CN103439476A - Turnover testing device and method for heavy metal availability by stimulating human digestion characteristics - Google Patents
Turnover testing device and method for heavy metal availability by stimulating human digestion characteristics Download PDFInfo
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- CN103439476A CN103439476A CN2013103883399A CN201310388339A CN103439476A CN 103439476 A CN103439476 A CN 103439476A CN 2013103883399 A CN2013103883399 A CN 2013103883399A CN 201310388339 A CN201310388339 A CN 201310388339A CN 103439476 A CN103439476 A CN 103439476A
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Abstract
The invention provides a turnover testing device and method for heavy metal availability by stimulating human digestion characteristics. The method comprises the following steps of: (1) preprocessing a soil sample; (2) preparing simulated gastric fluid and intestinal fluid and heating; (3) simultaneously adding the preprocessed soil sample and the simulated gastric fluid into an extraction bottle and regulating the pH of the solution; (4) continuously overturning and oscillating; (5) at the end of oscillation, filtering with a filtering membrane, and analyzing the human availability of the heavy metal in a stomach stage by utilizing an ICP-MS (Inductively Coupled Plasma Mass Spectrometry); (6) after supplementing the fresh gastric fluid, adding the simulated gastric fluid, regulating the pH, and fixing the solution into an extraction chamber; (7) overturning and oscillating; (8) at the end of oscillation, filtering with the mixture to pass through the filtering membrane, and analyzing the human availability of the heavy metal in an intestine stomach stage by utilizing the ICP-MS. The testing method provided by the invention is simple in operation and large in testing capacity, requires few instruments, is capable of realizing automatic control and simultaneously testing 12 samples and can be widely used for testing the human availability of soil heavy metal in polluted sites.
Description
Technical field
The invention belongs to the field of Environment Protection of contaminated site health test, relate in particular to a kind of human intestines and stomach of simulation and digest the heavy metal in soil human body of feature to the convertible Test integration device and method of property.
Background technology
Because environmental practice in the production runes such as early stage smelting, metallic article (as electroplated, casting) processing and manufacturing, chromic salts is not in place, cause soil in these plant area of enterprise scopes to be subject to heavy metal pollution.Carry out place assessment discovery as Beijing moved to certain chemical plant, the As of plant area, Hg, Cr contaminated soil reach 3.1 ten thousand m
3.Dyestuff factory, Beijing soil is assessed to discovery, and As, Cr contaminated soil reach 5.2 ten thousand m
3.Discovery is assessed in discarded dyestuff factory, Wuhan, and the As contaminated soil reaches 18.4 ten thousand m
3.Affected by the blowdown of Zhuzhou Deng Di smelting enterprise, the By The Heavy Metal Contaminated Soil in whole Hunan Province nearly 13%, reach 28000 hm2.Plant area of former Changsha Chromates Plant soil is assessed to discovery, and the Cr contaminated soil that this plant area need to repair reaches 200,000 m
3.By in September, 2011,15 heavy metal pollution places have been determined by risk assessment in the Pengzhou, Sichuan.
For meeting the town site demand, these heavy metal industrial pollution place land used character often will change business or residential estate into.For guaranteeing this rear following use crowd's of class place redevelopment health, need be assessed and be taked corresponding risk control measure according to result heavy metal pollution degree in the soil of place.Wherein, formulate corresponding risk management based on human healthy risk assessment's result and control measure are accepted by environmental administration at different levels.At present when carrying out the heavy metal pollution of soil risk assessment, can enter the blood circulation of human body system fully and human body is worked the mischief after taking in human body due to the hypothesis heavy metal in soil with the soil per os, when calculating reconditioning, generally take the heavy metal in soil total amount as benchmark.But more and more research shows, even under extremely acid gastric juice condition, heavy metal also can not fully dissolve and enter in human gastric juice from soil.Therefore, it is very conservative that employing is assessed with the heavy metal in soil total amount result of being obtained, and causes huge sum of money contaminated soil excessively to be repaired, and wastes unnecessary reparation fund.
