CN104726333B - Human pancreas cancer White Rabbit head of pancreas Transplanted tumor model builds and uses oncocyte preparation facilities - Google Patents
Human pancreas cancer White Rabbit head of pancreas Transplanted tumor model builds and uses oncocyte preparation facilities Download PDFInfo
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Abstract
Human pancreas cancer White Rabbit head of pancreas Transplanted tumor model builds and uses oncocyte preparation facilities, belong to medical imaging technology field, it is made up of mouth (1), oblique mouth (2), mouth neck (3), body (4), stirring extractor (5), control fluid valve (6), body cavity (7), culture plate (8), steam generator (21), it is characterized in that: a length of 6 18 centimetres of described culture plate (8), width is 6 18 centimetres, and height is 0.5 2 centimetres；Concave surface culture dish (10) nature of glass, top half cylindrical shape, the latter half is the culture dish concave bottom (33) of surface plate shape, and bottom center has ware bottom outlet (15).Culture plate (8) top is the culture plate vallecular cavity (31) that culture plate cell wall (32) surrounds, and inside fills water；Culture plate (8) bottom is steam zone of heating (12) at the bottom of ware.The method equipment therefor makes simple, and workable, with low cost, effect is obvious.
Description
Technical field
The present invention relates to human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities, belong to medical science shadow
As learning a skill field.
Background technology
Cancer of pancreas is the most dangerous a kind of, the digestive tract tumor of high malignancy, and its age of onset presents obvious year in recent years
Gently change trend.Report that its incidence and mortality is close to 1: 1 according to NIH.By pancreas anatomy and biology
The impact of the factors such as feature, when making a definite diagnosis, majority has been late period, and the chance of excision only has 15%～20% even lower, and
Postoperative easy relapse and metastasis.Early diagnosis is the key factor improving cancer of pancreas prognosis, the most still lacks a kind of non-invasive early stage and examines
Break and the effective ways of monitoring curative effect.The medication combined Chinese medicine of Common Chemotherapy is treated the most clinically, medicine, treatment side
Case is a lot, but still can not in molecular image level effective evaluation curative effect of medication.In recent years, molecular imaging has become as
The new direction of cancer of pancreas diagnosis and treatment, its application is increasingly extensive, wherein with ultra micro super-paramagnetism nano ferric oxide particles (USPIO) as base
The magnetic resonance molecular image of plinth is the research direction of a great potential, and USPIO is a kind of efficient solid tumor nuclear magnetic resonance
Contrast medium, it has distinguishes tumor tissues and the ability of normal structure, and this makes them in a certain kind tissue (tumor or normal group
Knit) in assemble more, thus cause at this T2 magnetic resonance signal intensity to lower, produce the effect of strengthening contrast.Super suitable with ultra micro
The most important step of magnetic resonance molecular image based on magnetic Nano ferric oxide particles (USPIO) builds human pancreas exactly
Cancer White Rabbit head of pancreas Transplanted tumor model.Particularly as follows: the cultivation of 1. human pancreas cancer cell strain PANC-1, BxPC-3: PANC-1 cell
Use the DMEM high glucose medium containing 10% import hyclone；BxPC-3 cell uses containing 10% import hyclone
RPMI-1640 culture medium.Condition of culture be 37 DEG C, saturated humidity, 5% carbon dioxide.Cell is adherent growth.Two kinds of cells
Often within 3-4 days, pass on 1 time by 1: 2.2. collection cell suspension: the above-mentioned cell of exponential phase, goes training in superclean bench
Supporting base, with aseptic PBS 2 times, each culture bottle adds 3ml 0.25% trypsinization liquid, goes to digest after cell rounding
Liquid, adds 3ml culture medium and terminates digestion, stretches into after culture bottle blows and beats bottle wall repeatedly with dropper, cell suspension is moved into 50ml from
In heart pipe.It is centrifuged 5 minutes with the rotating speed of 1000rpm, supernatant discarded culture medium.With serum-free medium, cell is hanged, adjust
Viable cell concentrations is to 108/ml.3. White Rabbit subcutaneous vaccination oncocyte: draw cell suspension with 1ml syringe, right in White Rabbit
Hypogastric region subcutaneous injection cell suspension 100uL (i.e. 1 × 107 cell/only).Use PANC-1 cell suspension inoculation White Rabbit 5
Only, BxPC cell suspension inoculation White Rabbit 5 is used.After 3-4 week, tumor body surface diameter length is to real for next step during about 1cm
Test.4. utilize the T2 (T2 and T2*) of magnetic resonance T2map and T2*map technical measurement tumor tissues, pass through disperse
Weighted imaging (DWI) measures its apparent diffusion coefficient (ADC), and hydrogen MRS (1HMRS) measures tumor tissues
Interior major metabolite wave spectrum.By area under the T2 of tumor tissues, apparent diffusion coefficient, metabolite peak ratio and peak
Than dyeing with its pathology HE and SABC index (Cyclin D1 propagation dyeing and the dyeing of Hoechst and Tunel apoptosis)
Carrying out check analysis, the microcosmic pathological change of research cancer of pancreas, including ECS, between apoptosis and propagation etc. and MRI
Relation.In above step, the cultivation of human pancreas cancer cell strain PANC-1, BxPC-3 is the most key and basic, at present everyone pancreas
The cultivation of adenocarcinoma cell strain is required on superclean bench carry out, and the medicine of needs is many, spends big, complex operation, Er Qiejing
Often resulting in bigger waste, exhaustless cell will be discarded.The most how to work out one to grasp in the external world
Make, the oncocyte cultivation preparation facilities of low cost becomes a great problem being badly in need of solving, so when cultivating oncocyte, will train
The cell supported is placed in suitable growth conditions, operates the parts of inside outside device, a kind of human pancreas cancer White Rabbit pancreas of invention
Head Transplanted tumor model structure oncocyte preparation facilities is necessary.
