings corresponding to the connecting rings on both sides are respectively wound from the upper and lower sides of the rotating drum to different limiting plates and connected with the pull ring through the wire passing holes, so that mutual interference between the two drawstrings during pulling can be avoided, thereby affecting the rotation of the rotating drum. The technical scheme provided by the embodiment of the invention has the beneficial effects that: by changing the traditional inoculation tool form, the scheme adopts a structure more in line with the inoculation operation of the culture bottle, and by associating the inoculation tool with each finger, it is more convenient to extend the inoculation pipe into the culture bottle for operation.

DESCRIPTION OF THE FIGURES Fig. 1 is a schematic diagram of the overall structure of the embodiment of the present invention. Fig. 2 is a partial enlarged view of A in Fig. 1. Fig. 3 is a schematic structural diagram of the connecting pipe according to the embodiment of the present invention. Fig. 4 is a schematic structural diagram of the air pump according to the embodiment of the present invention. Fig. 5 is a schematic structural diagram of the inoculation pipe according to the embodiment of the present invention. Fig. 6 is a front view of the inoculation pipe structure according to the embodiment of the present invention. Fig. 7 is a partial enlarged view of A in Fig. 2. Wherein, the reference numbers are: 100, culture bottle. 1, index finger kit. 11, finger ring part. 111, fixing ring. 112, pipe rack. 12, pipeline part. 121, fingertip pipe. 122, intermediate pipe. 123, metacarpal pipe. 124, connecting pipe. 125, limiting plate. 2,

inoculation pipe. 21, horizontal pipe. 22, inclined pipe. 23, flat pipe. 24, front end pipe. 3, air pipe. 4, air pump. 41, bag body. 42, cushion block. 43, double finger ring. 5, rotating drum. 51, connecting ring. 6, pulling kit. 61, collar. 62, connecting rod. 63, pull ring. 7, drawstring.

DESCRIPTION OF THE INVENTION In order to make the object, technical scheme and advantages of the present invention clearer, the present invention will be further described in detail with reference to the figures and embodiments. Of course, the specific embodiments described here are only used to explain the present invention, and are not used to limit the present invention. It should be noted that the embodiments in the invention and the features in the embodiments can be combined with each other without conflict. In the description of the invention, it should be understood that the orientation or position relationship indicated by the terms "center", "longitudinal", "transversal", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" and "inner" and others is based on the orientation or position relationship shown in the figure, which is only for the convenience of describing the invention creation and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation and be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation of the invention creation. In addition, the terms "first", "second" and the like are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined by "first", "second",

etc, may explicitly or implicitly include one or more of the features.

In the description of the invention, unless otherwise specified, "multiple" means two or more.

In the description of the invention, it should be noted that unless otherwise specified and limited, the terms "installation", "connection", "communication" and "setting" should be understood in a broad sense, for example, they can be fixed connection, detachable connection or integrated connection.

It can be connected mechanically or electrically.

It can be directly connected, indirectly connected through an intermediate medium, or communicated inside two elements.

For ordinary technicians in the field, the specific meanings of the above terms in the invention can be understood through specific situations.

Embodiment 1 With reference to fig. 1 to fig. 3, the present invention provides an inoculation device for muscle cell culture, which comprises an index finger kit (1) sleeved on a user's index finger, the index finger kit (1) comprises a finger ring part (11) for connecting with the finger, and the upper side of the finger ring part is connected with a pipeline part (12). One end of the pipeline part close to fingertips is connected with an inoculation pipe (2). The other end is connected with an air pump (4) through an air pipe (3). The inoculation pipe (2), the pipeline part (12), the air pipe (3) and the air pump (4) are internally communicated.

The finger ring part (11) of the index finger kit(1) comprises three fixing rings (111), which respectively correspond to the fingertip part, the middle part and the metacarpal part of the index finger, and the upper side of each fixing ring (111) is fixedly connected with a pipe rack (112), which comprises two semi-circular hoop bodies which are connected by bolts.

The lower side of the hoop bodies is fixedly connected with the fixing ring (111), and the upper side of the hoop bodies is fixedly provided with a connecting plate with bolt holes.

