CN1719250A - Multi path artificial analogue gastronintestinal system assessment soil pollutant to human body validity method - Google Patents
Multi path artificial analogue gastronintestinal system assessment soil pollutant to human body validity method Download PDFInfo
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Abstract
A kind of multi path artificial analogue gastronintestinal system assessment of the present invention soil pollutant to human body validity method belongs to environmental technology field, relates to the human health risk evaluation of heavy metal pollution of soil, and the experimental system and the method for heavy metal-polluted soil biological effectiveness mensuration.The present invention includes the following step: the foundation of a. multi path artificial analogue gastronintestinal system; B. the collection of pedotheque and processing; C. prepare artificial digestion liquid; D. soil sample and artificial digestion liquid are added the multi path artificial analogue gastronintestinal system; E. in control certain reaction condition,, in the reaction vessel of the artificial gastrointestinal reaction device of system, gather a certain amount of water sample through behind the certain hour; F. after filtering, measure the soluble state heavy metal contaminants content in the water sample.System and method of the present invention can be used in a large number and examination contaminated soil apace, measures the biological effectiveness that the pollutant per os is taken in human body, estimates the validity of soil pollution to human health risk and soil remediation measure.
Description
Technical field
The invention belongs to environmental technology field, relate to the human health risk evaluation of soil pollution, particularly relate to the foundation of the experimental system and the method for soil pollution risk assessment.
Background technology
The research of existing heavy metal pollution of soil is to be point of penetration with soil-botanical system mostly, the research heavy metal enters human body by the food chain approach total amount.Undoubtedly, food chain is that heavy metal enters a very important source in the human body.In China, people have been fully recognized that heavy metal contained in growing crop on the contaminated soil or the agricultural product is too high, the harm that may cause health.Various reclamation activitiess by contaminated land, comprise physics (as soil moved in to improve the original etc.), chemistry (adding soil additive and passivator etc.) and biological method (as plant extract etc.), or the soil utilizes the change and the adjustment of mode, and the heavy metal that enters human body via food chain reduces in a large number.Therefore, under the situation that the heavy metal intake of food chain approach progressively is effectively controlled, take in the important source that (the directly contact activity of hand-mouth) can become human body absorption heavy metal under many circumstances through oral area unintentionally.Particularly for children, because its shortage health consciousness and outdoor sports time are longer, health consciousness for want of, the soil of heavy metal pollution can be bonded on hand, directly enter alimentary canal when being at play and taking food, thereby cause the main path of the human body exposure of per os absorption can becoming heavy-metal contaminated soil.Generally speaking, children's per os unintentionally the soil intake can reach the 50-200 mg/day.Have report to show, children by have a mind to the per os absorption the soil amount in addition may be up to 60 gram/skies.Children are just in growth and development stage, and are more more responsive than the adult to heavy metal pollution.Therefore, be necessary very much to take effective ways, assess the validity of the human body per os absorption of heavy-metal contaminated soil, for critical value or the clean environment desired value of determining heavy metal-polluted soil provides reliable foundation.
Heavy metal in the assessment soil has two kinds of methods, i.e. zoopery and experiment in vitro usually to the biological effectiveness of human body.Traditional animal oral toxicity result of experiment is commonly referred to be quite reliable, but the application of this method, is subjected to the restriction of its relatively long experimental period and higher experimental expenses.Just because of this, the assessment of the extraction of more quick and economic heavy metal-polluted soil validity then is suggested.Many researchs all show, based on physiological experiment in vitro, show good correlativity with zooperal result.Over past ten years, this method is constantly improved and is perfect, and its range of application is widened day by day.
