CN205917283U - A culture dish that is used for ventilative ceiling of ripe adipocyte to cultivate - Google Patents
A culture dish that is used for ventilative ceiling of ripe adipocyte to cultivate Download PDFInfo
- Publication number
- CN205917283U CN205917283U CN201620649708.4U CN201620649708U CN205917283U CN 205917283 U CN205917283 U CN 205917283U CN 201620649708 U CN201620649708 U CN 201620649708U CN 205917283 U CN205917283 U CN 205917283U
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- CN
- China
- Prior art keywords
- culture dish
- tripodia
- ceiling
- culture
- cell
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model discloses a culture dish that is used for ventilative ceiling of ripe adipocyte to cultivate, aluminum alloy's in including big culture dish and arranging big culture dish in tripodia ring carrier is provided with one deck PET film on the tripodia ring carrier. The utility model discloses a culture dish that is used for ventilative ceiling of ripe adipocyte to cultivate, add the ripe adipocyte suspension of 3ml in the big culture dish of 35mm, put into aseptic processing's the tripodia ring carrier 2 who is equipped with PET film 4 again, levitate cell was all attached in 4 downsides of PET film this moment, add the culture solution, make cell and the complete submergence of tripodia ring carrier 2 in the culture solution, put in carbon dioxide incubator, so both can concentrate the cell, guarantee abundant gas permeability again, do benefit to the good growth of cell greatly.
Description
Technical field
This utility model belongs to biological medicine experimental provision technical field, is related to a kind of culture dish and in particular to a kind of use
Culture dish in mature fat cell ventilative ceiling culture.
Background technology
Mature fat cell is serving not only as the storage unit of energy matter, and is actively adjusted by discharging active factorses
Organism metabolism under Different Nutrition state.Caused by adipose cell dysfunction such as lipid excess accumulation, obesity etc. has a strong impact on the mankind
Health.Both at home and abroad obese patient is increasing, and the function of further investigation adipose cell is for illustrating fat mechanism and searching
Effectively slimming medicine is very necessary.Therefore, cuituring adipocytes in vitro and then its function is carried out with further investigation is current state
Important research direction on border.Because mature fat cell contains triglyceride, density is little compared with aqueous solution, can swim in training during culture
Nutrient solution surface, therefore, the culture of mature fat cell all adopts ceiling method.The main commodity in use of existing ceiling culture method
The Nostoc commune Vanch bottle changed, thus bring inconvenience, it is unfavorable for that cell leads to if desired for after expending mass propgation liquid, closing bottleneck
Expend drug when gas, administration and collect sample inconvenience etc..Therefore, need one kind badly can be more particularly for cultivating ripe fat
The culture dish of cell.
Utility model content
The purpose of this utility model is to provide a kind of culture dish of ceiling culture of breathing freely for mature fat cell, this training
Foster ware reasonable in design, simple to operate, it is more suitable for cultivating mature fat cell.
This utility model be employed technical scheme comprise that, a kind of culture of ceiling culture of breathing freely for mature fat cell
Ware, including big culture dish and the tripodia ring support that is placed in the aluminum alloy material in big culture dish, tripodia ring support is arranged
There is one layer of pet thin film.
Feature of the present utility model also resides in,
Pet thin film is fixed on tripodia ring support by retainer ring and screw.
Big culture dish top is provided with top cover.
Tripodia ring support is positioned in the big culture dish of 35mm, the diameter 32mm of tripodia ring support, height 6mm.
The beneficial effects of the utility model are, a kind of culture dish of ceiling culture of breathing freely for mature fat cell, training
Foster ware entirety sterilization, after adding mature fat cell suspension, adipose cell is suspended under pet thin film, and culture fluid can
Tripodia ring support is simultaneously totally submerged by permeable membranes, thus ensureing mature fat cell complete wetting in culture fluid, simultaneously
Gas exchange can be carried out with the external world by pet film and bottom space, culture dish puts into CO2 gas incubator after covering ware lid.Should
Culture dish reasonable in design, simple to operate, can effectively cultivate mature fat cell.
Brief description
Fig. 1 is the structural representation of the culture dish that this utility model is used for mature fat cell ventilative ceiling culture.
