CN209669226U - A kind of cell culture bags convenient for sampling and fluid infusion - Google Patents
A kind of cell culture bags convenient for sampling and fluid infusion Download PDFInfo
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- CN209669226U CN209669226U CN201920372079.9U CN201920372079U CN209669226U CN 209669226 U CN209669226 U CN 209669226U CN 201920372079 U CN201920372079 U CN 201920372079U CN 209669226 U CN209669226 U CN 209669226U
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- fluid infusion
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Abstract
The utility model discloses a kind of cell culture bags convenient for sampling and fluid infusion, including culture bag ontology, liquid supplementation pipe and probe tube are respectively equipped in the opposite both ends of culture bag ontology, liquid supplementation pipe is equipped with first and stops liquid valve, fluid infusion cylinder is connected in the free end of liquid supplementation pipe, fluid infusion cylinder has dismountable cover far from one end of liquid supplementation pipe, be fixed on the outer wall of fluid infusion cylinder cover upward when fluid infusion cylinder can be supported in vertically to support construction in a plane；Probe tube be equipped with second only liquid valve in being parallel with multiple branch pipes on the second only probe tube below liquid valve a sampling bag is fixed on the free end of each branch pipe, and each branch pipe is equipped with third and stops liquid valve.Cell culture bags can complete sampling without asepsis injector and fluid infusion operates, and can reduce cell contamination probability.
Description
Technical field
The utility model relates to Bioexperiment clothing arts more particularly to it is a kind of convenient for sampling and fluid infusion cell culture
Bag.
Background technique
Cellular immunotherapy is the technology that medical oncology field is quickly grown, and is passed greatly after operation, radiotherapy, chemotherapy three
Rule the 4th kind for the treatment of technology after treating.Cellular immunotherapy isolates immunocyte by acquisition autologous patient peripheral blood, leads to
External evoked culture is crossed, its massive amplification is made, final feed back kills tumour cell in patient's body.
High density amplification generally is carried out to immunocyte using cell culture bags at present, cell culture bags are by special substance system
At permeable watertight, compared to traditional Tissue Culture Flask, gas exchange area is big, sertoli cell high-density growth.But
In cell cultivation process, sample detection cell phenotype and supplement fresh culture belong to essential operation.
Existing cell culture bags use channel of the sterile PVC pipeline as supplementing culture medium, need opening conduits end lid,
The disposable sterilized injector of connection removal piston, pulls up syringe empty barrel after injecting culture medium again, lid is screwed back, to complete
Fluid infusion.Fluid infusion is complicated for operation cumbersome, increases cell contamination probability, the fluid infusion more than once in culture, it is necessary to repeatedly uncap
New syringe is accessed, is resulted in waste of resources.
It also needs to be inserted into sample tap by band needle sterile syringe if sampling, extracts cell suspension out, or directly
It flows out part cell suspension in inlet pipe, completes sampling.Sampling operation is complicated as a result, need to increase by band needle applicator
Add cell contamination probability, while being a kind of security risk to operator.
Utility model content
The technical problem to be solved by the utility model is to provide one kind can complete to sample without asepsis injector
It is operated with fluid infusion, the cell culture bags convenient for sampling and fluid infusion of cell contamination probability can be reduced.
In order to solve the above technical problems, technical solution adopted in the utility model is:
A kind of cell culture bags convenient for sampling and fluid infusion, including culture bag ontology, in the opposite both ends of culture bag ontology
It is respectively equipped with liquid supplementation pipe and probe tube, it is characterised in that: the liquid supplementation pipe is equipped with first and stops liquid valve, in the free end of liquid supplementation pipe
It is connected with fluid infusion cylinder, the fluid infusion cylinder has dismountable cover far from one end of liquid supplementation pipe, consolidates on the outer wall of the fluid infusion cylinder
Surely have cover upward when fluid infusion cylinder can be supported in vertically to support construction in a plane；The probe tube is equipped with second
Only liquid valve is fixed with one on the free end of each branch pipe and takes in being parallel with multiple branch pipes on the second only probe tube below liquid valve
Sample bag, and each branch pipe is equipped with third and stops liquid valve.
A further technical solution lies in: the support construction includes uniformly being fixed on fluid infusion drum outer wall extremely in circumferential direction
Few three support rods, the support rod stretch out outside fluid infusion cylinder, and multiple support rods, which expand outwardly, encloses the tapered structure of structure.
A further technical solution lies in: the support rod includes the casing seat being fixed on fluid infusion drum outer wall and grafting
In the inserted link in casing seat.
A further technical solution lies in: the cover is connect with fluid infusion cylinder using 1/3 corner-type structure.
A further technical solution lies in: the fluid infusion cylinder is connect with liquid supplementation pipe using dismountable mode.
