CN105838604A - Culture dish for mature adipocyte ceiling culture - Google Patents
Culture dish for mature adipocyte ceiling culture Download PDFInfo
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- CN105838604A CN105838604A CN201610330234.1A CN201610330234A CN105838604A CN 105838604 A CN105838604 A CN 105838604A CN 201610330234 A CN201610330234 A CN 201610330234A CN 105838604 A CN105838604 A CN 105838604A
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- culture dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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Abstract
The invention discloses a culture dish for mature adipocyte ceiling culture, and belongs to the technical field of biological medicine experimental devices. One or more inverted small culture dishes are arranged in an upright large culture dish; a U-shaped tube is arranged in the large culture dish; one end of each U-shaped tube is exposed outside the culture solution of the large culture dish; one end of each U-shaped tube is positioned inside each inverted small culture dish. No air is retained at the bottoms inside the inverted small culture dishes; meanwhile, exchange of liquids inside the inverted small culture dishes with external air can be promoted; meanwhile, the U-shaped tubes can be connected with a sampling device, and then mature adipocyte can be injected into the inverted small culture dishes for culture. The culture dish is reasonable in structural design, simple to operate, and capable of effectively culturing mature adipocyte.
Description
Technical field
The invention belongs to biological medicine experimental provision technical field, relate to a kind of culture dish, be specifically related to one
Plant the culture dish cultivated for mature fat cell ceiling.
Background technology
Adipose cell has important function in organism metabolism regulates and controls, and it serves not only as the storage of energy matter
Unit, and actively regulate the organism metabolism under Different Nutrition state by release active factors.Fat is thin
Born of the same parents' dysfunction is fat etc. as caused by lipid excess accumulation has a strong impact on human health.In vitro culture fat is thin
Born of the same parents and then to further investigate its function be the important content in current endocrine metabolism field.Because becoming
Ripe adipose cell contains triglyceride, and density is little compared with aqueous solution, is suspended in culture fluid surface in cultivation, because of
This, mature fat cell is cultivated and is used ceiling method more.But, existing carry out ceiling cultivation
Utensil is mainly the culture bottle of commercialization, there is not yet the culture dish cultivated for ceiling.Test at some
In, culture bottle is difficult to meet the operation requirement of experiment, and culture dish has irreplaceable advantage.
Therefore, a kind of culture dish that can be used in cultivating mature fat cell is needed badly.
Summary of the invention
It is an object of the invention to provide a kind of culture dish cultivated for mature fat cell ceiling, this training
Support ware reasonable in design, simple to operate, it is possible to effectively to cultivate mature fat cell.
The technical solution adopted in the present invention is, the culture dish cultivated for mature fat cell ceiling,
Including big culture dish and one or several little culture dish being inverted in big culture dish, also include one or number
Individual U-tube, U-tube one end is stretched in inverted little culture dish, and the other end stretches out in big culture dish to be cultivated
The surface of liquid.
The feature of the present invention also resides in,
The lower edge of little culture dish is placed in U-tube.
The caliber of U-tube is 1～3mm.
The sidewall of little culture dish is additionally provided with clamping part.
The opening of wide 2～4mm is offered at the ware mouth of little culture dish.
Described U-tube quantity is 3,3 U-tube equidistant arrangement.
U-tube is stretched out the one end on culture fluid surface and is connected with sample adding device.
Big culture dish top is additionally provided with culture dish top cover.
Compared with prior art, the present invention has a following useful technique effect:
Disclosed by the invention for mature fat cell ceiling cultivate culture dish, forward place big
Culture dish arranges one or several inverted little culture dish, also includes one or several U-tube, U-shaped
Pipe one end is exposed to outside the culture fluid of big culture dish, and one end is positioned at inverted little culture dish, it is ensured that be inverted
The inner bottom part of little culture dish without air remaining, can promote inverted little culture dish simultaneously in, liquid is with outer
Boundary's gas exchange.This culture dish reasonable in design, simple to operate, it is possible to effectively to cultivate ripe fat thin
Born of the same parents.
Further, the lower edge of little culture dish is placed in U-tube, owing to the caliber of U-tube is 1～3mm,
Therefore, from periphery there is 1 millimeter or several millimeters in big culture dish inner bottom to make the ware mouth of inverted little culture dish
Distance, make the adipose cell can gas exchange abundant with the external world, it is ensured that oxygen supply and CO2Discharge.
Further, the sidewall of inverted little cultivation is provided with clamping part, or offers at its ware mouth
Wide 2～the opening of 4mm, it is easy to clamping, convenient take out inverted little culture dish.Meanwhile, peripheral big training
The bottom supporting ware and the little culture dish of internal inversion is the thinnest, it is easy to the observation of cell.
Further, U-tube is stretched out the one end on culture fluid surface and is connected with sample adding device, convenient to inversion
Little culture dish carry out the conveying of adipose cell and culture fluid.
Accompanying drawing explanation
Fig. 1 is the structural representation of the embodiment of the present invention 1.