Summary of the invention
In order to overcome everyone cultivation of pancreas cancer cell strain in reality cell cultivation process to be required at superclean bench
On carry out, the medicine of needs is many, spends big, complex operation, and often results in a difficult problem for bigger waste, the invention provides
Human pancreas cancer White Rabbit head of pancreas Transplanted tumor model builds and uses oncocyte preparation facilities, this kind of human pancreas cancer White Rabbit head of pancreas transplanted tumor
Model construction oncocyte preparation facilities, first can prepare utensil by cell and be placed in inside, it is to avoid with extraneous contact can
Energy.Secondly, the vapor (steam) temperature that steam generator produces is higher, it is possible to carry out disinfection device inside before use, and permissible
Being passed through steam zone of heating at the bottom of ware, the water in heating culture plate, the cell for cultivating provides suitable growth temperature.Again, concave surface
The oncocyte that stirring extractor scrapes down can be gathered in concave surface culture dish diapire central authorities by gravity by culture dish automatically,
Can automatically pass on, eliminate the operation assembling cell with centrifuge, allow preparation cell not by hydrolytic enzyme and at a high speed from
The injury that its central band is come.Stirring extractor, while stirring, is combined with syringe, it is possible to quickly and efficiently by thin for the tumor for preparing
Born of the same parents are for the structure of human pancreas cancer White Rabbit head of pancreas Transplanted tumor model, thus reach to reduce medicine usage amount, reduce cost, simplify
The purpose of operation.
The technical solution adopted for the present invention to solve the technical problems is:
The present inventor's cancer of pancreas White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities is by mouth 1, oblique mouth 2, mouth
Neck 3, body 4, stirring extractor 5, control fluid valve 6, body cavity 7, culture plate 8, side liquid pipe 9, concave surface culture dish 10, whole liquid pipe 11, ware
Steam louvre 13, spoke liquid pipe 14, ware bottom outlet 15, fluid valve 16, main hydraulic pipe 17 at the bottom of end steam zone of heating 12, ware, enter and steam at the bottom of ware
Steam pipe 18, lower plate steam guiding tube 19, upper plate steam guiding tube 20, steam generator 21, heating steam cabin 22, panel 23, power line 24,
Power-line plug 25, water tank 26, water pipe 27, space bar 28, water tank with water mouth 29, culture dish chamber 30, culture plate vallecular cavity 31, cultivation
Board slot wall 32, culture dish concave bottom 33, culture dish wall 34, body roof 35, control liquid stream valve head spin slot 36, groove top isolating membrane 37, control liquid
Stream valve head 38, control liquid stream valve rod 39, the solid pawl 40 of control fluid valve, suction pipe closedtop 41, movable coil 42, flap handle 43, circle
Flap 44, tube sheet fix convex 45, coil bearing 46, draw tube chamber 47, draw tube wall 48, force-transmitting gear 49, stirring sheet support 50,
Stirring sheet 51, the absorption mouth of pipe 52, syringe needle 53, needle lumen 54, needle tubing wall 55, needle tubing wall scale 56, needle tubing wall keep off 57, push away liquid pipe
58, push away that liquid tube head 59, needle tubing be convex 60, cell enters pipe 61, cell suction pipe 62, syringe 63 are constituted, it is characterised in that: described
A length of 6-18 centimetre of culture plate 8, width is 6-18 centimetre, and height is 0.5-2 centimetre, including concave surface culture dish 10, culture plate
Steam zone of heating 12, main hydraulic pipe 17, fluid valve 16, spoke liquid pipe 14, side liquid pipe 9, whole liquid at the bottom of vallecular cavity 31, culture plate cell wall 32, ware
Pipe 11.Concave surface culture dish 10 nature of glass, top half cylindrical shape, the culture dish wall 34 that thickness is 1-3 millimeter surround, a diameter of
1-4 centimetre, height is 1-3 centimetre, and the space surrounded is culture dish chamber 30；The latter half is the culture dish concave bottom of surface plate shape
33, the maximum height of culture dish concave bottom 33 bottom center to bottom surface is 0.5-0.75 centimetre, and the hole of bottom center is ware bottom outlet 15,
A diameter of 3-10 millimeter in hole, the ware bottom outlet 15 being positioned on space bar 28 communicates with main hydraulic pipe 17；It is positioned at the bottom of the ware on body 4 diapire
Hole 15 is then enclosed.Culture plate 8 top is the culture plate vallecular cavity 31 that culture plate cell wall 32 surrounds, the cell wall nature of glass, and thickness is 1-3
Millimeter, height is 0.3-1.5 centimetre；Culture plate vallecular cavity 31, inside fills water.Culture plate 8 bottom is steam zone of heating 12 at the bottom of ware, two
Culture plate vallecular cavity 31 end being provided with steam louvre 13 at the bottom of ware between part, separates.Steam louvre more than 13 at the bottom of ware, distribution
Between concave surface culture dish 10.Main hydraulic pipe 17, fluid valve 16, spoke liquid pipe 14, side liquid pipe 9 and whole liquid pipe 11 are all nature of glass pipelines
Structure, caliber is 2-3 millimeter.
Described mouth 1, after Tissues of Human Adenocarcinoma of Pancreas block is put into, seals with commercially available sterilised membrane filter, circular, a diameter of 2-5 li
Rice.Described oblique mouth 2 both ends open size shape is identical, and the opening of outer end is mouth 1, and inner opening is also the collar extension of mouthful neck 3,
The line of interior collar extension root edge central authorities is horizontal by the angle of 10-30 °, wall thickness 1-3 millimeter.Described mouth neck 3 rear end is before body 4
End sends, and upper limb is concordant with body 4 upper surface, a length of 1.5-3 centimetre of central authorities to oblique mouth 2；Lower edge is concordant with space bar 28, in
Centre to a length of 2-4 centimetre of oblique mouth 2；The nature of glass, wall thickness 1-3 millimeter.Described body 4 cuboid, a length of 15-50 li
Rice, width is 8-24 centimetre, and height is 6-18 centimetre, the nature of glass, wall thickness 1-3 millimeter.Described body roof 35 is the top of body 4
Face, is dispersed with 2 stirring extractors 5 and 1 control fluid valve 6 above.Space in body 4 is body cavity 7, described body cavity 7 first half
Part is divided into upper and lower two parts, space bar 28 nature of glass by space bar 28, and thickness is 1-3 millimeter, corresponding each ware bottom outlet on it
1 hole it is respectively arranged with, a diameter of 3-10 millimeter in hole at 15；Described space bar 28 leading edge and 1 lateral margin are both connected on body 4 sidewall,
Being provided above with 1 culture plate 8, lower section is provided with 1 water tank 26 and 1 steam generator 21.The length of described water tank 26
For 4-12 centimetre, width is 3-9 centimetre, and height is 2-7 centimetre, and the space between space bar 28 is walked for main hydraulic pipe 17；Water
There is the circular water tank with water mouth 29 of an a diameter of 2-5 millimeter case 26 side；Water tank 26 opposite side has 1 a diameter of 2-5 milli
The water pipe 27 of rice, the water pipe 27 opening in water tank 26 is positioned at bottom water tank 26.
