CN109975524A - It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method - Google Patents
It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method Download PDFInfo
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- 238000004088 simulation Methods 0.000 title claims abstract description 50
- 210000002784 stomach Anatomy 0.000 title claims abstract description 44
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000009781 safety test method Methods 0.000 title claims abstract description 10
- 230000001175 peptic effect Effects 0.000 claims abstract description 47
- 239000007788 liquid Substances 0.000 claims abstract description 33
- 230000029087 digestion Effects 0.000 claims abstract description 30
- 238000012544 monitoring process Methods 0.000 claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 12
- 230000002496 gastric effect Effects 0.000 claims abstract description 9
- 230000028327 secretion Effects 0.000 claims abstract description 7
- 239000013589 supplement Substances 0.000 claims abstract 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 30
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 30
- 239000011521 glass Substances 0.000 claims description 20
- 239000002689 soil Substances 0.000 claims description 20
- 210000004051 gastric juice Anatomy 0.000 claims description 19
- 238000012360 testing method Methods 0.000 claims description 16
- 229910052786 argon Inorganic materials 0.000 claims description 15
- 239000007789 gas Substances 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000000338 in vitro Methods 0.000 claims description 8
- 239000011229 interlayer Substances 0.000 claims description 6
- 238000003760 magnetic stirring Methods 0.000 claims description 6
- 239000000376 reactant Substances 0.000 claims description 4
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 3
- 102000001621 Mucoproteins Human genes 0.000 claims description 3
- 108010093825 Mucoproteins Proteins 0.000 claims description 3
- 102000057297 Pepsin A Human genes 0.000 claims description 3
- 108090000284 Pepsin A Proteins 0.000 claims description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 3
- 229960005091 chloramphenicol Drugs 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 claims description 3
- 239000010410 layer Substances 0.000 claims description 3
- 239000001630 malic acid Substances 0.000 claims description 3
- 235000011090 malic acid Nutrition 0.000 claims description 3
- 229940111202 pepsin Drugs 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 230000030136 gastric emptying Effects 0.000 abstract description 4
- 229910052785 arsenic Inorganic materials 0.000 description 6
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 239000000356 contaminant Substances 0.000 description 5
- 230000001079 digestive effect Effects 0.000 description 5
- 239000003344 environmental pollutant Substances 0.000 description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 5
- 231100000719 pollutant Toxicity 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000012502 risk assessment Methods 0.000 description 5
- 239000000741 silica gel Substances 0.000 description 5
- 229910002027 silica gel Inorganic materials 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000013019 agitation Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
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- 238000011109 contamination Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003802 soil pollutant Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000003723 Smelting Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 208000005368 osteomalacia Diseases 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
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- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Environmental & Geological Engineering (AREA)
- General Life Sciences & Earth Sciences (AREA)
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Abstract
The heavy metal biological for digesting feature the invention discloses a kind of simulation human stomach can be to system safety testing device and method, including the container for simulating peptic digest reaction；Feed block is reacted in peptic digest, connect with the container of simulation peptic digest reaction, for the feed liquid and gas required when supplement simulation human stomach's digestion into container, to simulate human stomach's gastric secretion and anaerobic digestion environment；Module is stirred, is located at the container bottom of simulation peptic digest reaction, for the feed liquid in stirring container, to simulate the wriggling of human stomach；Temperature control module is connect, for controlling the temperature of feed liquid in container, to simulate the temperature environment of human stomach with the container of simulation peptic digest reaction；Monitoring modular is located inside the container of simulation peptic digest reaction, for monitoring the temperature and pH value of feed liquid in container；Digestion reaction collection module, and the reservoir that reacts of simulation peptic digest, for simulating gastric emptying, and collection vessel it is digested after feed liquid.
Description
Technical field
The invention belongs to contaminated site environmental surveys and risk assessment technology field, are related to a kind of simulation human stomach digestion
The heavy metal-polluted soil Manual Suture test device and method of feature.
Background technique
Currently, soil pollution situation in China's allows of no optimist, especially in the cities and towns of some rapid economic developments, agglomeration of industries
Area, with the propulsion that urbanization process is accelerated and industrial upgrading adjusts, place soil contamination problem increasingly highlights, endangers people and occupy ring
Border safety and ecological safety.Heavy metal is the common spy in the industries carryover contamination such as lead storage battery, plating, chemical industry, smelting place
Pollutant is levied, there is easy accumulation, bio-refractory, hidden lag and irreversible characteristic, to the ecosystem and human health structure
At threat.Thus, the risk prevention in heavy metal pollution place is increasingly taken seriously.