Under the multi-point and wide-ranging background of China's heavy metal pollution of soil, assess the validity of the toxic heavy metal in the soil that the human body per os takes in reliably, become improve China people health quality one of very important focal issue.The appearance of experiment in vitro and development are for this assessment provides a kind of economy, quick and effective means.On the one hand, it is comparatively serious to screen out pollution level by in vitro method screening, behind the soil that may be detrimental to health, can carry out toxicologic further investigation in conjunction with zoopery.On the other hand, can use the validity of experiment in vitro quick test soil remediation measure.In addition, more existing researchers set about using in vitro method research heavy metal-polluted soil and enter the contingent morphological feature of alimentary canal and different shape heavy metal and stride film and absorb the qualitative and quantitative rule of moving, and can whereby on form transformation mechanism, estimate the feasibility and the validity of reclamation activities.Therefore, can expect, experiment in vitro has quite wide application prospect in the human health risk evaluation of China's heavy-metal contaminated soil and the recruitment evaluation research of reclamation activities, and then for formulating soil quality standard or the cleanliness standard that ensures health more all sidedly, and the reclamation activities decision-making, reliable scientific basis is provided.
The result's of experiment in vitro reliability depends primarily on the gastro-intestinal digestion system that experimental system is to what extent simulated human body.Under this background, the present invention proposes using artificial simulation stomach and intestine simulation system, as a kind of more reliable extracorporeal experiment system, on reaction conditions, farthest simulate the actual environment of human body gastro-intestinal digestion system, the validity that fast measuring heavy metal pollution of soil thing per os is taken in, and then assess the health risk of heavy metal pollution of soil, and can be used for the evaluation of the validity of soil pollution reclamation activities to human body.
Summary of the invention
The objective of the invention is the rapid screening heavy-metal contaminated soil, clear and definite heavy metal-polluted soil is taken in validity to the per os of human body, be used for the human health risk of heavy metal pollution of soil and the evaluation of soil pollution reclamation activities validity, provide reliable basis for formulating reasonable and economic heavy metal-polluted soil cleaning complex.
In order to achieve the above object, technical solution of the present invention provides a cover multi path artificial analogue gastronintestinal system assessment soil pollutant to human body validity method, comprises the following steps:
A, set up the multi path artificial analogue gastronintestinal system;
The collection of b, pedotheque and processing;
C, preparation artificial digestion liquid;
D, soil sample and artificial digestion liquid are added the multi path artificial analogue gastronintestinal system;
E, in control certain reaction condition, through behind the certain hour, in the reaction vessel of the artificial gastrointestinal reaction device of system, gather a certain amount of water sample;
After f, the filtration, measure the pollutant load in the water sample.
Described method is set up the multi path artificial analogue gastronintestinal system described in its a step, comprises argon gas supply subsystem (argon gas steel cylinder, separation panel and gas meter), multi path artificial stomach and intestine analogue reactor (8 reactors) and automatic acid alkalimetric titration instrument; Wherein, argon gas supply subsystem links to each other with multi path artificial stomach and intestine analogue reactor, 4/5 of the reaction vessel of artificial stomach and intestine analogue reactor places water bath, the probe of automatic acid alkalimetric titration instrument places the interior reactant liquor of reaction vessel of artificial stomach and intestine analogue reactor, and its liquid feeding head places reactant liquor top 2～3cm.。
Described method, its described argon gas supply subsystem comprises the argon gas steel cylinder, separation panel and gas meter, the argon gas steel cylinder is communicated with separation panel by reduction valve, is provided with gas meter in a plurality of passages of separation panel.
Described method, its described artificial stomach and intestine analogue reactor, comprise control panel, glass reaction container and by the stirrer of poly-tetrafluoro bag quilt, a plurality of channel connections of control panel and separation panel, reaction vessel and stirrer and control panel are connected, and the lower end of passage and stirrer place the bottom in the reaction vessel.
Described method, the probe of its described automatic acid alkalimetric titration instrument place the interior reactant liquor of reaction vessel of artificial stomach and intestine analogue reactor, and its liquid feeding head places reactant liquor top 2～3cm.
Described method, its described reaction vessel is 6～8.