Described steam generator 21 is cylindric, a diameter of 2-3 centimetre, and height is 2-6 centimetre, including entering steam pipe at the bottom of ware
18, lower plate steam guiding tube 19, upper plate steam guiding tube 20, steam generator 21, heating are steamed cabin 22, panel 23, power line 24, power supply
Line plug 25 and water pipe 27.Power line 24 and power-line plug 25 are known extraneous power supply can be introduced steam generator 21
Parts, power line 24 is bifilar foreskin copper wire line, and power-line plug 25 is biphase or 3-phase plug.Power line 24 is connected to control
Making sheet 23.Heating cabin 22 of steaming is cylindric structure, and a diameter of 0.5-1 centimetre, height is 1-2 centimetre, and upper end is led with lower plate
Steam pipe 19 and upper plate steam guiding tube 20 communicate, and lower sides has the hole of 1 a diameter of 2-5 millimeter；Heating steams what cabin 22 was heated
Resistance wire position is to its upper.Upper plate steam guiding tube 20 and lower plate steam guiding tube 19 nature of glass or copper, a diameter of 1-3 millimeter, wall thickness
0.3-0.5 millimeter.Entering steam pipe 18 at the bottom of ware is that upper plate steam guiding tube 20 and lower plate steam guiding tube 19 are in steam zone of heating 12 at the bottom of ware
Continuity, a length of 0.5-2 centimetre.
Described stirring extractor 5 includes suction pipe closedtop 41, movable coil 42, flap handle 43, circular flap 44, pipe
Plate fix convex 45, coil bearing 46, draw tube chamber 47, draw tube wall 48, force-transmitting gear 49, stirring sheet support 50, stirring sheet 51,
Draw the mouth of pipe 52.Suction pipe closedtop 41 silica gel matter, tool elasticity.The movable stainless steel of coil 42, a length of 4-12 centimetre, a diameter of
5-10 millimeter, wall thickness 1-3 millimeter.Flap handle 43 is the outwardly directed round bar in circular flap 44 edge, a diameter of 0.5-1.5 li
Rice, a length of 3-5 centimetre, stainless steel, it is connected with circular flap 44 by known rotation silk or is welded directly together.Circular
The circular corrosion resistant plate that flap 44 is a diameter of 2-5 centimetre, thickness is 1-5 millimeter, it is solid that central authorities have an inner surface to have tube sheet
The hole of fixed convex 45, fixes convex 45 by the tube sheet of engagement concavo-convex in this hole, it is possible to by tight with movable coil 42 for circle flap 44
Link together.The inner chamber of suction pipe is to draw tube chamber 47, the absorption tube wall 48 of stainless steel surround, and lower ending opening is for drawing
The mouth of pipe 52.Force-transmitting gear 49 be inclined-plane be the gear of 45 °.Stirring sheet support 50 is long in movable coil 42 least significant end vertical direction
The cross bar of cube shape, embeds fan-shaped rubber mass stirring sheet 51 base portion.Stirring sheet 51 rubber mass, fan-shaped, the widest 0.5-1 centimetre, base
Portion is finally 0.5-0.75 centimetre, a length of 0.5-2 centimetre.
Described control fluid valve 6 includes controlling liquid stream valve head spin slot 36, groove top isolating membrane 37, control liquid stream valve head 38, control liquid stream
Valve rod 39, the solid pawl 40 of control fluid valve.Control liquid stream valve head spin slot 36 is cylinder shape groove, cylindrical, a diameter of 1-3 centimetre, the degree of depth
For 3-5 millimeter.Groove top isolating membrane 37 is the flexible silicon colloid membrane of control liquid stream valve head spin slot 36 sealing, and thickness is 0.1-1 millimeter.
Control liquid stream valve head 38 is the cuboid stock of stainless steel, and a length of 0.8-2.8 centimetre, height and width are 2-5 millimeter,
Center weldment has the control liquid stream valve rod 39 of round bar shape.Control liquid stream valve rod 39 circular bar shape, a length of 4-8 centimetre, a diameter of 2-5 milli
Rice.Control liquid stream valve rod 39 lower end is welded on the solid pawl 40 of control fluid valve.Solid pawl 40 cuboid of control fluid valve, lower surface inwardly has
Cuboid is incised.
Described syringe 63 is kept off 57 by syringe needle 53, needle lumen 54, needle tubing wall 55, needle tubing wall scale 56, needle tubing wall, is pushed away liquid
Pipe 58, push away that liquid tube head 59, needle tubing are convex 60, cell enters pipe 61, cell suction pipe 62 forms.Syringe needle 53, needle lumen 54, needle tubing wall
55, needle tubing wall scale 56, needle tubing wall gear 57, push away liquid pipe 58, push away the structure of liquid tube head 59 and needle tubing convex 60 with known commercially available note
Emitter, it is cylindric that cell enters pipe 61, and wall thickness 1-2 millimeter, tube cavity diameter 1-3 millimeter, length 5-8 millimeter, the nature of glass, end is slightly
Expanding, wall thickness increases 0.1-0.5 millimeter.
Beneficial effects of the present invention is, human pancreas cancer White Rabbit head of pancreas Transplanted tumor model builds and uses oncocyte preparation facilities,
First cell can be prepared utensil and be placed in inside, it is to avoid with the possibility of extraneous contact.Secondly, the steaming that steam generator produces
Stripping temperature is higher, it is possible to carry out disinfection device inside before use, and can be passed through steam zone of heating at the bottom of ware, and heating is cultivated
Water in plate, the cell for cultivating provides suitable growth temperature.Again, stirring can be inhaled by concave surface culture dish by gravity
Take the oncocyte that device scrapes down and be automatically gathered in concave surface culture dish diapire central authorities, it is possible to automatically pass on, eliminate with from
The operation of cell is assembled in scheming, allows the injury that do not brought by hydrolytic enzyme and high speed centrifugation of cell of preparation.Stirring extractor is stirring
While mixing, be combined with syringe, it is possible to quickly and efficiently the oncocyte prepared is used for human pancreas cancer White Rabbit head of pancreas and moves
Plant the structure of tumor model, thus reach to reduce medicine usage amount, reduce cost, simplify the purpose of operation.Human pancreas cancer White Rabbit
Head of pancreas Transplanted tumor model builds and makes simply of oncocyte preparation facilities equipment therefor, and workable, with low cost, effect is bright
Aobvious.