Heavy metal in soil especially topsoil easily enters human body by the approach such as being orally ingested, directly to human body
Health threatens.For example, the cadmium of human body excess intake can cause osteomalacia, renal damage.Children are special due to its physiology and behavior
Point, the probability for being orally ingested heavy metal-polluted soil are bigger.For example, the lead in dust can enter in children's body and be accumulated through alimentary canal,
Generate a series of serious consequences such as slow body development, immunity of organisms reduction, failure of memory.As it can be seen that heavy metal-polluted soil is dirty
Object is contaminated after alimentary canal absorbs, and greatly harm can be generated to human body.
The form of place soil heavy metal contaminants determines its biological effectiveness and risk, the heavy metal-polluted soil of human body intake
In only portion forms can be bioavailable.However, China's contaminated site environmental survey and risk assessment are all with the total amount of pollutant
Based on, the heavy metal contaminants defaulted in soil can be digested completely.It is existing that " space enrironment technique for investigation is led
Then " (HJ25.1-2014), " contaminated site risk assessment technology directive/guide " (HJ 25.3-2014), " contaminated site soil remediation skill
Art directive/guide " (HJ 25.4-2014) etc. be not related to biological effectiveness content.The risk assessment for being currently based on the total amount of pollutant is difficult
With actual response health effect, often easily it is excessively high estimation place soil heavy metal contaminants risk, and then cause excessively repair,
Rehabilitation cost investment is excessively high, waste substantial contribution and human input, be unfavorable for contaminated site repairing and treating cause science and can
Sustainable development.
Directly very difficult using the biological effectiveness of body measurements pollutant, there is also the test periods for biological living test
The limitations such as long, costly.Testing in vitro method is mostly used at present, by the way that digestive juice can be modeled in measurement soil pollutant
Part, that is, Manual Suture of extraction predicts biological effectiveness, has short and easy regeneration of good reproducibility, test period etc.
Advantage can be used for the detection of batch samples.In the world, the in-vitro simulated test method such as PBET, IVG has had been established, it is substantially former
Manage it is similar, the composition of simulated digestive juice, pH, extraction time, in terms of have differences.In recent years, domestic scholars are also opened
Open up correlative study work, such as Zhong Maosheng has invented the heavy metal of simulation human consumption's feature and given property convertible test device
(CN103439476B), though more easily realizing heavy metal-polluted soil human body to the test of property, domestic and international existing research and
Related invention patent has been concerned only with the elements such as digestive juice composition, temperature environment, pH environment, also lacks and mainly disappears to human stomach etc.
The bionics fiber for changing organ there is no raw to soil pollutant to gastric digestions processes such as digestive juice secretion, stomach wriggling, gastric emptyings
Object is to the total system simulation of property and explanation, the considerations of especially shortage to the dynamic digestions process such as gastric secretion, gastric emptying, institute
The test result of acquisition is difficult to accurately reflect the true Manual Suture of heavy metal pollution of soil object, and reconditioning calculating is caused to be deposited
In deviation, become the probabilistic important sources of health risk assessment.
Summary of the invention
The purpose of the present invention is lack the bionical mould to gastric digestion process to property testing in vitro for heavy metal biological
Quasi- deficiency proposes that a kind of heavy metal biological of simulation human stomach digestion feature can be to system safety testing device, to testing soil
The Manual Suture of heavy metal contaminants.
The present invention also technical problems to be solved are to provide can using above-mentioned apparatus progress heavy metal pollution of soil object biology
To the testing in vitro method of property.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device, comprising:
For simulating the container of peptic digest reaction；
Feed block is reacted in peptic digest, connect with the container of simulation peptic digest reaction, for supplementing simulation into container
Human stomach feed liquid and gas required when digesting, to simulate human stomach's gastric secretion and anaerobic digestion environment；
Module is stirred, is located at the container bottom of simulation peptic digest reaction, for the feed liquid in stirring container, to simulate people
The wriggling of body stomach；
Temperature control module is connect, for controlling the temperature of feed liquid in container, with simulation with the container of simulation peptic digest reaction
The temperature environment of human stomach；
Monitoring modular is located inside the container of simulation peptic digest reaction, for monitoring the temperature and pH of feed liquid in container
Value；
Digestion reaction collection module, the reservoir reacted with simulation peptic digest, for simulating the evacuation procedure of stomach, and
Feed liquid after collection vessel is digested.
