CN207418767U - A kind of suspension cell culture device being synchronously administered - Google Patents
A kind of suspension cell culture device being synchronously administered Download PDFInfo
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- CN207418767U CN207418767U CN201721483610.7U CN201721483610U CN207418767U CN 207418767 U CN207418767 U CN 207418767U CN 201721483610 U CN201721483610 U CN 201721483610U CN 207418767 U CN207418767 U CN 207418767U
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- bottle
- suspension cell
- cell culture
- taper
- culture device
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Abstract
The utility model discloses a kind of suspension cell culture device being synchronously administered, including at least two taper blake bottles, delivery device, more siphunculus and blender；Each taper blake bottle includes bottle and bottle cap, and bottle inner wall is equipped with hydrophobic layer, and bottle cap is equipped with a hole；The outlet of delivery device is connected by connecting conduit with an entrance of more siphunculus, other outlets of more siphunculus pass sequentially through connecting conduit, delivery catheter, taper blake bottle bottle cap on hole connected with taper blake bottle；Taper blake bottle is placed on blender, and rotor, turn on agitator are placed in conical flask, and rotor rotates in taper blake bottle.The utility model can carry out normal sterile culture to a variety of suspension cells, can synchronously for a variety of suspension cells give same drug intervene processing and ensure it is at the uniform velocity controllable in administration process, help to record at any time a variety of suspension cell cultures, administration the specific time and can timing pharmaceutical intervention is carried out to each suspension cell and each suspension cell growth is good.
Description
Technical field
The utility model is related to biological technical fields, and in particular to a kind of suspension cell culture device being synchronously administered.
Background technology
Cell culture (cell culture) is one of important technology in preclinical medicine, applies relatively broad, mesh
Preceding its has penetrated into the numerous areas such as cell biology, pharmacology, biochemistry, toxicology, clinical examination, pharmacodynamics.With
The development of science and technology, since a nearly century, the mankind gradually develop cell culture technology, are studied, are improved, and obtain
Certain progress has gradually formed two dimension, (Zhao Diandian, Hou Lingling, Zhang Jingsi wait three-dimensional cells to Three-dimensional cell culture technology
The development of culture technique and its application [J] the China cell biology journal in stem cell and tumour cell, 2015,37
(8)：1140–1150).
It is well known that the microenvironment of cell culture technology main analog internal body, promote cell can be with routine growth, numerous
It grows, pass on, freeze, do homework to observe the basic structure of cell and function, can carry out medicine to cell on this basis
Intervention situation (Hou Xueqin, Lin little Hua, Li Wei animal of the drug to cell is observed in object intervention in vitro under controllable primary condition
The present situation of cell culture technology research and thinking [J] Liaoning University of TCM journal, 2010,12 (11)：37-39.).
Typically, cell culture technology mainly includes suspended cell culture technic, attached cell culture technique, wherein before
The application range of person is than broader, such as (history can reflect point of selenium inducing leukemia cell autophagies and apoptosis various leukaemia
Handset system [D] Beijing Union Medical College, 2014.), normal lymphocytes (account for modern Shun, Wu Lizhuan, Li Lili wait metallothioneins
In vain to influence [J] Acta Pratacultures of in vitro culture milk cow lymphocyte under heat stress, 2014,23 (3)：215-223.) etc. adopt
It is cultivated with suspended cell culture technic.
Existing suspended cell culture technic is tended not to the dry of the synchronizing of suspension cell of multiple and different types
Pre- and suspension cell be easy to cause pollution in incubation, this just synchronization intervention for a variety of suspension cells and its conventional training
It supports and brings huge challenge.
For the shortcoming of existing suspended cell culture technic, the utility model patent is developed and a can be synchronized to
The suspension cell culture device of medicine, such suspension cell culture device help to carry out normal sterile culture to a variety of suspension cells
And a variety of suspension cell growths are in good condition, help synchronous to give same drug for a variety of suspension cells and carry out at intervention
Reason and ensure it is at the uniform velocity controllable in administration process, help to record at any time a variety of suspension cell cultures, administration the specific time and can
With timing to a variety of suspension cells carry out pharmaceutical intervention, most ensure at last active and effectively to a variety of suspension cells at the uniform velocity controllably to
The synchronousness of medicine and a variety of suspension cell growths are in good condition.