Accompanying drawing explanation
The present invention is further described below in conjunction with the accompanying drawings.
Fig. 1 is the overall structure of the present inventor's cancer of pancreas White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities
Schematic diagram.
Fig. 2 is that the cultivation of the present inventor's cancer of pancreas White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities is hardened
Structure schematic diagram.
Fig. 3 is the control fluid valve of the present inventor's cancer of pancreas White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities
Vertical section structure schematic diagram.
Fig. 4 is that the stirring of the present inventor's cancer of pancreas White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities is drawn
Device vertical section structure schematic diagram.
Fig. 5 is that the syringe of the present inventor's cancer of pancreas White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities is whole
Body structural representation.
In figure 1. mouthfuls, 2. oblique mouth, 3. mouthful neck, 4. body, 5. stirring extractor, 6. control fluid valve, 7. body cavity, 8. culture plate,
9. side liquid pipe, 10. concave surface culture dish, 11. whole liquid pipes, steam zone of heating at the bottom of 12. wares, steam louvre at the bottom of 13. wares, 14. spoke liquid
Pipe, 15. ware bottom outlets, 16. fluid valves, 17. main hydraulic pipes, 18. enter steam pipe at the bottom of ware, 19. lower plate steam guiding tubes, 20. upper plate steam guiding tubes,
21. steam generators, 22. heat cabin of steaming, 23. panels, 24. power lines, 25. power-line plugs, 26. water tanks, 27. water
Pipe, 28. space bars, 29. water tank with water mouths, 30. culture dish chambeies, 31. culture plate vallecular cavities, 32. culture plate cell walls, 33. culture dishs
Concave bottom, 34. culture dish walls, 35. body roofs, 36. control liquid stream valve head spin slots, 37. groove top isolating membrane, 38. control liquid stream valve heads, 39.
Control liquid stream valve rod, the 40. solid pawls of control fluid valve, 41. suction pipe closedtops, 42. movable coils, 43. flap handles, 44. circular flaps,
45. tube sheets are fixed convex, 46. coil bearings, 47. absorption tube chambers, 48. absorption tube walls, 49. force-transmitting gear, 50. stirring sheet supports,
51. stirring sheets, 52. draw the mouth of pipe, 53. syringe needles, 54. needle lumen, 55. needle tubing walls, 56. needle tubing wall scales, and 57. needle tubing walls keep off,
58. push away liquid pipe, and 59. push away liquid tube head, and 60. needle tubings are convex, and 61. cells enter pipe, 62. cell suction pipes, 63. syringes.
Detailed description of the invention
Embodiment one:
As it can be seen, the human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities of the present invention is concrete
For: human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities is to be isolated from the outside by Tissue Culture Plate,
Cultured tissue and cell can be taken in extraneous natural environment by the temperature constant at culture dish on water-bath maintenance culture plate
Device freely, main body is transparent vitreous, by mouth 1, oblique mouth 2, mouth neck 3, body 4, stirring extractor 5, control fluid valve 6, body cavity
7, steam louvre 13, spoke at the bottom of steam zone of heating 12, ware at the bottom of culture plate 8, side liquid pipe 9, concave surface culture dish 10, whole liquid pipe 11, ware
Liquid pipe 14, ware bottom outlet 15, fluid valve 16, main hydraulic pipe 17, enter steam pipe 18 at the bottom of ware, lower plate steam guiding tube 19, upper plate steam guiding tube 20, steam
Vapour generator 21, heating are steamed cabin 22, panel 23, power line 24, power-line plug 25, water tank 26, water pipe 27, space bar
28, water tank with water mouth 29, culture dish chamber 30, culture plate vallecular cavity 31, culture plate cell wall 32, culture dish concave bottom 33, culture dish wall 34,
Body roof 35, control liquid stream valve head spin slot 36, groove top isolating membrane 37, control liquid stream valve head 38, control liquid stream valve rod 39, the solid pawl of control fluid valve
40, suction pipe closedtop 41, movable coil 42, flap handle 43, circular flap 44, tube sheet are fixed convex 45, coil bearing 46, are drawn
Tube chamber 47, absorption tube wall 48, force-transmitting gear 49, stirring sheet support 50, stirring sheet 51, the absorption mouth of pipe 52, syringe needle 53, needle lumen
54, needle tubing wall 55, needle tubing wall scale 56, needle tubing wall gear 57, push away liquid pipe 58, push away that liquid tube head 59, needle tubing be convex 60, cell enters pipe 61,
Cell suction pipe 62, syringe 63 form.Mouth 1 is that human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte prepares dress
Put the opening communicated with the external world, after Tissues of Human Adenocarcinoma of Pancreas block is put into, seal with commercially available sterilised membrane filter, thus ensure extraneous thin
Outside bacterium is shielded in, circular, a diameter of 2-5 centimetre.Tiltedly mouth 2 is Tissues of Human Adenocarcinoma of Pancreas block and the passage of filtrated air turnover, two
End opening size shape is identical, and the opening of outer end is mouth 1, and inner opening is also the collar extension of mouthful neck 3, interior collar extension root edge central authorities
Line is horizontal by the angle of 10-30 °, wall thickness 1-3 millimeter.Mouthful neck 3 is the part being connected between oblique mouth 2 and body 4, rear end from
Body 4 front end sends, and upper limb is concordant with body 4 upper surface, a length of 1.5-3 centimetre of central authorities to oblique mouth 2；Lower edge is flat with space bar 28
Together, central authorities to a length of 2-4 centimetre of oblique mouth 2；The nature of glass, wall thickness 1-3 millimeter.Body 4 cuboid, a length of 15-50 centimetre,
Width is 8-24 centimetre, and height is 6-18 centimetre, the nature of glass, wall thickness 1-3 millimeter.Body roof 35 is the end face of body 4, upper EDS maps
2 stirring extractors 5 and 1 control fluid valve 6.Space in body 4 is body cavity 7, and body cavity 7 first half is divided into by space bar 28
Two parts up and down, space bar 28 nature of glass, thickness is 1-3 millimeter, and it is respectively arranged with 1 hole at corresponding each ware bottom outlet 15, hole
A diameter of 3-10 millimeter, is that main hydraulic pipe 17 passes the passage that space bar 28 connects with ware bottom outlet 15；Space bar 28 leading edge and 1 lateral margin
Being both connected on body 4 sidewall, be provided above with 1 culture plate 8, lower section is provided with 1 water tank 26 and 1 steam generator 21.