Specifically, peptic digest reaction feed block includes gastric juice receptacle, hydrochloric acid receptacle and argon tanks；It is described
Gastric juice receptacle conveys the simulate the gastric juice manually prepared into container by the first delivery pipe；The hydrochloric acid receptacle passes through second
Delivery pipe conveys hydrochloric acid solution into container, to simulate the pH environment of human stomach；The argon tanks by third delivery pipe to
Argon gas is passed through in container, to simulate the anaerobic environment of human stomach.
The container of the simulation peptic digest reaction is glass sandwich tank, and inside is reaction chamber, and interlayer is water-bath layer；It is described
Temperature control module is recirculated water bath, is connected with two water-baths inlet and outlet outside glass sandwich tank interlayer, to simulate human body
Stomach temperature environment.
The stirring module is magnetic stirring apparatus, is located at the container bottom of simulation peptic digest reaction, cooperates in container
Magnetic agitation rotor, for the feed liquid in stirring container, to simulate the wriggling of human stomach.
The monitoring modular includes thermometer, pH meter and parameter monitoring system；The thermometer and pH meter suspend respectively
Inside the container of simulation peptic digest reaction, and it is in contact with the feed liquid in container；The parameter monitoring system is located at simulation stomach
The external container of digestion reaction, and thermometer and pH meter are separately connected by data line, it collects and displays temperature meter and pH meter is surveyed
The temperature and pH value information measured.
The digestion reaction collection module is digestion reaction collector, anti-by the 4th delivery pipe and simulation peptic digest
The container bottom connection answered, the feed liquid for simulating the evacuation procedure of stomach, after collection vessel is digested.
First delivery pipe is equipped with the first constant flow pump, and artificial preparation is conveyed into container for controlling gastric juice receptacle
Simulate the gastric juice flow velocity；Second delivery pipe is equipped with the second constant flow pump, defeated into container for controlling hydrochloric acid receptacle
Send the flow velocity of hydrochloric acid solution；The third delivery pipe is equipped with gas flowmeter, and argon is passed through into container for controlling argon tanks
The speed of gas.
4th delivery pipe is equipped with third constant flow pump, for control container that simulation peptic digest is reacted to digestion reaction
The flow velocity of collector conveying feed liquid.
The testing in vitro method of heavy metal pollution of soil object Manual Suture is carried out using above-mentioned apparatus, including is walked as follows
It is rapid:
(1) collected pedotheque is dried at 60-80 DEG C, 0.25mm sieve is crossed after finely ground, 10g pedotheque is added
Inside the container for entering to simulate peptic digest reaction；
(2) prepare simulate the gastric juice: taking 1L deionized water, be added 0.3g pepsin, 2.0g mucoprotein, 3.0gNaCl,
1.5gKCl、0.35gCaCl2, 0.45g citric acid, 0.45g malic acid, pH value of solution is adjusted to 1.0-1.5 with the HCl of 0.5M, most
0.5ml chloramphenicol ethanol solution is added afterwards, is placed in spare in peptic digest reaction feed block；
(3) step (2) are prepared simulate the gastric juice to be added in the container that simulation peptic digest is reacted with the speed of 10mL/min, so
Feed block is reacted by peptic digest afterwards, argon gas is passed through into container with the speed of 1.0L/min；
(4) start temperature control module, so that the temperature of feed liquid is maintained at 37 DEG C in container；
(5) peptic digest is passed through when pH value is more than 1.5 by the temperature and pH value of feed liquid in monitoring module monitors container
Reaction feed block is passed through the HCl of 0.5M with the speed of 2mL/min into container, to control the pH value of feed liquid in container in 1.0-
Between 1.5；
(6) starting stirring module, is stirred with the speed of 150-200r/min；
(7) from when stirring 20min, start for the reactant in peptic digest reactor to be pumped into the speed of 4-5mL/min and disappear
Change in reaction collection module, until emptying；
(8) supernatant for extracting 20ml from digestion reaction collection module with 20ml syringe, is used after crossing 0.45 μm of film
Content of beary metal in ICP-MS filtrates tested, the Manual Suture as heavy metal in pedotheque.
