CN202347020U - Chicken embryo petri dish - Google Patents
Chicken embryo petri dish Download PDFInfo
- Publication number
- CN202347020U CN202347020U CN2011202660031U CN201120266003U CN202347020U CN 202347020 U CN202347020 U CN 202347020U CN 2011202660031 U CN2011202660031 U CN 2011202660031U CN 201120266003 U CN201120266003 U CN 201120266003U CN 202347020 U CN202347020 U CN 202347020U
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- China
- Prior art keywords
- yolk
- chicken embryo
- petri dish
- chicken
- carrier
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- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model relates to a chicken embryo petri dish which comprises a dish body and a dish cover, wherein the dish body is provided with a yolk receiving part. The chicken embryo petri dish disclosed by the utility model has the advantages that through arrangement of the yolk receiving part, a yolk sac is positioned at the centre of the petri dish, and the upper part of the yolk sac is prevented from getting close to the wall of the petri dish and the growth of upper vessels cannot be affected; since the yolk receiving part is small in size, most of the egg white exists at the upper part of the petri dish, the yolk sac can be totally covered by the egg white with the rise of liquid level, so that vessels growing at the upper part of the yolk sac are not affected or slightly affected by evaporation and other factors during the culture process, thereby imitating the real growing environment of an embryo egg to the maximum extent and facilitating the culture of the chicken embryo. In addition, the chicken embryo petri dish is provided with a thermal insulation device, the death of the chicken embryos, caused by temperature difference, can be reduced when the chicken embryos are taken out to be observed, and the survival rate of the chicken embryos is increased; the chicken embryo petri dish is simple in structure convenient to use, low in cost, and suitable for popularization and application.
Description
Technical field
The utility model relates to a kind of medical experiment vessel, specifically, is a kind of chicken embryo culture ware that is used for the chicken embryo culture.
Background technology
Present existing cultivation in the chick embryo mainly contains two kinds:
1, direct egg culture method: adopt fertilization instar chicken embryo on the 5th; The blastular position is confirmed in darkroom spot light lamp irradiation; Crack diameter 3cm left and right sides eggshell in the blastular position as observing and place the medicine window; Put into 37-38 ℃ of incubator and cultivate, 8-10 day splashes into window with fixing agent, kills the chick chorioallantoic membrane (CAM) that takes out about the about 5cm of diameter behind the chicken embryo and takes pictures or the immunohistochemical methods detection;
2, traditional 100mm diameter petridish culture method: popular in recent years chicken embryo culture method.Adopt 100mm diameter Tissue Culture Dish simulation chicken embryonic development environment, directly 2-3 age in days embryo egg is cracked, pour petridish into, put into 37-38 ℃ of incubator and cultivate, but direct viewing is taken pictures and the dosing intervention.
But there are many deficiencies in existing cultural method:
1, direct egg culture method: this method can not be observed chicken embryo vessel growth situation at any time; When dosing is intervened; The placement of pharmaceutical carrier can not precisely be located, can not direct viewing for the influence of angiogenic growth, seldom use at present as chicken embryo culture model;
2, traditional 100mm diameter petridish culture method: this method can direct viewing chicken embryo vessel growth situation, is the most frequently used cultural method at present.But because traditional 100mm diameter petridish area is bigger; Egg white can not wrap up yolk fully; Yolk is higher than the fluid level; Therefore and the chick chorioallantoic membrane angiogenic growth is usually located at the yolk top, causes the dry shrinkage of top blood vessel when cultivating owing to reasons such as evaporations, the not good enough chicken embryo decrease in survival rate that causes of angiogenic growth environment; Yolk position uncertain; Existing chicken embryo culture method is to knock eggshell open content integral body is poured into petridish; The tradition petridish can be because the uncertain yolk that causes in the position in the toppling process be pressed close to petridish wall possibility; And adherent meeting causes the angiogenic growth spatial to dwindle, and further causes the necrosis of adherent blood vessel, thereby chicken embryo embryo blood supply insufficiency is dead; Must more frequently from incubator, take out petridish when taking pictures owing to experimental observation in addition; The tradition petridish is to aim at the cell cultures design, do not have heat-insulation system, and the chicken embryonic development is very responsive to variation of temperature; Frequent pick and place the change that must cause temperature, cause declining to a great extent of chicken embryo survival rate.Because there is multiple instability in the condition of survival of chicken embryo and angiogenic growth that influences in traditional cultural method, also increased the instability of observation index, has disturbed the objectivity of experiment.