A length of 4-12 centimetre of water tank 26, width is 3-9 centimetre, and height is 2-7 centimetre, and the space between space bar 28 is for main liquid
Pipe 17 is walked；There is the circular water tank with water mouth 29 of an a diameter of 2-5 millimeter water tank 26 side, after water injects water tank 26,
This mouthful of covered closing；Water tank 26 opposite side has the water pipe 27 of 1 a diameter of 2-5 millimeter, the water pipe 27 opening position in water tank 26
Bottom water tank 26, it is possible to the water of water tank 26 is transported in steam generator 21, due to top in being steamed in cabin 22 in heating
When water constantly reduces because being become the steam that can be released by the Resistant heating at this, it is possible to contain by steam generator 21
The negative pressure that hydrospace produces, is drawn onto the water in water tank 26 in steam generator 21, as long as so having water in ensureing water tank, so that it may
To produce steam continually.Steam generator 21 is the steam producing heat, is passed through steam zone of heating 12 at the bottom of ware to maintain
The device of culture plate 8 constant temperature water bath, cylindric, a diameter of 2-3 centimetre, height is 2-6 centimetre, including enter steam pipe 18 at the bottom of ware,
Lower plate steam guiding tube 19, upper plate steam guiding tube 20, steam generator 21, heating are steamed cabin 22, panel 23, power line 24, power line
Plug 25 and water pipe 27.Power line 24 and power-line plug 25 are known extraneous power supply can be introduced steam generator 21
Parts, power line 24 is bifilar foreskin copper wire line, and power-line plug 25 is biphase or 3-phase plug.Power line 24 is connected to control
Plate 23, panel 23 is with the panel on known water heater, it is possible to the temperature of water-bath water in display electrical condition and culture plate 8.
Heating cabin 22 of steaming is cylindric structure, and a diameter of 0.5-1 centimetre, height be 1-2 centimetre, upper end and lower plate steam guiding tube 19 and
Upper plate steam guiding tube 20 communicates, and lower sides has the hole of 1 a diameter of 2-5 millimeter, for the water in steam generator 21 water space
Enter heating to steam cabin 22；Heat the resistance wire position heated in cabin 22 of steaming to its upper, owing to specific heat of water is relatively big, top
Water is changed into after steam discharges, still to be supplemented into by the water in steam generator 21 water space.Upper plate leads vapour
Pipe 20 and lower plate steam guiding tube 19 are all that heating the steamed water vapour of heat that cabin 22 produces is transported to into steam pipe 18 at the bottom of ware, and then
Enter the pipeline of steam zone of heating 12, the nature of glass or copper, a diameter of 1-3 millimeter, wall thickness 0.3-0.5 millimeter at the bottom of ware.Enter at the bottom of ware
Steam pipe 18 is upper plate steam guiding tube 20 and the lower plate steam guiding tube 19 continuity in steam zone of heating 12 at the bottom of ware, a length of 0.5-2 li
Rice.Culture plate 8 is the parts cultivating, pass on and gather human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte, long
Degree is for 6-18 centimetre, and width is 6-18 centimetre, and height is 0.5-2 centimetre, including concave surface culture dish 10, culture plate vallecular cavity 31, training
Support steam zone of heating 12 at the bottom of board slot wall 32, ware, main hydraulic pipe 17, fluid valve 16, spoke liquid pipe 14, side liquid pipe 9, whole liquid pipe 11.Concave surface
Culture dish 10 nature of glass, top half cylindrical shape, the culture dish wall 34 that thickness is 1-3 millimeter surround, a diameter of 1-4 centimetre,
Highly being 1-3 centimetre, the space surrounded is culture dish chamber 30；The latter half is the culture dish concave bottom 33 of surface plate shape, cultivates
Ware concave bottom 33 bottom center is 0.5-0.75 centimetre to the maximum height of bottom surface, and the hole of bottom center is ware bottom outlet 15, hole straight
Footpath is 3-10 millimeter, and the ware bottom outlet 15 being positioned on space bar 28 communicates with main hydraulic pipe 17；It is positioned at the ware bottom outlet 15 on body 4 diapire then
It is enclosed.Culture plate 8 top is the culture plate vallecular cavity 31 that culture plate cell wall 32 surrounds, the cell wall nature of glass, and thickness is 1-3 millimeter,
Highly it is 0.3-1.5 centimetre；Culture plate vallecular cavity 31, inside fills water, it is possible to concave surface culture dish 10 is carried out water bath with thermostatic control.Culture plate
8 bottoms are steam zone of heating 12 at the bottom of ware, and culture plate vallecular cavity 31 end being provided with steam louvre 13 at the bottom of ware between two parts separates.
At the bottom of ware, steam louvre more than 13, are distributed between concave surface culture dish 10, are by heated water in steam zone of heating 12 at the bottom of ware
It is transported to the passage of culture plate vallecular cavity 31.When cultivating human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte, first exist
The culture plate 8 being positioned on space bar 28 cultivates human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte: human pancreas cancer
Cell strain PANC-1 and BxPC-3, PANC-1 cell uses the DMEM high glucose medium containing 10% import hyclone；BxPC-3
Cell uses the RPMI-1640 culture medium containing 10% import hyclone；Condition of culture be 37 DEG C, saturated humidity, 5% dioxy
Change carbon；Cell is adherent growth；Two kinds of cells pass on after cultivating 3-4 days.When passing on, first stir recessed with stirring extractor 5
The oncocyte of adherent growth in face culture dish 10, wait oncocyte by after at gravity sedimentation to ware bottom outlet 15, with control fluid valve 6 dozens
Drive fluid valve 16, in the case of need not being centrifuged, can be by main hydraulic pipe 17, fluid valve 16, spoke liquid pipe 14, side liquid pipe 9 and end
Cell is transferred to next culture plate 8 by liquid pipe 11.Main hydraulic pipe 17, fluid valve 16, spoke liquid pipe 14, side liquid pipe 9 and whole liquid pipe 11
Being all nature of glass pipeline configuration, caliber is 2-3 millimeter.Stirring extractor 5 includes suction pipe closedtop 41, movable coil 42, turns
Plate handle 43, circular flap 44, tube sheet fix convex 45, coil bearing 46, draw tube chamber 47, draw tube wall 48, force-transmitting gear 49,
Stirring sheet support 50, stirring sheet 51, the absorption mouth of pipe 52.Suction pipe closedtop 41 silica gel matter, tool elasticity, is movable coil 42 top
Medicated cap, it is possible to separated with the external world by movable coil 42, when drawing cell, the free-end of cell suction pipe 62 is enclosed within herein, i.e.
