CN103805500A - Offing microalgae cultivation device and cultivation method - Google Patents

Offing microalgae cultivation device and cultivation method Download PDF

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CN103805500A
CN103805500A CN201410059320.4A CN201410059320A CN103805500A CN 103805500 A CN103805500 A CN 103805500A CN 201410059320 A CN201410059320 A CN 201410059320A CN 103805500 A CN103805500 A CN 103805500A
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incubator
stainless steel
micro
liquid
microalgae
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陈春辉
杨宗鑫
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Hangzhou Xinwei Low Carbon Technology R & D Co Ltd
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    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract

The invention discloses an offing microalgae cultivation device and an offing microalgae cultivation method. The offing microalgae cultivation device comprises an incubator sleeved on standing posts through main stainless steel lantern-rings, wherein the upper edges of the two sides of the incubator are connected with operating paths, which are sleeved on the standing posts through main stainless steel lantern-rings, through nylon ropes passing through multiple assistant stainless steel lantern-rings; the incubator and the operating paths float upwards and downwards together in the offing; the incubator is also covered with a transparent film, and at least one carbon dioxide supplementing pipeline and at least one microalgae cultivation liquid import and microalgae liquid collecting interface are arranged in the incubator and puncture the transparent plastic film from the upper surface of the film. The microalgae cultivation method comprises the steps of importing microalgae cultivation liquid into the incubator, and feeding carbon dioxide; under a buoyancy force provided by the operating paths, uniformly mixing carbon dioxide and nutritive salt through the turbulence of sea waves, by illuminating, beginning to cultivate microalgae, after the cultivation is completed, closing the carbon dioxide supplementing pipeline, and carrying out collecting and subsequent processing on the microalgae through the microalgae cultivation liquid import and microalgae liquid collecting interface.

Description

A kind of sea micro algae culturing device and cultural method
Technical field
What the present invention relates to is a kind of sea micro algae culturing device and cultural method, especially utilize a sea micro algae culturing device for bay, shallow sea, sea and beach, belong to bioenergy, aquaculture, marine organism health care product, marine organisms medicine and other fields.
Technical background
Along with the rise of micro-algae heat, to the research of micro-algae industrial chain further deeply, the research and development of micro algae culturing device are one of main contents wherein to people.At present, the research of micro algae culturing device mainly concentrates on the various bio-reactors of land, and as raceway pond, column, tubulose, tabular bioreactor, relate to micro algae culturing device on sea few, large scale culturing device or even blank on sea.In order to cultivate across the sea micro-algae, people have designed transparent plastic film bag and tubular micro algae culturing device, and transparent plastic film bag is difficult for fixing, algae liquid reclaims and supplements carbonic acid gas, and being not suitable for large scale culturing; Tubular micro algae culturing device price is higher, cultivates cost high for extensive micro-algae; And China per capita can be few with cultivated area, government does not allow the resource that occupies cultivated land.
Summary of the invention
The object of the invention is to overcome the deficiency that prior art exists, and provide a kind of, both can not occupy cultivated land, can meet again sea micro algae culturing device and the cultural method of micro-algae large scale culturing; It can solve the problem of the extensive micro-algae cultivation of shallow sea, sea, bay and beach aspect, and utilize shallow sea, bay, beach large-scale culturing micro-algae to meet the fundamental realities of the country of China, for research, the exploitation of micro-algae bioenergy and micro-algae Medicines and Health Product provide raw material.
To achieve these goals, a kind of sea provided by the invention micro algae culturing device, it comprises that one by main stainless steel sleeve ring set incubator on column, it is also the operation road on column by main stainless steel sleeve ring set that the both sides upper limb of described incubator passes by nylon rope that multiple secondary stainless steel collars are connected with, and by described operation road provide buoyancy together with incubator floating across the sea; On incubator, be also coated with transparent mulch film, at least one carbonic acid gas supplements pipeline and the importing of at least one micro algae culturing liquid and algae liquid collection interface and is positioned in incubator and from passing transparent plastic film above.
Described incubator is square, is all connected with the main stainless steel collar movable up and down on eight angles of incubator, and by this main stainless steel collar, incubator is enclosed within on four vertically arranged four root posts in angle; Described operation road is positioned at incubator one side and is provided with the securing line fixedly being formed by nylon rope.
Described incubator has the secondary stainless steel collar to inlay at the upper limb of operation both sides, road between 0.5-1m, in the secondary stainless steel collar, after interspersed nylon rope, incubator and operation road is fixed together, and to cubical incubator sizing; Incubator have multiple and by and connect, series connection or mixed connection mode are formed by connecting built-up type micro algae culturing device.
Described operation road is formed by connecting by foam plastic reservoir, and its height is at 0.4-1.5m, and width is at 0.6-1.5m, and bottom and top all can exceed incubator, and concrete numerical value is determined according to required height and the buoyancy that exceeds the water surface of incubator.
Described column height is greater than the degree of depth of time of high water water body, generally should exceed water surface 1m left and right, and the degree of depth of incubator is 40-60cm, and medium depth is 25-30cm.
Described incubator is made by the thick water conservancy project cloth of 0.5-1.5mm, height 0.4-0.6m, length 5-40m, width 2-16m; On incubator, have thickness be 0.1-1mm, four jiaos have the stainless steel collar, for clear PE or the PVC mulch film of dustproof, rainproof and anti-other foreign material.
On described mulch film, be provided with two collars that are respectively used to through micro algae culturing liquid importing and algae liquid collection interface and the supplementary pipeline of carbonic acid gas.
One utilizes above-mentioned sea micro algae culturing device to carry out microalgae culture method, and described microalgae culture method comprises: connect micro algae culturing liquid importing and algae liquid and collect interface and the supplementary pipeline of carbonic acid gas, micro algae culturing liquid is imported to incubator, pass into carbonic acid gas; Under the buoyancy that provides of operation road, the flood tide incubator that still ebbs tide all will swim on the water surface; By jolting of wave, carbonic acid gas and nutritive salt are mixed, through illumination, micro-algae is cultivated and starts, and cultivates to finish rear closing carbon dioxide and supplement pipeline, is imported and algae liquid is collected interface and carried out micro-algae collection and subsequent disposal by micro algae culturing liquid.
It is simple in structure, with low cost, easy to operate that the present invention has, and can meet the features such as micro-algae cultivation of bay, sea, shallow sea and beach.
Accompanying drawing explanation
Fig. 1 is the structural representation of bay of the present invention micro algae culturing device.
Label in figure is: 1-operates road, and 2-carbonic acid gas supplements pipeline, the secondary stainless steel collar of 3-, and 4-micro algae culturing liquid imports and algae liquid is collected interface, 5-column, 6-mulch film, 7-incubator, the main stainless steel collar of 8-, 9-nylon rope, 10-securing line.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be described in detail: shown in Fig. 1, sea of the present invention micro algae culturing device, it comprises that one is enclosed within incubator 7 on column 5 by the main stainless steel collar 8, it is to be also enclosed within the operation road 1 on column 5 by the main stainless steel collar 8 that the both sides upper limb of described incubator 7 is connected with through multiple secondary stainless steel collars 3 by nylon rope 9, and provides buoyancy to float across the sea together with incubator 7 by described operation road 1; On incubator 7, be also coated with transparent mulch film 6, at least one carbonic acid gas supplements pipeline 2 and the importing of at least one micro algae culturing liquid and algae liquid collection interface 4 and is positioned in incubator 7 and from passing transparent plastic film 6 above.
Described incubator 7 is square, is all connected with the main stainless steel collar 8 movable up and down on eight angles of incubator, and by this main stainless steel collar 8, incubator 7 is enclosed within on four vertically arranged four root posts 5 in angle; Described operation road 1 is positioned at incubator one side and is provided with the securing line 10 fixedly being formed by nylon rope.
Described incubator 7 has the secondary stainless steel collar 3 to inlay at the upper limb of operation 1 both sides, road between 0.5-1m, after interspersed nylon rope 9, incubator 7 and operation road 1 is fixed together, and cubical incubator 7 is shaped in the secondary stainless steel collar 3; Incubator 7 have multiple and by and connect, series connection or mixed connection mode are formed by connecting built-up type micro algae culturing device.
Described operation road 1 is formed by connecting by foam plastic reservoir, and its height is at 0.4-1.5m, and width is at 0.6-1.5m, and bottom and top all can exceed incubator, and concrete numerical value is determined according to required height and the buoyancy that exceeds the water surface of incubator.
Described column height is greater than the degree of depth of time of high water water body, generally should exceed water surface 1m left and right, and the degree of depth of incubator is 40-60cm, and medium depth is 25-30cm.
Described incubator 7 is made by the thick water conservancy project cloth of 0.5-1.5mm, height 0.4-0.6m, length 5-40m, width 2-16m; On incubator 7, have thickness be 0.1-1mm, four jiaos have the stainless steel collar, for clear PE or the PVC mulch film of dustproof, rainproof and anti-other foreign material.
On described mulch film, be provided with two collars that are respectively used to through micro algae culturing liquid importing and algae liquid collection interface and the supplementary pipeline of carbonic acid gas.
One utilizes above-mentioned sea micro algae culturing device to carry out microalgae culture method, described microalgae culture method comprises the steps: to connect micro algae culturing liquid importing and algae liquid is collected interface and carbonic acid gas supplements pipeline, micro algae culturing liquid is imported to incubator, pass into carbonic acid gas; Under the buoyancy that provides of operation road, the flood tide incubator that still ebbs tide all will swim on the water surface; By jolting of wave, carbonic acid gas and nutritive salt are mixed, through illumination, micro-algae is cultivated and starts, and cultivates to finish rear closing carbon dioxide and supplement pipeline, is imported and algae liquid is collected interface and carried out micro-algae collection and subsequent disposal by micro algae culturing liquid.
Embodiment: the present invention mainly comprises:
One operation road, be formed by connecting by foam plastic reservoir, lead to bank, it is highly 0.4-1.5m, width is 0.6-1.5m, and bottom and top all can exceed incubator, and concrete numerical value is determined according to required height and the buoyancy that exceeds the water surface of incubator, it and incubator pass the stainless steel collar by nylon rope and fix, and provide buoyancy for the daily cultivation of micro-algae management and for incubator.
One incubator, is formed by water conservancy project cloth processing customization, and thickness is 0.5-1.5mm, height 0.4-0.6m, and length 5-40m, width 2-16m, cultivates for micro-algae.On its eight angles, there is the stainless steel collar, for incubator is enclosed within to column, can slide up and down; Its upper limb that is positioned at operation both sides, road respectively has row's stainless steel collar, fixes for wearing to look into nylon rope and operate road.
The transparent film that has PE or PVC to be made at described incubator top, i.e. mulch film 6, its thickness is 0.1-1mm, four jiaos of plastics films have stainless steel sleeve ring set on column, enter incubator for rainproof, dustproof and anti-other foreign material; The side that transparent film is positioned at operation road also has two collars, for importing by micro algae culturing liquid and algae liquid collection interface and the supplementary pipeline of carbonic acid gas.
Described incubator and operation road are by stainless steel sleeve ring set on column, and the height of column should exceed time of high water water depth 1m left and right.
Described operation road one side has securing line, is fixed on column, and the quantity of column can be according to actual needs magnitude setting, and mainly definite according to the firm degree of securing line, for cultivation, personnel grip, and guarantee operational safety.
Described culture apparatus can and connect, series winding or mixed connection, and the size of cultivating place according to micro-algae arranges the quantity of culture apparatus.
Embodiment 1: shown in Fig. 1, bay of the present invention micro algae culturing device, it comprises: an incubator 7, it is enclosed within on column 5 by the stainless steel collar 8, the both sides upper limb that incubator 7 is connected with operation road 1 has can be through the stainless steel collar 3 of nylon rope 9, on it, also have transparent film, mulch film 6 covers, carbonic acid gas supplements that pipeline 2 and micro algae culturing liquid import and algae liquid is collected interface 4 and can be passed transparent plastic film 6; An operation road 1, it and incubator 7 are all enclosed within on column 5, prevent culture apparatus waft from, operation road be positioned at the securing line 10 that incubator one side has nylon rope fixedly to form, for cultivation, personnel grip, and guarantee operational safety.
Described incubator 7 sizes are adjustable, and shape can be square or other shapes, mainly customizes according to practical situation; Operation road 1 can exceed incubator top and bottom, determines according to buoyancy situation; Carbonic acid gas supplements the thickness of pipeline 2 with whether branch can determine according to incubator area; Micro algae culturing liquid imports and algae liquid is collected the size of interface 4 and the width in operation road 1, highly can be determined according to the micro-algae cultivation of reality situation with buoyancy; The height of column 5 should exceed the 1m left and right of the time of high water depth of water, and the quantity of column 5 can be determined according to the firm degree of securing line; Incubator 7 is fixed together through the stainless steel collar 3 through nylon rope 9 with operation road 1, and they are all enclosed within on column 5 by the stainless steel collar 8, can on column 5, slide up and down, and provide buoyancy to swim on the water surface by operation road 1.
The method that the present invention utilizes described sea micro algae culturing device to carry out micro-algae cultivation, specific works process is: cultivation personnel are holding up securing line to operation road, connect micro algae culturing liquid importing and algae liquid and collect interface 4, import micro algae culturing liquid, in the time that the liquid level of incubator 7 reaches 30cm, stop importing nutrient solution, connect carbonic acid gas and supplement pipeline 2, pass into carbonic acid gas; Stir and mix carbonic acid gas and various nutritive salt through wave, under photosynthesis, micro-algae Fast Growth breeding; At the incoming tide, sea level rise, and operation road 1 provides buoyancy for incubator 7, the stainless steel collar 8 upward slidings, incubator is across the sea floating, and sea level declines at ebb tide, incubator is along with seawater declines, and incubator continues across the sea floating or provided support and be shelved on beach by operation road; When algae liquid arrives the concentration of collecting, closing carbon dioxide is supplemented interface 2, imports and algae liquid is collected interface 4 and collected algae liquid by micro algae culturing liquid, realizes bay, shallow sea, the beach cultivation of micro-algae.

Claims (8)

1. a sea micro algae culturing device, it comprises that one is enclosed within the upper incubator (7) of column (5) by the main stainless steel collar (8), it is to be also enclosed within the operation road (1) on column (5) by the main stainless steel collar (8) that the both sides upper limb that it is characterized in that described incubator (7) is connected with through multiple secondary stainless steel collars (3) by nylon rope (9), and provides buoyancy to float across the sea with incubator (7) together with by described operation road (1); On incubator (7), be also coated with transparent mulch film (6), at least one carbonic acid gas supplements pipeline (2) and the importing of at least one micro algae culturing liquid and algae liquid collection interface (4) and is positioned in incubator (7) and from passing transparent plastic film (6) above.
2. sea according to claim 1 micro algae culturing device, it is characterized in that described incubator (7) is for square, on eight angles of incubator, be all connected with the main stainless steel collar movable up and down (8), and by this main stainless steel collar (8), incubator (7) be enclosed within on four vertically arranged four root posts in angle (5); Described operation road (1) is positioned at incubator one side and is provided with the securing line (10) fixedly being formed by nylon rope.
3. sea according to claim 1 and 2 micro algae culturing device, the upper limb that incubator (7) described in it is characterized in that is positioned at operation both sides, road (1) has the secondary stainless steel collar (3) to inlay between 0.5-1m, in the secondary stainless steel collar (3), after interspersed nylon rope (9), incubator (7) and operation road (1) are fixed together, and cubical incubator (7) is shaped; Incubator (7) have multiple and by and connect, series connection or mixed connection mode are formed by connecting built-up type micro algae culturing device.
4. sea according to claim 3 micro algae culturing device, it is characterized in that described operation road (1) is formed by connecting by foam plastic reservoir, its height is at 0.4-1.5m, width is at 0.6-1.5m, bottom and top all can exceed incubator (7), and concrete numerical value is determined according to required height and the buoyancy that exceeds the water surface of incubator (7).
5. sea according to claim 1 and 2 micro algae culturing device, it is characterized in that described column (5) is highly greater than the degree of depth of time of high water water body, generally should exceed water surface 1m left and right, the degree of depth of incubator (7) is 40-60cm, and medium depth is 25-30cm.
6. sea according to claim 1 and 2 micro algae culturing device, is characterized in that described incubator (7) is made by the thick water conservancy project cloth of 0.5-1.5mm, height 0.4-0.6m, length 5-40m, width 2-16m; On incubator (7), have thickness be 0.1-1mm, four jiaos have the secondary stainless steel collar, for clear PE or the PVC mulch film (6) of dustproof, rainproof and anti-other foreign material.
7. sea according to claim 6 micro algae culturing device, is provided with two collars that are respectively used to through micro algae culturing liquid importing and algae liquid collection interface and the supplementary pipeline of carbonic acid gas on the mulch film (6) described in it is characterized in that.
8. one kind is utilized arbitrary described sea micro algae culturing device in claim 1-7 to carry out microalgae culture method, it is characterized in that described microalgae culture method comprises: connect micro algae culturing liquid importing and algae liquid and collect interface and the supplementary pipeline of carbonic acid gas, micro algae culturing liquid is imported to incubator, pass into carbonic acid gas; Under the buoyancy that provides of operation road, the flood tide incubator that still ebbs tide all will swim on the water surface; By jolting of wave, carbonic acid gas and nutritive salt are mixed, through illumination, micro-algae is cultivated and starts, and cultivates to finish rear closing carbon dioxide and supplement pipeline, is imported and algae liquid is collected interface and carried out micro-algae collection and subsequent disposal by micro algae culturing liquid.
CN201410059320.4A 2014-02-21 2014-02-21 Offing microalgae cultivation device and cultivation method Pending CN103805500A (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104593224A (en) * 2015-02-15 2015-05-06 润科生物工程(福建)有限公司 Special photo-biological reactor system for driving microalgae cultivation by multi-stage potential energy differences
CN107384801A (en) * 2017-08-21 2017-11-24 李彤 Closing biological membrane type cultural method for industrialized production microalgae
CN107460129A (en) * 2017-08-21 2017-12-12 李彤 The industrialization microalgae culture method that biomass separates with nutrient solution
CN107475069A (en) * 2017-08-15 2017-12-15 大连理工大学 Floatation type microalgae culture system and microalgae culture method
CN109321468A (en) * 2018-12-12 2019-02-12 无棣鲁东大学海洋研究院 The method and both culturing microalgae system of cultivating microalgae in a kind of region of ocean shoal
CN109439549A (en) * 2018-12-22 2019-03-08 无棣鲁东大学海洋研究院 The method and both culturing microalgae system of cultivating microalgae in a kind of ocean deepwater area
CN110343605A (en) * 2019-08-26 2019-10-18 山东省分析测试中心 A kind of biological floating bed bioreactor and microdisk electrode and water body purification method
CN112877179A (en) * 2021-03-12 2021-06-01 大连理工大学 Wave amplification device for culturing microalgae
CN115404168A (en) * 2022-11-01 2022-11-29 山东滨州八匹马塑料化纤制品有限公司 Marine microalgae cultivation method

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Publication number Priority date Publication date Assignee Title
CN1961660A (en) * 2006-12-18 2007-05-16 卢振琦 Float type biological culture pond
CN201334475Y (en) * 2008-12-09 2009-10-28 朱洪 Device for water cultivation engineering
CN102307985A (en) * 2008-12-03 2012-01-04 仁荷大学校产学协力团 Photobioreactor for a large-scale marine microalgal culture using half-transmitting film
CN102517218A (en) * 2011-12-28 2012-06-27 广东海洋大学 Method for culturing microalgae on sea surface

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1961660A (en) * 2006-12-18 2007-05-16 卢振琦 Float type biological culture pond
CN102307985A (en) * 2008-12-03 2012-01-04 仁荷大学校产学协力团 Photobioreactor for a large-scale marine microalgal culture using half-transmitting film
CN201334475Y (en) * 2008-12-09 2009-10-28 朱洪 Device for water cultivation engineering
CN102517218A (en) * 2011-12-28 2012-06-27 广东海洋大学 Method for culturing microalgae on sea surface

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104593224A (en) * 2015-02-15 2015-05-06 润科生物工程(福建)有限公司 Special photo-biological reactor system for driving microalgae cultivation by multi-stage potential energy differences
CN107475069A (en) * 2017-08-15 2017-12-15 大连理工大学 Floatation type microalgae culture system and microalgae culture method
CN107384801A (en) * 2017-08-21 2017-11-24 李彤 Closing biological membrane type cultural method for industrialized production microalgae
CN107460129A (en) * 2017-08-21 2017-12-12 李彤 The industrialization microalgae culture method that biomass separates with nutrient solution
CN109321468A (en) * 2018-12-12 2019-02-12 无棣鲁东大学海洋研究院 The method and both culturing microalgae system of cultivating microalgae in a kind of region of ocean shoal
CN109439549A (en) * 2018-12-22 2019-03-08 无棣鲁东大学海洋研究院 The method and both culturing microalgae system of cultivating microalgae in a kind of ocean deepwater area
CN110343605A (en) * 2019-08-26 2019-10-18 山东省分析测试中心 A kind of biological floating bed bioreactor and microdisk electrode and water body purification method
CN112877179A (en) * 2021-03-12 2021-06-01 大连理工大学 Wave amplification device for culturing microalgae
CN115404168A (en) * 2022-11-01 2022-11-29 山东滨州八匹马塑料化纤制品有限公司 Marine microalgae cultivation method

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Application publication date: 20140521