CN205774572U - A kind of culture dish cultivated for mature fat cell ceiling - Google Patents
A kind of culture dish cultivated for mature fat cell ceiling Download PDFInfo
- Publication number
- CN205774572U CN205774572U CN201620453826.8U CN201620453826U CN205774572U CN 205774572 U CN205774572 U CN 205774572U CN 201620453826 U CN201620453826 U CN 201620453826U CN 205774572 U CN205774572 U CN 205774572U
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- culture dish
- tube
- fat cell
- mature fat
- cultivated
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Abstract
The utility model discloses a kind of culture dish cultivated for mature fat cell ceiling, belong to biological medicine experimental provision technical field, in the big culture dish that forward is placed, one or several inverted little culture dish are set, and U-tube is set in big culture dish, one end is exposed to outside the culture fluid of big culture dish, one end is positioned at inverted little culture dish, it is ensured that the inner bottom part of inverted little culture dish is without air remaining, and in simultaneously facilitating inverted little culture dish, liquid exchanges with ambient atmos.Meanwhile, U-tube can be connected with sample adding device, cultivates in ripe adipose cell is expelled to inverted little culture dish.This culture dish reasonable in design, simple to operate, it is possible to effectively to cultivate mature fat cell.
Description
Technical field
This utility model belongs to biological medicine experimental provision technical field, relates to a kind of culture dish, specifically relates to
And a kind of for mature fat cell ceiling cultivate culture dish.
Background technology
Adipose cell has important function in organism metabolism regulates and controls, and it serves not only as the storage of energy matter
Unit, and actively regulate the organism metabolism under Different Nutrition state by release active factors.Fat is thin
Born of the same parents' dysfunction is fat etc. as caused by lipid excess accumulation has a strong impact on human health.In vitro culture fat is thin
Born of the same parents and then to further investigate its function be the important content in current endocrine metabolism field.Because becoming
Ripe adipose cell contains triglyceride, and density is little compared with aqueous solution, is suspended in culture fluid surface in cultivation, because of
This, mature fat cell is cultivated and is used ceiling method more.But, existing carry out ceiling cultivation
Utensil is mainly the culture bottle of commercialization, there is not yet the culture dish cultivated for ceiling.Test at some
In, culture bottle is difficult to meet the operation requirement of experiment, and culture dish has irreplaceable advantage.
Therefore, a kind of culture dish that can be used in cultivating mature fat cell is needed badly.
Utility model content
The purpose of this utility model is to provide a kind of culture dish cultivated for mature fat cell ceiling,
This culture dish reasonable in design, simple to operate, it is possible to effectively to cultivate mature fat cell.
This utility model be employed technical scheme comprise that, a kind of for mature fat cell ceiling cultivate
Culture dish, including big culture dish and one or several little culture dish being inverted in big culture dish, also includes
One or several U-tube, U-tube one end is stretched in inverted little culture dish, and the other end stretches out big cultivation
The surface of culture fluid in ware.
Feature of the present utility model also resides in,
The lower edge of little culture dish is placed in U-tube.
The caliber of U-tube is 1～3mm.
The sidewall of little culture dish is additionally provided with clamping part.
The opening of wide 2～4mm is offered at the ware mouth of little culture dish.
Described U-tube quantity is 3,3 U-tube equidistant arrangement.
U-tube is stretched out the one end on culture fluid surface and is connected with sample adding device.
Big culture dish top is additionally provided with culture dish top cover.
Compared with prior art, this utility model has a following useful technique effect:
The disclosed culture dish cultivated for mature fat cell ceiling of this utility model, places at forward
Big culture dish in one or several inverted little culture dish are set, also include one or several U-tube,
U-tube one end is exposed to outside the culture fluid of big culture dish, and one end is positioned at inverted little culture dish, it is ensured that
The inner bottom part of inverted little culture dish, without air remaining, can promote liquid in inverted little culture dish simultaneously
Exchange with ambient atmos.This culture dish reasonable in design, simple to operate, it is possible to effectively to cultivate ripe fat
Fat cell.
Further, the lower edge of little culture dish is placed in U-tube, owing to the caliber of U-tube is 1～3mm,
Therefore, from periphery there is 1 millimeter or several millimeters in big culture dish inner bottom to make the ware mouth of inverted little culture dish
Distance, make the adipose cell can gas exchange abundant with the external world, it is ensured that oxygen supply and CO2Discharge.