The needle lumen 54 of syringe 63 can be connected with drawing tube chamber 47.The movable stainless steel of coil 42, a length of 4-12 centimetre, diameter
For 5-10 millimeter, wall thickness 1-3 millimeter, it is possible to drawing the outer rotation of tube wall 48, driving force-transmitting gear 49 and stirring sheet support 50, will
The strength of operation flap handle 43 sends stirring sheet 51 to, plays the function of stirring.Flap handle 43 is circular flap 44 edge
Outwardly directed round bar, a diameter of 0.5-1.5 centimetre, a length of 3-5 centimetre, stainless steel, with circular flap 44 by known
Rotation silk connect or be welded directly together, in order to operator handle stirring extractor 5, by the oncocyte of adherent growth from institute
Scrape off on adherent, eliminate the use of hydrolytic enzyme.The circle that circular flap 44 is a diameter of 2-5 centimetre, thickness is 1-5 millimeter
Corrosion resistant plate, central authorities have an inner surface to have tube sheet to fix the hole of convex 45, fix convex by the tube sheet of engagement concavo-convex in this hole
45, it is possible to circle flap 44 is closely joined together with movable coil 42.Coil bearing 46 is in below circular flap 44
Reducing the parts of frictional resistance, structure is with commercially available bearing.The inner chamber of suction pipe is to draw tube chamber 47, by the absorption of stainless steel
Tube wall 48 surrounds, and regardless of how transferring is all an entirety, and its lower ending opening is for drawing the mouth of pipe 52, and under external force, tumor is thin
Born of the same parents can be inhaled into absorption tube chamber 47 from drawing the mouth of pipe 52 and leave body cavity 7.Force-transmitting gear 49 be inclined-plane be the gear of 45 °, its
Structure is with known gear, after two gear engagements, it is possible to the strength rotated transmitted to its vertical direction.Stirring sheet support
50 is the cross bar of cuboid in movable coil 42 least significant end vertical direction, embeds fan-shaped rubber mass stirring sheet 51 base portion, it is possible to
Support the stirring sheet 51 that tool is elastic.Stirring sheet 51 rubber mass, fan-shaped, the widest 0.5-1 centimetre, base portion is finally 0.5-0.75 li
Rice, a length of 0.5-2 centimetre, fan-shaped lower edge just matches with culture dish concave bottom 33, in order to reach best scraping effect.
Control fluid valve 6 is the parts controlling internal liquid valve 16 outside body roof 35, including control liquid stream valve head spin slot 36, the isolation of groove top
Film 37, control liquid stream valve head 38, control liquid stream valve rod 39, the solid pawl 40 of control fluid valve.Control liquid stream valve head spin slot 36 is cylinder shape groove, circle
Cylindricality, a diameter of 1-3 centimetre, the degree of depth is 3-5 millimeter, and control liquid stream valve head 38 can rotate around control liquid stream valve rod 39 wherein.Groove
Top isolating membrane 37 is the flexible silicon colloid membrane of control liquid stream valve head spin slot 36 sealing, and thickness is 0.1-1 millimeter, and effect is this to be opened
Mouth seals, it is ensured that the oncocyte do not cultivated in extraneous germ contamination body cavity 7.Control liquid stream valve head 38 is the cuboid of stainless steel
Shape stock, a length of 0.8-2.8 centimetre, height and width are 2-5 millimeter, and center weldment has the control liquid stream valve rod of round bar shape
39.Control liquid stream valve rod 39 circular bar shape, a length of 4-8 centimetre, a diameter of 2-5 millimeter.Control liquid stream valve rod 39 lower end is welded on control liquid
The solid pawl 40 of stream valve.Solid pawl 40 cuboid of control fluid valve, lower surface inwardly has cuboid and incises, and this is incised can be with liquid stream
The swing arm engagement of valve 16, when turn control liquid stream valve head 38, can be acted on by control liquid stream valve rod 39 and the solid pawl 40 of control fluid valve
In fluid valve 16, open or close the structure fluid valve 16 with known faucet.Syringe 63 is oncocyte vaccinization to be arrived
The utensil used during White Rabbit construct in vitro human pancreas cancer White Rabbit head of pancreas Transplanted tumor model, by syringe needle 53, needle lumen 54, needle tubing
Wall 55, needle tubing wall scale 56, needle tubing wall gear 57, push away liquid pipe 58, push away that liquid tube head 59, needle tubing are convex 60, cell enters pipe 61, cell is drawn
Pipe 62 forms.Syringe needle 53, needle lumen 54, needle tubing wall 55, needle tubing wall scale 56, needle tubing wall gear 57, push away liquid pipe 58, push away liquid tube head 59
The structure of convex with needle tubing 60 is with known commercially available syringe, simply after syringe needle 53 thrusts in White Rabbit body, if being drawn thin
During the amount deficiency of born of the same parents, then draw and need again to extract, but so increasing the danger of bacterial infection.So this
Bright cell of setting up on known syringe enters pipe 61 and cell suction pipe 62, and it is cylindric that cell enters pipe 61, wall thickness 1-2 millimeter, pipe
Intracavity diameter 1-3 millimeter, length 5-8 millimeter, the nature of glass, end slightly expands, and wall thickness increases 0.1-0.5 millimeter, in order to silica gel matter
Cell suction pipe 62 be enclosed within cell more securely and enter on pipe 61.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.The technology of the industry
Personnel, it will be appreciated that the present invention is not restricted to the described embodiments, simply illustrate this described in above-described embodiment and description
Bright principle, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes
Both falling within scope of the claimed invention with improvement, claimed scope is by its equivalence of appending claims
Thing defines.