The utility model has the advantages that
Compared with test method in existing organism, the advantage of the invention is that it is special to propose that a kind of simulation human stomach digests
The heavy metal biological of sign can give system safety testing device, pass through the Manual Suture of in-vitro simulated testing soil heavy metal contaminants, behaviour
Work is easy, easily controllable, economic cost is small, favorable repeatability.Compared with existing in vitro simulator and method, the present invention
It is operated according to the digestion reaction condition of human stomach, closer to human body true environment, including to stomach temperature and pH environment
Dynamic realtime regulation, provide anaerobic digestion environment, realize to gastric secretion, peristole, gastric emptying Whole Process Simulation,
To more comprehensively system, the digestion feature of human stomach has been simulated realistically, has been conducive to more accurately obtain a huge sum of money in soil
The Manual Suture test result for belonging to pollutant reflects that the environmental risk of heavy metal pollution of soil is horizontal.
Detailed description of the invention
The present invention is done with reference to the accompanying drawings and detailed description and is further illustrated, of the invention is above-mentioned
And/or otherwise advantage will become apparent.
Fig. 1 is that the heavy metal biological that simulation human stomach digests feature in embodiment can be to the structural representation of system safety testing device
Figure.
Each appended drawing reference respectively represents in figure: 1 gastric juice receptacle, 2 hydrochloric acid receptacles, 3 argon tanks, the first constant flow pump of 4a,
The second constant flow pump of 4b, 4c third constant flow pump, 5 gas flowmeters, 6 glass sandwich tanks, 7 thermometers, 8pH meter, 9 magnetic stirring apparatus,
10 digestion reaction collectors, 11 recirculated water baths, 12 parameter monitoring systems.
Specific embodiment
According to following embodiments, the present invention may be better understood.
Structure depicted in Figure of description, ratio, size etc., only to cooperate the revealed content of specification, with
Understand for those skilled in the art and read, be not intended to limit the invention enforceable qualifications, therefore does not have technically
Essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size can be generated not influencing the present invention
Under effect and the purpose that can reach, should all still it fall in the range of disclosed technology contents can cover.Meanwhile
Cited such as "upper", "lower", "front", "rear", " centre " term in this specification are merely convenient to being illustrated for narration, and
It is non-that to limit the scope of the invention, relativeness is altered or modified, under the content of no substantial changes in technology, when
Also it is considered as the enforceable scope of the present invention.
As shown in Figure 1, the heavy metal biological that simulation human stomach digests feature can include that gastric juice is deposited to system safety testing device
Put device 1, hydrochloric acid receptacle 2, argon tanks 3, the first constant flow pump 4a, the second constant flow pump 4b, third constant flow pump 4c, gas flowmeter 5,
Glass sandwich tank 6, thermometer 7, pH meter 8, magnetic stirring apparatus 9, digestion reaction collector 10, recirculated water bath 11 and parameter monitoring
System 12.It is reaction chamber inside glass sandwich tank 6, interlayer is water-bath layer, and recirculated water bath 11 passes through connecting silicone pipe and glass
Two water-baths inlet and outlet outside 6 interlayer of Sandwich pot are connected, and realize the simulation to stomach temperature environment；Gastric juice receptacle 1 passes through silicon
Glue delivery pipe is connect with the first constant flow pump 4a, and the other end of the first constant flow pump 4a protrudes into glass sandwich tank 6 by silica gel delivery pipe
The simulation to gastric secretion is realized in inside；Hydrochloric acid receptacle 2 is connect by silica gel delivery pipe with the second constant flow pump 4b, and second is permanent
The other end of stream pump 4b is protruded into inside glass sandwich tank 6 by silica gel delivery pipe, realizes the simulation to stomach pH environment；Argon tanks 3
It is connect by silica gel delivery pipe with gas flowmeter 5, the other end of gas flowmeter 5 protrudes into glass sandwich tank 6 by delivery pipe
Liquid level is hereinafter, realize the simulation to stomach anaerobic environment；Glass sandwich tank 6 is placed in 9 top of magnetic stirring apparatus, passes through magnetic agitation reality
The simulation now wriggled to peptic digest；Reactant outlet is arranged in 6 inner bottom of glass sandwich tank, permanent by glue delivery pipe and third
Stream pump 4c connection, the other end of third constant flow pump 4c are connect with digestion reaction collector 10 by silica gel delivery pipe, are realized to stomach
The simulation of evacuation procedure；Thermometer 7, pH meter 8 are suspended in inside peptic digest reactor 6, and are connected respectively with parameter monitoring system 12
It connects, collects and displays temperature meter 7 and pH meter 8 measure obtained temperature and pH value information.