Described method; the collection and the processing of pedotheque described in its b step; since this method is paid close attention to be crowd (particularly children) out of doors in the activity contaminated soil of hand-mouth contact activity per os absorption to the health risk of human body; in addition; usually＜and the soil particle of 0.25mm can stick on hand, and this method requires to gather top layer 0～5cm soil, and is air-dry; cross 0.25mm sieve, get＜the 0.25mm soil sample carries out next step analysis.
Described method, preparation artificial digestion liquid described in its c step, its method is: 5 liters of every batch sample preparation simulated gastric fluid, include 0.13～0.17M NaCl, add citric acid 2.0～3.0 grams, malic acid 2.0～3.0 grams, 2.0～2.2 milliliters of lactic acid, 2.0～3.0 milliliters of glacial acetic acids and pepsin 6.0～6.5 grams, the pH value is transferred to 1.3～1.7 with 12M HCl; Standby.
Described method, it in step, is soil to be measured 6 grams that add 550～650 milliliters artificial digestion liquid respectively and cross 0.25 mm sieve in each reaction vessel at d.
Described method, it in step, places 37 ℃ water-baths with reaction vessel at e, speed with 0.8～1.2 liter/minute in artificial digestion liquid feeds argon gas, to create anaerobic environment, with 80～120 rev/mins of stirrings, in the beginning stomach stage, adopt automatic acid alkalimetric titration instrument with HCl or NaHCO
3Control pH value is reacted after 1 hour 1.3～1.7, draws 20 milliliters of simulation stomach reactant liquors with syringe; Then, in each reaction vessel, add NaHCO respectively
3Powder is regulated pH value to 6.8～7.2, adds 0.34～0.38 gram trypsase and 1.1～1.3 gram cholate again, in the beginning small intestine stage, adopts automatic acid alkalimetric titration instrument with HCl or NaHCO
3Control pH value after 4 hours, is drawn 20 milliliter reactant liquors with syringe 6.8～7.2.
Described method, it places centrifuge tube with simulation stomach or the small intestine reactant liquor of being gathered in f step, with 10000 rev/mins centrifugal 10 minutes, 0.45 micron filtration adopts atomic absorption method to analyze pollutant load in the filtrate.
The inventive method the results showed, the good reproducibility of this method, and the per os that can record heavy metal-polluted soil is apace taken in validity, can be used for the rapid screening contaminated soil and estimates the validity of soil remediation measure.
Description of drawings
Fig. 1 proposes to be used to measure the multi path artificial analogue gastronintestinal system that the heavy metal-polluted soil per os is taken in validity through the present invention.
Embodiment
In the risk of assessment heavy-metal contaminated soil to health, adopt multi path artificial analogue gastronintestinal system proposed by the invention and experiment in vitro method, after measuring the absorption of contaminated soil per os, the content of the soluble state heavy metal in manual simulation's digestive juice in the stage at stomach and small intestine, can be used for the rapid screening contaminated soil, provide foundation for formulating corresponding soil cleaning desired value, and can be used for the evaluation of soil remediation measure validity.
One, the foundation of multi path artificial analogue gastronintestinal system
The present invention makes multi path artificial analogue gastronintestinal system (as Fig. 1) by oneself on the basis of original simple intellectual drug dissolution test instrument, simulated human intestines and stomach's digestion process more truly.This system is by argon supply system (comprising argon gas steel cylinder, reduction valve, separation panel and flowmeter), and water bath (temperature-controlled precision ± 0.3 ℃, comprise 8 glass reaction containers and by the stirrer of poly-tetrafluoro bag quilt) and automatic acid alkalimetric titration instrument are formed.
Two, the collection of pedotheque to be measured and preparation
Gather top layer, contaminated area 0～5cm soil, air-dry, cross the 0.25mm sieve, get＜the 0.25mm soil sample carries out next step analysis.