A further technical solution lies in: the tapered structure in one end that the fluid infusion cylinder is connect with liquid supplementation pipe.
A further technical solution lies in: the third stops liquid valve using dismountable liquid stopping clamp.
A further technical solution lies in: the end that the fluid infusion cylinder is connect with liquid supplementation pipe be equipped with can adjust fluid infusion cylinder with
The flow regulator of liquid supplementation pipe connection openings of sizes.
A further technical solution lies in: the flow regulator is flow control valve.
A further technical solution lies in: the fluid infusion cylinder is equipped with the scale strip being axially arranged along it.
The beneficial effects of adopting the technical scheme are that
Using the culture bag when carrying out fluid infusion operation, connection asepsis injector outer cylinder is not needed, is not needed by bracket
Or hand-held fixed syringe cylinder, only the fluid infusion cylinder for being used for fluid infusion need to be stood on operating table surface, cell can be trained by fluid infusion cylinder
It supports bag and carries out fluid infusion operation, it is simple to operate, reduce pollution probability.After the completion of fluid infusion, fluid infusion cylinder is just being put using cover
In in operating table surface, fluid infusion liquid within the cartridge is thus blocked to flow in culture bag.
When fluid infusion cylinder carries out fluid infusion operation, it is supported on station using support construction, avoids liquid supplementation pipe and exist
It is bent with fluid infusion cylinder junction, so that fluid infusion is more smooth, while also avoiding the phenomenon that junction cracking causes leakage,
And fluid infusion cylinder is placed steadily, and it is hand steered without operator.
In addition, being equipped with first in liquid supplementation pipe, only liquid valve, the setting that should stop liquid valve can prevent the culture medium in culture bag
Matter is back in fluid infusion cylinder, plays protective action, avoids the pollution to fluid infusion liquid within the cartridge.
Using the culture bag when being sampled operation, bag body inner cell suspension is first mixed, second is then opened and stops liquid valve,
And open the third on a certain branch pipe and stop liquid valve, flow into the cell suspension in it in sampling bag by squeezing bag body, then by the
Two, three only liquid valve closing, is finally fallen branch pipe heat seal with hand-held heat-sealing machine, that is, completes sampling.Entire sampling process is being sealed completely
It is completed under closed state, without using band needle applicator, avoids pollution caused by external devices and opening manipulation, also avoid
Syringe needle accidentally injures the risk of operator.
Detailed description of the invention
Utility model will be further described in detail below with reference to the attached drawings and specific embodiments.
Fig. 1 is the structural schematic diagram of the utility model.
Specific embodiment
Below with reference to the attached drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out clear
Chu is fully described by, it is clear that described embodiment is a part of the embodiment of the utility model, rather than whole implementation
Example.Based on the embodiments of the present invention, those skilled in the art are obtained without making creative work
Every other embodiment, fall within the protection scope of the utility model.
Many details are explained in the following description in order to fully understand the utility model, but this is practical new
Type can also be implemented using other than the one described here other way, and those skilled in the art can be without prejudice to this reality
With doing similar popularization in the case where novel intension, therefore the utility model is not limited by the specific embodiments disclosed below.
As shown in Figure 1, a kind of cell culture bags convenient for sampling and fluid infusion, including culture bag ontology 1, in culture bag ontology
1 opposite both ends are respectively equipped with liquid supplementation pipe 2 and probe tube 3.The liquid supplementation pipe 2 is equipped with first and stops liquid valve 21, in liquid supplementation pipe 2
Free end is connected with fluid infusion cylinder 4, and the fluid infusion cylinder 4 has dismountable cover 41, the fluid infusion cylinder far from one end of liquid supplementation pipe 2
Be fixed on 4 outer wall cover 41 upward when fluid infusion cylinder 4 can be supported in vertically to support construction in a plane；It is described to take
Sample pipe 3 is equipped with the second only liquid valve 31, in being parallel with multiple branch pipes 32, Mei Yizhi on the second only probe tube 3 of the lower section of liquid valve 31
A sampling bag 33 is fixed on the free end of pipe 32, and each branch pipe 32 is equipped with third and stops liquid valve 34.
Using the culture bag when carrying out fluid infusion operation, the fluid infusion cylinder 4 for being used for fluid infusion is first found into (cover 41 is upward) in behaviour
Make table top, open cover 41 to fluid infusion cylinder 4 and inject replenisher, then cover 41 is installed on fluid infusion cylinder 4 again, avoids extraneous miscellaneous
Matter enters fluid infusion cylinder 4 and pollutes, and opens first and stops liquid valve 21, can pass through the 4 pairs of cell culture of fluid infusion cylinder using liquid level difference
Bag carries out fluid infusion operation.Fluid infusion operation does not need connection asepsis injector outer cylinder, does not need by bracket or hand-held fixed note
Emitter cylinder, it is simple to operate, reduce pollution probability.After the completion of fluid infusion, closes first and stop liquid valve 21, tighten cover 41, by
It in there is cover 41 that can be covered on 4 opening of fluid infusion cylinder, therefore has mended the fluid infusion cylinder 4 after liquid and can arbitrarily place, while the first only liquid
After the closing of valve 21 and cover 41 are covered on 4 opening of fluid infusion cylinder, extraneous pressure can also be blocked, therefore culture bag can be blocked
Liquid in ontology 1 flows back in fluid infusion cylinder.
