CN207567242U - Convenient for the novel compositions culture bottle of dosing - Google Patents

Abstract

The utility model discloses a kind of novel compositions culture bottles convenient for dosing, match including triangle culture bottle, torticollis and support bottle, threeway plastic tube and administration instrument；The threeway plastic tube is connected respectively with triangle culture bottle and torticollis culture bottle；Administration needle is equipped on the triangle culture bottle and torticollis culture bottle, the administration needle is equipped with the syringe needle of porous structure.The utility model is conducive to synchronize suspension cell and attached cell administration and suspension cell and attached cell can be administered alone respectively, help to carry out suspension cell and attached cell artificial controllably manual administration and ensure that injection speed is at the uniform velocity constant, help to cultivate suspension cell and attached cell simultaneously and ensure that suspension cell and attached cell are in sterile ventilative basic cultivation conditions, it is final to realize the synchronization that pharmaceutical intervention is carried out to suspension cell and attached cell, at the uniform velocity change, controllableization and guarantee complete suspension cell and attached cell sterile, ventilative, suitable routine culture.

Description

Convenient for the novel compositions culture bottle of dosing

Technical field

The utility model is related to cell culture technologies, and in particular to a kind of novel compositions culture bottle convenient for dosing.

Background technology

Cell culture technology (cell culture technology) is INVENTIONModern cell, toxicology, physiology, drug effect One of routine techniques, the technology be broadly divided into suspended cell culture technic, attached cell culture technique (Song Hong, Han Yunzhu, Progress [J] Zunyi Medical College journal of Cai Guohui cell culture technologies, 2014,37 (5)：549-554.), wherein suspending Cell culture technology requirement suspension cell is in suspended state, and attached cell culture technique requirement attached cell is in adherent shape State, such as the associated culture techniques of the leukaemia cell of the normal lymphocyte of body or exception belong to suspension cell training The technology of supporting, and (Pei Fei, He Rui, Li Junyan wait bis- thin for the normal nerve cell of body, Skeletal Muscle Cell, vascular endothelial cell Born of the same parents plant retention rate [J] the China's Tissue Engineering Studies and clinical rehabilitation for improving artificial blood vessel's endothelial cell, 2010,14 (40)： The phase of the tumour cells such as associated culture techniques or abnormal breast cancer cell, lung carcinoma cell, stomach cancer cell 7505-7508.) It closes culture technique and belongs to attached cell culture technique.

In cell culture technology, need to carry out suspension cell and attached cell routine culture (account for modern Shun, Xing Yueteng, Application study progress [J] the feeds for opening refined cell culture technologies are read extensively, and 2013, (1)：8-11.), and to implement thin to suspending The reasonable administration processing of born of the same parents and attached cell, and in existing suspension cell and attached cell culture technique, it can usually encounter thin Bacterium, virus, mycoplasma, Chlamydia, fungi etc. pollution condition, therefore, existing suspension cell and attached cell culture technique Lack for suspension cell and the synchronization drug delivery system of attached cell, how controllably change drug delivery system, sterilization drug delivery system Improve suspension cell and attached cell cell culture technology, administration processing, avoid pollution be INVENTIONModern cell, toxicology, life Of science, pharmacodynamics etc. very important research topic.

Invention content

For the defects in the prior art, the purpose of this utility model is to provide one kind can solve suspension cell and patch The cell culture of parietal cell combination culture bottle, avoids the novel compositions convenient for dosing of the relevant issues such as pollution from training at administration processing Support bottle.

To achieve the above object, the utility model uses following technical scheme：

A kind of novel compositions culture bottle convenient for dosing, which is characterized in that including：

One triangle culture bottle, a torticollis, which are matched, supports bottle, a threeway plastic tube and an administration instrument；The three-way plastic Expects pipe has threeway plastic tube entrance, towards the outlet of triangle culture bottle and the outlet towards torticollis culture bottle；Threeway plastic tube Entrance is connected by entrance plastic union pipe with administration instrument, and the outlet towards triangle culture bottle passes through the first delivery tube It is connected with triangle culture bottle administration needle, the outlet towards torticollis culture bottle is given by the second delivery tube and torticollis culture bottle Medicine needle is connected；The triangle culture bottle administration needle passes through the triangle culture bottle cap being set on triangle culture bottle to penetrate with triangle training It supports in bottle, the torticollis culture bottle administration needle passes through the torticollis culture bottle cap being set on torticollis culture bottle to penetrate with torticollis culture In bottle；Triangle culture bottle administration needle and torticollis culture bottle administration needle are equipped with the syringe needle of porous structure.