Three, measure the validity that the pedotheque per os is taken in
In the reaction vessel of the multi path artificial analogue gastronintestinal system that the present invention designs, add 6 pedotheque and 550～650 milliliters of manual simulation's digestive juices that restrained 0.25 mm sieve and (contain 0.13～0.17M NaCl, 0.04～0.06% citric acid, 0.04～0.06% malic acid, 0.04～0.044% lactic acid, 0.04～0.06% glacial acetic acid and 0.12～0.172% proteinase, pH 1.3～1.7), feed argon gas (0.8～1.2 liter/minute), 80～120 rev/mins of stirrings, the beginning stomach dummy run phase, adopt automatic acid alkalimetric titration instrument with HCl or NaHCO
3Control pH value is drawn 20 milliliters of stomach simulation reaction liquid 1.3～1.7 after 1 hour; In each reaction vessel, add NaHCO
3Powder is regulated pH to 6.8～7.2, adds 0.34～0.38 gram trypsase and 1.1～1.3 gram cholate again, and the beginning small intestine dummy run phase, titration control is with HCl or NaHCO automatically
3At pH 6.8～7.2, draw 20 milliliters of small intestine simulation reaction liquid after 4 hours.
With the stomach gathered and small intestine simulation reaction liquid 10000 rev/mins centrifugal, get supernatant, the content of beary metal in the filtrate is measured in 0.45 micron filtration, thereby obtains the validity that the heavy metal per os in the contaminated soil is taken in.
Embodiment
Gather near the top layer 0～5cm lead-contaminated soil in residential block, Xiao Kou town, Fuyang city, it is air-dry, cross the 0.25mm sieve, its basic physical and chemical: the pH value is 8.0, and the content of organic matter is 1.7%, and content of tatal phosphorus is (with P
2O
5) be 0.13%, available phosphorus content is 26.7mg kg
-1, total lead content is 10000mg kg
-1In soil, add 10000mg kg
-1Hydroxyapatite reduces the validity that per os plumbous in the soil is taken in the hope of reaching, and soil continues stablely to reach 5 months (jede Woche adds deionized water, to keep 20% water cut).
Respectively take by weighing 6 grams with not adding hydroxyapatite with the lead-contaminated soil that adds hydroxyapatite, add 600 milliliters of manual simulation's digestive juices proposed by the invention (by the mean value preparation of aforementioned each concentration range that provides), adopt the designed multi path artificial stomach and intestine simulation system of the present invention, small intestine elementary reaction (pH 7) through 1 hour stomach elementary reaction (pH 1.5) and 4 hours, draw 20 milliliters stomach and small intestine simulation reaction liquid respectively, analyze the content of soluble state lead.
The result shows that for the lead-contaminated soil that does not add hydroxyapatite, the validity of stomach and small intestine stage soil lead is respectively 90% and 21%; For the lead-contaminated soil that adds hydroxyapatite, the validity of stomach and small intestine stage soil lead is respectively 88% and 1.1%, the adding of hydroxyapatite is described, obviously do not reduce the validity of soil lead in human stomach, but the good news is, can reduce soil lead very effectively, reach 20 times approximately in enteral validity, and the main absorption organ of human body is a small intestine, proves that its repairing effect is goodish.The good reproducibility of this test findings, relative error are all in 5%.Therefore, multi path artificial analogue stomach and intestine experimental system proposed by the invention and experiment in vitro method can be measured the validity that the heavy metal-polluted soil per os is taken in effectively, the rapid screening that is used for the plurality of heavy metal contaminated soil is to estimate the human health risk and the soil remediation measure validity of heavy-metal contaminated soil.
A kind of efficient and more reliable artificial stomach and intestine simulation system, the method for the validity that mensuration heavy metal-polluted soil per os is taken in, it comprises the following steps:
The foundation of a, multi path artificial analogue gastronintestinal system;
The collection of b, pedotheque and processing;
C, preparation artificial digestion liquid;
D, soil sample and artificial digestion liquid are added the multi path artificial analogue gastronintestinal system;
E, in control certain reaction condition, through behind the certain hour, in the reaction vessel of the artificial gastrointestinal reaction device of system, gather a certain amount of water sample;
After f, the filtration, measure the soluble state pollutant load in the water sample.