Using the culture bag when being sampled operation, bag body inner cell suspension is first mixed, second is then opened and stops liquid valve
31, and the third opened on a certain branch pipe 32 stops liquid valve 34, so that the gas in it is discharged into culture bag sheet by squeezing sampling bag 33
In body 1, sampling bag 33 is then unclamped, while gently squeezing culture bag ontology 1 flows into the cell suspension in it in sampling bag 33,
Second and third is stopped liquid valve again to close, is finally fallen 32 heat seal of branch pipe with hand-held heat-sealing machine, is i.e. completion sampling operation.It has sampled
Cheng Hou, opening second, only liquid valve 31, the third opened simultaneously on another branch pipe 32 are stopped liquid valve 34, are connected on the branch pipe 32 by squeezing
The cell suspension remained in probe tube 3 can be squeezed and is back in culture bag ontology 1 by the sampling bag 33 connect, so can be to avoid
Cell suspension is remained in probe tube 3 and is impacted to subsequent samples.Entire sampling process is completed under completely enclosed state,
Without using band needle applicator, pollution caused by external devices and opening manipulation is avoided, syringe needle is also avoided and accidentally injures behaviour
Make the risk of personnel.Since quantity of sampling quantity is uncertain in incubation, sampling bag 33 can also be connected to certain by sterile bulb-tubulating machine
On one branch pipe 32.
When fluid infusion cylinder 4 carries out fluid infusion operation, it is supported on station using support construction, avoids liquid supplementation pipe 2
It is bent with 4 junction of fluid infusion cylinder, so that fluid infusion is more smooth, while also avoiding junction cracking and causing showing for leakage
As, and fluid infusion cylinder 4 is placed steadily, and it is hand steered without operator.
In addition, being equipped with first in liquid supplementation pipe 2, only liquid valve 21, the setting that should stop liquid valve can prevent the culture in culture bag
Matrix is back in fluid infusion cylinder 4, plays protective action, avoids the pollution to liquid in fluid infusion cylinder 4.
Specifically, support construction includes at least three support rods 5 being uniformly fixed on 4 outer wall of fluid infusion cylinder in circumferential direction, institute
It states support rod 5 to stretch out outside fluid infusion cylinder 4, when so that support rod 5 and operating table surface contacting, makes fluid infusion cylinder 4 that must locate liquid mouth hanging, and
Multiple support rods 5, which expand outwardly, encloses the tapered structure of structure, so that it is more stable to the support of fluid infusion cylinder 4.Wherein, it supports
Bar 5 is at least three, constitutes gusseted, to guarantee the stability of its support.
In addition, support rod 5 is made into dismountable structure, support rod 5 includes the casing seat being fixed on 4 outer wall of fluid infusion cylinder
51 and the inserted link 52 that is plugged in casing seat 51.Inserted link 52 can be extracted after completing fluid infusion from casing seat 51, be dismantled
Fluid infusion cylinder 4 after support rod 5 can be directly placed in incubator together with culture bag ontology 1, can be effectively reduced to empty in incubator
Between use, avoid occupy incubator in excessive space and cause that more cells can not be cultivated, and also facilitate to culture bag
Put.
Cover 41 is connect with fluid infusion cylinder 4 using 1/3 corner-type structure, and 4 liquid feeding opening of fluid infusion cylinder, which uses, meets man-machine work
1/3 corner that effect learns design opens and closes formula cover 41, specifically circumferential on the outer wall of fluid infusion cylinder 4 and uniformly distributed there are three be in
The protrusion of triangle, the slot that motion profile matches when being equipped on the inner wall of cover 41 with the protrusion relative to the rotation of cover 41
Body realizes the fixation that cover 41 is connect with fluid infusion cylinder 4 when the protrusion of triangle is stuck in groove body.Pass through 1/3 corner-type structure
Connection can be achieved one hand and remove cover 41 from fluid infusion cylinder 4, and cover 41 need to only be rotated to 1/3 circle, culture medium is poured into
In fluid infusion cylinder 4, culture medium can flow into bag body through inlet tube, and easy to operate, operation can be completed in one.And in order to guarantee cover
41 airtightness connecting with fluid infusion cylinder 4 are equipped with circumferential sealing ring in 41 inner wall of cover.