At present based on the heavy metal in soil total amount, assess the problem that causes result too conservative for overcoming, the most effective approach is the biological effectiveness (being in-vivo test) that adopts heavy metal in the animal toxicity test testing soil.But, this method often consuming time long, cost is higher, simultaneously because usining animal as tested donor, endures dispute to the fullest extent on ethics always.Because heavy metal in soil is absorbed the prerequisite that enters the blood circulation of human body system, it is the gastronintestinal system that it can desorb enters human body from soil, and large quantity research shows, be dissolved in the amount of pollutant in human intestines and stomach's system and correlativity preferably arranged, the exposed amount as human body that can guard with the measurer finally entered in blood circulation system.Therefore, take in-vitro simulated method of testing (being in-vitro test) that human stomach intestinal juice extracted pollutant in soil as extraction agent progressively replace in-vivo test that tradition is complicated for the heavy metal in soil human body can to the property test and by result for risk assessment.
Give the property method of testing as a kind of human body of can commercialization applying, when guaranteeing test result reliability, also need to possess proving installation and operating process simple, can carry out the batch samples test simultaneously and realize the advantages such as control automatically.Although domestic existing relevant patent of invention, proving installation is relatively complicated.As the test macro in the multi path artificial analogue gastronintestinal system assessment soil pollutant to human body validity method of Zhu Yong official rank invention comprises many polydisperse subsystems such as logical argon gas, stirring, the online titration of soda acid, pH on-line monitoring, complex operation, be difficult to control each sample in identical test environment.In a kind of dust/soil of the inventions such as Lu Gaosheng the extraction method of simulated gastric fluid of absorption of human body heavy metal only tested heavy metal in human gastric juice to property, do not test its in intestinal juice to property, result is difficult to objectively respond the actual human body of heavy metal to property.
Therefore; defect in view of existing related invention; the present invention proposes a kind of relatively simple, easy operating; and in simulated soil, the human body of heavy metal release behavior in human intestines and stomach's digestive system is to the convertible integrated test set of property and method comparatively objectively, and test result can be used in the efficiency assessment of health risk assessment and the risk control measure based on the protection health.
Summary of the invention
The objective of the invention is to propose a kind of heavy metal in soil human body to property reliable test result, proving installation and integrated test set and method simple to operate, that can carry out the batch samples test, can be to the property test, for heavy metal pollution place health risk assessment provides more objective human body exposure concentrations for the heavy metal-polluted soil human body.For realizing above target, technical solution of the present invention is to provide a kind of heavy metal of human body digestion feature of simulating to the convertible proving installation of property and method.
The convertible proving installation of giving property of heavy metal of simulation human body digestion feature, it is characterized in that including extraction chamber, centre bearing, the Teflon of 12 2L extraction flask with cover, 12 hole fixed panels, centre bearing and upset motor web member, upset motor support fixture, the upset motor, battery main switch, the rotating mode switch, the upset timer, rotating speed shows and adjusting apparatus, temperature shows and adjusting apparatus, temperature sensor, cooling fan, add Hot-air fan, supply socket, connect wire, the centre bearing securing member, the control device chamber, extraction chamber's upset gland bonnet, the detachable radiation panel in pulpit.
Further, extraction chamber and control device chamber are integrated in a table apparatus, add Hot-air fan, cooling fan, 12 hole control panels, temperature sensor and all are integrated in extraction chamber.
Further, temperature demonstration and adjusting apparatus, rotating speed demonstration and adjusting apparatus, rotating mode switch, upset timer, upset motor, the equal integrated installation of battery main switch are indoor in control device.
Further, 12 hole control panels and centre bearing are the integral type parts, have guaranteed the stability of device operation in the test process.
Further, centre bearing firmly is connected with the upset motor with upset motor web member by centre bearing, guarantee the to overturn high efficiency of motor and the stability of device operation.
Further, temperature sensor, add Hot-air fan, cooling fan, temperature and show with adjusting apparatus and be connected to form a temperature demonstration and automatic control system by being connected wire; At first, temperature sensor is converted to electric signal in real time by the temperature value of extraction chamber, by connecting wire, is passed to temperature demonstration and adjusting apparatus, and temperature shows that being converted into temperature reading with adjusting apparatus after receiving electric signal is shown; Simultaneously, temperature shows with adjusting apparatus and also 37 ℃ of the readings of this reading and setting compared, and when temperature reading<37 ℃, startup adds Hot-air fan; When temperature reading > 37 ℃ times, start cooling fan.
Further, upset motor, rotating speed demonstration are connected to form a upset electric machine control system with the upset timer by being connected wire with adjusting apparatus, battery main switch, rotating mode switch, and motor overturning pattern, flip-flop transition, rotating speed are shown and control.
Further, the convertible gland bonnet in extraction chamber is movable gland bonnet, in test, carries out in process, covers the convertible gland bonnet in extraction chamber extraction chamber is sealed, and prevents from extracting indoor temperature and reduces; After test finishes, open the convertible gland bonnet in extraction chamber, the convenient Teflon extraction flask with cover that takes out from 12 hole fixed panels carries out sample separation.