Claims (2)
1. human pancreas cancer White Rabbit head of pancreas Transplanted tumor model builds and uses oncocyte preparation facilities, by mouth (1), oblique mouth (2), mouth neck
(3), body (4), stirring extractor (5), control fluid valve (6), body cavity (7), culture plate (8), side liquid pipe (9), concave surface culture dish
(10), steam louvre (13) at the bottom of steam zone of heating (12), ware at the bottom of whole liquid pipe (11), ware, spoke liquid pipe (14), ware bottom outlet (15),
Fluid valve (16), main hydraulic pipe (17), enter steam pipe at the bottom of ware (18), lower plate steam guiding tube (19), upper plate steam guiding tube (20), steam generation
Device (21), heating steam cabin (22), panel (23), power line (24), power-line plug (25), water tank (26), water pipe (27),
Space bar (28), water tank with water mouth (29), culture dish chamber (30), culture plate vallecular cavity (31), culture plate cell wall (32), culture dish are recessed
The end (33), culture dish wall (34), body roof (35), control liquid stream valve head spin slot (36), groove top isolating membrane (37), control liquid stream valve head
(38), control liquid stream valve rod (39), control fluid valve solid pawl (40), suction pipe closedtop (41), movable coil (42), flap handle
(43), circular flap (44), tube sheet are fixed convex (45), coil bearing (46), are drawn tube chamber (47), absorption tube wall (48), power transmission
Gear (49), stirring sheet support (50), stirring sheet (51), the absorption mouth of pipe (52), syringe needle (53), needle lumen (54), needle tubing wall
(55), needle tubing wall scale (56), needle tubing wall gear (57), push away liquid pipe (58), push away liquid tube head (59), needle tubing convex (60), cell enter pipe
(61), cell suction pipe (62), syringe (63) are constituted, it is characterised in that: a length of 6-18 centimetre described of culture plate (8),
Width is 6-18 centimetre, and height is 0.5-2 centimetre, including concave surface culture dish (10), culture plate vallecular cavity (31), culture plate cell wall
(32), steam zone of heating (12), main hydraulic pipe (17), fluid valve (16), spoke liquid pipe (14), side liquid pipe (9), whole liquid pipe at the bottom of ware
(11)；Concave surface culture dish (10) nature of glass, top half cylindrical shape, culture dish wall (34) that thickness is 1-3 millimeter surround, directly
Footpath is 1-4 centimetre, and height is 1-3 centimetre, and the space surrounded is culture dish chamber (30)；The latter half is the cultivation of surface plate shape
Ware concave bottom (33), the maximum height of culture dish concave bottom (33) bottom center to bottom surface is 0.5-0.75 centimetre, the hole of bottom center
For ware bottom outlet (15), a diameter of 3-10 millimeter in hole, the ware bottom outlet (15) being positioned on space bar (28) communicates with main hydraulic pipe (17)；
The ware bottom outlet (15) being positioned on body (4) diapire is then enclosed；Culture plate (8) top is the culture plate that culture plate cell wall (32) surrounds
Vallecular cavity (31), the cell wall nature of glass, thickness is 1-3 millimeter, and height is 0.3-1.5 centimetre；Culture plate vallecular cavity (31), inside fills water；
Culture plate (8) bottom is steam zone of heating (12) at the bottom of ware, is provided with the culture plate of steam louvre (13) at the bottom of ware between two parts
Vallecular cavity (31) end, separates；At the bottom of ware, steam louvre (13) is multiple, is distributed between concave surface culture dish (10)；Main hydraulic pipe (17), liquid
Stream valve (16), spoke liquid pipe (14), side liquid pipe (9) and whole liquid pipe (11) are all nature of glass pipeline configurations, and caliber is 2-3 millimeter；
Described mouth (1), after Tissues of Human Adenocarcinoma of Pancreas block is put into, seals with commercially available sterilised membrane filter, circular, a diameter of 2-5 centimetre；Institute
Oblique mouth (2) the both ends open size shape stated is identical, and the opening of outer end is mouth (1), outside inner opening also mouthful neck (3)
Mouthful, the line of interior collar extension root edge central authorities is horizontal by the angle of 10-30 °, wall thickness 1-3 millimeter；Described mouth neck (3) rear end from
Body (4) front end sends, and upper limb is concordant with body (4) upper surface, a length of 1.5-3 centimetre of central authorities to oblique mouth (2)；Lower edge and interval
Plate (28) is concordant, a length of 2-4 centimetre of central authorities to oblique mouth (2)；The nature of glass, wall thickness 1-3 millimeter；Described body (4) cuboid
Shape, a length of 15-50 centimetre, width is 8-24 centimetre, and height is 6-18 centimetre, the nature of glass, wall thickness 1-3 millimeter；Described body
Roof (35) is the end face of body (4), is dispersed with 2 stirrings extractor (5) and 1 control fluid valve (6) above；Sky in body (4)
Between be body cavity (7), described body cavity (7) first half is divided into upper and lower two parts by space bar (28), space bar (28) nature of glass,
Thickness is 1-3 millimeter, and on it, corresponding each ware bottom outlet (15) place is respectively arranged with 1 hole, a diameter of 3-10 millimeter in hole；Described interval
Plate (28) leading edge and 1 lateral margin are both connected on body (4) sidewall, are provided above with 1 culture plate (8), and lower section is provided with 1 water tank
(26) and 1 steam generator (21)；A length of 4-12 centimetre of described water tank (26), width is 3-9 centimetre, and height is 2-
Space between 7 centimetres, and space bar (28) is walked for main hydraulic pipe (17)；There is an a diameter of 2-5 millimeter water tank (26) side
Circular water tank with water mouth (29)；Water tank (26) opposite side has the water pipe (27) of 1 a diameter of 2-5 millimeter, and water pipe (27) exists
Opening in water tank (26) is positioned at water tank (26) bottom；Described steam generator (21) is cylindric, a diameter of 2-3 centimetre, high
Degree is for 2-6 centimetre, including entering steam pipe at the bottom of ware (18), lower plate steam guiding tube (19), upper plate steam guiding tube (20), steam generator
(21), steam cabin (22), panel (23), power line (24), power-line plug (25) and water pipe (27) are heated；Power line (24)
Being the known parts that extraneous power supply can introduce steam generator (21) with power-line plug (25), power line (24) is double