Described method, its described multi path artificial analogue gastronintestinal system comprises argon gas supply subsystem (argon gas steel cylinder, separation panel and gas meter), multi path artificial stomach and intestine analogue reactor (8 reactors) and automatic acid alkalimetric titration instrument.
Described method, top layer 0-5cm soil require is gathered in its described soil collection to be measured and processing, and is air-dry, crosses the 0.25mm sieve, get＜the 0.25mm soil sample carries out next step analysis.
Described method, its described c step is prepared manual simulation's digestive juice, method is: 5 liters of every batch sample preparation simulated gastric fluid, include 0.13～0.17M NaCl, add citric acid 2.0～3.0 grams, malic acid 2.0～3.0 grams, 2.0～2.2 milliliters of lactic acid, 2.0～3.0 milliliters of glacial acetic acids and pepsin 6.0～6.5 grams, the pH value is transferred to 1.3～1.7 with 12M HCl; Standby.
Described method, the method described in its d step is: add 550～650 milliliters artificial digestion liquid and soil to be measured 6 grams of crossing 0.25 mm sieve in each reaction vessel.
Described method, method described in its e step is: reaction vessel is placed 37 ℃ of water-baths, and logical 0.8～1.2 liter of/minute argon gas in artificial digestion liquid is to create anaerobic environment, 80～120 rev/mins of stirrings adopt automatic acid alkalimetric titration instrument to adopt automatic acid alkalimetric titration instrument with HCl or NaHCO
3Control pH value is reacted after 1 hour 1.3～1.7, draws 20 milliliters of reactant liquors with syringe; Then, in each reaction vessel, add NaHCO
3Powder is regulated the pH value 6.8～7.2, adds 0.34～0.36 gram trypsase (pancreatin again, can be available from Sigma company) and 1.1～1.2 gram cholate (bile extract, can be available from Sigma company), in the beginning small intestine stage, adopt automatic acid alkalimetric titration instrument with HCl or NaHCO
3Control pH value after 4 hours, is drawn 20 milliliter reactant liquors with syringe 6.8～7.2.
Described method, the method described in its f step is: simulation stomach or the small intestine reactant liquor gathered are placed centrifuge tube, 10000 rev/mins centrifugal 10 minutes, 0.45 micron filtration adopts atomic absorption method to analyze pollutant load in the filtrate.
Claims (11)
1. a multi path artificial analogue gastronintestinal system assessment soil pollutant to human body validity method is characterized in that comprising the following steps:
A, set up the multi path artificial analogue gastronintestinal system;
The collection of b, pedotheque and processing;
C, preparation artificial digestion liquid;
D, soil sample and artificial digestion liquid are added the multi path artificial analogue gastronintestinal system;
E, in control certain reaction condition, through behind the certain hour, in the reaction vessel of the artificial gastrointestinal reaction device of system, gather a certain amount of water sample;
After f, the filtration, measure the pollutant load in the water sample.
2. the method for claim 1 is characterized in that: set up the multi path artificial analogue gastronintestinal system described in a goes on foot, comprise argon gas supply subsystem, multi path artificial stomach and intestine analogue reactor and automatic acid alkalimetric titration instrument; Wherein, argon gas supply subsystem links to each other with multi path artificial stomach and intestine analogue reactor, 4/5 of the reaction vessel of artificial stomach and intestine analogue reactor places water bath, and the probe of automatic acid alkalimetric titration instrument places the interior reactant liquor of reaction vessel of artificial stomach and intestine analogue reactor.
3. method as claimed in claim 2 is characterized in that: described argon gas supply subsystem, comprise the argon gas steel cylinder, and separation panel and gas meter, the argon gas steel cylinder is communicated with separation panel by reduction valve, is provided with gas meter in a plurality of passages of separation panel.