Further, having heat radiation seam on the detachable radiation panel in pulpit, the used heat produced in the motor operation course that can promote to overturn is dispersed into atmospheric environment, has ensured the operation of upset motor long-time continuous.
A kind of heavy metal human body of simulating human intestines and stomach's digestion characteristics provided by the invention, to the convertible method of testing of property, comprises the following steps:
Step 1: gather pedotheque, air-dry rear grinding and cross the 0.25mm sieve, the soil sample of collection cut size<0.25mm is as testing sample;
Step 2: configuration artificial simulation gastric juices and intestinal juice, wherein NaCl concentration 5504 mg/L, NaH in artificial simulation gastric juices
2pO4 concentration 533mg/L, KCl concentration 1649mg/L, CaCl
2concentration 799mg/L, NH
4cl concentration 612mg/L, concentration of glucose 1300mg/L; Glucuronic acid concentration 40mg/L, uric acid 170mg/L, aminoglucose hydrochloride 660mg/L, bovine serum albumin 1000mg/L, pepsin 1000mg/L, adjust the pH to 1.0 of artificial simulation gastric juices ± 0.2 with HCl; NaCl concentration 24542 mg/L, NaHCO3 concentration 22784mg/L, KCl concentration 1882mg/L, CaCl in artificial simulation intestinal juice
2concentration 422mg/L, MgCl
2concentration 100mg/L, uric acid 700mg/L, bovine serum albumin 2800mg/L, pancreatin 3000mg/L, lipase 500mg/L, bile prime 6000mg/L, adopt NaOH or HCl that the pH of artificial simulation intestinal juice is adjusted to 6.3 ± 0.2, the simulated gastric fluid of configuration and intestinal juice lid lid are sealed and are placed in water-bath be heated to 37 ℃;
Step 3: 20g soil sample and 450ml simulated gastric fluid are added in 2L Teflon extraction flask with cover successively, the rear manual overturning vibration of lid lid 1min, in the mensuration bottle, the pH of simulated gastric fluid, guarantee that its value is 1.0 ± 0.2, otherwise add HCl or NaOH adjustment pH to 1.2; So repeat, guarantee that continuous 3 pH readings are 1.0 ± 0.2; Cover tightly afterwards bottle cap sealing and extraction flask is fixed in the extraction flask fixed panel in extraction chamber;
Step 4: the temperature that extraction chamber is set is that 37 ℃, upset 60 rev/mins of motor speeds, flip-flop transition 1h, inverted pattern are forward, opens the upset motor control switch and begins turning extraction;
Step 5: continuous overturning is closed down the upset motor after extracting 1h, makes its quiescent setting 5min after taking off extraction flask, open bottle cover, and whether the pH of test simulation gastric juice is 1.0 ± 0.2; If so, with the 10ml glass syringe, draw the 10ml supernatant and cross 0.45 μ m filter membrane, with test the content of heavy metal in supernatant after 10ml glass sample bottle collection filtrate with ICP-MS, as the human body of peptic digest stage heavy metal in soil, give property; Otherwise sample cancels, the test of resampling;
Step 6: in extraction flask, add successively 10ml simulated gastric fluid and 1200ml simulated intestinal fluid, adjust pH to 6.8 with NaOH or HCl, cover tightly manual overturning vibration 1min after bottle cap, open the pH of simulated intestinal fluid in the bottle cap test bottle, guarantee that its value is 6.3 ± 0.2, adjust pH to 6.3 ± 0.2 otherwise add HCl or NaOH, so repeat, guarantee that continuous 3 pH readings are 6.3 ± 0.2; Cover tightly afterwards bottle cap sealing and extraction flask is fixed in the extraction flask fixed panel in extraction chamber;
Step 7: the temperature that extraction chamber is set is that 37 ℃, upset 60 rev/mins of motor speeds, flip-flop transition 4h, inverted pattern are forward, opens the upset motor control switch and begins turning extraction;
Step 8: continuous overturning is closed down the upset motor after extracting 4h, makes its quiescent setting 5min after taking off extraction flask, open bottle cover, and whether the pH of test simulation intestinal juice is 6.3 ± 0.2; If pH is 6.3 ± 0.2, with the 10ml glass syringe, draw the 10ml supernatant and cross 0.45 μ m filter membrane, test the content of heavy metal in supernatant after collecting filtrate and add 2ml HNO3 with 10ml glass sample bottle with ICP-MS, as the human body of intestinal digestion stage heavy metal in soil, give property; Otherwise sample cancels, the test of resampling.
