CN203754727U - Device for hair follicle culture - Google Patents
Device for hair follicle culture Download PDFInfo
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- CN203754727U CN203754727U CN201420164275.4U CN201420164275U CN203754727U CN 203754727 U CN203754727 U CN 203754727U CN 201420164275 U CN201420164275 U CN 201420164275U CN 203754727 U CN203754727 U CN 203754727U
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- hair follicle
- culture
- culture dish
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Abstract
The utility model provides a culture device for hair follicle culture, which comprises a culture dish 1, a culture dish cover plate 2 and a hair follicle carrier net 3, wherein the bottom surface of the culture dish 1 is coated with type-IV collagen, and the middle carrier net 3 can support the growth of single hair follicles. The device provided by the utility model is simple to make and good in air tightness and can effectively realize distinguished culture of single hair follicles.
Description
Technical field
The utility model relates to a kind of labware, specifically a kind of culture dish for hair follicle culture.
Background technology
In recent years, people have obtained preliminary progress to the research of hair follicle biology, increasing research direction is positioned mechanism, the biological study of hair follicle stem cells, the interaction mechanism between corium and epidermis and the research of hair follicle growth Regulation Mechanism of hair follicle organ.And these researchs must be based on a good hair follicle culture model foundation.A desirable culture dish for hair follicle culture can be hair follicle culture model and provides support, and the mechanism of growing of also more in depth studying hair follicle for us provides outstanding condition.At present, hair follicle culture mainly contains two kinds of modes: a kind of is the cultivation of hairiness skin graft, and this training method can not realize the analyzing in detail of carrying out to single hair follicle; Another kind is by mechanical separation, to obtain several single hair follicles to put into the culture dish cultivation that dissociates, but is free in hair follicle in the nutrient solution cluster that easily sticks together, and this training method is also unfavorable for the analysis and research to single hair follicle.
summary of the invention
The utility model provide a kind of overcome prior art deficiency, simple in structure, be easy to make, easy to use, particularly can be used for a kind of device for hair follicle culture of single hair follicle culture.
A kind of culture apparatus for hair follicle culture of the present invention, by culture dish, culture dish lid, formed, the basal surface of culture dish is coated with by IV collagen type, in culture dish apart from the coated face of IV collagen type 3 to 5 millimeters be provided with the hair follicle that mesh is the cell screen cloth formation of 100 μ m and carry net.
A kind of culture apparatus for hair follicle culture of the present utility model can be to carry under net and be provided with the leg of 3 to 5 millimeters at hair follicle, utilizes this leg that hair follicle is carried to net and is arranged at apart from bottom surface 3 to 5 millimeters.
A kind of culture apparatus for hair follicle culture of the present utility model can be also the projection that place, 3 to 5 millimeters of span bottom surfaces is provided with location in culture dish side, and hair follicle carries net and is arranged on projection.
The culture dish basal surface of culture apparatus of the present utility model is coated through IV collagen type, can make hair follicle root cell paste albumen growth coated on diapire, and in culture dish, hair follicle carries net and single hair follicle can be isolated, and realizes single hair follicle culture research.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of a kind of culture apparatus for hair follicle culture of the utility model.
Fig. 2 is the structural representation of a kind of each isolation of components of culture dish for hair follicle culture of the utility model shown in Fig. 1.
In figure, 1---culture dish, 2---culture dish lid, 3---hair follicle carries net, and 4---be coated in the IV collagen type on culture dish basal surface.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearer, below in conjunction with accompanying drawing, embodiment of the present utility model is described in detail.
As depicted in figs. 1 and 2, device of the present utility model comprises that culture dish body, culture dish cover plate and hair follicle carry net.Wherein: culture dish lid 2 is for being placed on culture dish 1, for cultured object provides a suitable growing environment; On the bottom surface of culture dish 1, be coated with IV collagen type 4, for the indispensable conditions such as the needed nutrition of hair follicle growth are provided; On IV collagen type 4 upper surfaces, be provided with the hair follicle that cell screen cloth forms and carry net 3, the mesh size of cell screen cloth is 100 μ m.
In the utility model, can carry the leg that net arranges 3 to 5 millimeters for 3 times at hair follicle, hair follicle is carried between net and coated albumen and leave certain gap, or also can be in culture dish side place, 3 to 5 millimeters of span bottom surfaces be provided with the projection of location, and hair follicle is carried to net, be arranged on projection, capsule carried between net and coated albumen and leave certain gap.
The utility model preparation can first configure 0.5M acetic acid, and 0.22 μ m filtration sterilization is standby.1 ㎎ IV Collagen Type VI is added to 1ml 5M acetic acid, be prepared into 1mg/ml storage liquid, 20 ℃ of ﹣ save backup.
When specifically preparing the utility model, by storage liquid dilution, be 0.1mg/ml, with aseptic spreading rod, evenly coat common Tissue Culture Dish bottom, reduce as far as possible consumption, and it is even and thin just to make coating try one's best.After coating, be placed in 37 ℃ of incubators approximately 1 ~ 2h dry.Hair follicle carries net and is placed on the culture dish middle part through the coated bottom surface of IV Collagen Type VI through cleaning and sterilizing.
The culture dish adopting in embodiment of the present utility model is 35mm Tissue Culture Dish, cell screen cloth diameter 35 ㎜ used, the aperture 100 μ m of screen cloth.
The utility model is before use with first rinsing 3 times with PBS.With PBS, rinse 3 times,
While using the utility model, first with PBS, rinse 3 times, carry out hair follicle collection of specimens, the hair follicle being collected is cultivated to pre-treatment.Get single hair follicle and insert respectively in the single mesh that carries net, add nutrient solution and cultivate, the hair follicle phenomenon of cluster that sticks together while avoiding suspension culture.As after need obtain hair follicle root cell and only need add Digestive system and digest, take out residue hair follicle hair shaft and partly can obtain hair follicle root cell, for further research hair follicle biology is laid a good foundation.
Claims (3)
1. the culture apparatus for hair follicle culture, by culture dish, culture dish lid, formed, the basal surface that it is characterized in that culture dish is coated with by IV collagen type, in culture dish apart from the coated face of IV collagen type 3 to 5 millimeters be provided with the hair follicle that mesh is the cell screen cloth formation of 100 μ m and carry net.
2. a kind of culture apparatus for hair follicle culture according to claim 1, is characterized in that hair follicle carries under net, to be provided with the leg of 3 to 5 millimeters.
3. a kind of culture apparatus for hair follicle culture according to claim 1, is characterized in that place, interior 3 to 5 millimeters of the bottom surfaces of span of culture dish side is provided with the projection of location, and hair follicle carries net and is arranged on projection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420164275.4U CN203754727U (en) | 2014-04-07 | 2014-04-07 | Device for hair follicle culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420164275.4U CN203754727U (en) | 2014-04-07 | 2014-04-07 | Device for hair follicle culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203754727U true CN203754727U (en) | 2014-08-06 |
Family
ID=51250026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420164275.4U Expired - Fee Related CN203754727U (en) | 2014-04-07 | 2014-04-07 | Device for hair follicle culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203754727U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109182082A (en) * | 2018-08-29 | 2019-01-11 | 南京新生医疗科技有限公司 | hair follicles adipose tissue separating plate |
-
2014
- 2014-04-07 CN CN201420164275.4U patent/CN203754727U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109182082A (en) * | 2018-08-29 | 2019-01-11 | 南京新生医疗科技有限公司 | hair follicles adipose tissue separating plate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140806 Termination date: 20150407 |
|
| EXPY | Termination of patent right or utility model |