The content of the invention
To solve above-mentioned the deficiencies in the prior art, the utility model provides the suspension cell culture device being synchronously administered.
The technical solution of the utility model is as follows：
A kind of suspension cell culture device being synchronously administered, including at least two taper blake bottles, delivery device, more siphunculus and
Blender；
Each taper blake bottle includes bottle and bottle cap, and the bottle inner wall is equipped with hydrophobic layer, is set on the bottle cap
There is a hole；
The outlet of the delivery device is connected by connecting conduit with an entrance of more siphunculus, other outlets of more siphunculus
Pass sequentially through connecting conduit, delivery catheter, taper blake bottle bottle cap on hole connected with taper blake bottle；
The taper blake bottle is placed on blender, and rotor, turn on agitator are placed in conical flask, and rotor is trained in taper
It supports in bottle and rotates.
Preferably, an air-permeative filter film, the air-permeative filter film institute are equipped between the bottle and bottle cap of the taper blake bottle
Corresponding part bottle cap is engraved structure.
Preferably, the hole is the latex aperture that can be stretched.
Preferably, the junction of the outlet of the junction of connecting conduit and more siphunculus, connecting conduit and delivery device, be connected and lead
The junction of pipe and delivery catheter is sealed by sealing fastening strip.
Preferably, the end of the delivery catheter is an administration needle, and the needle handle of the administration needle is arranged on outside bottle cap.
Preferably, the delivery device is piston injector, including delivery device outlet, to cylindrantherae and piston；
The piston includes the circular plug, intermediate circular pin and the oval handle of end of front end, circle plug and circular pin
In to cylindrantherae.
It is further preferred that the circular pin one side is equipped with wavy rack, it is described to be equipped with two protrusions to cartridge end,
In protrusion be equipped with one and fix short slab, the fixing axle of a manual gear is fixed on the fixation short slab, and the gear and circle
Wavy rack on shape bar tightly engages, rotate gear, will promote circular pin movement.
Preferably, a thermal insulation film is equipped between the mini-sized blender and taper blake bottle.
Preferably, the mini-sized blender is equipped with timer, which is equipped with timer display screen, timer is opened
Close button, timer forwarding button, timer backspace button and Timer modes button.
Preferably, the hydrophobic layer is silicone hydroxyl layer.
Preferably, the volume of the taper blake bottle is 150mL, and taper blake bottle outer surface is equipped with scale value.
Preferably, the rotor is cylindrical rotor.
Preferably, the thickness of the air-permeative filter film is 0.22 μm.
The utility model tool has the advantage that：
(1) the taper blake bottle volume of the utility model is 150mL, conventional appropriate beneficial to being carried out to a variety of suspension cells
Culture；There is taper blake bottle scale value in taper blake bottle outer surface, beneficial to culture solution in observation suspending cell culture bottle at any time
Volume.
(2) the silicone hydroxyl layer in taper blake bottle helps to reduce blake bottle inner surface free energy, and suspension cell is avoided to go out
Existing sticking phenomenon increases the quantity of suspension cell in the medium, avoids suspension cell because anoxic and the phenomena of mortality.
(3) mini-sized blender (50 turns/min) and cylindrical rotor contribute to a variety of suspension cells to be in suspension always
State avoids a variety of suspension cells because adherent and anoxic.
(4) thermal insulation film contributes in mini-sized blender operation process to avoid the thermal energy of generation passing to suspension carefully
Born of the same parents avoid affecting the growth of a variety of suspension cells with this.
(5) it is taper culture bottle cap above taper blake bottle, between taper blake bottle bottle and taper culture bottle cap
Have the air-permeative filter film of one layer of 0.22 μ m-thick, fully obtain the gas of needs beneficial to a variety of suspension cells, and can to gas into
Row filtering, avoids a variety of suspension cells from polluting.