Wherein, the volume of the triangle culture bottle is 150mL, and outer surface is equipped with scale value, and inner surface is equipped with thickness Silicone hydroxyl hydrophobic film for 0.04mm；The floor space of the torticollis culture bottle is 44cm2, and outer surface is equipped with scale value；Institute The lower section for stating triangle culture bottle is equipped with insulating sheets, and the lower section of the insulating sheets is equipped with miniature tumbler, the torticollis training The lower section for supporting bottle is equipped with culture plate.

Wherein, first delivery tube and towards between the outlet of triangle culture bottle be equipped with the first plastic union pipe；It is described Second delivery tube and towards between the outlet of torticollis culture bottle be equipped with the second plastic union pipe；First plastic union pipe is given with first The junction of pencil, the first plastic union pipe with towards the junction of outlet of triangle culture bottle, the second plastic union pipe with it is logical Toward the junction of outlet, the junction of the second plastic union pipe and the second delivery tube, entrance plastic union pipe of torticollis culture bottle It is equipped with what is sealed with the junction and the junction of entrance plastic union pipe and threeway plastic tube entrance that instrument is administered Rubber is buckled.

Wherein, the triangle culture bottle cap is equipped with multiple irregular shape air holes, and center is equipped with the first elasticity breast Glue aperture, the triangle culture bottle administration needle pass through the described first elastic aperture to be deep into triangle culture bottle；The torticollis training It supports bottle cap and is equipped with multiple irregular shape air holes, center is equipped with the second elastic emulsion aperture, and the torticollis culture bottle is given Medicine needle passes through the described second elastic aperture to be deep into torticollis culture bottle.

Wherein, it is respectively provided with an elasticity of plastics Small clamp on first plastic union pipe and the second plastic union pipe.

Wherein, it is equipped with double-deck air filtration film in the triangle culture bottle cap and the torticollis culture bottle cap；It is described The tunica fibrosa that double-deck air filtration film has 0.22 μ m diameter micropore by two layers forms.

Wherein, first delivery tube is equipped with the first delivery tube filter and the first administration tube valve, the first delivery tube Valve is equipped with plastics knob；Second delivery tube be equipped with the second delivery tube filter and second administration tube valve, second Tube valve is administered and is equipped with plastics knob；Be equipped in first delivery tube filter and the second delivery tube filter two layers 0.22 μm Microporous fiber membranes.

Wherein, administration needle of the triangle culture bottle administration needle for no bending angle；The torticollis culture bottle administration needle point For connected leading portion and back segment, the back segment is connected with the second delivery tube, and the length of the leading portion and the back segment is respectively 2.5cm and 4cm, the angle between the leading portion and back segment are 165 degree.

Wherein, the outer surface of the administration instrument is equipped with administration instrument scale marker, and administration instrument internal has cylindrical work Plug, cylindrical piston are connected with hand piston bar below, and the side of hand piston bar is equipped with plastics rack, plastics rack and one The gear teeth in circular wheel are meshed, and the center of the circular wheel is circular shaft, and circular shaft is located at one end of flat short slab, flat short The other end of plate is fixed on the tail portion of administration instrument, and hand piston rod rear end is connected with hand piston handle.

Compared with prior art, the beneficial benefit of the utility model is：Contribute to suspension cell and attached cell progress It is synchronous to be administered and suspension cell and attached cell are administered alone respectively, contribute to suspension cell and attached cell It carries out artificial controllably manual administration and ensures that injection speed is at the uniform velocity constant, help to cultivate suspension cell and attached cell simultaneously And ensure that suspension cell and attached cell be in sterile ventilative basic cultivation conditions, it is final realize to suspension cell and it is adherent carefully The synchronization of born of the same parents' progress pharmaceutical intervention, at the uniform velocity change, controllableization and guarantee complete suspension cell and attached cell sterile, ventilative , suitable routine culture.