The pipeline part (12) comprises a movable pipe, which is divided into a fingertip pipe (121), an intermediate pipe (122) and a metacarpal pipe (123) corresponding to each knuckle of the index finger.

The fingertip pipe (121), the intermediate pipe (122) and the metacarpal pipe (123) are respectively sleeved with the pipe racks (112) of the fixing ring (111) corresponding to the fingertip part, the middle part and the metacarpal part.

The end of the fingertip pipe (121) far from the palm is connected with the inoculation pipe (2), the end of the metacarpal pipe (123) facing the palm is connected with the air pipe (3), and the two ends of the intermediate pipe (122) are respectively connected with the fingertip pipe (121) and the metacarpal pipe (123) through connecting pipes (124). The connecting pipe (124) comprises a straight pipe body located in the middle, two ends of the straight pipe body are respectively provided with ball blocks, the inside of the connecting pipe (124) is hollow, and the connecting pipe (124) is respectively connected with the fingertip pipe (121), the intermediate pipe (122) and the metacarpal pipe (123) by ball blocks in a ball hinge way.

The hoop bodies of the pipe rack 112 is made of elastic material.

The ball block, the fingertip pipe (121), the intermediate pipe (122) and the joints between the metacarpal pipe (123) and the ball block are all made of elastic materials, such as PC soft material.

The elastic material is selected as the connecting structure, and under the premise of ensuring mobility, the joint can bring good damping force through proper interference fit, and the inoculation pipe (2) will not shake because of the slight involuntary movement of fingers.

Circular elastic cushion rings are arranged at the joints of the fingertip pipe (121), the intermediate pipe (122), the metacarpal pipe (123) and the ball block.

Embodiment 2 Referring to fig. 4, on the basis of the above embodiment, the air pump (4) comprises a bag body (41) made of elastic material, a cushion block (42) is fixedly arranged on one side of the air pump (4), and a double finger ring (43) corresponding to the ring finger and the little finger is fixedly arranged on the other side.

An air nozzle is arranged on the bag body (41) and is communicated with the air pipe (3). A through hole is formed in the double finger ring (43), and the side of the through hole far away from the bag body (41) is provided with two convex arc surfaces, which is more in line with the structure of the human body and improves the operating comfort.

When in use, the operator holds the bag body (41) of the air pump (4) in the palm, the ring finger and the little finger are put into the double finger ring (43), and the cushion block (42) is attached to the palm.

By opening the ring finger and the little finger, the air pump (4) can pump air.

Because the air pump (4) pumps air, the suction force is transmitted to the pipeline (12) through the air pipe (3), so that the inoculation pipe (2) can pump air.

Through this action, the inoculation pipe (2) can absorb tissue particles.

After sucking the tissue granules, extend the inoculation pipe (2) into the culture bottle (100). Through the movement of index finger, the inoculation pipe (2) is driven to find a suitable inoculation position in the culture bottle, then the ring finger and little finger press the bag body (41), and the tissue particles are inoculated on the inner wall of the culture bottle by air pressure.

Because the use of traditional inoculation pipes 1s limited, the user needs to hold the inoculation pipes to suck and place the tissue particles conveniently, so it is difficult to operate when facing the inoculation bottle.

In this scheme, the position of the inoculation pipe (2) is controlled by index finger kit (1), and the operation is more in line with human habits when using the culture bottle as a culture container.

Embodiment 3 Referring to fig. 5 and fig. 6, on the basis of the above embodiments, the inoculation pipe (2) comprises a main pipe (21) communicating with the fingertip pipe (121), and after the inoculation pipe (2) is connected with the fingertip pipe (121), the main pipe (21) is coaxial with the fingertip pipe (121). The end of the main pipe (21) away from the fingertip pipe (121) is provided with an inclined pipe (22) which is inclined upwards, and the end of the inclined pipe (22) away from the main pipe (21) 1s provided with a flat pipe (23) whose axis is parallel to that of the main pipe (21). The end of the flat pipe (23) far away from the main pipe (21) is provided with a front end pipe (24), and the front end pipe (24) is bent downward.