(6) there is the latex aperture that can be stretched in taper culture bottle cap center, and latex aperture supplies the administration needle of delivery catheter
Head insertion, contacts with the external world if the latex aperture of elasticity helps to isolate a variety of suspension cells, avoids a variety of suspension cells micro-
Biological pollution.
(7) administration needle head side is administration needle handle, and administration needle handle is arranged on outside bottle cap, and administration needle head is followed by delivery catheter,
Delivery catheter is connected to connecting conduit by sealing fastening strip, and connecting conduit is connected to the port of export of more siphunculus, and connects
Place has sealing fastening strip to be sealed, and the arrival end of more siphunculus is connected to connecting conduit, and junction has sealing fastening strip to carry out
Sealing, the arrival end of more siphunculus connect the port of export of delivery device by connecting conduit, and junction has sealing fastening strip to be sealed,
These designs are beneficial to 1 entrance, 3 bases for exporting to reach synchronization administration for sealing delivery system and can be by more siphunculus
This process.
(8) delivery device outer surface is delivery device scale value, beneficial to being monitored at any time to dosage and record administration at any time
Amount number.
(9) inside delivery device it is circular plug, circle plug is followed by circular pin, is oval handle behind circular pin, circular
There is wavy rack in bar one side, and wavy rack is closely engaged with manual gear, and the center of manual gear is fixing axle, fixing axle
Positioned at fixed short slab one end, the fixed short slab other end, which is connected to, gives cylindrantherae afterbody one side, these designs are beneficial to evenly, controllably
Same pharmaceutical intervention is carried out to a variety of suspension cells, avoids too fast on the administration of a variety of suspension cells bringing unnecessary influence.
(10) there is timer on mini-sized blender surface, and there are timer display screen, timer switch button in timer surface, determine
When device forwarding button, timer backspace button, Timer modes button, these designs be beneficial to synchronize a variety of suspension cells to
Medicine, is timed control and time, pharmaceutically-active time, time etc. of a variety of suspension cell cultures that record is administered every time.
Description of the drawings
Fig. 1 is the overall structure diagram of the utility model.
Fig. 2 is the Bottle cap structure schematic diagram of the taper blake bottle of the utility model.
Fig. 3 is the timer operation panel schematic diagram of the utility model.
Reference sign：0- mini-sized blenders；1st, 8,15- taper blake bottles；2nd, 9,16- silicone hydroxyl layers；44、47、50-
Taper blake bottle scale value；3rd, 10,17- bottle caps；58th, 59,60- latex apertures；4th, 11,18- air-permeative filter films；7th, 14,21- gives
Medicine conduit；5th, 12,19- administration needle heads；6th, 13,20- administrations needle handle；22nd, 23,24,29,28,31,32,34- sealing fastening strips；
25th, 26,27,33- connecting conduits；30- four-way pipes；35- delivery devices export；36- delivery devices；The circular plugs of 37-；38- circular pins；
39- fixes short slab；40- fixing axles；41 manual gears；The wavy racks of 42-；43- ellipse handles；45- timers；53- timings
Device display screen；
54- timer switch buttons；55- timer forwarding buttons；56- timer backspace buttons；57- Timer modes are pressed
Button；46th, 48,51- cylindrical rotors；49- thermal insulation films；52- delivery device scale values.
Specific embodiment
In conjunction with implementation and the construction, shape and the further details of explanation of feature of Fig. 1-3 pairs of the utility model.It should manage
Solution, the present embodiment are merely to illustrate the utility model rather than limit the scope of protection of the utility model.
It is as shown in Figure 1 the suspension cell culture device being synchronously administered of the utility model, the suspension cell culture device
Mainly include 3 taper blake bottles (1,8,15), 3 delivery catheters (7,14,21), 8 sealing fastening strip (22,23,24,29,
28th, 31,32,34), 4 connecting conduits (25,26,27,33), 1 cylinder of delivery device 36,3 of cross four-way pipe 30,1
Rotor (46,48,51), 1 timer 45 of mini-sized blender 0,1 of thermal insulation film 49,1.