Compared with prior art, the invention has the advantages that proving installation is simple, the extraction element that will overturn, heating system, temperature online demonstration and regulator control system, the demonstration of upset motor speed are integrated in a table apparatus with regulator control system.Simultaneously, the method for testing in the present invention is tentatively mixed rear adjustment System pH with soil sample and simulated intestinal fluid with soil sample in simulated gastric fluid respectively, makes it be stabilized in respectively 1.0 ± 0.2 and 6.3 ± 0.2.And, repeatedly test experiments shows, after adopting this mode to process, stomach extracts the stage and intestines extract the pH environmental energy of whole leaching process of stage respectively continually and steadily 1.0 ± 0.2 and 6.3 ± 0.2, avoided the adjustment of system pH in upset vibration test process, therefore, do not need to arrange pH on-line monitoring and acid base titration system, simplified operating process, reduced the complicacy of system.Simulation human intestines and stomach in the present invention digests the convertible integrated test set of the heavy metal-polluted soil of feature and adopts 1 upset motor to drive the vibration of overturning of 12 extraction flasks simultaneously, the vibration operating mode that can guarantee each extraction flask is consistent, makes the sample test result have comparability.
The accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the integrated test set structural representation that the present invention proposes.
Fig. 2 is the integrated test set vertical view that the present invention proposes.
Fig. 3 is the integrated test set front view that the present invention proposes.
Fig. 4 is the integrated test set left view that the present invention proposes.
Fig. 5 is the integrated test set right view that the present invention proposes.
Reference numeral: 1-extraction chamber, the 2-centre bearing, 3-2L Teflon extraction flask with cover, 4-12 hole fixed panel, 5-centre bearing and upset motor web member, 6-upset motor support fixture, the 7-motor that overturns, the 8-battery main switch, 9-rotating mode switch, the 10-timer that overturns, the 11-rotating speed shows and adjusting apparatus, the 12-temperature shows and adjusting apparatus, the 13-temperature sensor, the 14-cooling fan, 15-adds Hot-air fan, the 16-supply socket, 17-connects wire, 18-centre bearing securing member, 19-control device chamber, the convertible gland bonnet in 20-extraction chamber, the detachable radiation panel in 21-pulpit.
Embodiment
Below in conjunction with accompanying drawing, 1-5 is specifically described.
The convertible proving installation of giving property of heavy metal of this simulation human body digestion feature, include extraction chamber 1, centre bearing 2, the Teflon of 12 2L extraction flask 3 with cover, 12 hole fixed panels 4, centre bearing and upset motor web member 5, upset motor support fixture 6, upset motor 7, battery main switch 8, rotating mode switch 9, upset timer 10, rotating speed shows and adjusting apparatus 11, temperature shows and adjusting apparatus 12, temperature sensor 13, cooling fan 14, add Hot-air fan 15, supply socket 16, connect wire 17, centre bearing securing member 18, control device chamber 19, extraction chamber's upset gland bonnet 20, the detachable radiation panel 21 in pulpit.
Further, extraction chamber 1 is integrated in a table apparatus with control device chamber 19, adds Hot-air fan 15, cooling fan 14,12 hole control panels 4, temperature sensor 13 and all is integrated in extraction chamber 1.
Further, temperature shows with adjusting apparatus 12, rotating speed demonstration and adjusting apparatus 11, rotating mode switch 9, upset timer 10, upset motor 7, the equal integrated installation of battery main switch 8 in control device chamber 19.
Further, in 12 hole control panels 4, with centre bearing 2, be the integral type parts, guaranteed the stability of device operation in the test process.
Further, centre bearing 2 firmly is connected with upset motor 7 with upset motor web member 5 by centre bearing, guarantee the to overturn high efficiency of motor 7 and the stability of device operation.
Further, temperature sensor 13, add Hot-air fan 15, cooling fan 14, temperature and show with adjusting apparatus 11 and be connected to form a temperature demonstration and automatic control system by being connected wire 17; At first, temperature sensor 13 is converted to electric signal in real time by the temperature value of extraction chamber, by connecting wire 17, is passed to temperature demonstration and adjusting apparatus 11, and temperature shows that being converted into temperature reading with adjusting apparatus 11 after receiving electric signal is shown; Simultaneously, temperature shows with adjusting apparatus 11 and also 37 ℃ of the readings of this reading and setting compared, and when temperature reading<37 ℃, startup adds Hot-air fan 15; When temperature reading > 37 ℃ times, start cooling fan 14.