Lead in the soil of place is left to certain industry using above-mentioned apparatus, the heavy metal biological of arsenic can be tested to property:
(1) it is left in industry and acquires pedotheque in place, sample is dried at 60-80 DEG C, cross 0.25mm after finely ground
10g pedotheque is added inside glass sandwich tank 6 sieve；
(2) prepare simulate the gastric juice, take 1L deionized water, be added 0.3g pepsin, 2.0g mucoprotein, 3.0gNaCl,
1.5gKCl、0.35gCaCl2, 0.45g citric acid, 0.45g malic acid, pH value of solution is adjusted to 1.0-1.5 with the HCl of 0.5M, is added
Entering 0.5ml chloramphenicol ethanol solution (0.5mg/ml ethyl alcohol) prevents microorganism from growing in digestive juice, and simulate the gastric juice is placed in stomach
In liquid receptacle 1；
(3) start the first constant flow pump 4a, be placed on the simulate the gastric juice in gastric juice receptacle 1 and be added with the speed of 10mL/min
Into glass sandwich tank 6；
(4) argon tanks 3 are opened, argon gas is led in glass sandwich tank 6 by gas flowmeter with the speed of 1.0L/min
Liquid in；
(5) start recirculated water bath 11, glass sandwich tank 6 is monitored by the thermometer 7 connecting with parameter monitoring system 12
The temperature of middle feed liquid keeps it in 37 DEG C；
(6) pH value that feed liquid in glass sandwich tank is monitored by the pH meter 8 connecting with parameter monitoring system 12, when pH value is super
When 1.5, then the HCl of 0.5M in hydrochloric acid receptacle 2 is added with the speed of 2mL/min by glass sandwich by the second constant flow pump 4b
In tank 6；
(7) start magnetic stirring apparatus 9, stirred with the speed of 150-200r/min；
(8) after stirring 20min, third constant flow pump 4c is opened, by the reactant in glass sandwich tank 6 with 4-5mL/min's
Speed is pumped into digestion reaction collector 10；
(9) supernatant for extracting 20ml from digestion reaction collector 10 with 20ml syringe, is used after crossing 0.45 μm of film
Lead, arsenic content in ICP-MS filtrates tested；
(10) three parts of pedotheques are successively tested by above-mentioned steps, the Manual Suture test result of lead, arsenic in each sample
As shown in table 1.
The Manual Suture of lead, arsenic in 1 pedotheque of table
As can be seen from the above table, the lead in pedotheque, arsenic pollution object cannot be absolutely in human stomach digestive organs
Middle dissolution, different sample room lead, arsenic pollution object Manual Suture size have differences.
The present invention provides a kind of heavy metal biologicals that simulation human stomach digests feature can give system safety testing device and method
Thinking and method, there are many method and the approach for implementing the technical solution, and the above is only preferred implementation of the invention
Mode, it is noted that for those skilled in the art, without departing from the principle of the present invention, also
Several improvements and modifications can be made, these modifications and embellishments should also be considered as the scope of protection of the present invention.It is unknown in the present embodiment
The available prior art of true each component part is realized.
Claims (9)
1. a kind of heavy metal biological that simulation human stomach digests feature can give system safety testing device characterized by comprising
For simulating the container of peptic digest reaction；
Feed block is reacted in peptic digest, connect with the container of simulation peptic digest reaction, for the supplement simulation human body into container
Required feed liquid and gas when gastric digestion, to simulate human stomach's gastric secretion and anaerobic digestion environment；
Module is stirred, is located at the container bottom of simulation peptic digest reaction, for the feed liquid in stirring container, to simulate human stomach
The wriggling in portion；
Temperature control module is connect, for controlling the temperature of feed liquid in container, to simulate human body with the container of simulation peptic digest reaction
The temperature environment of stomach；
Monitoring modular is located inside the container of simulation peptic digest reaction, for monitoring the temperature and pH value of feed liquid in container；
Digestion reaction collection module, the reservoir reacted with simulation peptic digest, for feed liquid of the collection vessel after digested.