Therefore, need badly and want a kind of simple in structure, effectively the petridish of culturing chicken embryo still about this chicken embryo culture ware, does not also appear in the newspapers at present.
Summary of the invention
The purpose of the utility model is that a kind of chicken embryo culture ware is provided.
For realizing above-mentioned purpose, the technical scheme that the utility model is taked is: a kind of chicken embryo culture ware, be provided with ware body and ware lid, and described ware body is provided with the yolk carrier.
Described chicken embryo culture ware is provided with attemperator.
Described yolk carrier is located at petridish central authorities, and carrier is that arc is connected with the petridish junction.
Described yolk carrier becomes cylindrical.
Described attemperator becomes cylindrical, and the attemperator middle part is provided with cylindrical depression.
Described chicken embryo culture ware opening diameter is 7-8 centimetre, the high 2-4 of petridish centimetre.
3-5 centimetre of described yolk carrier diameter, the high 0.5-1.5 of yolk carrier centimetre.
9-10 centimetre of described attemperator diameter, high 4-5 centimetre, 7-8 centimetre of attemperator interior cylindrical recess diameter, high 2.5-3.5 centimetre.
The utility model advantage is:
1, the utility model is provided with the yolk carrier, can yolk sac be positioned petridish central authorities, prevents that yolk sac top from pressing close to the petridish wall, influences the top vessel growth;
2, the utility model is because yolk carrier volume is less; Can make most of egg white be present in the top petridish; The rising of liquid level can make yolk sac integral body covered by egg white; The blood vessel that has guaranteed to grow in the culturing process yolk sac top does not receive or is evaporated less etc. the influence of factor, has simulated the real growing environment of embryo egg to the full extent, more helps the cultivation of chicken embryo;
3, the junction is that arc is connected between the utility model yolk carrier and the ware body, and this connection can be avoided producing wedge angle between yolk carrier and the ware body, prevents to be punctured when yolk from squeezing into petridish;
4, the utility model is provided with attemperator, when taking-up chicken embryo is observed, can reduce because the chicken embryo death that the temperature difference causes increases chicken embryo survival rate;
5, the utility model is simple in structure, and is easy to use, and preparation cost is low, is fit to promote the use of.
Description of drawings
Accompanying drawing 1 is a kind of chicken embryo culture of the utility model ware structural representation.
Accompanying drawing 2 is along Figure 1A-A line diagrammatic cross-section.
Accompanying drawing 3 is another structural representations of a kind of chicken embryo culture of the utility model ware ware body.
Embodiment
Below in conjunction with embodiment and with reference to accompanying drawing the utility model is further described.
The Reference numeral and the integral part that relate in the accompanying drawing are as follows:
1. ware body 11. yolk carriers
111. arc connects 2. wares lid
3. attemperator 31. caves in
Please with reference to Fig. 1, Fig. 1 is a kind of chicken embryo culture of the utility model ware structural representation, and described chicken embryo culture ware is provided with ware body 1, ware lid 2 and attemperator 3; Described ware body 1 is provided with yolk carrier 11; Yolk carrier 11 is located at ware body 1 central authorities, and 11 one-tenth of yolk carriers are cylindrical, and rounded face that yolk carrier 11 is connected with ware body 1 and ware body 1 are concentric(al) circles; 3 one-tenth of said attemperators are cylindrical, and attemperator 3 middle parts are provided with cylindrical depression 31.Please with reference to Fig. 2, Fig. 2 is along Figure 1A-A line diagrammatic cross-section, and said ware body 1 is connected 111 with yolk carrier 11 through arc and connects.Please with reference to Fig. 3, Fig. 3 is another structural representation of a kind of chicken embryo culture of the utility model ware ware body, described yolk carrier 11 bottom ovalization.
