CN106706861A - Reaction effect detection device capable of simulating environment of intestinal and gastric fluids of human body - Google Patents
Reaction effect detection device capable of simulating environment of intestinal and gastric fluids of human body Download PDFInfo
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- CN106706861A CN106706861A CN201710071894.7A CN201710071894A CN106706861A CN 106706861 A CN106706861 A CN 106706861A CN 201710071894 A CN201710071894 A CN 201710071894A CN 106706861 A CN106706861 A CN 106706861A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K13/00—Thermometers specially adapted for specific purposes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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Abstract
The invention relates to a reaction effect detection device capable of simulating an environment of intestinal and gastric fluids of a human body, and is used to simulate the reaction of intestinal and gastric fluids. The reaction effect detection device comprises a reaction vessel and an air-tight mechanism, the reaction vessel is connected with the air-tight mechanism in an inserted manner through an interface, a sample inlet and an acid-base regulation opening are formed in the upper part of the reaction vessel, an air inlet and an air outlet are formed in the middle part of the air-tight mechanism, an upper opening is formed in the top part of the air-tight mechanism, a rubber plug is arranged in the upper opening, a pH value electrode and a temperature probe are fixed in the rubber plug, and the pH value electrode and one end of the temperature probe respectively extend into the bottom part of the reaction vessel. The reaction environment is completely anaerobic, the temperature and the pH value are monitored in real time, the installation is convenient, the dismantling is simple, and the device is suitable for industrial production.
Description
Technical field
The present invention provides a kind of reactor, can particularly simulate the reaction effect detection means in people's stomach pendular ring border, and category is surveyed
Examination technical field.
Background technology
When research something is to the biological effectiveness of human body, in vitro test, mould generally are carried out using simulating stomach liquid and react
Intend the reaction of stomach liquid and be commonly used to the materials such as detection medicine or heavy metal to the issuable effect of human body or the extent of injury.
The toxicity assessment of pollutant is always the important content in Research of Environmental Sciences field, the method by simulating stomach liquid
Can exposed level provides positive evidence in human body for pollutant.
Simulation stomach liquid reaction needs simulation human body stomach pendular ring border to be reacted, that is, need to simulate as gastric juice, intestinal juice,
Reaction temperature and pH value, and real-time monitoring is carried out to it, when adding reagent, extracting reactant, also want lucifuge and isolation air.
Existing reaction unit is only capable of completely cutting off air by limited sealing, but in secondary dosing, reaction midway regulation pH value, measurement
The entrance of air is inevitably caused during temperature and pH value, it is impossible to provide a simulated environment for complete anaerobism.
In addition, existing reaction unit is only capable of by artificial timing (generally 15min is once) measurement temperature and pH value, it is impossible to real
When monitoring temperature and pH value, it is not only laborious time-consuming, also increase experimental error.
The content of the invention
A kind of drawback of the present invention for prior art, there is provided reaction effect detection dress for simulating people's stomach pendular ring border
Put, it is capable of the environment of true simulation stomach, realizes the state of isolation of complete anaerobism, and temperature and pH value can be carried out in real time
Monitoring.
Technical problem of the present invention is solved by the following technical programs：
A kind of reaction effect detection means for simulating people's stomach pendular ring border, including reaction utensil, the reaction utensil top sets
There are injection port and acid-base accommodation mouthful, set up tight mechanism, the tight mechanism is spherical cavity, and spherical cavity middle part is provided with air inlet
Mouthful and gas outlet, tight mechanism top is provided with suitable for reading, and bottom is provided with the tight mechanism docking port for leading to reaction utensil, upper intraoral to be provided with
Rubber stopper, tight mechanism docking port passes through interface grafting with reaction utensil.
The above-mentioned reaction effect detection means for simulating people's stomach pendular ring border, is provided with pH value electrode with temperature in the rubber stopper
The socket of probe is spent, the pH value electrode stretches to reaction utensil bottom respectively after passing through socket with temp probe.
The above-mentioned reaction effect detection means for simulating people's stomach pendular ring border, reaction utensil bottom is provided with magnetic stirring apparatus, institute
State and stirrer is provided with reaction utensil.
The outer wall of the above-mentioned reaction effect detection means for simulating people's stomach pendular ring border, the injection port and acid-base accommodation mouth
Inside upper part is provided with stopper, and inside outer wall bottom is provided with bottle cover, and the bottle cover includes bottle cover wall and n bar apron strips, the baffle plate
Bar is α with the angle of horizontal plane, and wherein n is 5-9, and α is 50-60 °.
The above-mentioned reaction effect detection means for simulating people's stomach pendular ring border, light shield is provided with outside the reaction utensil.
The above-mentioned reaction effect detection means for simulating people's stomach pendular ring border, the air inlet is provided with flowmeter.
The above-mentioned reaction effect detection means for simulating people's stomach pendular ring border, the interface is frosting.
The present invention has advantages below：
The present invention by setting tight mechanism, inert gas can be passed through to continual in reaction utensil, so react into
During row, inert gas is filled with from start to finish in the cavity of tight mechanism and in the cavity of reaction utensil, reaction is existed all the time
Carried out in the environment of complete starvation, so as to simulate the anaerobic state of stomach internal organs, prevent reactant from being carried out instead with oxygen
Should, improve the accuracy of experiment.The present invention is convenient to add at any time in experimentation by setting injection port and acid-base accommodation mouthful
Plus material, in addition, sample introduction is separately carried out with acid-base accommodation, it also avoid cross-infection.The present invention installs temperature in reaction utensil
Probe and pH value electrode, can carry out real-time monitoring to temperature and pH value.In addition, various pieces design composition of the invention
Body formula, each several part is detachable, facilitates cleaning.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is reaction utensil structural representation of the present invention；
Fig. 3 is tight mechanism structural representation of the present invention；
Fig. 4 is injection port of the present invention and acid-base accommodation mouth structure schematic diagram；
Fig. 5 is bottle cover deployed configuration schematic diagram of the present invention.
Each label is expressed as in figure：1st, light shield, 2, reaction utensil, 3, magnetic stirring apparatus, 4, injection port, 5, soda acid
Regulation mouth, 6, interface, 6-1, reaction utensil interface, 6-2, tight mechanism docking port, 7, air inlet, 8, gas outlet, 9, tight mechanism,
10th, it is suitable for reading, 11, pH value electrode, 12, temp probe, 13, rubber stopper, 14, stirrer, 15, flowmeter, 16, temperature adjustment button,
17th, rotational speed regulation button, 18, magnetic stirring apparatus switch, 19, stopper, 20, bottle cover, 21, apron strip, 22, outer wall, 23, bottle cover wall.
Specific embodiment
Refering to accompanying drawing 1, the present invention includes reaction utensil 2 and tight mechanism 9, and the top of the reaction utensil 2 is provided with injection port 4 with acid
Alkali adjusts mouth 5, and the tight mechanism 9 is spherical cavity, and spherical cavity middle part is provided with air inlet 7 and gas outlet 8, tight mechanism 9
Top is that the socket for being provided with pH value electrode 11 and temp probe 12 is provided with rubber stopper 13, rubber stopper 13 in suitable for reading 10, suitable for reading 10,
The pH value electrode 11 and temp probe 12 are visited through the bottom for stretching to reaction utensil 2 after socket respectively, pH value electrode 11 with temperature
First 12 pH value and temperature being used in real-time monitoring reaction utensil 2, it is ensured that the environment of reaction utensil is all the time in simulation human body stomach liquid
In environment, reaction utensil 2 passes through the grafting of interface 6 with tight mechanism 9.
In reaction, by being filled with nitrogen or inert gas in air inlet 7, after gas is full of reactor, by the row of gas outlet 8
Go out, to realize the purpose of reaction isolation air.
Refering to accompanying drawing 1, the bottom of reaction utensil 2 is provided with magnetic stirring apparatus 3, and the inner bottom part of the reaction utensil 2 is provided with stirrer 14, uses
Solution in stirring reaction ware 2, is provided with light shield 1 outside the reaction utensil 2, it is ensured that reaction is carried out in the environment of lucifuge,
And having certain insulation effect, air inlet 7 is provided with flowmeter 15, to control the flow of gas.
Refering to accompanying drawing 2, the interface 6 is frosting.Magnetic stirring apparatus 3 is provided with temperature adjustment button 16, rotational speed regulation button
17, magnetic stirring apparatus switch 18, convenient control reaction environment condition.
Refering to accompanying drawing 4,5, the injection port 4 is provided with stopper with the inside upper part of outer wall 22 of acid-base accommodation mouthful 5, in outer wall 22
Side lower part is provided with bottle cover 20, and the bottle cover 20 includes bottle cover wall 23 and n bars apron strip 21, and apron strip 21 is arranged on bottle cover wall 23
On, injection port 4 and acid-base accommodation mouthfuls 5 is taper, and bottle cover is also taper, bottle cover respectively with injection port 4 and the chi of acid-base accommodation mouthful 5
Very little matching, the apron strip 21 is α with the angle of horizontal plane, and wherein n is 5-9, and α is 50-60 °, and apron strip 21 can be linear pattern,
Can also be the arc line shaped of bending, when apron strip 21 is arc line shaped, the radian of bending is 1-45 °, when opening stopper 19 is to anti-
When answering addition reactant in ware 2, nitrogen can be flowed out by injection port 4 or acid-base accommodation mouthful 5, and apron strip is with the horizontal α angles, nitrogen
Helical airflow is formed in the presence of inclined apron strip, prevents the oxygen when reactant is added from entering reaction utensil 2, influence is anti-
Should.