2. the apparatus according to claim 1, which is characterized in that the peptic digest reaction feed block includes gastric juice storage
Device (1), hydrochloric acid receptacle (2) and argon tanks (3)；The gastric juice receptacle (1) conveys people into container by the first delivery pipe
The simulate the gastric juice that work is prepared；The hydrochloric acid receptacle (2) conveys hydrochloric acid solution into container by the second delivery pipe, to simulate people
The pH environment of body stomach；The argon tanks (3) are passed through argon gas into container by third delivery pipe, to simulate detesting for human stomach
Oxygen environment.
3. the apparatus according to claim 1, which is characterized in that the container of the simulation peptic digest reaction is glass sandwich
Tank (6), inside are reaction chamber, and interlayer is water-bath layer；The temperature control module is recirculated water bath (11), with glass sandwich tank
(6) two water-baths inlet and outlet outside interlayer are connected, to simulate human stomach's temperature environment.
4. the apparatus according to claim 1, which is characterized in that the stirring module is magnetic stirring apparatus (9), is located at
The container bottom for simulating peptic digest reaction, for the feed liquid in stirring container, to simulate the wriggling of human stomach.
5. the apparatus according to claim 1, which is characterized in that the monitoring modular includes thermometer (7), pH meter (8)
With parameter monitoring system (12)；The thermometer (7) and pH meter (8) are suspended in respectively inside the container of simulation peptic digest reaction,
And it is in contact with the feed liquid in container；The parameter monitoring system (12) is located at the external container of simulation peptic digest reaction, and leads to
It crosses data line and is separately connected thermometer (7) and pH meter (8), collect and displays temperature meter (7) and pH meter (8) measure obtained temperature
With pH value information.
6. the apparatus according to claim 1, which is characterized in that the digestion reaction collection module is digestion reaction collection
Device (10), by the 4th delivery pipe with simulate peptic digest react container bottom connection, for collection vessel it is digested after
Feed liquid.
7. the apparatus of claim 2, which is characterized in that first delivery pipe is equipped with the first constant flow pump (4a)；
Second delivery pipe is equipped with the second constant flow pump (4b)；The third delivery pipe is equipped with gas flowmeter (5).
8. device according to claim 6, which is characterized in that the 4th delivery pipe is equipped with third constant flow pump (4c).
9. claim 1 described device carries out the testing in vitro method of heavy metal pollution of soil object Manual Suture, feature exists
In including the following steps:
(1) collected pedotheque is dried at 60-80 DEG C, 0.25mm sieve is crossed after finely ground, mould is added in 10g pedotheque
Inside the container of quasi- peptic digest reaction；
(2) prepare simulate the gastric juice: taking 1L deionized water, be added 0.3g pepsin, 2.0g mucoprotein, 3.0gNaCl,
1.5gKCl、0.35gCaCl2, 0.45g citric acid, 0.45g malic acid, pH value of solution is adjusted to 1.0-1.5 with the HCl of 0.5M, most
0.5ml chloramphenicol ethanol solution is added afterwards, is placed in spare in peptic digest reaction feed block；
(3) step (2) are prepared simulate the gastric juice to be added in the container that simulation peptic digest is reacted with the speed of 10mL/min, is then led to
It crosses peptic digest reaction feed block and argon gas is passed through into container with the speed of 1.0L/min；
(4) start temperature control module, so that the temperature of feed liquid is maintained at 37 DEG C in container；
(5) it is reacted when pH value is more than 1.5 by peptic digest by the temperature and pH value of feed liquid in monitoring module monitors container
Feed block is passed through the HCl of 0.5M with the speed of 2mL/min into container, to control the pH value of feed liquid in container in 1.0-1.5
Between；
(6) starting stirring module, is stirred with the speed of 150-200r/min；
(7) from when stirring 20min, start the reactant in peptic digest reactor being pumped into digestion instead with the speed of 4-5mL/min
It answers in collection module, until emptying；
(8) supernatant for extracting 20ml from digestion reaction collection module with 20ml syringe, uses ICP-MS after crossing 0.45 μm of film
Content of beary metal in filtrates tested, the Manual Suture as heavy metal in pedotheque.
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