The volume of wherein taper blake bottle (1,8,15) is 150mL, and taper blake bottle (1,8,15) inner surface has silicon hydroxyl
There are taper blake bottle scale value (44,47,50), taper blake bottle in base (2,9,16), taper blake bottle (1,8,15) outer surface
(1,8,15) bottom of bottle places cylindrical rotor (46,48,51), is taper culture bottle cap above taper blake bottle (1,8,15)
(3,10,17) have one layer of air-permeative filter film between taper blake bottle (1,8,15) bottle and taper culture bottle cap (3,10,17)
(4,11,18), the local bottle cap corresponding to the air-permeative filter film (4,11,18) are engraved structure.
As shown in Fig. 2, there is the latex aperture (58,59,60) that can be stretched in taper culture bottle cap (3,10,17) center,
Latex aperture (58,59,60) is inserted into for the administration needle head (5,12,19) of delivery catheter (7,14,21).As shown in Figure 1, administration needle
Head (5,12,19) side is administration needle handle (6,13,20), and administration needle head (5,12,19) is followed by delivery catheter (7,14,21),
Delivery catheter (7,14,21) is connected to connecting conduit (25,26,27) by sealing fastening strip (22,23,24), and connection is led
Pipe (25,26,27) is connected to the port of export a, b, c of cross four-way pipe 30, junction have sealing fastening strip (29,28,
31) it is sealed, the arrival end d of cross four-way pipe 30 is connected to connecting conduit 33, and junction has sealing fastening strip 32 to carry out
Sealing, connecting conduit 33 connect the port of export f of delivery device 36, and junction has sealing fastening strip 34 to be sealed, before delivery device 36
Face exports 35 for delivery device, is delivery device port of export f before delivery device outlet 35,36 outer surface of delivery device is delivery device scale
Value 52, delivery device 36 are internally provided with circular plug 37, and circle plug 37 is followed by circular pin 38, the ellipse handle below of circular pin 38
43, there is wavy rack 42 in 38 one side of circular pin, and wavy rack 42 is closely engaged with manual gear 41, in manual gear 41
The heart is fixing axle 40, and fixing axle 40 is located at fixed 39 one end of short slab, and fixed 39 other end of short slab is connected to 36 afterbody one of delivery device
Side, taper blake bottle (1,8,15) have thermal insulation film 49 below, and thermal insulation film 49 is below mini-sized blender 0, miniature to stir
Mixing 0 surface of device has timer 45, and 45 surface of timer has timer display screen 53, timer switch button 54, timer to advance
Button 55, timer backspace button 56, Timer modes button 57, as shown in Figure 3.
During the utility model use, taper blake bottle (1,8,15) can be used to carry out a variety of suspension cells same
Stepization is cultivated and a variety of suspension cell cultures are in good condition, this with the utility model special designing taper blake bottle (1,8,
15), delivery catheter (7,14,21), sealing fastening strip (22,23,24,29,28,31,32,34), connecting conduit (25,26,27,
33), cross four-way pipe 30, delivery device 36, cylindrical rotor (46,48,51), thermal insulation film 49, mini-sized blender 0, fixed
When device 45 grade there is direct relation.
The specifically used flow of the utility model is as follows：
Suspension cell A, B, C are cultivated in taper blake bottle (1,8,15) respectively, it is necessary to be synchronized to suspension cell A, B, C
Change administration, need experimenter's right hand turn manual gear 41 clockwise at this time, strength is passed to wavy tooth by manual gear 41
Item 42, wavy rack 42 are located on circular pin 38, drive circular 37 movement of plug by the movement of circular pin 38, circle plug 37 pushes away
Drug in dynamic delivery device 36 passes through delivery device outlet 35, delivery device port of export f, connecting conduit 33, cross four-way pipe 30
Arrival end d is pushed ahead, and can pass through port of export a, connecting conduit 25, delivery catheter 7, the administration needle head of cross four-way pipe 30
5, to A plant cell suspension cultures, can also lead by the port of export b of cross four-way pipe 30, connecting conduit 26, administration simultaneously
Pipe 14, administration needle head 12 to B plant cell suspension cultures, more can be simultaneously by the port of export c of cross four-way pipe 30, connection
Conduit 27, delivery catheter 21, administration needle head 19 to C plant cell suspension cultures, while realization to tri- kinds of cells of A, B, C to
Medicine.In cell culture simultaneously, it is necessary to mini-sized blender 0 be opened, to drive rotor to the culture in taper blake bottle (1,8,15)
Liquid is stirred, and avoids cell attachment.Final the utility model, which not only can be realized operatively, carries out a variety of suspension cells
It is conventional to cultivate and realized to the administration of a variety of synchronizing of suspension cell in right amount.