In figure, 1. big culture dish, 2. tripodia ring support, 3. retainer ring, 4.pet thin film, 5. top cover.
Specific embodiment
With reference to the accompanying drawings and detailed description this utility model is described in detail.
This utility model is used for the culture dish of mature fat cell ventilative ceiling culture, and structure is as shown in figure 1, include big
Culture dish 1 and the tripodia ring support 2 being placed in the aluminum alloy material in big culture dish 1, tripodia ring support 2 is provided with one layer
Pet thin film 4.
Pet thin film 4 is fixed on tripodia ring support 2 by retainer ring 3 and screw.
Big culture dish 1 top is provided with top cover 5.
Tripodia ring support 2 is positioned in the big culture dish of 35mm 1, the diameter 32mm of tripodia ring support 2, height 6mm.Can
Make cell attachment to greatest extent on the downside of thin film, and do not affect the use of top cover 5.Tripodia ring support 2 adopts aluminium alloy material
Matter, can stably stand on big culture dish 1 bottom；Antirust and can autoclaving, reusable thus saving great amount of cost.
Using pet thin film 4, autoclaving and low cost can be tolerated；Pet thin film 4 has preferable light transmission, is easy to micro-
Cell state below Microscopic observation thin film.
This utility model is used for the culture dish of mature fat cell ventilative ceiling culture, when using:
Add 3ml mature fat cell suspension in the big culture dish of 35mm 1, place into the thin equipped with pet of aseptic process
The tripodia ring support 2 of film 4, the cell now floating all is attached on the downside of pet thin film 4, adds culture fluid so that cell and three
Sufficient ring support 2 is completely submerged in culture fluid, is put in CO2 gas incubator, so both can put together cell, protects again
Demonstrate,prove sufficient breathability, be significantly beneficial to the good growth of cell.
Claims (4)
1. a kind of for mature fat cell breathe freely ceiling culture culture dish it is characterised in that include big culture dish (1) and
It is placed in the tripodia ring support (2) of the aluminum alloy material in big culture dish (1), described tripodia ring support (2) is provided with one
Layer pet thin film (4).
2. according to claim 1 for mature fat cell breathe freely ceiling culture culture dish it is characterised in that institute
State pet thin film (4) to be fixed on tripodia ring support (2) by retainer ring (3) and screw.
3. the culture dish of ceiling culture of breathing freely for mature fat cell according to claim 1 and 2, its feature exists
In described big culture dish (1) top is provided with top cover (5).
4. according to claim 1 for mature fat cell breathe freely ceiling culture culture dish it is characterised in that institute
State tripodia ring support (2) to be positioned in the big culture dish of 35mm (1), the diameter 32mm of tripodia ring support (2), height 6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620649708.4U CN205917283U (en) | 2016-06-27 | 2016-06-27 | A culture dish that is used for ventilative ceiling of ripe adipocyte to cultivate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620649708.4U CN205917283U (en) | 2016-06-27 | 2016-06-27 | A culture dish that is used for ventilative ceiling of ripe adipocyte to cultivate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205917283U true CN205917283U (en) | 2017-02-01 |
Family
ID=57874638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620649708.4U Expired - Fee Related CN205917283U (en) | 2016-06-27 | 2016-06-27 | A culture dish that is used for ventilative ceiling of ripe adipocyte to cultivate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205917283U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345205A (en) * | 2017-09-06 | 2017-11-14 | 阜阳师范学院 | A kind of mature fat cell dedifferentes the device and method of culture |
| CN110295110A (en) * | 2019-06-26 | 2019-10-01 | 吴威 | A kind of equipment increasing body fat cell survival quantity |
-
2016
- 2016-06-27 CN CN201620649708.4U patent/CN205917283U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345205A (en) * | 2017-09-06 | 2017-11-14 | 阜阳师范学院 | A kind of mature fat cell dedifferentes the device and method of culture |
| CN110295110A (en) * | 2019-06-26 | 2019-10-01 | 吴威 | A kind of equipment increasing body fat cell survival quantity |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170201 Termination date: 20200627 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |