CN109321468A - The method and both culturing microalgae system of cultivating microalgae in a kind of region of ocean shoal - Google Patents
The method and both culturing microalgae system of cultivating microalgae in a kind of region of ocean shoal Download PDFInfo
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- CN109321468A CN109321468A CN201811514606.1A CN201811514606A CN109321468A CN 109321468 A CN109321468 A CN 109321468A CN 201811514606 A CN201811514606 A CN 201811514606A CN 109321468 A CN109321468 A CN 109321468A
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- 238000012258 culturing Methods 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 241000195493 Cryptophyta Species 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims description 4
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 239000013535 sea water Substances 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000003760 hair shine Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 2
- 229940033663 thimerosal Drugs 0.000 description 2
- 230000005791 algae growth Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The invention discloses the methods and its both culturing microalgae system of cultivating microalgae in a kind of region of ocean shoal, its main feature is that, several seabed spud piles are set in the region of ocean shoal and surround culture zone, fixed rope and fixed rope waterborne in water are connected between spud pile；Several both culturing microalgae polybags are connected in water between fixed rope and fixed rope waterborne；Exhaust pipe is arranged in the upper side of both culturing microalgae polybag, and the exhaust pipe is made to be fixed up opening by connecting fixed rope waterborne；Both culturing microalgae polybag is additionally provided with expects pipe and passes in and out for material；Both culturing microalgae polybag is connected on gas-guide tube by air inlet pipe, and gas-guide tube is connected with blower；The method and both culturing microalgae system of cultivating microalgae in a kind of region of ocean shoal are provided, further expand both culturing microalgae coverage of water, realize the marine environment cultivation of microalgae, mitigate influence of the environment to algae pollution, simultaneously, microalgae is kept to be independent of each other in the breeding process, it is in stable condition, improve algae culturing industry economic benefit.
Description
Technical field
The present invention relates to a kind of methods of cultivating microalgae, and in particular in a kind of region of ocean shoal the side of cultivating microalgae
Method.The invention further relates to the both culturing microalgae systems for implementing the method for cultivating microalgae in the region of the ocean shoal.
Background technique
Microalgae is that one kind is widely distributed in water body, and the high autophyte of full of nutrition, photosynthetic availability can pass through it
Efficient photosynthesis absorbs CO using luminous energy2And H2It is organic to convert them into carbohydrate, protein, fat etc. by O
Object, and release O2.Polysaccharide, protein, grease, the pigment etc. that cell metabolism generates, make its food, medicine, genetic engineering,
The fields such as liquid fuel have good development prospect.
Microalgae it is many kinds of and it is individual be generally less than 2mm.Currently, both culturing microalgae mostly uses greatly the open pond in land to be supported
It grows.But the breeding way has the disadvantage in that one, due to needing sufficient illumination in microalga cultivation process, can not pass through increasing
Breeding water body is opened up in the measures such as plus depth, so taking up a large area, farm is limited, it is difficult to realize that large area, scale are supported
It grows.Two, terrestrial environment is poor, easily makes cultivated microalgae contaminated；Three, it is with high salt, high-alkali to be adapted only to fraction for the breeding way
The microalgae of equal extreme environments existence, the type of cultivating microalgae are limited.
It is " a kind of to shine cultivating microalgae in salt pond that this forward direction China Intellectual Property Office of the applicant has submitted application for a patent for invention
Field of microalgae cultivation is extended to seawater from land aquaculture pond and shines salt pond by method and both culturing microalgae system ", so that part solves
The above problem.But the usable floor area that seawater shines salt pond nevertheless suffers from larger limitation, and promotion prospect and economic benefit still cannot
It is satisfactory.
Summary of the invention
The technical problem to be solved by the invention is to provide the methods and microalgae of cultivating microalgae in a kind of region of ocean shoal
Cultivating system further expands both culturing microalgae coverage of water, realizes the marine environment cultivation of microalgae, mitigates dirt of the environment to algae
Dye, meanwhile, keep microalgae to be independent of each other in the breeding process, it is in stable condition, improve algae culturing industry economic benefit.
In order to solve this technical problem, the invention adopts the following technical scheme:
The method of cultivating microalgae in a kind of region of ocean shoal, which is characterized in that several seabeds are set in the region of ocean shoal
Spud pile surrounds culture zone, and fixed rope and fixed rope waterborne in water are connected between spud pile；Fixed rope and fixed rope waterborne in water
Between connect several both culturing microalgae polybags；Exhaust pipe is arranged in the upper side of both culturing microalgae polybag, and keeps the exhaust pipe logical
It crosses and connects fixed rope waterborne and be fixed up opening；Both culturing microalgae polybag is additionally provided with expects pipe and passes in and out for material；Both culturing microalgae
Polybag is connected on gas-guide tube by air inlet pipe, and gas-guide tube is connected with blower.
For implementing the both culturing microalgae system of the method for cultivating microalgae in ocean shoal region described in claim 1,
It is characterized in that it includes four vertex spud piles that seabed is fixed in bottom end, four vertex spud piles surround in ocean shoal region
Two relative sides of rectangle culture zone, the rectangle culture zone are respectively equipped with several side spud piles, the side of every side
Spud pile is equidistantly arranged between the spud pile of the side vertex Liang Gen and bottom end is both secured to seabed；The side of two relative sides
Side spud pile is arranged correspondingly, is fixedly connected between each opposite side spud pile solid in the water that one is located at below the water surface
Fixed rope, the fixed rope waterborne being also fixedly connected with right above the fixed rope that is in water；Fixed rope and fixation waterborne in water
At least one longitudinal direction gas-guide tube for being provided with several lateral gas-guide tubes between rope and being communicated with each item transverse direction gas-guide tube；It is described
System further includes several both culturing microalgae polybags being in water between fixed rope and fixed rope waterborne；Each both culturing microalgae
The downside of polybag is all connected with fixed rope in water；Both culturing microalgae polybag is connected on lateral gas-guide tube by air inlet pipe；
The upper side of both culturing microalgae polybag is also connected with exhaust pipe, and the inner end of exhaust pipe is communicated with both culturing microalgae polybag inner cavity, row
Tracheae connects on fixed rope on the water and ensures that outer end opening is upward by fixed rope waterborne；Both culturing microalgae polybag is also fixed to be connected
It is connected to disengaging of the expects pipe for material；The system also includes operating console, the operating console is solid by seabed spud pile
Dingan County is loaded on the side of rectangle culture zone, is equipped with blower on operating console；The longitudinal direction gas-guide tube connects wind by air valve
Machine；The vertex spud pile also passes through rope and connects the adjacent lateral gas-guide tube both ends of the vertex spud pile, passes through tension rope
Rope plays fixed function to gas-guide tube.
Preferably, each lateral gas-guide tube is isometric and is parallel to the long side in rectangle ocean shoal region, longitudinal air guide
Pipe overlaps through each lateral gas-guide tube, the central axes of longitudinal gas-guide tube and each lateral gas-guide tube.
Preferably, the ocean shoal region is the shoal equipped with wave-blocking dam.
Preferably, the inner end of expects pipe is located at both culturing microalgae polybag inner cavity, and outer end is equipped with sealing-plug.
Preferably, the depth of water in ocean shoal region where rectangle culture zone is no more than one meter.
The positive effect of the present invention is:
One, the present invention fixes Yu Haiyang shoal by the way that microalgae to be placed in both culturing microalgae polybag, and by both culturing microalgae polybag
In region, it is effectively utilized ocean shoal region area, both culturing microalgae coverage of water is greatly improved；And it can take on the spot
Material, direct extracting seawater cultivating microalgae, more economical convenient, saving labour, while ensure that the stabilization of micro algae growth environment,
Effectively prevent pollution of the terrestrial environment to microalgae.Mode through the invention can be realized large area artificial breeding microalgae, reach
To the purpose for increasing culture fishery economic benefit.It is cultivated by the way that different microalgae to be placed in different both culturing microalgae polybags,
It can be achieved while cultivating a variety of microalgaes, the cultivation type of microalgae is no longer limited.
Two, both culturing microalgae polybag is connected with fixed rope, fixed rope waterborne and lateral gas-guide tube in water respectively, solid in water
Fixed rope and fixed rope both ends waterborne are connected on spud pile, and lateral gas-guide tube is connected on spud pile by rope.Exhaust pipe connects
Connecing on fixed rope on the water and by fixed rope waterborne ensures that outer end opening is upward.Present system ensures both culturing microalgae polybag
Position relative securement in the seawater, it is small by Lidar Equation, it will not be interfered with each other between each both culturing microalgae polybag.
Detailed description of the invention
Fig. 1 is both culturing microalgae system structure diagram of the invention；
Fig. 2 is both culturing microalgae plastic bag structure schematic top plan view of the invention；
Fig. 3 is both culturing microalgae plastic bag structure left view schematic diagram of the invention.
Specific embodiment
The present invention is further illustrated with reference to the accompanying drawings and examples.
Cultural method of the invention are as follows: several seabed spud piles are set in the region of ocean shoal and surround culture zone, it is fixed
Fixed rope and fixed rope waterborne in water are connected between stake；Several both culturing microalgaes are connected in water between fixed rope and fixed rope waterborne
Polybag；Exhaust pipe is arranged in the upper side of both culturing microalgae polybag, and make the exhaust pipe by connect fixed rope waterborne it is fixed to
Upper opening；Both culturing microalgae polybag is additionally provided with expects pipe and passes in and out for material；Both culturing microalgae polybag is connected to by air inlet pipe
On gas-guide tube, gas-guide tube is connected with blower.
Both culturing microalgae system embodiment of the invention is as follows:
As shown in Figure 1, the embodiment of present system include: bottom end be fixed on seabed four vertex spud piles 3 it is shallow in ocean
Beach region surrounds rectangle culture zone 2, and two relative sides (preferably two long sides) of the rectangle culture zone 2 are if be respectively equipped with
A dry side spud pile 15, the side spud pile 15 of every side be equidistantly arranged between the side vertex Liang Gen spud pile 3 and
Bottom end is both secured to seabed.The side spud pile 15 of two relative sides is arranged correspondingly, each opposite side spud pile 15
Between be fixedly connected with one be located at the water surface below water in fixed rope 16, be also fixedly connected with the fixed rope 16 that is in water
The fixed rope waterborne 10 of surface, such as Fig. 3.Several transverse directions are provided between fixed rope 16 and fixed rope waterborne 10 in water to lead
Tracheae 1 and at least one longitudinal direction gas-guide tube 5 communicated with each item transverse direction gas-guide tube 1.
The embodiment of present system further includes that several that be in water between fixed rope 16 and fixed rope waterborne 10 are micro-
Algae cultivates polybag 6.The downside of each both culturing microalgae polybag 6 is connected with fixed rope 16 in water, guarantees both culturing microalgae
Polybag 6 is fixed movable in a certain range, keeps the stability of its internal microalgae, guarantees between both culturing microalgae polybag 6 mutually
Do not interfere, does not influence the cultivation situation of microalgae in each both culturing microalgae polybag 6.
In conjunction with Fig. 1, Fig. 2 and Fig. 3, both culturing microalgae polybag 6 is connected on lateral gas-guide tube 1 by air inlet pipe 7, air inlet pipe
7 effect includes: first, for being connected to both culturing microalgae polybag 6 and lateral gas-guide tube 1, and second, by both culturing microalgae polybag 6
It is connected on lateral gas-guide tube 1 and excessive excursions, therefore air inlet pipe 7 and both culturing microalgae polybag is avoided by the fixed position of the latter
It is needed between 6 fastened to each other in order to which entirety is put into water and recycled in its entirety.The upper side of both culturing microalgae polybag 6 is also connected with
The inner end of exhaust pipe 9, exhaust pipe 9 is communicated with 6 inner cavity of both culturing microalgae polybag, and exhaust pipe 9 connects on fixed rope 10 on the water simultaneously
Ensure that outer end opening is upward by fixed rope 10 waterborne, prevent from falling because of 9 side of exhaust pipe, liquid enters in exhaust pipe 9, guarantees microalgae
Gas in cultivation polybag 6 is smoothly discharged.Both culturing microalgae polybag 6 is also fixedly connected with expects pipe 8 for algae, algae solution, sea
The disengaging of water, nutrient solution and thimerosal etc., the inner end of expects pipe 8 are located at 6 inner cavity of both culturing microalgae polybag, and outer end is equipped with sealing-plug
14。
The embodiment of present system further includes operating console 13, which is fixed by seabed spud pile
It is installed on the side of rectangle culture zone 2.Blower 12 is installed on operating console 13.The longitudinal direction gas-guide tube 5 passes through air valve 11
Connect blower 12.
The vertex spud pile 3 also connects adjacent 1 both ends of lateral gas-guide tube of the vertex spud pile 3 by rope 4, leads to
It crosses tension rope 4 and fixed function is played to gas-guide tube.
Each lateral gas-guide tube 1 is isometric and is parallel to the horizontal edge in the shoal equipped with wave-blocking dam, and longitudinal gas-guide tube 5 runs through
The central axes of each transverse direction gas-guide tube 1, longitudinal gas-guide tube 5 and each lateral gas-guide tube 1 overlap.
The depth of water in 2 place ocean shoal region of rectangle culture zone is usually no more than one meter, and in 2 side of rectangle culture zone
Face is equipped with wave-blocking dam.