Further, the sidewall of inverted little cultivation is provided with clamping part, or offers at its ware mouth
Wide 2～the opening of 4mm, it is easy to clamping, convenient take out inverted little culture dish.Meanwhile, peripheral big training
The bottom supporting ware and the little culture dish of internal inversion is the thinnest, it is easy to the observation of cell.
Further, U-tube is stretched out the one end on culture fluid surface and is connected with sample adding device, convenient to inversion
Little culture dish carry out the conveying of adipose cell and culture fluid.
Accompanying drawing explanation
Fig. 1 is the structural representation of this utility model embodiment 1.
Wherein: 1. big culture dish, the least culture dish, 3.U type pipe, 4. clamping part, 5. culture dish top cover.
Detailed description of the invention
With detailed description of the invention, this utility model is described in detail below in conjunction with the accompanying drawings.
Embodiment 1
Seeing Fig. 1, a kind of culture dish cultivated for mature fat cell ceiling, including big culture dish
1 and be inverted in big culture dish 1 or several little culture dish 2, also include one or several U-shaped
Pipe 3, U-tube 3 one end is stretched in inverted little culture dish 2, and the other end stretches out in big culture dish to be cultivated
The surface of liquid.The lower edge of little culture dish 2 is placed in U-tube 3, and the caliber of U-tube 3 is 1～3mm,
U-tube 3 is stretched out the one end on culture fluid surface and is connected with sample adding device.Big culture dish 1 top is additionally provided with training
Support ware top cover 5.
The sidewall of little culture dish 2 is additionally provided with clamping part 4.Or, at the ware mouth of little culture dish 2
Offer the opening of wide 2～4mm.
Preferably, U-tube 3 quantity is 3,3 U-tube 3 equidistant arrangement.
The culture dish that this utility model is cultivated for mature fat cell ceiling, in use:
Inverted one or several little culture dish 2 are positioned in big culture dish 1, it is ensured that U-tube 3 low side
It is placed in inverted little culture dish 2.Add culture fluid in big culture dish 1, until inverted little cultivation
Ware 2 is internal is completely filled with culture fluid.Sample adding device is used to be injected by 3 mature fat cells of U-tube
Cultivate in inverted little culture dish 2.
Claims (8)
1. the culture dish cultivated for mature fat cell ceiling, it is characterised in that include training greatly
Support ware (1) and be inverted in big culture dish (1) or several little culture dish (2), also including one
Individual or several U-tube (3), U-tube (3) one end is stretched in inverted little culture dish (2), another
End stretches out the surface of culture fluid in big culture dish.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, the lower edge of little culture dish (2) is placed in U-tube (3).
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, the caliber of U-tube (3) is 1～3mm.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levy and be, the sidewall of little culture dish (2) is additionally provided with clamping part (4).
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levy and be, at the ware mouth of little culture dish (2), offer the opening of wide 2～4mm.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, described U-tube (3) quantity is 3,3 U-tube (3) equidistant arrangement.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, U-tube (3) is stretched out the one end on culture fluid surface and is connected with sample adding device.
The culture dish cultivated for mature fat cell ceiling the most according to claim 1, it is special
Levying and be, big culture dish (1) top is additionally provided with culture dish top cover (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620453826.8U CN205774572U (en) | 2016-05-18 | 2016-05-18 | A kind of culture dish cultivated for mature fat cell ceiling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620453826.8U CN205774572U (en) | 2016-05-18 | 2016-05-18 | A kind of culture dish cultivated for mature fat cell ceiling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205774572U true CN205774572U (en) | 2016-12-07 |
Family
ID=58120194
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620453826.8U Expired - Fee Related CN205774572U (en) | 2016-05-18 | 2016-05-18 | A kind of culture dish cultivated for mature fat cell ceiling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205774572U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838604A (en) * | 2016-05-18 | 2016-08-10 | 西安医学院 | Culture dish for mature adipocyte ceiling culture |
-
2016
- 2016-05-18 CN CN201620453826.8U patent/CN205774572U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838604A (en) * | 2016-05-18 | 2016-08-10 | 西安医学院 | Culture dish for mature adipocyte ceiling culture |
| CN105838604B (en) * | 2016-05-18 | 2018-07-06 | 西安医学院 | For the culture dish of mature fat cell ceiling culture |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161207 Termination date: 20190518 |