In use, after connecting device, vaseline is smeared to the junction of each vessel, with better seal,
Light shield 1 is enclosed within the outside of reaction utensil 2, by injection port 4 to the gastric juice for adding sample to be configured with 30ml in reaction utensil 2,
PH value electrode 11 and temp probe 12 are fixed on rubber stopper 13, rubber stopper 13 is fixed on suitable for reading 10, then reaction utensil 2 with
The grafting of tight mechanism 9, reaction utensil interface 6-1 inner surfaces are frosting, and tight mechanism docking port 6-2 outer surfaces are frosting, are inserted
After connecting, while reaction utensil 2 is with 9 airtight connection of tight mechanism, convenient dismounting.Then by said apparatus as magnetic stirring apparatus
On 3, temperature adjustment button 16 is opened, adjust the temperature to 37 DEG C, then open rotational speed regulation button 17, adjust suitable rotating speed, finally led to
Enter nitrogen or inert gas.
Appropriate certain density hydrochloric acid (HCl), regulation pH value to 1.5 are added by acid-base accommodation mouthful 5.Observation pH value, if
There is floating, add appropriate amount of acid (or alkali).After 1h, take 10ml solution from injection port 4 with disposable dropper and preserve for follow-up study,
The intestinal juice that 30ml is prepared is added, appropriate NaOH (NaOH) solution is added by acid-base accommodation mouthful 5, regulation pH value is
7.0.Observation pH value, if there is floating, adds appropriate amount of acid (or alkali).After 4h, solution is taken out.All the time nitrogen is passed through in whole experiment process
Gas or inert gas, to completely cut off air, and temperature stabilization at 37 DEG C.
Claims (7)
1. a kind of reaction effect detection means for simulating people's stomach pendular ring border, including reaction utensil (2), on the reaction utensil (2)
Portion is provided with injection port (4) with acid-base accommodation mouthful (5), it is characterised in that set up tight mechanism (9), the tight mechanism (9) is ball
Shape cavity, spherical cavity middle part is provided with air inlet (7) and gas outlet (8), and tight mechanism (9) top is provided with (10) suitable for reading, bottom
It is provided with and rubber stopper (13) is provided with the tight mechanism docking port (6-1) for leading to reaction utensil (2), (10) suitable for reading, tight mechanism docking
Mouth (6-1) is with reaction utensil (2) by interface (6) grafting.
2. a kind of reaction effect detection means for simulating people's stomach pendular ring border according to claim 1, it is characterised in that
The socket of pH value electrode (11) and temp probe (12) is provided with the rubber stopper (13), the pH value electrode (11) is visited with temperature
Head (12) is through the bottom for stretching to reaction utensil (2) after socket respectively.
3. a kind of reaction effect detection means for simulating people's stomach pendular ring border according to claim 2, it is characterised in that
Reaction utensil (2) bottom is provided with magnetic stirring apparatus (3), the reaction utensil (2) and is provided with stirrer (14).
4. a kind of reaction effect detection means for simulating people's stomach pendular ring border according to claim 3, it is characterised in that
The injection port (4) is provided with stopper with outer wall (22) inside upper part of acid-base accommodation mouthful (5), and outer wall (22) lower inside is provided with bottle
Set (20), the bottle cover (20) includes the angle of bottle cover wall (23) and n bars apron strip (21), the apron strip (21) and horizontal plane
It is α, wherein n is 5-9, α is 50-60 °.
5. a kind of reaction effect detection means for simulating people's stomach pendular ring border according to claim 4, it is characterised in that
Light shield (1) is provided with outside the reaction utensil (2).
6. a kind of reaction effect detection means for simulating people's stomach pendular ring border according to claim 5, it is characterised in that
The air inlet (7) is provided with flowmeter (15).
7. a kind of reaction effect detection means for simulating people's stomach pendular ring border according to claim 6, it is characterised in that
The interface (6) is frosting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710071894.7A CN106706861B (en) | 2017-02-09 | 2017-02-09 | A kind of reaction effect detection device in analog people stomach pendular ring border |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710071894.7A CN106706861B (en) | 2017-02-09 | 2017-02-09 | A kind of reaction effect detection device in analog people stomach pendular ring border |
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| CN106706861A true CN106706861A (en) | 2017-05-24 |
| CN106706861B CN106706861B (en) | 2019-07-23 |
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| CN201710071894.7A Active CN106706861B (en) | 2017-02-09 | 2017-02-09 | A kind of reaction effect detection device in analog people stomach pendular ring border |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109975524A (en) * | 2019-05-08 | 2019-07-05 | 江苏省环境科学研究院 | It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method |
| CN110675704A (en) * | 2019-11-20 | 2020-01-10 | 天津开发区合普工贸有限公司 | Convenient intestinal static substitution experimental device |
| CN110890008A (en) * | 2019-12-09 | 2020-03-17 | 天津开发区合普工贸有限公司 | Static type stomach function external substitution experimental device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109975524A (en) * | 2019-05-08 | 2019-07-05 | 江苏省环境科学研究院 | It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method |
| CN110675704A (en) * | 2019-11-20 | 2020-01-10 | 天津开发区合普工贸有限公司 | Convenient intestinal static substitution experimental device |
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| CN110890008A (en) * | 2019-12-09 | 2020-03-17 | 天津开发区合普工贸有限公司 | Static type stomach function external substitution experimental device |
| CN110890008B (en) * | 2019-12-09 | 2024-05-28 | 天津开发区合普工贸有限公司 | Static gastric function in-vitro substitution experimental device |
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