Description of the drawings

Fig. 1 is the structure diagram of the utility model.

Fig. 2 is the structure diagram of triangle culture bottle cap.

Fig. 3 is the structure diagram of torticollis culture bottle cap.

Fig. 4 is the partial enlarged view of delivery tube plastic spike 4.

Fig. 5 is the partial enlarged view of delivery tube plastic spike 34.

Specific embodiment

A kind of novel compositions culture bottle convenient for dosing, including：Triangle culture bottle 1, delivery tube 9, is given torticollis culture bottle 33 Pencil 39, threeway plastic union pipe 16, plastic union pipe 13, plastic union pipe 43, plastic union pipe 18, administration instrument 20, every Hot thin plate 30, miniature tumbler 31 and culture plate 32；The volume of its intermediate cam culture bottle 1 has for 150mL and its outer surface Triangle culture bottle scale marker 3,1 inner surface of triangle culture bottle have silicone hydroxyl hydrophobic film 2 and its thickness 0.04mm, triangle culture Bottle 1 is put on insulating sheets 30 and 30 lower section of insulating sheets is miniature tumbler 31, and the rotating speed of miniature tumbler 31 is 75 Turn/min；1 top of triangle culture bottle is porous breathable bottle cap 5, and there are multiple irregular shape air holes on 5 surface of porous breathable bottle cap 46,5 inside of porous breathable bottle cap is containing double-deck air filtration film 6, the fiber that double-deck air filtration film 6 has 0.22 μm of micropore by two layers Film forms；The 5 flexible latex aperture 48 in center of porous breathable bottle cap, elastic emulsion aperture 48 have the first delivery tube plastic needle 7 to wear It crosses and the first delivery tube plastic needle 7 is no bending angle administration needle；Torticollis culture bottle 33 is lain against on culture plate 32, torticollis training The bottleneck of foster bottle 33 has porous breathable bottle cap 35 and porous breathable bottle cap 35 has multiple irregular shape air holes 47, bilayer Air filtration film 36, elastic emulsion aperture 49, the double-deck air filtration film 36 of porous breathable bottle cap 35 is two layers of 0.22 μm of micropores herein Tunica fibrosa has the second delivery tube plastic needle 37 to pass through in the elastic emulsion aperture 49 of porous breathable bottle cap 35, the modeling of the second delivery tube 37 leading portion of material pin and rear segment length are respectively 2.5cm and 4cm, and 37 leading portion of the second delivery tube plastic needle is bent clockwise with back segment Angle [alpha] is 165 degree, and back segment is connected with delivery tube 39.Delivery tube 9 is equipped with the first administration plastic needle 7,8 and of delivery tube filter Tube valve 10 is administered, delivery tube 39 is equipped with the second delivery tube plastic needle 37, administration filter 38 and administration tube valve 40.First The front end of 7 and second delivery tube plastic needle 37 of delivery tube plastic needle is respectively equipped with delivery tube plastic spike 4 and delivery tube plastic needle 34；Six delivery tube plastic spike apertures 52 for being divided to two groups of symmetric arrays are respectively equipped in delivery tube plastic spike 4；Delivery tube is moulded It is divided to the delivery tube plastic spike aperture 53 of two groups of symmetric arrays there are six being set on material pin head 34.Delivery tube filter 8 and delivery tube 2 layers of 0.22 μm of microporous fiber membranes are respectively equipped in filter 38, delivery tube valve knob 11 is equipped in administration tube valve 10；Administration Delivery tube valve knob 41 is equipped in tube valve 40.

Threeway plastic union pipe 16 has arrival end a, port of export b and port of export c；The end of delivery tube 9 and delivery tube 39 Port of export b and port of export c are connected to by plastic union pipe 13 and plastic union pipe 43 respectively, junction by rubber buckle 12, Rubber buckle 15, rubber buckle 42,45 sealing of rubber buckle, the plastic union pipe 13 being connect with the end of delivery tube 9 are equipped with Elasticity of plastics clip 13；The plastic union pipe 43 being connect with the end of delivery tube 39 is equipped with elasticity of plastics clip 44.