Stock foreskin copper wire line, power-line plug (25) is biphase or 3-phase plug；Power line (24) is connected to panel (23)；Add delivery in hot weather
Vapour cabin (22) is cylindric structure, a diameter of 0.5-1 centimetre, and height is 1-2 centimetre, upper end and lower plate steam guiding tube (19) and upper
Plate steam guiding tube (20) communicates, and lower sides has the hole of 1 a diameter of 2-5 millimeter；Heat the resistance wire of cabin (22) heating of steaming
Position is to its upper；Upper plate steam guiding tube (20) and lower plate steam guiding tube (19) nature of glass or copper, a diameter of 1-3 millimeter, wall thickness 0.3-
0.5 millimeter；Entering steam pipe at the bottom of ware (18) is that upper plate steam guiding tube (20) and lower plate steam guiding tube (19) are in steam zone of heating (12) at the bottom of ware
In continuity, a length of 0.5-2 centimetre；Described stirring extractor (5) include suction pipe closedtop (41), movable coil (42),
Flap handle (43), circular flap (44), tube sheet are fixed convex (45), coil bearing (46), are drawn tube chamber (47), absorption tube wall
(48), force-transmitting gear (49), stirring sheet support (50), stirring sheet (51), the absorption mouth of pipe (52)；Suction pipe closedtop (41) silica gel
Matter, tool elasticity；Movable coil (42) stainless steel, a length of 4-12 centimetre, a diameter of 5-10 millimeter, wall thickness 1-3 millimeter；Flap
Handle (43) is the outwardly directed round bar in circular flap (44) edge, a diameter of 0.5-1.5 centimetre, a length of 3-5 centimetre, stainless
Steel, is connected with circular flap (44) by known rotation silk or is welded directly together；Circular flap (44) is a diameter of 2-5
Centimetre, thickness be the circular corrosion resistant plate of 1-5 millimeter, central authorities have an inner surface to have tube sheet to fix the hole of convex (45), pass through
In this hole, the tube sheet of concavo-convex engagement fixes convex (45), it is possible to circle flap (44) and movable coil (42) are close-coupled at one
Rise；The inner chamber of suction pipe is to draw tube chamber (47), the absorption tube wall (48) of stainless steel surround, and lower ending opening is for drawing the mouth of pipe
(52)；Force-transmitting gear (49) be inclined-plane be the gear of 45 °；Stirring sheet support (50) is movable coil (42) least significant end vertical direction
The cross bar of upper cuboid, embeds fan-shaped rubber mass stirring sheet (51) base portion；Stirring sheet (51) rubber mass, fan-shaped, the widest 0.5-1
Centimetre, base portion is finally 0.5-0.75 centimetre, a length of 0.5-2 centimetre；Described control fluid valve (6) includes controlling the rotation of liquid stream valve head
Groove (36), groove top isolating membrane (37), control liquid stream valve head (38), control liquid stream valve rod (39), the solid pawl (40) of control fluid valve；Control fluid valve
Head spin slot (36) is cylinder shape groove, and cylindrical, a diameter of 1-3 centimetre, the degree of depth is 3-5 millimeter；Groove top isolating membrane (37) is control
The flexible silicon colloid membrane that liquid stream valve head spin slot (36) seals, thickness is 0.1-1 millimeter；Control liquid stream valve head (38) is rustless steel
The cuboid stock of matter, a length of 0.8-2.8 centimetre, height and width are 2-5 millimeter, and center weldment has the control of round bar shape
Liquid stream valve rod (39)；Control liquid stream valve rod (39) circular bar shape, a length of 4-8 centimetre, a diameter of 2-5 millimeter；Control liquid stream valve rod (39)
Lower end is welded on the solid pawl (40) of control fluid valve；Solid pawl (40) cuboid of control fluid valve, lower surface inwardly has cuboid and lacks
Carve.
Human pancreas cancer White Rabbit head of pancreas Transplanted tumor model structure oncocyte preparation facilities the most according to claim 1, its
It is characterised by: described syringe (63) is by syringe needle (53), needle lumen (54), needle tubing wall (55), needle tubing wall scale (56), needle tubing
Wall gear (57), push away liquid pipe (58), push away liquid tube head (59), needle tubing convex (60), cell enter pipe (61), cell suction pipe (62) composition；
Syringe needle (53), needle lumen (54), needle tubing wall (55), needle tubing wall scale (56), needle tubing wall gear (57), push away liquid pipe (58), push away liquid pipe
The structure of head (59) and needle tubing convex (60) is with known commercially available syringe, and it is cylindric that cell enters pipe (61), wall thickness 1-2 millimeter, pipe
Intracavity diameter 1-3 millimeter, length 5-8 millimeter, the nature of glass, end slightly expands, and wall thickness increases 0.1-0.5 millimeter.
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| CN109022258A (en) * | 2018-09-12 | 2018-12-18 | 哈尔滨师范大学 | Archeobacteria culture apparatus suitable for low-temperature micro-oxygen ambient growth |
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| CN113476082B (en) * | 2021-06-15 | 2022-10-21 | 齐齐哈尔医学院 | Fat enzymolysis gravity wax-sealing type automatic collector for rabbit pancreatic cancer cells |
Family Cites Families (3)
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| CN102335048B (en) * | 2011-07-18 | 2013-08-28 | 山西省肿瘤医院 | Modeling device for establishing bladder cancer orthotopic transplantation tumor animal model |
| CN104414769A (en) * | 2013-09-05 | 2015-03-18 | 孙高峰 | Method for establishing model of isolated VX2 transplanted tumor in rabbit lung by computer tomography (CT) guidance |
| CN103976803A (en) * | 2014-05-26 | 2014-08-13 | 贵阳医学院附属医院 | Human colon cancer nude mouse skin subcutaneous transplantation tumor model establishing method |
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2015
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022258A (en) * | 2018-09-12 | 2018-12-18 | 哈尔滨师范大学 | Archeobacteria culture apparatus suitable for low-temperature micro-oxygen ambient growth |
| CN109022258B (en) * | 2018-09-12 | 2022-06-10 | 哈尔滨师范大学 | Archaea culture device suitable for growth in low-temperature micro-aerobic environment |
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| Publication number | Publication date |
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| CN104726333A (en) | 2015-06-24 |
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