The description of the utility model is merely illustrative above, and not restrictive, those skilled in the art should be managed
Solution can carry out it within the spirit and scope of claim restriction many modifications, variation or equivalent modification, but they are all
It will fall into the scope of protection of the utility model.
Claims (10)
1. a kind of suspension cell culture device being synchronously administered, which is characterized in that including at least two taper blake bottles, administration
Device, more siphunculus and blender；
Each taper blake bottle includes bottle and bottle cap, and the bottle inner wall is equipped with hydrophobic layer, and the bottle cap is equipped with one
Hole；
The outlet of the delivery device is connected by connecting conduit with an entrance of more siphunculus, other outlets of more siphunculus are successively
By connecting conduit, delivery catheter, taper blake bottle bottle cap on hole connected with taper blake bottle；
The taper blake bottle is placed on blender, rotor is placed in conical flask, turn on agitator, rotor is in taper blake bottle
Interior rotation.
2. the suspension cell culture device as described in claim 1 being synchronously administered, which is characterized in that the taper blake bottle
An air-permeative filter film is equipped between bottle and bottle cap, the local bottle cap corresponding to the air-permeative filter film is engraved structure.
3. the suspension cell culture device as described in claim 1 being synchronously administered, which is characterized in that the hole is that can stretch
Latex aperture.
4. the suspension cell culture device as described in claim 1 being synchronously administered, which is characterized in that connecting conduit and more siphunculus
Junction, the junction of outlet of connecting conduit and delivery device, the junction of connecting conduit and delivery catheter pass through sealing
Fastening strip seals.
5. the suspension cell culture device as described in claim 1 being synchronously administered, which is characterized in that the end of the delivery catheter
It holds as an administration needle, the needle handle of the administration needle is arranged on outside bottle cap.
6. the suspension cell culture device as described in claim 1 being synchronously administered, which is characterized in that the delivery device is piston
Syringe, including delivery device outlet, to cylindrantherae and piston；
The piston includes the circular plug, intermediate circular pin and the oval handle of end of front end, and circle plug and circular pin are located at
To in cylindrantherae.
7. the suspension cell culture device as claimed in claim 6 being synchronously administered, which is characterized in that the circular pin one side is set
There is wavy rack, described to be equipped with two protrusions to cartridge end, one of protrusion is equipped with one and fixes short slab, a manual gear
Fixing axle be fixed on the fixation short slab, and the gear is tightly engaged with the wavy rack on circular pin, rotate gear, will
Promote circular pin movement.
8. the as described in claim 1 suspension cell culture device being synchronously administered, which is characterized in that the mini-sized blender with
A thermal insulation film is equipped between taper blake bottle.
9. the suspension cell culture device as described in claim 1 being synchronously administered, which is characterized in that the mini-sized blender is set
There is timer, after which is equipped with timer display screen, timer switch button, timer forwarding button, timer
Move back button and Timer modes button.
10. the suspension cell culture device as described in claim 1 being synchronously administered, which is characterized in that the hydrophobic layer is silicon
Hydroxyl layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483610.7U CN207418767U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell culture device being synchronously administered |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483610.7U CN207418767U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell culture device being synchronously administered |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207418767U true CN207418767U (en) | 2018-05-29 |
Family
ID=62308267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721483610.7U Expired - Fee Related CN207418767U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell culture device being synchronously administered |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207418767U (en) |
-
2017
- 2017-11-08 CN CN201721483610.7U patent/CN207418767U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180529 Termination date: 20181108 |