Considering that the bottle mouth of the culture bottle is generally small and has a certain inclination, it is more convenient to probe the inoculation pipe (2) into the culture bottle (100) for inoculation operation through the above-mentioned structure of the inoculation pipe (2). Embodiment 4

Referring to fig. 7, on the basis of the above embodiment, the fingertip pipe (121) is connected with the inoculation pipe (2) through a rotating drum (5) which is a cylinder, and one end of the rotating drum (5) is rotatably connected with the fingertip pipe (121) and the other end is inserted with the end of the inoculation pipe (2). Connecting rings (51) are symmetrically arranged on both sides of the cylinder body of the rotating drum (5). The device also comprises a pulling kit (6) which is divided into a thumb kit and a middle finger kit corresponding to the thumb and the middle finger, wherein both the thumb kit and the middle finger kit comprise two parallel collars (61), and the two collars (61) are fixedly connected through a connecting rod (62) arranged at one side, and a pull ring (63) is fixedly arranged at one end of the connecting rod (62) facing the fingertip.

Two connecting rings (51) of the rotating drum (5) are respectively connected with the pull ring (63) of the middle finger kit of the thumb kit through a drawstring (7). The connecting rod (62) 1s an elastic rod body, one collar of the thumb set corresponds to the metacarpal part and the fingertip part of the thumb respectively, and two collars of the middle finger kit correspond to the fingertip part and the middle part of the middle finger respectively.

The drawstring (7) is made of slightly elastic material.

A damping pad is provided between the rotating drum (5) and the fingertip pipe (121). Because the thumb and middle finger are not completely stationary during the inoculation process, especially when the bag body (41) is pinched, the middle finger is inevitably pulled by the ring finger, and the pulling force caused by finger misoperation can be offset by using the slightly elastic drawstring (7).

When in use, wear the thumb kit and the middle finger kit respectively.

When the index finger drives the inoculation pipe (2) and does not get a proper position in the inoculation process, the drawstring (7) can be pulled by the thumb or the middle finger to rotate the rotating drum (5), so that the inoculation pipe (2) can rotate at a certain angle, making it more convenient to operate in the culture bottle.

Wing plates (25) are symmetrically arranged on both sides of the end of the inoculation pipe (2), slots are arranged in the rotating drum (5) corresponding to the wing plates (25), and the rotating drum (5) is inserted with the inoculation pipe (2) through the slots and the wing plates (25). A concave hole is arranged on the plate body of the wing plate (25), and a spring ball is arranged in the slot of the rotating drum (5) corresponding to the concave hole.

By combining the wing plate (25) with the concave hole and the spring ball, the inoculation pipe (2) and the fingertip pipe (121) can be quickly detached.

Limiting plates (125) are symmetrically and fixedly arranged on the upper and lower sides of the fingertip part (121), and wire passing holes are arranged on the limiting plates (125) corresponding to the drawstring (7). In use, the drawstrings corresponding to the connecting rings (51) on both sides are respectively wound from the upper and lower sides of the rotating drum (5) to different limiting plates (125) and connected with the pull ring (63) through the thread passing holes, so that the interference between the two drawstrings (7) during pulling can be avoided, thus affecting the rotation of the rotating drum (5). The above content is only preferred embodiments of the present invention, and is not intended to limit the present invention.

Any modifications, equivalent substitutions,

improvements, etc., made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.

Claims (9)

1. An inoculation device for muscle cell culture, characterized by comprising an index finger kit (1) sleeved on the index finger of a user, wherein the index finger kit (1) comprises a finger ring part (11) for connecting with the finger, and the upper side of the finger ring part is connected with a pipeline part (12); one end of the pipeline part close to fingertips 1s connected with an inoculation pipe (2); the other end is connected with an air pump (4) through an air pipe (3); the inoculation pipe (2), the pipeline part (12), the air pipe (3) and the air pump (4) are internally communicated.