4. method as claimed in claim 2, it is characterized in that: described artificial stomach and intestine analogue reactor, comprise control panel, glass reaction container and by the stirrer of poly-tetrafluoro bag quilt, a plurality of channel connections of control panel and separation panel, reaction vessel and stirrer and control panel are connected, and the lower end of passage and stirrer place the bottom in the reaction vessel.
5. method as claimed in claim 2 is characterized in that: the probe of described automatic acid alkalimetric titration instrument places the interior reactant liquor of reaction vessel of artificial stomach and intestine analogue reactor, and its liquid feeding head places reactant liquor top 2～3cm.
6. as claim 4 or 5 described methods, it is characterized in that: described reaction vessel is 6～8.
7. the method for claim 1 is characterized in that: the collection and the processing of pedotheque described in the b step are to gather top layer 0～5cm soil, and be air-dry, crosses the 0.25mm sieve, get＜the 0.25mm soil sample carries out next step analysis.
8. the method for claim 1, it is characterized in that: the liquid of preparation artificial digestion described in the c step, its method is: 5 liters of every batch sample preparation simulated gastric fluid, include 0.13～0.17MNaCl, add citric acid 2.0～3.0 grams, malic acid 2.0～3.0 grams, 2.0～2.2 milliliters of lactic acid, 2.0～3.0 milliliters of glacial acetic acids and pepsin 6.0～6.5 grams, the pH value is transferred to 1.3～1.7 with 12M HCl; Standby.
9. the method for claim 1 is characterized in that: in d goes on foot, is soil to be measured 6 grams that add 550～650 milliliters artificial digestion liquid respectively and cross 0.25 mm sieve in each reaction vessel.
10. the method for claim 1, it is characterized in that: in e goes on foot, reaction vessel is placed 37 ℃ of water-baths, speed with 0.8～1.2 liter/minute in artificial digestion liquid feeds argon gas, to create anaerobic environment, with 80～120 rev/mins of stirrings, the beginning stomach stage, adopt automatic acid alkalimetric titration instrument control pH value 1.3～1.7, react after 1 hour, draw 20 milliliters of simulation stomach reactant liquors with syringe; Then, in each reaction vessel, add NaHCO respectively
3Powder is regulated the pH value 6.8～7.2, adds 0.34～0.38 gram trypsase and 1.1～1.3 gram cholate again, in the beginning small intestine stage, adopts automatic acid alkalimetric titration instrument with HCl or NaHCO
3Control pH value after 4 hours, is drawn 20 milliliter reactant liquors with syringe 6.8～7.2.
11. the method for claim 1, it is characterized in that: in the step, simulation stomach or the small intestine reactant liquor of being gathered placed centrifuge tube at f, centrifugal 10 minutes with 10000 rev/mins, 0.45 micron filters, and adopts the pollutant load in the atomic absorption method analysis filtrate.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101968480A (en) * | 2009-10-27 | 2011-02-09 | 漳州师范学院 | Simulation and detection method for content of microelements in medicine capable of being absorbed by human body |
| CN103439476A (en) * | 2013-08-30 | 2013-12-11 | 北京市环境保护科学研究院 | Turnover testing device and method for heavy metal availability by stimulating human digestion characteristics |
| CN103926375A (en) * | 2014-03-12 | 2014-07-16 | 南昌大学 | Device for simulating human digestive and glycolytic system and application method thereof |
| CN107814436A (en) * | 2017-09-12 | 2018-03-20 | 天津大学 | A kind of multichannel anaerobic-aerobic alternation response experimental provision and experimental method |
| CN107978368A (en) * | 2017-12-01 | 2018-05-01 | 南京大学 | A kind of heavy metal health risk assessment method in depositing dust based on metal SBET |