Fluid infusion cylinder 4 is connect with liquid supplementation pipe 2 using dismountable mode, specifically, can be used in liquid supplementation pipe 2 and fluid infusion cylinder 4
Internal screw thread is set on the inner sidewall of connecting pin, fluid infusion cylinder 4 be connected with liquid supplementation pipe 2 hold be equipped with match with internal screw thread it is outer
Screw thread, so that the two be made to be threadedly coupled.It can be achieved to replace fluid infusion cylinder 4.
The tapered structure in one end that fluid infusion cylinder 4 is connect with liquid supplementation pipe 2 can avoid the residual of culture medium in fluid infusion cylinder 4.
After sampling, sampling bag 33 is separated with 32 heat seal of branch pipe, and third thereon, which stops liquid valve 34 no longer, to be had using work
With so third stops liquid valve 34, and it is sharp can to remove liquid stopping clamp repetition after the separation of sampling bag 33 using dismountable liquid stopping clamp
With avoiding the waste of consumptive material.
Fluid infusion cylinder 4 can be adjusted and be connected to openings of sizes with liquid supplementation pipe 2 by being equipped in the end that fluid infusion cylinder 4 is connect with liquid supplementation pipe 2
Flow regulator, the flow of culture medium flowed into culture bag can be adjusted.Specifically, flow regulator is stream
Adjustable valve.
It is equipped with the scale strip being axially arranged along it, on fluid infusion cylinder 4 convenient for observation fluid infusion situation.
The above is only the preferred embodiments of the utility model, anyone content according to the present utility model is to the utility model
A little simple modification, deformation and the equivalent replacement made each falls within the protection scope of the utility model.
Claims (10)
1. a kind of cell culture bags convenient for sampling and fluid infusion, including culture bag ontology (1), opposite in culture bag ontology (1)
Both ends are respectively equipped with liquid supplementation pipe (2) and probe tube (3), it is characterised in that: the liquid supplementation pipe (2) is equipped with first and stops liquid valve
(21), it is connected with fluid infusion cylinder (4) in the free end of liquid supplementation pipe (2), the fluid infusion cylinder (4) has far from the one end of liquid supplementation pipe (2)
Dismountable cover (41), be fixed on the outer wall of the fluid infusion cylinder (4) cover (41) upward when can be perpendicular by fluid infusion cylinder (4)
The support construction being directly supported in a plane；The probe tube (3) is equipped with second only liquid valve (31), only liquid valve (31) in second
It is parallel with multiple branch pipes (32) on the probe tube (3) of lower section, is fixed with a sampling bag (33) on the free end of each branch pipe (32),
And each branch pipe (32) is equipped with third and stops liquid valve (34).
2. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 1, it is characterised in that: the support knot
Structure includes at least three support rods (5) being uniformly fixed on fluid infusion cylinder (4) outer wall in circumferential direction, and the support rod (5), which is stretched out, mends
Outside, multiple support rods (5) expand outwardly encloses the tapered structure of structure to fluid cylinder (4).
3. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 2, it is characterised in that: the support rod
It (5) include the casing seat (51) being fixed on fluid infusion cylinder (4) outer wall and the inserted link (52) being plugged in casing seat (51).
4. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 1, it is characterised in that: the cover
(41) it is connect with fluid infusion cylinder (4) using 1/3 corner-type structure.
5. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 1, it is characterised in that: the fluid infusion cylinder
(4) it is connect with liquid supplementation pipe (2) using dismountable mode.
6. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 1, it is characterised in that: the fluid infusion cylinder
(4) the tapered structure in one end being connect with liquid supplementation pipe (2).
7. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 1, it is characterised in that: the third is stopped
Liquid valve (34) uses dismountable liquid stopping clamp.
8. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 1, it is characterised in that: the fluid infusion cylinder
(4) end connecting with liquid supplementation pipe (2), which is equipped with, can adjust the flow tune that fluid infusion cylinder (4) is connected to openings of sizes with liquid supplementation pipe (2)
Regulating device.
9. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 8, it is characterised in that: the flow tune
Regulating device is flow control valve.
10. a kind of cell culture bags convenient for sampling and fluid infusion according to claim 1, it is characterised in that: the fluid infusion
Cylinder (4) is equipped with the scale strip being axially arranged along it.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920372079.9U CN209669226U (en) | 2019-03-22 | 2019-03-22 | A kind of cell culture bags convenient for sampling and fluid infusion |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920372079.9U CN209669226U (en) | 2019-03-22 | 2019-03-22 | A kind of cell culture bags convenient for sampling and fluid infusion |
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| CN209669226U true CN209669226U (en) | 2019-11-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
-
2019
- 2019-03-22 CN CN201920372079.9U patent/CN209669226U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
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