Further, upset motor 7, rotating speed demonstration are connected to form a upset electric machine control system with adjusting apparatus 11, battery main switch 8, rotating mode switch 9 and upset timer 10 by being connected wire 17, and motor overturning pattern, flip-flop transition, rotating speed are shown and control.
Further, the convertible gland bonnet 20 in extraction chamber is movable gland bonnets, in test, carries out in process, covers 20 pairs of extraction chambers 1 of convertible gland bonnet, extraction chamber and is sealed, and prevents that the interior temperature in extraction chamber 1 from reducing; After test finishes, open the convertible gland bonnet 20 in extraction chamber, the convenient Teflon extraction flask 3 with cover that takes out from 12 hole fixed panels 4 carries out sample separation.
Further, having heat radiation seam on the detachable radiation panel in pulpit (21), the used heat produced in motor (7) operational process that can promote to overturn is dispersed into atmospheric environment, has ensured the operation of upset motor (7) long-time continuous.
Concrete method of testing step of the present invention is as follows:
(1) sample collection and pre-service
Gather 3 representativenesses of being polluted by As, Cd, Pb and pollute soil sample from the heavy metal pollution place, cross the 0.25mm sieve after natural air drying, the sample of collection cut size≤0.25mm is as specimen.
(2) configuration of artificial simulation gastric juices and intestinal juice
Artificial simulation gastric juices: get the 1350ml deionized water, add successively NaCl 7430.4mg, NaH
2pO
4719.55mg, KCl 2226.15mg, CaCl
21078.65mg, NH
4cl 826.2mg/L, glucose 1755mg; Glucuronic acid 54mg, uric acid 229.5mg, aminoglucose hydrochloride 891mg, bovine serum albumin 1350mg, pepsin 1350mg, with salt acid for adjusting pH to 1.0 ± 0.2;
Artificial simulation intestinal juice: get the 3600ml deionized water, add successively NaCl 883512mg, NaHCO
3820224mg, KCl 6775.2mg, CaCl
2151.2mg, MgCl
2360mg/L, uric acid 2520mg, bovine serum albumin 10080mg, pancreatin 10800mg, lipase 1800mg, bile prime 21600mg, adopt NaOH or HCl that the pH of artificial simulation intestinal juice is adjusted to 6.3 ± 0.2;
Before test experiments starts, the artificial simulation gastric juices that configuration is completed and intestinal juice are positioned in water-bath and are heated to 37 ℃.
(3) giving property of test peptic digest stage heavy metal human body
Get respectively the sample of 20g particle diameter≤0.25mm and put into 3 2L Teflon extraction flasks 3, the artificial simulation gastric juices that adds respectively 450ml to configure, cover after bottle cap in manual overturning vibration 1min after in test bottle system pH and be adjusted to 1.0 ± 0.2 with HCl.To being fixed in 12 hole fixed panels 4 in extraction chamber 1 after the sealing of extraction flask lid, cover the convertible gland bonnet 20 in extraction chamber.
Open the total power switch 8 of integrated test set, the upset motor speed is set is 55 turn/min, extract indoor temperature is that 37 ℃, extraction time are after 1h, inverted pattern are forward, starts upset motor 7 and extracts continuously.
After reaching and setting extraction time, upset motor 7 stops automatically.Open the convertible gland bonnet 20 in extraction chamber, after the static 5min of taking-up extraction flask, the pH of the interior simulated gastric fluid of test bottle is respectively 1.05,1.02,1.12, all in 1.0 ± 0.2 scopes.Draw after the 10ml supernatant is crossed 0.45 μ m filter membrane and be collected in sample bottle with clean 10ml glass syringe respectively, the property of giving by As, Cd in ICP-MS test supernatant, Pb concentration as the peptic digest stage.
(4) giving property of test intestinal digestion stage heavy metal human body
At first, supplement in the extraction flask 3 in 10ml simulated gastric fluid to 3 step (3) respectively.Afterwards, add respectively the 1200ml simulated intestinal fluid, cover after bottle cap after manual overturning vibration 1min in test bottle the pH of system and be adjusted to 6.3 ± 0.2 with NaOH or HCl.To being fixed in after extraction flask lid sealing in extraction chamber in 1 12 hole fixed panels 4, cover the convertible gland bonnet 20 in extraction chamber.
