CN109355195A - The method and both culturing microalgae system of cultivating microalgae in a kind of solarization salt pond - Google Patents
The method and both culturing microalgae system of cultivating microalgae in a kind of solarization salt pond Download PDFInfo
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- CN109355195A CN109355195A CN201811469667.0A CN201811469667A CN109355195A CN 109355195 A CN109355195 A CN 109355195A CN 201811469667 A CN201811469667 A CN 201811469667A CN 109355195 A CN109355195 A CN 109355195A
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- 238000012258 culturing Methods 0.000 title claims abstract description 52
- 150000003839 salts Chemical class 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 16
- 241000195493 Cryptophyta Species 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 239000013535 sea water Substances 0.000 claims abstract description 8
- 235000015097 nutrients Nutrition 0.000 claims abstract description 6
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229940033663 thimerosal Drugs 0.000 claims abstract description 6
- 230000003760 hair shine Effects 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 8
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000012267 brine Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 2
- 230000005068 transpiration Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000005791 algae growth Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
- C12M29/22—Oxygen discharge
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Tropical Medicine & Parasitology (AREA)
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Abstract
The invention discloses the methods and its both culturing microalgae system of cultivating microalgae in a kind of solarization salt pond, its main feature is that, this method is that setting is located at liquid level thin hole knitmesh below in solarization salt pond, is dredging the transparent both culturing microalgae polybag that several connection supply air lines are arranged below the knitmesh of hole；Exhaust pipe is arranged in the upper side of both culturing microalgae polybag, and the exhaust pipe is made to be fixed up opening；Both culturing microalgae polybag is additionally provided with disengaging of the expects pipe for algae, algae solution, seawater, nutrient solution and thimerosal；A kind of method and both culturing microalgae system for shining cultivating microalgae in salt pond is provided, both culturing microalgae coverage of water is significantly expanded, the marine environment cultivation of microalgae is realized, mitigates influence of the environment to algae pollution, increase economic efficiency.
Description
Technical field
The present invention relates to a kind of methods of cultivating microalgae, and in particular to a method of shine cultivating microalgae in salt pond.This hair
The bright both culturing microalgae system for further relating to implement the method for cultivating microalgae in the solarization salt pond.
Background technique
Microalgae is a kind of, full of nutrition, photosynthetic availability high autophyte widely distributed in land, ocean, Neng Goutong
Its efficient photosynthesis is crossed, using luminous energy, absorbs CO2And H2O converts them into carbohydrate, protein, fat etc.
Chemical energy, and release O2.Polysaccharide, protein, the pigment etc. that cell metabolism generates, make its food, medicine, genetic engineering,
The fields such as liquid fuel have good development prospect.
Microalgae it is many kinds of and it is individual be generally less than 2mm.Currently, both culturing microalgae mostly uses greatly the open pond in land to be supported
It grows.But the breeding way has the disadvantage in that one, due to needing sufficient illumination in microalga cultivation process, can not pass through increasing
Breeding water body is opened up in the measures such as plus depth, so taking up a large area, farm is limited, it is difficult to realize that large area, scale are supported
It grows.Two, terrestrial environment is poor, easily makes cultivated microalgae contaminated；Three, it is with high salt, high-alkali to be adapted only to fraction for the breeding way
The microalgae of equal extreme environments existence, the type of cultivating microalgae are limited.
Summary of the invention
The technical problem to be solved by the invention is to provide a kind of method of cultivating microalgae in solarization salt pond and both culturing microalgae systems
System significantly expands both culturing microalgae coverage of water, realizes the marine environment cultivation of microalgae, mitigates environment to the shadow of algae pollution
It rings, increases economic efficiency.