| CN108364553A (en) * | 2018-02-08 | 2018-08-03 | 江南大学 | A kind of small enteron aisle digestion analogy method of human stomach-and device |
| CN108535046A (en) * | 2018-05-10 | 2018-09-14 | 宁波市鄞州区达之丰工业产品设计有限公司 | A kind of organic pollution sampling analysis equipment |
| CN109975524A (en) * | 2019-05-08 | 2019-07-05 | 江苏省环境科学研究院 | It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method |
| CN113009008A (en) * | 2021-01-26 | 2021-06-22 | 南京大学 | In-vitro device and method for measuring bioavailability of hydrophobic organic pollutants in soil |
| CN113406298A (en) * | 2020-03-16 | 2021-09-17 | 中国科学院、水利部成都山地灾害与环境研究所 | Method for detecting fertility of soil |
| EP4184165A4 (en) * | 2020-12-30 | 2024-04-17 | Universidad Tecnológica Metropolitana | Portable modular gastrointestinal simulator system that quantifies bioaccessibility of metals in a sample remotely and in real time, and method |
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| CN101968480B (en) * | 2009-10-27 | 2013-09-11 | 闽南师范大学 | Simulation and detection method for content of microelements in medicine capable of being absorbed by human body |
| CN101968480A (en) * | 2009-10-27 | 2011-02-09 | 漳州师范学院 | Simulation and detection method for content of microelements in medicine capable of being absorbed by human body |
| CN103439476A (en) * | 2013-08-30 | 2013-12-11 | 北京市环境保护科学研究院 | Turnover testing device and method for heavy metal availability by stimulating human digestion characteristics |
| CN103439476B (en) * | 2013-08-30 | 2015-07-15 | 北京市环境保护科学研究院 | Turnover testing device and method for heavy metal availability by stimulating human digestion characteristics |
| CN103926375A (en) * | 2014-03-12 | 2014-07-16 | 南昌大学 | Device for simulating human digestive and glycolytic system and application method thereof |
| CN107814436A (en) * | 2017-09-12 | 2018-03-20 | 天津大学 | A kind of multichannel anaerobic-aerobic alternation response experimental provision and experimental method |
| CN107814436B (en) * | 2017-09-12 | 2021-07-27 | 天津大学 | Multichannel anaerobic-aerobic alternative reaction experimental device and experimental method |
| CN107978368A (en) * | 2017-12-01 | 2018-05-01 | 南京大学 | A kind of heavy metal health risk assessment method in depositing dust based on metal SBET |
| CN107978368B (en) * | 2017-12-01 | 2022-06-21 | 南京大学 | Metal SBET-based health risk assessment method for heavy metals in dust fall |
| CN108364553B (en) * | 2018-02-08 | 2020-04-14 | 江南大学 | Human body stomach-small intestinal tract digestion simulation method and device |
| CN108364553A (en) * | 2018-02-08 | 2018-08-03 | 江南大学 | A kind of small enteron aisle digestion analogy method of human stomach-and device |
| CN108535046A (en) * | 2018-05-10 | 2018-09-14 | 宁波市鄞州区达之丰工业产品设计有限公司 | A kind of organic pollution sampling analysis equipment |
| CN108535046B (en) * | 2018-05-10 | 2019-08-20 | 东阳市诺达电子科技有限公司 | A kind of organic pollutant sampling analysis equipment |
| CN109975524A (en) * | 2019-05-08 | 2019-07-05 | 江苏省环境科学研究院 | It is a kind of simulation human stomach digest feature heavy metal biological can give system safety testing device and method |
| CN113406298A (en) * | 2020-03-16 | 2021-09-17 | 中国科学院、水利部成都山地灾害与环境研究所 | Method for detecting fertility of soil |
| EP4184165A4 (en) * | 2020-12-30 | 2024-04-17 | Universidad Tecnológica Metropolitana | Portable modular gastrointestinal simulator system that quantifies bioaccessibility of metals in a sample remotely and in real time, and method |
| CN113009008A (en) * | 2021-01-26 | 2021-06-22 | 南京大学 | In-vitro device and method for measuring bioavailability of hydrophobic organic pollutants in soil |
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