Open the total power switch 8 of integrated test set, the upset motor speed is set is 55 turn/min, the interior temperature in extraction chamber 1 and be 37 ℃, extraction time is after 4h, inverted pattern are forward, starts upset motor 7 and extracts continuously.
After reaching and setting extraction time, upset motor 7 stops automatically.Open extraction chamber's gland bonnet, after the static 5min of taking-up extraction flask, the pH of the interior simulated gastric fluid of test bottle is respectively 6.25,6.41,6.38, all in 6.3 ± 0.2 scopes.Draw after the 10ml supernatant is crossed 0.45 μ m filter membrane and be collected in sample bottle with clean 10ml glass syringe respectively, add 1ml HNO
3, the property of giving by As, Cd in ICP-MS test supernatant, Pb concentration as the intestinal digestion stage.
(5) in three of embodiment soil sample samples, the stomach of As, Cd, Pb, the human body in intestines stage are given the property test result respectively as shown in Table 1 and Table 2.
Claims (10)
1. a heavy metal of simulating human body digestion feature is to the convertible proving installation of property, it is characterized in that including extraction chamber (1), centre bearing (2), the Teflon of 12 2L extraction flask with cover (3), 12 hole fixed panels (4), centre bearing and upset motor web member (5), upset motor support fixture (6), upset motor (7), battery main switch (8), rotating mode switch (9), upset timer (10), rotating speed shows and adjusting apparatus (11), temperature shows and adjusting apparatus (12), temperature sensor (13), cooling fan (14), add Hot-air fan (15), supply socket (16), connect wire (17), centre bearing securing member (18), control device chamber (19), extraction chamber's upset gland bonnet (20), the detachable radiation panel in pulpit (21).
2. the heavy metal of simulation human body digestion feature according to claim 1 is to the convertible proving installation of property, it is characterized in that extraction chamber (1) and control device chamber (19) are integrated in a table apparatus, add Hot-air fan (15), cooling fan (14), 12 hole control panels (4), temperature sensor (13) and all be integrated in extraction chamber (1).
3. the convertible proving installation of giving property of heavy metal of simulation human body digestion feature according to claim 1, is characterized in that temperature shows and adjusting apparatus (12), rotating speed show with adjusting apparatus (11), rotating mode switch (9), upset timer (10), upset motor (7), the equal integrated installation of battery main switch (8) in control device chamber (19).
4. the convertible proving installation of giving property of heavy metal of simulation human body digestion feature according to claim 1, is characterized in that 12 hole control panels (4) and centre bearing (2) are the integral type parts, guaranteed the stability of device operation in the test process.
5. the heavy metal of simulation human body digestion feature according to claim 1 is to the convertible proving installation of property, it is characterized in that centre bearing (2) firmly is connected with upset motor (7) with upset motor web member (5) by centre bearing, guarantee the to overturn high efficiency of motor (7) and the stability of device operation.
6. the heavy metal of simulation human body according to claim 1 digestion feature gives property convertible proving installation, it is characterized in that temperature sensor (13), adds Hot-air fan (15), cooling fan (14), temperature shows with adjusting apparatus (11), by being connected wire (17), is connected to form a temperature demonstration and automatic control system; At first, temperature sensor (13) is converted to electric signal in real time by the temperature value of extraction chamber, be passed to temperature demonstration and adjusting apparatus (11) by connecting wire (17), temperature shows that being converted into temperature reading with adjusting apparatus (11) after receiving electric signal is shown; Simultaneously, temperature shows with adjusting apparatus (11) and also 37 ℃ of the readings of this reading and setting compared, and when temperature reading<37 ℃, startup adds Hot-air fan (15); When temperature reading > 37 ℃ times, start cooling fan (14).
7. the heavy metal of simulation human body digestion feature according to claim 1 is to the convertible proving installation of property, it is characterized in that overturning motor (7), rotating speed shows with adjusting apparatus (11), battery main switch (8), rotating mode switch (9) and is connected to form the electric machine control system that overturns with upset timer (10) by being connected wire (17), and motor overturning pattern, flip-flop transition, rotating speed are shown and control.
8. the heavy metal of simulation human body digestion feature according to claim 1 is to the convertible proving installation of property, it is characterized in that the convertible gland bonnet in extraction chamber (20) is movable gland bonnet, in test, carry out in process, cover the convertible gland bonnet in extraction chamber (20) extraction chamber (1) is sealed, prevent that the interior temperature in extraction chamber (1) from reducing; After test finishes, open the convertible gland bonnet in extraction chamber (20), the convenient Teflon extraction flask with cover (3) that takes out from 12 hole fixed panels (4) carries out sample separation.