In order to solve this technical problem, the invention adopts the following technical scheme:
A kind of method of cultivating microalgae in solarization salt pond, which is characterized in that knitted shining setting in salt pond and being located at liquid level thin hole below
Net is dredging the transparent both culturing microalgae polybag that several connection supply air lines are arranged below the knitmesh of hole；Both culturing microalgae polybag
Upper side exhaust pipe is set, and the exhaust pipe is made to be fixed up opening；Both culturing microalgae polybag is additionally provided with expects pipe for algae
The disengaging of kind, algae solution, seawater, nutrient solution and thimerosal.
For implementing the both culturing microalgae system for shining the method for cultivating microalgae in salt pond, it is characterised in that it includes shining
Salt pond dredges hole knitmesh and console, is equipped with blower on console；It shines and is equipped with several spud piles in salt pond；It knits in the thin hole
Net is connected with spud pile, is tightened up thin hole knitmesh below the liquid level for shining salt pond；Several are equipped with below thin hole knitmesh
Transparent both culturing microalgae polybag；Several lateral gas-guide tubes and equal with each item transverse direction gas-guide tube are additionally provided with below thin hole knitmesh
The longitudinal gas-guide tube of at least one communicated, wherein longitudinal gas-guide tube connects the blower；Both culturing microalgae polybag passes through air inlet pipe
It is connected on lateral gas-guide tube, the upper side of both culturing microalgae polybag is also connected with exhaust pipe；The inner end of exhaust pipe and microalgae are supported
It grows polybag inner cavity to communicate, outer end opening is upward；Both culturing microalgae polybag is also fixedly connected with expects pipe, and the inner end of expects pipe is located at micro-
Algae cultivates polybag inner cavity, and outer end is equipped with sealing-plug.
Preferably, it shines and is also connected with the fixed rope for being located at and dredging above the knitmesh of hole between the spud pile of salt pond front and rear sides；Row
Tracheae is connected on fixed rope and outer end opening is upward.
Preferably, exhaust pipe is connected in thin hole knitmesh and outer end opening is upward.
Preferably, each lateral gas-guide tube is isometric and is parallel to the horizontal edge for shining salt pond, and longitudinal gas-guide tube runs through each cross
To gas-guide tube, the central axes of longitudinal gas-guide tube and each lateral gas-guide tube overlap.
The positive effect of the present invention is:
The present invention is cultivated in bag by the way that microalgae is placed in plastics, and plastics cultivation bag is laid on and is shone in salt pond, is effectively utilized solarization
Both culturing microalgae coverage of water is greatly improved in salt pool area；And can gather materials on the spot, direct extracting seawater cultivating microalgae, more
Add economic convenient, saving labour, while ensure that the stabilization of micro algae growth environment, effectively prevents terrestrial environment to microalgae
Pollution.Mode through the invention can be realized large area artificial breeding microalgae, increase the purpose of culture fishery economic benefit.
It is cultivated by being placed on different microalgae in different polybags, it can be achieved that cultivating a variety of microalgaes simultaneously, the cultivation type of microalgae is not
It is limited again.Because microalgae color is deeper, absorption daylight ability is stronger compared with salt water, by the cultivating microalgae in shining salt pond, can promote solarization
Temperature in salt pond facilitates pond moisture transpiration of evaporating brine, and shortening is evaporated brine the period, improves and produces salt efficiency.
Detailed description of the invention
Fig. 1 is both culturing microalgae system structure diagram of the invention；
Fig. 2 is both culturing microalgae plastic bag structure schematic top plan view of the invention；
Fig. 3 is both culturing microalgae plastic bag structure left view schematic diagram of the invention.
Specific embodiment
The present invention is further illustrated with reference to the accompanying drawings and examples.
Cultural method of the invention are as follows: be located at liquid level thin hole knitmesh below shining setting in salt pond, under thin hole knitmesh
The transparent both culturing microalgae polybag of several connection supply air lines is arranged in side；Exhaust is arranged in the upper side of both culturing microalgae polybag
Pipe, and the exhaust pipe is made to be fixed up opening；Both culturing microalgae polybag is additionally provided with expects pipe for algae, algae solution, seawater, nutrition
The disengaging of liquid and thimerosal.