Algae, seawater, nutrient solution and thimerosal etc. are filled in both culturing microalgae polybag 6 respectively.Open blower 12 and air valve
11, into both culturing microalgae polybag 6 in be passed through air, provide carbon dioxide for the photosynthesis of microalgae.Extra air and light
The oxygen that cooperation generates is discharged upwards by exhaust pipe 9.Sealing-plug 14 is opened, using machinery suction or manual operations, is led to
Charge tube 8 periodically extraction section algae solution and fills into the seawater and nutrient solution of respective volume from both culturing microalgae polybag 6.
Claims (6)
1. the method for cultivating microalgae in a kind of region of ocean shoal, it is characterised in that: several seas are arranged in the region of ocean shoal
Bottom spud pile surrounds culture zone, and fixed rope and fixed rope waterborne in water are connected between spud pile；Fixed rope and fixation waterborne in water
Several both culturing microalgae polybags are connected between rope；Exhaust pipe is arranged in the upper side of both culturing microalgae polybag, and makes the exhaust pipe
Opening is fixed up by connecting fixed rope waterborne；Both culturing microalgae polybag is additionally provided with expects pipe and passes in and out for material；Microalgae is supported
It grows polybag to be connected on gas-guide tube by air inlet pipe, gas-guide tube is connected with blower.
2. the both culturing microalgae system for implementing the method for cultivating microalgae in ocean shoal region described in claim 1, special
Sign is that it includes four vertex spud piles (3) that seabed is fixed in bottom end, and four vertex spud piles (3) are in ocean shoal region
It surrounds rectangle culture zone (2), two relative sides of the rectangle culture zone (2) are respectively equipped with several side spud piles
(15), the side spud pile (15) of every side is equidistantly arranged between the side vertex Liang Gen spud pile (3) and bottom end is solid
Due to seabed；The side spud pile (15) of two relative sides is arranged correspondingly, between each opposite side spud pile (15)
It is fixedly connected with fixed rope (16) in the water that one is located at below the water surface, is also fixedly connected with the fixed rope that is in water (16)
The fixed rope waterborne (10) of surface；Several transverse directions are provided between fixed rope (16) and fixed rope waterborne (10) in water to lead
Tracheae (1) and at least one longitudinal direction gas-guide tube (5) communicated with each item transverse direction gas-guide tube (1)；The system also includes be located at
Several both culturing microalgae polybags (6) in water between fixed rope (16) and fixed rope waterborne (10)；The modeling of each both culturing microalgae
The downside of material bag (6) is all connected with fixed rope in water (16)；Both culturing microalgae polybag (6) is connected to cross by air inlet pipe (7)
To on gas-guide tube (1)；The upper side of both culturing microalgae polybag (6) is also connected with exhaust pipe (9), the inner end of exhaust pipe (9) with it is micro-
Algae cultivation polybag (6) inner cavity communicates, and exhaust pipe (9) connects on fixed rope (10) on the water and true by fixed rope waterborne (10)
It is upward to protect outer end opening；Both culturing microalgae polybag (6) is also fixedly connected with the disengaging of expects pipe (8) for material；The system is also
Including operating console (13), which is fixedly installed in the one of rectangle culture zone (2) by seabed spud pile
Side is equipped with blower (12) on operating console (13)；The longitudinal direction gas-guide tube (5) passes through air valve (11) connection blower (12)；
The vertex spud pile (3) also connects adjacent lateral gas-guide tube (1) both ends of the vertex spud pile (3) by rope (4), leads to
It crosses tension rope (4) and fixed function is played to gas-guide tube.
3. both culturing microalgae system according to claim 2, it is characterised in that: described each lateral gas-guide tube (1) it is isometric and
Be parallel to the long side in rectangle ocean shoal region, longitudinal gas-guide tube (5) through each lateral gas-guide tube (1), longitudinal gas-guide tube (5) with
The central axes of each transverse direction gas-guide tube (1) overlap.
4. both culturing microalgae system according to claim 2, it is characterised in that: the ocean shoal region is equipped with gear wave
The shoal on dam.
5. both culturing microalgae system according to claim 2, it is characterised in that: the inner end of expects pipe (8) is located at both culturing microalgae modeling
Material bag (6) inner cavity, outer end are equipped with sealing-plug (14).
6. according to both culturing microalgae system described in Claims 2 or 3 or 4 or 5, it is characterised in that: sea where rectangle culture zone (2)
The depth of water in foreign shoal region is no more than one meter.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811514606.1A CN109321468A (en) | 2018-12-12 | 2018-12-12 | The method and both culturing microalgae system of cultivating microalgae in a kind of region of ocean shoal |
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Application publication date: 20190212 |