Arrival end a is connected to administration instrument 20 by plastic union pipe 18, and junction uses rubber buckle 17 and rubber card 19 sealing of button；The outer surface that instrument 20 is administered is equipped with administration instrument scale marker 21, and being administered inside instrument 20 has cylindrical piston 22, hand piston bar 24 is connected with behind cylindrical piston 22, the side of hand piston bar 24 is equipped with plastics rack 23, plastic teeth Item 23 is meshed with the gear 26 on circular gear 28, and the center of circular gear 28 is circular shaft 27, and circular shaft 27 is located at flat One end of short slab 25, the other end of flat short slab 25 are fixed on 20 tail portion of administration instrument, and 24 rear end of hand piston bar is connected with hand Piston handle 29.

It is as follows using idiographic flow：

During the utility model use, design triangle culture bottle, torticollis culture bottle, delivery tube, the connection of threeway plastics Pipe etc., primarily to realizing synchronizations, sterilization, controllably to suspension cell and/or attached cell under sterile culture environment Change, at the uniform velocity change administration.

If selection is administered suspension cell, need to be put into drug in administration instrument 20, and use elasticity of plastics 44 grip plastic connecting tube 43 of clip, and delivery tube valve knob 41 is screwed, unclamping plastics using elasticity of plastics clip 14 connects Pipe 13 and delivery tube valve knob 11 is unscrewed, under drive of the experimenter by circular gear 28,26 band of gear of circular gear 28 Dynamic plastics rack 23 moves, and then pushes hand piston bar 24 that cylindrical piston 22 is driven to move, drug by administration instrument 20, Plastic union pipe 18, arrival end a, port of export b, plastic union pipe 13, delivery tube 9, delivery tube filter 8, the modeling of the first delivery tube Material pin 7, delivery tube plastic spike 4, delivery tube plastic spike aperture 52, last drug enters plant cell suspension cultures.

If selection is administered attached cell, need to be put into drug in administration instrument 20, and use elasticity of plastics Clip 44 unclamps plastic union pipe 43 and unscrews delivery tube valve knob 41, uses 14 grip plastic connecting tube of elasticity of plastics clip 13 and delivery tube valve knob 11 is screwed, under drive of the experimenter by circular gear 28, the gear 26 of circular gear 28 drives Plastics rack 23 moves, and then pushes hand piston bar 24 that cylindrical piston 22 is driven to move, and drug is by administration instrument 20, modeling Expect connecting tube 18, arrival end a, port of export c, plastic union pipe 43, delivery tube 39, delivery tube filter 38, the modeling of the second delivery tube Material pin 37, delivery tube plastic spike 34, delivery tube plastic spike aperture 53, last drug enters attached cell cultivating system.

If suspension cell and/or synchronizing of attached cell is administered in selection, need to be put into administration instrument 20 Drug, under drive of the experimenter by circular gear 28, the gear 26 of circular gear 28 drives plastics rack 23 to move, and then push away Piston of starting bar 24 drives cylindrical piston 22 to move, and drug connects by administration instrument 20, plastic union pipe 18, threeway plastics The a ends of take over 16, the b ends of threeway plastic union pipe 16 and c ends, delivery tube 9, delivery tube filter 8, are given plastic union pipe 13 Pencil plastic needle 7, delivery tube plastic spike 4, delivery tube plastic spike aperture 52, last drug enters suspension cell and/or patch Parietal cell cultivating system.

Final the utility model can be realized to suspension cell and/or the administration of attached cell cultivating system and is administered basic Process can meet completely suspension cell and/or attached cell cultivating system it is sterile, ventilation.

Claims (9)

1. a kind of novel compositions culture bottle convenient for dosing, which is characterized in that including：

One triangle culture bottle, a torticollis, which are matched, supports bottle, a threeway plastic tube and an administration instrument；The threeway plastic tube With threeway plastic tube entrance, towards the outlet of triangle culture bottle and the outlet towards torticollis culture bottle；Threeway plastic tube entrance It is connected by entrance plastic union pipe with administration instrument, the outlet towards triangle culture bottle passes through the first delivery tube and three Angle culture bottle administration needle is connected, and the outlet towards torticollis culture bottle passes through the second delivery tube and torticollis culture bottle administration needle It is connected；The triangle culture bottle administration needle passes through the triangle culture bottle cap being set on triangle culture bottle to penetrate with triangle culture bottle In, the torticollis culture bottle administration needle passes through the torticollis culture bottle cap being set on torticollis culture bottle to penetrate with torticollis culture bottle In；Triangle culture bottle administration needle and torticollis culture bottle administration needle are equipped with the syringe needle of porous structure.