9. the heavy metal of simulation human body digestion feature according to claim 1 is to the convertible proving installation of property, it is characterized in that on the detachable radiation panel in pulpit (21) having the heat radiation seam, the used heat produced that can promote to overturn in motor (7) operational process is dispersed into atmospheric environment, has ensured the operation of upset motor (7) long-time continuous.
10. a heavy metal of simulating human body digestion feature is to the convertible method of testing of property, and its step comprises:
Step 1: gather pedotheque, air-dry rear grinding and cross the 0.25mm sieve, the soil sample of collection cut size<0.25mm is as testing sample;
Step 2: configuration artificial simulation gastric juices and intestinal juice, wherein NaCl concentration 5504 mg/L, NaH in artificial simulation gastric juices
2pO
4concentration 533mg/L, KCl concentration 1649mg/L, CaCl
2concentration 799mg/L, NH
4cl concentration 612mg/L, concentration of glucose 1300mg/L; Glucuronic acid concentration 40mg/L, uric acid 170mg/L, aminoglucose hydrochloride 660mg/L, bovine serum albumin 1000mg/L, pepsin 1000mg/L, adjust the pH to 1.0 of artificial simulation gastric juices ± 0.2 with HCl; NaCl concentration 24542 mg/L, NaHCO in artificial simulation intestinal juice
3concentration 22784mg/L, KCl concentration 1882mg/L, CaCl
2concentration 422mg/L, MgCl
2concentration 100mg/L, uric acid 700mg/L, bovine serum albumin 2800mg/L, pancreatin 3000mg/L, lipase 500mg/L, bile prime 6000mg/L, adopt NaOH or HCl that the pH of artificial simulation intestinal juice is adjusted to 6.3 ± 0.2, the simulated gastric fluid of configuration and intestinal juice lid lid are sealed and are placed in water-bath be heated to 37 ℃;
Step 3: 20g soil sample and 450ml simulated gastric fluid are added in 2L Teflon extraction flask with cover successively, the rear manual overturning vibration of lid lid 1min, in the mensuration bottle, the pH of simulated gastric fluid, guarantee that its value is 1.0 ± 0.2, otherwise add HCl or NaOH adjustment pH to 1.2; So repeat, guarantee that continuous 3 pH readings are 1.0 ± 0.2; Cover tightly afterwards bottle cap sealing and extraction flask is fixed in the extraction flask fixed panel in extraction chamber;
Step 4: the temperature that extraction chamber is set is that 37 ℃, upset 60 rev/mins of motor speeds, flip-flop transition 1h, inverted pattern are forward, opens the upset motor control switch and begins turning extraction;
Step 5: continuous overturning is closed down the upset motor after extracting 1h, makes its quiescent setting 5min after taking off extraction flask, open bottle cover, and whether the pH of test simulation gastric juice is 1.0 ± 0.2; If so, with the 10ml glass syringe, draw the 10ml supernatant and cross 0.45 μ m filter membrane, with test the content of heavy metal in supernatant after 10ml glass sample bottle collection filtrate with ICP-MS, as the human body of peptic digest stage heavy metal in soil, give property; Otherwise sample cancels, the test of resampling;
Step 6: in extraction flask, add successively 10ml simulated gastric fluid and 1200ml simulated intestinal fluid, adjust pH to 6.8 with NaOH or HCl, cover tightly manual overturning vibration 1min after bottle cap, open the pH of simulated intestinal fluid in the bottle cap test bottle, guarantee that its value is 6.3 ± 0.2, adjust pH to 6.3 ± 0.2 otherwise add HCl or NaOH, so repeat, guarantee that continuous 3 pH readings are 6.3 ± 0.2; Cover tightly afterwards bottle cap sealing and extraction flask is fixed in the extraction flask fixed panel in extraction chamber;
Step 7: the temperature that extraction chamber is set is that 37 ℃, upset 60 rev/mins of motor speeds, flip-flop transition 4h, inverted pattern are forward, opens the upset motor control switch and begins turning extraction;