Both culturing microalgae system embodiment of the invention is as follows:
As shown in Figure 1, the embodiment of present system includes shining salt pond 2 and thin hole knitmesh 4, it further include being located to shine 2 outside of salt pond
Console 13.Blower 12 is installed on console 13.
It shines and is equipped with several spud piles 3 on the inside of 2 edge of salt pond, shine and be also connected with position between the spud pile 3 of 2 front and rear sides of salt pond
Fixed rope 10 above thin hole knitmesh 4.The thin hole knitmesh 4 is connected with spud pile 3, makes to dredge hole knitmesh 4 in solarization salt pond 2
Liquid level below be tightened up.Several transparent both culturing microalgae polybags 6 are equipped with below hole knitmesh 4 dredging.Under thin hole knitmesh 4
At least one longitudinal direction gas-guide tube 5 that side is additionally provided with several lateral gas-guide tubes 1 and communicates with each item transverse direction gas-guide tube 1, wherein
Longitudinal gas-guide tube 5 connects the blower 12 by air valve 11.Each lateral gas-guide tube 1 is isometric and is parallel to solarization salt pond 2
Horizontal edge, longitudinal gas-guide tube 5 overlap through each lateral gas-guide tube 1, the central axes of longitudinal gas-guide tube 5 and each lateral gas-guide tube 1.
Longitudinal gas-guide tube 5, lateral gas-guide tube 1 and both culturing microalgae polybag 6 are all located at the downside of thin hole knitmesh 4, it is therefore an objective to
It is located at this system and shines 2 liquid level of salt pond or less.It prevents from having an adverse effect to the transpiration for shining salt pond 2.
In conjunction with Fig. 1, Fig. 2 and Fig. 3, both culturing microalgae polybag 6 is connected on lateral gas-guide tube 1 by air inlet pipe 7, and microalgae is supported
The upper side for growing polybag 6 is also connected with exhaust pipe 9.The inner end of exhaust pipe 9 is communicated with 6 inner cavity of both culturing microalgae polybag, exhaust
Pipe 9 is connected on fixed rope 10 and ensures that outer end opening is upward by fixed rope 10, prevents from falling because of 9 side of exhaust pipe, and liquid enters
In exhaust pipe 9, guarantee that the gas in both culturing microalgae polybag 6 is smoothly discharged.In practical operation, air inlet pipe 7 and both culturing microalgae are moulded
It is needed between material bag 6 fastened to each other in order to which entirety is put into pond and recycled in its entirety.Both culturing microalgae polybag 6 is also fixedly connected with
Expects pipe 8 is used for the disengaging of algae, algae solution, seawater, nutrient solution and thimerosal, and the inner end of expects pipe 8 is located in both culturing microalgae polybag 6
Chamber, outer end are equipped with sealing-plug 14.
The present invention can not also set fixed rope 10, but exhaust pipe 9 is connected to thin hole knitmesh 4 and by dredging hole knitmesh 4
Ensure that outer end opening is upward.
Algae, seawater, nutrient solution and thimerosal etc. are filled in both culturing microalgae polybag 6 respectively.Open blower 12 and air valve
11, into both culturing microalgae polybag 6 in be passed through air, provide carbon dioxide for the photosynthesis of microalgae.Extra air and light
The oxygen that cooperation generates is discharged upwards by exhaust pipe 9.Sealing-plug 14 is opened, using machinery suction or manual operations, is led to
Charge tube 8 periodically extraction section algae solution and fills into the seawater and nutrient solution of respective volume from both culturing microalgae polybag 6.
Claims (5)
1. a kind of method for shining cultivating microalgae in salt pond, it is characterised in that: be located at liquid level thin hole below shining setting in salt pond
The transparent both culturing microalgae polybag that several connection supply air lines are arranged below the knitmesh of hole is being dredged in knitmesh；Both culturing microalgae plastics
Exhaust pipe is arranged in the upper side of bag, and the exhaust pipe is made to be fixed up opening；Both culturing microalgae polybag is additionally provided with expects pipe and is used for
The disengaging of algae, algae solution, seawater, nutrient solution and thimerosal.