2. The inoculation device for muscle cell culture according to claim 1, characterized in that the finger ring part (11) of the index finger kit (1) comprises three fixing rings (111), which respectively correspond to the fingertip part, the middle part and the metacarpal part of the index finger, and the upper side of each fixing ring (111) is fixedly connected with a pipe rack (112), which comprises two semi-circular hoop bodies which are connected by bolts; the pipeline part (12) comprises a movable pipe, which is divided into a fingertip pipe (121), an intermediate pipe (122) and a metacarpal pipe (123) corresponding to each knuckle of the index finger; the fingertip pipe (121), the intermediate pipe (122) and the metacarpal pipe (123) are respectively sleeved with the pipe racks (112) of the fixing ring (111) corresponding to the fingertip part, the middle part and the metacarpal part; the end of the fingertip pipe (121) far from the palm is connected with the inoculation pipe (2), the end of the metacarpal pipe (123) facing the palm is connected with the air pipe (3), and the two ends of the intermediate pipe (122) are respectively connected with the fingertip pipe (121) and the metacarpal pipe (123) through connecting pipes (124); the connecting pipe (124) comprises a straight pipe body located in the middle, two ends of the straight pipe body are respectively provided with ball blocks, the inside of the connecting pipe (124) 1s hollow, and the connecting pipe (124) 1s respectively connected with the fingertip pipe (121), the intermediate pipe (122) and the metacarpal pipe (123) by ball blocks in a ball hinge way.

3. The inoculation device for muscle cell culture according to claim 1, characterized in that the air pump (4) comprises a bag body (41) made of elastic material, a cushion block (42) is fixedly arranged on one side of the air pump (4), and a double finger ring (43) corresponding to the ring finger and the little finger is fixedly arranged on the other side; an air nozzle is arranged on the bag body (41) and is communicated with the air pipe (3).

4. The inoculation device for muscle cell culture according to claim 1, characterized in that the inoculation pipe (2) comprises a main pipe (21) communicating with the fingertip pipe (121), and after the inoculation pipe (2) is connected with the fingertip pipe (121), the main pipe (21) is coaxial with the fingertip pipe (121); the end of the main pipe (21) away from the fingertip pipe (121) is provided with an inclined pipe (22) which is inclined upwards, and the end of the inclined pipe (22) away from the main pipe (21) 1s provided with a flat pipe (23) whose axis is parallel to that of the main pipe (21); the end of the flat pipe (23) far away from the main pipe (21) is provided with a front end pipe (24), and the front end pipe (24) is bent downward.

5. The inoculation device for muscle cell culture according to claim 2, characterized in that the fingertip pipe (121) is connected with the inoculation pipe (2) through a rotating drum (5) which is a cylinder, and one end of the rotating drum (5) is rotatably connected with the fingertip pipe (121) and the other end is inserted with the end of the inoculation pipe (2); connecting rings (51) are symmetrically arranged on both sides of the cylinder body of the rotating drum (5); the device also comprises a pulling kit (6) which is divided into a thumb kit and a middle finger kit corresponding to the thumb and the middle finger, wherein both the thumb kit and the middle finger kit comprise two parallel collars (61), and the two collars (61) are fixedly connected through a connecting rod (62) arranged at one side, and a pull ring (63) is fixedly arranged at one end of the connecting rod (62) facing the fingertip; two connecting rings (51) of the rotating drum (5) are respectively connected with the pull ring (63) of the middle finger kit of the thumb kit through a drawstring (7).

6. The inoculation device for muscle cell culture according to claim 5, characterized in that the drawstring (7) is made of elastic material.

7. The inoculation device for muscle cell culture according to claim 5, characterized in that wing plates (25) are symmetrically arranged on both sides of the end of the inoculation pipe (2), slots are arranged in the rotating drum (5) corresponding to the wing plates (25), and the rotating drum (5) is inserted with the inoculation pipe (2) through the slots and the wing plates (25).

8. The inoculation device for muscle cell culture according to claim 7, characterized in that a concave hole is arranged on the plate body of the wing plate (25), and a spring ball is arranged in the slot of the rotating drum (5) corresponding to the concave hole.

9. The inoculation device for muscle cell culture according to claim 5, characterized in that limiting plates (125) are symmetrically and fixedly arranged on the upper and lower sides of the fingertip part (121), and wire passing holes are arranged on the limiting plates (125) corresponding to the drawstring (7).