Step 8: continuous overturning is closed down the upset motor after extracting 4h, makes its quiescent setting 5min after taking off extraction flask, open bottle cover, and whether the pH of test simulation intestinal juice is 6.3 ± 0.2; If pH is 6.3 ± 0.2, with the 10ml glass syringe, draw the 10ml supernatant and cross 0.45 μ m filter membrane, collect filtrate and add 2ml HNO with 10ml glass sample bottle
3the rear content with heavy metal in ICP-MS test supernatant, as the giving property of human body of intestinal digestion stage heavy metal in soil; Otherwise sample cancels, the test of resampling.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531986A (en) * | 2015-01-05 | 2015-04-22 | 攀钢集团研究院有限公司 | Experimental facility for hydrometallurgy pressure leaching experiment |
| CN109975524A (en) * | 2019-05-08 | 2019-07-05 | 江苏省环境科学研究院 | It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method |
| CN111650262A (en) * | 2020-04-29 | 2020-09-11 | 北京市环境保护科学研究院 | Biological availability test method for hexavalent chromium respiratory pathway in soil |
| WO2022140868A1 (en) * | 2020-12-30 | 2022-07-07 | Universidad Tecnológica Metropolitana | Portable modular gastrointestinal simulator system that quantifies bioaccessibility of metals in a sample remotely and in real time, and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002214199A (en) * | 2001-01-17 | 2002-07-31 | Sumitomo Metal Mining Co Ltd | Determination method of soil contamination area |
| CN1719250A (en) * | 2004-07-06 | 2006-01-11 | 中国科学院生态环境研究中心 | Multi path artificial analogue gastronintestinal system assessment soil pollutant to human body validity method |
| CN101726436A (en) * | 2009-12-24 | 2010-06-09 | 浙江大学 | Extraction method of simulated gastric fluid of human body absorptivity heavy metals in dust/soil |
| CN202658158U (en) * | 2012-05-14 | 2013-01-09 | 中国科学院城市环境研究所 | Intelligent analog control device for organism gastrointestinal tract digestive system |
-
2013
- 2013-08-30 CN CN201310388339.9A patent/CN103439476B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002214199A (en) * | 2001-01-17 | 2002-07-31 | Sumitomo Metal Mining Co Ltd | Determination method of soil contamination area |
| CN1719250A (en) * | 2004-07-06 | 2006-01-11 | 中国科学院生态环境研究中心 | Multi path artificial analogue gastronintestinal system assessment soil pollutant to human body validity method |
| CN101726436A (en) * | 2009-12-24 | 2010-06-09 | 浙江大学 | Extraction method of simulated gastric fluid of human body absorptivity heavy metals in dust/soil |
| CN202658158U (en) * | 2012-05-14 | 2013-01-09 | 中国科学院城市环境研究所 | Intelligent analog control device for organism gastrointestinal tract digestive system |
Non-Patent Citations (5)
| Title |
|---|
| JIANZHOU HE 等: "Bioaccessibility of Vanadium from Soil and Mineral Measured by in vitro Model", 《SOIL AND FERTILIZER》 * |
| US EPA: "《USEPA9200.1-86》", 31 May 2008 * |
| 付瑾等: "In vitro 系统评价胃肠液pH及土液比对铅、镉、砷生物可给性的影响", 《农业环境科学学报》 * |
| 唐翔宇等: "In Vitro 法评估铅污染土壤对人体的生物有效性", 《环境化学》 * |
| 崔岩山等: "利用3 种in vitro 方法比较研究污染土壤中铅、砷生物可给性", 《农业环境科学学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531986A (en) * | 2015-01-05 | 2015-04-22 | 攀钢集团研究院有限公司 | Experimental facility for hydrometallurgy pressure leaching experiment |
| CN109975524A (en) * | 2019-05-08 | 2019-07-05 | 江苏省环境科学研究院 | It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method |
| CN111650262A (en) * | 2020-04-29 | 2020-09-11 | 北京市环境保护科学研究院 | Biological availability test method for hexavalent chromium respiratory pathway in soil |
| WO2021218521A1 (en) * | 2020-04-29 | 2021-11-04 | 北京市环境保护科学研究院 | Method for testing bioavailability of hexavalent chromium in soil by respiration pathway |
| WO2022140868A1 (en) * | 2020-12-30 | 2022-07-07 | Universidad Tecnológica Metropolitana | Portable modular gastrointestinal simulator system that quantifies bioaccessibility of metals in a sample remotely and in real time, and method |
| EP4184165A4 (en) * | 2020-12-30 | 2024-04-17 | Univ Tecnologica Metropolitana | Portable modular gastrointestinal simulator system that quantifies bioaccessibility of metals in a sample remotely and in real time, and method |
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