Need to prove that described ware body 1 opening diameter is 7-8 centimetre, 2-4 centimetre of ware height, the petridish of this size mainly act as provides the yolk sac growing environment, the volume that broader ware open area can not disturb yolk sac to grow gradually and enlarge; Described yolk carrier 11 rounded face diameters are 3-5 centimetre, high 0.5-1.5 centimetre, and yolk carrier 11 volumes of this size and 2-3 age in days yolk sizableness; Can yolk sac be positioned ware body 1 central authorities; Prevent that yolk sac top from pressing close to the petridish wall, influence the top vessel growth, and avoided striking when getting egg; Because it is uncertain to drop in the yolk position; Many times can lecithic adherent phenomenon, along with the yolk growth will cause the blood vessel of adherent part to lack the growing space, further will cause chicken embryo death; The design of yolk carrier 11; Because the lower section volume is less; Can make most of egg white be present in the top petridish, the rising of liquid level can make yolk sac integral body covered by egg white, and the blood vessel that has guaranteed to grow in the culturing process yolk sac top does not receive or evaporated less etc. the influence of factor; Simulate the real growing environment of embryo egg to the full extent, prolonged the incubation time of chicken embryo greatly; Be connected 11 through arc between yolk carrier 11 and the ware body 1 and connect, this arc connects 111 can be avoided producing wedge angle between yolk carrier 11 and the ware body 1, prevents that yolk from being punctured; Described yolk carrier 11 bottom ovalization can better be imitated egg-shapes, guarantee the normal cultured of chicken embryo; Described attemperator 3 diameter 9-10 centimetres, high 4-5 centimetre, attemperator 3 interior cylindrical cave in 31 diameter 7-8 centimetres; High 2.5-3.5 centimetre, said depression 31 can be held ware body 1 and yolk carrier 11, and said attemperator 3 is processed for glass; In the attemperator 3 water is housed; Because it is higher that specific heat of water holds, after reaching certain temperature, can not lower the temperature very soon, thereby play insulation effect because of environmental change.
The utility model at first is preheated to attemperator 3 about 38 degree in use, in the petridish of washing and sterilizing, squeezes into egg then; Yolk flows in the yolk carrier 11, and egg white can cover yolk, covers ware lid 2; Petridish is placed attemperator 3 interior cultivations of preheating; During observation, attemperator 3 and petridish are together taken out, can prevent the death of chicken embryo because of the influence of circumstance of temperature difference.The utility model is simple in structure, and is easy to use, can effectively increase the cultivation and the observing time of chicken embryo, created good animal model for cell cultures etc., and therefore, the utility model can be promoted the use of, for certain contribution is made in scientific experiment and research.
The above only is the preferred implementation of the utility model; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the utility model principle; Some improvement be can also make and these improvement and the additional protection domain that also should be regarded as the utility model replenished.
Claims (8)
1. a chicken embryo culture ware is provided with ware body and ware lid, it is characterized in that described ware body is provided with the yolk carrier.
2. chicken embryo culture ware according to claim 1 is characterized in that, described chicken embryo culture ware is provided with attemperator.
3. chicken embryo culture ware according to claim 1 is characterized in that described yolk carrier is located at petridish central authorities, and carrier is that arc is connected with the petridish junction.
4. chicken embryo culture ware according to claim 1 is characterized in that described yolk carrier becomes cylindrical.
5. chicken embryo culture ware according to claim 2 is characterized in that described attemperator becomes cylindrical, and the attemperator middle part is provided with cylindrical depression.
6. chicken embryo culture ware according to claim 1 is characterized in that, described chicken embryo culture ware opening diameter is 7-8 centimetre, the high 2-4 of petridish centimetre.
7. chicken embryo culture ware according to claim 1 is characterized in that, 3-5 centimetre of described yolk carrier diameter, the high 0.5-1.5 of yolk carrier centimetre.
8. chicken embryo culture ware according to claim 2 is characterized in that, 9-10 centimetre of described attemperator diameter, high 4-5 centimetre, 7-8 centimetre of attemperator interior cylindrical recess diameter, high 2.5-3.5 centimetre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202660031U CN202347020U (en) | 2011-07-26 | 2011-07-26 | Chicken embryo petri dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202660031U CN202347020U (en) | 2011-07-26 | 2011-07-26 | Chicken embryo petri dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202347020U true CN202347020U (en) | 2012-07-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011202660031U Expired - Fee Related CN202347020U (en) | 2011-07-26 | 2011-07-26 | Chicken embryo petri dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202347020U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104099246A (en) * | 2014-06-24 | 2014-10-15 | 孙海翔 | Minitype incubator |
| CN106635793A (en) * | 2017-02-07 | 2017-05-10 | 重庆科技学院 | Culture dish for ultrasonic experiment and use method |
-
2011
- 2011-07-26 CN CN2011202660031U patent/CN202347020U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104099246A (en) * | 2014-06-24 | 2014-10-15 | 孙海翔 | Minitype incubator |
| CN104099246B (en) * | 2014-06-24 | 2016-03-16 | 孙海翔 | Miniature couveuse |
| CN106635793A (en) * | 2017-02-07 | 2017-05-10 | 重庆科技学院 | Culture dish for ultrasonic experiment and use method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120725 Termination date: 20130726 |