2. the novel compositions culture bottle according to claim 1 convenient for dosing, it is characterised in that：

The volume of the triangle culture bottle is 150mL, and outer surface is equipped with scale value, and it is 0.04mm's that inner surface, which is equipped with thickness, Silicone hydroxyl hydrophobic film；The floor space of the torticollis culture bottle is 44cm², outer surface is equipped with scale value；The triangle culture The lower section of bottle is equipped with insulating sheets, and the lower section of the insulating sheets is equipped with miniature tumbler, the lower section of the torticollis culture bottle Equipped with culture plate.

3. the novel compositions culture bottle according to claim 1 convenient for dosing, it is characterised in that：

First delivery tube and towards between the outlet of triangle culture bottle be equipped with the first plastic union pipe；Second delivery tube With towards between the outlet of torticollis culture bottle be equipped with the second plastic union pipe；The connection of first plastic union pipe and the first delivery tube Place, the first plastic union pipe with towards the junction of outlet of triangle culture bottle, the second plastic union pipe with towards torticollis culture The junction of outlet, the junction of the second plastic union pipe and the second delivery tube, entrance plastic union pipe and the administration instrument of bottle Junction and the junction of entrance plastic union pipe and threeway plastic tube entrance be equipped with the rubber to seal and be buckled.

4. the novel compositions culture bottle according to claim 1 convenient for dosing, it is characterised in that：

The triangle culture bottle cap is equipped with multiple irregular shape air holes, and center is equipped with the first elastic emulsion aperture, institute Triangle culture bottle administration needle is stated to be deep into triangle culture bottle across the described first elastic aperture；On the torticollis culture bottle cap Equipped with multiple irregular shape air holes, center is equipped with the second elastic emulsion aperture, and the torticollis culture bottle administration needle passes through institute The second elastic aperture is stated to be deep into torticollis culture bottle.

5. the novel compositions culture bottle according to claim 3 convenient for dosing, it is characterised in that：

An elasticity of plastics Small clamp is respectively provided on first plastic union pipe and the second plastic union pipe.

6. the novel compositions culture bottle according to claim 4 convenient for dosing, it is characterised in that：

Double-deck air filtration film is equipped in the triangle culture bottle cap and the torticollis culture bottle cap；The bilayer air filtration is thin The tunica fibrosa that film has 0.22 μ m diameter micropore by two layers forms.

7. the novel compositions culture bottle according to claim 1 convenient for dosing, it is characterised in that：

First delivery tube is equipped with the first delivery tube filter and the first administration tube valve, and the first administration tube valve is equipped with Plastics knob；Second delivery tube is equipped with the second delivery tube filter and the second administration tube valve, the second administration tube valve It is equipped with plastics knob；Two layers of 0.22 μm of microporous fibres are equipped in first delivery tube filter and the second delivery tube filter Film.

8. the novel compositions culture bottle according to claim 1 convenient for dosing, it is characterised in that：

Administration needle of the triangle culture bottle administration needle for no bending angle；The torticollis culture bottle administration needle be divided into it is connected before Section and back segment, the back segment are connected with the second delivery tube, and the length of the leading portion and the back segment is respectively 2.5cm and 4cm, institute It is 165 degree to state the angle between leading portion and back segment.

9. the novel compositions culture bottle according to claim 1 convenient for dosing, it is characterised in that：

The outer surface of the administration instrument is equipped with administration instrument scale marker, and administration instrument internal has cylindrical piston, cylindrical It is connected with hand piston bar behind piston, the side of hand piston bar is equipped with plastics rack, on plastics rack and a circular wheel The gear teeth be meshed, the center of the circular wheel is circular shaft, and circular shaft is located at one end of flat short slab, and flat short slab is in addition The tail portion of administration instrument is fixed in one end, and hand piston rod rear end is connected with hand piston handle.