Wherein: 1. big culture dish, the least culture dish, 3.U type pipe, 4. clamping part, 5. culture dish top cover.
Detailed description of the invention
The present invention is described in detail with detailed description of the invention below in conjunction with the accompanying drawings.
Embodiment 1
Seeing Fig. 1, a kind of culture dish cultivated for mature fat cell ceiling, including big culture dish
1 and be inverted in big culture dish 1 or several little culture dish 2, also include one or several U-shaped
Pipe 3, U-tube 3 one end is stretched in inverted little culture dish 2, and the other end stretches out in big culture dish to be cultivated
The surface of liquid.The lower edge of little culture dish 2 is placed in U-tube 3, and the caliber of U-tube 3 is 1～3mm,
U-tube 3 is stretched out the one end on culture fluid surface and is connected with sample adding device.Big culture dish 1 top is additionally provided with training
Support ware top cover 5.
The sidewall of little culture dish 2 is additionally provided with clamping part 4.Or, at the ware mouth of little culture dish 2
Offer the opening of wide 2～4mm.
Preferably, U-tube 3 quantity is 3,3 U-tube 3 equidistant arrangement.
The culture dish that the present invention cultivates for mature fat cell ceiling, in use:
Inverted one or several little culture dish 2 are positioned in big culture dish 1, it is ensured that U-tube 3 low side
It is placed in inverted little culture dish 2.Add culture fluid in big culture dish 1, until inverted little cultivation
Ware 2 is internal is completely filled with culture fluid.Sample adding device is used to be injected by 3 mature fat cells of U-tube
Cultivate in inverted little culture dish 2.
Claims (8)
1. the culture dish cultivated for mature fat cell ceiling, it is characterised in that include big culture dish
(1) and be inverted in big culture dish (1) or several little culture dish (2), also include one or
Several U-tube (3), it is interior that inverted little culture dish (2) is stretched in U-tube (3) one end, and the other end is stretched
Go out the surface of culture fluid in big culture dish.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, the lower edge of little culture dish (2) is placed in U-tube (3).
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, the caliber of U-tube (3) is 1～3mm.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levy and be, the sidewall of little culture dish (2) is additionally provided with clamping part (4).
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levy and be, at the ware mouth of little culture dish (2), offer the opening of wide 2～4mm.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, described U-tube (3) quantity is 3,3 U-tube (3) equidistant arrangement.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, U-tube (3) is stretched out the one end on culture fluid surface and is connected with sample adding device.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, big culture dish (1) top is additionally provided with culture dish top cover (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610330234.1A CN105838604B (en) | 2016-05-18 | 2016-05-18 | For the culture dish of mature fat cell ceiling culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610330234.1A CN105838604B (en) | 2016-05-18 | 2016-05-18 | For the culture dish of mature fat cell ceiling culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105838604A true CN105838604A (en) | 2016-08-10 |
| CN105838604B CN105838604B (en) | 2018-07-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610330234.1A Expired - Fee Related CN105838604B (en) | 2016-05-18 | 2016-05-18 | For the culture dish of mature fat cell ceiling culture |
Country Status (1)
| Country | Link |
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| CN (1) | CN105838604B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345205A (en) * | 2017-09-06 | 2017-11-14 | 阜阳师范学院 | A kind of mature fat cell dedifferentes the device and method of culture |
| CN110295110A (en) * | 2019-06-26 | 2019-10-01 | 吴威 | A kind of equipment increasing body fat cell survival quantity |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000083656A (en) * | 1998-09-09 | 2000-03-28 | Meiji Milk Prod Co Ltd | Precursor adipocyte strain |
| CN205774572U (en) * | 2016-05-18 | 2016-12-07 | 西安医学院 | A kind of culture dish cultivated for mature fat cell ceiling |
-
2016
- 2016-05-18 CN CN201610330234.1A patent/CN105838604B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000083656A (en) * | 1998-09-09 | 2000-03-28 | Meiji Milk Prod Co Ltd | Precursor adipocyte strain |
| CN205774572U (en) * | 2016-05-18 | 2016-12-07 | 西安医学院 | A kind of culture dish cultivated for mature fat cell ceiling |
Non-Patent Citations (2)
| Title |
|---|
| TAKAHIRO SAKUMA ET AL.: "Mature, Adipocyte Derived, Dedifferentiated Fat Cells Can Differentiate Into Smooth Muscle-Like Cells and Contribute to Bladder Tissue Regeneration", 《THE JOURNAL OF UROLOGY》 * |
| WEI ET AL.: "Bovine dedifferentiated adipose tissue (DFAT) cells", 《ADIPOCYTE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345205A (en) * | 2017-09-06 | 2017-11-14 | 阜阳师范学院 | A kind of mature fat cell dedifferentes the device and method of culture |
| CN110295110A (en) * | 2019-06-26 | 2019-10-01 | 吴威 | A kind of equipment increasing body fat cell survival quantity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105838604B (en) | 2018-07-06 |
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Granted publication date: 20180706 Termination date: 20190518 |