2. for implementing the both culturing microalgae system for shining the method for cultivating microalgae in salt pond described in claim 1, it is characterised in that
It includes shining salt pond (2), dredges hole knitmesh (4) and console (13), is equipped with blower (12) on console (13)；It shines salt pond (2)
In be equipped with several spud piles (3)；The thin hole knitmesh (4) is connected with spud pile (3), makes to dredge hole knitmesh (4) in solarization salt pond
(2) it is tightened up below liquid level；Several transparent both culturing microalgae polybags (6) are equipped with below thin hole knitmesh (4)；In thin hole
At least one longitudinal direction for being additionally provided with several lateral gas-guide tubes (1) below knitmesh (4) and being communicated with each item transverse direction gas-guide tube (1)
Gas-guide tube (5), wherein longitudinal gas-guide tube (5) connects the blower (12)；Both culturing microalgae polybag (6) is connected by air inlet pipe (7)
It connects on lateral gas-guide tube (1), the upper side of both culturing microalgae polybag (6) is also connected with exhaust pipe (9)；Exhaust pipe (9) it is interior
End is communicated with both culturing microalgae polybag (6) inner cavity, and outer end opening is upward；Both culturing microalgae polybag (6) is also fixedly connected with expects pipe
(8), the inner end of expects pipe (8) is located at both culturing microalgae polybag (6) inner cavity, and outer end is equipped with sealing-plug (14).
3. both culturing microalgae system according to claim 2, it is characterised in that: shine the spud pile (3) of salt pond (2) front and rear sides
Between be also connected be located at dredge hole knitmesh (4) above fixed rope (10)；Exhaust pipe (9) is connected on fixed rope (10) and outer
End opening is upward.
4. both culturing microalgae system according to claim 2, it is characterised in that: exhaust pipe (9) is connected on thin hole knitmesh (4)
And outer end opening is upward.
5. according to both culturing microalgae system described in Claims 2 or 3 or 4, it is characterised in that: each lateral gas-guide tube (1)
It is isometric and be parallel to the horizontal edge for shining salt pond (2), longitudinal gas-guide tube (5) through each lateral gas-guide tube (1), longitudinal gas-guide tube (5) with
The central axes of each transverse direction gas-guide tube (1) overlap.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811469667.0A CN109355195A (en) | 2018-11-26 | 2018-11-26 | The method and both culturing microalgae system of cultivating microalgae in a kind of solarization salt pond |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811469667.0A CN109355195A (en) | 2018-11-26 | 2018-11-26 | The method and both culturing microalgae system of cultivating microalgae in a kind of solarization salt pond |
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| CN109355195A true CN109355195A (en) | 2019-02-19 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110499245A (en) * | 2019-08-08 | 2019-11-26 | 浙江万里学院 | A kind of shellfish culture pond is spread cultivation system and its application method in situ with microalgae |
| CN111961569A (en) * | 2020-06-19 | 2020-11-20 | 国投生物科技投资有限公司 | Seawater microalgae culture system and algae culture and sea salt recycling method thereof |
-
2018
- 2018-11-26 CN CN201811469667.0A patent/CN109355195A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110499245A (en) * | 2019-08-08 | 2019-11-26 | 浙江万里学院 | A kind of shellfish culture pond is spread cultivation system and its application method in situ with microalgae |
| CN110499245B (en) * | 2019-08-08 | 2023-06-23 | 浙江万里学院 | Microalgae in-situ expansion culture system for shellfish culture pond and use method thereof |
| CN111961569A (en) * | 2020-06-19 | 2020-11-20 | 国投生物科技投资有限公司 | Seawater microalgae culture system and algae culture and sea salt recycling method thereof |
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Application publication date: 20190219 |